System for preventing and controlling crucian carp diseases by utilizing chlorella and probiotics and implementation method

Abstract

The invention relates to a system for preventing and controlling crucian carp diseases by utilizing chlorella and probiotics and an implementation method, and belongs to the technical field of aquaculture. Comprising a first culture barrel for culturing chlorella seed liquid, a second culture barrel for culturing probiotic liquid, a first pump body for conveying the chlorella seed liquid and the probiotic liquid in the first culture barrel and the second culture barrel to a pond, and a second pump body for conveying a water source to the first culture barrel and the second culture barrel, the spraying head is used for spraying chlorella liquid and probiotic liquid to the water surface of the pond; and the shading umbrella is used for shading culture of the first culture barrel and the second culture barrel. The system is low in cost and high in efficiency, time and labor can be saved, the phycomycetes can be used immediately after being cultured, and the phycomycetes putting and applying vitality is guaranteed to the maximum extent.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and in particular to a system and an implementation method for preventing and controlling crucian carp diseases by using chlorella and probiotics. Background Art

[0002] Crucian carp is an important freshwater farmed fish in my country, with huge farming scale and market demand. In the process of rapid development of crucian carp farming industry, crucian carp disease is an important factor threatening crucian carp production. As we all know, fish farming starts with water, good water breeds good fish, and the quality of water in the farming waters has a direct impact on the growth and development of crucian carp and disease prevention and control. Fish disease is mainly prevented, followed by treatment. Therefore, it is necessary to pay attention to the dynamic biological regulation of pond water during the farming process, enrich the level of beneficial bacteria and algae in the water, improve the self-purification ability of the water, and enhance the body's immunity through ecological farming, improve disease resistance, and reduce the occurrence of diseases.

[0003] Chlorella and probiotics are rapidly growing single-cell organisms. As renewable biological resources, they have the advantages of high nutritional value, safety, benefit, and sustainability. They have wide application value in aquaculture, animal husbandry, planting, and agricultural environmental protection. However, their cultivation and application still face many challenges, including high thresholds for algae strain screening and activation, efficient cultivation technology, and high culture conditions, preservation, and transportation costs. At present, Chlorella and photosynthetic bacteria probiotics are cultivated using common photobioreactors (such as CN1721523A- "Pipeline Photobioreactor for Microalgae Scale Culture"), and lactic acid bacteria are cultivated and produced using indoor fermentation tanks. Although pipeline photobioreactors and fermentation tanks have the advantages of low pollution and high yield, they have the problems of high construction cost, high energy consumption for cultivation and operation, and difficulty in cleaning the interior, and are not easily accepted and promoted by growers and breeders with a large demand for high-vitality high-quality algae and bacteria liquid.

[0004] In response to the above-mentioned phenomenon, the inventors have invented a system and method for preventing and controlling crucian carp diseases using Chlorella and probiotics, which utilizes natural water bodies to carry out low-cost, efficient cultivation and implementation of Chlorella and probiotics in ponds. Summary of the invention

[0005] The purpose of the present invention is to provide a system and implementation method for preventing and controlling crucian carp diseases using chlorella and probiotics, and a low-cost system and implementation method for efficiently cultivating chlorella and probiotics in ponds using natural water bodies, so as to provide high-quality and highly active algae strains and improve implementation efficiency.

[0006] The present invention provides a system for preventing and controlling carp diseases by using chlorella and probiotics, which adopts the following technical scheme:

[0007] A system for preventing and controlling diseases of crucian carp using chlorella and probiotics, comprising:

[0008] The first culture barrel is used to culture the Chlorella seed solution;

[0009] The second culture barrel is used to culture the probiotic liquid;

[0010] A first pump body, used for infusing liquid into the pond, wherein an input end of the first pump body is connected to the bottom ends of the first culture barrel and the second culture barrel;

[0011] A second pump body, used for conveying water to the first culture barrel and the second culture barrel;

[0012] The injection head is arranged on the water surface of the pond, and the injection head and the output end of the first pump body are connected with an infusion tube group;

[0013] A shading umbrella is used to shade the first culture barrel and the second culture barrel.

[0014] Preferably, it also includes,

[0015] A base, fixedly sleeved on the circumference of the first culture barrel and the second culture barrel;

[0016] The switch box is electrically connected to the first pump body and the second pump body.

[0017] Preferably, the barrel bodies of the first culture barrel and the second culture barrel are either white, transparent or translucent.

[0018] Preferably, the first culture barrel and the second culture barrel are respectively connected to the input end of the first pump body by a first tube group;

[0019] The first pipe group includes a first main pipe and a first branch pipe, the first main pipe is connected to the input end of the first pump body, two groups of the first branch pipes are arranged, the two groups of the first branch pipes are commonly connected to one end of the first main pipe away from the first pump body, the two groups of the first branch pipes are respectively connected to the bottom ends of the first culture barrel and the second culture barrel, and the first valves are respectively arranged on the walls of the two groups of the first branch pipes.

[0020] Preferably, the infusion tube set includes an infusion main tube and an infusion branch tube;

[0021] The infusion main pipe is connected to the output end of the second pump body, and the infusion branch pipes are provided with multiple groups, and the multiple groups of infusion branch pipes are commonly connected to one end of the infusion main pipe away from the first pump body, and the tube walls of the multiple groups of infusion branch pipes are respectively provided with second valves;

[0022] The injection heads include a plurality of groups having the same number as the infusion branches.

[0023] Preferably, the top ends of the first culture barrel and the second culture barrel are respectively connected to the output end of the second pump body with a second tube group;

[0024] The second pipe group includes a second main pipe and a second branch pipe, the second main pipe is connected to the output end of the second pump body, two groups of the second branch pipes are provided, the two groups of the second branch pipes are commonly connected to one end of the second main pipe away from the second pump body, the two groups of the second branch pipes are respectively connected to the top of the first culture barrel and the second culture barrel, and the two groups of the second branch pipes are respectively provided with a third valve;

[0025] The input end of the second pump body is connected to a water source pipe, and a first filter screen is arranged between the input end of the second pump body and the water source pipe, and the first filter screen is 80 meshes. The two groups of the second branch pipes are respectively provided with a second filter screen at one end close to the first culture barrel and the second culture barrel, and the second filter screen is 300 meshes.

[0026] Preferably, the sunshade umbrella comprises an umbrella cloth and an umbrella handle;

[0027] The umbrella handle includes a tube body and a sliding rod, the sliding rod is connected to the umbrella cloth, the sliding rod is slidably connected to the tube body along the vertical direction, the sliding rod wall is provided with multiple groups of first through holes evenly arranged along the vertical direction, the tube body wall is provided with a second through hole, and the second through hole and the first through hole are jointly plugged with a fixing pin.

[0028] The present invention also provides a method for implementing the system for preventing and controlling carp diseases using chlorella and probiotics, comprising the following steps:

[0029] S1. Pretreatment of the first culture barrel and the second culture barrel: cleaning the first culture barrel and the second culture barrel, and exposing the first culture barrel and the second culture barrel to sunlight for 1-2 days with the lids opened;

[0030] S2. Cultivation of Chlorella seed liquid and probiotic liquid: add 15-30% of the total culture volume of the first culture barrel (1) of Chlorella seed liquid to the first culture barrel (1), then add 0.5-0.8% of the total culture volume of the first culture barrel (1) of nutrient mother liquid, open the first valve (313) and the first pump body (3) to inject water into the first culture barrel (1) until the first culture barrel (1) is full, and cover the barrel cover of the first culture barrel (1); add 15-30% of the total culture volume of the first culture barrel (1) of Chlorella seed liquid to the second culture barrel (1), and then add 0.5-0.8% of the total culture volume of the first culture barrel (1) of nutrient mother liquid to the second culture barrel (1). The probiotics stock solution accounting for 10-20% of the total culture volume of the culture barrel (2) is added, the probiotics stock solution accounting for 10-20% of the total culture volume of the second culture barrel (2) is added, molasses accounting for 5% of the total culture volume of the second culture barrel (2) is added, and a growth regulator accounting for 0.2-0.25% of the total culture volume of the second culture barrel (2) is added, the first valve (313) and the first pump body (3) are opened to inject water into the second culture barrel (2) until the second culture barrel (2) is filled, and the second culture barrel is covered;

[0031] S3, adjusting the sunshade umbrella so that the Chlorella seed solution in the first culture bucket and the probiotic solution in the second culture bucket in step S2 grow in an environment with a temperature of 20-35° C. and a light intensity of 3000-15000 lux, and the growth cycle is 3-5 days;

[0032] S4. Detect the growth status of the chlorella seed solution and the probiotic solution obtained in step S3 by visual observation with the naked eye or microscopic observation with a microscope. The chlorella solution changes from light green to dark green, has a grassy smell, and more than 90% of the chlorella cells are evenly arranged in the microscopic field of view; the pH of the probiotic solution is less than 4, and the color of the photosynthetic bacteria is dark red;

[0033] S5, opening the first valve and the second valve, and starting the first pump, so that the Chlorella seed liquid in the first culture barrel and the probiotic liquid in the second culture barrel flow into the pond;

[0034] S6. After the Chlorella seed solution in the first culture barrel and the probiotic solution in the second culture barrel are released, the first culture barrel and the second culture barrel are cleaned and exposed to sunlight for 1-2 days before entering the next round of cultivation to ensure that the algae and fungi biological regulation in the pond is carried out four times a month.

[0035] Preferably, each liter of nutrient solution in step S2 comprises the following components in parts by weight: 200-300 parts of organic amino acid fertilizer, 20-30 parts of vitamin C, 15-25 parts of magnesium sulfate, 8-12 parts of dipotassium hydrogen phosphate, 6.5-12.5 parts of calcium chloride, 3-8 parts of sodium carbonate, 1-2 parts of ammonium ferric citrate, 0.05-0.06 parts of zinc sulfate, and 0.42-0.48 parts of manganese chloride.

[0036] Preferably, the probiotics in step S2 include any one or more of lactic acid bacteria, bacillus and photosynthetic bacteria;

[0037] The lactic acid bacteria include the following components in parts by weight: 35-40 parts of Lactobacillus parafarraginis, 15-20 parts of Lactobacillus shenzhenensis, 15-20 parts of Lactobacillus buchneri, 3-6 parts of Lactobacillus rapi, 3-6 parts of Lactobacillus plantarum, 3-6 parts of Lactobacillus harbinensis, 3-6 parts of Lactobacillus rhamnosus, 3-6 parts of Lactobacillus casei, and 3-6 parts of Lactobacillus brevis.

[0038] In summary, the present invention includes the following beneficial technical effects:

[0039] 1. The present invention provides a first culture barrel and a second culture barrel to culture chlorella seed liquid and probiotic liquid respectively, and transports the cultivated chlorella seed liquid and probiotic liquid to a pond through a first pump body, so that the chlorella seed liquid and probiotic liquid are sprayed 360 degrees to the water surface of the pond through a spray head, so that the chlorella seed liquid and probiotic liquid are more evenly distributed in the pond; the second pump body is connected to a water source, and in the process of culturing the chlorella seed liquid and probiotic liquid, the second pump body transports water to the first culture barrel and the second culture barrel, and the sunshade is used to shade the first culture barrel and the second culture barrel, so that the first culture barrel and the second culture barrel are at a suitable light intensity.

[0040] 2. The implementation method of the system for preventing and controlling crucian carp diseases using chlorella and probiotics in the present application includes pretreatment of the first culture barrel and the second culture barrel, cultivation of chlorella seed solution and probiotic solution, observation of the growth status of chlorella seed solution and probiotic solution, transportation of chlorella seed solution and probiotic solution into the pond, and cultivation of the next round of chlorella seed solution and probiotic solution. The chlorella solution and probiotic solution cultured by the method are ready for use and can be used regularly and effectively four times a month. The bacteria and algae work together to purify water quality, enhance the body's immunity, greatly improve the disease resistance of crucian carp, and effectively reduce the occurrence of diseases in crucian carp.

[0041] 3. The nutrient solution and growth regulator in the present application can meet the needs of rapid growth of Chlorella and probiotics respectively, and the light intensity of the culture can be regulated by raising and lowering the sunshade umbrella to ensure a 100% culture success rate and more than 90% effective culture purity. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 is a schematic diagram of the structure of a system for preventing and controlling crucian carp diseases using chlorella and probiotics provided by an embodiment of the present invention;

[0043] Figure 2 A system for preventing and controlling crucian carp diseases using chlorella and probiotics prepared according to the embodiments of the present application;

[0044] Figure 3 is a site layout diagram of the injection head in the embodiment of the present application;

[0045] Figure 4 It is a schematic diagram for showing the structure of the first tube group;

[0046] Figure 5 This is a schematic diagram showing the structure of the second tube group.

[0047] Explanation of the reference numerals: 1. first culture barrel; 2. second culture barrel; 3. first pump body; 31. first tube group; 311. first main pipe; 312. first branch pipe; 313. first valve; 32. infusion tube group; 321. infusion main pipe; 322. infusion branch pipe; 323. second valve; 4. injection head; 5. second pump body; 51. water source pipe; 511. first filter screen; 52. second tube group; 521. second main pipe; 522. second branch pipe; 523. third valve; 524. second filter screen; 6. base; 7. switch box; 8. sunshade umbrella; 81. umbrella cloth; 82. umbrella handle; 821. tube body; 822. slide rod; 823. first through hole; 824. second through hole; 825. fixing pin. DETAILED DESCRIPTION

[0048] The following is combined with Figure 1-5 The present invention is described in further detail.

[0049] Example

[0050] Example 1

[0051] The present invention provides a system for preventing and controlling diseases of crucian carp using chlorella and probiotics. Figure 1 and Figure 2 , including a first culture barrel 1 and a second culture barrel 2, the first culture barrel 1 and the second culture barrel 2 are respectively used to culture chlorella seed liquid and probiotic liquid; the bottom ends of the first culture barrel 1 and the second culture barrel 2 are connected with a first pump body 3, and the first pump body 3 is used to transport the chlorella seed liquid and the probiotic liquid in the first culture barrel 1 and the second culture barrel 2 to a pond where crucian carp is cultured, referring to Figure 3 A spray head 4 is fixedly arranged at the center of the pond surface. The spray head 4 in this embodiment adopts a suspended shower nozzle. The spray head 4 is connected to the output end of the first pump body 3 to spray the chlorella seed liquid and the probiotic liquid 360 degrees onto the pond water surface.

[0052] Reference Figure 1 and Figure 4 The first culture barrel 1 and the second culture barrel 2 are respectively connected to the input end of the first pump body 3 with a first pipe group 31, the first pipe group 31 includes a first main pipe 311 and a first branch pipe 312, the first main pipe 311 is connected to the input end of the first pump body 3, and two groups of first branch pipes 312 are provided, the two groups of first branch pipes 312 are respectively connected to the bottom ends of the first culture barrel 1 and the second culture barrel 2, the two groups of first branch pipes 312 are connected to the end of the first main pipe 311 away from the first pump body 3 through a three-way pipe, and the pipe walls of the two groups of first branch pipes 312 are respectively installed with first valves 313. That is, when the chlorella seed liquid and probiotic liquid are transported to the pond in advance, the first pump body 3 and the valve are opened, and the first pump body 3 transports the chlorella seed liquid and probiotic liquid in the first culture barrel 1 and the second culture barrel 2 to the pond.

[0053] Reference Figure 1 and Figure 4 The output end of the first pump body 3 is connected to the injection head 4 with an infusion tube group 32, the infusion tube group 32 includes an infusion main pipe 321 and an infusion branch pipe 322, the infusion main pipe 321 is connected to the output end of the second pump body 5, and multiple groups of infusion branch pipes 322 are provided, and the multiple groups of infusion branch pipes 322 are commonly connected to the end of the infusion main pipe 321 away from the first pump body 3, and the tube walls of the multiple groups of infusion branch pipes 322 are respectively provided with second valves 323, and the injection head 4 includes multiple groups of the same number as the infusion branch pipes 322. There are two groups of ponds in this embodiment, and each group of ponds is placed with an injection head 4, that is, there are two groups of infusion branch pipes 322 in this embodiment. When the first pump body 3 and the first valve 313 are opened, the second valve 323 is opened to transport the chlorella seed liquid and the probiotic liquid to the two groups of ponds.

[0054] Reference Figure 1 and Figure 5 The top of the first culture barrel 1 and the top of the second culture barrel 2 are connected to a second pump body 5, and the input end of the second pump body 5 is connected to a water source pipe 51 to transport water to the first culture barrel 1 and the second culture barrel 2. Figure 3 The water source in this embodiment can be selected as any one of natural water source or tap water, and a first filter screen 511 is installed between the input end of the second pump body 5 and the water source pipe 51, and the first filter screen 511 is 80 meshes to filter the water source.

[0055] Reference Figure 1 and Figure 5 The tops of the first culture barrel 1 and the second culture barrel 2 are respectively connected to the output end of the second pump body 5 with a second pipe group 52, the second pipe group 52 includes a second main pipe 521 and a second branch pipe 522, the second main pipe 521 is connected to the output end of the second pump body 5, and the second branch pipe 522 is provided with two groups, the two groups of second branch pipes 522 are respectively connected to the tops of the first culture barrel 1 and the second culture barrel 2, wherein the second branch pipes 522 are respectively installed with a second filter screen 524 at one end close to the first culture barrel 1 and the second culture barrel 2, and the second filter screen is 300 meshes to further filter the water source, the two groups of second branch pipes 522 are connected to the end of the second main pipe 521 away from the second pump body 5 through a three-way pipe, and the walls of the two groups of second branch pipes 522 are respectively installed with a third valve 523. In the process of culturing the chlorella seed liquid and the probiotic liquid, the second pump body 5 and the third valve 523 are opened to transport the water source into the first culture barrel 1 and the second culture barrel 2.

[0056] Reference Figure 1 and Figure 4The first culture barrel 1 and the second culture barrel 2 are fixedly sleeved with a base 6, which plays a role in fixing the first culture barrel 1 and the second culture barrel 2; the base 6 is installed with a switch box 7 electrically connected to the first pump body 3 and the second pump body 5 to control the start and stop of the first pump body 3 and the second pump body 5. The base 6 cover is fixedly installed with a vertical light-shielding umbrella 8, which is used to shield the first culture barrel 1 and the second culture barrel 2 in the state of culturing the chlorella seed liquid and the probiotic liquid, and the barrel bodies of the first culture barrel 1 and the second culture barrel 2 in the embodiment are either white, transparent or translucent. The sunshade umbrella 8 includes an umbrella cloth 81 and an umbrella handle 82; the umbrella handle 82 includes a tube body 821 and a slide bar 822, the slide bar 822 is connected to the umbrella cloth 81, and the slide bar 822 is slidably connected to the tube body 821 along the vertical direction. The wall of the slide bar 822 is provided with a plurality of first through holes 823 evenly arranged along the vertical direction, and the wall of the tube body 821 is provided with a second through hole 824, and the second through hole 824 and the first through hole 823 are jointly plugged with a fixing pin 825. The height of the sunshade umbrella 8 is adjusted by sliding the slide bar 822, and the fixing pin 825 is inserted when the second through hole 824 corresponds to one of the first through holes 823 to fix the height of the sunshade umbrella 8, so that the first culture bucket 1 and the second culture bucket 2 are in a suitable light intensity state.

[0057] Example 2

[0058] The present invention also provides a method for implementing a system for preventing and controlling carp diseases using chlorella and probiotics, comprising the following steps:

[0059] S1. Pretreatment of the first culture barrel and the second culture barrel: cleaning the first culture barrel and the second culture barrel, and exposing the first culture barrel and the second culture barrel to sunlight for 1-2 days with the lids opened;

[0060] S2. Cultivation of Chlorella seed solution and probiotic solution: In this embodiment, a first culture barrel with a capacity of 160 L and a second culture barrel are selected; 24 L of Chlorella seed solution is added to the first culture barrel, and then 800 mL of nutrient mother solution is added, the first valve and the first pump body are opened to inject water into the first culture barrel until the first culture barrel is full, and the barrel cover of the first culture barrel is covered; 16 L of probiotic seed solution is added to the second culture barrel, and then 8 L of molasses and 320 mL of growth regulator are added, the second valve and the first pump body are opened to inject water into the second culture barrel until the second culture barrel is full, and the second culture barrel is covered;

[0061] S3, adjusting the sunshade umbrella so that the Chlorella seed solution in the first culture bucket and the probiotic solution in the second culture bucket in step S2 grow in an environment with a temperature of 20-35° C. and a light intensity of 3000-15000 lux, and the growth cycle is 3-5 days;

[0062] S4. Detect the growth status of the chlorella seed solution and the probiotic solution obtained in step S3 by visual observation with the naked eye or microscopic observation with a microscope. The chlorella solution changes from light green to dark green, has a grassy smell, and more than 90% of the chlorella cells are evenly arranged in the microscopic field of view; the pH of the probiotic solution is less than 4, and the color of the photosynthetic bacteria is dark red;

[0063] S5, opening the first valve and the second valve, and starting the first pump, so that the Chlorella seed liquid in the first culture barrel and the probiotic liquid in the second culture barrel flow into the pond;

[0064] S6. After the Chlorella seed solution in the first culture barrel and the probiotic solution in the second culture barrel are released, the first culture barrel and the second culture barrel are cleaned and exposed to sunlight for 1-2 days before entering the next round of cultivation to ensure that the algae and fungi in the pond are biologically regulated 4 times a month.

[0065] Each liter of nutrient solution in step S2 includes the following components in parts by weight: 200-300 parts of organic amino acid fertilizer, 20-30 parts of vitamin C, 15-25 parts of magnesium sulfate, 8-12 parts of dipotassium hydrogen phosphate, 6.5-12.5 parts of calcium chloride, 3-8 parts of sodium carbonate, 1-2 parts of ammonium ferric citrate, 0.05-0.06 parts of zinc sulfate, and 0.42-0.48 parts of manganese chloride.

[0066] The probiotics in step S2 include any one or more of lactic acid bacteria, bacillus and photosynthetic bacteria;

[0067] The lactic acid bacteria include the following components in parts by weight: 35-40 parts of Lactobacillus parafarraginis, 15-20 parts of Lactobacillus shenzhenensis, 15-20 parts of Lactobacillus buchneri, 3-6 parts of Lactobacillus rapi, 3-6 parts of Lactobacillus plantarum, 3-6 parts of Lactobacillus harbinensis, 3-6 parts of Lactobacillus rhamnosus, 3-6 parts of Lactobacillus casei, and 3-6 parts of Lactobacillus brevis.

[0068] Test example

[0069] Test Example 1

[0070] Case 1:

[0071] In May 2023, the Yangzhong Base of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences selected two ponds with an area of ​​2.5 acres each to carry out a comparative test on ecological breeding for crucian carp disease prevention and control. Among them, Pond 1# was used as the experimental group to carry out the coordinated ecological breeding of crucian carp and algae, and Pond 2# was used as the control group to carry out traditional breeding of crucian carp. The two breeding modes have a stocking specification of 1250 tails / kg and a stocking density of 2500 tails / acre. All the fry are self-bred from the base, with a stocking specification of 1.5kg / tail of silver carp, and a stocking density of 8 tails / acre to regulate the water quality. Pond 1# is adjacent to Pond 2#. A system using Chlorella and probiotics to prevent and control crucian carp diseases is placed between Pond 1# and Pond 2#. Water and electricity were connected in early May, and bacteria and algae were cultivated according to the steps in Example 2.

[0072] The experimental group 1# pond used bacteria and Chlorella 4 times a month, while the control group 2# pond did not use them at all. The pond water quality was tested regularly during the breeding period, and other feeding and other operations remained the same. During the breeding period, the crucian carp in the experimental group and the control group did not get sick. Analysis of the water quality test results showed that the total phosphorus (0.31±0.02) mg / L and total nitrogen (1.05±0.35) mg / L in the experimental group were lower than the total phosphorus (0.35±0.03) mg / L and total nitrogen (1.31±0.21) mg / L in the control group. The water quality test results showed that the experimental group carried out the ecological breeding of crucian carp and algae and regularly used bacteria to regulate the water quality, which could keep the eutrophication indicators such as total phosphorus and total nitrogen in the water at a relatively low state, maintain the freshness of the water quality, and be conducive to the healthy growth of crucian carp.

[0073] Starting from February 2024, the average size of crucian carp in the experimental group's crucian carp, fungi and algae collaborative ecological farming model was 0.51kg / tail, and the average yield per mu was 2972kg. Calculated at 18 yuan / kg, the average output value per mu can reach 21,396 yuan. Excluding the average cost per mu of 13,324 yuan, the profit per mu can reach 8,072 yuan, which is very beneficial. The average size of crucian carp in the control group's traditional farming model was 0.45kg / tail, and the average yield per mu was 2792kg. Calculated at 18 yuan / kg, the average output value per mu can reach 18,633 yuan. Excluding the average cost per mu of 13,210 yuan, the profit per mu can reach 5,423 yuan. In comparison, the crucian carp, fungi and algae collaborative ecological farming model has a larger average size, a higher yield per mu, and the benefits are 48.84% higher than those of traditional crucian carp farming models.

[0074] In order to further verify the disease resistance of crucian carp cultured in ponds 1# and 2#, a further indoor Aeromonas hydrophila culture comparison test was conducted using the captured crucian carp in February 2024. 40 crucian carp from each culture mode were randomly captured and challenged with Aeromonas hydrophila by intraperitoneal injection at a concentration of 1.92x10 7CFU / mL (the attack concentration is the half-lethal concentration determined by the preliminary experiment), and another 40 crucian carps were injected with PBS buffer as a blank control. As shown in Table 1, the changes in the number of surviving crucian carp in each group were continuously observed within 72 hours. The results showed that deaths occurred 24 hours after bacterial infection, and the growth trend of the control group was faster than that of the experimental group. The mortality rate was significantly higher than that of the experimental group (P<0.05), and there was no death in the blank group. The experimental results show that the crucian carp in the experimental group under the crucian carp, bacteria and algae collaborative ecological farming model has higher antiviral ability, and the survival rate of the virus attack is 240% higher than that of the traditional model.

[0075]

[0076] Table 1 Changes in the number of crucian carp surviving in the two groups within 72 hours of Aeromonas hydrophila infection

[0077] Test Example 2

[0078] In April 2024, the Yangzhong Base of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences further selected two ponds with an area of ​​2.5 mu each to carry out comparative experiments on ecological breeding for crucian carp disease prevention and control. Among them, Pond 3# was used as the experimental group to carry out coordinated ecological breeding of crucian carp, with a stocking density of 2,000 per mu and an average size of 87.37g; in addition, Pond 4# was used as the control group to carry out traditional breeding of crucian carp, with a stocking density of 2,000 per mu and an average size of 85.41g. Pond 3# and Pond 4# are adjacent to each other. A system for preventing and controlling crucian carp diseases using chlorella and probiotics is placed between Pond 3# and Pond 4#, and water and electricity are connected to cultivate bacteria and algae according to the steps in Example 2.

[0079] The experimental group 3# pond used bacteria and Chlorella 4 times a month, while the control group 4# pond did not use them at all. The pond water quality was tested regularly during the culture period, and other feeding and other operations remained the same. As shown in Table 2 below, the statistical analysis of the water quality test results shows that at 90 days of culture, the total nitrogen content of the experimental group and the control group was significantly higher than the initial total nitrogen content (P<0.05), and the total nitrogen content of the control group was significantly higher than that of the experimental group at 60 days. There was no significant difference in total nitrogen content in the rest of the period. The ammonia nitrogen content was generally high. The ammonia nitrogen content of the two groups at 90 days was significantly higher than that at 0 days, and the ammonia nitrogen content of the experimental group was significantly lower than that of the control group at 30 days and 60 days (P<0.05). The nitrate nitrogen and nitrous acid contents of the experimental group were significantly lower than those of the control group at 30 days, 60 days, and 90 days, and the nitrate nitrogen content of the experimental group at 90 days was the same as that at 0 days, and the nitrate nitrogen content of the control group was significantly increased (P<0.05). At 0d, 60d, and 90d, the total phosphorus content in the experimental group was significantly lower than that in the control group (P<0.05), and there was no significant difference in the total phosphorus content in the experimental group in the four samplings, which was relatively stable, while the total phosphorus content in the control group gradually increased. The above results show that the bacteria-algae system can effectively control the increase of total phosphorus content in the process of crucian carp farming, keep the ammonia nitrogen, nitrate nitrogen, and nitrous acid content at a generally low level, maintain the water quality of the experimental group crucian carp bacteria-algae collaborative ecological farming at a better state, significantly improve the living environment of crucian carp, and reduce the probability of disease occurrence.

[0080]

[0081] Note: Different lowercase letters represent significant differences within the same group at different periods (P<0.05), and different uppercase letters represent significant differences between different groups at the same period (P<0.05).

[0082] Table 2 Water quality indicators under the two aquaculture modes at different periods

[0083] The above are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A system for preventing and controlling crucian carp diseases using chlorella and probiotics, characterized in that: include, A first culture barrel (1) is used for culturing a Chlorella seed solution; The second culture barrel (2) is used for culturing the probiotic liquid; A first pump body (3) is used for pumping liquid into the pond, wherein the input end of the first pump body (3) is connected to the bottom ends of the first culture barrel (1) and the second culture barrel (2); A second pump body (5) is used to transport water to the first culture barrel (1) and the second culture barrel (2); The spray head (4) is arranged on the water surface of the pond, and the spray head (4) and the output end of the first pump body (3) are connected to a liquid infusion tube set (32); A light-shielding umbrella (8) is used to shield the first culture barrel (1) and the second culture barrel (2) from light.

2. A system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 1, characterized in that: Also includes, A base (6) is fixedly mounted on the circumference of the first culture barrel (1) and the second culture barrel (2); The switch box (7) is electrically connected to the first pump body (3) and the second pump body (5).

3. A system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 1, characterized in that: The barrel bodies of the first culture barrel (1) and the second culture barrel (2) are either white, transparent or translucent.

4. The system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 1, characterized in that: The first culture barrel (1) and the second culture barrel (2) are respectively connected to the input end of the first pump body (3) via a first tube group (31); The first tube group (31) comprises a first main tube (311) and a first branch tube (312). The first main tube (311) is connected to the input end of the first pump body (3). Two groups of the first branch tubes (312) are provided. The two groups of the first branch tubes (312) are commonly connected to an end of the first main tube (311) away from the first pump body (3). The two groups of the first branch tubes (312) are respectively connected to the bottom ends of the first culture barrel (1) and the second culture barrel (2). The tube walls of the two groups of the first branch tubes (312) are respectively provided with first valves (313).

5. A system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 4, characterized in that: The infusion tube set (32) comprises an infusion main tube (321) and an infusion branch tube (322); The infusion main pipe (321) is connected to the output end of the second pump body (5), and the infusion branch pipes (322) are provided in multiple groups. The multiple groups of infusion branch pipes (322) are commonly connected to one end of the infusion main pipe (321) away from the first pump body (3), and the walls of the multiple groups of infusion branch pipes (322) are respectively provided with second valves (323); The injection head (4) includes a plurality of groups having the same number as the infusion branch tubes (322).

6. The system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 1, characterized in that: The top ends of the first culture barrel (1) and the second culture barrel (2) are respectively connected to the output end of the second pump body (5) by a second tube group (52); The second pipe group (52) comprises a second main pipe (521) and a second branch pipe (522). The second main pipe (521) is connected to the output end of the second pump body (5). Two groups of the second branch pipes (522) are provided. The two groups of the second branch pipes (522) are commonly connected to an end of the second main pipe (521) away from the second pump body (5). The two groups of the second branch pipes (522) are respectively connected to the top of the first culture barrel (1) and the top of the second culture barrel (2). The two groups of the second branch pipes (522) are respectively provided with a third valve (523). The input end of the second pump body (5) is connected to a water source pipe (51), and a first filter screen (511) is arranged between the input end of the second pump body (5) and the water source pipe (51), and the first filter screen (511) has an 80-mesh size. The two groups of second branch pipes (522) are respectively provided with a second filter screen (524) at one end close to the first culture barrel (1) and the second culture barrel (2), and the second filter screen has a 300-mesh size.

7. The system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 1, characterized in that: The sunshade umbrella (8) comprises an umbrella cloth (81) and an umbrella handle (82); The umbrella handle (82) comprises a tube body (821) and a sliding rod (822), wherein the sliding rod (822) is connected to the umbrella cloth (81), and the sliding rod (822) is slidably connected to the tube body (821) in the vertical direction, and the wall of the sliding rod (822) is provided with a plurality of first through holes (823) evenly arranged in the vertical direction, and the wall of the tube body (821) is provided with a second through hole (824), and a fixing pin (825) is inserted into the second through hole (824) and the first through hole (823).

8. A method for implementing the system for preventing and controlling crucian carp diseases using chlorella and probiotics according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1, pretreatment of the first culture barrel (1) and the second culture barrel (2): cleaning the first culture barrel (1) and the second culture barrel (2), and exposing the first culture barrel (1) and the second culture barrel (2) to sunlight for 1-2 days with the lids opened; S2. Cultivation of Chlorella seed liquid and probiotic liquid: add 15-30% of the total culture volume of the first culture barrel (1) of Chlorella seed liquid to the first culture barrel (1), then add 0.5-0.8% of the total culture volume of the first culture barrel (1) of nutrient mother liquid, open the first valve (313) and the first pump body (3) to inject water into the first culture barrel (1) until the first culture barrel (1) is full, and cover the barrel cover of the first culture barrel (1); add 15-30% of the total culture volume of the first culture barrel (1) of Chlorella seed liquid to the second culture barrel (1), and then add 0.5-0.8% of the total culture volume of the first culture barrel (1) of nutrient mother liquid to the second culture barrel (1). The probiotics stock solution accounting for 10-20% of the total culture volume of the culture barrel (2) is added, the probiotics stock solution accounting for 10-20% of the total culture volume of the second culture barrel (2) is added, molasses accounting for 5% of the total culture volume of the second culture barrel (2) is added, and a growth regulator accounting for 0.2-0.25% of the total culture volume of the second culture barrel (2) is added, the first valve (313) and the first pump body (3) are opened to inject water into the second culture barrel (2) until the second culture barrel (2) is filled, and the second culture barrel is covered; S3, adjusting the light-shielding umbrella (8) so that the Chlorella seed solution in the first culture barrel (1) and the probiotic solution in the second culture barrel (2) in step S2 grow in an environment with a temperature of 20-35° C. and a light intensity of 3000-15000 lux, and the growth period is 3-5 days; S4. Detect the growth status of the chlorella seed solution and the probiotic solution obtained in step S3 by visual observation with the naked eye or microscopic observation with a microscope. The chlorella solution changes from light green to dark green, has a grassy smell, and more than 90% of the chlorella cells are evenly arranged in the microscopic field of view; the pH of the probiotic solution is less than 4, and the color of the photosynthetic bacteria is dark red; S5, opening the first valve (313) and the second valve (323), and starting the first pump (3), so that the Chlorella seed liquid in the first culture barrel (1) and the probiotic liquid in the second culture barrel (2) flow into the pond; S6. After the Chlorella seed solution in the first culture barrel (1) and the probiotic solution in the second culture barrel (2) are released, the first culture barrel (1) and the second culture barrel (2) are cleaned and exposed to sunlight for 1-2 days before entering the next round of cultivation, ensuring that the algae and fungi in the pond are biologically regulated four times a month.

9. The method for implementing a system for preventing and controlling crucian carp diseases using chlorella and probiotics according to claim 8, characterized in that: Each liter of the nutrient solution in step S2 includes the following components in parts by weight: 200-300 parts of organic amino acid fertilizer, 20-30 parts of vitamin C, 15-25 parts of magnesium sulfate, 8-12 parts of dipotassium hydrogen phosphate, 6.5-12.5 parts of calcium chloride, 3-8 parts of sodium carbonate, 1-2 parts of ammonium ferric citrate, 0.05-0.06 parts of zinc sulfate, and 0.42-0.48 parts of manganese chloride.

10. The method for implementing a system for preventing and controlling carp diseases using chlorella and probiotics according to claim 8, characterized in that: The probiotic bacteria in step S2 include any one or more of lactic acid bacteria, bacillus and photosynthetic bacteria; The lactic acid bacteria include the following components in parts by weight: 35-40 parts of s__Lactobacillus_parafarraginis, 15-20 parts of s__Lactobacillus_shenzhenensis, 15-20 parts of s__Lactobacillus_buchneri, 3-6 parts of s__Lactobacillus_rapi, 3-6 parts of s__Lactobacillus_plantarum, s__Lactobacillus_harbinensis 3-6 parts, s__Lactobacillus_rhamnosus 3-6 parts, s__Lactobacillus_casei 3-6 parts, s__Lactobacillus_brevis 3-6 parts.

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