A kind of production method of black skinned Alpinia albuminata

Abstract

The invention discloses a method for producing black-skinned Alpinia thunbergii, and relates to the technical field of black-skinned Alpinia thunbergii. The method comprises the following steps: step 1: preparation of mother species; step 2: preparation of original species; step 3: preparation of production species; step 4: artificial cultivation; and step 5: soil covering cultivation. The invention performs spore separation or tissue separation on the fruiting body of black-skinned Alpinia thunbergii under sterile conditions to separate the fruiting body mycelium, transfers the fruiting body mycelium to a test tube filled with a culture medium to obtain the mother species, then connects the mother species to the original species culture medium to cultivate the mother species, and finally cultivates the production species through the production species culture medium, then, the production species culture medium is placed in a culture plastic bag for sterilization, cooled after the sterilization is completed, the prepared original species is inoculated to obtain a mushroom stick, the mushroom stick is placed in a culture room for cultivation, and the culture is cultured until the color changes, indicating that the physiological maturity can be transferred to the soil covering cultivation stage, thereby facilitating the artificial cultivation of black-skinned Alpinia thunbergii.

Description

Technical Field

[0001] The invention relates to the technical field of black-skinned Alpinia terrestris, and in particular to a production method of black-skinned Alpinia terrestris. Background Art

[0002] Black-skinned Alpinia albuminata belongs to the Basidiomycetes, Hymenomycetes, Agaricales, Trichoderma, also known as long-rooted mushroom. This fungus has edible and medicinal value, and is also one of the traditional medicinal fungi. It has attracted much attention because of its fresh and mellow fragrance, tender meat, white jade, unique taste, edible raw or cooked, dual-purpose food and medicine, and rich nutrition.

[0003] The existing technology has the following problems:

[0004] In its natural state, the black-skinned Alpinia tremella has extremely stringent requirements for the growth environment, and it is not easy to achieve artificial batch cultivation. In addition, the existing cultivation devices lack effective sealing when in use, and lack effective fixation for the culture racks. As a result, when the cultivation box shakes due to external influences, the culture racks are easy to slide, affecting the cultivation of the black-skinned Alpinia tremella. Summary of the invention

[0005] The invention provides a method for producing black-skinned Alpinia terrestris to solve the problems raised in the above background technology.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A method for producing black-skinned Alpinia tremella, comprising the following steps:

[0008] Step 1: Preparation of mother stock;

[0009] Step 2: Preparation of stock;

[0010] Step 3: Preparation of production seeds;

[0011] Step 4: Artificial cultivation;

[0012] Step 5: Cover with soil and cultivate.

[0013] A further improvement of the technical solution of the present invention is that the step 1 further includes the following steps:

[0014] A1: Under sterile conditions, spore separation or tissue separation is performed on the fruiting body of Alternaria japonica to obtain the fruiting body mycelium; tissue separation is performed on the rhizoid of Alternaria japonica to obtain the rhizoid mycelium;

[0015] A2: Transfer the fruiting body mycelium into a test tube filled with culture medium and culture at 22-25°C. When the mycelium grows to 1.3-1.8 cm, inoculate the rhizoid mycelium and continue to culture for 5-8 days to obtain mature mother strains.

[0016] A3: The culture medium is: 1000 ml of wheat straw juice, 10-15 g of agar, and 25 g of glucose.

[0017] A further improvement of the technical solution of the present invention is that the step 2 further includes the following steps:

[0018] B1: After the mother seed is cultured, it is inoculated into the original seed culture medium and matures after 20-25 days;

[0019] B2: The composition of the original seed culture medium is: 65% wheat straw, 32% cottonseed hull, 3% gypsum powder, and a water content of 60% to 65%.

[0020] A further improvement of the technical solution of the present invention is that the step three further includes the following steps:

[0021] C1: Transfer the mycelium to a test tube containing production medium for cultivation;

[0022] C2: The production culture medium is: 67% wheat straw, 23% cottonseed hull, 7% bran, 3% gypsum powder, and the water content is 65% to 70%.

[0023] A further improvement of the technical solution of the present invention is that the step 4 further includes the following steps:

[0024] D1: Sterilize the production culture medium in a culture plastic bag. During the sterilization process, high pressure sterilization is selected at a pressure of 1.5 kg / cm2 for 2.53 hours, followed by cooling.

[0025] D2: inoculating the prepared original seed to obtain bacterial sticks;

[0026] D3: Place the bacterial sticks in the culture room and culture them at a temperature of 15-23°C for 10 to 15 days.

[0027] A further improvement of the technical solution of the present invention is that it includes an incubator, the top of which is fixedly connected to a temperature control device, the inner wall of which is fixedly connected to a fixing frame, the middle of the temperature control device is movably connected to a culture frame, the top of the culture frame is movably connected to a culture tank, and the front end of the incubator is fixedly connected to a sealing mechanism.

[0028] A further improvement of the technical solution of the present invention is that: the fixed frame includes a fixed frame, the fixed frame is fixedly connected to the inside of the incubator, the inner side of the fixed frame is movably connected with a card block, the top of the card block is fixedly connected with a tightening spring, the middle part of the fixed frame is slidably connected with a sliding plate, the right side of the sliding plate is movably connected with a rotating rod, the rotating rod is movably connected to the inside of the fixed frame, one end of the rotating rod is movably connected with a card rod, a slot is provided on the side of the card block, and a reset mechanism is provided on the side of the card rod.

[0029] A further improvement of the technical solution of the present invention is that the reset mechanism includes a movable plate, the movable plate is movably connected to the inner side of the fixed frame, the bottom end of the movable plate is fixedly connected to a top block, the top end of the movable plate is fixedly connected to an inclined sliding block, the top of the inclined sliding block is fixedly connected to a reset spring, the top of the reset spring is fixedly connected to the inner wall of the fixed frame, the bottom side of the inclined sliding block is movably connected to a connecting sliding block, and the connecting sliding block is fixedly connected to the side of the clamping rod.

[0030] A further improvement of the technical solution of the present invention is that: the sealing mechanism includes a connecting plate, the connecting plate is fixedly connected to the front end of the incubator, the side of the connecting plate is fixedly connected with a sealing gasket, the middle of the sealing gasket is movably connected with a driving plate, the bottom end of the driving plate is fixedly connected with an extrusion spring, one end of the extrusion spring is fixedly connected to the side of the connecting plate, the bottom end of the driving plate is slidably connected with a guide rod, the guide rod is fixedly connected to the side of the connecting plate, both sides of the driving plate are movably connected with pull rods, the bottom end of the pull rod is fixedly connected with a pull plate, the side of the pull plate is movably connected with a push rod, the top end of the push rod is movably connected with an extrusion plate, and the extrusion plate is fixedly connected to the inner side of the sealing gasket.

[0031] Due to the adoption of the above technical solution, the present invention has the following technical advances compared with the prior art:

[0032] 1. The present invention provides a method for producing black-skinned Alpinia chorii. The method comprises the following steps: performing spore separation or tissue separation on a fruiting body of the black-skinned Alpinia chorii under aseptic conditions to separate a fruiting body mycelium, transferring the fruiting body mycelium into a test tube filled with a culture medium to obtain a mother seed, then accessing the mother seed to cultivate the mother seed in an original seed culture medium, and finally cultivating the production seed in a production seed culture medium. After that, the production seed culture medium is placed in a culture plastic bag for sterilization, and after the sterilization is completed, the production seed is cooled, and the prepared original seed is inoculated to obtain a mushroom stick, and the mushroom stick is placed in a culture room for cultivation, and the mushroom stick is cultivated until the color changes, indicating that the physiological maturity can be transferred to the soil covering cultivation stage, thereby facilitating the artificial cultivation of the black-skinned Alpinia chorii.

[0033] 2. The present invention provides a method for producing black-skinned Alpinia terrestris. Through the mutual cooperation among a fixed frame, a card block, a tightening spring, a sliding plate, a rotating rod, a card rod, a slot, and a reset mechanism, the card block is pushed by the tightening spring so that the card block is stuck on the side of a culture rack, thereby fixing the culture rack, and by pushing the culture rack, the sliding plate can be made to slide, and the card rod is driven by the rotating rod to be stuck in the inside of the slot, thereby fixing the card block, facilitating the removal of the culture rack, facilitating the use of the culture rack, and preventing the culture rack from slipping.

[0034] 3. The present invention provides a method for producing black-skinned Alpinia terrestris. Through the mutual cooperation among a connecting plate, a sealing gasket, a driving plate, an extrusion spring, a guide rod, a pull rod, a pull plate, a push rod, and an extrusion plate, when the door of the incubator is closed, the sealing gasket is squeezed to seal the incubator, and the driving plate can be pushed to move, so that the pull plate can be pulled to move by the pull rod, and the extrusion plate can be pushed by the push rod, and the sealing gasket is squeezed, so that the sealing effect of the incubator is better, and the cultivation of black-skinned Alpinia terrestris is convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the production process of the present invention;

[0036] Figure 2 It is a structural schematic diagram of the incubator of the present invention;

[0037] Figure 3 is a schematic cross-sectional structure diagram of a fixing frame of the present invention;

[0038] Figure 4 It is a schematic cross-sectional structure diagram of the reset mechanism of the present invention;

[0039] Figure 5 It is a schematic cross-sectional structure diagram of the sealing mechanism of the present invention.

[0040] In the figure: 1. incubator; 2. temperature control device; 3. fixed frame; 31. fixed frame; 32. clamping block; 33. tightening spring; 34. sliding plate; 35. rotating rod; 36. clamping rod; 37. slot; 38. reset mechanism; 381. moving plate; 382. top block; 383. inclined slider; 384. reset spring; 385. connecting slider; 4. incubator; 5. incubator; 6. sealing mechanism; 61. connecting plate; 62. sealing pad; 63. driving plate; 64. extrusion spring; 65. guide rod; 66. pull rod; 67. pull plate; 68. push rod; 69. extrusion plate. DETAILED DESCRIPTION

[0041] The present invention is further described in detail below in conjunction with embodiments:

[0042] Example 1

[0043] like Figure 1-5 As shown, the present invention provides a method for producing black-skinned Alpinia tremella, and the method for producing black-skinned Alpinia tremella comprises the following steps:

[0044] Step 1: Preparation of mother stock;

[0045] Step 2: Preparation of stock;

[0046] Step 3: Preparation of production seeds;

[0047] Step 4: Artificial cultivation;

[0048] Step 5: Cover with soil and cultivate.

[0049] Step one also includes the following steps:

[0050] A1: Under sterile conditions, spore separation or tissue separation is performed on the fruiting body of Alternaria japonica to obtain the fruiting body mycelium; tissue separation is performed on the rhizoid of Alternaria japonica to obtain the rhizoid mycelium;

[0051] A2: Transfer the fruiting body mycelium into a test tube filled with culture medium and culture at 22-25°C. When the mycelium grows to 1.3-1.8 cm, inoculate the rhizoid mycelium and continue to culture for 5-8 days to obtain mature mother strains.

[0052] A3: The culture medium is: 1000 ml of wheat straw juice, 10-15 g of agar, and 25 g of glucose.

[0053] Step 2 also includes the following steps:

[0054] B1: After the mother seed is cultured, it is inoculated into the original seed culture medium and matures after 20-25 days;

[0055] B2: The composition of the original seed culture medium is: 65% wheat straw, 32% cottonseed hull, 3% gypsum powder, and a water content of 60% to 65%.

[0056] Step three also includes the following steps:

[0057] C1: Transfer the mycelium to a test tube containing production medium for cultivation;

[0058] C2: The production culture medium is: 67% wheat straw, 23% cottonseed hull, 7% bran, 3% gypsum powder, and the water content is 65% to 70%.

[0059] Step 4 also includes the following steps:

[0060] D1: Sterilize the production culture medium in a culture plastic bag. During the sterilization process, high pressure sterilization is selected at a pressure of 1.5 kg / cm2 for 2.53 hours, followed by cooling.

[0061] D2: inoculating the prepared original seed to obtain bacterial sticks;

[0062] D3: Place the bacterial sticks in the culture room and culture them at a temperature of 15-23°C for 10 to 15 days.

[0063] In this embodiment, spore separation or tissue separation is performed on the fruiting body of black-skinned albuminus under sterile conditions to separate the fruiting body mycelium, the fruiting body mycelium is transferred to a test tube filled with a culture medium to obtain a mother species, and then the mother species is connected to the original species culture medium to cultivate the mother species, and finally the production species is cultivated through the production species culture medium, and then the production species culture medium is placed in a culture plastic bag for sterilization, and after the sterilization is completed, it is cooled, and the prepared original species is inoculated to obtain a mushroom stick, and the mushroom stick is placed in a culture room for cultivation, and the culture is cultured until the color changes, indicating that the physiological maturity can be transferred to the soil covering cultivation stage, which is convenient for the artificial cultivation of black-skinned albuminus.

[0064] Example 2

[0065] like Figure 1-5 As shown, on the basis of Example 1, the present invention provides a technical solution: preferably, it includes an incubator 1, the top of the incubator 1 is fixedly connected with a temperature control device 2, the inner wall of the incubator 1 is fixedly connected with a fixing frame 3, the middle of the temperature control device 2 is movably connected with a culture frame 4, the top of the culture frame 4 is movably connected with a culture tank 5, the front end of the incubator 1 is fixedly connected with a sealing mechanism 6, the fixing frame 3 includes a fixing frame 31, the fixing frame 31 is fixedly connected to the inside of the incubator 1, the inner side of the fixing frame 31 is movably connected with a clamping block 32, the top of the clamping block 32 is fixedly connected with a tightening spring 33, the middle of the fixing frame 31 is slidably connected with a sliding plate 34, the right side of the sliding plate 34 is movably connected with a rotating rod 35, the rotating rod 35 is movably connected to the inside of the fixing frame 31, one end of the rotating rod 35 is movably connected with a clamping rod 36, a side of the clamping block 32 is provided with a slot 37, and a side of the clamping rod 36 is provided with a reset mechanism 38.

[0066] In this embodiment, when cultivating black-skinned Alpinia fasciata, the mycelium is placed in the cultivation tank 5 on the cultivation rack 4 and placed in the cultivation box 1. The clamping block 32 is pushed by the tightening spring 33 so that the clamping block 32 is stuck on the side of the cultivation rack 4, thereby fixing the cultivation rack 4. When the box door is closed, the cultivation box 1 can be sealed by the sealing mechanism 6, and the temperature in the cultivation box 1 is controlled by the temperature control device 2, so that the black-skinned Alpinia fasciata can be cultivated. After the cultivation is completed, the sliding plate 34 can be made to slide by pushing the cultivation rack 4, and the clamping rod 36 is driven by the rotating rod 35 to be stuck in the inside of the slot 37, thereby fixing the clamping block 32, facilitating the removal of the cultivation rack 4 and the cultivation tank 5.

[0067] Example 3

[0068] like Figure 1-5As shown, on the basis of Example 2, the present invention provides a technical solution: preferably, the reset mechanism 38 includes a movable plate 381, the movable plate 381 is movably connected to the inner side of the fixed frame 31, the bottom end of the movable plate 381 is fixedly connected with a top block 382, ​​the top of the movable plate 381 is fixedly connected with an inclined sliding block 383, the top of the inclined sliding block 383 is fixedly connected with a reset spring 384, the top of the reset spring 384 is fixedly connected to the inner wall of the fixed frame 31, the bottom side of the inclined sliding block 383 is movably connected with a connecting sliding block 385, and the connecting sliding block 385 is fixedly connected to the side of the clamping rod 36.

[0069] In this embodiment, the block 32 is fixed by the clamping rod 36 to facilitate the removal of the culture rack 4, which will remove the restriction on the top block 382. Therefore, under the pushing of the reset spring 384, the inclined sliding block 383 can be pushed to move, and the movable plate 381 and the top block 382 can be pushed out. At the same time, the connecting sliding block 385 can be pushed to move by the inclined sliding block 383, which facilitates the resetting of the clamping rod 36.

[0070] Example 4

[0071] like Figure 1-5 As shown, on the basis of Example 2, the present invention provides a technical solution: preferably, the sealing mechanism 6 includes a connecting plate 61, the connecting plate 61 is fixedly connected to the front end of the incubator 1, the side of the connecting plate 61 is fixedly connected with a sealing gasket 62, the middle of the sealing gasket 62 is movably connected with a driving plate 63, the bottom end of the driving plate 63 is fixedly connected with an extrusion spring 64, one end of the extrusion spring 64 is fixedly connected to the side of the connecting plate 61, the bottom end of the driving plate 63 is slidably connected with a guide rod 65, the guide rod 65 is fixedly connected to the side of the connecting plate 61, both sides of the driving plate 63 are movably connected with pull rods 66, the bottom end of the pull rod 66 is fixedly connected with a pull plate 67, the side of the pull plate 67 is movably connected with a push rod 68, the top of the push rod 68 is movably connected with an extrusion plate 69, and the extrusion plate 69 is fixedly connected to the inner side of the sealing gasket 62.

[0072] In this embodiment, when the door of the incubator 1 is closed, the sealing gasket 62 is squeezed to seal the incubator 1, and the driving plate 63 can be pushed to move along the guide rod 65, and the extrusion spring 64 can be squeezed, so that the pull plate 67 can be pulled to move by the pull rod 66, and the extrusion plate 69 can be pushed by the push rod 68, and the sealing gasket 62 can be squeezed, so that the sealing effect of the incubator 1 is better, and the cultivation of black-skinned chicken mushroom is convenient.

[0073] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not depart from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A method for producing black-skinned Alpinia hortensis, characterized in that: The method for producing black-skinned Alpinia thunbergii comprises the following steps: Step 1: Preparation of mother stock; Step 2: Preparation of stock; Step 3: Preparation of production seeds; Step 4: Artificial cultivation; Step 5: Covering with soil for cultivation; The step 1 also includes the following steps: A1: Perform spore separation or tissue separation on the fruiting body of Alpinia cerana under sterile conditions to obtain the fruiting body mycelium; A2: Transfer the fruiting body mycelium into a test tube filled with culture medium and culture at 22-25°C. When the mycelium grows to 1.3-1.8 cm, inoculate the rhizoid mycelium and continue to culture for 5-8 days to obtain mature mother strains. A3: The culture medium is: 1000 ml of wheat straw juice, 10-15 g of agar, and 25 g of glucose; The step 2 also includes the following steps: B1: After the mother seed is cultured, it is inoculated into the original seed culture medium and matures after 20-25 days; B2: The composition of the original seed culture medium is: 65% wheat straw, 32% cottonseed hull, 3% gypsum powder, and a water content of 60% to 65%; The step three also includes the following steps: C1: Transfer the mycelium to a test tube containing production medium for cultivation; C2: The production culture medium is: 67% wheat straw, 23% cottonseed hull, 7% bran, 3% gypsum powder, and the water content is 65% to 70%; The step 4 also includes the following steps: D1: Sterilize the production culture medium in a culture plastic bag. During the sterilization process, high pressure sterilization is selected at a pressure of 1.5 kg / cm2 for 2.53 hours, followed by cooling. D2: inoculating the prepared original seed to obtain bacterial sticks; D3: Place the bacterial sticks in the culture room for cultivation at a temperature of 15-23°C for 10 to 15 days; The cultivation device comprises a cultivation box (1), the top of the cultivation box (1) is fixedly connected to a temperature control device (2), the inner wall of the cultivation box (1) is fixedly connected to a fixing frame (3), the middle of the temperature control device (2) is movably connected to a cultivation frame (4), the top of the cultivation frame (4) is movably connected to a cultivation tank (5), the front end of the cultivation box (1) is fixedly connected to a sealing mechanism (6), the fixing frame (3) comprises a fixing frame (31), the fixing frame (31) is fixedly connected to the inside of the cultivation box (1), the inner side of the fixing frame (31) is movably connected to a clamping block (32), the top of the clamping block (32) is fixedly connected to a tightening spring (33), and the sealing mechanism (6) is fixedly connected to the front end of the cultivation box (1). The middle part of the fixed frame (31) is slidably connected to a sliding plate (34), the right side of the sliding plate (34) is movably connected to a rotating rod (35), the rotating rod (35) is movably connected to the inside of the fixed frame (31), one end of the rotating rod (35) is movably connected to a clamping rod (36), a slot (37) is provided on the side of the clamping block (32), a reset mechanism (38) is provided on the side of the clamping rod (36), the reset mechanism (38) comprises a moving plate (381), the moving plate (381) is movably connected to the inside of the fixed frame (31), the bottom end of the moving plate (381) is fixedly connected to a top block (382), the moving plate (381) ) is fixedly connected to the top of an inclined slider (383), the top of the inclined slider (383) is fixedly connected to a return spring (384), the top of the return spring (384) is fixedly connected to the inner wall of the fixed frame (31), the bottom side of the inclined slider (383) is movably connected to a connecting slider (385), the connecting slider (385) is fixedly connected to the side of the clamping rod (36), the sealing mechanism (6) comprises a connecting plate (61), the connecting plate (61) is fixedly connected to the front end of the incubator (1), the side of the connecting plate (61) is fixedly connected to a sealing gasket (62), and the middle part of the sealing gasket (62) is movably connected to a driving plate (63), The bottom end of the driving plate (63) is fixedly connected to an extrusion spring (64), one end of which is fixedly connected to the side of the connecting plate (61), the bottom end of the driving plate (63) is slidably connected to a guide rod (65), the guide rod (65) is fixedly connected to the side of the connecting plate (61), the two sides of the driving plate (63) are movably connected to pull rods (66), the bottom end of the pull rod (66) is fixedly connected to a pull plate (67), the side of the pull plate (67) is movably connected to a push rod (68), the top end of the push rod (68) is movably connected to an extrusion plate (69), and the extrusion plate (69) is fixedly connected to the inner side of the sealing gasket (62).

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