Method for separating active blood sugar-lowering substances from silkworm powder

Abstract

The present invention relates to a separation method of blood sugar decreasing active substances from a full silkworm powder, comprising: first, uniformly mixing full silkworm powder and leach buffer solution at the ratio of 1:1, followed by leaching, filtering and separating at the temperature of 4 to 8 degree for extracting blood sugar decreasing active substances from the full silkworm powder, then, carrying out cation exchange column chromatography of full silkworm powder leach buffer solution and adjusting PH value and finally, carrying out eluting, sample collecting, condensing and drying processes. The present invention maintains the original active substances in the silkworm body and has simple blood sugar decreasing component and high yield of 2.7%.

Description

technical field

[0001] The invention relates to a method for separating active substances, in particular to a method for separating blood sugar-lowering active substances in whole silkworm powder. Background technique

[0002] Whole silkworm powder made from silkworm has hypoglycemic effect. Such as the patent "hypoglycemic agent containing silkworm powder as an active ingredient and its manufacturing method (CN95115559.8)", "production method of whole silkworm powder containing lactoferrin (CN03115855.2)", "a whole silkworm powder health food Preparation method (CN03116235.5)", "Mulberry silkworm powder preparation method and its application (CN200410017227.3)". But the silkworm is a eukaryote, an organism with complete life characteristics, and the whole silkworm powder prepared by it has complex components, especially some macromolecular substances such as sugar and fat are contained in the silkworm body, which may be an allergen to some people; During the breeding proc...

Examples

Embodiment Construction

[0014] The separation steps of the present invention are as follows:

[0015] 1. Extraction of active components of whole silkworm powder for lowering blood sugar: mix whole silkworm powder with extraction buffer at a ratio of 1:1, and extract at 4-8°C for 24 hours; then, filter and separate the extraction solution .

[0016] 2. Ion-exchange column chromatography: apply the whole silkworm powder extract to ion-exchange column chromatography, perform cation-exchange column chromatography, and adjust the pH value to be greater than 3.0.

[0017] 3. Elution: water and NH respectively 4 OH was eluted 3-4 times.

[0018] 4. Sample collection: collect 10 ml of samples per tube, and use the method of measuring maltosidase inhibitor activity to detect and collect the active part of glucosidase inhibitor.

[0019] 5. Concentration and drying: freeze-dry the active part of the glucosidase inhibitor at ultra-low temperature and store it for future use.

[0020] The above-mentioned is...

technical field

[0001] The invention relates to a method for separating active substances, in particular to a method for separating blood sugar-lowering active substances in whole silkworm powder. Background technique

[0002] Whole silkworm powder made from silkworm has hypoglycemic effect. Such as the patent "hypoglycemic agent containing silkworm powder as an active ingredient and its manufacturing method (CN95115559.8)", "production method of whole silkworm powder containing lactoferrin (CN03115855.2)", "a whole silkworm powder health food Preparation method (CN03116235.5)", "Mulberry silkworm powder preparation method and its application (CN200410017227.3)". But the silkworm is a eukaryote, an organism with complete life characteristics, and the whole silkworm powder prepared by it has complex components, especially some macromolecular substances such as sugar and fat are contained in the silkworm body, which may be an allergen to some people; During the breeding proc...

Examples

Embodiment Construction

[0014] The separation steps of the present invention are as follows:

[0015] 1. Extraction of active components of whole silkworm powder for lowering blood sugar: mix whole silkworm powder with extraction buffer at a ratio of 1:1, and extract at 4-8°C for 24 hours; then, filter and separate the extraction solution .

[0016] 2. Ion-exchange column chromatography: apply the whole silkworm powder extract to ion-exchange column chromatography, perform cation-exchange column chromatography, and adjust the pH value to be greater than 3.0.

[0017] 3. Elution: water and NH respectively 4 OH was eluted 3-4 times.

[0018] 4. Sample collection: collect 10 ml of samples per tube, and use the method of measuring maltosidase inhibitor activity to detect and collect the active part of glucosidase inhibitor.

[0019] 5. Concentration and drying: freeze-dry the active part of the glucosidase inhibitor at ultra-low temperature and store it for future use.

[0020] The above-mentioned is...