A fully automatic platelet antibody detection analyzer and its use method

Abstract

The present invention relates to a fully automatic platelet antibody detection analyzer and a method for using the same. The analyzer comprises a control system, a circuit board, a base, a pipette tip storage module, a sample storage module, a gel card storage puncture module, a test waste discard module, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module and a sample loading arm slide rail module. The method for using the analyzer comprises the following steps: placing a pipette tip, a platelet suspension, a diluent, a fluorescent reagent, a sample tube and a gel card into corresponding positions, and setting detection sample parameters; puncturing a sealing film of the gel card; adding the platelet suspension, the diluent, the fluorescent reagent and a plasma sample into the gel card; after incubating and centrifuging the gel card, taking photos for interpretation and detecting the fluorescence intensity, and providing qualitative and quantitative detection results. The gel card is discarded after the detection is completed, and this process is repeated until all the detections are completed. The present invention is helpful for clinical diagnosis of platelet immune diseases and improving the efficacy of platelet transfusion.

Description

Technical Field

[0001] The present invention relates to the field of medical devices, and in particular to a fully automatic platelet antibody detection analyzer and a use method thereof. Background Art

[0002] Platelet antigens can stimulate the body to produce platelet antibodies through blood transfusion, pregnancy, organ transplantation and other ways, causing platelet destruction and immune thrombocytopenia, such as platelet transfusion refractoriness (PTR), fetomaternal alloimmunethrombocytopenia (FMAIT) and post-transfusion purpura (PTP). In recent years, platelet transfusion has been widely used in the treatment of patients with clinical thrombocytopenia, such as autoimmune thrombocytopenia, neonatal thrombocytopenia, aplastic anemia, tumors and other patients. At present, there is no such instrument as fully automatic platelet antibody detection analyzer on the market, and the platelet antibody detection method commonly used in clinical practice usually requires manual participation for operation and interpretation, and its detection method is usually MAIPA method and flow cytometry, wherein the MAIPA method is a manual operation for testing, and platelets need to be lysed during the test process. The method has cumbersome operation steps and is time-consuming (more than 5 hours), which is difficult to meet the needs of clinical routine testing. Flow cytometry requires expensive flow cytometry equipment, and repeated washing is required during the detection process, which is time-consuming and laborious, and the detection flux is low, which cannot meet the needs of clinical testing. At present, there is still a lack of effective and practical platelet antibody detection technology in clinical practice. The frequent occurrence of invalid platelet transfusion events caused by platelet antibodies not only delays patient treatment, but also causes a waste of precious platelet resources. Summary of the invention

[0003] The purpose of the present invention is to provide a fully automatic platelet antibody detection analyzer, which is helpful for clinical diagnosis of platelet immune diseases and can also perform blood type compatibility detection before platelet transfusion, greatly improving the transfusion efficacy.

[0004] In order to achieve the above objectives, the following technical solutions are provided:

[0005] A fully automatic platelet antibody detection analyzer and a method of using the same, the analyzer comprising a control system, a circuit board, a base, a pipette tip storage module, a sample storage module, a gel card storage puncture module, a test waste discard module, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module and a sample loading arm slide rail module, the control system controls the operation of the circuit board, the circuit board controls the actions of each module, the pipette tip storage module, the sample storage module, the gel card storage puncture module, the test waste discard module, the centrifugal incubation module, the reagent storage module, the card clamping manipulator module, the detection module and the beam module are all fixedly connected to the base, along which the sample loading arm slide rail module is mounted. The base is provided with a tip storage module, a sample storage module, a gel card storage puncture module, a test waste discard module and a centrifugal incubation module from left to right in the length direction. The reagent storage module is located at the rear side of the tip storage module, the circuit board is located at the rear side of the reagent storage module, the card clamping manipulator module is located at the rear side of the test waste discard module, and the detection module is located at the rear side of the centrifugal incubation module. The beam module includes a sliding bracket and a synchronous bracket. The length direction of the sliding bracket and the synchronous bracket is parallel to the length direction of the base, and they are symmetrically arranged at the front and rear ends of the base respectively. The front end of the sample loading arm slide rail module is slidably connected to the sliding bracket, and the rear end thereof is installed on the synchronous bracket.

[0006] Preferably, the tip storage module is a drawer-type push-pull structure, which has two tip boxes arranged side by side in front and behind, and a certain number of tips are arranged in the tip boxes;

[0007] The sample storage module is provided with six rows of sample racks arranged side by side on the left and right, each row of sample racks can accommodate twelve sample tubes, and each row of sample racks is provided with an independent slideway;

[0008] The gel card storage puncture module includes a gel card holder, a track, a screw rod, a positioning motor, a slider, and a puncture mechanism. A certain number of gel card positions are arranged side by side on the top of the gel card holder, and a slider is fixedly connected to the bottom. The bottom surface of the slider abuts against the top surface of the track and can slide relatively. The positioning motor is fixedly connected to one end of the track, and its output end is fixedly connected to one end of the screw rod. The screw rod passes through the slider and is threadedly connected to it, and the other end of the screw rod is rotatably connected to the other end of the track. The puncture mechanism is located above the middle of the track and includes a puncture bracket, a puncture motor, an eccentric wheel, A puncturing rail, a puncturing slider, a tension spring and a knife seat, wherein the bottom end of the puncturing bracket is fixedly connected to the base, the puncturing motor is fixedly connected to the upper part of the puncturing bracket, and an eccentric wheel is fixedly connected to the output end thereof, there are two puncturing rails, which are symmetrically fixedly connected to the middle part of the puncturing bracket, and each of the puncturing rails is slidably connected to a puncturing slider, the knife seat is fixedly connected to the two puncturing sliders, and a certain number of puncturing knives are arranged on the knife seat, there are two tension springs, which are respectively located in front of the two puncturing rails, one end of the tension spring is fixedly connected to the puncturing bracket, and the other end of the tension spring is fixedly connected to the knife seat;

[0009] The test waste discarding module is a drawer-type push-pull structure, and three sets of light pipes are arranged on both sides to facilitate monitoring whether the test waste discarding module is filled;

[0010] The centrifugal incubation module includes a centrifuge and a temperature control module, the temperature control module includes a heating belt and a temperature sensor, the heating belt is arranged on the bottom surface of the centrifuge, and the temperature sensor is arranged on the top of the side of the centrifuge;

[0011] The reagent storage module is provided with three reagent positions side by side on the left and right;

[0012] The clamping robot module is provided with a clamping module mounting seat, a robot telescopic component, a robot mounting seat, a finger component, a robot rotating component and a rubber sleeve in sequence from bottom to top. The robot telescopic component includes a telescopic belt, a telescopic motor, a fourth pulley group and a lifting block. One side of the telescopic motor is horizontally fixedly connected to the bottom of the outer side of the clamping module mounting seat, and its output end passes through the clamping module mounting seat. The fourth pulley group includes a fourth active pulley and a fourth driven pulley. The fourth active pulley is fixedly connected to the output end of the telescopic motor, and the fourth driven pulley is installed on the top of the inner side of the clamping module mounting seat. The inner side of the telescopic belt is cooperatively connected with the fourth pulley group, and the lifting block is connected to the telescopic belt. One side of the belt is fixedly connected, the manipulator rotating component includes a rotating rod, a rotating belt, a rotating motor and a third pulley group, one side of the rotating motor is vertically fixedly connected to the upper part of the clamping module mounting seat, and its output end passes through the clamping module mounting seat, the two ends of the rotating rod are respectively rotatably connected to the clamping module mounting seat, the third driven pulley group includes a third active pulley and a third driven pulley, the third active pulley is fixedly connected to the output end of the rotating motor, the third driven wheel is fixedly connected to the top of the rotating rod, one end of the manipulator mounting seat is fixedly sleeved with a rubber sleeve, and the other end top surface is fixedly connected with a finger component, the rotating rod passes through the rubber sleeve and is slidably and frictionally connected thereto;

[0013] The detection module includes a fixed plate, a constant current source, a detection module mounting frame, a light source, an optical detection module, an optical darkroom, a camera, a camera fixing seat, a light source slide rail and a backlight source. The constant current source, the detection module mounting frame, the camera fixing seat, the light source slide rail and the backlight source are all fixedly connected to the fixed plate, the light source is electrically connected to the constant current source, the optical darkroom is fixedly connected to the light source slide rail, the optical detection module is fixedly connected to the detection module mounting frame, and the camera is fixedly connected to a side surface of the camera fixing seat;

[0014] The synchronous bracket is also provided with a synchronous belt, a first pulley group, a synchronous motor, a synchronous slide rail and a slide table. The bottom end of the synchronous motor is fixedly connected to the upper right end of the synchronous bracket. The first pulley group includes a first active pulley and a first driven pulley. The first active pulley is fixedly connected to the output end of the synchronous motor. The first driven pulley is installed on the left top end of the synchronous bracket. The inner side of the synchronous belt is cooperatively connected with the first pulley group. The synchronous slide rail is arranged at the rear side of the synchronous belt. The slide table is slidably connected with the synchronous slide rail, and one side of the slide table is fixedly connected with the synchronous belt.

[0015] The loading arm slide module includes a loading arm slide and three electronic pipette guns arranged side by side in front and behind. The front end bottom of the loading arm slide is slidably connected to the sliding bracket, and the rear end bottom surface is fixedly connected to the top surface of the slide. The upper inner side of the loading arm slide is also provided with three groups of transmission mechanisms arranged side by side up and down. Taking one of the groups as an example, the transmission mechanism includes a second pulley group, a slide plate, a transmission motor and a transmission belt. The second pulley group includes a second active pulley and a second driven pulley. The second driven pulley is installed on the inner front end of the loading arm slide. One side of the transmission motor is fixedly connected to the inner rear end of the loading arm slide, and the output end of the transmission motor is fixedly connected to the second active pulley. The inner side of the transmission belt is cooperatively connected with the second pulley group. One side of the slide plate is slidably connected to the loading arm slide, which passes through the transmission belt and is fixedly connected to one side of the transmission belt. The other side of the slide plate is fixedly connected to the electronic pipette gun.

[0016] Preferably, the method of use comprises the following steps:

[0017] (1) Before testing, the operator places the pipette tip into the pipette tip box (the system automatically records the remaining pipette tip amount from the last test), places the platelet suspension, diluent, and fluorescent reagent into the three reagent positions of the reagent storage module from left to right, places the sample tube into the sample rack, and places the corresponding number of gel cards (each gel card is for six people) in the gel card storage area according to the number of sample tubes to be tested;

[0018] (2) Use the control system to set parameters such as the number of test samples, incubation temperature, incubation time, centrifugation speed, and centrifugation time (parameters can be named and saved);

[0019] (3) The control system performs the following operation instructions according to the number of samples:

[0020] ①Transfer the gel card to the gel card storage puncture module to puncture the sealing film;

[0021] ② Use the sample loading arm slide module to add platelet suspension, diluent, fluorescent reagent and patient plasma sample to the gel card in sequence;

[0022] ③ Use the card clamping robot module to grab the gel card from the gel card storage area and put it into the centrifugal incubation module; repeat the clamping action to put all the gel cards with added samples into the centrifugal incubation module;

[0023] ④After constant temperature incubation and centrifugation, the gel card is placed into the detection module using the card clamping robot module;

[0024] ⑤ After the gel card enters the detection module, it is first photographed and interpreted, and then transferred to the optical darkroom through the light source slide rail for fluorescence intensity detection, and the qualitative and quantitative detection results are given through the image processing algorithm and A / D amplification algorithm;

[0025] ⑥ After a gel card test is completed, use the card clamping robot module to grab the gel card, turn it to the test waste disposal module for disposal, and then use the card clamping robot module to grab the gel card from the centrifugal incubation module and put it into the detection module for detection, and repeat this process until all tests are completed.

[0026] The beneficial effects of the present invention are:

[0027] The present invention is used in conjunction with a gel card to replace manual operations and automatically complete a series of processes such as sample addition, incubation, centrifugation, photo interpretation, and fluorescence intensity analysis. It can detect platelet antibodies and perform blood type compatibility testing before platelet transfusion, reduce the occurrence of ineffective platelet transfusion events, and contribute to the clinical diagnosis of platelet immune diseases and improve the efficacy of platelet transfusion. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a structural schematic diagram of the present invention;

[0029] Figure 2 It is a structural schematic diagram of the tip storage module;

[0030] Figure 3 It is a structural diagram of a sample storage module;

[0031] Figure 4 This is a schematic diagram of the structure of the gel card storage puncture module;

[0032] Figure 5 It is a structural schematic diagram of the card clamping robot module;

[0033] Figure 6 It is a structural diagram of the detection module;

[0034] Figure 7 It is a structural schematic diagram of the beam module;

[0035] Figure 8 for Figure 7 A top view of

[0036] The reference numerals shown in the figure are: 1-pipette tip storage module, 2-sample rack, 3-gel card position, 4-experimental waste discarding module, 5-centrifuge, 6-reagent position, 7-puncture knife, 8-retractable belt, 9-optical darkroom, 10-beam module, 11-sample arm slide rail, 12-electronic pipette gun, 13-rotating belt, 14-clamping module mounting seat, 15-finger component, 16-manipulator mounting seat, 17-fixed plate, 18-constant current source, 19-detection module mounting bracket, 20-light source, 21-optical detection module, 22-backlight source, 23-gel card, 24-camera, 25-camera fixing seat, 26-light source slide rail, 27-sliding bracket, 28-synchronous bracket, 29-base, 30-synchronous belt, 31-first active pulley, 32-synchronous motor, 33-synchronous slide rail, 34-synchronous motor, 35-synchronous slide rail, 36-synchronous motor, 37-synchronous motor, 38-synchronous slide rail, 39-synchronous motor, 40-synchronous belt, 41-first active pulley, 42-synchronous motor, 43-synchronous slide rail, 44-synchronous motor, 45-synchronous slide rail, 46-synchronous motor, 47-synchronous motor, 48-synchronous motor, 49-synchronous motor, 50-synchronous belt, 51-first active pulley, 52-synchronous motor, 53-synchronous slide rail, 54-synchronous motor, 55-synchronous motor, 56-synchronous motor, 57-synchronous motor, 58-synchronous motor, 59-synchronous motor, 60-synchronous motor 4-slide, 35-circuit board, 36-light tube, 37-pipette box, 38-pipette, 39-sample tube, 40-slide, 41-track, 42-screw, 43-positioning motor, 44-slider, 45-temperature sensor, 46-heating belt, 47-second driven pulley, 48-slide plate, 49-transmission motor, 50-transmission belt, 51-puncture bracket, 52-puncture motor, 53-eccentric wheel, 54-puncture slide rail, 55-puncture slide, 56-tension spring, 57-gel card holder, 58-rubber sleeve, 59-rotating rod, 60-first driven pulley, 61-second active pulley, 62-knife holder, 63-telescopic motor, 64-fourth active pulley, 65-fourth driven pulley, 66-lifting block, 67-third active pulley, 68-third driven pulley, 69-rotating motor. DETAILED DESCRIPTION

[0037] The specific implementation modes of the present invention are described in detail below in conjunction with the accompanying drawings.

[0038] like Figures 1 to 7As shown, a fully automatic platelet antibody detection analyzer includes a control system, a circuit board 35, a base 29, a pipette tip storage module 1, a sample storage module, a gel card storage puncture module, a test waste discard module 4, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module 10 and a sample loading arm slide rail module. The control system controls the operation of the circuit board 35, and the circuit board 35 controls the actions of each module. The pipette tip storage module 1, the sample storage module, the gel card storage puncture module, the test waste discard module 4, the centrifugal incubation module, the reagent storage module, the card clamping manipulator module, the detection module and the beam module 10 are all fixedly connected to the base 29. The pipette tip storage module 1, the sample storage module, the gel card storage puncture module, the test waste discard module 4 and the centrifugal incubation module are arranged in sequence from left to right along the length direction of the base 29. The reagent storage module is located at the pipette tip storage module 1. On the rear side, the circuit board 35 is located at the rear side of the reagent storage module 1, the clamping manipulator module is located at the rear side of the test waste discarding module 4, and the detection module is located at the rear side of the centrifugal incubation module. The crossbeam module 10 is used to support and drive the sample loading slide rail module to move, including a sliding bracket 27 and a synchronous bracket 28. The length direction of the sliding bracket 27 and the synchronous bracket 28 is parallel to the length direction of the base 29, and they are symmetrically arranged at the front and rear ends of the base 29 respectively. The sample loading arm slide rail module is used to support and drive the electronic pipette gun 12 to move, and its front end is slidably connected to the sliding bracket 27, and its rear end is installed on the synchronous bracket 28; the tip storage module 1 is a drawer-type push-pull structure, and two tip boxes 37 are arranged side by side in front and back, and a certain number of tips 38 are arranged in the tip box 37; the sample storage module is provided with six rows of sample racks 2 side by side on the left and right, and each row of sample racks 2 can accommodate twelve sample tubes 39, and each row of sample racks 2 is equipped with an independent slide 40;The gel card storage puncture module includes a gel card holder 57, a track 41, a screw 42, a positioning motor 43, a slider 44, and a puncture mechanism. A certain number of gel card positions 3 are arranged side by side on the top of the gel card holder 57 for storing gel cards 23. A slider 44 is fixedly connected to the bottom thereof. The bottom surface of the slider 44 abuts against the top surface of the track 41 and can slide relatively. The positioning motor 43 is fixedly connected to one end of the track 41, and its output end is fixedly connected to one end of the screw 42. The screw 42 passes through the slider 44 and The screw rod 42 is threadedly connected, and the other end of the screw rod 42 is rotatably connected to the other end of the track 41. The puncturing mechanism is used to puncture the gel card 23, and is located above the middle of the track 41. It includes a puncturing bracket 51, a puncturing motor 52, an eccentric wheel 53, a puncturing slide rail 54, a puncturing slider 55, a tension spring 56 and a knife seat 62. The bottom end of the puncturing bracket 51 is fixedly connected to the base 29, the puncturing motor 52 is fixedly connected to the upper part of the puncturing bracket 51, and its output end is fixedly connected to an eccentric wheel 53. There are two puncturing slide rails 54, which are respectively connected to the puncturing bracket 51 and the puncturing slider 55. The puncture bracket 51 is fixedly connected to the middle part of the puncture bracket 51, and each puncture slide rail 54 is slidably connected to a puncture slider 55, and the knife holder 62 is fixedly connected to the two puncture sliders 55. A certain number of puncture knives 7 are arranged on the knife holder 62. There are two tension springs 56, which are respectively located in front of the two puncture slide rails 54. One end of the tension spring 56 is fixedly connected to the puncture bracket 51, and the other end is fixedly connected to the knife holder 62. The test waste discarding module 4 is a drawer-type push-pull structure for holding the discarded gel card 23 after the test, and three drawers are arranged on both sides. The light pipe 36 is assembled to monitor whether the test waste discarding module 4 is filled; the centrifugal incubation module is used to incubate and centrifuge the sample in the gel card 23 at a constant temperature, including a centrifuge 5 and a temperature control module. The temperature control module includes a heating belt 45 and a temperature sensor 45, which can monitor the temperature in the centrifuge 5 in real time, so as to better the effect of the gel card 23. The heating belt 46 is arranged on the bottom surface of the centrifuge 5, and the temperature sensor 45 is arranged on the top of the side of the centrifuge 5; the reagent storage module is provided with three reagent positions 6 side by side on the left and right;The card clamping robot module is used for moving and clamping the gel card 23, and is provided with a card clamping module mounting seat 14, a robot telescopic component, a robot mounting seat 16, a finger component 15, a robot rotating component and a rubber sleeve 58 from bottom to top. The robot telescopic component includes a telescopic belt 8, a telescopic motor 63, a fourth pulley group and a lifting block 66. One side of the telescopic motor 63 is horizontally fixedly connected to the bottom of the outer side of the card clamping module mounting seat 14, and its output end passes through the card clamping module mounting seat 14. The fourth pulley group includes a fourth active pulley 64 and a fourth driven pulley 65. The fourth active pulley 64 is fixedly connected to the output end of the telescopic motor 63, and the fourth driven pulley 65 is installed at the top of the inner side of the card clamping module mounting seat 14. The inner side of the telescopic belt 8 is connected to the outer side of the card clamping module mounting seat 14. The fourth pulley group is connected in cooperation, the lifting block 66 is fixedly connected to one side of the telescopic belt 8, the manipulator rotating component includes a rotating rod 59, a rotating belt 13, a rotating motor 69 and a third pulley group, one side of the rotating motor 69 is vertically fixedly connected to the upper part of the clamping module mounting seat 14, and its output end passes through the clamping module mounting seat 14, the two ends of the rotating rod 59 are respectively rotatably connected to the clamping module mounting seat 14, the third driven pulley group includes a third active pulley 67 and a third driven pulley 68, the third active pulley 67 is fixedly connected to the output end of the rotating motor 69, the third driven wheel 68 is fixedly connected to the top of the rotating rod 59, one end of the manipulator mounting seat 16 is fixedly sleeved with a rubber sleeve 58, and the other end of the top surface is fixedly connected There is a finger component 15 for clamping the gel card 23, and the rotating rod 59 passes through the rubber sleeve 58 and is connected with it by sliding friction; the detection module is used to take pictures and interpret the sample in the gel card 23 and detect the fluorescence intensity, including a fixed plate 17, a constant current source 18, a detection module mounting frame 19, a light source 20, an optical detection module 21, an optical darkroom 9, a camera 24, a camera fixing seat 25, a light source slide rail 26 and a backlight source 22. The constant current source 18, the detection module mounting frame 19, the camera fixing seat 25, the light source slide rail 26 and the backlight source 22 are all fixedly connected to the fixed plate 17, the light source 20 is electrically connected to the constant current source 18, the optical darkroom 9 is fixedly connected to the light source slide rail 26, and the optical detection module 21 is fixedly connected to the detection module mounting frame 19. On the mounting frame 19, the camera 24 is fixedly connected to one side surface of the camera fixing seat 25; the synchronous bracket 28 is also provided with a synchronous belt 30, a first pulley group, a synchronous motor 32, a synchronous slide rail 33 and a slide 34, the bottom end of the synchronous motor 32 is fixedly connected to the upper right end of the synchronous bracket 28, the first pulley group includes a first active pulley 31 and a first driven pulley 60, the first active pulley 31 is fixedly connected to the output end of the synchronous motor 32, and the first driven pulley 60 is installed on the left top end of the synchronous bracket 28, the inner side of the synchronous belt 30 is matched with the first pulley group, the synchronous slide rail 33 is arranged on the rear side of the synchronous belt 30, the slide 34 is slidably connected to the synchronous slide rail 33, and one side of the slide 34 is fixedly connected to the synchronous belt 30;The sample loading arm slide rail module includes a sample loading arm slide rail 11 and three electronic pipette guns 12 arranged side by side in front and back. The front end bottom of the sample loading arm slide rail 11 is slidably connected to the sliding bracket 27, and the rear end bottom surface thereof is fixedly connected to the top surface of the slide table 34. The upper inner side of the sample loading arm slide rail 11 is also provided with three sets of transmission mechanisms arranged side by side in the upper and lower parts. Taking one set as an example, the transmission mechanism includes a second pulley group, a slide plate 48, a transmission motor 49 and a transmission belt 50. The second pulley group includes a second active pulley 61 and a second driven pulley 4 7. The second driven pulley 47 is installed at the inner front end of the sample loading arm slide rail 11. One side of the transmission motor 49 is fixedly connected to the inner rear end of the sample loading arm slide rail 11. The output end thereof is fixedly connected to the second active pulley 61. The inner side of the transmission belt 50 is matched and connected with the second pulley group. One side of the slide plate 48 is slidably connected to the sample loading arm slide rail 11. It passes through the transmission belt 50 and is fixedly connected to one side of the transmission belt 50. The other side of the slide plate 48 is fixedly connected to the electronic liquid transfer gun 12 for filling samples and reagents. ;

[0039] Among them, a fully automatic platelet antibody detection analyzer and a method of using the same include the following steps:

[0040] (1) Before testing, the operator places the pipette tip 38 into the pipette tip box 37 (the system automatically records the remaining amount of the pipette tip 38 in the last test), places the platelet suspension, diluent and fluorescent reagent into the three reagent positions 6 of the reagent storage module from left to right, places the sample tube 39 into the sample rack 2, and places the corresponding number of gel cards 23 (each gel card 23 is for six people) in the gel card storage area according to the number of sample tubes 2 to be tested;

[0041] (2) Use the control system to set parameters such as the number of test samples, incubation temperature, incubation time, centrifugation speed, and centrifugation time (parameters can be named and saved);

[0042] (3) The control system performs the following operation instructions according to the number of samples:

[0043] ①Transmit the gel card 23 to the gel card storage puncture module to puncture the sealing film;

[0044] ② Use the sample loading arm slide module to sequentially add platelet suspension, diluent, fluorescent reagent and patient plasma sample into the gel card 23;

[0045] ③ Use the card clamping manipulator module to grab the gel card 23 from the gel card storage area and put it into the centrifugal incubation module; repeat the clamping action to put all the gel cards 23 with added samples into the centrifugal incubation module;

[0046] ④ After constant temperature incubation and centrifugation, the gel card 23 is placed in the detection module using a card clamping robot module;

[0047] ⑤ After the gel card 23 enters the detection module, it is first photographed and interpreted, and then transferred to the optical darkroom 9 through the light source slide rail 26 for fluorescence intensity detection, and the qualitative and quantitative detection results are given through the image processing algorithm and A / D amplification algorithm;

[0048] ⑥ After a gel card test 23 is completed, the gel card 23 is grabbed by the card clamping robot module and transferred to the test waste discarding module 4 for discarding. Then, the card clamping robot module is used to grab the gel card 23 from the centrifugal incubation module and put it into the detection module for detection. This process is repeated until all tests are completed.

[0049] Example

[0050] like Figures 1 to 7 As shown, before the test, the operator puts the pipette tip 38 into the pipette tip box 37 (the system automatically records the remaining amount of the pipette tip 38 in the last test), puts the platelet suspension, diluent and fluorescent reagent into the three reagent positions 6 of the reagent storage module from left to right, puts the sample tube 39 into the sample rack 2, and puts a corresponding number of gel cards 23 (six gel cards 23 per gel card 23) into the gel card storage area according to the number of sample tubes 2 to be tested; uses the control system to set parameters such as the number of test samples, incubation temperature, incubation time, centrifugation speed and centrifugation time (the parameters can be named and saved); the control system performs the following operating instructions according to the number of samples: transfers the gel card 23 to the gel card storage puncture module to puncture the sealing film; uses the sample loading arm slide rail module to add the platelet suspension, diluent, fluorescent reagent and patient plasma sample into the gel card 23 in turn; uses Use the card clamping robot module to grab the gel card 23 from the gel card storage area and put it into the centrifugal incubation module; repeat the clamping action to put all the gel cards 23 with added samples into the centrifugal incubation module; after the gel card 23 is incubated at a constant temperature and centrifuged, use the card clamping robot module to put it into the detection module; after the gel card 23 enters the detection module, it is first photographed and interpreted, and then transmitted to the optical darkroom 9 through the light source slide rail 26 for fluorescence intensity detection, and qualitative and quantitative detection results are given through the image processing algorithm and A / D amplification algorithm; after a gel card detection 23 is completed, the gel card 23 is grabbed by the card clamping robot module, and then rotated to the test waste discarding module 4 for discarding, and then the gel card 23 is grabbed from the centrifugal incubation module by the card clamping robot module and put into the detection module for detection, and this is repeated until all the detections are completed.

Claims

1. A method for using a fully automatic platelet antibody detection analyzer, characterized in that: The analyzer includes a control system, a circuit board, a base, a pipette tip storage module, a sample storage module, a gel card storage puncture module, a test waste discard module, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module and a sample loading arm slide rail module. The control system controls the operation of the circuit board, and the circuit board controls the actions of each module. The pipette tip storage module, the sample storage module, the gel card storage puncture module, the test waste discard module, the centrifugal incubation module, the reagent storage module, the card clamping manipulator module, the detection module and the beam module are all fixedly connected to the base, and are arranged from left to right along the length direction of the base. A pipette tip storage module, a sample storage module, a gel card storage puncture module, a test waste discarding module and a centrifugal incubation module are sequentially provided, the reagent storage module is located at the rear side of the pipette tip storage module, the circuit board is located at the rear side of the reagent storage module, the card clamping manipulator module is located at the rear side of the test waste discarding module, the detection module is located at the rear side of the centrifugal incubation module, the crossbeam module includes a sliding bracket and a synchronous bracket, the length direction of the sliding bracket and the synchronous bracket is parallel to the length direction of the base, and they are symmetrically arranged at the front and rear ends of the base respectively, the front end of the sample loading arm slide rail module is slidably connected to the sliding bracket, and the rear end thereof is installed on the synchronous bracket; The tip storage module is a drawer-type push-pull structure, and two tip boxes are arranged side by side in front and behind, and a certain number of tips are arranged in the tip boxes; The sample storage module is provided with six rows of sample racks arranged side by side on the left and right, each row of sample racks can accommodate twelve sample tubes, and each row of sample racks is provided with an independent slideway; The gel card storage puncture module includes a gel card holder, a track, a screw rod, a positioning motor, a slider, and a puncture mechanism. A certain number of gel card positions are arranged side by side on the top of the gel card holder, and a slider is fixedly connected to the bottom. The bottom surface of the slider abuts against the top surface of the track and can slide relatively. The positioning motor is fixedly connected to one end of the track, and its output end is fixedly connected to one end of the screw rod. The screw rod passes through the slider and is threadedly connected to it, and the other end of the screw rod is rotatably connected to the other end of the track. The puncture mechanism is located above the middle of the track and includes a puncture bracket, a puncture motor, an eccentric wheel, A puncturing rail, a puncturing slider, a tension spring and a knife seat, wherein the bottom end of the puncturing bracket is fixedly connected to the base, the puncturing motor is fixedly connected to the upper part of the puncturing bracket, and an eccentric wheel is fixedly connected to the output end thereof, there are two puncturing rails, which are symmetrically fixedly connected to the middle part of the puncturing bracket, and each of the puncturing rails is slidably connected to a puncturing slider, the knife seat is fixedly connected to the two puncturing sliders, and a certain number of puncturing knives are arranged on the knife seat, there are two tension springs, which are respectively located in front of the two puncturing rails, one end of the tension spring is fixedly connected to the puncturing bracket, and the other end of the tension spring is fixedly connected to the knife seat; The test waste discarding module is a drawer-type push-pull structure, and three sets of light pipes are arranged on both sides to facilitate monitoring whether the test waste discarding module is filled; The centrifugal incubation module includes a centrifuge and a temperature control module, the temperature control module includes a heating belt and a temperature sensor, the heating belt is arranged on the bottom surface of the centrifuge, and the temperature sensor is arranged on the top of the side of the centrifuge; The reagent storage module is provided with three reagent positions side by side on the left and right; The clamping robot module is provided with a clamping module mounting seat, a robot telescopic component, a robot mounting seat, a finger component, a robot rotating component and a rubber sleeve in sequence from bottom to top. The robot telescopic component includes a telescopic belt, a telescopic motor, a fourth pulley group and a lifting block. One side of the telescopic motor is horizontally fixedly connected to the bottom of the outer side of the clamping module mounting seat, and its output end passes through the clamping module mounting seat. The fourth pulley group includes a fourth active pulley and a fourth driven pulley. The fourth active pulley is fixedly connected to the output end of the telescopic motor, and the fourth driven pulley is installed on the top of the inner side of the clamping module mounting seat. The inner side of the telescopic belt is cooperatively connected with the fourth pulley group, and the lifting block is connected to the telescopic belt. One side of the belt is fixedly connected, the manipulator rotating component includes a rotating rod, a rotating belt, a rotating motor and a third pulley group, one side of the rotating motor is vertically fixedly connected to the upper part of the clamping module mounting seat, and its output end passes through the clamping module mounting seat, the two ends of the rotating rod are respectively rotatably connected to the clamping module mounting seat, the third pulley group includes a third active pulley and a third driven pulley, the third active pulley is fixedly connected to the output end of the rotating motor, the third driven pulley is fixedly connected to the top of the rotating rod, one end of the manipulator mounting seat is fixedly sleeved with a rubber sleeve, and the other end top surface is fixedly connected with a finger component, the rotating rod passes through the rubber sleeve and is slidably and frictionally connected thereto; The detection module includes a fixed plate, a constant current source, a detection module mounting frame, a light source, an optical detection module, an optical darkroom, a camera, a camera fixing seat, a light source slide rail and a backlight source. The constant current source, the detection module mounting frame, the camera fixing seat, the light source slide rail and the backlight source are all fixedly connected to the fixed plate, the light source is electrically connected to the constant current source, the optical darkroom is fixedly connected to the light source slide rail, the optical detection module is fixedly connected to the detection module mounting frame, and the camera is fixedly connected to a side surface of the camera fixing seat; The synchronous bracket is also provided with a synchronous belt, a first pulley group, a synchronous motor, a synchronous slide rail and a slide table. The bottom end of the synchronous motor is fixedly connected to the upper right end of the synchronous bracket. The first pulley group includes a first active pulley and a first driven pulley. The first active pulley is fixedly connected to the output end of the synchronous motor. The first driven pulley is installed on the left top end of the synchronous bracket. The inner side of the synchronous belt is cooperatively connected with the first pulley group. The synchronous slide rail is arranged at the rear side of the synchronous belt. The slide table is slidably connected with the synchronous slide rail, and one side of the slide table is fixedly connected with the synchronous belt. The loading arm slide module comprises a loading arm slide and three electronic pipetting guns arranged side by side in front and back, the front end bottom of the loading arm slide is slidably connected to the sliding bracket, and the rear end bottom surface is fixedly connected to the top surface of the slide, and the upper inner side of the loading arm slide is also provided with three groups of transmission mechanisms arranged side by side up and down, taking one group as an example, the transmission mechanism comprises a second pulley group, a slide plate, a transmission motor and a transmission belt, the second pulley group comprises a second active pulley and a second driven pulley, the second driven pulley is installed on the inner front end of the loading arm slide, one side of the transmission motor is fixedly connected to the inner rear end of the loading arm slide, and the output end thereof is fixedly connected with the second active pulley, the inner side of the transmission belt is cooperatively connected with the second pulley group, one side of the slide plate is slidably connected to the loading arm slide, it passes through the transmission belt and is fixedly connected to one side of the transmission belt, and the other side of the slide plate is fixedly connected to the electronic pipetting gun; The method of use comprises the following steps: (1) Before testing, the operator places the pipette tip into the pipette tip box, and the system automatically records the remaining amount of the pipette tip from the last test. The platelet suspension, diluent, and fluorescent reagent are placed in the three reagent positions of the reagent storage module from left to right, and the sample tube is placed in the sample rack. According to the number of sample tubes to be tested, the corresponding number of gel cards are placed in the gel card storage area, and each gel card is for six people; (2) Use the control system to set parameters such as the number of test samples, incubation temperature, incubation time, centrifugation speed and centrifugation time. The parameters can be named and saved; (3) The control system performs the following operation instructions according to the number of samples: ①Transfer the gel card to the gel card storage puncture module to puncture the sealing film; ② Use the sample loading arm slide module to add platelet suspension, diluent, fluorescent reagent and patient plasma sample to the gel card in sequence; ③ Use the card clamping robot module to grab the gel card from the gel card storage area and put it into the centrifugal incubation module; repeat the clamping action to put all the gel cards with added samples into the centrifugal incubation module; ④After constant temperature incubation and centrifugation, the gel card is placed into the detection module using the card clamping robot module; ⑤ After the gel card enters the detection module, it is first photographed and interpreted, and then transferred to the optical darkroom through the light source slide rail for fluorescence intensity detection, and the qualitative and quantitative detection results are given through the image processing algorithm and A / D amplification algorithm; ⑥ After a gel card test is completed, use the card clamping robot module to grab the gel card, turn it to the test waste disposal module for disposal, and then use the card clamping robot module to grab the gel card from the centrifugal incubation module and put it into the detection module for detection, and repeat this process until all tests are completed.

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