Method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings

Abstract

The invention provides a method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings, which comprises the following steps of: performing artificial pollination to obtain hybrid seeds; picking off the capsule when the capsule has not reached full maturity; disinfecting the surface of the capsule and aseptically sowing the capsule; culturing the capsule in the dark and sprouting to obtain the rhizome; inoculating the rhizome into a bud induction culture medium; inducing bud through the rhizome, rooting and culturing the sprout; and transplanting test-tube plantletand the like. The method ensures high germination of the cymbidium goeringii hybrid seeds, short period for raising the seedlings and good quality of the seedlings by using three culture media optimized by sifting. The complete set of method of the invention has high operability, well solves the problems of extremely difficult germination of the cymbidium goeringii hybrid seeds under natural conditions due to the incomplete embryonic development and has high application value and popularization value.

Description

technical field

[0001] The invention belongs to the field of plant biotechnology, in particular to a method for hybrid breeding and seed propagation of Chunlan. Background technique

[0002] Orchid is a traditional characteristic flower in China. Chinese orchid usually refers to a part of the ground species in the Orchidaceae (Orchidaceae) of the genus Cymbidum, such as Chunlan (C.goeringii), Cymbidium (C.faberi), Jianlan (C. ensifolium), Molan (C.sinense) and Hanlan (C.kanran), etc. Orchids are elegant and fragrant, highly praised in poems and paintings, and known as "a gentleman among flowers". Confucian culture advocates "a gentleman is better than virtue", and flowers are often used as metaphors, making orchids very popular in Southeast Asian countries. The quantity of fine orchid varieties is small and the price is high, and new varieties are mainly obtained by introduction and domestication. Since the 1980s, various places have taken advantage of local resources to ca...

Examples

Embodiment 1

[0038] Hybridization of Chunlan Dafugui and Lvyun and aseptic sowing of seeds:

[0039] (1) Artificial pollination: In the middle and late February, when the two parents bloom at the same time, they can be the parents of each other, and carry out reciprocal crossing. Before and after pollination, care must be taken to prevent the influence of insects. Remove the pollen pieces and exchange them with each other, then pick up the pollen pieces of the male parent with tweezers and carefully place them in the groove of the stamen column of the female parent. Pay attention to the lightness of the gesture to prevent the tip of the tweezers from hitting the wall of the stamen column. 1 flower The pollen block can confer 2 to 4 flowers. After pollination, cover the stamen with a small piece of white non-woven fabric or cover the whole flower with a pollination bag made of translucent paper. 1. Pollination date and pollinator, check the pollination expansion after 15-30 days, if the pol...

Embodiment 2

[0045] Chunlan Jiyuan (♀) and Yuanhong Methods of hybridization and aseptic sowing of seeds:

[0046] (1) Artificial pollination: the flowering period of Yuanhong is 20 days earlier than Jiyuan, remove the pollen block of Yuanhong, put it in a small plastic ziplock bag, and store it in a dry (silica gel) low temperature (5°C) refrigerator. When Jiyuan blooms, take out the refrigerated pollen blocks and place them on white paper, and place them in an environment with a suitable temperature and humidity (temperature 20-25°C, humidity 70-75%) for 30 minutes, and individual samples are tested by TTC staining method. Test the vitality of the pollen blocks, and pollinate the remaining pollen blocks on the round stamens. After pollination, cover the stamens with a small piece of white non-woven fabric or cover the whole flower with a pollination bag made of translucent paper. Flowers, hang a plastic card, write clearly the parents, pollination date and pollinator, check the pollina...

technical field

[0001] The invention belongs to the field of plant biotechnology, in particular to a method for hybrid breeding and seed propagation of Chunlan. Background technique

[0002] Orchid is a traditional characteristic flower in China. Chinese orchid usually refers to a part of the ground species in the Orchidaceae (Orchidaceae) of the genus Cymbidum, such as Chunlan (C.goeringii), Cymbidium (C.faberi), Jianlan (C. ensifolium), Molan (C.sinense) and Hanlan (C.kanran), etc. Orchids are elegant and fragrant, highly praised in poems and paintings, and known as "a gentleman among flowers". Confucian culture advocates "a gentleman is better than virtue", and flowers are often used as metaphors, making orchids very popular in Southeast Asian countries. The quantity of fine orchid varieties is small and the price is high, and new varieties are mainly obtained by introduction and domestication. Since the 1980s, various places have taken advantage of local resources to ca...

Examples

Embodiment 1

[0038] Hybridization of Chunlan Dafugui and Lvyun and aseptic sowing of seeds:

[0039] (1) Artificial pollination: In the middle and late February, when the two parents bloom at the same time, they can be the parents of each other, and carry out reciprocal crossing. Before and after pollination, care must be taken to prevent the influence of insects. Remove the pollen pieces and exchange them with each other, then pick up the pollen pieces of the male parent with tweezers and carefully place them in the groove of the stamen column of the female parent. Pay attention to the lightness of the gesture to prevent the tip of the tweezers from hitting the wall of the stamen column. 1 flower The pollen block can confer 2 to 4 flowers. After pollination, cover the stamen with a small piece of white non-woven fabric or cover the whole flower with a pollination bag made of translucent paper. 1. Pollination date and pollinator, check the pollination expansion after 15-30 days, if the pol...

Embodiment 2

[0045] Chunlan Jiyuan (♀) and Yuanhong Methods of hybridization and aseptic sowing of seeds:

[0046] (1) Artificial pollination: the flowering period of Yuanhong is 20 days earlier than Jiyuan, remove the pollen block of Yuanhong, put it in a small plastic ziplock bag, and store it in a dry (silica gel) low temperature (5°C) refrigerator. When Jiyuan blooms, take out the refrigerated pollen blocks and place them on white paper, and place them in an environment with a suitable temperature and humidity (temperature 20-25°C, humidity 70-75%) for 30 minutes, and individual samples are tested by TTC staining method. Test the vitality of the pollen blocks, and pollinate the remaining pollen blocks on the round stamens. After pollination, cover the stamens with a small piece of white non-woven fabric or cover the whole flower with a pollination bag made of translucent paper. Flowers, hang a plastic card, write clearly the parents, pollination date and pollinator, check the pollina...

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