Magnetic bead cleaning device and method capable of adding luminescent substrate

Abstract

The present invention is a magnetic bead cleaning device and method capable of filling a luminescent substrate, comprising a magnetic bead cleaning mechanism with a lifting needle rack, the device also comprising a substrate filling mechanism, the forward, backward, upward and downward movements required by the substrate filling mechanism are synchronously completed with the upward and downward movements of the lifting needle rack, the liquid line pipe outlet of the substrate filling mechanism performs a filling operation when the lifting needle rack is located at the upper limit position, and the liquid line pipe outlet of the substrate filling mechanism performs substrate filling when the lifting needle rack is located at the lower limit position. It can realize the normal magnetic bead cleaning action while also meeting the requirements of the downward exploration filling of the luminescent substrate (reagents, diluents and other liquids) and the independent pipeline filling position and action. Under the premise of ensuring the functionality of magnetic bead cleaning and separation and substrate filling and filling, the substrate is driven to fill downward and fill during the liquid suction action and to perform the filling action during the rise, so as to achieve a simple structure, high integration, multifunctionality, stability and improve the operation speed.

Description

Technical Field

[0001] The invention relates to a magnetic bead cleaning device and method capable of adding a luminescent substrate. Background Art

[0002] Chemiluminescent enzyme immunoassay is a commonly used analytical method for chemiluminescence instruments. It uses labeled antigens or antibodies to form a solid-phase coated antibody-antigen-enzyme labeled antibody complex after an immune reaction with the corresponding antigen (antibody) in the sample to be tested. After washing with magnetic beads, luminescent substrates are added, and the enzyme catalyzes and decomposes the substrate to emit light, which is received by the photon reading system. The photomultiplier tube converts the light signal into an electrical signal and amplifies it, and then transmits it to the computer data processing system to calculate the concentration of the measured object.

[0003] Among them, magnetic bead cleaning and filling of luminescent substrate are two indispensable and important processes. In order to meet the above processes, the general technology is a set of special magnetic bead cleaning equipment and a set of substrate filling equipment. The magnetic bead cleaning and the filling of luminescent substrate are completed by two sets of independent complex mechanisms, which makes the product structure complex, difficult to control, large space occupied, high cost, difficult to maintain and other defects; or just pre-set a substrate filling position in the magnetic bead cleaning device for substrate filling.

[0004] For example, the invention patent with the publication number: CN 110125075 A discloses a magnetic bead cleaning and separation device. Although a substrate injection port is added, its working method is as follows: the transfer plate transports the reaction cup containing the magnetic beads to the corresponding operating position, which includes the liquid injection position, the liquid discharge position and the substrate filling position, to clean the magnetic beads and fill the substrate; this method simply adds an operating position for substrate filling. In actual work, since substrates of different batches and models need to be replaced during normal use, and the residual substrate in the pipeline may affect the test, the pipeline needs to be filled and flushed. This method can only use the reaction cup to spit liquid during flushing, and rotate it to the liquid suction position for discharge. Due to the limited capacity of the reaction cup, multiple steps need to be repeated, wasting precious time in the test. At the same time, the filling position is above the reaction cup. In order to prevent the substrate from splashing onto the cup wall during filling, the position needs to be equipped, which increases the difficulty of installation. Summary of the invention

[0005] In view of the shortcomings of the prior art, the technical problem to be solved by the present invention is to provide a device and method that can realize the normal magnetic bead washing action while also satisfying the downward injection of luminescent substrates (reagents, diluents and other liquids) and independent pipeline flushing positions and actions. On the premise of ensuring the functionality of magnetic bead washing and separation and substrate injection and flushing, the substrate is driven to inject downwards and perform flushing actions when the liquid is sucked, so as to achieve a simple structure, high integration, multifunctionality, stability and improve the operating speed.

[0006] The technical solution adopted by the present invention to solve the technical problem is: to provide a magnetic bead cleaning device that can be filled with a luminescent substrate, including a magnetic bead cleaning mechanism with a lifting needle rack, characterized in that the device also includes a substrate filling mechanism, the forward, backward, up and down movements required by the substrate filling mechanism are completed synchronously with the up and down movements of the lifting needle rack, the liquid circuit pipe outlet of the substrate filling mechanism performs a flushing operation when the lifting needle rack is located at the upper limit position, and the liquid circuit pipe outlet of the substrate filling mechanism performs substrate filling when the lifting needle rack is located at the lower limit position.

[0007] The substrate filling mechanism comprises a linear guide rail, a guide rail, a connecting arm, and a liquid spouting pool. A substrate filling position is arranged on the circumference of the cleaning disk of the magnetic bead cleaning mechanism, and a filling and spouting position is arranged on the surface of the cleaning disk inside the substrate filling position. The liquid spouting pool is fixedly mounted on the surface of the cleaning disk through a support plate and a liquid spouting pool fixing plate above the filling and spouting position.

[0008] The guide track is also fixed to the surface of the cleaning disk through a support plate, and a track groove is provided in the guide track, and the horizontal distance between the highest point of the track groove and the lowest point of the track groove is not less than the center distance between the injection and dispensing position and the substrate filling position;

[0009] The linear guide rail is horizontally fixed to the lower surface of the lifting needle holder through the guide rail connecting plate, and the moving direction of the linear guide rail is from the substrate injection position to the irrigation and dispensing position, which is defined as the front-to-back direction;

[0010] The side surface of one end of the connecting arm slides in the track groove through a ball bearing and a connecting shaft; the upper surface of the connecting arm is fixed together with a slider on the linear guide rail, and can move forward and backward along the linear guide rail under the action of the slider; the connecting arm always remains in a horizontal state, and the other end of the connecting arm is connected to a substrate needle, a liquid tube connects the substrate needle and a lifting needle rack, the substrate needle is inserted out of the outlet of the liquid tube, and the substrate injection liquid is injected through the liquid tube; the length of the connecting arm must ensure that when the lifting needle rack is located at the upper limit position, the outlet of the liquid tube is located above the liquid spitting pool, and the liquid in the liquid tube can just fall into the liquid spitting pool. Since the opening of the liquid spitting pool is large, the outlet of the liquid tube does not have to be directly opposite the center of the liquid spitting pool, and can be eccentric, as long as the liquid is not sprayed outside the liquid spitting pool.

[0011] The end of the liquid spouting pool is provided with a liquid outlet, which is connected to the waste liquid bucket through a corresponding pipeline. The upper end of the liquid spouting pool is in a horizontal state, and the overall installation height of the liquid spouting pool does not interfere with the forward and backward, up and down movement of the substrate needle on the liquid line pipe. The liquid spouting pool as a whole can be an irregular shape such as a boot to meet the installation space requirements of the cleaning device.

[0012] The substrate needle includes a stainless steel tube 111, a horizontal connecting bracket 114 and a locking joint 13. The locking joint is connected to the top of the stainless steel tube, and a locking threaded hole is opened in the locking joint. The upper part of the stainless steel tube is a smooth transition structure 112, and the lower part of the stainless steel tube is a vertical structure. The smooth transition structure has a certain curvature and can smoothly connect the vertical structure and the locking joint to prevent rigid bending of the pipeline during the forward and backward movement, thereby preventing damage to the pipeline; the horizontal connecting bracket is fixedly connected to one end of the connecting arm by bolts, and the horizontal connecting bracket is welded to the outer wall of the smooth transition structure part of the stainless steel tube at the same time. One end of the liquid tube passes through the locking joint and extends into the stainless steel tube and extends out of the end of the vertical structure of the stainless steel tube for a distance, generally 2-3mm, to avoid contaminating the stainless steel tube; the other end of the liquid tube extends out of the lifting needle holder and is connected to the substrate storage bottle through an external liquid syringe and related valve groups. At this time, the distance from the center of the vertical structure of the stainless steel pipe to the highest point of the connection position of the connecting arm and the guide rail is not less than the distance from the inner edge of the spitting pool to the highest point, and is not greater than the distance from the outer edge of the spitting pool to the highest point. The inner and outer sides of the spitting pool refer to the side close to the guide rail and the side away from the guide rail, respectively. At this time, the spitting pool has two vertical planes, front and back.

[0013] The intersection of the liquid tube and the lifting needle frame is locked by a liquid tube locking joint, and after the position of the liquid tube is adjusted, it is locked by a liquid tube locking joint 13 to prevent the liquid tube from moving. The connection position of the liquid tube and the substrate needle 11 is also connected and locked by a locking joint, and after the length of the liquid tube 12 is adjusted, it is locked to prevent the liquid tube 12 from moving, and the outlet position of the liquid tube is limited.

[0014] The present invention also protects a magnetic bead cleaning method that can be filled with a luminescent substrate, and the process of the method is:

[0015] 1) Cleaning the magnetic beads in the reaction cup. After the cleaning action is completed, the magnetic beads in the reaction cup 17 continue to rotate clockwise through a reaction cup position every fixed period to reach the designated position;

[0016] 2) The lifting needle frame 3 is driven by the lead screw lifting mechanism 2 to descend again to clean the reaction cup magnetic beads. When the lifting needle frame is lowered to the right position again, the outlet of the liquid pipe 12 in the substrate filling mechanism is just located at the hole of the reaction cup that has completed the magnetic bead cleaning and penetrates into the reaction cup, and the liquid circuit is controlled to complete the action of injecting the luminescent substrate into the reaction cup that has completed the magnetic bead cleaning; when the lifting needle frame 3 is raised to the right position again, at this time, the outlet of the liquid pipe 12 of the substrate filling mechanism is located above the hole of the liquid discharging pool 14 of the substrate filling mechanism, and the liquid discharging and filling operations are completed;

[0017] 3) After the substrate is filled, the reaction cup rotates one hole position clockwise to reach the tube removal position, and the operating mechanism takes away the reaction cup filled with the substrate and enters the next process.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention is an improvement on the existing magnetic bead cleaning device. A substrate filling mechanism is added to the existing device. When the existing magnetic bead cleaning device is in normal operation, a set of substrate filling mechanisms is integrated into the magnetic bead cleaning device. The power of the existing magnetic bead cleaning device is used to complete the motion of substrate downward injection and independent filling and flushing, and the power device and control device system required for the independent substrate filling mechanism can be omitted. The independent substrate filling mechanism can be eliminated and its function can be fully integrated into the cleaning device, so that the function of substrate downward injection and independent pipeline filling of the reaction cup can be completed during the normal operation of the cleaning device.

[0020] The present invention utilizes the necessary up and down movement of the cleaning needle of the magnetic bead cleaning mechanism, and realizes the forward, backward, upward and downward movement required by the substrate filling mechanism through the combination of the provided guide track and the linear guide motion system, thereby combining the magnetic bead cleaning mechanism and the substrate filling device into one device, which satisfies multiple functions at the same time.

[0021] The cleaning device of the present invention has low manufacturing cost, simple structure, convenient assembly, optimized removal of substrate filling power device and corresponding control system, simple process and control, more efficient space utilization, more compact whole machine, high integration, and only one module needs to be maintained in the later stage, which is simple to maintain.

[0022] The substrate filling mechanism of the present invention can follow the up and down movement of the existing cleaning device to complete the forward and backward and up and down movement, realize the action of probing and filling, avoid the existing substrate filling device to increase the operating speed, need to set up an independent filling and flushing liquid spitting pool to quickly discharge the residual liquid of the pipeline filling, and avoid the splashing phenomenon that may be caused when the filling position is higher than the reaction cup, resulting in inaccurate filling amount and affecting the test results. At the same time, it also avoids the problem of frequently switching the position of the filling needle and probing to increase the complexity and difficulty of the structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the magnetic bead cleaning device capable of adding luminescent substrates according to the present invention.

[0024] Figure 2 It is a structural schematic diagram of the relevant components installed on the lifting needle holder of the present invention.

[0025] Figure 3 It is an exploded view of the installation method of the guide rail and the connecting arm in the present invention.

[0026] Figure 4 This is a schematic diagram of the relative positions of the guide rail, the connecting arm, and the liquid pipe when the substrate is filled in the present invention.

[0027] Figure 5 The figure is a schematic diagram of the relative positions of the guide rail, the connecting arm, the liquid pipe and the liquid discharging pool during the substrate pipeline irrigation and liquid discharging of the present invention.

[0028] Figure 6 It is a schematic diagram of the working position of the cleaning plate hole of the present invention.

[0029] Figure 7 This is a schematic diagram of the substrate filling and flushing principle in the present invention.

[0030] Figure 8 It is a schematic diagram of the horizontal cross-section and top view of the installation position of the dispensing pool in the present invention.

[0031] Fig. 9 Schematic diagram of the substrate needle structure of the present invention.

[0032] Fig.10 It is a schematic diagram of the fixing structure of the liquid dispensing pool and the guide track in the present invention.

[0033] Fig.11 It is a schematic structural diagram of the substrate needle of the present invention at another angle.

[0034] In the figure, there are a magnetic bead cleaning mechanism 1, a screw lifting mechanism 2, a lifting needle holder 3, a guide rail connecting plate 4, a linear guide rail 5, a guide rail 6, a rolling bearing 7, a connecting arm 8, a supporting plate 9, a connecting shaft 10, a substrate needle 11, a liquid line tube 12, a locking joint 13, a liquid discharging pool 14, a liquid aspiration needle 15, a liquid discharging needle 16, a reaction cup 17, a cleaning tray 18, a limit baffle 19, and a liquid discharging pool fixing plate 20. DETAILED DESCRIPTION

[0035] like Figure 1-4 As shown, the magnetic bead cleaning device that can be filled with a luminescent substrate in this embodiment includes a magnetic bead cleaning mechanism 1 and a substrate filling mechanism. The magnetic bead cleaning mechanism includes a screw lifting mechanism 2, a lifting needle rack 3, a liquid aspiration needle 15, a liquid discharging needle 16, a reaction cup 17, a cleaning tray 18, etc. The substrate filling mechanism includes a guide rail connecting plate 4, a linear guide rail 5, a guide rail 6, a rolling bearing 7, a connecting arm 8, a support plate 9, a connecting shaft 10, a substrate needle 11, a liquid line tube 12, a locking joint 13, a liquid discharging pool 14, a limit baffle 19, and a liquid discharging pool fixing plate 20.

[0036] The upper end of the guide rail connecting plate 4 is fixed to the lower end of the lifting needle frame 3, and the lower end is fixed to the linear guide rail 5; the lower surface of the support plate 9 is fixed on the cleaning disk of the magnetic bead cleaning mechanism 1 (such as Figure 8 guide rail 6 and the spitting pool 14, the spitting pool fixing plate 20 is mounted on the upper surface of the support plate 9 (such as Fig.10As shown in the figure, the liquid dispensing pool fixing plate 20 is L-shaped, fixed to the support plate on one hand, and fixed to the side of the liquid dispensing pool on the other hand, so that the guide track 6 and the liquid dispensing pool 14 form a relatively fixed whole with the magnetic bead cleaning mechanism 1; the linear guide rail 5 is horizontally fixed to the lower surface of the lifting needle frame 3 through the guide rail connecting plate 4, and the limit baffles 19 are installed at the front and rear ends of the linear guide rail 5. The guide track 6 is connected to the connecting arm 8 through the rolling bearing 7 and the connecting shaft 10. A track groove is provided on the side of the guide track 6. The rolling bearing 7 is installed in the track groove and is rollingly connected to the track groove. One end of the connecting shaft 10 is fixed to the rolling bearing 7, and the other end is fixed to the side of one end of the connecting arm 8. The connecting arm 8 can drive the rolling bearing 7 to slide along the guide track 6, as shown in the figure. Figure 3 As shown, the rolling bearing 7 is assembled into the track groove of the guide track. The use of rolling bearings can reduce friction resistance, making the movement smoother and reducing the noise generated during movement. The upper surface of the connecting arm 8 is also fixed to the slider of the linear guide 5. At this time, the connecting arm 8 can slide back and forth along the installation direction of the linear guide. Due to the presence of the limit baffle 19, it is prevented from detaching from the linear guide 5 when sliding. The connecting arm 8 always remains in a horizontal state during installation. A substrate needle 11 is fixed to one end of the connecting arm away from the guide track 6. A liquid tube 12 is inserted into the substrate needle 11. One end of the liquid tube 12 passes through the lifting needle frame 3. The junction between the liquid tube and the lifting needle frame 3 is locked by the liquid tube locking joint 13. After the position of the liquid tube is adjusted, it is locked by the locking joint 13 to prevent the liquid tube from moving. The substrate needle 11 (such as Fig. 9The stainless steel pipe 111 includes a stainless steel pipe 111, a horizontal connection bracket 114 and a locking joint 13. The stainless steel pipe has a guiding effect on the liquid pipe. The top of the stainless steel pipe is connected to the locking joint. A locking threaded hole is provided in the locking joint. The upper part of the stainless steel pipe is a smooth transition structure 112. The lower part of the stainless steel pipe is a vertical structure. The smooth transition structure has a certain curvature and can smoothly connect the vertical structure and the locking joint. Due to the smooth connection of the two parts, the structure is composed of two sections with different curvatures. The stainless steel pipe provides rigid support for the liquid pipe and a smooth transition structure 112 and a locking joint are provided on the structure. The threaded hole can be connected and locked with the locking joint. After adjusting the length of the liquid tube 12, it can be locked to prevent the liquid tube 12 from moving and to prevent the tube from being rigidly bent. The outlet position of the liquid tube is limited. The outlet of the liquid tube should extend a certain distance from the end of the vertical structure of the stainless steel tube, so that the substrate filling liquid only flows out of the liquid tube; the rolling bearing is placed in the track groove of the guide track 6; the lifting needle rack 3 is driven by the screw lifting mechanism 2. During the lifting process, the linear guide 5 and the guide track 6 are combined to plan a specified motion trajectory, and the connecting arm 8 moves back and forth along the motion trajectory. The upper and lower highest point positions of the connecting arm are controlled by the lifting requirements of the screw lifting mechanism itself in the existing magnetic bead cleaning mechanism. The connecting arm will move downward along the guide track under the action of the screw lifting mechanism, and move forward along the linear guide. When the screw lifting mechanism 2 drops to the specified position, the outlet end of the liquid tube is located at the hole position of the reaction cup to be filled with the luminescent substrate and probes into the reaction cup (such as Figure 4 As shown in the figure, the luminescent substrate can be added into the reaction cup 17 through the substrate needle by controlling the syringe (or quantitative pump) and valve group (not shown in the figure, which is the existing conventional equipment of the liquid line tube) of the liquid line, so as to avoid splashing on the wall; the lifting needle frame 3 is driven by the screw lifting mechanism, and when it rises, the connecting arm will move upward along the guide track, and move backward under the action of the linear guide rail. When it rises to the specified position, the outlet end of the liquid line tube is located at the upper end of the liquid spouting pool hole (as shown in the figure). Figure 5 As shown), the waste liquid or irrigation liquid is discharged into the liquid dispensing pool through the syringe (or metering pump) and valve group that control the liquid circuit, and discharged through the waste liquid pipeline connected to the lower end of the liquid dispensing pool.

[0037] The track groove of the guide track has a vertical section and an inclined section inclined upward, and the two have a smooth transition. The angle between the inclined section and the horizontal plane is 30°-45°.

[0038] The inner diameter of the end outlet of the vertical structure of the stainless steel tube is slightly smaller than the inner diameter of the upper inlet of the vertical structure. The inner diameter of the end outlet of the vertical structure is 1.8 mm, and the inner diameter of the upper inlet of the vertical structure is 2.0 mm, which is convenient for inserting the transition part of the stainless steel tube. The slightly smaller inner diameter of the end outlet can limit the angle of the stainless steel tube outlet, avoid skewing, and is not prone to splashing.

[0039] The basic function of the magnetic bead cleaning mechanism is that the lifting needle frame 3 is driven by the screw lifting mechanism 2 to perform a vertical lifting movement. During the descent process, the liquid dispensing needle 16 and the liquid aspiration needle 15 fixed on the lifting needle frame 3 are used to inject and absorb the cleaning liquid into the reaction cup 17 to complete the cleaning of the magnetic beads in the reaction cup; after the cleaning action is completed, the lifting needle frame 3 is lifted by the screw lifting mechanism. When it reaches the specified position, the screw lifting mechanism 2 stops. At this time, the liquid aspiration needle 15 is higher than the cleaning disk. Every fixed period, the cleaning disk 18 rotates clockwise through a reaction cup position; at this time, the lifting needle frame continues to descend to complete the cleaning of the magnetic beads in the reaction cup.

[0040] With the help of the magnetic bead cleaning mechanism, the magnetic beads in the reaction cup 17 complete the cleaning action and continue to rotate clockwise through a reaction cup position every fixed period to reach the specified position. The lifting needle frame 3 is driven by the screw lifting mechanism 2 to descend again to clean the reaction cup magnetic beads. Figure 4 As shown, the outlet of the liquid pipe 12 is located at the hole of the reaction cup where the magnetic beads have been cleaned and extends downward into the reaction cup. Through the liquid circuit control, the action of injecting the luminescent substrate into the reaction cup is completed. When the lifting needle frame 3 rises to the specified position (such as Figure 5 As shown in the figure, at this time, the outlet of the liquid pipe 12 is located above the hole position of the liquid dispensing pool 14, and the processes of dispensing, filling and flushing can be completed. At this time, the reaction cup that has completed the substrate filling rotates one hole position clockwise to reach the tube removal position, and the operating mechanism (which can be directly implemented by existing conventional technologies, such as a manipulator, etc.) takes away the reaction cup that has been filled with the substrate and enters the next process.

[0041] Figure 6 The figure is a schematic diagram of the relative positions of different workstations on the cleaning disk. The substrate injection position provided in the present invention is adjacent to the tube taking position. The filling, flushing and spitting position is provided on the cleaning disk behind the substrate injection position. The substrate injection position and the filling, flushing and spitting position are provided front and back, which is consistent with the moving direction of the provided linear guide rail.

[0042] In summary, through simple and ingenious structural design and methods, utilizing the original functional actions and power device of the magnetic bead cleaning mechanism, both the downward exploration and filling function of the luminescent substrate and the independent pipeline flushing function can be completed, thus forming a multifunctional magnetic bead cleaning device that can be filled with a luminescent substrate.

[0043] Any matters not described in the present invention are applicable to the prior art.

Claims

1. A magnetic bead cleaning device capable of being filled with a luminescent substrate, comprising a magnetic bead cleaning mechanism with a lifting needle holder, characterized in that: The device also includes a substrate filling mechanism, the forward, backward, upward and downward movements required by the substrate filling mechanism are completed synchronously with the upward and downward movements of the lifting needle frame, the liquid pipe outlet of the substrate filling mechanism performs a filling operation when the lifting needle frame is located at the upper limit position, and the liquid pipe outlet of the substrate filling mechanism performs substrate filling when the lifting needle frame is located at the lower limit position; The substrate filling mechanism comprises a linear guide rail, a guide rail, a connecting arm, and a liquid spouting pool. A substrate filling position is arranged on the circumference of the cleaning disk of the magnetic bead cleaning mechanism, and a filling and spouting position is arranged on the surface of the cleaning disk inside the substrate filling position. The liquid spouting pool is fixedly mounted on the surface of the cleaning disk through a support plate and a liquid spouting pool fixing plate above the filling and spouting position. The guide track is also fixed to the surface of the cleaning disk through a support plate, and a track groove is provided in the guide track, and the horizontal distance between the highest point of the track groove and the lowest point of the track groove is not less than the center distance between the injection and dispensing position and the substrate filling position; The linear guide rail is horizontally fixed to the lower surface of the lifting needle frame through the guide rail connecting plate, and the moving direction of the linear guide rail is from the substrate injection position to the irrigation and dispensing position; The side surface of one end of the connecting arm slides in the track groove through a ball bearing and a connecting shaft; the upper surface of the connecting arm is fixed to the slider on the linear guide rail, and can move forward and backward along the linear guide rail under the action of the slider; the other end of the connecting arm is connected to a substrate needle, a liquid line tube connects the substrate needle and the lifting needle rack, the substrate needle is inserted out of the outlet of the liquid line tube, and the substrate injection liquid is injected through the liquid line tube; the length of the connecting arm is to ensure that when the lifting needle rack is at the upper limit position, the outlet of the liquid line tube is located above the liquid dispensing pool, and the liquid in the liquid line tube can just fall into the liquid dispensing pool; The end of the liquid spouting pool is provided with a liquid outlet, which is connected to the waste liquid bucket through a corresponding pipeline. The upper end of the liquid spouting pool is in a horizontal state, and the overall installation height of the liquid spouting pool does not interfere with the forward and backward, up and down movement of the substrate needle on the liquid line pipe; The track groove of the guide track comprises a vertical section and an inclined section inclined upward, and the two are smoothly transitioned.

2. The cleaning device according to claim 1, characterized in that: The spitting pool is in the shape of a boot as a whole.

3. The cleaning device according to claim 1, characterized in that: The substrate needle includes a stainless steel tube, a horizontal connecting bracket and a locking joint. The locking joint is connected to the top of the stainless steel tube, and a locking threaded hole is opened in the locking joint. The upper part of the stainless steel tube is a smooth transition structure, and the lower part of the stainless steel tube is a vertical structure. The smooth transition structure can smoothly connect the vertical structure and the locking joint; the horizontal connecting bracket is fixedly connected to one end of the connecting arm by a bolt, and the horizontal connecting bracket is welded to the outer wall of the smooth transition structure part of the stainless steel tube at the same time. One end of the liquid tube passes through the locking joint and extends into the stainless steel tube and extends out of the vertical structure end of the stainless steel tube; the other end of the liquid tube extends out of the lifting needle rack and is connected to the substrate storage bottle through an external liquid syringe and related valve group.

4. The cleaning device according to claim 3, characterized in that: The distance from the center of the vertical structure of the stainless steel pipe to the highest point of the connection position between the connecting arm and the guide rail is not less than the distance from the inner edge of the spitting pool to the highest point, and is not greater than the distance from the outer edge of the spitting pool to the highest point.

5. The cleaning device according to claim 1, characterized in that: The intersection of the liquid tube and the lifting needle rack is locked by a liquid tube locking joint, and the liquid tube position is adjusted and locked by the liquid tube locking joint; the connection position of the liquid tube and the substrate needle is also connected and locked by a locking joint, and the liquid tube length is adjusted and locked to prevent the liquid tube from moving, thereby limiting the outlet position of the liquid tube.

6. The cleaning device according to claim 3, characterized in that: The inner diameter of the terminal outlet of the vertical structure of the stainless steel tube is smaller than the inner diameter of the upper inlet of the vertical structure, and the inner diameter of the terminal outlet of the vertical structure is 1.8 mm.

7. A method for cleaning magnetic beads that can be filled with a luminescent substrate, characterized in that: The process of this method is: 1) Cleaning the magnetic beads in the reaction cup. After the cleaning action is completed, the magnetic beads in the reaction cup continue to rotate clockwise or counterclockwise through a reaction cup position at a fixed period to reach the designated position; 2) The lifting needle rack is driven by the lead screw lifting mechanism to descend again to clean the reaction cup magnetic beads. When the lifting needle rack is lowered to the right position again, the outlet of the liquid pipe in the substrate filling mechanism is just located at the hole of the reaction cup that has completed the magnetic bead cleaning and penetrates into the reaction cup, and the liquid circuit is controlled to complete the action of injecting the luminescent substrate into the reaction cup that has completed the magnetic bead cleaning; when the lifting needle rack is raised to the right position again, at this time, the outlet of the liquid pipe of the substrate filling mechanism is located above the hole of the liquid spouting pool of the substrate filling mechanism, and the liquid spouting and filling operations are completed; 3) After the substrate is filled, the reaction cup is rotated one hole position clockwise or counterclockwise to reach the tube removal position, and the operating mechanism takes away the reaction cup filled with the substrate and enters the next process.

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