Application of CPT (Cryptotanshinone) in preparing medicine for preventing and treating recurrence and metastasis of tumor

A technology of cryptotanshinone, recurrence and metastasis, applied in the direction of antineoplastic drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems that the intervention effect of tumor stem cells has not been reported, and achieve the regulation of tumor-related immune responses, good recurrence and metastasis, and prevention of recurrence and metastasis Effect

CN107519186AInactive Publication Date: 2017-12-29GUANGANMEN HOSPITAL CHINA ACAD OF CHINESE MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-12-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses application of CPT (Cryptotanshinone) in preparing medicine for preventing and treating recurrence and metastasis of tumor. A CPT traditional Chinese medicine monomer is single and definite in composition. According to the application disclosed by the invention, the recurrence and metastasis of the tumor can be well prevented while the CPT traditional Chinese medicine monomer is used for killing tumor cells, the anti-tumor therapeutic effect of the CPT traditional Chinese medicine monomer is actual and reliable, and the mechanism is definite and clear. The CPT traditional Chinese medicine monomer has very good functions in aspects of inhibiting tumor cell proliferation, regulating and controlling the functions of tumor stem cells, inhibiting inflammatory responses related to the tumor, regulating immune responses related to the tumor and the like, and can be used for treating the tumor. The medicine has the function of regulating biological behaviors of the tumor stem cells so as to inhibit the recurrence and metastasis of the tumor.
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Description

technical field

[0001] The invention relates to the application of cryptotanshinone in the preparation of drugs for preventing and treating tumor recurrence and metastasis. Background technique

[0002] Currently, the existing treatment methods for malignant tumors are limited to clearing tumor cells, but there is a lack of effective drugs to prevent the recurrence and metastasis of latent malignant tumor cells in the body. The treatment of malignant tumors has always been a world problem that plagues mankind, and its morbidity and mortality have been increasing year by year. Although various countries in the world have invested a lot of resources in the field of tumor research in recent years, the momentum has not diminished. Existing tumor treatment methods are mainly surgery, radiotherapy and chemotherapy. Chemotherapy has a narrow target range and low efficiency; the toxic and side effects are too large for patients to tolerate; it can only kill tumor cells but cannot co...

Examples

Embodiment 1

[0050] Example 1 Tumor stem cell culture, purification and identification of DU145 prostate cancer cell line

[0051] (1) Tumor stem cell culture and purification of DU145 prostate cancer cell line

[0052] Through the in vitro culture of DU145 prostate cancer cell line, prostate cancer stem cell-like cells were obtained, further purified and enriched, and the morphological differences between DU145 cells and prostate cancer stem cell-like cells were compared.

[0053] 1 material

[0054] 1.1 cells

[0055] Human prostate cancer DU145 cells were purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences.

[0056] 1.2 Main reagents

[0057] MEM medium was purchased from Gibco;

[0058] Fetal bovine serum was purchased from Gibco;

[0059] Trypsin was purchased from Hyclone Company;

[0060] Penicillin streptomycin was purchased from Hyclone Company;

[0061] PBS was purchased from Hyclone;

[0062] DMEM-F12 medium was purchased from Hyclone Company; ...

Embodiment 2

[0163] Example 2 Study on the Proliferation Intervention Effect of Chinese Medicine Monomers on DU145 Cells and Prostate Cancer Stem Cells

[0164] 1 material

[0165] 1.1 cells

[0166] DU145 prostate cancer cells and prostate cancer stem cells DU145-CSC cultured and purified to the third generation under SFM conditions.

[0167] 1.2 Main reagents

[0168] Serum-supplemented medium (SSM) is: 10% fetal bovine serum + MEM medium + 1% penicillin-streptomycin double antibody;

[0169] Serum-free medium (serum-free medium, SFM) configuration: DMEM-F12 medium+10ng / ml b-FGF+20ng / mlEGF+1:50B27+5ug / ml+insulin+0.4%BSA+2%KnockOut+ 1% Pen / Strep;

[0170] Cell-counting kit-8 kit was purchased from Japan Dojin Chemical Co., Ltd.

[0171] 1.3 Drugs

[0172] Cryptotanshinone (No. 110852, specification 20mg), peiminin A (No. 110750, specification 20mg), curcumin (No. 110823, specification 20mg), curcumol (No. 100185, specification 100mg), gambogic acid number (No. 111966, specification ...

Embodiment 3

[0204] Example 3 Cryptotanshinone Intervenes in Prostate Cancer Stem Cell Specific Biological Behavior Network Regulatory Mechanism

[0205] Prostate cancer stem cells themselves are regulated by multiple genes and multiple signaling pathways, and these signaling pathways form an intercommunicating network system with JAK / STAT, PI3K / AKT, and Ras / MAPK as the core. It can be seen from Example 2 that cryptotanshinone can inhibit the biological behaviors such as cell proliferation of prostate cancer stem cells. In order to explore the network regulation mechanism of cryptotanshinone interfering with the specific biological behavior of prostate cancer stem cells, we used protein microarray technology to compare the differential expression of protein phosphorylation between DU145-CSC and CT-CSC after cryptotanshinone intervention.

[0206] 1 material

[0207] 1.1 cells

[0208] The prostate cancer stem cell-like cell microspheres (TumorSpheres) that were purified to the third gene...