Temperature calibration method and device for different reaction plates

Abstract

The invention provides a temperature calibration method and device for different reaction plates, and the method comprises the steps: enabling a temperature sensor to pass through a through hole in a plug, enabling a lead of the temperature sensor to be tightly attached to the inner wall of a reaction hole in a spiral manner, enabling a temperature measurement head on the temperature sensor to be in contact with the bottom of the reaction hole, and enabling the temperature measurement head to be in contact with the bottom of the reaction hole; the upper part of the reaction hole is plugged by a plug, and an insulating layer is arranged outside the wire; putting the reaction plate of which the reaction holes are plugged with the temperature sensors into to-be-tested equipment, setting a heating program of the to-be-tested equipment, and starting to run; and transmitting and processing the real-time temperature signal of the bottom of the reaction hole by the temperature measuring head to obtain a real-time temperature value. The device can adapt to different reaction plates, and the temperature measurement accuracy can be improved.

Description

Technical Field

[0001] The invention relates to the field of measurement technology, and in particular to a temperature calibration method and device that can be used for different reaction plates. Background Art

[0002] Reaction plates are common consumables for biochemical instruments. There are also many types of equipment that use reaction plates, such as fluorescent reaction PCR instruments, nucleic acid extractors, constant temperature oscillators, coagulation analyzers, microplate readers and other experimental equipment or medical devices. Different manufacturers have different specifications and models. According to the number of reaction wells, they are divided into 96-well plates, 48-well plates, 16-well plates, etc.; according to the hole type, reaction plates have square holes, round holes, etc.; and the dimensions such as hole diameter, hole spacing, thickness, etc. used in different equipment are also different. For example, Thermo Fisher's 96-well plates have two specifications of 0.2mL and 0.1mL.

[0003] At present, when the equipment using reaction plates performs temperature measurement, each reaction well needs to be equipped with an independent temperature measurement unit. For example, the temperature calibrator of a PCR instrument can be used for temperature calibration of a fluorescence reaction PCR instrument, but cannot be used for temperature calibration of a nucleic acid extraction instrument. Summary of the invention

[0004] In order to solve the above problems in the prior art, the present invention provides a temperature calibration method and device that can be used for different reaction plates, which can adapt to the temperature calibration of all reaction holes.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a temperature calibration method that can be used for different reaction plates, comprising the steps of:

[0007] Pass the temperature sensor through the through hole on the plug, and place the wire of the temperature sensor in a spiral form close to the inner wall of the reaction hole, so that the temperature measuring head on the temperature sensor contacts the bottom of the reaction hole, and then plug the upper part of the reaction hole with the plug, and the wire has an insulating layer outside;

[0008] Putting a reaction plate with a temperature sensor plugged in a reaction hole into the device to be tested, setting a heating program for the device to be tested and starting the operation;

[0009] The temperature measuring head transmits and processes the real-time temperature signal at the bottom of the reaction hole to obtain a real-time temperature value.

[0010] The beneficial effects of the present invention are as follows: the wire is spirally attached to the inner wall of the reaction hole, which, on the one hand, adapts to the size of different reaction holes, thereby adapting to different reaction plates; on the other hand, the wire temperature of the temperature sensor is close to the temperature in the reaction hole through contact with the inner wall of the reaction hole, the temperature difference between the temperature measurement point and the adjacent wire is small, the heat exchange is also small, the influence of the wire heat conduction on the temperature measurement position is reduced, and the temperature measurement accuracy is improved. At the same time, the plug blocks the upper part of the reaction hole to reduce the heat flow, and the wire of the spring structure is in a compressed state, so that the temperature measuring head is close to the bottom of the reaction hole, the heat conduction efficiency is improved, and the temperature measurement accuracy is improved.

[0011] Optionally, the pitch of the wire of the temperature sensor at the lower part of the reaction hole is smaller than the pitch of the upper part.

[0012] According to the above description, the lower part of the temperature sensor wire is close to the temperature measurement point, which is more likely to cause heat exchange and affect the temperature measurement accuracy. Therefore, the number of turns is increased to make the lower part of the temperature sensor wire close to the temperature of the measurement point, thereby further improving the temperature measurement accuracy.

[0013] Optionally, the spiral diameter of the wire of the temperature sensor varies according to the shape of the reaction hole.

[0014] Optionally, the step of attaching the wire of the temperature sensor to the inner wall of the reaction hole in a spiral form comprises:

[0015] The wire of the temperature sensor is coiled into a spring structure in a spiral form, and then placed into the reaction hole so that it is closely attached to the inner wall of the reaction hole;

[0016] Alternatively, the wire of the temperature sensor is coiled in a spiral form on the inner wall of the reaction hole to form a spring structure.

[0017] Optionally, the step of transmitting and processing the real-time temperature signal of the bottom of the reaction well by the temperature measuring head to obtain the real-time temperature value comprises the following steps:

[0018] The temperature measuring head converts the real-time temperature signal of the bottom of the reaction hole into an electrical signal, transmits the electrical signal to the control unit, obtains the real-time temperature value, and transmits the real-time temperature value to the host computer.

[0019] Optionally, the method further comprises the steps of:

[0020] The real-time temperature value is displayed in real time, and the temperature of the device to be tested is calibrated according to the real-time temperature value.

[0021] In a second aspect, the present invention provides a temperature calibration device that can be used for different reaction plates, comprising a temperature measuring module and a plug, wherein the temperature measuring module comprises a temperature sensor, wherein the temperature sensor comprises a temperature measuring head and a wire, wherein the plug is a high temperature resistant elastic body and has a through hole, wherein the temperature sensor passes through the through hole;

[0022] The wire is used to be closely attached to the inner wall of the reaction hole in a spiral form, the temperature measuring head is used to contact the bottom of the reaction hole, and the plug is used to block the upper part of the reaction hole.

[0023] Optionally, the pitch of the wire of the temperature sensor at the lower part of the reaction hole is smaller than the pitch of the upper part.

[0024] Optionally, the spiral diameter of the wire of the temperature sensor varies according to the shape of the reaction hole.

[0025] Optionally, the temperature measurement module also includes a signal conversion module, a control unit, a power supply module and a remote transmission module, the power supply module is electrically connected to the temperature sensor, the signal conversion module, the control unit and the remote transmission module, and the control unit is electrically connected to the signal conversion module and the remote transmission module respectively.

[0026] Among them, the technical effect corresponding to the temperature calibration device that can be used for different reaction plates provided in the second aspect refers to the relevant description of the temperature calibration method that can be used for different reaction plates provided in the first aspect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A schematic flow chart of a temperature calibration method applicable to different reaction plates according to an embodiment of the present invention;

[0028] Figure 2 A schematic diagram of the coordination between the temperature sensor and the reaction hole involved in an embodiment of the present invention;

[0029] Figure 3 The figure is a schematic diagram of the cooperation between a temperature calibration device that can be used for different reaction plates and a host computer according to an embodiment of the present invention.

[0030] Description of reference numerals:

[0031] 1. Temperature sensor; 11. Wire; 12. Temperature measuring head;

[0032] 2. plug; 21. through hole;

[0033] 3. Signal conversion module;

[0034] 4. Control unit;

[0035] 5. Power module;

[0036] 6. Remote transmission module;

[0037] 100, host computer;

[0038] 200. Temperature measurement module;

[0039] 300. Reaction well. DETAILED DESCRIPTION

[0040] In order to better understand the above technical solution, exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a clearer and more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0041] Embodiment 1

[0042] In actual application scenarios, the reaction plate is placed in experimental equipment or medical equipment such as a fluorescent reaction PCR instrument, a nucleic acid extractor, a constant temperature oscillator, a coagulation analyzer, and an ELISA instrument. After the heating module of the experimental equipment is heated, a temperature field is formed in the reaction plate to provide temperature conditions for chemical and biological reactions in the reaction well. Therefore, temperature calibration in the reaction well is necessary. However, due to the different hole sizes and shapes of the reaction plate, there are many temperature measurement modules. Therefore, this embodiment solves the above problem by placing the wire in a spiral manner close to the inner wall of the reaction well.

[0043] Please refer to Figures 1 to 3 , this embodiment provides a temperature calibration method that can be used for different reaction plates, comprising the steps of:

[0044] S1. Pass the temperature sensor 1 through the through hole 21 on the plug 2, and spirally place the wire 11 of the temperature sensor 1 close to the inner wall of the reaction hole 300, and make the temperature measuring head 12 on the temperature sensor 1 contact the bottom of the reaction hole 300. Then, block the upper part of the reaction hole 300 with the plug 2, and the wire 11 has an insulating layer on the outside.

[0045] In this embodiment, the stopper 2 is a high temperature resistant elastomer, such as PVC plastic, silicone, etc., which can be adapted to the upper part of the reaction hole 300 of different sizes to block the upper part of the reaction hole 300 and reduce heat flow.

[0046] The through hole 21 is arranged in the middle area of ​​the plug 2 .

[0047] The wire 11 has an insulating layer outside, which avoids the short circuit problem caused by the close contact of the wire 11.

[0048] Thus, the temperature measuring head 12 of the temperature sensor 1 contacts the bottom of the reaction hole 300 to measure the bottom temperature of the reaction hole 300. However, since the sensor wire 11 has thermal conductivity, if the wire 11 is pulled out of the reaction hole 300 in a straight line, due to the large temperature difference between the inside of the reaction hole 300 and the environment, for example, 90°C in the reaction hole 300 and 23°C in the environment, the temperature difference between the two ends of the wire 11 is large, and the metal conducts heat quickly, which has a great impact on the temperature field of the temperature measuring point, and the temperature at the temperature measuring point cannot be accurately measured. Therefore, in this embodiment, the wire 11 of the spring structure is made to contact the inner wall of the reaction hole 300 as much as possible, so that the temperature of the wire 11 of the temperature sensor 1 is close to the temperature inside the reaction hole 300, the temperature difference between the temperature measuring point and the adjacent wire 11 is small, and the heat exchange is also small, which reduces the influence of the heat conduction of the wire 11 on the temperature measuring position and improves the temperature measurement accuracy. In addition, the spring structure produces elastic deformation, and the extrusion caused by the plug 2 fixing the upper part of the reaction hole 300 makes the temperature measuring point of the temperature sensor 1 close to the bottom of the reaction hole 300, thereby improving the heat conduction efficiency.

[0049] Specifically, in step S1 of this embodiment, the step of attaching the wire 11 of the temperature sensor 1 to the inner wall of the reaction hole 300 in a spiral form includes:

[0050] The wire 11 of the temperature sensor 1 is coiled into a spring structure in a spiral form, and then placed into the reaction hole 300 so that it is closely attached to the inner wall of the reaction hole 300;

[0051] Alternatively, the wire 11 of the temperature sensor 1 is coiled in a spiral form on the inner wall of the reaction hole 300 to form a spring structure.

[0052] The wire 11 is first coiled into a spring structure and then placed into the reaction hole 300 , and can be heated and fixed if necessary.

[0053] In this embodiment, the pitch of the wire 11 of the temperature sensor 1 at the lower part of the reaction hole 300 is smaller than the pitch of the upper part. This is because the lower part of the wire 11 of the temperature sensor 1 is close to the temperature measurement point, which is more likely to cause heat exchange and affect the temperature measurement accuracy. Therefore, the number of turns is increased to make the lower part of the wire 11 of the temperature sensor 1 close to the temperature of the measurement point, thereby further improving the temperature measurement accuracy.

[0054] In this embodiment, the spiral diameter of the wire 11 of the temperature sensor 1 varies according to the shape of the reaction hole. Since the current reaction hole 300 is basically a cone, the bottom diameter is small, and the diameter gradually increases from the bottom to the top, so the spiral diameter of the wire 11 also gradually increases, but the corresponding spiral diameter will change with different reaction hole 300 shapes.

[0055] S2. Place the reaction plate with the temperature sensor 1 plugged in the reaction hole 300 into the device to be tested, set the heating program of the device to be tested and start the operation.

[0056] The equipment to be tested includes a PCR instrument, a nucleic acid extractor, etc. After setting and running the heating program of the equipment to be tested, the temperature in the reaction hole 300 will begin to change gradually.

[0057] S3. The temperature measuring head 12 transmits and processes the real-time temperature signal of the bottom of the reaction hole 300 to obtain a real-time temperature value.

[0058] The temperature measuring head 12 transmits and processes the real-time temperature signal at the bottom of the reaction hole 300 to obtain the real-time temperature value, including the following steps:

[0059] The temperature measuring head 12 converts the real-time temperature signal at the bottom of the reaction hole 300 into an electrical signal, transmits the electrical signal to the control unit 4, obtains the real-time temperature value, and transmits the real-time temperature value to the host computer 100.

[0060] Among them, signal conversion includes AD conversion, signal amplification and so on.

[0061] S4. Display the real-time temperature value in real time, and perform temperature calibration on the device to be tested according to the real-time temperature value.

[0062] The host computer 100 receives and displays the real-time temperature value, thereby realizing temperature calibration of the device to be tested.

[0063] Embodiment 2

[0064] Please refer to Figures 1 to 3 The present embodiment provides a temperature calibration device that can be used for different reaction plates, including a temperature measuring module 200 and a plug 2. The temperature measuring module 200 includes a temperature sensor 1. The temperature sensor 1 includes a temperature measuring head 12 and a wire 11. The plug 2 is a high-temperature resistant elastomer and is provided with a through hole 21. The temperature sensor 1 passes through the through hole 21. The wire 11 is used to be tightly attached to the inner wall of the reaction hole 300 in a spiral form, the temperature measuring head 12 is used to contact the bottom of the reaction hole 300, and the plug 2 is used to block the upper part of the reaction hole 300.

[0065] In this embodiment, the pitch of the wire 11 of the temperature sensor 1 at the bottom of the reaction hole 300 is smaller than that at the top to improve the temperature measurement accuracy. The spiral diameter of the wire 11 of the temperature sensor 1 varies according to the shape of the reaction hole to adapt to most existing reaction holes 300.

[0066] The temperature measuring head 12 of the temperature sensor 1 is a thermocouple or a platinum resistor.

[0067] Reference Figure 3It can be seen that the temperature measurement module 200 also includes a signal conversion module 3, a control unit 4, a power module 5 and a remote transmission module 6. The power module 5 is electrically connected to the temperature sensor 1, the signal conversion module 3, the control unit 4 and the remote transmission module 6. The control unit 4 is electrically connected to the signal conversion module 3 and the remote transmission module 6. Among them, the power module 5 supplies power to the other modules as a whole, and the figure only illustrates the control connection of the control unit 4 to the power module 5.

[0068] On this basis, the remote transmission module 6 in this embodiment is a Wi-Fi module.

[0069] Therefore, the working process of the host computer 100 of this embodiment is also described as follows:

[0070] 1. Basic information management, including information about the commissioning unit and sample information. Users need to create a test project that complies with the national PCR calibration procedures, fill in the commissioning unit information, and relevant information of the sample to be tested, including unit name, contact person, model specifications, etc.

[0071] 2. Verification process management is achieved through real-time data collection. The system uses Wi-Fi to communicate with the temperature measurement module 200, and the sampling frequency can reach 100 milliseconds. The collected data is displayed in the form of a real-time curve, which can intuitively understand the entire temperature change process. It is also displayed in the form of a data list, so that the actual temperature values ​​of each time period collected can be directly viewed.

[0072] 3. Data processing, including data correction and data calculation. The correction value of the temperature sensor can be obtained through calibration, and the correction can be made in real time after entering the system. Each time the calibration is completed, the average value, indication error, uncertainty, uniformity, average heating rate, average cooling rate and test conclusion of the test data are automatically calculated.

[0073] 4. System management, including generating original records, user management, historical data query and system parameter setting. After calibration, it is necessary to generate original records that comply with the regulations. The original records include the information of the inspection unit, PCR instrument related information and test data. User management can add, delete users and modify user information. Historical data query can query the test items that have been done in the past. You can query the required test item information by date, unit information, sample information and other related information, and generate original records. System parameter settings include information such as standard instrument and calibration personnel settings.

[0074] Therefore, this embodiment is adapted to different reaction plates and improves the temperature measurement accuracy by designing the coordination between the temperature sensor 1 and the reaction hole 300 .

[0075] Therefore, it should be noted that the specific selection of each module in this embodiment is a specific example. In other equivalent embodiments, models that can meet the corresponding functions can be used for replacement.

[0076] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0077] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0078] In the present invention, unless otherwise clearly specified and limited, when a first feature is “on” or “below” a second feature, it may be that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, when a first feature is “above”, “above” or “above” a second feature, it may be that the first feature is directly above or obliquely above the second feature, or it may simply mean that the first feature is higher in level than the second feature. When a first feature is “below”, “below” or “below” a second feature, it may be that the first feature is directly below or obliquely below the second feature, or it may simply mean that the first feature is lower in level than the second feature.

[0079] In the description of this specification, the description of the terms "one embodiment", "some embodiments", "embodiment", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0080] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may alter, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A temperature calibration method that can be used for different reaction plates, characterized in that: Includes steps: Pass the temperature sensor through the through hole on the plug, and place the wire of the temperature sensor in a spiral form close to the inner wall of the reaction hole, so that the temperature measuring head on the temperature sensor contacts the bottom of the reaction hole, and then plug the upper part of the reaction hole with the plug, and the wire has an insulating layer outside; Putting a reaction plate with a temperature sensor plugged in a reaction hole into the device to be tested, setting a heating program for the device to be tested and starting the operation; The temperature measuring head transmits and processes the real-time temperature signal at the bottom of the reaction hole to obtain a real-time temperature value.

2. A temperature calibration method for different reaction plates according to claim 1, characterized in that: The pitch of the wire of the temperature sensor at the lower part of the reaction hole is smaller than the pitch of the upper part.

3. A temperature calibration method for different reaction plates according to claim 1, characterized in that: The spiral diameter of the wire of the temperature sensor varies according to the shape of the reaction hole.

4. A temperature calibration method applicable to different reaction plates according to claim 1, characterized in that: The step of attaching the wire of the temperature sensor to the inner wall of the reaction hole in a spiral form comprises: The wire of the temperature sensor is coiled into a spring structure in a spiral form, and then placed into the reaction hole so that it is closely attached to the inner wall of the reaction hole; Alternatively, the wire of the temperature sensor is coiled in a spiral form on the inner wall of the reaction hole to form a spring structure.

5. A temperature calibration method for different reaction plates according to any one of claims 1 to 4, characterized in that: The step of transmitting and processing the real-time temperature signal of the bottom of the reaction hole by the temperature measuring head to obtain the real-time temperature value comprises the following steps: The temperature measuring head converts the real-time temperature signal of the bottom of the reaction hole into an electrical signal, transmits the electrical signal to the control unit, obtains the real-time temperature value, and transmits the real-time temperature value to the host computer.

6. A temperature calibration method for different reaction plates according to any one of claims 1 to 4, characterized in that: Also includes the steps: The real-time temperature value is displayed in real time, and the temperature of the device to be tested is calibrated according to the real-time temperature value.

7. A temperature calibration device that can be used for different reaction plates, characterized in that: It comprises a temperature measuring module and a plug, wherein the temperature measuring module comprises a temperature sensor, the temperature sensor comprises a temperature measuring head and a wire, the plug is a high temperature resistant elastic body and is provided with a through hole, and the temperature sensor passes through the through hole; The wire is used to be closely attached to the inner wall of the reaction hole in a spiral form, the temperature measuring head is used to contact the bottom of the reaction hole, and the plug is used to block the upper part of the reaction hole.

8. A temperature calibration device that can be used for different reaction plates according to claim 7, characterized in that: The pitch of the wire of the temperature sensor at the lower part of the reaction hole is smaller than the pitch of the upper part.

9. A temperature calibration device that can be used for different reaction plates according to claim 7, characterized in that: The spiral diameter of the wire of the temperature sensor varies according to the shape of the reaction hole.

10. A temperature calibration device that can be used for different reaction plates according to claim 7, characterized in that: The temperature measurement module also includes a signal conversion module, a control unit, a power module and a remote transmission module. The power module is electrically connected to the temperature sensor, the signal conversion module, the control unit and the remote transmission module. The control unit is electrically connected to the signal conversion module and the remote transmission module respectively.

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