Synergistic transdermal absorption promoting method
Through the synergistic effect of diol plastids, solid lipid nanoparticles and acylation chemical modification technology, the problem of insufficient transdermal absorption and stability of peptide active ingredients is solved, and the efficient and stable transdermality of peptide active ingredients is achieved. Absorption significantly improves the overall effect of skin care products.
Patent Information
- Application Number
- CN202411507327.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-28
- Publication Date
- 2025-01-14
AI Technical Summary
The transdermal absorption efficiency of peptide active ingredients in the skin environment is low and easily decomposed by enzymes, resulting in a decrease in effective concentration deep in the skin and affecting the skin care effect.
Using synergistic methods of diol plastids, solid lipid nanoparticles and acylation chemical modification technology, the penetration of peptide active ingredients is promoted through diol plastids, and solid lipid nanoparticles simulate cell membrane structure to protect peptide active ingredients , and improves its stability and skin-friendliness through acylation.
It significantly improves the transdermal absorption rate and stability of peptide active ingredients in the skin environment, ensuring that they can effectively penetrate the stratum corneum into the dermis, and enhance the overall efficacy of skin care products.
Smart Images
Figure CN119302856A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of skin care products, and more specifically, to a synergistic method for improving the transdermal absorption efficiency of skin care products mainly containing peptide active ingredients, and a preparation method and a use method thereof. The present invention is particularly suitable for applications where it is necessary to effectively deliver peptide active ingredients to the deep layers of the skin through the skin barrier, such as promoting the transdermal absorption of peptide active ingredients in anti-aging skin care products. Background Art
[0002] In recent years, as the public pays more and more attention to skin health and beauty, the skin care market has shown a booming trend, especially the growing demand for anti-aging functional skin care products. However, the effect of skin care products depends largely on whether the active ingredients can effectively penetrate the stratum corneum of the skin and enter the dermis. As the natural barrier of the skin, the stratum corneum poses a large penetration barrier to most active ingredients, which makes improving the transdermal absorption rate of active ingredients a key challenge in the research and development of skin care products. Especially for peptide active ingredients, this type of ingredient has attracted much attention due to its excellent efficacy in anti-aging and other aspects. However, the transdermal absorption efficiency of peptide active ingredients in the skin environment is relatively low, mainly because of its large molecular weight, which makes it difficult to effectively penetrate the stratum corneum of the skin. In addition, peptide active ingredients are easily decomposed by enzymes in the skin environment, resulting in a decrease in their effective concentration in the deep layer of the skin, thereby affecting the skin care effect.
[0003] The penetration-enhancing technologies currently available on the market mainly include the use of surfactants, solvents, and physical methods (such as microneedles, ultrasonic introduction, etc.). Although these methods can improve the transdermal absorption rate of active ingredients to a certain extent, they also have many shortcomings:
[0004] Surfactants and solvents: Although they can promote the penetration of active ingredients, they may cause skin irritation or dryness, and long-term use may also damage the skin barrier function;
[0005] Physical methods: Although technologies such as microneedles and ultrasonic introduction can significantly improve transdermal absorption efficiency, they are complex to operate, costly, and have certain risks of use, making them unsuitable for daily household use.
[0006] In addition, the stability of peptide active ingredients in the skin environment is also an important issue. Many peptide active ingredients are easily decomposed by enzymes in the skin environment, resulting in a decrease in their effective concentration deep in the skin, thus affecting the skin care effect.
[0007] In the market, single chemical modification methods, such as acetylation and palmitoylation, are often used for transdermal absorption of peptide ingredients. Although these methods can improve the stability of peptide ingredients to a certain extent, the following problems still exist:
[0008] Limitations of single chemical modification: Although single chemical modification can improve the stability of peptide components, it cannot significantly improve their transdermal absorption efficiency. Especially in complex skin environments, peptide components are still easily decomposed by enzymes, resulting in a decrease in their effective concentration in the dermis.
[0009] Limited local effect: Single chemical modification methods often only improve one aspect of peptide ingredients, and fail to take into account the complexity of skin absorption of active ingredients, resulting in limited effect in practical applications. The market urgently needs a new penetration-enhancing technology that can not only improve the transdermal absorption rate of peptide active ingredients, but also ensure their stability in the skin environment, so as to give full play to their efficacy.
[0010] In view of this, the present invention aims to provide a more systematic and comprehensive method for promoting the transdermal absorption of peptide active ingredients. By comprehensively using diol plasmids, solid lipid nanoparticles and acylation chemical modification technology, a synergistic system is formed to achieve efficient and stable transdermal absorption of peptide active ingredients from the stratum corneum to the dermis, thereby significantly improving the overall efficacy of skin care products. Summary of the invention
[0011] The present invention aims to provide a more systematic and comprehensive method for promoting the transdermal absorption of peptide active ingredients. By comprehensively using a variety of advanced technical means, the problems existing in the prior art are solved, the transdermal absorption efficiency of peptide active ingredients is improved, and at the same time, its stability in the skin environment is ensured, so as to give full play to its efficacy. Specifically, the present invention can improve the transdermal absorption rate of peptide active ingredients, so that they can effectively penetrate the stratum corneum of the skin and enter the dermis. The present invention can ensure the stability of peptide active ingredients in the skin environment, prevent them from being decomposed by enzymes, and maintain an effective concentration in the deep layers of the skin. The present invention can achieve efficient and stable transdermal absorption of peptide active ingredients, thereby significantly improving the overall efficacy of skin care products.
[0012] The technical solution of the present invention includes the following core components:
[0013] Diol plastids as penetration enhancers: A specific diol plastid is used as a penetration enhancer, wherein the diol plastid is selected from but not limited to propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, diethylene glycol or 1,3-butylene glycol, and the mass percentage of the diol plastid is 3% to 15% to enhance the penetration rate of the peptide active ingredient in the stratum corneum.
[0014] Diol plastids have unique amphiphilic properties that can effectively promote the diffusion of peptide active ingredients in the stratum corneum without causing irritation or damage to the skin.
[0015] Solid lipid nanoparticles (SLNs): including solid lipid nanoparticles (SLNs), the main component of which is selected from but not limited to hydrogenated lecithin or lecithin, and the particle size of the solid lipid nanoparticles is between 10 nanometers and 500 nanometers to ensure that it can effectively pass through the stratum corneum into the dermis. Solid lipid nanoparticles can simulate the cell membrane structure and interact with the stratum corneum, adjust the physical properties of the stratum corneum, thereby improving the permeability of peptide active ingredients and protecting peptide active ingredients from enzymatic hydrolysis.
[0016] Acylation chemical modification: The peptide active ingredient is a small molecule peptide, and the method further includes acylation chemical modification of the small molecule peptide to enhance its stability, skin affinity and bioavailability in the skin environment, and the acylation chemical modification includes but is not limited to acetyl, coconut oil acyl or palmitoyl.
[0017] Specifically, the acylation chemical modification is carried out using acylated peptide compounds, which include but are not limited to palmitoyl tripeptide, tetrapeptide, pentapeptide, hexapeptide, acetyl tripeptide, tetrapeptide, pentapeptide, hexapeptide, coconut oil tripeptide, tetrapeptide, pentapeptide, hexapeptide or other peptide compounds with similar structures, and the compounds have the function of improving skin conditions.
[0018] Acylation treatment not only improves the stability of peptide active ingredients in the skin environment, but also ensures their stable transmission in the presence of various enzymes and enhances their skin affinity and bioavailability.
[0019] Synergistic mechanism: The method includes using glycol plasmids, solid lipid nanoparticles and chemically modified peptide compounds in skin care products to ensure the synergistic effect of the three, thereby maximizing the transdermal absorption efficiency of the peptide active ingredient.
[0020] Through the synergistic mechanism, the ingredients promote each other to form a comprehensive transdermal absorption promotion system, achieving efficient and stable transdermal absorption of peptide active ingredients from the stratum corneum to the dermis.
[0021] Scope of application: The skin care products include but are not limited to lotions, creams, essences, etc., which are suitable for different types of skin and can be used in a variety of skin care scenarios.
[0022] Acylation chemical modification technology: The acylation chemical modification process includes but is not limited to N-terminal or C-terminal acylation to ensure that the peptide compound has higher stability in the skin enzyme environment and enhance its transdermal property, so that the peptide compound can maintain its activity in the dermis for a longer time.
[0023] The present invention forms a synergistic system by comprehensively utilizing diol plastids, solid lipid nanoparticles and acylation chemical modification technology, thereby achieving efficient and stable transdermal absorption of peptide active ingredients, significantly improving the overall efficacy of skin care products. Compared with the prior art, the present invention is unique in that it can solve the problem of insufficient transdermal absorption of peptide active ingredients and has broad application prospects.
[0024] Specifically, the technical solution of the present invention has the following advantages:
[0025] Efficient penetration: The use of glycol plasmids significantly increases the penetration rate of peptide active ingredients into the stratum corneum.
[0026] Enhanced stability: Acylation chemical modification technology significantly improves the stability of peptide active ingredients in the skin environment.
[0027] Comprehensive protection: The use of solid lipid nanoparticles provides additional protection, ensuring that peptide active ingredients can safely and effectively enter the dermis.
[0028] Synergistic effect: The three technical means work synergistically to form an efficient transdermal absorption promotion system, which significantly improves the transdermal absorption efficiency of peptide active ingredients. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 : Comparison of absorption rates of each experimental group at different time points. DETAILED DESCRIPTION
[0030] The present invention forms a synergistic system by comprehensively utilizing diol plastids, solid lipid nanoparticles (SLNs) and acylation chemical modification technology, thereby achieving efficient and stable transdermal absorption of peptide active ingredients from the stratum corneum to the dermis. The following is a detailed description of the specific implementation method:
[0031] Example 1: Preparation of a transdermal absorption enhancer containing diol plastids, acylated peptides and solid lipid nanoparticles (solution A)
[0032] 1) Preparation of unacylated peptide active ingredients: Raw material preparation: Tripeptide (such as Gly-Pro-Arg): as unacylated peptide active ingredient. Organic solvent (such as DMF): as solvent. Preparation steps: Dissolve the tripeptide in the organic solvent and stir evenly until completely dissolved.
[0033] 2) Palmitoylated peptide active ingredients: Raw material preparation: Tripeptide solution: as unacylated peptide active ingredient. Palmitic anhydride: as acylation reagent. Organic solvent (such as DMF): as solvent. Preparation steps: Add palmitic anhydride to the tripeptide solution and stir evenly. React for 2 hours under light-proof conditions to ensure that the acylation reaction is complete. After the reaction is completed, purify by high-performance liquid chromatography (HPLC) or solid phase extraction (SPE) to remove unreacted reagents. Use mass spectrometry (MS) technology to identify the purified product to ensure that the product is palmitoyl tripeptide.
[0034] 3) Preparation of solid lipid nanoparticles (SLNs): Raw material preparation: Hydrogenated lecithin: as the main component of solid lipid nanoparticles. Deionized water: as the solvent. Preparation steps: Dissolve hydrogenated lecithin in deionized water and homogenize it with a high-pressure homogenizer to prepare solid lipid nanoparticles with a particle size between 200 and 300 nanometers. Filter the obtained solid lipid nanoparticle suspension to remove large particle impurities.
[0035] 4) Encapsulation of acylated peptide active ingredients: Raw material preparation: Palmitoyl tripeptide: 2% by mass. Hydrogenated lecithin: as encapsulation material. Preparation steps: Dissolve palmitoyl tripeptide in deionized water, add hydrogenated lecithin, and disperse evenly by ultrasonic. Filter the resulting mixture to remove unencapsulated components to obtain solid lipid nanoparticles of acylated peptide active ingredients.
[0036] 5) Prepare a mixed solution: Raw material preparation: Propylene glycol: as the main component of the diol plastid, the mass percentage is 10%. Solid lipid nanoparticles of acylated peptide active ingredients: the mass percentages are 10%. Deionized water: as a solvent, make up to 100%. Preparation steps: Dissolve propylene glycol in deionized water and stir evenly until completely dissolved. Add the solid lipid nanoparticles of acylated peptide active ingredients to the above solution and stir evenly. Stir evenly and cool to room temperature.
[0037] Example 2: Preparation of a transdermal absorption enhancer (solution B) containing diol plastids, acylated peptides and solid lipid nanoparticles, similar to Example 1, except that the mass percentage of propylene glycol is 5%.
[0038] Example 3: Preparation of a transdermal absorption enhancer containing diol plastids, acylated peptides and solid lipid nanoparticles (Solution C)
[0039] 1) Preparation of unacylated peptide active ingredients: Raw material preparation: Tetrapeptide (such as Gly-Pro-Arg-Lys): as unacylated peptide active ingredient. Organic solvent (such as DMF): as solvent. Preparation steps: Dissolve the tetrapeptide in the organic solvent and stir evenly until completely dissolved.
[0040] 2) Acetylated peptide active ingredients: Raw material preparation: Tetrapeptide solution: as unacylated peptide active ingredient. Acetic anhydride: as acylation agent. Organic solvent (such as DMF): as solvent. Preparation steps: Add acetic anhydride to the tetrapeptide solution and stir evenly. React for 1 hour under light-proof conditions to ensure that the acylation reaction is complete. After the reaction is completed, purify by high performance liquid chromatography (HPLC) or solid phase extraction (SPE) to remove unreacted reagents. Use mass spectrometry (MS) technology to identify the purified product to ensure that the product is acetyl tetrapeptide.
[0041] 3) Preparation of solid lipid nanoparticles (SLNs): Raw material preparation: Hydrogenated lecithin: as the main component of solid lipid nanoparticles. Deionized water: as the solvent. Preparation steps: Dissolve hydrogenated lecithin in deionized water and homogenize it with a high-pressure homogenizer to prepare solid lipid nanoparticles with a particle size between 200 and 300 nanometers. Filter the obtained solid lipid nanoparticle suspension to remove large particle impurities.
[0042] 4) Encapsulation of acylated peptide active ingredients: Raw material preparation: Acetyl tetrapeptide: 2% by mass. Hydrogenated lecithin: as coating material. Preparation steps: Dissolve acetyl tetrapeptide in deionized water, add hydrogenated lecithin, and disperse evenly by ultrasonic. Filter the resulting mixture to remove unencapsulated components to obtain solid lipid nanoparticles of acylated peptide active ingredients.
[0043] 5) Prepare a mixed solution: Raw material preparation: Propylene glycol: as the main component of the diol plastid, the mass percentage is 10%. Solid lipid nanoparticles of acylated peptide active ingredients: the mass percentages are 10%. Deionized water: as a solvent, make up to 100%. Preparation steps: Dissolve propylene glycol in deionized water and stir evenly until completely dissolved. Add the solid lipid nanoparticles of acylated peptide active ingredients to the above solution and stir evenly. Cool to room temperature.
[0044] Example 4: Preparation of a transdermal absorption enhancer (solution D) containing glycol plastids, acylated peptides and solid lipid nanoparticles, similar to Example 3, except that the mass percentage of propylene glycol is 5%.
[0045] Comparative Example 1
[0046] Preparation of a transdermal absorption enhancer containing no glycol plasmids but containing acylated peptides and solid lipid nanoparticles (solution E)
[0047] 1) Preparation of unacylated peptide active ingredients: Raw material preparation: Tripeptide (such as Gly-Pro-Arg): as unacylated peptide active ingredient. Organic solvent (such as DMF): as solvent. Preparation steps: Dissolve the tripeptide in the organic solvent and stir evenly until completely dissolved.
[0048] 2) Palmitoylated peptide active ingredients: Raw material preparation: Tripeptide solution: as unacylated peptide active ingredient. Palmitic anhydride: as acylation reagent. Organic solvent (such as DMF): as solvent. Preparation steps: Add palmitic anhydride to the tripeptide solution and stir evenly. React for 2 hours under light-proof conditions to ensure that the acylation reaction is complete. After the reaction is completed, purify by high-performance liquid chromatography (HPLC) or solid phase extraction (SPE) to remove unreacted reagents. Use mass spectrometry (MS) technology to identify the purified product to ensure that the product is palmitoyl tripeptide.
[0049] 3) Preparation of solid lipid nanoparticles (SLNs): Raw material preparation: Hydrogenated lecithin: as the main component of solid lipid nanoparticles. Deionized water: as the solvent. Preparation steps: Dissolve hydrogenated lecithin in deionized water and homogenize it with a high-pressure homogenizer to prepare solid lipid nanoparticles with a particle size between 200 and 300 nanometers. Filter the obtained solid lipid nanoparticle suspension to remove large particle impurities.
[0050] 4) Encapsulation of acylated peptide active ingredients: Raw material preparation: Palmitoyl tripeptide: 2% by mass. Hydrogenated lecithin: as encapsulation material. Preparation steps: Dissolve palmitoyl tripeptide in deionized water, add hydrogenated lecithin, and disperse evenly by ultrasonic. Filter the resulting mixture to remove unencapsulated components to obtain solid lipid nanoparticles of acylated peptide active ingredients.
[0051] 5) Preparation of mixed solution: Raw material preparation: solid lipid nanoparticles containing acylated peptide active ingredients: the mass percentage is 10%.
[0052] Deionized water: as solvent, make up to 100%. Preparation steps: add solid lipid nanoparticles of acylated peptide active ingredients to the above solution, stir evenly. Stir evenly and cool to room temperature.
[0053] Comparative Example 2
[0054] Preparation of a transdermal absorption enhancer containing no diol plastids but containing acylated peptides but not encapsulated by solid lipid nanoparticles (Solution F)
[0055] 1) Preparation of unacylated peptide active ingredients: Raw material preparation: Tripeptide (such as Gly-Pro-Arg): as unacylated peptide active ingredient. Organic solvent (such as DMF): as solvent. Preparation steps: Dissolve the tripeptide in the organic solvent and stir evenly until completely dissolved.
[0056] 2) Palmitoylated peptide active ingredients: Raw material preparation: Tripeptide solution: as unacylated peptide active ingredient. Palmitic anhydride: as acylation reagent. Organic solvent (such as DMF): as solvent. Preparation steps: Add palmitic anhydride to the tripeptide solution and stir evenly. React for 2 hours under light-proof conditions to ensure that the acylation reaction is complete. After the reaction is completed, purify by high-performance liquid chromatography (HPLC) or solid phase extraction (SPE) to remove unreacted reagents. Use mass spectrometry (MS) technology to identify the purified product to ensure that the product is palmitoyl tripeptide.
[0057] 3) Acylated peptide active ingredients: Raw material preparation: Palmitoyl tripeptide: 2% by mass. Preparation steps: Dissolve palmitoyl tripeptide in deionized water and emulsify evenly through an emulsifying device to obtain an acylated peptide active ingredient solution.
[0058] 4) Prepare mixed solution: Raw material preparation: Solution of acylated peptide active ingredient: 10% by mass respectively. Deionized water: as solvent, make up to 100%. Stir evenly and cool to room temperature.
[0059] Comparative Example 3
[0060] Preparation of a transdermal absorption enhancer containing diol plastids, acylated peptides and but no solid lipid nanoparticles (solution G)
[0061] 1) Preparation of unacylated peptide active ingredients: Raw material preparation: Tripeptide (such as Gly-Pro-Arg): as unacylated peptide active ingredient. Organic solvent (such as DMF): as solvent. Preparation steps: Dissolve the tripeptide in the organic solvent and stir evenly until completely dissolved.
[0062] 2) Palmitoylated peptide active ingredients: Raw material preparation: Tripeptide solution: as unacylated peptide active ingredient. Palmitic anhydride: as acylation reagent. Organic solvent (such as DMF): as solvent. Preparation steps: Add palmitic anhydride to the tripeptide solution and stir evenly. React for 2 hours under light-proof conditions to ensure that the acylation reaction is complete. After the reaction is completed, purify by high-performance liquid chromatography (HPLC) or solid phase extraction (SPE) to remove unreacted reagents. Use mass spectrometry (MS) technology to identify the purified product to ensure that the product is palmitoyl tripeptide.
[0063] 3) Acylated peptide active ingredients: Raw material preparation: Take the palmitoyl tripeptide solution prepared in step 2: the mass percentage is 2%.
[0064] Preparation steps: dissolve palmitoyl tripeptide in deionized water and emulsify it evenly through an emulsifying device to obtain an acylated peptide active ingredient solution.
[0065] 4) Prepare mixed solution: Raw material preparation: Propylene glycol: as the main component of diol matrix, the mass percentage is 10%. Acylated peptide active ingredients: the mass percentage is 10%. Deionized water: as solvent, make up to 100%. Preparation steps: Dissolve propylene glycol in deionized water and stir evenly until completely dissolved. Add the acylated peptide active ingredients to the above solution and stir evenly. Stir evenly and cool to room temperature.
[0066] Experimental verification
[0067] Verify whether the transdermal absorption enhancer of the present invention can significantly improve the transdermal absorption rate of peptide active ingredients.
[0068] Subjects:
[0069] Three-dimensional reconstructed skin model: simulates human skin structure, including epidermis and dermis. Experimental group: transdermal absorption enhancers prepared in Examples 1 to 4 (solutions A, B, C, D). Control group: transdermal absorption enhancers prepared in Control Examples 1 to 3 (solutions E, F, G). Negative control group: a 2% non-chemically modified tripeptide solution was set as negative control group I for evaluation and comparison.
[0070] Experimental steps:
[0071] Model preparation: Prepare a three-dimensional reconstructed skin model to ensure that the model is in a good physiological state. Cut the skin model into a standard size of 1cm×1cm and clean it. Sample processing: Apply each group of solutions to the surface of the skin model to ensure that the application amount is consistent. Transdermal absorption detection: Fluorescence labeling method: Use fluorescently labeled peptide active ingredients (such as FITC-labeled peptides). After application, collect skin model samples at set time points (1 minute, 10 minutes, 1 hour). Use a fluorescence microscope to observe the distribution of peptide active ingredients in the skin and record the fluorescence intensity. Use image analysis software to quantitatively analyze the fluorescence intensity and calculate the transdermal absorption rate.
[0072] Experimental results:
[0073] Transdermal absorption rate: Calculate the transdermal absorption rate of each group of solutions at different time points (1 minute, 10 minutes, 1 hour) and perform statistical analysis. ANOVA (analysis of variance) was used to test the significant differences between the groups. The specific data are as follows:
[0074]
[0075] Experimental conclusion: Through experimental verification using a three-dimensional reconstructed skin model, it can be seen that the transdermal absorption rate of the transdermal absorption enhancer of the present invention is significantly higher than that of the control group at different time points. Specifically, at 1 minute, the absorption rate of the embodiment group is significantly higher than that of the control group and the negative control group, indicating that the technical solution of the present invention can significantly improve the transdermal absorption rate of peptide active ingredients in a short time.
[0076] The absorption rate of Example 3 (chemically modified with acetyl group) at 1 minute was 38.89%, which is higher than that of other example groups, showing its advantage in early absorption. At 10 minutes, the absorption rate of the example group increased further and was significantly higher than that of the control group, indicating that the technical solution of the present invention still maintains efficient transdermal absorption capacity in the medium term. The absorption rate of Example 3 at 10 minutes was 69.97%, which was significantly higher than that of other example groups, showing its advantage in medium-term absorption. At 1 hour, the absorption rate of the example group reached a peak value and was significantly higher than that of the control group, indicating that the technical solution of the present invention can still maintain a high transdermal absorption rate for a long time. The absorption rate of Example 3 at 1 hour was 86.25%, which was significantly higher than that of other example groups, showing its advantage in long-term absorption.
[0077] Statistical significance: The results of ANOVA (analysis of variance) test showed that the difference between the embodiment group and the control group was statistically significant (P<0.05), indicating that the technical solution of the present invention can significantly improve the transdermal absorption rate of peptide active ingredients.
[0078] In summary, the technical solution of the present invention can significantly improve the transdermal absorption rate of peptide active ingredients. This result verifies the effectiveness and superiority of the technical solution of the present invention, especially the method of chemical modification using acetyl group shows excellent transdermal absorption effect.
[0079] Although the above content fully describes and discloses the present invention through specific embodiments in detail, it should be understood that the present invention is not limited to these specific embodiments. After reading the present disclosure, those skilled in the art can make various reasonable changes, optimizations or equivalent replacements to the present invention in the spirit and scope of the appended claims for different application requirements and technical environments. All these improvements and modifications made based on the basic concept and innovative spirit of the present invention should also be regarded as falling within the scope of protection claimed by the present invention and are equally protected by law.
Claims
1. A synergistic method for promoting transdermal absorption, characterized in that: The method comprises a specific glycol plastid as a penetration enhancer, wherein the glycol plastid is selected from but not limited to propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, diethylene glycol or 1,3-butylene glycol, and the mass percentage of the glycol plastid is 3% to 15%, so as to enhance the penetration rate of the peptide active ingredient in the stratum corneum.
2. A synergistic transdermal absorption promoting method according to claim 1, characterized in that: It also includes solid lipid nanoparticles, the main component of which is selected from but not limited to hydrogenated lecithin or lecithin, and the particle size of the solid lipid nanoparticles is between 10 nanometers and 500 nanometers to ensure that it can effectively pass through the stratum corneum and enter the dermis.
3. The synergistic transdermal absorption enhancing method according to claim 1, characterized in that: The active ingredient is a small molecule peptide, and the method further comprises acylation chemical modification of the small molecule peptide to enhance its stability, skin affinity and bioavailability in the skin environment, wherein the acylation chemical modification includes but is not limited to acetyl or coconut oil acyl or palmitoyl.
4. A synergistic transdermal absorption enhancing method according to claim 3, characterized in that: The acylation chemical modification is carried out using acylated peptide compounds, which specifically include but are not limited to palmitoyl tripeptide, tetrapeptide, pentapeptide, hexapeptide, acetyl tripeptide, tetrapeptide, pentapeptide, hexapeptide, coconut oil tripeptide, tetrapeptide, pentapeptide, hexapeptide or other peptide compounds with similar structures, and the compounds have the function of improving skin conditions.
5. A synergistic transdermal absorption enhancing method according to any one of claims 1 to 4, characterized in that: The method comprises using glycol plasmids, solid lipid nanoparticles and chemically modified peptide compounds simultaneously in skin care products to ensure the synergistic effect of the three, thereby maximizing the transdermal absorption efficiency of the active ingredients.
6. A synergistic transdermal absorption enhancing method according to claim 5, characterized in that: The skin care product may be in the form of, but not limited to, lotion, cream or essence.
7. The synergistic transdermal absorption enhancing method according to claim 3, characterized in that: The acylation chemical modification process includes but is not limited to N-terminal or C-terminal acylation to ensure that the peptide compound has higher stability in the skin enzyme environment and enhance its transdermal property, so that the peptide compound can maintain its activity in the dermis for a longer time.
8. A synergistic transdermal absorption enhancing method according to any one of claims 1 to 7, characterized in that: The method forms a comprehensive transdermal absorption promotion system by combining diol plastids, solid lipid nanoparticles and acylation chemical modification technology, thereby achieving efficient and stable transdermal absorption of peptide active ingredients from the stratum corneum to the dermis, thereby significantly improving the overall efficacy of skin care products.