A highly efficient and clean organic material decomposing bacterial agent and its preparation method and application

Abstract

The present invention relates to an efficient and clean organic material decomposing agent and a preparation method and application thereof, which is composed of jelly-like Bacillus powder and griseobacillus rhodopsin powder; the griseobacillus rhodopsin powder accounts for 40% to 60% of the total mass of the organic material decomposing agent; the fermented bacteria of jelly-like Bacillus and griseobacillus rhodopsin and their products are prepared by solid-state fermentation, dried and crushed to obtain powders, and mixed evenly to obtain an efficient and clean organic material decomposing agent, which is stored in a cool and dry place. The organic material decomposing agent is mixed with soil and then spread on the organic material, which is turned over and covered with soil, and watered. After the organic material is decomposed for at least one week, wheat seeds can be sown on the soil buried with the decomposed organic material for planting wheat. The decomposing agent of the present invention can efficiently and cleanly degrade organic materials, and the decomposed organic materials can be used as organic fertilizers in crop production to increase crop yields.

Description

Technical Field

[0001] The invention relates to the technical field of organic material processing and organic fertilizer, and in particular to a highly efficient and clean organic material decomposing bacterial agent and a preparation method and application thereof. Background Art

[0002] The world can provide 1.7 billion tons of various straws every year, of which less than 2% is used. In my country's agricultural production, the annual straw output is 570 million tons, accounting for 20%-30% of the world's total straw output, but less than 33.3% is used, of which only 2.6% is used with high added value. Most of them are simply processed as livestock feed, composted and returned to the field, and biogas fermentation, without giving full play to the value of straw. The inability to effectively utilize straw resources is not only a huge waste of resources, but also related to environmental protection issues. Most of the unused straw is burned, and straw stores a large amount of carbon, which will be converted into greenhouse gas carbon dioxide after burning, exacerbating the greenhouse effect. Reasonable use of straw resources can fix the carbon in them and greatly reduce the emission of carbon dioxide in the atmosphere.

[0003] Burning straw is generally done on-site, and the high temperature generated during the burning process has a great killing effect on the microorganisms in the soil. In addition, burning straw will cause soil moisture to evaporate, which is not conducive to the survival of soil microorganisms, and ultimately lead to soil structure damage, such as soil compaction, decreased fertility, deterioration of the soil ecosystem, and decreased crop yields. Therefore, the comprehensive utilization of straw has become an important solution to the problem of agricultural environment and resource utilization, and the rational treatment of straw is of great significance to agricultural development. Summary of the invention

[0004] In order to solve the technical problems of waste of resources and environmental pollution caused by improper treatment of organic materials, an efficient and clean organic material decomposing bacteria agent and its preparation method and application are provided. The organic material decomposing bacteria agent of the present invention can efficiently degrade a variety of organic materials, including rice straw, asparagus straw, corn straw and succulent straw with skin and leaves, etc. The preparation method is simple and efficient. The decomposed organic materials can be used as organic fertilizer in crop production, which can increase crop yield.

[0005] In order to achieve the above objectives, the present invention is implemented by the following technical solutions:

[0006] The invention discloses an efficient and clean organic material decomposing bacterial agent, which is composed of gelatinous Bacillus powder and griseorhizium powder and is used for decomposing organic materials.

[0007] Furthermore, the Streptomyces griseus powder accounts for 40% to 60% of the total mass of the organic material decomposing bacteria agent.

[0008] Furthermore, the Streptomyces griseus powder accounts for 60% of the total mass of the organic material decomposing bacteria agent.

[0009] On the other hand, the present invention provides a highly efficient and clean organic material decomposing bacteria agent and a preparation method thereof, comprising the following steps: preparing fermented bacteria bodies of Bacillus jelly and Streptomyces griseus and their products respectively by solid-state fermentation, obtaining Bacillus jelly powder and Streptomyces griseus powder after drying and crushing, mixing the two evenly to obtain the organic material decomposing bacteria agent, and storing the mixture in a cool and dry place.

[0010] Furthermore, the preparation process of the jelly-like bacillus powder is as follows: inoculating the jelly-like bacillus on a first activation culture medium, placing it in a constant temperature dark incubator at 28-32°C for 1-2 days for activation and obtaining a jelly-like bacillus bacterial liquid; then inoculating the bacterial liquid on a first liquid seed culture medium, and culturing it on a shaking table at 30°C and 200rpm for 2 days to obtain a jelly-like bacillus seed liquid; inoculating the seed liquid on a first solid culture medium for fermentation culture, and fermenting it at a constant temperature of 32°C for 7 days. At the end of the fermentation culture, the moisture content is 30% and the number of effective live bacteria is 300 million / g. The fermented bacterial bodies of the jelly-like bacillus and their products are collected, and crushed to obtain the jelly-like bacillus powder;

[0011] The preparation process of the Streptomyces griseus powder is as follows: inoculating the Streptomyces griseus on a second activation culture medium, placing the culture in a constant temperature dark incubator at 28-35°C for 3-5 days for activation and obtaining a Streptomyces griseus bacterial liquid; then inoculating the bacterial liquid on a second liquid seed culture medium, and performing shaking culture at 32°C and 200 rpm for 5 days to obtain a Streptomyces griseus seed liquid; inoculating the seed liquid on a second solid culture medium for fermentation culture, and fermenting at a constant temperature of 35°C for 5 days. At the end of the fermentation culture, the moisture content is 15% and the number of effective live bacteria is 500 million / g. The fermented cells of the Streptomyces griseus and their products are collected, and crushed to obtain the Streptomyces griseus powder.

[0012] Furthermore, the formula of the first activation medium is 20.0 g of glucose, 16.7 g of peptone, 5.3 g of NaCl, 5.3 g of beef extract, and 15 g of agar per liter of distilled water, pH = 7.0;

[0013] The formula of the first liquid seed culture medium is 20.0 g glucose, 16.7 g peptone, 5.3 g NaCl, and 5.3 g beef extract per liter of distilled water, pH = 7.0;

[0014] The first solid culture medium is composed of 90% bran, 9.8% rice bran, 0.05% MgSO4·7H2O, 0.05% NH4SO4, and 0.1% CaCO3 in mass percentage, with a pH of 7.0. Water is added in equal weight and mixed, and sterilized at 121° C. for 1 hour before use. The carrier matrix in the solid culture medium is improved, so that the number of effective live bacteria is significantly increased.

[0015] The inoculation amount of the jelly-like Bacillus on the first activation culture medium is one ring of jelly-like Bacillus inoculated in a 20mL culture dish; the inoculation amount of the jelly-like Bacillus liquid on the first liquid seed culture medium is 1mL / 200mL; and the inoculation amount of the jelly-like Bacillus seed liquid on the first solid culture medium is 150mL / kg.

[0016] Furthermore, the formula of the second activation medium is 20.0 g of soluble starch, 1.0 g of KNO3, 0.5 g of K2HPO4, 0.5 g of MgSO4·7H2O, 0.5 g of NaCl, and 15 g of agar per liter of distilled water, with a pH of 7.2;

[0017] The formula of the second liquid seed culture medium is 20.0 g soluble starch, 1.0 g KNO3, 0.5 g K2HPO4, 0.5 g MgSO4·7H2O, and 0.5 g NaCl per liter of distilled water, with a pH of 7.2;

[0018] The second solid culture medium is composed of rice bran 99%, KNO3 0.1%, K2HPO4 0.05%, MgSO4·7H2O 0.05%, NaCl 0.05%, CaCO3 0.75% by mass percentage, pH = 7.2, water is added in equal weight, mixed and sterilized at 121°C for 1 hour before use; the carrier matrix in the solid culture medium is improved, so that the number of effective live bacteria is significantly increased;

[0019] The inoculation amount of the Streptomyces griseus on the second activation culture medium is two loops in a 20 mL culture dish; the inoculation amount of the Streptomyces griseus bacterial solution on the second liquid seed culture medium is 5 mL / 200 mL; and the inoculation amount of the Streptomyces griseus seed solution on the second solid culture medium is 200 mL / kg.

[0020] The last aspect of the present invention provides the application of the above-mentioned efficient and clean organic material decomposing bacteria agent, which is mixed with soil and then spread on the organic material, which is then turned over and covered with soil and watered. After the organic material has been decomposed for at least one week, wheat seeds can be sown on the soil buried with decomposed straw for planting wheat; the mass ratio of the organic material to the organic material decomposing bacteria agent is 250:1.

[0021] Furthermore, urea is added to the organic material and the decomposing bacteria agent, and the mass ratio of the organic material, the decomposing bacteria agent and the urea is 250:1:2.5. Urea is added to balance the carbon-nitrogen ratio in the soil.

[0022] Beneficial technical effects:

[0023] The present invention adopts a compound of Bacillus gelatinus and Streptomyces griseus as an organic material decomposing agent, which has the function of synergistically and efficiently degrading organic materials, effectively improving the degradation efficiency of the whole process of organic material decomposition, and using the optimized combination of the two strains of bacteria can greatly shorten the decomposition time of organic materials. After the organic materials to which the decomposing agent is added are decomposed in situ, the soil structure and fertility can be improved, soil-borne diseases can be inhibited, and the yield and quality of crops can be increased, which has important economic value and far-reaching ecological significance. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Unless otherwise specifically stated, the numerical value set forth in these embodiments does not limit the scope of the present invention. The technology and method known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology and method should be considered as a part of the specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values.

[0026] In addition, it should be noted that the use of words such as "first" and "second" to limit the activated culture medium, liquid seed culture medium, and solid culture medium is only to facilitate the distinction between the culture medium formulas used for the two bacteria. Unless otherwise stated, the above words have no special meaning and therefore cannot be understood as limiting the scope of protection of the present invention.

[0027] The experimental methods in the following examples without specifying specific conditions are usually measured according to national standards; if there is no corresponding national standard, it is carried out according to the general international standards or the standard requirements proposed by relevant enterprises. Unless otherwise specified, all parts are by weight and all percentages are by weight.

[0028] The jelly-like Bacillus and Bacillus subtilis used in the present invention are provided by Shanghai Green Joy Biological Co., Ltd.; and Streptomyces griseorhizoides is provided by the laboratory of Shanghai Jiaotong University.

[0029] Example 1

[0030] The invention discloses a highly efficient and clean organic material decomposing bacterial agent, which is composed of 40% of gelatinous bacillus powder and 60% of griseorhizium streptomyces powder.

[0031] Preparation of jelly-like Bacillus powder:

[0032] (1) inoculating a loop of jelly-like Bacillus on 20 mL of the first activation culture medium, and performing activation culture in a constant temperature dark incubator at 30° C. for 2 days to obtain a jelly-like Bacillus bacterial liquid, wherein the formula of the first activation culture medium is 20.0 g of glucose, 16.7 g of peptone, 5.3 g of NaCl, 5.3 g of beef extract, and 15 g of agar per liter of distilled water, and the pH is 7.0;

[0033] (2) placing 1 mL of the jelly-like Bacillus bacterial liquid into 200 mL of a sterilized first liquid seed culture medium, and culturing the culture in a shaking table at 30° C. and 200 rpm for 2 days to obtain a jelly-like Bacillus fermentation liquid, wherein the formula of the first liquid seed culture medium is 20.0 g of glucose, 16.7 g of peptone, 5.3 g of NaCl, and 5.3 g of beef extract per liter of distilled water, and the pH value is 7.0;

[0034] (3) 150 mL of the fermentation liquid was inoculated on 1 kg of the first solid culture medium for fermentation culture. The first solid culture medium was composed of 90% bran, 9.8% rice bran, 0.05% MgSO4·7H2O, 0.05% NH4SO4, and 0.1% CaCO3 in mass percentage, with a pH of 7.0. Water was added in equal weight and mixed, and the mixture was sterilized at 121° C. for 1 h before use. The mixture was fermented at a constant temperature of 32° C. for 7 days. At the end of the fermentation culture, the moisture content was 30% and the number of effective viable bacteria was 300 million / g. The fermented cells of the jelly-like Bacillus and their products were collected and crushed to obtain a jelly-like Bacillus powder.

[0035] Preparation of Streptomyces griseorrhiza powder:

[0036] (1) Inoculate two rings of Streptomyces griseus on 20 mL of the second activation medium, place the culture medium in a 32° C. constant temperature dark incubator for 4 days to activate and obtain a Streptomyces griseus bacterial solution, wherein the formula of the second activation medium is 20.0 g of soluble starch, 1.0 g of KNO3, 0.5 g of K2HPO4, 0.5 g of MgSO4·7H2O, 0.5 g of NaCl, and 15 g of agar per liter of distilled water, and the pH value is 7.2;

[0037] (2) inoculating 5 mL of the bacterial solution into 200 mL of a second liquid seed culture medium, and performing shaking culture at 32° C. and 200 rpm for 5 days to obtain a Streptomyces griseus seed culture medium, wherein the formula of the second liquid seed culture medium is 20.0 g of soluble starch, 1.0 g of KNO3, 0.5 g of K2HPO4, 0.5 g of MgSO4·7H2O, and 0.5 g of NaCl per liter of distilled water, and the pH value is 7.2;

[0038] (3) inoculating 200 mL of the seed liquid onto 1 kg of a second solid culture medium for fermentation culture, wherein the second solid culture medium is composed of 99% rice bran, 0.1% KNO3, 0.05% K2HPO4, 0.05% MgSO4·7H2O, 0.05% NaCl, and 0.75% CaCO3 by mass percentage, with a pH of 7.2, adding water in equal weight and mixing, sterilizing at 121° C. for 1 h before use, and fermenting at a constant temperature of 35° C. for 5 days. At the end of the fermentation culture, the moisture content is 15% and the number of effective viable bacteria is 500 million / g. The fermented cells of Streptomyces griseus and their products are collected, crushed, and Streptomyces griseus powder is obtained.

[0039] Example 2

[0040] The invention discloses an efficient and clean organic material decomposing bacterial agent, which is composed of 50% of gelatinous bacillus powder and 50% of griseorhizium streptomyces powder.

[0041] The preparation methods of the jelly-like Bacillus powder and the griseo-red Streptomyces powder are the same as those in Example 1.

[0042] Example 3

[0043] The invention discloses a highly efficient and clean organic material decomposing bacterial agent, which is composed of 60% of gelatinous bacillus powder and 40% of griseorhizium streptomyces powder.

[0044] The preparation methods of the jelly-like Bacillus powder and the griseo-red Streptomyces powder are the same as those in Example 1.

[0045] Comparative Example 1

[0046] The organic material decomposing bacteria in this comparative example are 40% of Bacillus subtilis powder and 60% of Streptomyces griseus powder.

[0047] The preparation method of Bacillus subtilis powder is the same as the preparation method of jelly-like Bacillus powder.

[0048] Comparative Example 2

[0049] The organic material decomposing bacteria in this comparative example are 20% of Bacillus subtilis powder, 20% of Bacillus gelatinosa powder and 60% of Streptomyces griseus powder.

[0050] Comparative Example 3

[0051] The organic material decomposing bacteria agent in this comparative example is 60% of Bacillus subtilis powder and 40% of Bacillus gelatinous powder.

[0052] Comparative Example 4

[0053] The organic material decomposing bacteria in this comparative example are 30% of Bacillus jelly-like powder and 70% of Streptomyces griseus powder.

[0054] Comparative Example 5

[0055] The organic material decomposing bacteria in this comparative example are 70% of Bacillus jelly-like powder and 30% of Streptomyces griseus powder.

[0056] Example 4

[0057] The corn stalks are collected and crushed into small pieces of 5-6 cm in length.

[0058] The experiment was carried out in Chongming District, Shanghai. The following six treatment areas were set up, arranged in random blocks, repeated three times, and the area of ​​the plot was 300m 2 .

[0059] Treatment 1: Prepare multiple landfill bags that are evenly permeable to water, put 2000g of straw into each landfill bag, landfill to a depth of 10cm, cover with soil evenly, mark them, and keep the distance between bags 50cm.

[0060] Treatment 2: Prepare multiple uniformly permeable landfill bags, and put 2000 g of uniformly mixed straw, urea and inactivated organic material decomposing bacteria agent in each landfill bag (the mass ratio of straw, urea and decomposing bacteria agent is 500:2:5), and the subsequent operation is the same as treatment 1. Inactivated organic material decomposing bacteria agent (the formula is the same as that of Example 1, and the inactivation condition is to inactivate the organic material decomposing bacteria agent by medical high pressure and high temperature sterilization method).

[0061] Treatment 3: Prepare multiple landfill bags that are uniformly permeable to water, and put 2000 g of uniformly mixed straw, urea and the organic material decomposing agent of Example 1 into each landfill bag (the proportion of the three is the same as that of Treatment 2), and the subsequent operations are the same as those of Treatment 1.

[0062] Treatment 4: Example 2 organic material decomposition bacteria agent + straw + urea, subsequent operations are the same as treatment 3.

[0063] Treatment 5: Example 3 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0064] Treatment 6: Comparative Example 1 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0065] Treatment 7: Comparative Example 2 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0066] Treatment 8: Comparative Example 3 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0067] Treatment 9: Comparative Example 4 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0068] Treatment 10: Comparative Example 5 organic material decomposing bacteria agent + straw + urea, subsequent operations are the same as Treatment 3.

[0069] The physical conditions of the soil in the test land are shown in Table 1.

[0070] Table 1 Physical conditions of soil in test land

[0071] Total nitrogen (%) Available phosphorus mg / kg Available potassium mg / kg Organic matter g / kg pH soil 0.132 15.4 121 15.6 6.9

[0072] Take out a landfill bag every 10 days, separate the dirt, weeds, gravel and other debris, and make the landfill bag water-permeable. Measure the maturity of the straw, including its color, smell, weight loss rate, tension and feel.

[0073] (1) The evaluation of the appearance, color, smell and feel of corn stalks after different treatments is shown in Table 2.

[0074] Table 2 Evaluation of corn stalk appearance, color, smell and feel under different treatments

[0075]

[0076] As shown in Table 2, different treatments have an impact on the appearance, color, smell and feel of corn stalks. Treatment 3 has the most obvious changes, with the color changing from yellowish-brown-yellow to dark yellow in 10d-30d, the smell changing from light rotten smell to rotten smell to black stinky smell, and the feel changing from soft-soft to soft-soft and smooth; Treatments 4 and 5 are second, with the color changing from medium yellow-yellow to brown-yellow in 10d-30d, the smell changing from light rotten smell to rotten smell to rotten smell, and the feel changing from soft-soft to soft; the color changes, smell and feel changes of other treatments from 10d-30d are worse than those of treatments 3, 4 and 5.

[0077] (2) The effects of different treatments on the weight loss rate of corn straw are shown in Table 3.

[0078] Table 3 Effects of different treatments on weight loss rate of corn stalks

[0079] Bag Buried Days 10d(％) 20d(％) 30d(％) Process 1 11.06 20.10 48.50 Process 2 11.57 25.24 50.61 Process 3 20.33 33.76 62.76 Process 4 16.02 29.06 55.06 Process 5 14.41 28.40 53.40 Process 6 11.56 26.56 47.56 Process 7 11.74 27.19 45.69 Process 8 11.94 26.95 46.94 Process 9 12.63 27.64 47.53 Processing 10 11.42 26.17 44.25

[0080] As shown in Table 3, on the 10th day, the weight loss rate of treatment 3 was the highest at 20.33%, followed by treatments 4 and 5, and the weight loss rate of treatment 1 was the lowest at 11.06%; from 10th to 30th day, the weight loss rate of corn stalks in each treatment showed a trend of gradual increase; on the 30th day, the weight loss rate of corn stalks in treatment 3 was the highest at 62.76%, followed by treatments 4 and 5, which were 55.06% and 53.40%, respectively; the other treatments were all lower than treatment 5. Among them, treatment 7 was a combination of Bacillus subtilis powder, Bacillus jelly powder and Streptomyces griseus powder, and its effect on the weight loss rate of corn stalks was not as good as that of treatment 6. The possible reason was that Bacillus subtilis and Bacillus jelly may produce antagonism.

[0081] (3) The effects of different treatments on the pulling force of corn stalks are shown in Table 4.

[0082] Table 4 Effects of different treatments on the pulling force of corn stalks

[0083] Bag Buried Days 10d(N) 20d(N) 30d(N) Process 1 35.68 27.19 18.35 Process 2 33.17 23.26 16.23 Process 3 30.82 16.47 9.39 Process 4 31.54 21.51 12.79 Process 5 32.28 24.25 13.25 Process 6 34.60 28.51 16.57 Process 7 33.32 27.97 16.25 Process 8 36.28 29.11 22.29 Process 9 34.85 25.18 15.37 Processing 10 33.77 25.38 18.68

[0084] As shown in Table 4, different treatments have obvious changes on the pulling force of corn straw. With the extension of time, the pulling force of the straw applied with organic material decomposition bacteria agent decreases rapidly. Treatment 3 decreases the fastest within 10d-30d, decreasing by 69.53%; Treatment 4 decreases by 59.45%, Treatment 5 decreases by 58.95%, and the decrease range of other treatments is 38.56%-55.90%.

[0085] In summary, the organic material decomposing bacteria agent with a mass ratio of Bacillus gelatinosa powder (40%-60%) and Streptomyces griseus powder (60%-40%) has a better decomposition effect, indicating that the combination effect within the scope of the present invention is better than that of the comparative example.

[0086] Example 5

[0087] Experiment on Returning Corn Straw to Field and Planting Wheat

[0088] According to treatments 1 to 10 in Example 4, corn stalks were first decomposed for at least one week, and then wheat seeds were sown on the soil buried with the decomposed stalks, and conventional fertilizers were applied during the planting process. The effects of the above different treatments on the biological traits and yield of wheat are shown in Table 5.

[0089] Table 5 Effects of different treatments on wheat biological traits and yield

[0090]

[0091]

[0092] As shown in Table 5, the biological traits of wheat in treatments 3, 4 and 5 were good. The yield per mu of treatment 3 was 366.96 kg, the yield per mu of treatment 4 was 357.74 kg, and the yield per mu of treatment 3 was 359.30 kg. The yield per mu of treatments 3 to 5 increased by 9.72%-12.58% compared with treatment 1; the yield of the other treatments increased by 1.4%-6.99% compared with treatment 1.

[0093] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A highly efficient and clean organic material decomposition agent, It is characterized in that It is composed of jelly-like Bacillus powder and gray-slightly red Streptomyces powder, and is used for the composting treatment of organic materials; The Streptomyces griseorhizium powder accounts for 40% to 60% of the total mass of the organic material decomposing bacteria agent; Preparation of jelly-like Bacillus powder: (1) inoculating a loop of jelly-like Bacillus on 20 mL of the first activation culture medium, and performing activation culture in a constant temperature dark incubator at 30° C. for 2 days to obtain a jelly-like Bacillus bacterial liquid, wherein the formula of the first activation culture medium is 20.0 g of glucose, 16.7 g of peptone, 5.3 g of NaCl, 5.3 g of beef extract, and 15 g of agar per liter of distilled water, and the pH is 7.0; (2) placing 1 mL of the jelly-like Bacillus bacterial liquid into 200 mL of a sterilized first liquid seed culture medium, and culturing the culture in a shaking table at 30° C. and 200 rpm for 2 days to obtain a jelly-like Bacillus fermentation liquid, wherein the formula of the first liquid seed culture medium is 20.0 g of glucose, 16.7 g of peptone, 5.3 g of NaCl, and 5.3 g of beef extract per liter of distilled water, and the pH value is 7.0; (3) 150 mL of the fermentation liquid was inoculated into 1 kg of the first solid culture medium for fermentation culture, wherein the first solid culture medium was composed of 90% bran, 9.8% rice bran, and MgSO 4 7H 2 O 0.05%、NH 4 SO 4 0.05%, CaCO 3 0.1%, pH = 7.0, add water according to equal weight, mix well, sterilize at 121°C for 1h before use, and ferment at 32°C for 7d. At the end of fermentation culture, the moisture content is 30% and the effective viable bacteria count is 300 million / g. The fermented cells of jelly-like Bacillus and their products are collected and crushed to obtain jelly-like Bacillus powder; Preparation of Streptomyces griseorrhiza powder: (1) Two rings of Streptomyces griseus were inoculated on 20 mL of the second activation medium and cultured in a dark incubator at 32° C. for 4 days to activate and obtain a Streptomyces griseus bacterial solution. The formula of the second activation medium was 20.0 g of soluble starch, KNO, and 10% ethanol per liter of distilled water. 3 1.0g, K 2 HPO 4 0.5 g, MgSO 4 7H 2 O 0.5g, NaCl 0.5g, agar 15g, pH = 7.2; (2) 5 mL of the bacterial solution was inoculated into 200 mL of the second liquid seed culture medium, and the culture was carried out in a shaking incubator at 32° C. and 200 rpm for 5 days to obtain a seed solution of Streptomyces griseus. The formula of the second liquid seed culture medium was 20.0 g of soluble starch, KNO, and 1.3 g of ethanol per liter of distilled water. 3 1.0g, K 2 HPO 4 0.5 g, MgSO 4 7H 2 O 0.5g, NaCl 0.5g, pH=7.2; (3) Inoculate 200 mL of the seed solution onto 1 kg of a second solid culture medium for fermentation culture, wherein the second solid culture medium comprises 99% rice bran, KNO 3 0.1%、K 2 HPO 4 0.05%, MgSO4·7H 2 O 0.05%, NaCl 0.05%, CaCO 3 0.75%, pH = 7.2, add water according to equal weight, mix well, sterilize at 121 ° C for 1 hour before use, and ferment at 35 ° C for 5 days. At the end of fermentation culture, the moisture content is 15% and the effective viable count is 500 million / g. The fermented cells of Streptomyces griseus and its products are collected and crushed to obtain Streptomyces griseus powder.

2. According to claim 1, a highly efficient and clean organic material decomposition agent, It is characterized in that The Streptomyces griseus powder accounts for 60% of the total mass of the organic material decomposing bacteria agent.

3. The method for preparing the highly efficient and clean organic material decomposing agent according to any one of claims 1 to 2, It is characterized in that The method comprises the following steps: preparing fermented bacteria of jelly-like bacillus and streptomyces griseus and their products respectively by solid-state fermentation method, obtaining jelly-like bacillus powder and streptomyces griseus powder after drying and crushing, mixing the two powders evenly to obtain organic material decomposing bacteria agent, and storing the mixture in a cool and dry place.

4. The use of the highly efficient and clean organic material decomposing bacteria agent according to any one of claims 1 to 3, It is characterized in that After the uniformly mixed organic materials, urea and organic material decomposing bacteria are buried, the soil is evenly covered and water is irrigated. After the organic materials are decomposed for at least one week, wheat seeds can be sown on the soil buried with the decomposed organic materials for planting wheat. The mass ratio of the organic materials, the decomposing bacteria and the urea is 250:1:2.5.

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