Method for extracting fucoidan from kelp by using ultrasonic waves

Abstract

The invention relates to the technical field of deep processing of marine resources, and particularly discloses a method for extracting fucoidan from kelp by using ultrasonic waves. According to the method, kelp is used as a raw material; and the method comprises: washing kelp, drying, and crushing; carrying out degreasing treatment on the crushed kelp; carrying out centrifugation on the degreasedkelp, collecting the precipitate, and drying to obtain kelp powder; mixing the kelp powder and water, adding composite enzyme comprising cellulase, hemicellulase, protease and pectinase, and carryingout enzymolysis; ultrasonically extracting the kelp powder obtained after the enzymolysis; removing the supernatant from the ultrasonically treated solution, concentrating, and adding ethanol; and carrying centrifugation on the concentrated fucoidan, and washing the separated precipitate by using ethanol to obtain the crude fucoidan. According to the present invention, the method has advantages of simple operation, short extraction time and low energy consumption, wherein the extraction work is performed at the low temperature, such that the damage of the high temperature on the extracted component can be avoided while the precipitation of the pigment during the extraction can be reduced.

Description

(1) Technical field

[0001] The invention relates to the technical field of deep processing of marine resources, in particular to a method for extracting fucoidan from kelp by using ultrasonic waves. (2) Background technology

[0002] Fucoidan is an intercellular polysaccharide present in all brown algae. It was first extracted from the brown alga Laminaria palmatum by Kylin in 1913. Fucoidan, also known as fucoidan sulfate, is a heteropolysaccharide containing galactose, xylose, mannose and uronic acid in addition to fucose and sulfate. Recent studies have shown that fucoidan has a variety of physiological activities, such as antibacterial, antiviral (including anti-HIV), antitumor, immune regulation, anticoagulant, and hypolipidemic functions. Due to the rich resources of brown algae, the research on fucoidan, the main active ingredient of brown algae, has attracted more and more attention from scholars at home and abroad. In order to study the clear structure and exact f...

Examples

Embodiment

[0025] Embodiment: a method for extracting fucoidan from kelp by ultrasonic waves, comprising the following steps:

[0026] (1) Cleaning Rinse the fresh kelp without mildew and browning with pure water;

[0027] (2) Drying Dry the cleaned kelp at a temperature of 40°C;

[0028] (3) Pulverize the dried kelp to 40 mesh with a pulverizer;

[0029] (4) Degreasing The pulverized kelp powder is degreased and refluxed twice with 80% ethanol, each time for 4 hours, and the ratio of solid to liquid is 1:20 (g / ml);

[0030] (5) Centrifugation and drying Centrifuge the degreased material in a centrifuge to collect the precipitate and dry it. The centrifuge speed is 3000 rpm, the centrifugation time is 10 minutes, and the drying temperature is 40°C;

[0031] (6) Enzymolysis Mix the dried kelp powder and water in a certain proportion and add cellulase, protease and pectinase compound enzyme for enzymolysis; after the enzymolysis time is over, quickly heat up to 90°C to inactivate the enz...

(1) Technical field

[0001] The invention relates to the technical field of deep processing of marine resources, in particular to a method for extracting fucoidan from kelp by using ultrasonic waves. (2) Background technology

[0002] Fucoidan is an intercellular polysaccharide present in all brown algae. It was first extracted from the brown alga Laminaria palmatum by Kylin in 1913. Fucoidan, also known as fucoidan sulfate, is a heteropolysaccharide containing galactose, xylose, mannose and uronic acid in addition to fucose and sulfate. Recent studies have shown that fucoidan has a variety of physiological activities, such as antibacterial, antiviral (including anti-HIV), antitumor, immune regulation, anticoagulant, and hypolipidemic functions. Due to the rich resources of brown algae, the research on fucoidan, the main active ingredient of brown algae, has attracted more and more attention from scholars at home and abroad. In order to study the clear structure and exact f...

Examples

Embodiment

[0025] Embodiment: a method for extracting fucoidan from kelp by ultrasonic waves, comprising the following steps:

[0026] (1) Cleaning Rinse the fresh kelp without mildew and browning with pure water;

[0027] (2) Drying Dry the cleaned kelp at a temperature of 40°C;

[0028] (3) Pulverize the dried kelp to 40 mesh with a pulverizer;

[0029] (4) Degreasing The pulverized kelp powder is degreased and refluxed twice with 80% ethanol, each time for 4 hours, and the ratio of solid to liquid is 1:20 (g / ml);

[0030] (5) Centrifugation and drying Centrifuge the degreased material in a centrifuge to collect the precipitate and dry it. The centrifuge speed is 3000 rpm, the centrifugation time is 10 minutes, and the drying temperature is 40°C;

[0031] (6) Enzymolysis Mix the dried kelp powder and water in a certain proportion and add cellulase, protease and pectinase compound enzyme for enzymolysis; after the enzymolysis time is over, quickly heat up to 90°C to inactivate the enz...

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