Plant expression vector and application thereof in degradation of rice lignin

A plant expression vector and vector technology, applied in the field of bioengineering, can solve the problems of long strain culture period, limited popularization and application, lignin loss, etc.

CN114854788APending Publication Date: 2022-08-05ZHENGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2022-08-05

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a plant expression vector and application thereof in degradation of rice lignin. According to the plant expression vector, a GRP gene, an SHP gene and a GLOX gene are connected to a pCAMBIA3301 vector, the sequence of the GRP gene is as shown in SEQ ID NO.4, the sequence of the SHP gene is as shown in SEQ ID NO.3, and the GLOX gene is as shown in SEQ ID NO.7. The invention further discloses a preparation method of the plant expression vector. When the plant expression vector is used for transforming rice, the lignin content of the obtained transgenic rice in the full ripening stage is reduced, the lignin content change in the flowering stage is not large, and the problems that lignin is lost in the crop growth process, and popularization and application of a biological treatment method in production are limited are solved.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a plant expression vector and its application in degrading rice lignin. Background technique

[0002] Lignocellulosic resources are abundant renewable energy materials in nature. As the main carrier of biomass energy, their research and application have been widely concerned at home and abroad. Lignin is one of the three main components of the plant cell wall. It is surrounded or bound in the cellulose framework, which can enhance the mechanical strength of the plant body, and at the same time is beneficial to the water transport of the transport tissue and resist the adverse invasion of the external environment. The complexity of its structure and its stable form in vivo make it difficult to be degraded, industrially separated or transformed, and it has become the primary barrier for the utilization of lignocellulose resources.

[0003] The key to improving t...

Examples

Embodiment Construction

[0044] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

[0045] The present invention utilizes genetic engineering technology, is based on common plant expression vectors, uses glycine-rich protein (GRP) in rice genome as signal peptide, and uses soybean-derived hull peroxidase (Soybean hullperoxidase, SHP) and ethyl acetate. Glyoxal oxidase (GLOX) was used as the target expression product, and a directional expression vector was constructed to promote soybean husk peroxidase to cross the cell membrane and target the cell wall, so that the endogenous SHP enzyme o...