Seed selection method and breeding method of yellow-bud pimento cytoplasmic male sterile line

Abstract

The invention relates to a seed selection method and a breeding method of a yellow-bud pimento cytoplasmic male sterile line. A yellow-bud pimento cytoplasmic male sterile plant is used as a female parent and a green-bud pimento is used as a male parent to be hybridized to obtain a hybridized seed first generation F1; the F1 is self-crossed to obtain a self-crossed first generation F2; the F2 is sowed; a yellow-bud complete sterile plant AF2 is used as a female parent and a yellow-bud fertile plant BF2 is used as a male parent to be hybridized to obtain a sterile plant hybridized second generation AF3; the yellow-bud fertile plant BF2 is used as a female parent and the green-bud pimento is used as a male parent to be hybridized to obtain a sterile plant hybridized seed second generation BF3; the BF3 is self-crossed to obtain a self-crossed second generation BF4; an AF3 and a BF4 are sowed; a complete sterile plant seed second generation AF3 with a yellow bud mark and a sterile plant second generation BF4 are selected to be hybridized to obtain a sterile plant hybridized third generation AF4; the BF4 and the green-bud pimento are hybridized to obtain a sterile plant hybridized third generation BF5; the BF5 is self-crossed to obtain a self-crossed seed third generation; and the steps are repeated to obtain a yellow-bud pimento cytoplasmic male sterile line seed.

Description

technical field

[0001] The invention relates to a method for selecting and breeding a cytoplasmic male sterile line of sweet pepper, in particular to a method for selecting and breeding a cytoplasmic male sterile line of bud yellow sweet pepper, and belongs to the technical field of plant cultivation. Background technique

[0002] As far as the applicant knows, sweet peppers have obvious heterosis. At present, the ways to utilize the heterosis of sweet pepper include artificial emasculation interspecific hybridization, double-cross, three-cross and male sterile line seed production, and male sterile line seed production is divided into male sterile dual-purpose line hybrid production There are two types of seed production and cytoplasmic male sterile line. Cytoplasmic male sterility (CMS) of sweet pepper has the advantage of stable sterility, but it is necessary to remove 50% of the female parent of the male sterile line at the initial flowering stage when producing swe...

Examples

Embodiment 1

[0027] In the first spring of this example, bud yellow sweet pepper cytoplasmic male sterile strain S140-2A (self-selected as a unit) was used as the female parent, and FS-32 sweet pepper (green seedling sweet pepper) was used as the male parent for artificial assisted pollination The first generation of hybrid F1 (green leaves of F1) is obtained by crossing. The first generation of hybrid F1 is sown in the autumn of the year, and the fertile plants (or part of the fertile plants) are selected by pollen microscope for self-crossing to obtain the first generation of self-bred F2. In the spring of the second year, the first generation F2 of the inbred species was sown, the green seedlings were eliminated, and the first generation of completely sterile plants (number FS-32AF2) and the first generation of fertile plants (number FS-32AF2) with bud yellow marks were selected by visual inspection and pollen microscope inspection. 32BF2), and using the first generation of Yahuang compl...

technical field

[0001] The invention relates to a method for selecting and breeding a cytoplasmic male sterile line of sweet pepper, in particular to a method for selecting and breeding a cytoplasmic male sterile line of bud yellow sweet pepper, and belongs to the technical field of plant cultivation. Background technique

[0002] As far as the applicant knows, sweet peppers have obvious heterosis. At present, the ways to utilize the heterosis of sweet pepper include artificial emasculation interspecific hybridization, double-cross, three-cross and male sterile line seed production, and male sterile line seed production is divided into male sterile dual-purpose line hybrid production There are two types of seed production and cytoplasmic male sterile line. Cytoplasmic male sterility (CMS) of sweet pepper has the advantage of stable sterility, but it is necessary to remove 50% of the female parent of the male sterile line at the initial flowering stage when producing swe...

Examples

Embodiment 1

[0027] In the first spring of this example, bud yellow sweet pepper cytoplasmic male sterile strain S140-2A (self-selected as a unit) was used as the female parent, and FS-32 sweet pepper (green seedling sweet pepper) was used as the male parent for artificial assisted pollination The first generation of hybrid F1 (green leaves of F1) is obtained by crossing. The first generation of hybrid F1 is sown in the autumn of the year, and the fertile plants (or part of the fertile plants) are selected by pollen microscope for self-crossing to obtain the first generation of self-bred F2. In the spring of the second year, the first generation F2 of the inbred species was sown, the green seedlings were eliminated, and the first generation of completely sterile plants (number FS-32AF2) and the first generation of fertile plants (number FS-32AF2) with bud yellow marks were selected by visual inspection and pollen microscope inspection. 32BF2), and using the first generation of Yahuang compl...

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