A flow fluorescence detection liquid path system

Abstract

The invention discloses a flow type fluorescence detection liquid circuit system, aiming to overcome the problem of low cleaning efficiency in the prior art. The flow chamber comprises a flow chamber, wherein the flow chamber has a sheath liquid inlet, a sample inlet and a waste liquid outlet, wherein the sheath liquid inlet is connected to a pulsation damper through a fifth control valve, the sample inlet is connected to a sampling device through a seventh control valve, the waste liquid outlet is connected to a waste liquid bottle, a cleaning device is arranged on the sampling device, and the first control valve is also included. The first control valve has a first inlet and two first outlets, one first outlet is connected to the pulsation damper, and the other first outlet is connected to the sheath liquid inlet and the cleaning device.

Description

Technical Field

[0001] The invention belongs to detection technology, and particularly refers to a flow fluorescence detection liquid path system. Background Art

[0002] Flow fluorescence detection, also known as suspension array, liquid chip, etc. The principle of this technology is based on fluorescently encoded microspheres. The microneedle molecules on the fluorescently encoded microspheres can specifically bind to biological molecules to achieve the effect of fluorescently labeling biological molecules. Laser analysis is performed on the fluorescently labeled biological molecules, and then a series of processing is performed on the light signals generated by the laser analysis to obtain visual diagnostic results.

[0003] The existing Chinese utility model with announcement number "CN205209962U" discloses a liquid path system of a flow fluorescence detector, which utilizes the above-mentioned fluorescence detection technology, including a sample, a sheath fluid, a swab, a sample injection pump, a sheath fluid injection pump, a peristaltic pump and a flow cell, wherein a first solenoid valve and a second solenoid valve are provided between the flow cell and the sample injection pump, a third solenoid valve is provided between the sample injection pump and the sheath fluid, a fourth solenoid valve is provided at one end of the sheath fluid injection pump, and a fifth solenoid valve is provided at the other end, the fourth solenoid valve is connected to the sheath fluid, the fifth solenoid valve is connected to the flow cell, the flow cell is connected to the waste liquid outlet through a sixth solenoid valve, the swab is connected to the peristaltic pump through a seventh solenoid valve, and the peristaltic pump is provided between the fourth solenoid valve and the fifth solenoid valve. After each sample is detected or before another sample is detected, the flow cell and the swab need to be cleaned with sheath fluid to avoid residual samples reducing the accuracy of the detection.

[0004] However, the existing technology is not perfect. If the sheath fluid needs to be used to clean the flow cell through a peristaltic pump, the internal resistance of the peristaltic pump is relatively high, resulting in a problem of low cleaning efficiency. Invention content

[0005] In order to overcome the deficiencies and existing problems of the prior art, the present invention provides a flow fluorescence detection liquid path system.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A flow type fluorescence detection liquid path system comprises a flow chamber, wherein the flow chamber has a sheath liquid inlet, a sample inlet and a waste liquid outlet, wherein the sheath liquid inlet is connected to a pulsation damper through a fifth control valve, the sample inlet is connected to a sampling device through a seventh control valve, the waste liquid outlet is connected to a waste liquid bottle, a cleaning device is provided on the sampling device, and further comprises a first control valve, wherein the first control valve has a first inlet and two first outlets, one first outlet is connected to the pulsation damper, and the other first outlet is connected to the sheath liquid inlet and the cleaning device.

[0008] In some embodiments, a sixth control valve is further included, wherein the sixth control valve has a sixth inlet and two sixth outlets, a first outlet is connected to the sixth inlet, a sixth outlet is connected to the sheath liquid inlet, and another sixth outlet is connected to the cleaning device.

[0009] In some embodiments, a second three-way pipe is further included, wherein a first end of the second three-way pipe is connected to a sixth outlet, a second end of the second three-way pipe is connected to a sheath liquid inlet, and a third end of the second three-way pipe is connected to a waste liquid bottle via a fourth control valve.

[0010] In some embodiments, the waste liquid bottle is connected to a waste liquid pump.

[0011] In some embodiments, an eighth control valve is further included, the eighth control valve has an eighth inlet and two eighth outlets, the eighth inlet is connected to the waste liquid pump, one eighth outlet is connected to the cleaning device, and the other eighth outlet is connected to the waste liquid bottle.

[0012] In some embodiments, a third control valve is further included, and the waste liquid bottle is connected to the waste liquid outlet via the third control valve.

[0013] In some embodiments, the device further includes a filter, wherein a first outlet is connected to the pulsation damper through the filter.

[0014] In some embodiments, it also includes a first three-way pipe, the first end of the first three-way pipe is connected to the filter, the second end of the first three-way pipe is connected to the pulsation damper, the third end of the first three-way pipe is connected to the second control valve, and the second control valve is connected to the waste liquid bottle.

[0015] In some embodiments, there are two sheath fluid inlets, a pulsation damper is connected to one sheath fluid inlet, and a first outlet is connected to the other sheath fluid inlet.

[0016] In some embodiments, the first inlet is connected to a sheath fluid pump, the seventh control valve has a seventh inlet and two seventh outlets, the seventh inlet is connected to a sample pump, the sample inlet is connected to one of the seventh outlets, and the sampling device is connected to another seventh outlet.

[0017] Compared with the prior art, the present invention has the following outstanding and beneficial technical effects:

[0018] (1) In the invention, various valves are used to realize the on-off and flow direction adjustment of the medium in the pipeline, and realize the functions of detection, zone cleaning, pressure relief and discharge of air in the pipeline. The parts are relatively simple and universal. Therefore, the invention has the advantages of simple structure, complete functions and low cost.

[0019] (2) In the present invention, when the flow fluorescence detection liquid path system is used to detect samples, the first control valve controls the sheath liquid to flow to the pulsation damper, and the sheath liquid flows to the sheath liquid inlet through the pulsation damper. The pulsation damper improves the medium stability of the sheath liquid. When the flow fluorescence detection liquid path system is used for cleaning, the first control valve can also control the medium to flow directly to the sheath liquid inlet and the cleaning device to avoid the pulsation damper hindering the flow of the medium. Therefore, the present invention has the advantage of high cleaning efficiency.

[0020] (3) In the invention, the medium can be cleaned in sequence through the second control valve, the sixth control valve, the flow chamber, the third control valve, the waste liquid bottle, the eighth control valve and the waste liquid pump. The medium can also be cleaned in sequence through the second control valve, the sixth control valve, the cleaning device, the eighth control valve and the waste liquid pump, thereby realizing diversion cleaning of the flow chamber and the cleaning device and improving the cleaning effect.

[0021] (4) In the present invention, the medium between the first control valve and the pulsation damper contains not only sheath fluid but also air. In order to improve the accuracy of detection, the air between the first control valve and the pulsation damper needs to be discharged before normal detection can be performed. If the second control valve is opened, the air between the first control valve and the pulsation damper can be quickly discharged to the waste liquid bottle. If the second control valve is closed, the sheath fluid can flow into the pulsation damper normally. In addition, when the detection is completed, the second control valve can be opened to release the medium between the first control valve and the pulsation damper to the waste liquid bottle to avoid the above-mentioned medium from accumulating pressure on the pulsation damper. Therefore, the present invention has the advantages of high working efficiency and reliable use. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the invention;

[0023] In the figure: 1-flow chamber, 2-sampling device, 3-pulsation damper, 4-waste liquid bottle, 5-cleaning device, 6-filter, 71-first control valve, 72-second control valve, 73-third control valve, 74-fourth control valve, 75-fifth control valve, 76-sixth control valve, 77-seventh control valve, 78-eighth control valve, 81-first three-way pipe, 82-second three-way pipe, 91-waste liquid pump, 92-sheath liquid pump, 93-sample pump, 11-sheath liquid inlet, 12-sample inlet, 13-waste liquid outlet, 711-first inlet, 712-first outlet, 761-sixth inlet, 762-sixth outlet, 781-eighth inlet, 782-eighth outlet. DETAILED DESCRIPTION

[0024] In order to facilitate understanding by those skilled in the art, the present invention is further described below in conjunction with the accompanying drawings and specific embodiments.

[0025] It should be noted that if a component is referred to as being “disposed on” another component, it may be directly on the other component or an intermediate component may be present between the two. If a component is considered to be “connected to” another component, it may be directly connected to the other component or an intermediate component may be present between the two.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art to which the invention relates. The terms used herein are for the purpose of describing specific embodiments only and are not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the related listed items.

[0027] like Figure 1 As shown, an embodiment of the present invention provides a flow fluorescence detection liquid path system, including a flow chamber 1, a pulsation damper 3, a seventh control valve 77, a sampling device 2, a cleaning device 5, and a first control valve 71.

[0028] The flow cell 1 has a sheath fluid inlet 11 , a sample inlet 12 , and a waste fluid outlet 13 .

[0029] The sheath liquid inlet 11 refers to an opening for the sheath liquid to flow into the flow chamber 1. The sample inlet 12 refers to an opening for the sample to flow into the flow chamber 1. The waste liquid outlet 13 refers to an opening for the waste liquid after detection to flow out of the flow chamber 1.

[0030] The sheath fluid inlet 11 is connected to the pulsation damper 3 via the fifth control valve 75 .

[0031] The device further includes a fifth control valve 75 , through which the pulsation damper 3 is connected to the sheath fluid inlet 11 .

[0032] The pulsation damper 3 refers to a pressure vessel for eliminating sheath fluid pressure pulsation, which stabilizes the sheath fluid pressure and flow and protects downstream equipment. The sheath fluid can flow to the sheath fluid inlet 11 through the pulsation damper 3 and the fifth control valve 75 .

[0033] The fifth control valve 75 is used to control the connection between the pulsation damper 3 and the sheath liquid inlet 11. If the fifth control valve 75 is opened, the pulsation damper 3 is connected to the sheath liquid inlet 11. If the fifth control valve 75 is closed, the connection between the pulsation damper 3 and the sheath liquid inlet 11 is blocked.

[0034] In actual use, if the flow fluorescence detection liquid path system is used to detect samples, the fifth control valve 75 is opened; if the flow fluorescence detection liquid path system is used for cleaning, the fifth control valve 75 is closed to prevent the sheath fluid in the flow chamber 1 from flowing back into the pulsation damper 3.

[0035] Specifically, the fifth control valve 75 is a solenoid valve, and the opening and closing of the fifth control valve 75 can be automatically controlled by a controller to achieve automated control.

[0036] The sample inlet 12 is connected to the sampling device 2 via a seventh control valve 77 .

[0037] The sampling device 2 is used to collect samples. The sample refers to blood, body fluids and tissue fluids of organisms. The sample collected by the sampling device 2 is indirectly delivered to the sample inlet 12 through the seventh control valve 77, and the seventh control valve 77 is used to control whether the sample flows to the sample inlet 12.

[0038] Specifically, the sampling device 2 is a sampling needle. The sampling needle refers to a tube body with a needle-shaped overall structure. When the sampling needle is inserted into the sample, the sampling needle can suck the sample into the sampling needle under the action of negative pressure.

[0039] The waste liquid outlet 13 is connected to the waste liquid bottle 4 .

[0040] The waste liquid bottle 4 is hollow and is used to temporarily store waste liquid, which refers to a mixed liquid of sheath liquid, sample, etc.

[0041] The sampling device 2 is provided with a cleaning device 5 .

[0042] The cleaning device 5 is used to clean the sampling device 2 to prevent the sample remaining on the sampling device 2 from contaminating the sample collected next time.

[0043] Specifically, the cleaning device 5 is a swab, which is sleeved on the sampling device 2. If sheath fluid flows on the swab, the sheath fluid can flow through the surface of the sampling device 2, thereby cleaning the sampling device 2. The specific structure of the swab can refer to a swab for cleaning a sampling needle in the announcement number "CN208019077U".

[0044] The first control valve 71 is also included. The first control valve 71 has a first inlet 711 and two first outlets 712 . One first outlet 712 is connected to the pulsation damper 3 , and the other first outlet 712 is connected to the sheath liquid inlet 11 and the cleaning device 5 .

[0045] The first control valve 71 can change the flow direction of the medium. If one first outlet 712 is open and the other first outlet 712 is closed, the medium flowing into the first inlet 711 can flow to the pulsation damper 3 through the first outlet 712. If one first outlet 712 is closed and the other first outlet 712 is open, the medium flowing into the first inlet 711 can flow to the sheath liquid inlet 11 and the cleaning device 5 through the other first outlet 712. In actual use, the above-mentioned medium can be a sheath liquid, which can be used not only for detection but also for cleaning.

[0046] Specifically, the first control valve 71 is an electromagnetic three-way valve, and the flow direction of the sheath liquid of the first control valve 71 can be automatically controlled by a controller to achieve automatic control.

[0047] In the present invention, when the flow fluorescence detection liquid circuit system is used to detect samples, the first control valve 71 controls the sheath liquid to flow to the pulsation damper 3, and the sheath liquid flows to the sheath liquid inlet 11 through the pulsation damper 3. The pulsation damper 3 improves the medium stability of the sheath liquid; if the flow fluorescence detection liquid circuit system is used for cleaning, the first control valve 71 can also control the medium to flow directly to the sheath liquid inlet 11 and the cleaning device 5 to avoid the pulsation damper 3 hindering the flow of the medium. Therefore, the present invention has the advantages of simple structure, high cleaning efficiency and accurate detection.

[0048] It also includes a sixth control valve 76 , which has a sixth inlet 761 and two sixth outlets 762 , a first outlet 712 connected to the sixth inlet 761 , a sixth outlet 762 connected to the sheath liquid inlet 11 , and another sixth outlet 762 connected to the cleaning device 5 .

[0049] The sixth control valve 76 can change the flow direction of the sheath liquid. If one sixth outlet 762 is open and the other sixth outlet 762 is closed, the sheath liquid flowing into the sixth inlet 761 can flow to the sheath liquid inlet 11 through the one sixth outlet 762. If one sixth outlet 762 is closed and the other sixth outlet 762 is open, the sheath liquid flowing into the sixth inlet 761 can clean the device 5 through the other sixth outlet 762. Therefore, the split flow flushing of the cleaning device 5 and the flow chamber 1 is achieved.

[0050] Specifically, the sixth control valve 76 is an electromagnetic three-way valve, and the flow direction of the sheath liquid of the sixth control valve 76 can be automatically controlled by the controller to achieve automatic control.

[0051] It also includes a second three-way pipe 82, a first end of the second three-way pipe 82 is connected to a sixth outlet 762, a second end of the second three-way pipe 82 is connected to the sheath liquid inlet 11, and a third end of the second three-way pipe 82 is connected to the waste liquid bottle 4 through a fourth control valve 74.

[0052] The second three-way pipe 82 is a pipe body with three openings. If the fourth control valve 74 is open, the sheath liquid flowing into the second three-way pipe 82 can flow to the waste liquid bottle 4 through the fourth control valve 74. If the fourth control valve 74 is closed, the sheath liquid flowing into the second three-way pipe 82 can only flow out through the sheath liquid inlet 11.

[0053] Specifically, the fourth control valve 74 is a solenoid valve, and the opening and closing of the fourth control valve 74 can be automatically controlled by a controller to achieve automated control.

[0054] The waste liquid bottle 4 is connected to a waste liquid pump 91 .

[0055] The waste liquid pump 91 is used to drive the waste liquid in the waste liquid bottle 4 to flow toward the waste liquid pump 91 and be discharged.

[0056] It also includes an eighth control valve 78, an eighth inlet 781 and two eighth outlets 782 of the eighth control valve 78, the eighth inlet 781 is connected to the waste liquid pump 91, one eighth outlet 782 is connected to the cleaning device 5, and the other eighth outlet 782 is connected to the waste liquid bottle 4.

[0057] The eighth control valve 78 is used to control the flow direction of the medium. If one eighth outlet 782 is opened and another eighth outlet 782 is closed, the waste liquid pump 91 can drive the medium to flow on the cleaning device 5. If one eighth outlet 782 is closed and another eighth outlet 782 is opened, the waste liquid pump 91 can drive the medium to flow on the waste liquid bottle 4.

[0058] Specifically, the eighth control valve 78 is an electromagnetic three-way valve, and the flow direction of the sheath liquid of the eighth control valve 78 can be automatically controlled by the controller to achieve automatic control.

[0059] In actual use, the sheath liquid can be cleaned in sequence through the second control valve 72, the sixth control valve 76, the flow chamber 1, the third control valve 73, the waste liquid bottle 4, the eighth control valve 78 and the waste liquid pump 91. The sheath liquid can also be cleaned in sequence through the second control valve 72, the sixth control valve 76, the cleaning device 5, the eighth control valve 78 and the waste liquid pump 91, thereby realizing the diversion cleaning of the flow chamber 1 and the cleaning device 5, thereby improving the cleaning effect.

[0060] The waste liquid outlet 13 is connected to the waste liquid bottle 4 through the third control valve 73 .

[0061] The third control valve 73 is used to control the connection between the waste liquid outlet 13 and the waste liquid bottle 4. If the third control valve 73 is opened, the waste liquid outlet 13 is connected to the waste liquid bottle 4. If the third control valve 73 is disconnected, the connection between the waste liquid outlet 13 and the waste liquid bottle 4 is blocked.

[0062] Specifically, the third control valve 73 is a solenoid valve, and the opening and closing of the third control valve 73 can be automatically controlled by a controller to achieve automated control.

[0063] It also includes a filter 6 , and a first outlet 712 is connected to the pulsation damper 3 through the filter 6 .

[0064] The filter 6 is used to filter impurities in the sheath liquid flowing into the pulsation damper 3, so as to avoid the problem that the impurities in the sheath liquid enter the flow chamber 1 and cause the detection accuracy to decrease.

[0065] It also includes a first three-way pipe 81, a first end of the first three-way pipe 81 is connected to the filter 6, a second end of the first three-way pipe 81 is connected to the pulsation damper 3, a third end of the first three-way pipe 81 is connected to a second control valve 72, and the second control valve 72 is connected to the waste liquid bottle 4.

[0066] The first three-way pipe 81 is a pipe body with three openings. If the second control valve 72 is opened, the medium between the first control valve 71 and the pulsation damper 3 can flow into the waste liquid bottle 4 through the second control valve 72. If the second control valve 72 is closed, the medium between the first control valve 71 and the pulsation damper 3 can only flow out through the pulsation damper 3.

[0067] Specifically, the second control valve 72 is a solenoid valve, and the opening and closing of the second control valve 72 can be automatically controlled by a controller to achieve automated control.

[0068] In actual use, the medium between the first control valve 71 and the pulsation damper 3 contains not only sheath fluid but also air. In order to improve the accuracy of detection, the air between the first control valve 71 and the pulsation damper 3 needs to be discharged before normal detection can be performed. If the second control valve 72 is opened, the air between the first control valve 71 and the pulsation damper 3 can be quickly discharged to the waste liquid bottle 4. If the second control valve 72 is closed, the sheath fluid can flow into the pulsation damper 3 normally. In addition, when the detection is completed, the second control valve 72 can be opened to release the medium between the first control valve 71 and the pulsation damper 3 to the waste liquid bottle 4 to avoid the above-mentioned medium accumulating pressure on the pulsation damper 3. Therefore, the invention has the advantages of high working efficiency and reliable use.

[0069] There are two sheath liquid inlets 11 , a first outlet 712 is connected to one sheath liquid inlet 11 , and a fifth control valve 75 is connected to the other sheath liquid inlet 11 .

[0070] The two sheath liquid inlets 11 are independent of each other, and when the flow fluorescence detection liquid path system is in the process of cleaning or detection, interference occurs between them, thereby improving the working reliability of the invention.

[0071] Specifically, two sheath liquid inlets 11 are symmetrically arranged on both sides of the flow chamber 1 , and the two sheath liquid inlets 11 are connected to the inner cavity of the flow chamber 1 respectively.

[0072] The first inlet 711 is connected to a sheath liquid pump 92 .

[0073] The sheath liquid pump 92 is used to deliver the sheath liquid to the first inlet 711 and provides pressure for delivering the sheath liquid.

[0074] It also includes a sample pump 93 . The seventh control valve 77 has a seventh inlet 771 and two seventh outlets 772 . The sample pump 93 is connected to the seventh inlet 771 . The sample inlet is connected to one seventh outlet 772 . The sampling device 2 is connected to the other seventh outlet 772 .

[0075] The sample pump 93 is used to provide negative pressure to the sampling device 2, and the sample pump 93 is also used to provide positive pressure to the sampling inlet 12. The seventh control valve 77 is used to change the flow direction of the medium. If one seventh outlet 772 is opened and another seventh outlet 772 is closed, the sample on the sampling device 2 can flow into the sample pump 93. If one seventh outlet 772 is closed and another seventh outlet 772 is opened, the sample in the sample pump 93 can flow into the sample inlet 12.

[0076] Specifically, the seventh control valve 77 is an electromagnetic three-way valve, and the flow direction of the sample on the seventh control valve 77 can be automatically controlled by the controller to achieve automatic control.

[0077] The sample pump 93 is used to transport the sample on the sampling device 2 to the seventh control valve 77 .

[0078] The following describes the detection method of the flow fluorescence detection liquid system:

[0079] The sample pump 93 is turned on, and the seventh control valve 77 is controlled to connect the sample pump 93 and the sampling device 2 , so that the sample pump 93 collects samples through the sampling device 2 .

[0080] If the sampling device 2 collects a sufficient amount of sample, the sheath liquid pump 92 is turned on, the seventh control valve 77 is controlled to connect the sample pump 93 and the sample inlet 12, the first control valve 71 is controlled to connect the sheath liquid pump 92 and the filter 6, the second control valve 72 is closed, the third control valve 73 is opened, the fourth control valve 74 is closed, the fifth control valve 75 is opened, the sixth control valve 76 is controlled to block the connection between the first control valve 71 and the flow chamber 1, and the eighth control valve 78 is controlled to connect the waste liquid bottle 4 and the waste liquid pump 91. The sheath liquid passes through the sheath liquid pump 92, the first control valve 71, the filter 6, the pulsation damper 3, the fifth control valve 75, the flow chamber 1, the third control valve 73 and the waste liquid bottle 4 in sequence, and the sample passes through the sample pump 93, the seventh control valve 77, the flow chamber 1, the third control valve 73 and the waste liquid bottle 4 in sequence.

[0081] When cleaning the sampling device 2, the sheath liquid pump 92 and the waste liquid pump 91 are turned on, the first control valve 71 is controlled to connect the sheath liquid pump 92 and the sixth control valve 76, the sixth control valve 76 is controlled to connect the first control valve 71 and the cleaning device 5, and the eighth control valve 78 is controlled to connect the cleaning device 5 and the waste liquid pump 91. The sheath liquid can pass through the sheath liquid pump 92, the first control valve 71, the sixth control valve 76, the cleaning device 5, the eighth control valve 78 and the waste liquid pump 91 in sequence, thereby realizing the cleaning of the sampling device 2.

[0082] When cleaning the flow chamber 1, the sheath liquid pump 92 and the waste liquid pump 91 are turned on, the first control valve 71 is controlled to connect the sheath liquid pump 92 and the sixth control valve 76, the sixth control valve 76 is controlled to connect the first control valve 71 and the flow chamber 1, the fourth control valve 74 is closed, the third control valve 73 is opened, and the eighth control valve 78 is controlled to connect the waste liquid bottle 4 and the waste liquid pump 91. The sheath liquid can pass through the sheath liquid pump 92, the first control valve 71, the sixth control valve 76, the second three-way pipe 82, the flow chamber 1, the third control valve 73, the waste liquid bottle 4, the eighth control valve 78 and the waste liquid pump 91 in sequence, thereby achieving the cleaning of the flow chamber 1.

[0083] The above embodiments are only preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made to the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A flow type fluorescence detection fluid path system, comprising a flow chamber, the flow chamber having a sheath liquid inlet, a sample inlet and a waste liquid outlet, characterized in that: The sheath liquid inlet is connected to the pulsation damper through the fifth control valve, the sample inlet is connected to the sampling device through the seventh control valve, the waste liquid outlet is connected to the waste liquid bottle, the sampling device is provided with a cleaning device, and further includes a first control valve, the first control valve has a first inlet and two first outlets, one first outlet is connected to the pulsation damper, and the other first outlet is connected to the sheath liquid inlet and the cleaning device; Also includes a filter, a first outlet connected to the pulsation damper through the filter; It also includes a first three-way pipe, a first end of the first three-way pipe is connected to the filter, a second end of the first three-way pipe is connected to the pulsation damper, a third end of the first three-way pipe is connected to a second control valve, and the second control valve is connected to the waste liquid bottle; Also includes a sixth control valve, the sixth control valve has a sixth inlet and two sixth outlets, a first outlet is connected to the sixth inlet, a sixth outlet is connected to the sheath liquid inlet, and another sixth outlet is connected to the cleaning device; It also includes a second three-way pipe, wherein a first end of the second three-way pipe is connected to a sixth outlet, a second end of the second three-way pipe is connected to a sheath liquid inlet, and a third end of the second three-way pipe is connected to a waste liquid bottle through a fourth control valve; The first control valve controls the sheath liquid to flow to the pulsation damper, and the sheath liquid flows to the sheath liquid inlet through the pulsation damper; if the flow fluorescence detection liquid path system is used for cleaning, the first control valve can also control the medium to flow directly to the sheath liquid inlet and the cleaning device.

2. A flow fluorescence detection liquid path system according to claim 1, characterized in that: The waste liquid bottle is connected with a waste liquid pump.

3. A flow fluorescence detection liquid path system according to claim 2, characterized in that: It also includes an eighth control valve, which has an eighth inlet and two eighth outlets. The eighth inlet is connected to the waste liquid pump, one eighth outlet is connected to the cleaning device, and the other eighth outlet is connected to the waste liquid bottle.

4. A flow fluorescence detection liquid path system according to claim 1, 2 or 3, characterized in that: It also includes a third control valve, and the waste liquid bottle is connected to the waste liquid outlet through the third control valve.

5. A flow fluorescence detection liquid path system according to claim 1, characterized in that: There are two sheath liquid inlets, a pulsation damper is connected to one sheath liquid inlet, and a first outlet is connected to the other sheath liquid inlet.

6. A flow fluorescence detection liquid path system according to claim 1, characterized in that: The first inlet is connected to a sheath liquid pump, the seventh control valve has a seventh inlet and two seventh outlets, the seventh inlet is connected to a sample pump, the sample inlet is connected to one seventh outlet, and the sampling device is connected to another seventh outlet.

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