A method for preparing a mixing-promoting microfluidic channel based on an air-mode method

Abstract

The invention discloses a method for preparing a mixing-promoting microfluidic channel based on an air mold method, which relates to the field of microfluidic devices. A plurality of microporous structures are provided on a protruding structure; a liquid polymer film that can be heat-cured or light-cured is coated on an auxiliary plate and a gap block is placed on the auxiliary plate; a side of the template with a protruding structure contacts the liquid polymer on the auxiliary plate, and the template is supported by the gap block; the template and the auxiliary plate are kept in the matching state and sent into a vacuum drying oven; the vacuum drying oven pressure is adjusted and the liquid polymer is heated and cured, and the cured polymer is a spherical crown-shaped mixing-promoting microfluidic body; the spherical crown-shaped mixing-promoting microfluidic body is bonded to a glass slide or a polymer surface, thereby obtaining a spherical crown-shaped mixing-promoting microfluidic channel. The use of an inverted form of a forming polymer and a template can effectively reduce the number of micropores filled by the forming polymer, and the microfluidic channel and the mixing-promoting spherical crown-shaped cavity are formed in one forming process by using a replica molding method and an air mold method.

Description

Technical Field

[0001] The invention relates to the field of microfluidic devices, and in particular to a method for preparing a mixing-promoting microfluidic channel based on an air modeling method. Background Art

[0002] Microfluidic chips are microchannels formed by etching or molding on different materials such as glass, silicon or polydimethylsiloxane. In the past decade, microfluidic chips have received a lot of attention, especially in the field of biotechnology. Using microfluidic chips to sort biological particles such as cells and microorganisms has become one of the most basic research topics in biotechnology microfluidics.

[0003] In existing microfluidic chips, microcavity structures are mainly realized using traditional photolithography technology, and the patterns formed are mainly rectangular or circular cavities. Ngan Nguyen et al. (Adv. Funct. Mater. 2019, 29, 1901998) proposed a method of adding gallium-based alloy droplets as a template at an ideal position in the channel to form a spherical crown structure with a diameter of several hundred microns, and found that this structure has the function of promoting microfluid mixing. However, this method has certain limitations. When adding droplets, it is necessary to add drop by drop, which is inefficient, and the amount and position accuracy of the droplets added are required to be high; the use of gallium-based alloy droplets is easy to oxidize; the opening of the spherical crown structure formed is not easy to control, and can only be circular in shape and the opening diameter is related to the spherical crown structure. The gas mold forming method disclosed in Chinese Patent 200910024713.0 is a method of realizing polymer microstructure by adjusting the expansion of a closed gas. The gas mold method has the advantages of high efficiency, batch production, and controllability. The template of the gas mold forming method needs to be prepared by reactive ion etching process and the adjustable range of the template microstructure shape is narrow (Biomed Microdevices 2014, 16: 55–67).

[0004] In summary, the existing gallium-based alloy droplet forming method for preparing mixing-promoting spherical crown cavities on microfluidic channels is difficult to adapt to the needs of mass production, and the process is complex and has poor controllability. Summary of the invention

[0005] In view of the shortcomings in the prior art, the present invention provides a method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method, which can achieve efficient, low-cost and controllable preparation of a spherical crown-shaped mixing-promoting microfluidic channel of polymer materials under simple conditions.

[0006] The present invention achieves the above technical objectives through the following technical means.

[0007] A method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method comprises the following steps:

[0008] The template has a protruding structure, and a plurality of microporous structures are opened on the protruding structure;

[0009] coating a heat-curable or light-curable liquid polymer film on the auxiliary flat plate and placing a gap block on the auxiliary flat plate;

[0010] The side of the template with the raised structure is in contact with the liquid polymer on the auxiliary plate, and the template is supported by the gap block;

[0011] The template and the auxiliary plate are sent into a vacuum drying oven while maintaining the matching state;

[0012] The pressure of the vacuum drying oven is adjusted and the liquid polymer is cured by heating or light exposure, and the cured polymer is a spherical crown-shaped mixing-promoting microfluid body;

[0013] The spherical crown-shaped mixing-promoting microfluidic body is bonded to a glass slide or a polymer surface to obtain a spherical crown-shaped mixing-promoting microfluidic channel.

[0014] In the above scheme, the height of the protruding structure is 5 μm to 200 μm, the micropore depth in the micropore structure is greater than the micropore diameter, and the micropore diameter is smaller than the width of the protruding structure, wherein the micropore structure is a blind hole.

[0015] In the above solution, the thickness of the liquid polymer film is 50 μm to 1500 μm.

[0016] In the above solution, the gap blocks are arranged at the four corners of the auxiliary plate, and the positions where the gap blocks are placed are not coated with the liquid polymer film; the height of the gap blocks is less than the thickness of the liquid polymer film.

[0017] In the above scheme, the vacuum drying oven pressure P is:

[0018]

[0019] Wherein, h is the micropore depth; r is the micropore opening radius; R is the curvature radius of the spherical crown-shaped mixing chamber; P0 is the atmospheric pressure; when the micropore opening is a special-shaped structure, r is the micropore equivalent opening radius, S-micropore opening area.

[0020] In the above scheme, the spherical crown-shaped mixing-promoting microfluidic channel body and the glass slide or polymer surface to be bonded are first treated by plasma, and then the treated spherical crown-shaped mixing-promoting microfluidic channel body surface and the glass slide or polymer surface are directly contacted and heat-baked at 80°C for 15 to 30 minutes.

[0021] In the above scheme, all the outwardly opening areas of the spherical crown-shaped mixing-promoting microfluidic channel body except the two longitudinal ends of the microfluidic channel are sealed with a glass slide or a polymer surface.

[0022] In the above scheme, the crown-shaped mixing-promoting microfluid body includes a spherical crown-shaped mixing-promoting cavity and a microfluidic channel; the intersection line of the crown-shaped mixing-promoting cavity and the microfluidic channel can be in the shape of a triangle, a rectangle, an ellipse or a cross.

[0023] In the above scheme, the microporous structure is oval, circular, square, cross-shaped or triangular.

[0024] In the above solution, the liquid polymer material is a heat-curable or light-curable polymer material, preferably polydimethylpolysiloxane.

[0025] The present invention has the following technical advantages:

[0026] 1. The forming method of the present invention simultaneously processes the template protrusion structure for forming the microfluidic channel and the micropores for forming the mixing-promoting spherical crown cavity on the same template. On this basis, the forming polymer and the template are inverted to effectively reduce the filling of the micropores by the forming polymer, so that the microfluidic channel and the mixing-promoting spherical crown cavity can be formed respectively by using the replica molding method and the gas molding method in one molding process.

[0027] 2. The present invention realizes the preparation of a spherical crown-shaped mixing-promoting microfluidic channel through an air modeling method. The prepared spherical crown-shaped structure is controlled by vacuum pressure, thereby realizing the efficient, low-cost and controllable preparation of a spherical crown-shaped mixing-promoting microfluidic channel.

[0028] 3. The preparation of the spherical crown-shaped mixing-promoting microfluidic channel is achieved by the air molding method. The opening shape characteristics of the spherical crown-shaped mixing-promoting cavity can be controlled by adjusting the shape of the micropore opening on the protruding structure on the template.

[0029] 4. The preparation of the spherical crown-shaped mixing-promoting microfluidic channel is achieved by the air mold method, and the orientation relationship between the spherical crown-shaped mixing-promoting cavity and the microfluidic channel can be adjusted by adjusting the orientation of the template. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a schematic diagram of the process of implementing the method for preparing a spherical crown-shaped mixing-promoting microfluidic channel based on an air model method according to an embodiment of the present invention;

[0031] Figure 2 It is a schematic diagram of the template structure;

[0032] Figure 3 This is a schematic diagram of the product structure;

[0033] Figure 4 It is a schematic diagram of the structure of a prismatic opening template;

[0034] Figure 5 It is a schematic diagram of the structure of a prismatic opening product;

[0035] Figure 6 A schematic diagram of placement of the auxiliary plate-forming polymer-gap block-template system when the template surface is not parallel to the horizontal plane;

[0036] Figure 7 The spherical crown-shaped mixing microfluidic body is attached Figure 3 A schematic diagram of a cross-sectional structure perpendicular to the groove;

[0037] Figure 8 The spherical crown-shaped mixing microfluidic body is attached Figure 3 A schematic diagram of the cross-sectional structure along the center line of the groove;

[0038] Fig. 9 It is a schematic diagram of the morphology and structure of the square-opened spherical crown-shaped mixing chamber;

[0039] Fig.10 This is a schematic diagram of the morphology and structure of the elliptical open spherical crown-shaped mixing chamber.

[0040] Reference numerals:

[0041] 1-auxiliary plate, 2-liquid polymer film, 3-gap block, 4-template plate, 5-template, 6-protrusion structure, 7-micropore, 8-microbubble, 9-spherical crown-shaped mixing-promoting microfluid body, 10-microfluid channel, 11-spherical crown-shaped mixing-promoting cavity, 12-bonding base plate, 13-circular hole protrusion structure template, 14-first long strip protrusion structure, 15-circular micropore, 16-circular hole protrusion structure template corresponding to the product, 17-circular hole protrusion structure template corresponding to the product groove, 18-spherical crown-shaped mixing-promoting cavity with circular opening, 19-prismatic opening template, 20-second long strip protrusion structure, 21-prismatic opening hole, 22-prismatic opening template corresponding to the product, 23-prismatic opening template corresponding to the product groove, 24-spherical crown-shaped mixing-promoting cavity with prismatic opening, 25-shaped polymer container, 26-spherical crown-shaped mixing-promoting microfluid body wall. DETAILED DESCRIPTION

[0042] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0043] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "axial", "radial", "vertical", "horizontal", "inner", "outer" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0044] The purpose of the present invention is to provide a method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method, so as to achieve efficient, low-cost and controllable preparation of a spherical crown-shaped mixing-promoting microfluidic channel of a polymer material under simple conditions.

[0045] A method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method is carried out according to the following steps:

[0046] (1) preparing a template having a long strip-shaped protrusion structure with a microporous structure on the surface;

[0047] (2) evenly spreading a layer of a heat-curable or light-curable liquid polymer film to be formed on an auxiliary flat plate;

[0048] (3) Place gap blocks on the auxiliary plate;

[0049] (4) placing the template with a long strip protrusion structure having a microporous structure on an auxiliary plate spread with a liquid polymer film, so that the blank portion of the template surface contacts the gap block on the auxiliary plate, and keeping this state, sending it into a vacuum drying oven;

[0050] (5) setting the pressure of the vacuum drying oven according to the designed pressure value, thereby heating and curing the polymer material;

[0051] (6) separating the template and the solidified polymer to achieve the preparation of a spherical crown-shaped mixing-promoting microfluidic body;

[0052] (7) Bonding the spherical crown-shaped mixing-promoting microfluidic channel body to a glass slide or a polymer surface to obtain a spherical crown-shaped mixing-promoting microfluidic channel.

[0053] In step (1) of the above method, it is necessary to first prepare a template of a long strip-shaped protrusion structure with a microporous structure. It can be prepared by traditional micromachining methods such as laser direct writing processing methods and mechanical processing methods. The prepared protrusion height is between 5μm and 200μm, and the depth of the micropores prepared on the protrusion structure is greater than the diameter of the micropores, and the diameter of the micropores is smaller than the width of the protrusion structure.

[0054] In step (3) of the above method, gap blocks 3 are placed on the auxiliary plate 1, and four gap blocks 3 with a thickness of 300μm to 1500μm less than the thickness of the liquid polymer film are prepared. The length and width of the gap blocks 3 range from 1×1mm to 5×5mm. Four gap blocks 3 are placed on the auxiliary plate 1 and the four gap blocks 3 are dispersed to form a rectangle between the gap blocks 3, ensuring that the distribution range of the gap blocks 3 is smaller than the template range but larger than the distribution range of the protruding structure 6 on the template 5.

[0055] In step (4) of the above method, the template with the long strip protrusion structure having a microporous structure is placed on an auxiliary plate 1 spread with a liquid polymer film, so that the blank area on the template surface contacts the gap block 3 on the auxiliary plate, and the liquid polymer 2 contacts the surface of the long strip protrusion structure having a microporous structure, thereby achieving liquid sealing of the gas in the microporous structure on the surface of the long strip protrusion structure, and the state is maintained and sent into a vacuum drying oven.

[0056] Combined with Figure 1 As shown, the method for preparing a spherical crown-shaped mixing-promoting microfluidic channel based on an air model method comprises the following steps:

[0057] First, the template plate 4 is processed into a template 5 by a conventional micro-machining method such as a laser direct writing method and a mechanical processing method. The template 5 is provided with a long strip-shaped protruding structure 6, and a plurality of micro-holes 7 are provided on the protruding structure 6.

[0058] Prepare an auxiliary plate 1 and evenly spread a layer of liquid polymer 2 on the auxiliary plate 1, and spread the liquid polymer film to the required thickness, such as 50 to 1500 μm, by a glue spreader or by gravity; the spreading area of ​​the liquid polymer film can completely cover the surface of the long strip convex structure with a microporous structure.

[0059] Then, gap blocks 3 are placed on the auxiliary plate 1 to control the thickness of the final formed product; when placing the gap blocks 3, the spacing between the multiple gap blocks 3 needs to be controlled to ensure that the processed template 5 can be stably placed on the gap blocks 3 and that the gap blocks 3 do not cause spatial interference with the protruding structures 6 on the template 5;

[0060] The template 5 is mounted on the gap block 3 so that the protruding structure 6 on the template 5 is in close contact with the liquid polymer 2. At this time, the residual gas inside the micropores 7 is sealed by the liquid polymer 2. The matched template 5 and the auxiliary plate 1 are sent into the vacuum drying oven. According to the micropore 7 hole depth h, the micropore opening radius r and the curvature radius R of the designed spherical crown-shaped mixing chamber 11, the pressure of the vacuum drying oven is calculated:

[0061]

[0062] Wherein, P0-atmospheric pressure, the vacuum drying oven is evacuated to the calculated pressure value P, and after evacuation, microbubbles 8 will be generated at the positions corresponding to the micropores in the formed polymer;

[0063] The liquid polymer is solidified by using a corresponding process according to the instructions for use of the formed polymer. When polydimethylpolysiloxane (PDMS) of the preferred embodiment is used, the vacuum drying oven is heated to 60° C. and kept warm for 2 hours to solidify the formed polymer film, and then cooled naturally after the solidification process is completed;

[0064] The solidified polymer is separated from the template 5 and the auxiliary plate 1, and a spherical crown-shaped mixing-promoting microfluid body 9 is obtained. The protruding structure 6 will replicate a microfluidic channel 10, and the microbubbles 8 are solidified to become a spherical crown-shaped mixing-promoting cavity 11;

[0065] The spherical crown-shaped mixing-promoting microfluidic body 9 and the bonding base plate 12 are treated by plasma, wherein the bonding base plate 12 is a glass slide or a polymer surface, and then the treated spherical crown-shaped mixing-promoting microfluidic body 9 and the bonding base plate 12 are directly contacted with each other, and heat baking at 80°C for 15 to 30 minutes is sufficient to obtain a strong bonding strength, so that the bonding base plate 12 seals all areas of the spherical crown-shaped mixing-promoting microfluidic channel body 9 that are open to the outside except for the longitudinal ends of the microfluidic channel, thereby obtaining a spherical crown-shaped mixing-promoting microfluidic channel.

[0066] The shape of the intersection line between the spherical crown-shaped mixing chamber 11 and the microfluidic channel 10 can be adjusted to a triangular, rectangular, elliptical, cross-shaped or other special shapes, which can be achieved by adjusting the opening shape of the micropores 7 on the protruding structure 6, see the attached Figures 2 to 5 .

[0067] Specifically, when the template is a round hole protrusion structure template 13, and the circular micropores 15 on the first long strip protrusion structure 14 on the round hole protrusion structure template 13 are round holes, the obtained round hole protrusion structure template corresponding product 16 has a round hole protrusion structure template corresponding product groove 17 and a round opening spherical crown-shaped mixing chamber 18;

[0068] When the template is a prismatic opening template 19, that is, the prismatic opening template 18 has a second long strip protrusion structure 20 and a prismatic opening hole 21, the formed product is a prismatic opening template corresponding product 22, which has a prismatic opening template corresponding product groove 23 and a prismatic opening spherical crown-shaped mixing chamber 24. The adjustment method of other special-shaped connecting holes is similar to the above description.

[0069] When the opening shape of the micropores 7 on the protruding structure 6 on the template 5 is a special shape, the calculation method of the vacuum drying box pressure is as follows: first, the micropore equivalent opening radius r is taken as Where S is the opening area; then according to the formula

[0070]

[0071] The pressure of the vacuum drying oven is calculated, where P0 is atmospheric pressure, h is micropore depth, and R is the radius of curvature of the designed spherical crown-shaped mixing chamber.

[0072] The directional relationship between the spherical crown-shaped mixing chamber 11 and the microfluidic channel 10 can be adjusted by adjusting the angle between the template plate 4 and the horizontal plane before the polymer is solidified. Figure 6 In order to prevent the flow of the forming polymer from affecting the forming process, the auxiliary plate 1-liquid polymer 2-gap block 3-template 5 system is placed in a container 25 containing the forming polymer. The inclined position relationship between the spherical crown-shaped mixing chamber 11 and the microfluidic channel 10 can be achieved by adjusting the angle between the template surface and the horizontal plane before the polymer is solidified. When the angle is 0° (the template surface is the horizontal plane), the spherical crown-shaped mixing chamber and the microfluidic channel are in a vertical position, and when the angle deviates from 0°, it is in an inclined position.

[0073] Example

[0074] The liquid polymer 4 is made of polydimethylpolysiloxane (PDMS), the auxiliary plate 1 is made of a smooth Si surface, and the gap block 3 is made of silicon wafer fragments.

[0075] First, the surface of the 1060 aluminum plate substrate is polished and used as a template plate 4. Then, a template 5 with a long strip convex structure is prepared by laser direct writing. The diameter of the laser spot used is 20 μm. The overlap rate of the laser spot during scanning is 50%, that is, the distance between two consecutive spots is 10 μm. The two sides of the long strip convex structure are scanned to remove the material around the convex structure. The number of scans is 2 times, leaving the middle convex structure. After processing, the middle convex structure has a width of 1 mm and a length of 10 mm. The width of the two sides is 8 mm and the depth is 100 μm. Laser polishing is then performed. Then, a microhole array with a diameter of 100 μm, a depth of 120 μm, and a spacing of 1 mm is processed on the long strip convex structure. 300 μL of forming polymer polydimethylpolysiloxane (PDMS), purchased from Dow Corning, USA, trade name Sylgard184A, was dropped onto the auxiliary smooth Si surface, and the PDMS was allowed to spread freely for 3 minutes, at which time a smooth and straight silicon wafer coated with a liquid PDMS film was obtained. Four pieces of silicon wafer fragments with a thickness of 300 μm were placed on the edge of the smooth and straight silicon wafer as gap blocks, and the prepared 1060 aluminum plate with a long strip convex structure of micropores was placed horizontally and inverted on the gap blocks, so that the PDMS film sealed the residual gas inside the micropores on the long strip convex structure. Keep the smooth and straight silicon wafer horizontal, together with the liquid PDMS and 1060 aluminum plate, and put it into a vacuum drying oven. Set the pressure in the vacuum drying oven to 0.08MPa, heat it to 60℃ and keep it warm for 2 hours. After the end, cool it naturally, and separate the polymer film from the auxiliary plate 1 and the convex structure surface with the micropore array. Bond the PDMS polymer film to the glass plasma. During the plasma treatment, heat it at 80℃ for 15 to 30 minutes to obtain a strong bonding strength. Finally, a spherical crown-shaped mixing-promoting microfluidic channel with a channel size of 1mm in width and 100μm in height is obtained. The cross-sectional shape and side shape of the spherical crown-shaped mixing-promoting microfluidic body 9 are as shown in the attached figure. Figure 7 As shown in FIG. 1 , the microfluidic channel 10 and the spherical cap-shaped mixing chamber 11 are included. The microfluidic channel 10 has a relatively uniform spherical cap-shaped mixing microfluid main body wall 26. Similarly, the spherical cap-shaped mixing chamber 11 obtained by opening square micropores and elliptical micropores on the convex structure is shown in FIG. Fig. 9 and attached Fig.10 shown.

[0076] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0077] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and intent of the present invention.

Claims

1. A method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air model method, characterized in that: The steps include: The template has a protruding structure, and a plurality of microporous structures are opened on the protruding structure; coating a heat-curable or light-curable liquid polymer film on the auxiliary flat plate and placing a gap block on the auxiliary flat plate; The side of the template with the protruding structure contacts the liquid polymer on the auxiliary plate, and the template is supported by the gap block to form a mating state; The template and the auxiliary plate are sent into a vacuum drying oven while maintaining the matching state; The vacuum drying oven pressure is adjusted and the liquid polymer is cured by heating or light, and the cured polymer is a spherical crown-shaped mixing-promoting microfluid body; wherein the spherical crown-shaped mixing-promoting microfluid body comprises a spherical crown-shaped mixing-promoting cavity and a microfluid channel; the intersection line of the spherical crown-shaped mixing-promoting cavity and the microfluid channel is in the shape of a triangle, a rectangle, a circle, an ellipse or a cross; The spherical crown-shaped mixing-promoting microfluidic body is bonded to a glass slide or a polymer surface to obtain a spherical crown-shaped mixing-promoting microfluidic channel.

2. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: The height of the protruding structure is 5 μm to 200 μm, the micropore depth in the micropore structure is greater than the micropore diameter, and the micropore diameter is smaller than the width of the protruding structure, wherein the micropore structure is a blind hole.

3. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: The thickness of the liquid polymer film is 50 μm to 1500 μm.

4. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: The gap blocks are arranged at the four corners of the auxiliary plate, and the positions where the gap blocks are placed are not coated with the liquid polymer film; the height of the gap blocks is less than the thickness of the liquid polymer film.

5. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: Vacuum drying oven pressure P: Wherein, h is the micropore depth; r is the micropore opening radius; R is the curvature radius of the spherical crown-shaped mixing chamber; P0 is the atmospheric pressure; when the micropore opening is a special-shaped structure, r is the micropore equivalent opening radius, S-micropore opening area.

6. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: First, the spherical crown-shaped mixing microfluidic channel body and the glass slide or polymer surface to be bonded are treated by plasma, and then the treated spherical crown-shaped mixing microfluidic channel body surface and the glass slide or polymer surface are directly contacted and heat-baked at 80°C for 15 to 30 minutes.

7. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 6, characterized in that: Use a glass slide or a polymer surface to seal all the outwardly opened areas of the spherical crown-shaped mixing-promoting microfluidic channel body except for the two longitudinal ends of the microfluidic channel.

8. The method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to claim 1, characterized in that: The micropore structure is oval, circular, square, cross or triangular.

9. According to the method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method according to any one of claims 1 to 8, the liquid polymer material is a heat-curable or light-curable polymer material. 10 . According to the method for preparing a polymer spherical crown-shaped mixing-promoting microfluidic channel based on an air modeling method as claimed in claim 9 , the liquid polymer material is polydimethylpolysiloxane.

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