Preparation method of CuS nanoparticles, product and application thereof

Abstract

The invention discloses a method for preparing CuS nanoparticles, which comprises the following steps: S1. preparation of copper-sulfhydryl complex solution, namely mixing copper salt and sulfhydryl polypeptide in an aqueous solution to form the copper-sulfhydryl complex solution, wherein the mol ratio of copper to sulfhydryl polypeptide is (0.1-6):1, and the concentration of copper in the copper-sulfhydryl complex solution is 0.0003-0.01 mol / L; and S2. preparation of sulfhydryl-modified CuS nanoparticles: regulating the pH value of the copper-sulfhydryl complex solution to 7-12, adding a sulfur source, reacting at room temperature for 6-12 hours to obtain the sulfhydryl-modified CuS nanoparticle solution, wherein the mol ratio of copper to sulfur source is 1:(0.5-15). The invention also discloses CuS nanoparticles prepared by the method, and application of the CuS nanoparticles in photo-thermal therapy and opto-acoustic imaging. The preparation method disclosed by the invention has the advantage of mild conditions, and is simple to operate; and the prepared nanoparticles have the advantages of small size and favorable dispersity, and have strong opto-acoustic signals and excellent photo-thermal conversion capacity.

Description

Technical field

[0001] The invention relates to the field of nano functional materials, in particular to a preparation method of copper sulfide nanoparticles, products and applications thereof.

Background technique

[0002] Nanomaterials, especially nanostructures with special morphology constructed of one-dimensional and two-dimensional nanomaterials, have attracted scientific and technological work in recent years due to their excellent optical, electrical, catalytic and other properties and potential applications in nanodevices. extensive attention of the audience.

[0003] Among them, CuS is a multifunctional vulcanization synthetic material with good chemical stability and an important semiconductor material. Nano-CuS has a small particle size and a large specific surface area. The semiconductor nanocrystal acts as a medium for conducting electrons between molecular entities and microcrystals. This semiconductor material has potential in the preparation of light-em...

Examples

preparation example Construction

[0034] The preparation method of the present invention may also include a purification step S3: for example, dialysis may be used for purification operation, at room temperature, sequentially dialyze the solution of sulfhydryl-modified CuS nanoparticles using alkaline buffer solution and redistilled water to remove small inorganic molecules. The alkaline buffer of this step can use phosphate buffered saline (PBS), especially the phosphate buffered saline that pH is 9, or other alkaline buffers.

[0035] The solution of the finally obtained sulfhydryl-modified CuS nanoparticles can be stored lyophilized at 4° C., so that it can be stored for a longer period of time.

[0036] The preparation method of the invention is carried out at room temperature, and the reaction conditions are mild; the operation is simple and the reproducibility is good; the biomolecular synthesis is adopted, and the cytotoxicity of the nanoparticles can be reduced.

[0037] The thus prepared sulfhydryl-mo...

Embodiment 1

[0043] Add 3mL of 0.1mol / L glutathione solution to 1.5ml of 0.1mol / L copper chloride solution, mix well, and add 25ml of water.

[0044] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.

[0045] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer solution (pH 9) for 24 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 12 hours.

[0046] The dialysate was lyophilized and stored at 4°C.

Embodiment 2

[0048] Add 0.5mL 0.1mol / L glutathione solution to 3ml 0.1mol / L copper sulfate solution, mix well, and add 25ml water.

[0049] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.

[0050] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer (pH 9) for 72 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 6 hours.

[0051] The dialysate was lyophilized and stored at 4°C.

Technical field

[0001] The invention relates to the field of nano functional materials, in particular to a preparation method of copper sulfide nanoparticles, products and applications thereof.

Background technique

[0002] Nanomaterials, especially nanostructures with special morphology constructed of one-dimensional and two-dimensional nanomaterials, have attracted scientific and technological work in recent years due to their excellent optical, electrical, catalytic and other properties and potential applications in nanodevices. extensive attention of the audience.

[0003] Among them, CuS is a multifunctional vulcanization synthetic material with good chemical stability and an important semiconductor material. Nano-CuS has a small particle size and a large specific surface area. The semiconductor nanocrystal acts as a medium for conducting electrons between molecular entities and microcrystals. This semiconductor material has potential in the preparation of light-em...

Examples

preparation example Construction

[0034] The preparation method of the present invention may also include a purification step S3: for example, dialysis may be used for purification operation, at room temperature, sequentially dialyze the solution of sulfhydryl-modified CuS nanoparticles using alkaline buffer solution and redistilled water to remove small inorganic molecules. The alkaline buffer of this step can use phosphate buffered saline (PBS), especially the phosphate buffered saline that pH is 9, or other alkaline buffers.

[0035] The solution of the finally obtained sulfhydryl-modified CuS nanoparticles can be stored lyophilized at 4° C., so that it can be stored for a longer period of time.

[0036] The preparation method of the invention is carried out at room temperature, and the reaction conditions are mild; the operation is simple and the reproducibility is good; the biomolecular synthesis is adopted, and the cytotoxicity of the nanoparticles can be reduced.

[0037] The thus prepared sulfhydryl-mo...

Embodiment 1

[0043] Add 3mL of 0.1mol / L glutathione solution to 1.5ml of 0.1mol / L copper chloride solution, mix well, and add 25ml of water.

[0044] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.

[0045] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer solution (pH 9) for 24 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 12 hours.

[0046] The dialysate was lyophilized and stored at 4°C.

Embodiment 2

[0048] Add 0.5mL 0.1mol / L glutathione solution to 3ml 0.1mol / L copper sulfate solution, mix well, and add 25ml water.

[0049] Use 1mol / L NaOH solution to adjust the pH to 9, add 1.5mL 0.1mol / L thioacetamide, and shake gently at room temperature for 12h.

[0050] Separation of small inorganic molecules by dialysis: transfer the final product of the reaction into a dialysis bag. At room temperature, dialyze in 500ml PBS buffer (pH 9) for 72 hours, change the medium once every 12 hours, and then dialyze in 500ml double distilled water for 6 hours.

[0051] The dialysate was lyophilized and stored at 4°C.

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