Magnetic separation cleaning device and working method of magnetic separation cleaning device

Abstract

The present invention provides a magnetic separation cleaning device and a working method thereof, wherein the magnetic separation cleaning device comprises an incubation mechanism, a fixing seat, a plurality of magnetic separation units, a liquid suction mechanism and a liquid injection mechanism; the incubation mechanism is arranged on one side of the fixing seat, and the incubation mechanism has a plurality of channels, each channel is used to accommodate a reagent strip, the reagent strip has a reaction chamber, and the reaction chamber is used to accommodate a reaction solution; a plurality of magnetic separation units are arranged on the fixing seat at intervals and each magnetic separation unit corresponds to a channel, each magnetic separation unit works independently and shares a liquid suction mechanism and a liquid injection mechanism, and each magnetic separation unit, liquid suction mechanism, and liquid injection mechanism are used to perform magnetic bead separation, waste liquid suction, and liquid injection cleaning operations on the reaction solution in the reaction chamber in the corresponding channel in sequence when the reaction solution in the reaction chamber in the corresponding channel is subjected to magnetic separation cleaning. The magnetic separation cleaning device of the present invention has a simple structure, can save installation space, and is conducive to the miniaturization of analytical instruments.

Description

Technical Field

[0001] The invention relates to the field of medical detection, and in particular to a magnetic separation cleaning device and a working method of the magnetic separation cleaning device. Background Art

[0002] In chemiluminescent immunoassay analyzers, magnetic beads are often used to collect the test substances in the test samples, and then magnetic separation is performed to purify the test substances. During the magnetic separation process, it is necessary to inject cleaning liquid into the reaction cup, and the impurities and interference items on the magnetic beads are fully removed by mixing and stirring. The magnetic beads that have reacted completely are adsorbed in the magnetic field, and the unbound substances are extracted along with the waste liquid to achieve magnetic separation. In the existing magnetic separation cleaning device, the reaction container needs to pass through the magnet in turn to complete the magnetic adsorption and waste liquid suction operations, and then pass through the position without the magnet to complete the cleaning, which makes the entire magnetic separation cleaning device complex in structure and occupies a large space. Summary of the invention

[0003] The present invention provides a magnetic separation cleaning device and a working method of the magnetic separation cleaning device. The magnetic separation cleaning device has a simple structure, can save installation space, and is conducive to the miniaturization of analytical instruments.

[0004] The present invention provides a magnetic separation and cleaning device, which comprises an incubation mechanism, a fixing seat, a plurality of magnetic separation units, a liquid suction mechanism and a liquid injection mechanism; the incubation mechanism is arranged on one side of the fixing seat, and the incubation mechanism has a plurality of channels, each of the channels is used to accommodate a reagent strip, the reagent strip has a reaction chamber, and the reaction chamber is used to accommodate a reaction liquid; a plurality of the magnetic separation units are arranged on the fixing seat at intervals and each of the magnetic separation units corresponds to one of the channels, each of the magnetic separation units works independently and shares one liquid suction mechanism and one liquid injection mechanism, and each of the magnetic separation units, the liquid suction mechanism and the liquid injection mechanism is used to perform magnetic bead separation, waste liquid suction and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the channel corresponding to the magnetic separation unit in sequence when performing magnetic separation and cleaning on the reaction liquid in the reaction chamber in the channel corresponding to the magnetic separation unit.

[0005] Each magnetic separation unit includes a driving component and a magnet, wherein the driving component is used to drive the magnet to move in a direction away from or close to a reaction chamber in a corresponding channel, wherein when the magnet is in a first position close to the reaction chamber in the corresponding channel, magnetic bead separation operations and waste liquid suction operations can be performed under the action of the magnet, and when the magnet is in a second position away from the reaction chamber in the corresponding channel, liquid injection and cleaning operations can be performed.

[0006] Wherein, the fixing seat comprises a first limiting plate, a second limiting plate and a plurality of sliding rods, the first limiting plate and the second limiting plate are arranged opposite to each other, a plurality of the sliding rods are arranged between the first limiting plate and the second limiting plate at intervals, the opposite ends of each of the sliding rods are respectively connected to the first limiting plate and the second limiting plate, and the plurality of sliding rods are arranged one by one correspondingly to the plurality of the magnetic separation units;

[0007] The magnet of each magnetic separation unit is slidably disposed on a corresponding sliding rod and is movably connected to the driving assembly, the incubation mechanism is located on a side of the second limiting plate away from the first limiting plate, and the driving assembly is used to drive the magnet to move along the sliding rod in a direction close to the incubation mechanism and move to a first position close to a reaction chamber in a corresponding channel, or the driving assembly is used to drive the magnet to move along the sliding rod in a direction away from the incubation mechanism and move to a second position away from a reaction chamber in a corresponding channel.

[0008] In which, the magnet has a first abutting end, and the incubation mechanism has a second abutting end, and the second abutting end is formed by a recessed side wall of the incubation mechanism close to the second limiting plate. When the magnet moves to the first position, the first abutting end abuts against the second abutting end, and the magnetic beads are adsorbed to the cavity wall of the reaction chamber close to the magnet by the magnetic attraction force of the magnet, and the magnetic separation unit completes the operation of adsorbing the magnetic beads in the reaction liquid in the reaction chamber in the corresponding channel.

[0009] Among them, the driving component includes an electrically connected driving motor and a driving screw, the driving motor is located on the side of the first limit plate away from the second limit plate, the driving screw is arranged between the first limit plate and the second limit plate, and the opposite ends of the driving screw are respectively connected to the first limit plate and the second limit plate, the magnet is located on the driving screw, and the driving screw is used to drive the magnet to move linearly along the driving screw under the drive of the driving motor.

[0010] Among them, the magnetic separation and cleaning device also includes a first zero position detection plate, a first zero position sensor and a controller. The first zero position detection plate is arranged at the bottom of the second limit plate, and the first zero position sensor is arranged on the first zero position detection plate. When the magnet moves to the first position, the first zero position sensor is triggered to generate a first trigger signal. When the controller receives the first trigger signal, it determines that the magnet is located at the first position and controls the magnetic bead separation operation and the waste liquid suction operation.

[0011] In which, the magnetic separation cleaning device also includes a second zero position detection plate and a second zero position sensor. The second zero position detection plate is arranged on the top of the first limit plate, and the second zero position sensor is arranged on the second zero position detection plate. When the magnet is reset to the second position, the second zero position sensor is triggered to generate a second trigger signal. When the controller receives the second trigger signal, it determines that the magnet is located at the second position and controls the subsequent liquid injection cleaning operation.

[0012] Before the magnetic separation and cleaning device performs the operation of magnetic bead adsorption through the magnetic separation unit, the liquid aspiration mechanism is also used to aspirate reagents and samples and inject the reagents and samples into the reaction chamber of the reagent strip to form the reaction solution.

[0013] Wherein, the liquid injection and cleaning operation is repeated multiple times through the liquid injection mechanism.

[0014] The present invention also provides a working method of a magnetic separation cleaning device, the magnetic separation cleaning device comprises an incubation mechanism, a plurality of magnetic separation units, a liquid suction mechanism and a liquid injection mechanism, the incubation mechanism has a plurality of channels, each of the channels is used to accommodate a reagent strip, the reagent strip has a reaction chamber, the reaction chamber is used to accommodate a reaction liquid, each of the magnetic separation units corresponds to one of the channels, the magnetic separation units work independently and share one liquid suction mechanism and one liquid injection mechanism, the magnetic separation unit is used to magnetically adsorb the reaction liquid and terminate the magnetic adsorption, the working method of the magnetic separation cleaning device comprises:

[0015] When the magnetic separation unit is controlled to generate a magnetic field for magnetic adsorption of the reaction liquid in the reaction chamber in the corresponding channel, the liquid suction mechanism is moved to absorb the waste liquid after the reaction in the reaction chamber is completed;

[0016] Controlling the magnetic separation unit to stop generating a magnetic field for the reaction liquid in the reaction chamber in the corresponding channel and stop magnetic adsorption;

[0017] Introducing cleaning fluid into the liquid injection mechanism;

[0018] The liquid injection mechanism is moved to inject the cleaning liquid in the liquid injection mechanism into the reaction chamber.

[0019] Each magnetic separation unit includes a driving component and a magnet, and controlling the magnetic separation unit to generate a magnetic field for magnetic adsorption of the reaction liquid in the reaction chamber in the corresponding channel includes:

[0020] Controlling the driving assembly to drive the magnet to move toward a direction close to the reaction chamber in the corresponding channel to be in a first position close to the reaction chamber in the corresponding channel, so that the magnetic field generated by the magnet covers the reaction liquid in the reaction chamber in the corresponding channel for magnetic adsorption;

[0021] The controlling the magnetic separation unit to stop generating a magnetic field for the reaction liquid in the reaction chamber in the corresponding channel to stop magnetic adsorption includes:

[0022] The control driving assembly drives the magnet to move in a direction away from the reaction chamber in the corresponding channel and to be in a second position away from the reaction chamber in the corresponding channel, so that the reaction liquid in the reaction chamber in the corresponding channel is located outside the coverage of the magnetic field generated by the magnet and stops magnetic adsorption.

[0023] Wherein, after the liquid injection mechanism is moved to inject the cleaning liquid in the liquid injection mechanism into the reaction chamber, the working method of the magnetic separation cleaning device further includes sucking out the cleaning liquid in the reaction chamber as waste liquid through the liquid suction mechanism.

[0024] Wherein, the cleaning liquid is introduced into the liquid injection mechanism at least three times.

[0025] The magnetic separation cleaning device of the present invention is provided with an incubation mechanism with multiple channels and multiple magnetic separation units, and each magnetic separation unit is provided corresponding to a channel in the incubation mechanism, so that each magnetic separation unit can work independently, each magnetic separation unit is not affected by each other, and can complete the operation of magnetic bead adsorption during the magnetic separation cleaning process of the reaction liquid in the reaction chamber in the corresponding channel. Each magnetic separation unit is arranged adjacent to the corresponding channel, so that the magnetic separation cleaning device has a compact structure, can save the installation space of the magnetic separation cleaning device, and is conducive to the miniaturization of the magnetic separation cleaning device such as analytical instruments. The magnetic separation cleaning device is also provided with a liquid suction mechanism and a liquid injection mechanism, which can successively perform waste liquid suction and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the corresponding channel, so as to complete the entire process of magnetic separation cleaning of the reaction liquid in the reaction chamber in the corresponding channel, so as to remove the unreacted reagents and samples in the reaction liquid, leaving a complex with magnetic beads as a solid phase carrier that can be specifically bound to the reagents required for the subsequent detection step. During the entire process, the position of the incubation mechanism is fixed, and the reaction liquid in the reaction chamber in the corresponding channel is magnetically separated and cleaned by driving the magnetic separation unit, the liquid aspiration mechanism, and the liquid injection mechanism, which is beneficial to saving the working time of the magnetic separation cleaning device and thus improving the working efficiency of the magnetic separation cleaning device. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the implementation mode will be briefly introduced below. Obviously, the drawings described below are only some implementation modes of the present invention. For ordinary technicians in this field, other drawings can be obtained like these drawings without paying creative work.

[0027] Figure 1 It is a schematic diagram of a magnetic separation and cleaning device provided in an embodiment of the present application from one angle;

[0028] Figure 2 yes Figure 1 A schematic diagram of the magnetic separation and cleaning device from another angle shown;

[0029] Figure 3 yes Figure 1 A schematic diagram of a state of the magnetic separation cleaning device shown;

[0030] Figure 4 yes Figure 1 Another schematic diagram of the magnetic separation and cleaning device shown in FIG.

[0031] Figure 5 It is a flow chart of the working method of the magnetic separation and cleaning device provided in this application. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] See also Figure 1 and Figure 2 The present invention provides a magnetic separation cleaning device 100, which includes an incubation mechanism 10, a fixing seat 20, a plurality of magnetic separation units 30, a liquid suction mechanism (not shown) and a liquid injection mechanism (not shown); the incubation mechanism 10 is arranged on one side of the fixing seat 20, and the incubation mechanism 10 has a plurality of channels 11, each of which is used to accommodate a reagent strip (not shown), the reagent strip has a reaction chamber, and the reaction chamber is used to accommodate a reaction liquid; a plurality of magnetic separation units 30 are arranged on the fixing seat 20 at intervals and each of the magnetic separation units 30 corresponds to one of the channels 11, each of the magnetic separation units 30 works independently and shares one liquid suction mechanism and one liquid injection mechanism, and each magnetic separation unit 30, the liquid suction mechanism, and the liquid injection mechanism are used to perform magnetic bead separation, waste liquid suction, and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the channel 11 corresponding to the magnetic separation unit 30 in sequence when performing magnetic separation cleaning on the reaction liquid in the reaction chamber in the channel 11 corresponding to the magnetic separation unit 30.

[0034] When it is necessary to measure a certain component in the sample, the corresponding antibody / antigen can be coated on the magnetic beads to form a magnetic bead reagent, and the specific marker can be labeled on the antibody to form a labeled reagent. It should be noted that the reagents for measuring a certain analytical item generally have multiple components, such as the magnetic bead reagent component and the labeled reagent component here, and the different components of the same item can be packaged in different cavities of the same reagent container or in different reagent containers. In the test process, the sample containing the object to be tested is first mixed with the magnetic bead reagent, the labeled reagent and other reagents in turn and incubated together under certain conditions to form a reaction solution; then, the unbound markers and other reagents and sample components in the reaction solution are removed by washing and separation technology; then a signal reagent is added thereto, and the marker on the reaction solution reacts with the signal reagent to emit light, wherein the signal reagent can be one or more, such as a luminescent substrate solution, a pre-excitation solution and an excitation solution, and a luminescence enhancement solution, etc., to complete a detection process. It can be understood that the reaction solution mentioned here is actually a reaction complex formed by mixing and incubating reagents and samples. In this embodiment, the magnetic separation and cleaning technology is also used as an example for explanation. That is, after the incubation reaction of the sample and the reagent is completed, it is necessary to clean and remove the unreacted sample and reagent, leaving only the complex with magnetic beads as the solid phase carrier after the reaction.

[0035] The magnetic separation cleaning device 100 is provided with an incubation mechanism 10 having a plurality of channels 11 and a plurality of magnetic separation units 30, and each magnetic separation unit 30 is provided corresponding to a channel 11 in the incubation mechanism 10, so that each magnetic separation unit 30 can work independently, each magnetic separation unit 30 is not affected by each other, and can complete the operation of magnetic bead adsorption during the magnetic separation cleaning process of the reaction liquid in the reaction chamber in the corresponding channel 11. Each magnetic separation unit 30 is arranged adjacent to the corresponding channel 11, so that the magnetic separation cleaning device 100 has a compact structure, can save the installation space of the magnetic separation cleaning device 100, and is conducive to the miniaturization of analytical instruments, etc., which use the magnetic separation cleaning device 100. The magnetic separation cleaning device 100 is also provided with a liquid suction mechanism and a liquid injection mechanism, which can sequentially perform waste liquid suction and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the corresponding channel 11, so as to complete the entire process of magnetic separation cleaning of the reaction liquid in the reaction chamber in the corresponding channel 11, thereby being able to remove unreacted reagents and samples in the reaction liquid, leaving behind a complex with magnetic beads as a solid phase carrier that can specifically bind to the reagents required for the subsequent detection steps. During the whole process, the position of the incubation mechanism 10 is fixed, and the reaction liquid in the reaction chamber in the corresponding channel 11 is magnetically separated and cleaned by driving the magnetic separation unit 30, the liquid suction mechanism, and the liquid injection mechanism, which is conducive to saving the working time of the magnetic separation cleaning device 100, thereby improving the working efficiency of the magnetic separation cleaning device 100.

[0036] In this embodiment, six channels 11 of the incubation mechanism 10 are used as an example for explanation, and six magnetic separation units 30 corresponding to the six channels 11 of the incubation mechanism 10 are also used as an example for explanation. Specifically, each of the channels 11 has a cavity seat 12, and the cavity seat 12 is located on the side of the incubation mechanism 10 close to the fixed seat 20. The cavity seat 12 is used to accommodate the cavity of the reaction chamber on the reagent strip, so that when the reagent strip is accommodated in the channel 11, the cavity of the reaction chamber can be embedded in the cavity seat 12, and because the cavity seat 12 is arranged close to the magnetic separation unit 30, the distance between the reaction chamber and the magnetic separation unit 30 is shortened, so that the reaction chamber can be closer to the magnetic separation unit 30, so that the magnetic separation unit 30 can more conveniently and quickly perform the operation of magnetic bead adsorption on the reaction liquid in the reaction chamber in the corresponding channel 11. In other embodiments, the number of channels of the incubation mechanism 10 and the number of magnetic separation units 30 may also be specifically set according to actual needs, as long as each magnetic separation unit 30 corresponds to one channel 11, and no limitation is made here.

[0037] Furthermore, each magnetic separation unit 30 includes a driving component 31 and a magnet 32, wherein the driving component 31 is used to drive the magnet 32 ​​to move in a direction away from or close to the reaction chamber in the corresponding channel 11, wherein when the magnet 32 ​​is in a first position close to the reaction chamber in the corresponding channel 11, magnetic bead separation operations and waste liquid suction operations can be performed under the action of the magnet 32, and when the magnet 32 ​​is in a second position away from the reaction chamber in the corresponding channel 11, liquid injection and cleaning operations can be performed.

[0038] For details, please refer to Figure 3, the magnet 32 ​​can be moved under the drive of the driving component 31. When the magnet 32 ​​moves to the first position close to the reaction chamber in the corresponding channel 11, the magnet 32 ​​adsorbs the magnetic beads on the reaction liquid in the reaction chamber in the corresponding channel 11. It should be noted that the magnetic beads are a complex with magnetic particles as solid phase carriers after reaction. While the magnet 32 ​​is maintaining the magnetic adsorption of the magnetic beads in the reaction liquid, the liquid suction mechanism absorbs the unreacted reaction liquid in the reaction chamber, leaving only the magnetic beads. When the magnet 32 ​​moves to the second position close to the reaction chamber in the corresponding channel 11, the liquid injection mechanism cleans the magnetic beads in the reaction chamber to complete the entire magnetic separation and cleaning process. During the entire magnetic separation and cleaning process, the position of the incubation mechanism 10 is fixed, and the magnetic separation cleaning device 100 can complete the magnetic adsorption operation during the magnetic separation and cleaning process only by moving the magnet 32 ​​in the magnetic separation unit 30. It has a simple structure and is easy to operate. The magnetic adsorption operation can be completed without moving the incubation mechanism 10, which is beneficial to saving the working time of the magnetic separation cleaning device 100, thereby improving the working efficiency of the magnetic separation cleaning device 100.

[0039] Please refer again Figure 1-Figure 3 , the fixed seat 20 includes a bottom plate 21, a first limit plate 22, a second limit plate 23 and a plurality of slide bars 24, the first limit plate 22 and the second limit plate 23 are arranged on opposite sides of the bottom plate 21, and the plurality of slide bars 24 are arranged between the first limit plate 22 and the second limit plate 23 at intervals, and the opposite ends of each slide bar 24 are respectively connected to the first limit plate 22 and the second limit plate 23, and the plurality of slide bars 24 are arranged in one-to-one correspondence with the plurality of magnetic separation units 30. In this embodiment, in order to correspond to six magnetic separation units 30, six slide bars 24 are used as an example to illustrate the plurality of slide bars 24. In other embodiments, the number of slide bars 24 can also be specifically set according to actual needs, as long as each magnetic separation unit 30 corresponds to one slide bar 24, and no limitation is made here.

[0040] Furthermore, the magnet 32 ​​of each magnetic separation unit 30 is slidably disposed on the corresponding slide rod 24 and is movably connected to the driving assembly 31 , and the incubation mechanism 10 is located on the side of the second limiting plate 23 away from the first limiting plate 22 .

[0041] Specifically, the magnet 32 ​​is an inverted "L" shape, which includes a first part 321 and a second part 322 connected to each other. In this embodiment, the first part 321 is the vertical part of the inverted "L" shape, and the first part 321 is slidably set on the sliding rod 24. The second part 322 is the horizontal part of the inverted "L" shape, and the second part 322 is used to resist the incubation mechanism 10.

[0042] Furthermore, the magnet 32 ​​has a first abutting end 323, which is formed by the end protrusion of the second part 322, and the incubation mechanism 10 has a second abutting end 101, which is formed by the side wall depression of the incubation mechanism 10 close to the second limiting plate 23. When the magnet 32 ​​moves to the first position, the first abutting end 323 abuts against the second abutting end 101, and the magnetic beads are adsorbed to the cavity wall of the reaction chamber close to the magnet 32 ​​by the magnetic attraction force of the magnet 32, and the magnetic separation unit 30 completes the operation of adsorbing the magnetic beads in the reaction liquid in the reaction chamber in the corresponding channel 11. In this embodiment, in order to achieve a connection relationship in which the magnet 32 ​​and the incubation mechanism 10 can abut against each other, the height of the second limiting plate 23 is smaller than the height of the first limiting plate 22, and the highest position of the second limiting plate 23 is also lower than the lowest position of the first abutting end 323, so that the second abutting end 101 of the magnet 32 ​​can cross the second limiting plate 23 and abut against the first abutting end 323 of the incubation mechanism 10.

[0043] In this embodiment, the driving component 31 is used to drive the magnet 32 ​​to move along the sliding rod 24 in a direction close to the incubation mechanism 10 and move to a first position close to the reaction chamber in the corresponding channel 11, or the driving component 31 is used to drive the magnet 32 ​​to move along the sliding rod 24 in a direction away from the incubation mechanism 10 and move to a second position away from the reaction chamber in the corresponding channel 11.

[0044] It is understandable that the driving assembly 31 can drive the magnet 32 ​​to reciprocate between the first position and the second position along the slide bar 24. Thus, when the magnet 32 ​​moves to the first position, the magnet 32 ​​adsorbs the reaction liquid in the reaction chamber in the corresponding channel 11, and at the same time, the liquid aspiration mechanism absorbs the unreacted reaction liquid in the reaction chamber, leaving only the magnetic beads. When the magnet 32 ​​moves to the second position close to the reaction chamber in the corresponding channel 11, the liquid injection mechanism cleans the magnetic beads in the reaction chamber to complete the entire magnetic separation cleaning process. The liquid injection cleaning operation is repeated multiple times by the liquid injection mechanism, and multiple liquid injection cleaning operations can strengthen the cleaning intensity and help improve the cleaning completion. For example, the liquid injection cleaning operation is repeated three times or more by the liquid injection mechanism.

[0045] Specifically, the driving assembly 31 includes an electrically connected driving motor 33 and a driving screw 34, the driving motor 33 is located on the side of the first limit plate 22 away from the second limit plate 23, the driving screw 34 is arranged between the first limit plate 22 and the second limit plate 23, and the opposite ends of the driving screw 34 are respectively connected to the first limit plate 22 and the second limit plate 23, the magnet 32 ​​is located on the driving screw 34, and the driving screw 34 is used to drive the magnet 32 ​​to move linearly along the driving screw 34 under the drive of the driving motor 33.

[0046] Further, the driving screw 34 is arranged corresponding to the slide bar 24, and the slide bar 24 is closer to the bottom plate 21 than the driving screw 34, that is, the driving screw 34 is arranged above the slide bar 24, and the stacked driving screw 34 and the slide bar 24 reduce the volume occupied by the magnetic separation unit 30, which is conducive to the miniaturization of the magnetic separation cleaning device 100. It can be understood that in this embodiment, the driving screw 34 is an actuator that converts rotational motion into linear motion.

[0047] In this embodiment, the magnetic separation cleaning device 100 also includes a first zero position detection plate 40, a first zero position sensor (not shown) and a controller (not shown), the first zero position detection plate 40 is arranged at the bottom of the base plate 21, and the first zero position detection plate 40 is arranged corresponding to the second limit plate 23, and the first zero position sensor is arranged on the first zero position detection plate 40.

[0048] In this embodiment, there are six first zero position sensors, each of which corresponds to one of the magnets 32, and the first zero position sensors are opposing optical coupling sensors. The bottom plate 21 is provided with a groove corresponding to each of the opposing optical coupling sensors, and the opposing optical coupling sensors are arranged on the first zero position detection plate 40 and are located in the groove. Specifically, the shape of the opposing optical coupling sensor resembles a "U" shape, and one of its electrodes emits a light beam to another electrode arranged oppositely. When the first zero position sensor is an opposing optical coupling sensor, a first baffle is correspondingly provided on the first part 321 of the magnet 32, and the driving motor drives the driving screw to drive the magnet to move and move to the first position, and when the first baffle moves to the two opposite electrodes of the opposing optical coupling sensor to block the emission of the light beam, it can be determined that the magnet is located in the first position and the magnetic bead separation operation and the waste liquid suction operation are performed. In other embodiments, the first zero position sensor can also be other sensors, for example, it can also be a Hall sensor.

[0049] Specifically, the driving motor 33 drives the driving screw 34 so that the magnet 32 ​​can move along the sliding rod 24 toward the incubation mechanism 10 and move to a first position close to the reaction chamber in the corresponding channel 11. When the magnet 32 ​​moves to the first position, the first zero position sensor is triggered to generate a first trigger signal. When the controller receives the first trigger signal, it determines that the magnet 32 ​​is located at the first position and controls the magnetic bead separation operation and the waste liquid suction operation.

[0050] That is, when the magnet 32 ​​is located in the first position, the first abutting end 323 of the magnet 32 ​​abuts against the second abutting end 101 of the incubation mechanism 10, and the magnet 32 ​​can perform magnetic bead adsorption operation on the reaction liquid in the reaction chamber in the corresponding channel 11, and while the magnet 32 ​​continues to provide magnetic adsorption force to the magnetic beads, the reaction liquid in the reaction chamber can be waste liquid absorbed through the liquid aspiration mechanism.

[0051] In this embodiment, the magnetic separation cleaning device 100 also includes a second zero position detection plate 50 and a second zero position sensor (not shown). The second zero position detection plate 50 is arranged on the top of the first limit plate 22, and the second zero position sensor is arranged on the second zero position detection plate 50.

[0052] In this embodiment, there are six second zero position sensors, each of which corresponds to one of the magnets 32, and the second zero position sensors are opposing optical coupling sensors. The opposing optical coupling sensors are arranged on the surface of the second zero position detection plate 50 close to the first limit plate 22. Specifically, the opposing optical coupling sensor resembles a "U" shape, and one electrode thereof emits a light beam to another electrode arranged oppositely. When the second zero position sensor is an opposing optical coupling sensor, a second baffle is correspondingly arranged on the second part 322 of the magnet 32, and the drive motor drives the drive screw to drive the magnet to move and move to the second position, and when the second baffle moves to between the two opposite electrodes of the opposing optical coupling sensor to block the emission of the light beam, it can be determined that the magnet is located in the second position and the liquid injection and cleaning operation is performed. In other embodiments, the second zero position sensor may also be other sensors, for example, it may also be a Hall sensor.

[0053] The drive motor 33 drives the magnet 32 ​​so that the magnet 32 ​​can move along the slide bar 24 in a direction away from the incubation mechanism 10 and move to a second position away from the reaction chamber in the corresponding channel 11. It can be understood that the second position can be the initial position of the magnet 32 ​​before the magnetic adsorption operation is performed, or the position to which the magnet 32 ​​returns when the magnetic adsorption operation is completed and the subsequent liquid injection and cleaning operation is to be performed, wherein the initial position and the return position are the same position.

[0054] When the magnet 32 ​​is reset to the second position, the second zero position sensor is triggered to generate a second trigger signal. When the controller receives the second trigger signal, it determines that the magnet 32 ​​is located at the second position and controls the subsequent liquid injection and cleaning operation. At this time, the magnet 32 ​​is away from the reaction chamber in the corresponding channel 11, and the liquid injection mechanism can perform liquid injection and cleaning operations on the reaction liquid in the reaction chamber.

[0055] That is, the driving motor 33 can drive the driving screw 34 to make the magnet 32 ​​extend or retract relative to the first limiting plate 22 along the sliding rod 24, so that the first abutting end 323 of the magnet 32 ​​abuts against or disengages from the second abutting end 101 of the incubation mechanism 10. When the magnet 32 ​​extends relative to the first limiting plate 22 along the sliding rod 24 and the first abutting end 323 of the magnet 32 ​​abuts against the second abutting end 101 of the incubation mechanism 10, the magnet 32 ​​is located at the first position; when the magnet 32 ​​retracts relative to the second limiting plate 23 along the sliding rod 24 and the second abutting end 101 of the magnet 32 ​​disengages from the second abutting end 101 of the incubation mechanism 10, the magnet 32 ​​is located at the second position.

[0056] Further, after the reaction liquid in the reaction chamber completes the liquid injection and cleaning operation, the magnetic beads in the reaction chamber are injected with an excitation liquid to make them emit light to complete the photometric operation. During the photometric operation, the magnet 32 ​​is located away from the reaction liquid in the reaction chamber. Preferably, the magnet 32 ​​is always located at the second position.

[0057] Please also read Figure 3 and Figure 4 It should be noted that, since each magnetic separation unit 30 can work independently and does not affect each other, the six magnetic separation units 30 can be: Figure 3 As shown, only the first abutting end 323 of the magnet 32 ​​in one of the magnetic separation units 30 abuts against the second abutting end 101 of the incubation mechanism 10, or as shown in FIG. Figure 4 As shown, the first abutting ends 323 of all the magnets 32 in the six magnetic separation units 30 abut against the second abutting ends 101 of the incubation mechanism 10 .

[0058] Furthermore, in this embodiment, before the magnetic separation cleaning device 100 performs the operation of magnetic bead adsorption through the magnetic separation unit 30, the liquid suction mechanism (not shown) is also used to suck up reagents and samples and inject the reagents and samples into the reaction chamber of the reagent strip (not shown) to form the reaction liquid. That is, the liquid suction mechanism can inject samples and reagents into the reaction chamber and incubate to form a reaction liquid, and can also suck the unreacted reaction liquid from the reaction chamber and discharge it as waste liquid. Using the same liquid suction mechanism to complete the suction and discharge operations helps to save the cost of materials required in the detection process, and also shortens the time required to replace other mechanisms to complete the corresponding operations, which is conducive to improving the efficiency of detection.

[0059] Furthermore, the liquid aspiration mechanism further includes a disposable liquid aspiration head, and after the liquid aspiration mechanism completes the operation of aspirating the reagent and sample on one of the reagent strips and the operation of aspirating the waste liquid by engaging the disposable liquid aspiration head, the disposable liquid aspiration head is discarded. In this embodiment, the operation of aspirating the waste liquid includes not only aspirating the unreacted reaction liquid as waste liquid for discharge, but also aspirating the cleaning liquid injected into the reaction chamber by the injection mechanism for discharge as waste liquid.

[0060] The magnetic separation cleaning device 100 of the present invention is provided with an incubation mechanism 10 having multiple channels 11 and multiple magnetic separation units 30, and each magnetic separation unit 30 is provided corresponding to a channel 11 in the incubation mechanism 10, so that each magnetic separation unit 30 can work independently, each magnetic separation unit 30 is not affected by each other, and can complete the operation of magnetic bead adsorption during the magnetic separation cleaning process of the reaction liquid in the reaction chamber in the corresponding channel 11. Each magnetic separation unit 30 is arranged adjacent to the corresponding channel 11, so that the magnetic separation cleaning device 100 has a compact structure, can save the installation space of the magnetic separation cleaning device 100, and is conducive to the miniaturization of analytical instruments such as the magnetic separation cleaning device 100. The magnetic separation cleaning device 100 is also provided with a liquid suction mechanism and a liquid injection mechanism, which can sequentially perform waste liquid suction and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the corresponding channel 11, so as to complete the entire process of magnetic separation cleaning of the reaction liquid in the reaction chamber in the corresponding channel 11, thereby being able to remove unreacted reagents and samples in the reaction liquid, leaving behind a complex with magnetic beads as a solid phase carrier that can specifically bind to the reagents required for the subsequent detection steps. During the whole process, the position of the incubation mechanism 10 is fixed, and the reaction liquid in the reaction chamber in the corresponding channel 11 is magnetically separated and cleaned by driving the magnetic separation unit 30, the liquid suction mechanism, and the liquid injection mechanism, which is conducive to saving the working time of the magnetic separation cleaning device 100, thereby improving the working efficiency of the magnetic separation cleaning device 100.

[0061] Please also read Figure 1 and Figure 5 The present invention also provides a working method of a magnetic separation cleaning device 100, wherein the magnetic separation cleaning device 100 comprises an incubation mechanism 10, a plurality of magnetic separation units 30, a liquid suction mechanism and a liquid injection mechanism, wherein the incubation mechanism 10 has a plurality of channels 11, each of the channels 11 is used to accommodate a reagent strip, the reagent strip has a reaction chamber, the reaction chamber is used to accommodate a reaction liquid, each of the magnetic separation units 30 corresponds to one of the channels 11, each of the magnetic separation units 30 works independently and shares one of the liquid suction mechanism and one of the liquid injection mechanism, the magnetic separation unit 30 is used to magnetically adsorb and terminate the magnetic adsorption of the reaction liquid, and the working method of the magnetic separation cleaning device 100 comprises:

[0062] S110: When controlling the magnetic separation unit 30 to generate a magnetic field for magnetic adsorption of the reaction liquid in the reaction chamber in the corresponding channel 11, the liquid suction mechanism is moved to absorb the waste liquid after the reaction in the reaction chamber is completed;

[0063] Specifically, each magnetic separation unit 30 includes a driving component 31 and a magnet 32, and the step S110 may specifically include: controlling the driving component 31 to drive the magnet 32 ​​to move toward the direction of the reaction chamber in the corresponding channel 11 and to be in the first position close to the reaction chamber in the corresponding channel 11, so that the magnetic field generated by the magnet 32 ​​covers the reaction liquid in the reaction chamber in the corresponding channel 11 and performs magnetic adsorption, and while performing magnetic adsorption, the liquid suction mechanism is moved to absorb the waste liquid after the reaction in the reaction chamber is completed. It can be understood that what is adsorbed in the magnetic adsorption is magnetic beads, which are complexes with magnetic particles as solid phase carriers after reaction.

[0064] S120: Control the magnetic separation unit 30 to stop generating a magnetic field for the reaction liquid in the reaction chamber in the corresponding channel 11 and stop magnetic adsorption;

[0065] Specifically, the step S120 may specifically include: controlling the driving component 31 to drive the magnet 32 ​​to move in a direction away from the reaction chamber in the corresponding channel 11 and to be in a second position away from the reaction chamber in the corresponding channel 11, so that the reaction liquid in the reaction chamber in the corresponding channel 11 is located outside the coverage of the magnetic field generated by the magnet 32, and the magnetic adsorption stops.

[0066] S130: introducing the cleaning liquid into the liquid injection mechanism;

[0067] Specifically, the cleaning liquid is introduced into the liquid injection mechanism at least three times. The cleaning liquid is used to remove the reaction liquid that has not participated in the reaction. Preferably, the cleaning liquid can be a pre-excitation liquid.

[0068] S140: Move the liquid injection mechanism to inject the cleaning liquid in the liquid injection mechanism into the reaction chamber.

[0069] Specifically, after S140, the cleaning liquid in the reaction chamber is sucked out as waste liquid through the liquid suction mechanism.

[0070] The working method of the magnetic separation cleaning device provided by the present invention is to control each magnetic separation unit 30 to magnetically adsorb the reaction liquid in the reaction chamber in the corresponding channel 11, move the liquid suction mechanism to absorb the waste liquid of the reaction liquid in the reaction chamber in the channel 11, and after controlling the magnetic separation unit 30 to stop magnetically adsorbing the reaction liquid in the reaction chamber in the corresponding channel 11, move the liquid injection mechanism to perform liquid injection cleaning on the reaction liquid in the reaction chamber in the corresponding channel 11, so as to complete the entire process of magnetic separation cleaning of the reaction liquid in the reaction chamber in the corresponding channel 11, so as to remove the unreacted reagents and samples in the reaction liquid, and leave the complex with magnetic beads as the solid phase carrier that can be specifically bound to the reagents required for the subsequent detection step. In the process of magnetic separation cleaning, the position of the incubation mechanism 10 is fixed, and only by controlling the magnetic separation unit 30 to start and stop magnetic adsorption, and while and after controlling the magnetic separation unit 30 to perform magnetic adsorption, the liquid suction mechanism and the liquid injection mechanism are moved to complete the process of waste liquid absorption and liquid injection cleaning of the reaction liquid in the reaction chamber in the corresponding channel 11, and the entire workflow is time-saving and labor-saving and has high work efficiency.

[0071] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A magnetic separation cleaning device, characterized in that: The magnetic separation cleaning device comprises an incubation mechanism, a fixed seat, a plurality of magnetic separation units, a liquid suction mechanism and a liquid injection mechanism; the fixed seat comprises a second limit plate and a plurality of sliding rods, and the plurality of sliding rods are arranged in a one-to-one correspondence with the plurality of magnetic separation units, the incubation mechanism is arranged on one side of the fixed seat and adjacent to the second limit plate, and the incubation mechanism has a plurality of channels, each of which is used to accommodate a reagent strip, and the reagent strip has a reaction chamber, and the reaction chamber is used to accommodate a reaction liquid; the plurality of magnetic separation units are arranged on the fixed seat at intervals and each of the magnetic separation units corresponds to a channel, and the magnetic separation units work independently and share a liquid suction mechanism and a liquid injection mechanism, and each magnetic separation unit comprises a driving assembly and a magnet, and the magnet of each magnetic separation unit is slidably arranged on the corresponding sliding rod and movably connected to the driving assembly, and the magnet is an inverted "L" shape and comprises a first part and a second part connected to each other, and the first part is the inverted The "L"-shaped vertical part, the first part is slidably arranged on the sliding rod, and the second part is the horizontal part of the inverted "L" shape, and the second part is used to abut against the incubation mechanism; the driving component is used to drive the magnet to move in the direction away from or close to the reaction chamber in the corresponding channel, the magnet has a first abutting end, and the first abutting end is formed by the end protrusion of the second part, the incubation mechanism has a second abutting end, the highest position of the second limiting plate is also lower than the lowest position of the first abutting end, and the first abutting end is used to move to the first position close to the reaction chamber in the corresponding channel under the drive of the driving component, cross the second limiting plate and abut against the second abutting end, each magnetic separation unit, the liquid suction mechanism, and the liquid injection mechanism are used to perform magnetic bead separation, waste liquid suction, and liquid injection cleaning operations on the reaction liquid in the reaction chamber in the corresponding channel in sequence when the reaction liquid in the reaction chamber in the channel corresponding to the magnetic separation unit is subjected to magnetic separation and cleaning.

2. The magnetic separation cleaning device according to claim 1, characterized in that: When the magnet is in a first position close to the reaction chamber in the corresponding channel, magnetic bead separation operations and waste liquid suction operations can be performed under the action of the magnet, and when the magnet is in a second position away from the reaction chamber in the corresponding channel, liquid injection and cleaning operations can be performed.

3. The magnetic separation cleaning device according to claim 2, characterized in that: The fixing seat comprises a first limiting plate, the first limiting plate and the second limiting plate are arranged opposite to each other, a plurality of the sliding rods are arranged between the first limiting plate and the second limiting plate at intervals, and opposite ends of each of the sliding rods are respectively connected to the first limiting plate and the second limiting plate; The incubation mechanism is located on the side of the second limit plate away from the first limit plate, and the driving component is used to drive the magnet to move along the sliding rod in a direction close to the incubation mechanism and move to a first position close to the reaction chamber in the corresponding channel, or the driving component is used to drive the magnet to move along the sliding rod in a direction away from the incubation mechanism and move to a second position away from the reaction chamber in the corresponding channel.

4. The magnetic separation cleaning device according to claim 3, characterized in that: The second abutting end is formed by a recessed side wall of the incubation mechanism close to the second limiting plate. When the magnet moves to the first position, the first abutting end abuts against the second abutting end, and the magnetic beads are adsorbed to the cavity wall of the reaction chamber close to the magnet by the magnetic attraction force of the magnet. The magnetic separation unit completes the operation of adsorbing the magnetic beads in the reaction liquid in the reaction chamber in the corresponding channel.

5. The magnetic separation cleaning device according to claim 3, characterized in that: The driving assembly includes an electrically connected driving motor and a driving screw, wherein the driving motor is located on the side of the first limit plate away from the second limit plate, the driving screw is arranged between the first limit plate and the second limit plate, and the opposite ends of the driving screw are respectively connected to the first limit plate and the second limit plate, the magnet is located on the driving screw, and the driving screw is used to drive the magnet to move linearly along the driving screw under the drive of the driving motor.

6. The magnetic separation cleaning device according to claim 3, characterized in that: The magnetic separation and cleaning device also includes a first zero position detection plate, a first zero position sensor and a controller. The first zero position detection plate is arranged at the bottom of the second limit plate, and the first zero position sensor is arranged on the first zero position detection plate. When the magnet moves to the first position, the first zero position sensor is triggered to generate a first trigger signal. When the controller receives the first trigger signal, it determines that the magnet is located at the first position and controls the magnetic bead separation operation and the waste liquid suction operation.

7. The magnetic separation cleaning device according to claim 6, characterized in that: The magnetic separation cleaning device also includes a second zero position detection plate and a second zero position sensor. The second zero position detection plate is arranged on the top of the first limit plate, and the second zero position sensor is arranged on the second zero position detection plate. When the magnet is reset to the second position, the second zero position sensor is triggered to generate a second trigger signal. When the controller receives the second trigger signal, it determines that the magnet is located at the second position and controls the subsequent liquid injection cleaning operation.

8. The magnetic separation cleaning device according to claim 1, characterized in that: Before the magnetic separation and cleaning device performs the operation of magnetic bead adsorption through the magnetic separation unit, the liquid aspiration mechanism is also used to aspirate reagents and samples and inject the reagents and samples into the reaction chamber of the reagent strip to form the reaction solution.

9. The magnetic separation cleaning device according to claim 1, characterized in that: The liquid injection and cleaning operation is repeated multiple times by the liquid injection mechanism.

10. A working method of a magnetic separation cleaning device, characterized in that: The magnetic separation cleaning device includes an incubation mechanism, a fixed seat, a plurality of magnetic separation units, a liquid suction mechanism and a liquid injection mechanism. The fixed seat includes a second limit plate and a plurality of sliding rods. The plurality of sliding rods are arranged in a one-to-one correspondence with the plurality of magnetic separation units. The incubation mechanism is arranged on one side of the fixed seat and is arranged adjacent to the second limit plate. The incubation mechanism has a plurality of channels, each of which is used to accommodate a reagent strip, and the reagent strip has a reaction chamber, and the reaction chamber is used to accommodate a reaction liquid. A plurality of magnetic separation units are arranged on the fixed seat at intervals and each of the magnetic separation units corresponds to a channel. The magnetic separation units work independently and share a liquid suction mechanism and a liquid injection mechanism. Each magnetic separation unit includes a driving assembly and a magnet. The magnet of each magnetic separation unit is slidably arranged on the corresponding The cam is mounted on a slide bar and is movably connected to the drive assembly, the magnet is in an inverted "L" shape and includes a first part and a second part that are connected, the first part being the vertical part of the inverted "L" shape, the first part being slidably arranged on the slide bar, the second part being the horizontal part of the inverted "L" shape, and the second part being used to abut against the incubation mechanism; the magnet has a first abutting end, the first abutting end is formed by a protrusion of the end of the second part, the incubation mechanism has a second abutting end, the highest position of the second limiting plate is also lower than the lowest position of the first abutting end, the drive assembly is used to drive the magnet to move in a direction away from or close to the reaction chamber in the corresponding channel, the magnetic separation unit is used to magnetically adsorb and terminate the magnetic adsorption of the reaction liquid, and the working method of the magnetic separation cleaning device includes: Controlling the driving assembly to drive the magnet to move toward a direction close to the reaction chamber in the corresponding channel and to be in a first position close to the reaction chamber in the corresponding channel, so that the magnetic field generated by the magnet covers the reaction liquid in the reaction chamber in the corresponding channel for magnetic adsorption, wherein the first abutting end passes over the second limiting plate and abuts against the second abutting end; Control the driving assembly to drive the magnet to move in a direction away from the reaction chamber in the corresponding channel to a second position away from the reaction chamber in the corresponding channel, so that the reaction liquid in the reaction chamber in the corresponding channel is located outside the coverage of the magnetic field generated by the magnet, and the magnetic adsorption stops; Introducing cleaning liquid into the liquid injection mechanism; The liquid injection mechanism is moved to inject the cleaning liquid in the liquid injection mechanism into the reaction chamber.

11. The working method of the magnetic separation cleaning device according to claim 10, characterized in that: After the liquid injection mechanism is moved to inject the cleaning liquid in the liquid injection mechanism into the reaction chamber, the working method of the magnetic separation cleaning device further includes sucking out the cleaning liquid in the reaction chamber as waste liquid through the liquid suction mechanism.

12. The working method of the magnetic separation cleaning device according to claim 10, characterized in that: The cleaning liquid is introduced into the liquid injection mechanism at least three times.

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