Preparation method of microbial fertilizer

Abstract

The invention provides a preparation method of microbial fertilizer. The method comprises the following steps: (a) regulating pH value of biomass power plant ash; (b) fermenting microbial strains; and (c) mixing the fermented microbial strain liquid and the pH value regulated biomass power plant ash in proportion. The preparation method of microbial fertilizer in the invention adopts biomass power plant ash as fundamental carrier to prepare microbial fertilizer, thus efficiently utilizing the ash discharged by biomass power plant; doped bacteria content in biomass power plant ash is low under the prerequisite that ash discharging process is controlled, thus sterilization process is omitted and resource is centralized. The invention efficiently broadens raw material range for microbial fertilizer and reduces cost of microbial fertilizer. The method enhances utilization value of power plant ash and alleviates pollution to the environment caused by biomass power plant ash.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, in particular to a production method for making microbial fertilizer.

Background technique

[0002] At present, there are about 300 domestic enterprises engaged in the production of microbial fertilizers, most of which use raw materials such as straw, livestock manure, peat and crop shell residue as carriers, and add beneficial microorganisms to them.

[0003] However, the microorganisms produced by this method generally have the following core problems:

[0004] One is that the number of effective bacteria is too small, and most of the products produced by the companies do not meet the industry standard NY227-94 of the Ministry of Agriculture, that is, the compound microbial fertilizer contains 200 million live bacteria / g, and the rate of bacteria is less than 20%;

[0005] The second is that the miscellaneous bacteria seriously exceed the standard, the main reason is that there is no effec...

Examples

no. 1 example

[0020] The first embodiment: Using biomass power plant ash to prepare nitrogen-fixing bacteria fertilizer

[0021] The first embodiment of the present invention uses biomass power plant ash to prepare nitrogen-fixing bacteria fertilizer. The process of preparing nitrogen-fixing bacteria fertilizer from biomass power plant ash is described in detail below through method one and method two respectively.

[0022] method one:

[0023] (1) Fermentation nitrogen-fixing strains;

[0024] After activation of nitrogen-fixing bacteria, transfer to sterilized LB or YPGA liquid seed culture medium, adjust pH 7.0-7.5, incubate at 30℃-32℃ for 12-16 hours, and then transfer to killed according to 1%-5% inoculum Bacteria in a large tank of culture medium. In a large tank culture medium, culture at 30℃-32℃ for 14-18 hours, other fermentation conditions are operated according to conventional techniques, and the number of bacteria reaches 5-10×10 9 CFU / ml. See Table 1 for the formulation of the large...

no. 2 example

[0039] Second embodiment: Using biomass power plant ash to prepare compound microbial fertilizer

[0040] The first embodiment of the present invention uses biomass power plant ash to prepare compound microbial fertilizer. The process of preparing composite microbial fertilizer using biomass power plant ash will be described in detail below.

[0041] (1) Fermentation strains;

[0042] The Azotobacter, Bacillus subtilis, Phosphobacteria and Bacillus licheniformis that are to be produced are activated separately and transferred to LB or YPGA liquid seed culture medium, and potassium bacteria are activated and transferred to potassium bacteria culture medium. After culturing at 30°C-37°C for 12-16 hours, they are then transferred to a large tank medium at 1%-5% inoculum. The culture temperature and time were 16 hours for nitrogen-fixing bacteria at 30°C, 36 hours for Bacillus subtilis at 30°C, 32 hours for phosphorus bacteria at 32°C, 32 hours for Bacillus licheniformis at 37°C, and 3...

Technical Field

[0001] The present invention relates to the field of microbial technology, in particular to a production method for making microbial fertilizer.

Background technique

[0002] At present, there are about 300 domestic enterprises engaged in the production of microbial fertilizers, most of which use raw materials such as straw, livestock manure, peat and crop shell residue as carriers, and add beneficial microorganisms to them.

[0003] However, the microorganisms produced by this method generally have the following core problems:

[0004] One is that the number of effective bacteria is too small, and most of the products produced by the companies do not meet the industry standard NY227-94 of the Ministry of Agriculture, that is, the compound microbial fertilizer contains 200 million live bacteria / g, and the rate of bacteria is less than 20%;

[0005] The second is that the miscellaneous bacteria seriously exceed the standard, the main reason is that there is no effec...

Examples

no. 1 example

[0020] The first embodiment: Using biomass power plant ash to prepare nitrogen-fixing bacteria fertilizer

[0021] The first embodiment of the present invention uses biomass power plant ash to prepare nitrogen-fixing bacteria fertilizer. The process of preparing nitrogen-fixing bacteria fertilizer from biomass power plant ash is described in detail below through method one and method two respectively.

[0022] method one:

[0023] (1) Fermentation nitrogen-fixing strains;

[0024] After activation of nitrogen-fixing bacteria, transfer to sterilized LB or YPGA liquid seed culture medium, adjust pH 7.0-7.5, incubate at 30℃-32℃ for 12-16 hours, and then transfer to killed according to 1%-5% inoculum Bacteria in a large tank of culture medium. In a large tank culture medium, culture at 30℃-32℃ for 14-18 hours, other fermentation conditions are operated according to conventional techniques, and the number of bacteria reaches 5-10×10 9 CFU / ml. See Table 1 for the formulation of the large...

no. 2 example

[0039] Second embodiment: Using biomass power plant ash to prepare compound microbial fertilizer

[0040] The first embodiment of the present invention uses biomass power plant ash to prepare compound microbial fertilizer. The process of preparing composite microbial fertilizer using biomass power plant ash will be described in detail below.

[0041] (1) Fermentation strains;

[0042] The Azotobacter, Bacillus subtilis, Phosphobacteria and Bacillus licheniformis that are to be produced are activated separately and transferred to LB or YPGA liquid seed culture medium, and potassium bacteria are activated and transferred to potassium bacteria culture medium. After culturing at 30°C-37°C for 12-16 hours, they are then transferred to a large tank medium at 1%-5% inoculum. The culture temperature and time were 16 hours for nitrogen-fixing bacteria at 30°C, 36 hours for Bacillus subtilis at 30°C, 32 hours for phosphorus bacteria at 32°C, 32 hours for Bacillus licheniformis at 37°C, and 3...

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