Globule nocardia strain and use of it for removing organic sulfur in fossil fuel

A technology of Nocardia parvum and fossil fuels, applied in bacteria, petroleum industry, refined hydrocarbon oil, etc., can solve problems such as specific sulfur removal without systematic research, achieve broad industrial application potential, organic resistance The effect of strong solvent ability and strong desulfurization ability

Inactive Publication Date: 2005-04-20
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially for the research on the specific removal of sulfur in sulfur-containing organic compounds in fuels, there has been no systematic research report so far.

Method used

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  • Globule nocardia strain and use of it for removing organic sulfur in fossil fuel
  • Globule nocardia strain and use of it for removing organic sulfur in fossil fuel
  • Globule nocardia strain and use of it for removing organic sulfur in fossil fuel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Screen Nocardia globerula R-9 bacterial strain, its step is as follows:

[0070] Get the aerobic activated sludge sample from the sewage treatment tank of the oil refinery of Dagang Petrochemical Company in China, and take 5 grams of the sample and suspend it in the sulfur-free basic medium (the composition of the medium: 1000ml water, KH 2 PO 4 , 2.44g; Na 2 HPO 4 12H 2 O 14.03g; NH 4 Cl, 2.00g; MgCl 2 ·6H 2 O, 0.36g; CaCl 2 2H 2 O, 1; FeCl 3 1 mg; MnCl 4H 2 O, 4 mg; CuCl 2 , 0.755mg; glycerol 0.8ml or glucose 1-3g, 20 minutes 121 ℃ autoclave), put in a shaker and mix for 30 minutes, let it stand, absorb the upper suspension and transfer it to the nutrient medium (composition g / g: NaCl 1.0%; yeast extract powder 0.5%, tryptone 1%, autoclaved for 20 minutes), put in a shaker and cultivate for 24 hours at 150 rpm. Draw 5ml of the bacterial solution and add it to 100ml of sterilized basal medium, and then add dibenzothiophene DBT-ethanol solution to make the ...

Embodiment 2

[0073] To prepare a liquid culture of cells from the Nocardia globerula R-9 strain:

[0074] Pick a small ball Nocardia globerula R-9 strain cultured on the slope of nutrient agar and inoculate it with a platinum loop in a sterilized test tube, add sterilized DBT-ethanol solution to make the DBT concentration 0.1mmol, and inoculate it at 30 Cultivate in a biological incubator at 150 rpm for 16-24 hours. Take 1ml of the bacterial solution obtained from the culture and inoculate it into a 300ml Erlenmeyer flask equipped with 100ml of basic inorganic salt medium (pH=7.0), and then add sterilized DBT-ethanol solution to make the DBT concentration 0.1-0.5mmol. The Erlenmeyer flask was placed in a biological incubator at 30° C. and cultured at 150 rpm for 48 hours to obtain a liquid culture solution of Nocardia globerula strain R-9 cells.

Embodiment 3

[0076] Preparation of non-growing resting cell fluid of Nocardia globerula strain R-9:

[0077] The liquid culture solution of bacterial strain R-9 cells obtained by the method of Example 2 was centrifuged (8000 rpm) for 10 minutes, and the supernatant suspension was poured off. The centrifuged precipitate was washed with an appropriate amount of normal saline for 3 times, the obtained centrifugal precipitate was suspended in normal saline, and then frozen and stored in a refrigerator to obtain a non-growth stationary cell liquid of Nocardiagloberula strain R-9.

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Abstract

The invention relates to a Nocardia globerula R-9, which was preserved in the "General Microorganism Center of China Microbiological Culture Collection Management Committee" on July 29, 2002, and its preservation number is CGMCC NO.0781. The strain's liquid culture, immobilized cells, resting cells in non-growth period and crude enzyme extract can remove heterocyclic sulfur in sulfur-containing organic compounds through oxidative cleavage of C-S bonds. The strain specifically removes heterocyclic sulfur in fuel without destroying the C-C skeleton of organic compounds, without reducing the combustion calorific value of fuel, and has broad industrial application potential.

Description

technical field [0001] The invention relates to a new strain R-9 of Nocardia globerula and the use of the strain as a catalyst to break the C-S bond in sulfur-containing organic compounds to remove organic sulfur in fossil fuels. Background technique [0002] Petroleum and coal are the most important energy sources in the world. These fossil fuels contain sulfur compounds, which will produce a large amount of sulfur oxides and emit them into the atmosphere when they are burned, forming acid rain, seriously polluting the environment, destroying the ecological balance, and directly threatening the survival of human beings on the earth. living space. According to related reports, nearly 200 million tons of SO2 are discharged into the atmosphere every year in the world, and SO2 in China is 2 The emissions reached 17.95 million tons. At the same time, the existence of sulfide will also affect the appearance of fuel and its products, and corrode combustion and transportation equ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10G32/00C12N1/20C12S1/02
Inventor 姜成英邢建民罗明芳缑仲轩刘会洲安震涛陈家镛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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