Kanamycin, erythrocin and lincomycin three-in-one micro-hole gold-labeled test strip

Abstract

The invention discloses a kanamycin, erythrocin and lincomycin three-in-one gold-labeled micro-hole strip. A kit comprises a sample plate at least provided with one sample groove and at least one test strip; a colloidal gold-labeled object of a kanamycin specific antibody, a colloidal gold-labeled object of an erythrocin specific antibody and a colloidal gold-labeled object of a lincomycin specific antibody are attached to the bottom of each sample groove; the test strip comprises a bottom plate (1), a reaction film (2), a sample absorbent pad (3) and an absorbent pad (4), wherein the sample absorbent pad, the reaction film and the absorbent pad are sequentially attached to the bottom plate; a line T3, a line T2, a line T1 and a line C are sequentially arranged on the reaction film from beginning to tail end, and the line T3, the line T2 and the line T1 are coated with a lincomycin-carrier protein coupling, an erythrocin-carrier protein coupling and a kanamycin-carrier protein coupling respectively; the C line is coated with an antiantibody. The kit has the wide market prospect.

Description

technical field

[0001] The invention belongs to the field of food safety detection, in particular to a method for simultaneously detecting the contents of kanamycin, erythromycin and lincomycin in animal-derived foods (such as milk, etc.) Mycin three-in-one gold standard microporous test strip. Background technique

[0002] Kanamycin (Kanamycin) belongs to aminoglycoside antibiotics, has good antibacterial effect on various Gram-negative bacteria and Gram-positive bacteria, and is suitable for treating bacillary dysentery or other bacterial intestinal infections. One of the veterinary drugs commonly used in my country's animal husbandry and aquaculture, it is also often added to feed to promote the growth and development of animals. Although kanamycin has good pharmacological effects, its ototoxicity and nephrotoxicity are also relatively serious, so its residues in animal products have increasingly attracted people's attention. In order to strictly control the residues of ...

Examples

Embodiment 1

[0064] Embodiment 1, the preparation of the colloidal gold marker of conjugate, specific antibody and specific antibody

[0065] 1. Preparation of conjugates

[0066] 1. Preparation of kanamycin-carrier protein conjugates

[0067] (1) Weigh 50mg of kanamycin API, dissolve it in 4.1ml of carbonate buffer solution (pH9.5, 0.1M), and stir until completely dissolved.

[0068] (2) Weigh 70mg of BSA, dissolve in 5ml of carbonate buffer (pH9.5, 0.1M), stir at 400rpm for 10min, and fully dissolve.

[0069] (3) Add the kanamycin solution obtained in step (1) dropwise to the BSA solution obtained in step (2) and mix well, then slowly add 0.9ml of 1% (volume ratio) glutaraldehyde aqueous solution and stir at room temperature 1.5h, then add NaBH dropwise 4 solution (6mg NaBH 4 Dissolve in 1ml of pure water (newly prepared) and react at room temperature for 2 hours, then place in a dialysis bag and use PBS buffer (0.01M, pH7.2) for dialysis (dialysis process parameters: room temperatur...

Embodiment 2

[0121] Embodiment 2, the assembly of kit

[0122] figure 1 It is a schematic diagram of the structure of the test strip. figure 2 It is a schematic diagram of the use state of the kit. image 3 Schematic diagram for the interpretation of the results of the kit.

[0123] The kit for detecting kanamycin, erythromycin and lincomycin provided by the present invention includes the sample plate prepared in step 3 of Example 1 and 8 identical test strips. The kit also includes an aluminum foil bag, and the sample plate and the test strip are packaged in the aluminum foil bag. Each test strip includes a bottom plate 1 , a reaction film 2 , a sample absorbent pad 3 and a water absorbent pad 4 . The sample absorbent pad, the reaction membrane and the water absorbent pad are attached to the bottom plate in sequence, the end of the sample absorbent pad is connected to the beginning of the reaction membrane, and the end of the reaction membrane is connected to the water absorbent pad....

technical field

[0001] The invention belongs to the field of food safety detection, in particular to a method for simultaneously detecting the contents of kanamycin, erythromycin and lincomycin in animal-derived foods (such as milk, etc.) Mycin three-in-one gold standard microporous test strip. Background technique

[0002] Kanamycin (Kanamycin) belongs to aminoglycoside antibiotics, has good antibacterial effect on various Gram-negative bacteria and Gram-positive bacteria, and is suitable for treating bacillary dysentery or other bacterial intestinal infections. One of the veterinary drugs commonly used in my country's animal husbandry and aquaculture, it is also often added to feed to promote the growth and development of animals. Although kanamycin has good pharmacological effects, its ototoxicity and nephrotoxicity are also relatively serious, so its residues in animal products have increasingly attracted people's attention. In order to strictly control the residues of ...

Examples

Embodiment 1

[0064] Embodiment 1, the preparation of the colloidal gold marker of conjugate, specific antibody and specific antibody

[0065] 1. Preparation of conjugates

[0066] 1. Preparation of kanamycin-carrier protein conjugates

[0067] (1) Weigh 50mg of kanamycin API, dissolve it in 4.1ml of carbonate buffer solution (pH9.5, 0.1M), and stir until completely dissolved.

[0068] (2) Weigh 70mg of BSA, dissolve in 5ml of carbonate buffer (pH9.5, 0.1M), stir at 400rpm for 10min, and fully dissolve.

[0069] (3) Add the kanamycin solution obtained in step (1) dropwise to the BSA solution obtained in step (2) and mix well, then slowly add 0.9ml of 1% (volume ratio) glutaraldehyde aqueous solution and stir at room temperature 1.5h, then add NaBH dropwise 4 solution (6mg NaBH 4 Dissolve in 1ml of pure water (newly prepared) and react at room temperature for 2 hours, then place in a dialysis bag and use PBS buffer (0.01M, pH7.2) for dialysis (dialysis process parameters: room temperatur...

Embodiment 2

[0121] Embodiment 2, the assembly of kit

[0122] figure 1 It is a schematic diagram of the structure of the test strip. figure 2 It is a schematic diagram of the use state of the kit. image 3 Schematic diagram for the interpretation of the results of the kit.

[0123] The kit for detecting kanamycin, erythromycin and lincomycin provided by the present invention includes the sample plate prepared in step 3 of Example 1 and 8 identical test strips. The kit also includes an aluminum foil bag, and the sample plate and the test strip are packaged in the aluminum foil bag. Each test strip includes a bottom plate 1 , a reaction film 2 , a sample absorbent pad 3 and a water absorbent pad 4 . The sample absorbent pad, the reaction membrane and the water absorbent pad are attached to the bottom plate in sequence, the end of the sample absorbent pad is connected to the beginning of the reaction membrane, and the end of the reaction membrane is connected to the water absorbent pad....

Images