Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea

Abstract

The invention discloses a Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea, and belongs to the field of feed additives. The feed additive is prepared from the main materials of snow grass meal, curcuma powder, semen cassiae powder, burdock powder, shallot white powder, Chinese herbal medicine preparation and curcuma extract, and toadstool and bacillus subtilis are scientifically compounded, so that the application amount of antibiotics medicine in cultivation of pigs is effectively reduced, the use amount of the antibiotic is reduced by above 60%, the drug property of the Chinese herbal medicine is improved, the porcine epizootic diarrhea caused by porcine epidemic diarrhea virus can be effectively prevented and treated, and the Chinese herb extract feed additive is safe and non-toxic. Besides, the amount of the additive in the feed is low, the quality is stable, an acting effect is improved by combining different Chinese herb extracts, the porcine epizootic diarrhea is reduced by 60-80%, and the taste and quality of animal products are obviously improved. A sense experimental evaluation proves that the evaluation score on the taste and quality of the animal products using the additive is improved by above 65%.

Description

Technical field:

[0001] The invention belongs to the field of feed additives, in particular to a Chinese herbal medicine extract feed additive which can effectively prevent and treat porcine epidemic diarrhea. Background technique:

[0002] Porcine epidemic diarrhea (porcine epidemic diarrhea, PED) is caused by porcine epidemic diarrhea virus (porcine epidemic diarrhea virus, PEDV) and is characterized by watery diarrhea in pigs, vomiting, dehydration and high lethality to suckling piglets. Highly contagious enteric infectious disease. Porcine epidemic diarrhea virus belongs to the genus Coronaviridae of the family Coronaviridae. Virus particles are pleomorphic, tend to be spherical, and have a diameter of 95-l90nm. There is a capsule outside, and there are petal-like fibrous protrusions on the capsule. The nucleic acid type of porcine epidemic diarrhea virus is RNA type. In 1995, the sixth report of the International Committee on Taxonomy of Viruses (CTV) listed PEDV as ...

Examples

preparation example Construction

[0035] Preparation of Bacillus subtilis bacterial agent:

[0036] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;

[0037] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;

[0038] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum amount into a 5000 ml tertiary seed shaker flask, with a medium capacity of 1000 ml, a rotary shaker at 100 rpm, and a culture temperature of 30°C. Time 24 hours;

[0039] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with a total volume of 150L with 10% inoculation amount, the fermentation medium capacity is 100L, the cul...

Embodiment 1

[0066] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:

[0067] Centella asiatica powder 10 parts; turmeric powder 5 parts; cassia seed powder 8 parts; burdock seed powder 10 parts; 5 parts of bacterial agent.

[0068] The preparation method of described Chinese herbal medicine preparation is as follows:

[0069] Accurately weigh 20 parts of Centella asiatica; 10 parts of cassia seed; 5 parts of arctium; Keep the temperature at 70°C for 2 hours, add a mixture of ethanol and propanol twice the weight of the mixed material, control the temperature to 60°C, keep it for 3 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.

[0070] The mass ratio of ethanol and propanol is 1:1; the ethanol concentration is 95%; the propanol concentration is 100%;

[0071] The preparation ...

Embodiment 2

[0078] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:

[0079] 15 parts of centella asiatica powder; 10 parts of turmeric powder; 15 parts of cassia seed powder; 20 parts of burdock seed powder; 10 parts of bacterial agent.

[0080] The preparation method of described Chinese herbal medicine preparation is as follows:

[0081] Accurately weigh 25 parts of Centella Asiatica; 18 parts of Semen Cassiae; 15 parts of Arctium; Keep the temperature at 90°C for 4 hours, add a mixture of ethanol and propanol 3 times the weight of the mixed material, control the temperature to 78°C, keep it for 4 hours, filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.

[0082] The mass ratio of described ethanol and propanol is 1:1.5, and ethanol concentration is 95%; Propanol concentration is 100%;

...

Technical field:

[0001] The invention belongs to the field of feed additives, in particular to a Chinese herbal medicine extract feed additive which can effectively prevent and treat porcine epidemic diarrhea. Background technique:

[0002] Porcine epidemic diarrhea (porcine epidemic diarrhea, PED) is caused by porcine epidemic diarrhea virus (porcine epidemic diarrhea virus, PEDV) and is characterized by watery diarrhea in pigs, vomiting, dehydration and high lethality to suckling piglets. Highly contagious enteric infectious disease. Porcine epidemic diarrhea virus belongs to the genus Coronaviridae of the family Coronaviridae. Virus particles are pleomorphic, tend to be spherical, and have a diameter of 95-l90nm. There is a capsule outside, and there are petal-like fibrous protrusions on the capsule. The nucleic acid type of porcine epidemic diarrhea virus is RNA type. In 1995, the sixth report of the International Committee on Taxonomy of Viruses (CTV) listed PEDV as ...

Examples

preparation example Construction

[0035] Preparation of Bacillus subtilis bacterial agent:

[0036] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;

[0037] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;

[0038] (3) Tertiary seed cultivation: Inoculate the secondary seeds with 10% inoculum amount into a 5000 ml tertiary seed shaker flask, with a medium capacity of 1000 ml, a rotary shaker at 100 rpm, and a culture temperature of 30°C. Time 24 hours;

[0039] (4) First-level seed tank cultivation: put the third-level seeds into the first-level seed tank with a total volume of 150L with 10% inoculation amount, the fermentation medium capacity is 100L, the cul...

Embodiment 1

[0066] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:

[0067] Centella asiatica powder 10 parts; turmeric powder 5 parts; cassia seed powder 8 parts; burdock seed powder 10 parts; 5 parts of bacterial agent.

[0068] The preparation method of described Chinese herbal medicine preparation is as follows:

[0069] Accurately weigh 20 parts of Centella asiatica; 10 parts of cassia seed; 5 parts of arctium; Keep the temperature at 70°C for 2 hours, add a mixture of ethanol and propanol twice the weight of the mixed material, control the temperature to 60°C, keep it for 3 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.

[0070] The mass ratio of ethanol and propanol is 1:1; the ethanol concentration is 95%; the propanol concentration is 100%;

[0071] The preparation ...

Embodiment 2

[0078] A Chinese herbal medicine extract feed additive capable of effectively preventing and treating porcine epidemic diarrhea consists of the following raw materials in parts by weight:

[0079] 15 parts of centella asiatica powder; 10 parts of turmeric powder; 15 parts of cassia seed powder; 20 parts of burdock seed powder; 10 parts of bacterial agent.

[0080] The preparation method of described Chinese herbal medicine preparation is as follows:

[0081] Accurately weigh 25 parts of Centella Asiatica; 18 parts of Semen Cassiae; 15 parts of Arctium; Keep the temperature at 90°C for 4 hours, add a mixture of ethanol and propanol 3 times the weight of the mixed material, control the temperature to 78°C, keep it for 4 hours, filter; the filtrate is concentrated in vacuum and then freeze-dried to obtain a Chinese herbal medicine preparation.

[0082] The mass ratio of described ethanol and propanol is 1:1.5, and ethanol concentration is 95%; Propanol concentration is 100%;

...