Small fully automatic chemiluminescence immunoassay analyzer and method of use

Abstract

A small fully automatic chemiluminescence immunoassay analyzer and a method of using the same. The product comprises: a bottom top plate (26), a power supply (9) fixed on the bottom top plate (26), the power supply (9) connected to a main control board (8), the main control board (8) connected to a detection module (10) and an incubation module (11), the incubation module (11) connected to the bottom top plate (26), the incubation module (11) connected to a tip rack (12), a frame top plate (27) arranged on the main control board (8), and the frame top plate (27) connected to a sample loading module (13). The present invention is used for a chemiluminescence immunoassay analyzer.

Description

Technical field:

[0002] The invention relates to a small fully automatic chemiluminescence immunoassay analyzer and a use method thereof. Background technology:

[0004] Chemiluminescent immunoassay is a technique that combines highly sensitive chemiluminescent assay technology with highly specific immune reactions, and is used for the detection and analysis of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, and drugs. It is the latest immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay, and time-resolved fluorescence immunoassay. As the current mainstream immunoassay technology, in actual use, the instrument size is relatively large, the structure is complex, and the product price is high. At present, it can only be used in professional testing departments of hospitals. Therefore, it cannot be used in primary hospitals or emergency departments that require emergency treatment.

[0005] As my country's medical system continues to improve and the tiered diagnosis and treatment service capabilities are comprehensively enhanced, the demand for medical equipment in primary hospitals will gradually increase. Therefore, the development of a chemiluminescence immunoassay system for primary hospitals and emergency departments is of great significance. Summary of the invention:

[0007] The purpose of the present invention is to provide a small-sized, compact structure, no liquid pipeline, and a small-sized fully automatic chemiluminescence immunoassay analyzer with reduced maintenance probability, and a method for using the same.

[0008] The above purpose is achieved through the following technical solutions:

[0009] A small fully automatic chemiluminescence immunoassay analyzer comprises: a bottom top plate (26), a power supply (9) fixed on the bottom top plate (26), the power supply (9) connected to a main control board (8), the main control board (8) connected to a detection module (10) and an incubation module (11), the incubation module (11) connected to the bottom top plate (26), the incubation module (11) connected to a tip rack (12), a frame top plate (27) arranged above the main control board (8), and the frame top plate (27) connected to a sample loading module (13).

[0010] The small fully automatic chemiluminescence immunoassay analyzer, the incubation module (11) comprises a heating base plate (28), the heating base plate (28) is connected to a heating plate (15), the heating base plate (28) is connected to an incubation tank (14), and the incubation tank (14) is connected to a magnet frame (16).

[0011] The small fully automatic chemiluminescence immunoassay analyzer has a reagent kit inserted into the incubation tank (14), the reagent kit comprises a box body (29), the box body (29) is connected to an upper cover (30), the upper cover (30) has a group of fixed openings (31), and the fixed openings (31) are sequentially connected to a reaction cup (1), a substrate cup (2), a magnetic particle cup (3), a washing liquid cup (4), a diluent cup (5), a reagent cup (6), and a sample cup (7).

[0012] The small fully automatic chemiluminescence immunoassay analyzer, the sample loading module (13) comprises a frame (32), the frame (32) is connected to the sample loading module Z linear motor (20) and the sample loading module Y axis motor (21), the sample loading module Z linear motor (20) is connected to the syringe (18), the syringe (18) is connected to the syringe linear motor (17), and the syringe (18) is connected to the hole opener (19).

[0013] The small fully automatic chemiluminescence immunoassay analyzer, the detection module (10) comprises a detector (22), the detector (22) is connected to a probe sealing structure (24), the detector (22) is connected to a probe (23), and the detector (22) is connected to a detection module Z-axis linear motor (25).

[0014] A method for using a small fully automatic chemiluminescence immunoassay analyzer comprises the following steps: placing a serum or whole blood sample in a sample cup (7) of a reagent box with a dropper, inserting the reagent box into an incubation tank (14) of an incubation module, placing a pipette tip on a pipette tip rack (12), moving a Y-axis motor (21) of a sample loading module, moving a syringe (18) above the pipette tip, moving a Z-axis linear motor (20) of a sample loading module, moving the syringe (18) downward by a certain distance, inserting a pipette tip adapter under the syringe (18) into the pipette tip, and tightly fitting the pipette tip adapter to form a sealing structure, sucking liquid through the pipette tip, moving the Z-axis linear motor (20) of the sample loading module, moving the syringe (18) upward and returning to the origin, and the pipette tip is also lifted upward, thereby completing the action of loading the pipette tip.

[0015] The method for using the small fully automatic chemiluminescence immunoassay analyzer is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the sample cup (7) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the sample, the syringe linear motor (17) moves, and a certain amount of sample is sucked; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward;

[0016] The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the reagent cup (6) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the reagent. The syringe linear motor (17) moves to absorb a certain amount of reagent. The Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0017] The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the diluent cup (5) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the diluent, the syringe linear motor (17) moves, and the sucked sample and reagent are injected into the diluent, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the diluent, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, the syringe linear motor (17) moves to suck a certain amount of diluent; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward;

[0018] The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the magnetic particle cup (3) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the magnetic particle liquid, the syringe linear motor (17) moves, and the sucked diluent is injected into the magnetic particle liquid, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the magnetic bead liquid, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, it needs to be incubated for a certain time;

[0019] After a certain period of incubation, the Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the pipette tip drops below the liquid surface of the magnetic particle liquid. The syringe linear motor (17) moves to absorb all the magnetic particle liquid. The magnetic particles in the liquid in the pipette tip are attracted by the magnet in the magnet frame (16). The magnetic particles will gather on the inner wall of the pipette tip close to the magnet. After the magnetic particles are completely adsorbed on the inner wall of the pipette tip, the syringe linear motor (17) moves to inject the reaction liquid in the pipette tip into the magnetic particle cup (3). The magnetic particles still stay on the inner wall of the pipette tip. The Z-axis linear motor (20) of the sample loading module moves, and the pipette tip moves upward; the separation of the magnetic beads and the reaction liquid is completed;

[0020] The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the washing liquid cup (4) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the washing liquid. The syringe linear motor (17) reciprocates to complete the cleaning of the magnetic beads by the washing liquid. The magnetic particles remain on the inner wall of the suction head due to the attraction of the magnet. The above actions are repeated to complete the cleaning work in other washing liquid cups.

[0021] The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the substrate cup (2) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the substrate liquid. The syringe linear motor (17) moves to absorb a certain amount of substrate liquid. The Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0022] The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the reaction cup (1) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, the suction head is lowered into the reaction cup, the syringe linear motor (17) moves to inject the sucked substrate solution into the reaction cup, the syringe linear motor (17) reciprocates to complete the mixing action of the magnetic beads and the substrate solution, the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward;

[0023] The Y-axis motor (21) of the sample loading module moves, the syringe (18) and the pipette tip leave the top of the reaction cup (1), the Z-axis linear motor (25) of the detection module moves, the detector (22) descends, and when the probe (23) contacts the top of the reaction cup (1), the light shield in the probe sealing structure (24) opens, and the light emitted by the substrate liquid in the reaction cup (1) passes through the probe (23) and is received by the photomultiplier tube in the detector (22), and is converted into an electrical signal. After being processed by a computer, the substance concentration of the sample being tested is obtained.

[0024] Beneficial effects:

[0025] 1. The present invention has the characteristics of small size, compact structure, no liquid pipeline, eliminating a lot of maintenance work, low failure rate, etc., which is very beneficial for grassroots testing agencies to carry out testing work.

[0026] The present invention adopts a reagent kit structure, which can integrate the substances involved in the reaction into one box, and can be loaded with sample cups, reagent cups, washing liquid cups, diluent cups, substrate cups, reaction cups, etc., with good loading effect.

[0027] The present invention adopts a sample adding structure, which is composed of multiple syringes, forming multiple sample adding channels and capable of moving in three directions of X, Y and Z. The syringes are driven by linear motors and can perform tasks such as sample adding, mixing and cleaning.

[0028] The present invention adopts an incubation structure, which has multiple slots and can be used to insert multiple reagent kits at the same time. The reagent kits are tightly fitted with the slots, so that the temperature of the slots can be quickly transmitted to the reagent kits. The slots are heated by a heating plate, and the slots are provided with temperature sensors to monitor the temperature of the slots in real time. The heating temperature of the slots can be accurately controlled through circuit control. In addition, a magnet is installed on the top of the slots. When cleaning and separating magnetic particles, the suction head is in close contact with the surface of the magnet. Under the action of the magnetic field, the magnetic particles are attracted to the inner wall of the suction head, and the syringe is driven to discharge the reaction liquid out of the suction head, thereby achieving the purpose of separating the magnetic particles from the reaction liquid.

[0029] The present invention adopts a detection structure, which has a shading plate at the detection window end of the detector. It is usually in a closed state to prevent the damage of natural light from the outside to the photomultiplier tube. During detection, the detector is driven by a Z-axis linear motor to move downward, and the sealing ring on the probe is tightly connected to the reaction cup to prevent external light from entering. The sealing structure of the probe is blocked by a limiting structure, and the shading plate is removed through a connecting rod structure to realize the detection function. Description of the drawings:

[0031] Attached Figure 1 It is a structural diagram of this product.

[0032] Attached Figure 2 Yes Figure 1 Schematic diagram of the structure of the incubation module.

[0033] Attached Figure 3 Yes Figure 1 Schematic diagram of the structure of the kit.

[0034] Attached Figure 4 Yes Figure 1 Schematic diagram of the structure of the sample loading module.

[0035] Attached Figure 5 Yes Figure 1 Schematic diagram of the incubation module structure. Specific implementation method:

[0037] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention.

[0038] Embodiment 1:

[0039] A small fully automatic chemiluminescence immunoassay analyzer comprises: a bottom top plate (26), a power supply (9) fixed on the bottom top plate (26), the power supply (9) connected to a main control board (8), the main control board (8) connected to a detection module (10) and an incubation module (11), the incubation module (11) connected to the bottom top plate (26), the incubation module (11) connected to a tip rack (12), a frame top plate (27) arranged above the main control board (8), and the frame top plate (27) connected to a sample loading module (13).

[0040] Embodiment 2:

[0041] In the small fully automatic chemiluminescence immunoassay analyzer described in Example 1, the incubation module (11) includes a heating base plate (28), the heating base plate (28) is connected to the heating plate (15), the heating base plate (28) is connected to the incubation tank (14), and the incubation tank (14) is connected to the magnet frame (16).

[0042] Embodiment 3:

[0043] In the small fully automatic chemiluminescence immunoassay analyzer described in Example 2, a reagent kit is inserted into the incubation tank (14), and the reagent kit includes a box body (29), the box body (29) is connected to an upper cover (30), and the upper cover (30) is provided with a group of fixed ports (31), and the fixed ports (31) are sequentially connected to a reaction cup (1), a substrate cup (2), a magnetic particle cup (3), a washing liquid cup (4), a diluent cup (5), a reagent cup (6), and a sample cup (7).

[0044] Embodiment 4:

[0045] In the small fully automatic chemiluminescence immunoassay analyzer described in Example 1, the sample loading module (13) comprises a frame (32), the frame (32) is connected to the sample loading module Z linear motor (20) and the sample loading module Y axis motor (21), the sample loading module Z linear motor (20) is connected to the syringe (18), the syringe (18) is connected to the syringe linear motor (17), and the syringe (18) is connected to the hole opener (19).

[0046] Embodiment 5:

[0047] In the small fully automatic chemiluminescence immunoassay analyzer described in Example 1, the detection module (10) includes a detector (22), the detector (22) is connected to the probe sealing structure (24), the detector (22) is connected to the probe (23), and the detector (22) is connected to the detection module Z-axis linear motor (25).

[0048] Embodiment 6:

[0049] A method for using a small fully automatic chemiluminescence immunoassay analyzer comprises the following steps: placing a serum or whole blood sample in a sample cup (7) of a reagent box with a dropper, inserting the reagent box into an incubation tank (14) of an incubation module, placing a pipette tip on a pipette tip rack (12), moving a Y-axis motor (21) of a sample loading module, moving a syringe (18) above the pipette tip, moving a Z-axis linear motor (20) of a sample loading module, moving the syringe (18) downward by a certain distance, inserting a pipette tip adapter under the syringe (18) into the pipette tip, and tightly fitting the pipette tip adapter to form a sealing structure, so that liquid can be sucked through the pipette tip, moving the Z-axis linear motor (20) of the sample loading module, moving the syringe (18) upward, returning to the origin, and lifting the pipette tip upward, thereby completing the action of loading the pipette tip.

[0050] Embodiment 7:

[0051] The method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6 is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the sample cup (7) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the sample, and the syringe linear motor (17) moves to absorb a certain amount of sample; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0052] Embodiment 8:

[0053] In the method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6, the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the reagent cup (6) of the reagent kit, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid level of the reagent, the syringe linear motor (17) moves, and a certain amount of reagent is sucked; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0054] Embodiment 9:

[0055] In the method for using the small fully automatic chemiluminescence immunoassay instrument described in Example 6, the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the diluent cup (5) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the diluent, the syringe linear motor (17) moves, and the sucked sample and reagent are injected into the diluent, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the diluent, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, the syringe linear motor (17) moves to suck a certain amount of diluent; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0056] Embodiment 10:

[0057] The method for using the small fully automatic chemiluminescence immunoassay instrument described in Example 6 is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the magnetic particle cup (3) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the magnetic particle liquid, the syringe linear motor (17) moves, and the sucked diluent is injected into the magnetic particle liquid, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the magnetic bead liquid, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, it needs to be incubated for a certain period of time;

[0058] After a certain period of incubation, the Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the pipette tip drops below the liquid surface of the magnetic particle liquid. The syringe linear motor (17) moves to absorb all the magnetic particle liquid. The magnetic particles in the liquid in the pipette tip are attracted by the magnet in the magnet frame (16). The magnetic particles will gather on the inner wall of the pipette tip close to the magnet. After the magnetic particles are completely adsorbed on the inner wall of the pipette tip, the syringe linear motor (17) moves to inject the reaction liquid in the pipette tip into the magnetic particle cup (3). The magnetic particles still remain on the inner wall of the pipette tip. The Z-axis linear motor (20) of the sample loading module moves, and the pipette tip moves upward; the separation of the magnetic beads and the reaction liquid is completed.

[0059] Embodiment 11:

[0060] The method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6 is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the washing liquid cup (4) of the reagent kit, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the pipette tip drops below the liquid surface of the washing liquid, and the syringe linear motor (17) reciprocates to complete the cleaning of the magnetic beads by the washing liquid. The magnetic particles remain on the inner wall of the pipette tip due to the attraction of the magnet, and the above actions are repeated to complete the cleaning work in other washing liquid cups.

[0061] Embodiment 12:

[0062] The method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6 is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the substrate cup (2) of the reagent kit, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the substrate liquid, the syringe linear motor (17) moves, and a certain amount of substrate liquid is sucked; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0063] Embodiment 13:

[0064] The method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6 is as follows: the Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the reaction cup (1) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, the suction head is lowered into the reaction cup, the syringe linear motor (17) moves to inject the sucked substrate solution into the reaction cup, the syringe linear motor (17) reciprocates to complete the mixing of the magnetic beads and the substrate solution, the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward.

[0065] Embodiment 14:

[0066] In the method for using the small fully automatic chemiluminescence immunoassay analyzer described in Example 6, the Y-axis motor (21) of the sample loading module moves, the syringe (18) and the pipette tip leave the top of the reaction cup (1), the Z-axis linear motor (25) of the detection module moves, and the detector (22) descends. When the probe (23) contacts the top of the reaction cup (1), the light shield in the probe sealing structure (24) opens, and the light emitted by the substrate solution in the reaction cup (1) passes through the probe (23) and is received by the photomultiplier tube in the detector (22), and is converted into an electrical signal. The signal is processed by a computer to obtain the substance concentration of the sample being tested.

[0067] Embodiment 15:

[0068] The small fully automatic chemiluminescence immunoassay analyzer described in the above embodiment provides a test kit structure, which is characterized in that the substances involved in the reaction are integrated into a box, including a sample cup, a reagent cup, a washing liquid cup, a diluent cup, a substrate cup, a reaction cup, etc.

[0069] Provided is a sample adding structure, which is characterized by being composed of a plurality of syringes, forming a plurality of sample adding channels, and being able to realize movement in three directions of X, Y and Z. The syringes are driven by linear motors to realize tasks such as sample adding, mixing and cleaning.

[0070] Provided is an incubation structure, which is characterized by having multiple slots, into which multiple reagent kits can be inserted at the same time, and the reagent kits are tightly fitted with the slots, so that the temperature of the slots can be quickly transferred to the reagent kits, and the slots are heated by a heating plate, and the slots are provided with temperature sensors, which monitor the temperature of the slots in real time, and the heating temperature of the slots can be accurately controlled by circuit control. In addition, a magnet is installed on the top of the slots, and when the magnetic particles are cleaned and separated, the suction head is closely attached to the surface of the magnet, and the magnetic particles are attracted to the inner wall of the suction head under the action of the magnetic field, and the syringe is driven to discharge the reaction liquid out of the suction head, so as to achieve the purpose of separating the magnetic particles from the reaction liquid.

[0071] A detection structure is provided. The structure is characterized in that a shading plate is provided at the detection window end of the detector. The shading plate is usually in a closed state to prevent the damage of the photomultiplier tube by natural light from the outside. During detection, the detector is driven by a Z-axis linear motor to move downward. The sealing ring on the probe is tightly connected with the reaction cup to prevent the outside light from entering. The sealing structure of the probe is blocked by a limiting structure. The shading plate is removed through a connecting rod structure to realize the detection function.

Claims

1. A small fully automatic chemiluminescence immunoassay analyzer, comprising: a bottom plate (26), characterized in that : A power supply (9) is fixed on the bottom top plate (26), the power supply (9) is connected to the main control board (8), the main control board (8) is connected to the detection module (10) and the incubation module (11), the incubation module (11) is connected to the bottom top plate (26), the incubation module (11) is connected to the tip rack (12), a rack top plate (27) is arranged on the top of the main control board (8), the rack top plate (27) is connected to the sample loading module (13); the incubation module (11) includes a heating bottom plate (28), the heating bottom plate (28) is connected to the heating plate (15), the heating bottom plate (28) is connected to the incubation The incubation tank (14) is connected to the magnet frame (16); a reagent kit is inserted into the incubation tank (14), the reagent kit comprises a box body (29), the box body (29) is connected to the upper cover (30), the upper cover (30) is provided with a group of fixing ports (31), the fixing ports (31) are sequentially connected to the reaction cup (1), the substrate cup (2), the magnetic particle cup (3), the washing liquid cup (4), the diluent cup (5), the reagent cup (6), and the sample cup (7); the loading module (13) comprises a rack (32), the rack (32) is connected to the loading module Z linear motor (20) and the loading module Y axis motor (2 1), the Z linear motor (20) of the sample loading module is connected to the syringe (18), the syringe (18) is connected to the syringe linear motor (17), and the syringe (18) is connected to the hole opener (19); the detection module (10) includes a detector (22), the detector (22) is connected to the probe sealing structure (24), the detector (22) is connected to the probe (23), and the detector (22) is connected to the detection module Z axis linear motor (25); multiple reagent kits can be inserted at the same time, the reagent kits and the slot body are tightly fitted, and the temperature of the slot body can be quickly transferred to the reagent kit, the slot body is heated by the heating plate, and the slot The body is equipped with a temperature sensor to monitor the temperature of the tank in real time. Through circuit control, the heating temperature of the tank can be accurately controlled. In addition, a magnet is installed on the top of the tank. When cleaning and separating magnetic particles, the suction head is close to the surface of the magnet. Under the action of the magnetic field, the magnetic particles will be attracted to the inner wall of the suction head, driving the syringe to discharge the reaction liquid from the suction head, so as to achieve the purpose of separating the magnetic particles from the reaction liquid. There is a light shielding plate at the detection window end of the detector, which is usually in a closed state to prevent the damage of the photomultiplier tube by natural light from the outside. During detection, the detector is driven by the Z-axis linear motor to move downward, and the sealing ring on the probe is closely connected with the reaction cup to prevent the outside light from entering.A method for using a small fully automatic chemiluminescence immunoassay analyzer comprises placing a serum or whole blood sample in a sample cup (7) of a reagent box using a dropper, inserting the reagent box into an incubation tank (14) of an incubation module, placing a pipette tip on a pipette tip rack (12), moving a Y-axis motor (21) of a sample loading module, moving a syringe (18) above the pipette tip, moving a Z linear motor (20) of a sample loading module, moving the syringe (18) downward by a certain distance, inserting a pipette tip adapter under the syringe (18) into the pipette tip, and tightly fitting the pipette tip adapter to form a sealing structure. The head absorbs the liquid, the Z-axis linear motor (20) of the loading module moves, the syringe (18) moves upward and returns to the origin, and the suction head is also lifted up, completing the action of loading the suction head; the Y-axis motor (21) of the loading module moves, the syringe (18) moves to the top of the sample cup (7) of the reagent box, the Z-axis linear motor (20) of the loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the sample, and the syringe linear motor (17) moves to absorb a certain amount of sample; the Z-axis linear motor (20) of the loading module moves, and the suction head moves upward; The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the reagent cup (6) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the reagent. The syringe linear motor (17) moves to absorb a certain amount of reagent. The Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward. The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the diluent cup (5) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the diluent, the syringe linear motor (17) moves, and the sucked sample and reagent are injected into the diluent, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the diluent, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, the syringe linear motor (17) moves to suck a certain amount of diluent; the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward; The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the magnetic particle cup (3) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, so that the tip of the suction head drops above the liquid surface of the magnetic particle liquid, the syringe linear motor (17) moves, and the sucked diluent is injected into the magnetic particle liquid, the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the magnetic bead liquid, and the syringe linear motor (17) reciprocates to complete the action of mixing the liquid; after the liquid is mixed, it needs to be incubated for a certain time; After a certain period of incubation, the Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the pipette tip drops below the liquid surface of the magnetic particle liquid. The syringe linear motor (17) moves to absorb all the magnetic particle liquid. The magnetic particles in the liquid in the pipette tip are attracted by the magnet in the magnet frame (16). The magnetic particles will gather on the inner wall of the pipette tip close to the magnet. After the magnetic particles are completely adsorbed on the inner wall of the pipette tip, the syringe linear motor (17) moves to inject the reaction liquid in the pipette tip into the magnetic particle cup (3). The magnetic particles still stay on the inner wall of the pipette tip. The Z-axis linear motor (20) of the sample loading module moves, and the pipette tip moves upward; the separation of the magnetic beads and the reaction liquid is completed; The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the washing liquid cup (4) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the washing liquid. The syringe linear motor (17) reciprocates to complete the cleaning of the magnetic beads by the washing liquid. The magnetic particles remain on the inner wall of the suction head due to the attraction of the magnet. The above actions are repeated to complete the cleaning work in other washing liquid cups. The Y-axis motor (21) of the sample loading module moves, and the syringe (18) moves to the top of the substrate cup (2) of the reagent box. The Z-axis linear motor (20) of the sample loading module moves, and the syringe (18) moves downward, so that the tip of the suction head drops below the liquid surface of the substrate liquid. The syringe linear motor (17) moves to absorb a certain amount of substrate liquid. The Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward. The Y-axis motor (21) of the sample loading module moves, the syringe (18) moves to the top of the reaction cup (1) of the reagent box, the Z-axis linear motor (20) of the sample loading module moves, the syringe (18) moves downward, the suction head is lowered into the reaction cup, the syringe linear motor (17) moves to inject the sucked substrate solution into the reaction cup, the syringe linear motor (17) reciprocates to complete the mixing action of the magnetic beads and the substrate solution, the Z-axis linear motor (20) of the sample loading module moves, and the suction head moves upward; The Y-axis motor (21) of the sample loading module moves, the syringe (18) and the pipette tip leave the top of the reaction cup (1), the Z-axis linear motor (25) of the detection module moves, the detector (22) descends, and when the probe (23) contacts the top of the reaction cup (1), the light shield in the probe sealing structure (24) opens, and the light emitted by the substrate liquid in the reaction cup (1) passes through the probe (23) and is received by the photomultiplier tube in the detector (22), and is converted into an electrical signal. After being processed by a computer, the substance concentration of the sample being tested is obtained.

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