Formula for resolving dampness and reducing fat and application thereof

Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a dampness-resolving fat-reducing formula and application thereof. The dampness-resolving and fat-reducing formula comprises the following main active components in parts by mass: 10-15 parts of radix bupleuri, 5-15 parts of radix curcumae, 10-15 parts of cortex magnoliae officinalis, 5-15 parts of nutmeg, 10-30 parts of coix seeds, 3-10 parts of apricot kernels, 10-15 parts of rhizoma atractylodis and 5-15 parts of dried orange peel. Animal experiments show that the dampness-resolving and fat-reducing formula has the effect of improving the weight of mice with dyslipidemia; the liver lesion degree of a mouse with lipid metabolism disorder can be relieved, the liver cell structure is improved, and the number of lipids and lipid droplets is reduced; the lipid metabolism disorder level of mice induced by high fat diet can be improved; a certain improvement effect is achieved on the liver function of a mouse with lipid metabolism disorder. Therefore, the dampness-resolving and fat-reducing formula disclosed by the invention has a good treatment effect on dyslipidemia.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and particularly relates to a prescription for eliminating dampness and reducing fat and application thereof. Background Art

[0002] Lipid metabolism disorder is a disease caused by abnormal fat metabolism or transport, which causes the cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) in plasma to exceed the normal range and (or) the high-density lipoprotein cholesterol (HDL-C) level to be lower than the normal range. It is closely related to many diseases such as obesity, type 2 diabetes, metabolic-related fatty liver disease, hypertension, coronary heart disease, stroke, etc., and seriously endangers human health. Surveys show that the prevalence of lipid metabolism disorders in adults in my country is as high as 30%. At present, the commonly used lipid-lowering drugs in Western medicine are statins, fibrates, niacin, bile acid chelating resins, cholesterol absorption inhibitors, etc. However, these drugs have high hepatotoxicity, the treatment effect is not ideal, and some drugs are expensive, so they are rejected by patients.

[0003] Traditional Chinese medicine has gradually attracted attention and importance in the treatment of lipid metabolism disorders due to its multi-target effect and small side effects. At present, there is still a lack of traditional Chinese medicine compositions that can effectively treat lipid metabolism disorders in clinical practice, so it is necessary to develop a traditional Chinese medicine composition that can effectively treat lipid metabolism disorders. Summary of the invention

[0004] The first object of the present invention is to provide a prescription for removing dampness and reducing fat. The second object of the present invention is to provide an application of the prescription for removing dampness and reducing fat.

[0005] According to a first aspect of the present invention, a prescription for eliminating dampness and reducing fat is provided, wherein the main active ingredients thereof include, by weight, 10-15 parts of bupleurum, 5-15 parts of curcuma, 10-15 parts of magnolia bark, 5-15 parts of cardamom, 10-30 parts of coix seed, 3-10 parts of apricot kernel, 10-15 parts of atractylodes, and 5-15 parts of tangerine peel.

[0006] The present invention believes that obesity, hyperlipidemia and other lipid metabolism disorders generally belong to the categories of "phlegm turbidity", "blood stasis", "wet turbidity", etc., and are closely related to the liver, spleen, kidney, triple energizer and other internal organs. The liver is responsible for dredging and dispersing, regulating the qi mechanism, allowing the normal operation of qi and blood, and can also promote bile excretion and transport the middle energizer water and grain; the liver fails to dredge and discharge, and the spleen and soil are blocked, the spleen fails to transport, the bile excretion is unfavorable, the phlegm turbidity is endogenous, the cream blocks the meridians, and the qi and blood are stagnant. The spleen is responsible for transportation and transformation, and the essence of water and grain depends on the spleen to rise the clear and lower the turbidity, and the whole body is covered; the spleen fails to transport, the body fluids stop and gather, which is phlegm and wet, and the cream biochemical disorder is abnormal, and it is located in one place, causing illness. The kidney yang is vigorous and warms the body, which is conducive to the gasification and application of the cream; the kidney yin is sufficient, nourishing the limbs and bones, which is conducive to the storage of the cream; the kidney essence is weak, the yang has nothing to grow, and the yin has nothing to transform, then the dredge and discharge are delinquent, and the primary disease is caused. Phlegm and turbidity block the blood flow for a long time, causing blood stasis. After a long time, phlegm and stasis will accumulate in the blood vessels and the meridians will lose their flexibility. The dysfunction of triple energizer qi transformation is an important pathogenesis of triple energizer diseases such as lipid metabolism disorder. Lipid metabolism disorder is treated by "treating from the liver", "treating from the spleen", "treating from the kidney", "treating from phlegm" and "treating from stasis". These are all individual emphases on a certain pathogenesis of the dysfunction of triple energizer qi transformation. The dysfunction of triple energizer qi transformation is a more comprehensive summary of its pathological mechanism. The triple energizer is the main hub and main pipeline of human metabolism. If the triple energizer is dysfunctional, the "pipeline is blocked" or is unblocked, the upper energizer cannot spread the fine qi, the middle energizer cannot rot water and grain, and the lower energizer cannot excrete the body's waste, leading to metabolic disorders. Lipid metabolism disorders are mostly caused by overeating fat and sweet food, which exceeds the transportation and transformation capacity of the triple energizer, or the triple energizer's transmission disorder, which causes the water and grain essence to be unable to be used by the body in the right way, but accumulates in the body, causing phlegm and turbidity, and filling the triple energizer, blocking the channel, stagnating qi and blood, and endangering the whole body. In short, the present invention believes that the dysfunction of triple energizer gasification is an important pathogenesis of triple energizer diseases such as lipid metabolism disorders; the method of unblocking the triple energizer is the main treatment method for triple energizer diseases such as lipid metabolism disorders. The dampness-removing and fat-reducing prescription of the present invention uses unblocking the triple energizer as a method, and simultaneously promotes the lung to reduce adverse reactions, dries dampness and strengthens the spleen, and relieves liver depression, regulates the functions of the upper, middle and lower energizer organs, and achieves the purpose of treating diseases related to lipid metabolism disorders.

[0007] This prescription uses Bupleurum and Curcuma as the main drugs, Magnolia officinalis, Cardamom, Coix seed, and Apricot kernel as the assistant drugs, Atractylodes lancea as the adjuvant drug, and Tangerine peel as the guiding drug. Bupleurum soothes the liver and relieves depression, regulates the qi, and can promote bile excretion, qi and blood circulation, and fat metabolism; Curcuma invigorates blood circulation, promotes qi and relieves depression, and cooperates with Bupleurum to strengthen the function of soothing the liver and relieving depression, improving qi and blood stagnation; the two work together to promote the normal metabolism of qi, blood, and fat by soothing the liver and relieving depression. Magnolia officinalis dries dampness and resolves phlegm, promotes qi and relieves fullness, and assists Bupleurum and Curcuma to strengthen the function of soothing the liver and regulating qi. Apricot kernel promotes the qi of the upper Jiao lung, and the qi moves and the dampness is transformed; Cardamom moves qi and removes dampness to promote the spleen in the middle Jiao; Coix seed penetrates dampness and strengthens the spleen, so that damp heat is removed from the lower Jiao. Apricot kernels promote upward movement, cardamom seeds promote middle movement, and coix seeds penetrate downward, regulating the three energizers. When used together, they can help dampness and heat to be eliminated from the three energizers. This means that the upper gate is opened, the branch river is opened, and the water is guided downward. This can help qi to flow and dampness to flow, spleen function to recover, and the three energizers to flow smoothly. Atractylodes lancea can dry out dampness and strengthen the spleen, and is an adjuvant drug. It assists the dampness-removing drugs in the ministerial drugs, strengthens the dampness-removing effect, and at the same time restricts the dredging effect of the monarch drug to prevent excessive dredging. Tangerine peel regulates qi and regulates the middle, dries dampness and resolves phlegm, and is an adjuvant drug. It guides all the drugs to the lesion site directly, and plays a role in harmonizing all the drugs, so that they work together to eliminate evil. All the drugs work together to promote the three energizers, soothe the liver and relieve depression, dry dampness and strengthen the spleen, and promote the lungs and reduce adverse effects. By regulating the functions of the upper, middle, and lower energizer organs, it promotes the circulation of qi and blood and the metabolism of fat, and achieves the purpose of improving lipid metabolism disorders. This reflects the holistic concept and syndrome differentiation principle of traditional Chinese medicine in treating diseases, and restores the body's normal metabolic function through multi-link and multi-target regulation.

[0008] The sources of raw materials for this recipe are as follows:

[0009] Bupleurum: The dried root of Bupleurum chinensie DC. or Bupleurumscorzonerifolium Willd. of the Apiaceae family.

[0010] Curcuma: The dried tubers of Curcuma wenyujin YHChen et C.Ling, Curcuma longa L., Curcuma kwangsiensis SGLee et C.F.Liang or Curcuma phaeocaulis Val. of the Zingiberaceae family.

[0011] Magnolia officinalis: the dried bark, root bark and branch bark of Magnolia officinalis Rehd.et Wils. or Magnolia offinalis Rehd.et Wils.var.biloba Rehd.et Wils., a plant of the genus Magnolia in the Magnoliaceae family.

[0012] Cardamom: The ripe fruit of white cardamom and Java white cardamom, both of the ginger family.

[0013] Job's tears seed: the dried mature seed kernel of Coix lacryma jobi L. var. mayuen (Roman.) Stapf, a plant of the genus Coix of the Poaceae family.

[0014] Almond: seeds of Armeniaca vulgaris Lam. [Prunus armeniaca L.], wild apricot A.vulgaris Lam.var.ansu(Maxim)Yu et Lu [Prunus armeniaca L.var.ansu Maxim.], mountain apricot A.sibirica(L.)Lam. [Prunus sibirica L.], and northeastern apricot A.mandshurica(Maxim.)Skv. [Prunus mandshurica(Maxim.)Koehne] of the genus Apricot in the Rosaceae family.

[0015] Atractylodes: The dried rhizome of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz., plants of the genus Atractylodes in the Asteraceae family.

[0016] Tangerine peel: the dried mature peel of Citrus reticulata Blanco and its cultivated varieties.

[0017] In some embodiments, the main active ingredients include, by mass, 12 parts of bupleurum, 10 parts of curcuma, 12 parts of magnolia bark, 10 parts of cardamom, 20 parts of coix seed, 6 parts of apricot, 12 parts of atractylodes, and 10 parts of tangerine peel.

[0018] In some embodiments, pharmaceutically acceptable adjuvants are also included. Specifically, the adjuvants used are carriers or excipients commonly used in the art, including but not limited to starch, lactose, glucose, sodium carboxymethyl cellulose, ethyl cellulose and methyl cellulose, malt, gelatin, polyols (such as propylene glycol, glycerol, mannitol), compressed tablets, etc., to help improve the stability or activity of the drug, or produce an acceptable taste or smell in the case of oral administration.

[0019] In some embodiments, the dosage form of the Huashi Jianzhi prescription is decoction, oral solution, granules, powder, tablet, pill or capsule, so as to facilitate clinical administration.

[0020] According to a second aspect of the present invention, there is provided use of the above-mentioned formula for removing dampness and reducing fat in the preparation of a medicine for treating lipid metabolism disorders.

[0021] According to a third aspect of the present invention, there is provided use of the above-mentioned formula for removing dampness and reducing fat in the preparation of a medicament for treating metabolism-related fatty liver disease.

[0022] According to a fourth aspect of the present invention, there is provided use of the above-mentioned formula for removing dampness and reducing fat in the preparation of a drug for treating dyslipidemia and / or obesity and / or fatty liver.

[0023] The beneficial effects of the present invention include:

[0024] (1) The present invention is based on the clinical pathogenesis of TCM of "liver failure to dredge and release, qi stagnation, and damp-heat accumulation" in patients with metabolic-related fatty liver, takes the method of unblocking the three energizers as the method, and simultaneously promotes the lungs to reduce adverse effects, dries dampness and strengthens the spleen, soothes the liver and relieves depression, regulates the functions of the upper, middle and lower energizer organs, and creates a prescription for eliminating dampness and reducing fat. The prescription is clinically used to treat dyslipidemia, obesity, and fatty liver, and has a definite efficacy and good safety.

[0025] (2) Animal experiments show that the Huashi Jianzhi recipe of the present invention has the effect of improving the body weight of mice with lipid metabolism disorders; can reduce the degree of liver lesions in mice with lipid metabolism disorders, improve the liver cell structure, and reduce the number of lipids and lipid droplets; can improve the level of lipid metabolism disorders in mice induced by a high-fat diet; and has a certain improvement effect on the liver function of mice with lipid metabolism disorders. This suggests that the Huashi Jianzhi recipe of the present invention has a good therapeutic effect on lipid metabolism disorders. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a weight growth curve diagram of each group of mice in the animal experiment of the present invention.

[0027] Figure 2 This is a graph showing the final body weight of each group of mice in the animal experiment of the present invention.

[0028] Figure 3 This is the HE staining result of the liver of each group in the animal experiment of the present invention.

[0029] Figure 4 This is the Oil Red O staining result of the liver of each group in the animal experiment of the present invention.

[0030] Figure 5 It is the measurement result of serum lipid level of each group of mice in the animal experiment of the present invention.

[0031] Figure 6 It is the measurement result of the liver function index of each group of mice in the animal experiment of the present invention. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below in conjunction with specific examples. Unless otherwise specified, the raw materials and reagents used in the present invention are all purchased from the market.

[0033] Example 1

[0034] The dampness-removing and fat-reducing Chinese herbal decoction of the present embodiment is prepared from the following raw materials: 15g of bupleurum, 10g of turmeric, 15g of magnolia bark, 15g of cardamom seed, 20g of coix seed, 10g of apricot kernel, 15g of atractylodes, and 15g of tangerine peel.

[0035] The above components are taken and a Chinese medicine decoction is prepared according to an existing Chinese medicine decoction preparation method. The ratio is one dose of medicine, and one dose of medicine is about 300 mL.

[0036] Exemplarily, the preparation method of the Chinese herbal decoction is specifically as follows: mix the above-mentioned Chinese medicinal materials according to a ratio to obtain a mixture, then add it into water at a solid-liquid ratio of 1:10, soak it for 0.5h, heat it to boiling over high heat, simmer it over low heat for 1h, filter it to obtain a first filtrate and a residue; add water to the residue at a solid-liquid ratio of 1:8, heat it to boiling over high heat, simmer it over low heat for 1h, filter the obtained filtrate, combine it with the first filtrate, sterilize it, cool it to room temperature, and then package it.

[0037] Example 2

[0038] The dampness-removing and fat-reducing Chinese herbal decoction of the present embodiment is prepared from the following raw materials: 12g of bupleurum, 10g of turmeric, 12g of magnolia bark, 10g of cardamom, 20g of coix seed, 6g of apricot, 12g of atractylodes, and 10g of tangerine peel.

[0039] The preparation method is the same as that of Example 1.

[0040] Example 3

[0041] The dampness-removing and fat-reducing Chinese herbal decoction of the present embodiment is prepared from the following raw materials: 15g of bupleurum, 15g of turmeric, 15g of magnolia bark, 15g of cardamom seed, 30g of coix seed, 10g of apricot kernel, 15g of atractylodes, and 15g of tangerine peel.

[0042] The preparation method is the same as that of Example 1.

[0043] Example 4

[0044] The dampness-removing and fat-reducing Chinese herbal decoction of this embodiment is prepared from the following raw materials: 10g of bupleurum, 5g of turmeric, 10g of magnolia bark, 5g of cardamom, 10g of coix seed, 3g of apricot kernel, 10g of atractylodes, and 5g of tangerine peel.

[0045] The preparation method is the same as that of Example 1.

[0046] Next, to illustrate the therapeutic effect of the Huashi Jianzhi prescription of the present invention on lipid metabolism disorders, the Chinese medicine decoction prepared in Example 1 was concentrated and then subjected to the following animal experiment to evaluate its therapeutic effect. It should be noted that the drug concentration used in animal experiments is different from that used in humans, and the drug concentration used in mice is about 9 times that of humans. Therefore, the Chinese medicine decoction prepared in Example 1 was concentrated and then administered to mice.

[0047] 1. Experimental Materials

[0048] 1. Experimental reagents

[0049] The main reagents used in the experiment are shown in Table 1.

[0050] Table 1 Main reagents used in the experiment

[0051] Reagent name Manufacturer Total Cholesterol (TC) Assay Kit Nanjing Jiancheng Bioengineering Institute Triglyceride (TG) Assay Kit Nanjing Jiancheng Bioengineering Institute High-density lipoprotein cholesterol (HDL-C) test kit Nanjing Jiancheng Bioengineering Institute Low-density lipoprotein cholesterol (LDL-C) test kit Nanjing Jiancheng Bioengineering Institute Total Superoxide Dismutase (SOD) Assay Kit Nanjing Jiancheng Bioengineering Institute Total Bile Acid (TBA) Kit Nanjing Jiancheng Bioengineering Institute Malondialdehyde (MDA) Assay Kit Nanjing Jiancheng Bioengineering Institute 4% paraformaldehyde universal tissue fixative White Shark Biotechnology Co., Ltd.

[0052] 2. Experimental instruments

[0053] The experimental instruments mainly include electronic analytical balance, centrifuge, pipette, beaker, measuring cylinder, 1ml syringe, Chinese medicine pot, -80℃ refrigerator, ultrapure water machine, panoramic scanner, optical microscope, etc.

[0054] 2. Experimental Methods

[0055] 1. Establishment of mouse model of lipid metabolism disorder and group treatment

[0056] 48 C57BL / 6J mice were adaptively fed for one week and randomly divided into 6 groups according to body weight, with 8 mice in each group. Except for the blank group (Control) which was given normal maintenance feed, the mice in the other groups were given a high-fat diet (1.5wt% cholesterol, 0.5wt% bile salt, 10wt% lard, 10wt% egg yolk and 78wt% basic feed) for 2 weeks. Blood was collected from the eyelids and blood lipids were measured. Then, the mice were randomly divided into lipid metabolism disorder model group (Model), positive drug atorvastatin group (Positive), low-dose Huashi Jiangzhi Fang group (HSJZF-L), medium-dose Huashi Jiangzhi Fang group (HSJZF-Z), and high-dose Huashi Jiangzhi Fang group (HSJZF-H) according to the four blood lipid levels.

[0057] Each group was given medication according to the following regimen:

[0058] ① Blank group (Control): 0.9% saline was administered intragastrically at 10 mL / kg, once a day;

[0059] ② Lipid metabolism disorder model group (Model): 0.9% saline was administered orally at 10 mL / kg, once a day;

[0060] ③ Positive drug atorvastatin group (Positive): atorvastatin aqueous solution (concentration 0.5 mg / mL) was administered by intragastric administration at 5 mg / kg / day, once a day;

[0061] ④ Low-dose group of Huashijianzhifang (HSJZF-L): the concentrated solution obtained by concentrating the traditional Chinese medicine decoction of Example 1 (concentration of 0.425 g crude drug / mL) was administered by intragastric administration at 4.25 g / kg / day, once a day;

[0062] ⑤ The medium-dose group of Huashi Jianzhi Fang (HSJZF-Z): the concentrated solution obtained by concentrating the traditional Chinese medicine decoction of Example 1 (concentration of 0.85 g crude drug / mL) was administered by intragastric administration at 8.5 g / kg / day, once a day;

[0063] ⑥ High-dose group of Huashi Jianzhi Fang (HSJZF-H): The concentrated solution obtained by concentrating the traditional Chinese medicine decoction of Example 1 (concentration of 1.7 g crude drug / mL) was administered by intragastric administration at 17 g / kg / day, once a day.

[0064] All mice were gavaged for 10 weeks. During the experiment, the activity and mental state of the mice were observed daily, and their body weight was measured and recorded every two weeks.

[0065] 2. Serum lipid level determination

[0066] Whole blood was collected, placed at room temperature for 2 h, centrifuged at 3000 rpm for 15 min, and serum was collected. Biochemical kits were used to detect cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels.

[0067] 3. Liver function index detection

[0068] About 200 mg of liver tissue was placed in an EP tube, and the tissue was slurried on ice and centrifuged at 10,000 rpm for 30 min. The supernatant was taken and the liver superoxide dismutase (SOD), malondialdehyde (MDA), and total bile acid (TBA) were detected according to the instructions of the enzyme-linked immunosorbent (ELISA) kit.

[0069] 4. Liver morphology and pathological section staining

[0070] H&E staining: After the mouse liver was fixed in paraformaldehyde for 24 hours, the residual fixative was carefully cleaned with PBS solution (pH=7.5), and the tissue was trimmed and cut into regular shapes according to the observation requirements, dehydrated step by step with gradient alcohol, transparentized with xylene, embedded in paraffin, and sliced ​​on a slicer at 3um. After xylene, gradient alcohol and hydration, hematoxylin staining time was 5 minutes, eosin staining was 3 minutes, 1% hydrochloric acid alcohol was used for rapid differentiation, ammonia solution turned blue, and then washed with distilled water and dehydrated with regular gradient alcohol, transparentized with xylene, and sealed with neutral gum; the images were scanned with a panoramic scanner to observe the characteristics of tissue lesions.

[0071] Oil Red O staining: Take liver tissue of about 5mm×5mm×2mm in size and fix it in 4% paraformaldehyde solution for 24h. Dehydrate the fixed tissue samples step by step, then absorb the water on the surface of the tissue and add OCT embedding agent for embedding, sectioning (10μm), and freezing. After the frozen sections are rewarmed, they are immersed in distilled water twice, 3min each time, and then immersed in 60% isopropanol for 20-30sec, modified Oil Red O staining for 10-15min, 60% isopropanol differentiation twice, and washed with running water for 1min. Then use hematoxylin to counterstain for 1-2min, rinse with tap water for 10min to return to blue, wash slightly with distilled water, and finally use glycerol gelatin to seal the slides, and use an optical microscope for microscopic analysis and image collection.

[0072] 3. Experimental Results

[0073] 1. Effect of Huashi Jianzhi Recipe on Body Weight of Mice with Lipid Metabolism Disorder

[0074] The weight growth curves of mice in each group are shown in Figure 1 The final body weight of mice in each group was as shown in Figure 2 As shown in the figure, ***P<0.001.

[0075] In this experiment, a high-fat diet was used to induce a lipid metabolism disorder model in mice. There was no difference in the initial body weight of the six groups of mice. After 12 weeks of high-fat diet intervention, the body weight of the lipid metabolism disorder model group increased significantly compared with the blank group (P < 0.001). Figure 1-2 It can be seen that the order of the groups according to the amount of weight gain from small to large is: HSJZF-H<HSJZF-Z<Positive<HSJZF-L<Model, indicating that both the Huashi Jianzhi recipe and the positive drug can control the weight gain of mice fed a high-fat diet, among which the mice in the high-dose group of the Huashi Jianzhi recipe had the least weight gain and the difference was significant compared with the model group (P<0.001), indicating that the Huashi Jianzhi recipe has the effect of improving the body weight of mice with lipid metabolism disorders.

[0076] 2. Results of HE and Oil Red O staining of liver in each group

[0077] The results of liver HE staining in each group are as follows Figure 3 The results of Oil Red O staining of liver in each group are shown in Figure 4 shown.

[0078] Depend on Figure 3-4It can be seen that the liver cell structure of the blank group mice liver sections was normal, the cell nucleus was located in the center of the cell, and the cells were arranged radially around the central vein, and no fatty degeneration was observed; no red-stained lipids were observed in Oil Red O. Compared with the blank group, the liver cells in the model group were arranged in disorder, and lipid droplet vacuoles of different shapes, sizes, and numbers were observed in the cells, and a large number of red-stained lipids were observed in the Oil Red O sections. Compared with the model group, the degree of liver lesions in the positive drug group and the various dose groups of the Huashi Jianzhi prescription was alleviated, most of the liver cell structures were clearly visible, the number of lipid droplets was reduced, and the red-stained lipids of Oil Red O were significantly reduced.

[0079] 3. Determination of serum lipid levels in mice

[0080] The results of serum lipid levels in each group of mice are shown in Figure 5 As shown in the figures, *P<0.05, **P<0.01, ***P<0.001.

[0081] Depend on Figure 5 It can be seen that compared with the blank group, the levels of TC, TG, LDL-C, and HDL-C in the serum of the mice in the model group were significantly increased. Compared with the model group, the levels of TC, TG, and LDL-C in the serum of the mice in each dose group of the Huashi Jianzhi prescription were significantly reduced (P < 0.001), and the level of HDL-C in the serum was significantly increased (P < 0.05, P < 0.001). This shows that the Huashi Jianzhi prescription can improve the level of lipid metabolism disorders in mice induced by a high-fat diet.

[0082] 4. Determination of liver function indexes in mice

[0083] The results of liver function test of each group of mice are as follows Figure 6 As shown in the figure, ***P<0.001.

[0084] from Figure 6 It can be seen that compared with the blank group, the TBA content in the liver tissue of the mice in the model group was significantly increased. After the intervention of the Huashi Jiangzhi prescription, the TBA content in the liver tissue of the mice in each dose group of the Huashi Jiangzhi prescription decreased to varying degrees, and there was a statistical difference (P < 0.001). Compared with the blank group, the lipid peroxidation index SOD in the liver tissue of the mice in the model group was significantly reduced, while the peroxidation product MDA was significantly increased, indicating that there was a certain degree of oxidative damage in the liver of the mice in the model group. The lipid peroxidation index SOD in the liver tissue of the mice in each dose group of the Huashi Jiangzhi prescription was significantly increased (P < 0.001), and the peroxidation product MDA was significantly reduced (P < 0.001). It can be seen that the Huashi Jiangzhi prescription can significantly increase the activity of SOD and reduce the content of MDA, which indicates that the Huashi Jiangzhi prescription has a certain improvement effect on the liver function of mice with lipid metabolism disorders.

[0085] In summary, the Huashi Jianzhi recipe has the effect of improving the body weight of mice with lipid metabolism disorders; can reduce the degree of liver lesions in mice with lipid metabolism disorders, improve the structure of liver cells, and reduce the number of lipids and lipid droplets; can improve the level of lipid metabolism disorders in mice induced by a high-fat diet; and has a certain improvement effect on the liver function of mice with lipid metabolism disorders. This suggests that the Huashi Jianzhi recipe of the present invention has a good therapeutic effect on lipid metabolism disorders.

Claims

1. A prescription for eliminating dampness and reducing fat, characterized in that: The main active ingredients thereof include, by weight, 10-15 parts of bupleurum, 5-15 parts of curcuma, 10-15 parts of magnolia bark, 5-15 parts of cardamom, 10-30 parts of coix seeds, 3-10 parts of apricot kernel, 10-15 parts of atractylodes, and 5-15 parts of tangerine peel.

2. The dehumidifying and fat-reducing prescription according to claim 1, characterized in that: Its main active ingredients, measured by weight, include: 12 parts of bupleurum, 10 parts of curcuma, 12 parts of magnolia bark, 10 parts of cardamom, 20 parts of coix seed, 6 parts of apricot, 12 parts of atractylodes, and 10 parts of tangerine peel.

3. The prescription for eliminating dampness and reducing fat according to claim 1 or 2, characterized in that: Pharmaceutically acceptable excipients are also included.

4. The dehumidifying and fat-reducing prescription according to claim 1 or 2, characterized in that: The dosage form of the dampness-eliminating and fat-reducing prescription is decoction, oral solution, granules, powder, tablet, pill or capsule.

5. Use of the Huashi Jianzhi recipe according to any one of claims 1 to 4 in the preparation of a medicament for treating lipid metabolism disorders.

6. Use of the Huashi Jianzhi recipe according to any one of claims 1 to 4 in the preparation of a medicament for treating metabolic-related fatty liver disease.

7. Use of the Huashi Jianzhi recipe according to any one of claims 1 to 4 in the preparation of a medicament for treating dyslipidemia and / or obesity and / or fatty liver.

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