Method for preparing amino acid-containing water-soluble fertilizer by using livestock and poultry slaughter blood

Abstract

The invention relates to the field of preparation of amino acid water-soluble fertilizers, and discloses a method for preparing an amino acid-containing water-soluble fertilizer by using slaughtering blood of bred livestock and poultry, which comprises the following steps: collecting the blood of the bred livestock and poultry in a slaughter house through a blood collection container, and adding an anticoagulant to obtain an anticoagulant raw material; the preparation method comprises the following steps: heating and disinfecting an anticoagulant raw material, and then adding bacillus subtilis and monopotassium phosphate to carry out enzymolysis treatment, so as to obtain a feed liquid after enzymolysis; then filtering, complexing treatment, ultraviolet sterilization treatment and sterilization are carried out in sequence; carrying out amino acid detection on the sterilized material liquid subjected to enzymolysis, and adding other nutrients to form an organic liquid fertilizer containing amino acid; and finally, carrying out preservative treatment, packaging and warehousing, and storing in a shade place to complete the preparation process of the amino acid water-soluble fertilizer. The invention solves the problems that the existing amino acid water-soluble fertilizer preparation raw materials are less, the amino acid water-soluble fertilizer preparation process is longer, and the environment is polluted due to the discharge of livestock and poultry slaughtering blood waste.

Description

Technical Field

[0001] The invention relates to the field of preparation of amino acid water-soluble fertilizer, and in particular to a method for preparing amino acid-containing water-soluble fertilizer by utilizing slaughtered blood of livestock and poultry. Background Art

[0002] Amino acid water-soluble fertilizer is a new generation of amino acid liquid fertilizer that uses agricultural and sideline products as the main raw materials and is produced by bio-fermentation technology and membrane concentration technology. It integrates multiple functions, high technology and environmental protection. It has the effects of stimulating plant growth, increasing crop yields, improving crop quality, increasing protein and vitamin content, promoting the activation of soil microbial activity, and enhancing crop resistance. The large-scale application of chemical fertilizers has played an important role in the development of my country's agriculture.

[0003] At present, there are few raw materials for the preparation of amino acid water-soluble fertilizers, which makes it difficult to meet market demand. The preparation process is relatively complicated and cannot fully adapt to the huge market. It often takes a long time. Therefore, it is urgent to find a raw material for preparing amino acid water-soluble fertilizers. How to simply and efficiently obtain amino acid-containing water-soluble fertilizers is a technical problem that the existing technology needs to solve. Summary of the invention

[0004] The present invention aims to provide a method for preparing amino acid-containing water-soluble fertilizer using slaughtered blood of livestock and poultry, so as to solve the problems that the existing amino acid water-soluble fertilizer preparation process is relatively long, and the discharge of waste blood from slaughtered livestock and poultry causes environmental pollution.

[0005] In order to achieve the above object, the present invention provides the following method:

[0006] The present invention provides a method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood:

[0007] S1: collecting blood from livestock and poultry in a slaughterhouse through a blood collection container, adding an anticoagulant to the blood from livestock and poultry in the slaughterhouse to obtain an anticoagulant raw material;

[0008] S2: heating and disinfecting the anticoagulant raw material, adding Bacillus subtilis and fermentation additives for enzymolysis to obtain a hydrolyzed feed liquid;

[0009] S3: filtering the enzymatically hydrolyzed liquid, performing a complexation treatment, and then performing an ultraviolet disinfection treatment to sterilize the enzymatically hydrolyzed liquid;

[0010] S4: performing amino acid detection on the sterilized and enzymatically hydrolyzed feed liquid, and then adding other nutrients to form an organic liquid fertilizer containing amino acids;

[0011] S5: performing an antiseptic treatment on the amino acid-containing organic liquid fertilizer, packaging the amino acid-containing organic liquid fertilizer after the antiseptic treatment, storing it in a cool place.

[0012] Preferably, the livestock and poultry blood in step S1 is one or more of pig blood, sheep blood, cow blood and chicken and duck blood.

[0013] Preferably, the ratio of the livestock and poultry blood to the anticoagulant in step S1 is 9:1.

[0014] Preferably, in step S1, an anticoagulant is added to the blood of livestock and poultry in the slaughterhouse to obtain the mixing conditions of the anticoagulant raw material as follows: an anticoagulant is added to the blood of livestock and poultry in the slaughterhouse, and the blood is fully stirred and polished to perform anti-coagulation treatment.

[0015] Preferably, the step of heating and disinfecting the anticoagulant raw material, adding Bacillus subtilis and a fermentation additive for enzymatic hydrolysis to obtain an enzymatically hydrolyzed liquid comprises: heating the anticoagulant raw material to 53°C±1°C, disinfecting it, then adding Bacillus subtilis and potassium dihydrogen phosphate, fermenting it to 59°C±1°C, then adding potassium dihydrogen phosphate, and fermenting it for 72 hours to obtain an enzymatically hydrolyzed liquid.

[0016] Preferably, in step S3, the complexing treatment process is: fermenting and filtering the enzymatically hydrolyzed liquid, adding the complexing composition therein, and performing complexing for 40 minutes.

[0017] Preferably, the complex composition is 15% imported composite amino acid powder, 2% zinc sulfate, and 2% borax.

[0018] Preferably, the complex composition is 30% of composite amino acid powder, 34-35% of zinc sulfate, 10-12% of borax and the balance of deionized water.

[0019] Preferably, in step S4, the sterilized and enzymatically hydrolyzed liquid is subjected to amino acid detection, and then other nutrients are added to form an organic liquid fertilizer containing amino acids, until the concentration of the amino acid detected is 10% higher than the national standard, and then the addition of other nutrients to form an organic liquid fertilizer containing amino acids is stopped.

[0020] Preferably, the step of subjecting the amino acid-containing organic liquid fertilizer to an antiseptic treatment, packaging and warehousing the amino acid-containing organic liquid fertilizer after the antiseptic treatment, and storing it in a cool place comprises: adding a plant-based preservative to the amino acid-containing organic liquid fertilizer, wherein the added amount is about 0.1%; packaging and warehousing the amino acid-containing organic liquid fertilizer that has been tested to meet national standards after the antiseptic treatment, and storing it in a cool place.

[0021] The beneficial effects of the present invention are as follows: the present invention collects the blood of livestock and poultry in slaughterhouses in a centralized manner, then adds fermentation materials and ferments with Bacillus microorganisms, fully hydrolyzes the protein in the blood into amino acid form under specified conditions, and finally forms water-soluble fertilizer. The entire treatment process does not produce secondary treatment tailings, which is beneficial to solving the environmental pollution problem caused by the current discharge of waste blood. At the same time, the fertilizer produced can effectively increase crop yields. The present invention takes into account that the fishy smell of blood is heavy, which may easily lead to excessive odor in the finished fertilizer produced later, thereby causing discomfort to the user. The deodorization and deodorization operation is beneficial to removing the fishy smell in the liquid feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0023] Figure 1 The present invention provides a flow chart of a method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood.

[0024] Figure 2 The present invention provides a production process flow chart of a method for preparing amino acid-containing water-soluble fertilizer by using slaughtered livestock and poultry blood. DETAILED DESCRIPTION

[0025] In order to make the technical personnel in the technical field better understand the scheme of the present invention, the technical scheme in the embodiment of the present invention will be clearly and completely described below in combination with the drawings in the embodiment of the present invention. Obviously, the described embodiment is only a part of the embodiment of the present invention, not all the embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The terms "first", "second", etc. in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish different objects rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, device, product or end including a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units that are not listed, or may optionally include other steps or units inherent to these processes, methods, products or ends.

[0027] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present invention. The appearance of the phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0028] At present, there are few raw materials for the preparation of amino acid water-soluble fertilizers, which makes it difficult to meet market demand. The preparation process is relatively complicated and cannot fully adapt to the huge market. It often takes a long time. Therefore, it is urgent to find a raw material for preparing amino acid water-soluble fertilizers. How to simply and efficiently obtain amino acid-containing water-soluble fertilizers is a technical problem that the existing technology needs to solve.

[0029] The present invention aims to provide a method for preparing amino acid-containing water-soluble fertilizer using slaughtered blood of livestock and poultry, so as to solve the problems that the existing amino acid water-soluble fertilizer preparation process is relatively long, and the discharge of waste blood from slaughtered livestock and poultry causes environmental pollution.

[0030] The specific embodiment of the present invention provides a method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood. Figure 1 and Figure 2 As shown, the following steps are included:

[0031] S1: Collecting the blood of livestock and poultry in a slaughterhouse through a blood collection container, adding an anticoagulant to the blood of livestock and poultry in the slaughterhouse to obtain an anticoagulant raw material.

[0032] In an embodiment of the present invention, the blood of farmed livestock and poultry is one or more of pig blood, sheep blood, cow blood and chicken and duck blood; the usage ratio of farmed livestock and poultry blood to anticoagulant is 9:1; anticoagulant is added to the blood of farmed livestock and poultry in a slaughterhouse, and the mixing conditions for obtaining the anticoagulant raw material are: anticoagulant is added to the blood of farmed livestock and poultry in a slaughterhouse, and the blood is fully stirred and polished to perform anti-coagulation treatment.

[0033] S2: After heating and disinfecting the anticoagulant raw material, Bacillus subtilis and fermentation additives are added to perform enzymatic hydrolysis to obtain a hydrolyzed feed liquid.

[0034] In the embodiment of the present invention, the anticoagulant raw material is heated to 53°C±1°C, disinfected, and then Bacillus subtilis and potassium dihydrogen phosphate are added. When the temperature of the fermentation temperature reaches 59°C±1°C, potassium dihydrogen phosphate is added and fermented for 72 hours to obtain a liquid after enzymolysis. The advantage of biological enzymolysis technology is that the substance is self-decomposed through the catalytic action of the enzyme. The ability of the enzyme to catalyze chemical reactions is called enzyme activity (or enzyme activity). Enzyme activity can be regulated and controlled by a variety of factors, so that the organism can adapt to changes in external conditions and maintain life activities. By utilizing the different catalytic actions of different enzymes, the characteristics of different active components of the substance can be mastered, and the active substances of the substance can be extracted. Traditional material extraction adopts high-temperature continuous lysis or low-temperature quick freezing, so Most of the active substances in the substance will be destroyed, causing the extract to lose its activity and affecting the efficacy; the organism is composed of cells, and each cell can only show various life activities and metabolism in the body due to the presence of enzymes; enzymes are catalysts for metabolism in the human body, and only with the presence of enzymes can various biochemical reactions be carried out in the human body; the more enzymes in the human body, the more complete, the healthier its life; when there are no active enzymes in the human body, life will end; most human diseases are related to enzyme deficiency or synthesis disorders; biological enzymatic hydrolysis technology can make material extracts rich in active enzymes, promote human metabolism, and its application in human health engineering is very effective; enzymatic hydrolysis is based on the reaction principle of enzyme-catalyzed substrate conversion into product. Enzymes are a class of proteins with high specificity and high catalytic activity, which can accelerate the rate of substrate conversion into product. In the enzymatic hydrolysis method, the substrate is converted into product by controlling the reaction conditions (such as temperature, pH value, etc.) and substrate concentration, and the reaction rate is determined by measuring the change in product concentration; before conducting an enzymatic hydrolysis experiment, a substrate solution of appropriate concentration needs to be prepared. The substrate can be an organic molecule naturally present in organisms or artificially synthesized, or it can be other biological macromolecules (such as proteins, nucleic acids, etc.); the nature and concentration of the substrate should be determined according to the specific experimental purpose and the characteristics of the enzyme being studied. The enzyme solution prepared in advance is added to the substrate solution to react with the substrate. The amount of enzyme added should be determined according to the characteristics and reaction rate of the enzyme being studied. Generally, experiments with different enzyme concentrations are required to determine the most suitable conditions;

[0035] When conducting enzymatic hydrolysis experiments, it is necessary to control some important reaction conditions, such as temperature, pH value, etc. These conditions are crucial to maintaining enzyme activity and stability. Generally, the best reaction conditions can be determined by conducting experiments at different temperatures and pH values.

[0036] In enzymatic hydrolysis experiments, it is necessary to determine the appropriate reaction time. According to the enzyme catalytic rate and substrate conversion rate under study, samples can be collected at different time points, and the optimal reaction time can be determined by measuring the product concentration.

[0037] S3: Filter the enzymatically hydrolyzed liquid, perform complexation treatment, and then perform ultraviolet disinfection treatment to sterilize the enzymatically hydrolyzed liquid.

[0038] In the embodiment of the present invention, the complexing process is: ferment and filter the enzymatically hydrolyzed liquid, add a complexing composition therein, and carry out complexing for 40 minutes; the complexing composition is 15% imported composite amino acid powder, 2% zinc sulfate, 2% borax or the complexing composition is 30% composite amino acid powder, 34-35% zinc sulfate, 10-12% borax and the balance deionized water; the complexing reaction refers to the process in which two or more substances in the solution form a complex through chemical reactions. The complex is a stable structure formed by the central metal ion and the surrounding ligands; in the complexing reaction, the central metal ion and the ligand are combined through coordination bonds to form a complex; the coordination bond is formed by the overlap of the atomic orbital of the metal ion and the orbital of the lone pair of electrons or "electrons" on the ligand;

[0039] In complex reactions, ligands coordinate to central metal ions by donating one or more electron pairs; the central metal ions accept the electron pairs donated by the ligands to form stable complexes; the stability of the complex is usually related to the strength of the coordination bond between the metal ion and the ligand in the complex; the central metal ions in complex reactions are usually transition metal ions with variable valence; these metal ions have a higher degree of dissociation and a larger charge, which facilitates the formation of complexes with ligands; in complex reactions, ligands are substances that form coordination bonds with central metal ions. Ligands usually have lone pairs of electrons or elementary electrons and can form chemical bonds with central metal ions; common ligands include water molecules, ammonia molecules, halogen ions, carbonyl ligands, etc.; they form coordination bonds with central metal ions and stabilize the complexes by forming coordination bonds; the coordination number in complex reactions refers to the number of coordination bonds formed between the central metal ion and the ligand. The coordination number is usually related to the charge and coordinate number of the central metal ion;

[0040] Different central metal ions have different coordination numbers, such as divalent metal ions usually have a coordination number of 4 or 6, while trivalent metal ions usually have a coordination number of 6; complexation reactions usually occur within an appropriate range of H values; changes in H values ​​can affect the ionization state of the ligand in the solution, thereby affecting the progress of the complexation reaction; in some complexation reactions, changes in pH can cause dissociation or binding reactions of the ligand, thereby affecting the formation and stability of the complex; therefore, when conducting complexation reactions, the pH value of the solution needs to be controlled.

[0041] S4: The sterilized and enzymatically hydrolyzed liquid is subjected to amino acid testing, and other nutrients are added to form an organic liquid fertilizer containing amino acids.

[0042] In an embodiment of the present invention, the sterilized enzymatically hydrolyzed feed liquid is subjected to amino acid detection, and then other nutrients are added to form an organic liquid fertilizer containing amino acids. When the concentration of the amino acid detected is 10% higher than the national standard, the addition of other nutrients is stopped to form an organic liquid fertilizer containing amino acids.

[0043] S5: Perform antiseptic treatment on the amino acid-containing organic liquid fertilizer, package the amino acid-containing organic liquid fertilizer after the antiseptic treatment, store it in a cool place.

[0044] In the embodiment of the present invention, a plant-based preservative is added to the organic liquid fertilizer containing amino acids, and the addition amount is about 0.1%; the organic liquid fertilizer containing amino acids that has been subjected to preservative treatment and has met the national standard after testing is packaged and stored in a cool place, and the trace element composition and ingredient ratio of the amino acid-containing water-soluble fertilizer include: 50% pig blood, 15% composite amino acid powder, 0.002% Bacillus subtilis, 0.5% potassium dihydrogen phosphate, a fermentation additive, 2% zinc sulfate, 2% borax, and 30.498% water; the production equipment mainly includes:

[0045] Name of main production equipment Brand Model quantity Name of production unit Water Ring Vacuum Pump 5 Shandong Double Suction Pump Industry Co., Ltd. Pig Blood Bucket 1.5t capacity 2 Liquid pulverizer 1 Wenzhou Colloid Mill Factory Stainless steel fermentation tank 1.5t capacity 1 Zhengzhou Ruiheng Machinery Manufacturing Co., Ltd. Stainless steel mixing tank 1.5t2pcs, 3t1pc 3 Zhengzhou Ruiheng Machinery Manufacturing Co., Ltd. Stainless steel finished tank 1.5t capacity 1 Zhengzhou Ruiheng Machinery Manufacturing Co., Ltd. Heating cycle 1

[0046] Its main production process is: raw materials (pig blood, water) are added → polishing for 90 minutes → microbial strains are added (Bacillus subtilis) → put into fermentation barrel (heating) → temperature reaches 53°C → fermentation additives (potassium dihydrogen phosphate) are added for the first time → temperature reaches 58°C → fermentation additives (potassium dihydrogen phosphate) are added for the second time → fermentation for 72 hours → fine filtration → raw materials are added (water, zinc sulfate, borax, compound amino acid powder) → stirring and complexing for 40 minutes → inspection → metering and packaging → finished product.

[0047] The beneficial effects of the present invention are as follows: the present invention collects the blood of livestock and poultry in slaughterhouses in a centralized manner, then adds fermentation materials and ferments with Bacillus microorganisms, fully hydrolyzes the protein in the blood into amino acid form under specified conditions, and finally forms water-soluble fertilizer. The entire treatment process does not produce secondary treatment tailings, which is beneficial to solving the environmental pollution problem caused by the current discharge of waste blood. At the same time, the fertilizer produced can effectively increase crop yields. The present invention takes into account that the fishy smell of blood is heavy, which may easily lead to excessive odor in the finished fertilizer produced later, thereby causing discomfort to the user. The deodorization and deodorization operation is beneficial to removing the fishy smell in the liquid feed.

[0048] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood, characterized in that: The method comprises: S1: collecting blood from livestock and poultry in a slaughterhouse through a blood collection container, adding an anticoagulant to the blood from livestock and poultry in the slaughterhouse to obtain an anticoagulant raw material; S2: heating and disinfecting the anticoagulant raw material, adding Bacillus subtilis and fermentation additives for enzymolysis to obtain a hydrolyzed feed liquid; S3: filtering the enzymatically hydrolyzed liquid, performing a complexation treatment, and then performing an ultraviolet disinfection treatment to sterilize the enzymatically hydrolyzed liquid; S4: performing amino acid detection on the sterilized and enzymatically hydrolyzed feed liquid, and then adding other nutrients to form an organic liquid fertilizer containing amino acids; S5: performing an antiseptic treatment on the amino acid-containing organic liquid fertilizer, packaging the amino acid-containing organic liquid fertilizer after the antiseptic treatment, storing it in a cool place.

2. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: The livestock and poultry blood in step S1 is one or more of pig blood, sheep blood, cow blood and chicken and duck blood.

3. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: The ratio of the livestock and poultry blood to the anticoagulant in step S1 is 9:

1.

4. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: In step S1, anticoagulants are added to the blood of livestock and poultry in the slaughterhouse to obtain the mixing conditions of anticoagulant raw materials: anticoagulants are added to the blood of livestock and poultry in the slaughterhouse, and the blood is fully stirred and polished to perform anti-coagulation treatment.

5. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: The step of heating and disinfecting the anticoagulant raw material, adding Bacillus subtilis and a fermentation additive for enzymolysis to obtain an enzymolyzed feed liquid comprises: The anticoagulant raw material is heated to 53°C±1°C, disinfected, and then the Bacillus subtilis and potassium dihydrogen phosphate are added. When the temperature reaches 59°C±1°C, the potassium dihydrogen phosphate is added again and fermented for 72 hours to obtain a feed solution after enzymolysis.

6. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: In step S3, the complexing treatment process is: fermenting and filtering the enzymatically hydrolyzed liquid, adding the complexing composition therein, and performing complexing for 40 minutes.

7. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 6, characterized in that: The complex composition is 15% of imported composite amino acid powder, 2% of zinc sulfate and 2% of borax.

8. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 6, characterized in that: The complex composition comprises 30% of composite amino acid powder, 34-35% of zinc sulfate, 10-12% of borax and the balance of deionized water.

9. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: In step S4, the sterilized and enzymatically hydrolyzed liquid is subjected to amino acid detection, and other nutrients are added to form an organic liquid fertilizer containing amino acids. When the concentration of the amino acid detected is 10% higher than the national standard, the addition of other nutrients to form an organic liquid fertilizer containing amino acids is stopped.

10. The method for preparing amino acid-containing water-soluble fertilizer using slaughtered livestock and poultry blood according to claim 1, characterized in that: The step of subjecting the amino acid-containing organic liquid fertilizer to antiseptic treatment, packaging the amino acid-containing organic liquid fertilizer after antiseptic treatment, storing it in a cool place, comprises: Adding a plant-based preservative to the amino acid-containing organic liquid fertilizer in an amount of about 0.1%; After antiseptic treatment and testing to meet national standards, the amino acid-containing organic liquid fertilizer is packaged, stored and kept in a cool place.

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