Capillary electrophoresis method for detecting stilbene glucoside and anthraquinone component in polygonum multiflorum

Abstract

The invention belongs to the technical field of analysis and detection, and discloses a capillary electrophoresis method for simultaneously separating and detecting stilbene glucoside and anthraquinone component in polygonum multiflorum. The method adopts micellar electrokinetic capillary chromatography to analyze and detect polygonum multiflorum extracting solution to obtain the electrophoresis pattern of stilbene glucoside, emodin, aloeemodin, rhein, chrysophanol or physcion; according to a correction curvilinear equation, the concentration of stilbene glucoside, emodin, aloeemodin, rhein, chrysophanol or physcion is calculated. The invention can simultaneously analyze and detect the stilbene glucoside and anthraquinone component in polygonum multiflorum and is simple, quick and efficient; adopted buffer liquid is mainly aqueous solution, does not need a great quantity of organic solvent and has small pollution and environment protection; a small amount of needed solvent and sample is needed, and analysis cost is low.

Description

technical field

[0001] The invention belongs to the technical field of analysis and detection, in particular to a capillary electrophoresis method for simultaneously detecting stilbene glycosides and anthraquinone components in Polygonum multiflorum. Background technique

[0002] Capillary electrophoresis is a new separation and analysis technique emerging since the 1980s. It has the advantages of high resolution, short analysis time, less consumption of solvent and sample, simple sample pretreatment, and simultaneous separation and determination of multi-component samples. It is widely used in drug analysis and detection.

[0003] The traditional Chinese medicine Polygonum multiflorum is the dry tuber root of Polygonum multiflorum, which is mainly produced in Guangdong, Sichuan, Hunan, Guizhou and other places in my country. Studies have shown that the main active ingredients of Polygonum multiflorum are stilbene glycosides and anthraquinones. At present, the Chinese Phar...

Examples

Embodiment 1

[0024] 1) Determination of the best detection conditions:

[0025] (1) Preparation of electrophoresis buffer:

[0026] Prepare respectively the borax and sodium lauryl sulfate mother liquor that concentration is 100mmol / L with deionized water;

[0027] a. Utilize the borax and sodium lauryl sulfate mother liquor with a concentration of 100mmol / L to prepare 15mmol of sodium lauryl sulfate concentrations of 10mmol / L, 20mmol / L, 30mmol / L, 40mmol / L and 50mmol / L / L borax-sodium lauryl sulfate-volume ratio 10% methanol buffer solution, with 5mmol / L sodium hydroxide solution and hydrochloric acid to adjust the pH to 9.6;

[0028] B, utilize concentration to be the borax of 100mmol / L and sodium lauryl sulfate mother liquor, preparation borax concentration is respectively the borax-30mmol / L ten of 5mmol / L, 10mmol / L, 15mmol / L, 20mmol / L and 25mmol / L Dialkyl sodium sulfate-volume ratio 10% methanol buffer, with 5mmol / L sodium hydroxide solution and hydrochloric acid to adjust the pH to 9...

Embodiment 2

[0051] For the analysis of stilbene glycosides and anthraquinones in Polygonum multiflorum, which is produced in Deqing, Guangdong, the instruments and solution processing methods used are as described above, and the detection steps are as follows:

[0052] (1) Preparation of extract

[0053] Crush the dried roots of Radix Polygoni Multiflori, pass through a 100-mesh sieve, take 0.6g of the powder, add 50mL of methanol, ultrasonically extract with methanol for 20min, and filter to obtain the filtrate and filter residue; wash the filter residue with 10mL of methanol for 3 times, and combine the washing liquid with the above-mentioned filtrate After removing the solvent by evaporating under reduced pressure, add 5 mL of methanol to dissolve, shake well, and filter through a 0.22 μm filter membrane to obtain the sample extract of Polygonum multiflorum.

[0054] (2) Preparation of 15mmol / L borax-30mmol / L sodium lauryl sulfate-10% methanol by volume, pH 9.6 buffer solution

[0055...

Embodiment 3

[0062] For the analysis of stilbene glycosides and anthraquinones in Radix Polygoni Multiflori Polygonum multiflorum, which is produced in Mount Emei, Sichuan, the instruments and solution processing methods used are as described above, and the detection steps are as follows:

[0063] (1) Preparation of extract

[0064] Crush the dried root tubers of Emeishan Polygonum multiflorum, pass through a 120-mesh sieve, take 1.0 g of the powder, add 60 mL of methanol, use methanol for ultrasonic extraction for 30 minutes, and filter to obtain the filtrate and filter residue; wash the filter residue with 15 mL of methanol for 4 times, and mix the washing liquid and the above-mentioned filtrate Combined, evaporated under reduced pressure to remove the solvent, then added 5 mL of methanol to dissolve, shake well, and filter through a 0.22 μm filter membrane to obtain the sample extract of Polygonum multiflorum.

[0065] (2) 15mmol / L borax-30mmol / L sodium lauryl sulfate-10% methanol (volu...

technical field

[0001] The invention belongs to the technical field of analysis and detection, in particular to a capillary electrophoresis method for simultaneously detecting stilbene glycosides and anthraquinone components in Polygonum multiflorum. Background technique

[0002] Capillary electrophoresis is a new separation and analysis technique emerging since the 1980s. It has the advantages of high resolution, short analysis time, less consumption of solvent and sample, simple sample pretreatment, and simultaneous separation and determination of multi-component samples. It is widely used in drug analysis and detection.

[0003] The traditional Chinese medicine Polygonum multiflorum is the dry tuber root of Polygonum multiflorum, which is mainly produced in Guangdong, Sichuan, Hunan, Guizhou and other places in my country. Studies have shown that the main active ingredients of Polygonum multiflorum are stilbene glycosides and anthraquinones. At present, the Chinese Phar...

Examples

Embodiment 1

[0024] 1) Determination of the best detection conditions:

[0025] (1) Preparation of electrophoresis buffer:

[0026] Prepare respectively the borax and sodium lauryl sulfate mother liquor that concentration is 100mmol / L with deionized water;

[0027] a. Utilize the borax and sodium lauryl sulfate mother liquor with a concentration of 100mmol / L to prepare 15mmol of sodium lauryl sulfate concentrations of 10mmol / L, 20mmol / L, 30mmol / L, 40mmol / L and 50mmol / L / L borax-sodium lauryl sulfate-volume ratio 10% methanol buffer solution, with 5mmol / L sodium hydroxide solution and hydrochloric acid to adjust the pH to 9.6;

[0028] B, utilize concentration to be the borax of 100mmol / L and sodium lauryl sulfate mother liquor, preparation borax concentration is respectively the borax-30mmol / L ten of 5mmol / L, 10mmol / L, 15mmol / L, 20mmol / L and 25mmol / L Dialkyl sodium sulfate-volume ratio 10% methanol buffer, with 5mmol / L sodium hydroxide solution and hydrochloric acid to adjust the pH to 9...

Embodiment 2

[0051] For the analysis of stilbene glycosides and anthraquinones in Polygonum multiflorum, which is produced in Deqing, Guangdong, the instruments and solution processing methods used are as described above, and the detection steps are as follows:

[0052] (1) Preparation of extract

[0053] Crush the dried roots of Radix Polygoni Multiflori, pass through a 100-mesh sieve, take 0.6g of the powder, add 50mL of methanol, ultrasonically extract with methanol for 20min, and filter to obtain the filtrate and filter residue; wash the filter residue with 10mL of methanol for 3 times, and combine the washing liquid with the above-mentioned filtrate After removing the solvent by evaporating under reduced pressure, add 5 mL of methanol to dissolve, shake well, and filter through a 0.22 μm filter membrane to obtain the sample extract of Polygonum multiflorum.

[0054] (2) Preparation of 15mmol / L borax-30mmol / L sodium lauryl sulfate-10% methanol by volume, pH 9.6 buffer solution

[0055...

Embodiment 3

[0062] For the analysis of stilbene glycosides and anthraquinones in Radix Polygoni Multiflori Polygonum multiflorum, which is produced in Mount Emei, Sichuan, the instruments and solution processing methods used are as described above, and the detection steps are as follows:

[0063] (1) Preparation of extract

[0064] Crush the dried root tubers of Emeishan Polygonum multiflorum, pass through a 120-mesh sieve, take 1.0 g of the powder, add 60 mL of methanol, use methanol for ultrasonic extraction for 30 minutes, and filter to obtain the filtrate and filter residue; wash the filter residue with 15 mL of methanol for 4 times, and mix the washing liquid and the above-mentioned filtrate Combined, evaporated under reduced pressure to remove the solvent, then added 5 mL of methanol to dissolve, shake well, and filter through a 0.22 μm filter membrane to obtain the sample extract of Polygonum multiflorum.

[0065] (2) 15mmol / L borax-30mmol / L sodium lauryl sulfate-10% methanol (volu...

Images