Globule nocardia strain and use of it for removing organic sulfur in fossil fuel
A technology of Nocardia parvum and fossil fuels, applied in bacteria, petroleum industry, refined hydrocarbon oil, etc., can solve problems such as specific sulfur removal without systematic research, achieve broad industrial application potential, organic resistance The effect of strong solvent ability and strong desulfurization ability
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Embodiment 1
[0069] Screen Nocardia globerula R-9 bacterial strain, its step is as follows:
[0070] Get the aerobic activated sludge sample from the sewage treatment tank of the oil refinery of Dagang Petrochemical Company in China, and take 5 grams of the sample and suspend it in the sulfur-free basic medium (the composition of the medium: 1000ml water, KH 2 PO 4 , 2.44g; Na 2 HPO 4 12H 2 O 14.03g; NH 4 Cl, 2.00g; MgCl 2 ·6H 2 O, 0.36g; CaCl 2 2H 2 O, 1; FeCl 3 1 mg; MnCl 4H 2 O, 4 mg; CuCl 2 , 0.755mg; glycerol 0.8ml or glucose 1-3g, 20 minutes 121 ℃ autoclave), put in a shaker and mix for 30 minutes, let it stand, absorb the upper suspension and transfer it to the nutrient medium (composition g / g: NaCl 1.0%; yeast extract powder 0.5%, tryptone 1%, autoclaved for 20 minutes), put in a shaker and cultivate for 24 hours at 150 rpm. Draw 5ml of the bacterial solution and add it to 100ml of sterilized basal medium, and then add dibenzothiophene DBT-ethanol solution to make the ...
Embodiment 2
[0073] To prepare a liquid culture of cells from the Nocardia globerula R-9 strain:
[0074] Pick a small ball Nocardia globerula R-9 strain cultured on the slope of nutrient agar and inoculate it with a platinum loop in a sterilized test tube, add sterilized DBT-ethanol solution to make the DBT concentration 0.1mmol, and inoculate it at 30 Cultivate in a biological incubator at 150 rpm for 16-24 hours. Take 1ml of the bacterial solution obtained from the culture and inoculate it into a 300ml Erlenmeyer flask equipped with 100ml of basic inorganic salt medium (pH=7.0), and then add sterilized DBT-ethanol solution to make the DBT concentration 0.1-0.5mmol. The Erlenmeyer flask was placed in a biological incubator at 30° C. and cultured at 150 rpm for 48 hours to obtain a liquid culture solution of Nocardia globerula strain R-9 cells.
Embodiment 3
[0076] Preparation of non-growing resting cell fluid of Nocardia globerula strain R-9:
[0077] The liquid culture solution of bacterial strain R-9 cells obtained by the method of Example 2 was centrifuged (8000 rpm) for 10 minutes, and the supernatant suspension was poured off. The centrifuged precipitate was washed with an appropriate amount of normal saline for 3 times, the obtained centrifugal precipitate was suspended in normal saline, and then frozen and stored in a refrigerator to obtain a non-growth stationary cell liquid of Nocardiagloberula strain R-9.
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