Effective parts of the wind-chasing umbrella and its extraction method and application

Abstract

The invention relates to an active part of lysimachia trientaloides Hemsl.. The active part of the lysimachia trientaloides Hemsl. may be one or any combination of a petroleum ether part, an ethyl acetate part or an n-butyl alcohol part of the lysimachia trientaloides Hemsl. extracts. The extraction method particularly comprises the following steps of: lixiviating dry lysimachia trientaloides Hemsl. powder with methanol or ethanol aqueous solution; concentrating the immersion liquid to obtain an extractum; dispersing the extractum into distilled water; extracting by using the solvents of petroleum ether, ethyl acetate part and n-butyl alcohol sequentially for three times respectively; and volatilizing the solvents to obtain the petroleum ether part, the ethyl acetate part and the n-butyl alcohol part respectively. The active part of the lysimachia trientaloides Hemsl. has high inhibition activity on alpha-glucosidase and good curative effect on the mesoxyalyurea induced diabetes mice,and can be used for preparing medicines for reducing blood sugar.

Description

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an extract of a plant chasing umbrella, an extracting method thereof, and an application thereof in preparing hypoglycemic drugs. Background technique

[0002] chasing umbrella ( Lysimachia paridiformis Franch. var. Stenophylla Franch.) alias Jingfeng umbrella, an umbrella, male jiegudan, broken parasol, back flower grass, lampstand grass, umbrella leaf row grass, is the whole plant or root of the Primula genus Prunus angustifolia, distributed In Guizhou, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Yunnan and other places. Its taste is pungent, spicy, bitter, hot in nature. Enter the Leng Jing. The main functions are dispelling wind and dredging collaterals, promoting blood circulation and relieving pain; mainly treating rheumatic arthralgia, spasms of limbs, hemiplegia, convulsions in children, falling down, fractures and other diseases. ...

Examples

Embodiment 1

[0019] An effective part of the umbrella, which is selected from one or any combination of the petroleum ether part, the ethyl acetate part or the n-butanol part of the ethanol extract of the umbrella; the specific extraction method is: the whole herb of the umbrella Dry in the shade and pulverize, take 1000 g of dried wind-seeking umbrella powder, soak it 3 times at room temperature with methanol aqueous solution with a volume fraction of 70% (the amount of methanol aqueous solution added is 3-5 times the weight of the wind-chasing umbrella powder), 3 times each time day, combined the obtained extracts three times and concentrated them into extracts; the extracts were dispersed in 1000ml distilled water, and then extracted with solvent petroleum ether (3000ml), ethyl acetate (3000ml) and n-butanol (3000ml) successively, each extraction Three times; after evaporating the solvent, the solid petroleum ether part (LPFPE), ethyl acetate part (LPFEA) and n-butanol part (LPFBU) were ...

Embodiment 2

[0022] Replace the methanol aqueous solution with volume fraction 70% with the ethanol aqueous solution of volume fraction 70%, other is with embodiment 1, obtain petroleum ether part (LPFPE), ethyl acetate part (LPFEA) and n-butanol part (LPFBU), extraction rate is respectively 1.5%, 5.8% and 2.2%.

[0023] Effect test

[0024] The petroleum ether part (LPFPE), the ethyl acetate part (LPFEA) and the n-butanol part (LPFBU) that the embodiment 1 selects 70% methanol aqueous solution and the embodiment 2 selects 70% ethanol aqueous alcohol extraction to obtain respectively below carry out the reduction Results of blood sugar test.

[0025] Test 1. In vitro enzyme inhibitory activity test of the effective part of the umbrella

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an extract of a plant chasing umbrella, an extracting method thereof, and an application thereof in preparing hypoglycemic drugs. Background technique

[0002] chasing umbrella ( Lysimachia paridiformis Franch. var. Stenophylla Franch.) alias Jingfeng umbrella, an umbrella, male jiegudan, broken parasol, back flower grass, lampstand grass, umbrella leaf row grass, is the whole plant or root of the Primula genus Prunus angustifolia, distributed In Guizhou, Hubei, Hunan, Guangdong, Guangxi, Sichuan, Yunnan and other places. Its taste is pungent, spicy, bitter, hot in nature. Enter the Leng Jing. The main functions are dispelling wind and dredging collaterals, promoting blood circulation and relieving pain; mainly treating rheumatic arthralgia, spasms of limbs, hemiplegia, convulsions in children, falling down, fractures and other diseases. ...

Examples

Embodiment 1

[0019] An effective part of the umbrella, which is selected from one or any combination of the petroleum ether part, the ethyl acetate part or the n-butanol part of the ethanol extract of the umbrella; the specific extraction method is: the whole herb of the umbrella Dry in the shade and pulverize, take 1000 g of dried wind-seeking umbrella powder, soak it 3 times at room temperature with methanol aqueous solution with a volume fraction of 70% (the amount of methanol aqueous solution added is 3-5 times the weight of the wind-chasing umbrella powder), 3 times each time day, combined the obtained extracts three times and concentrated them into extracts; the extracts were dispersed in 1000ml distilled water, and then extracted with solvent petroleum ether (3000ml), ethyl acetate (3000ml) and n-butanol (3000ml) successively, each extraction Three times; after evaporating the solvent, the solid petroleum ether part (LPFPE), ethyl acetate part (LPFEA) and n-butanol part (LPFBU) were ...

Embodiment 2

[0022] Replace the methanol aqueous solution with volume fraction 70% with the ethanol aqueous solution of volume fraction 70%, other is with embodiment 1, obtain petroleum ether part (LPFPE), ethyl acetate part (LPFEA) and n-butanol part (LPFBU), extraction rate is respectively 1.5%, 5.8% and 2.2%.

[0023] Effect test

[0024] The petroleum ether part (LPFPE), the ethyl acetate part (LPFEA) and the n-butanol part (LPFBU) that the embodiment 1 selects 70% methanol aqueous solution and the embodiment 2 selects 70% ethanol aqueous alcohol extraction to obtain respectively below carry out the reduction Results of blood sugar test.

[0025] Test 1. In vitro enzyme inhibitory activity test of the effective part of the umbrella

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