Oil-based composition and application thereof, probiotic drops and preparation method thereof

Abstract

The present invention provides an oil-based composition and its application, probiotic drops and its preparation method, wherein the oil-based composition includes medium-chain triglycerides, DHA algae oil and walnut oil in a mass ratio of (3-4): (3-4): (1-3); (3-3.8): (3-3.8): (1-2.4), more preferably 3: 3: (1-2); by using three kinds of fats and oils in a mass ratio within the above range to form the oil-based composition, the oil-based composition has good oil stability, can reduce or even avoid probiotics from settling therein, and can increase the dispersion speed of probiotics therein; meanwhile, under normal temperature storage conditions, the oil-based composition can effectively reduce the reduction of probiotic activity. In addition, the oil-based composition can also improve mouthfeel.

Description

Technical Field

[0001] The invention relates to the technical field of food and health food, and in particular to an oil-based composition and application thereof, a probiotic drop and a preparation method thereof. Background Art

[0002] Probiotics are active microorganisms that colonize the human body and change the host's intestinal microecology, thus benefiting intestinal health. The main functions of probiotics are to promote the digestion and absorption of nutrients, improve the body's immunity, maintain the balance of intestinal flora structure, inhibit intestinal inflammation and protect the intestinal mucosal barrier. However, the premise for probiotics to work is to maintain their activity before reaching the human intestine.

[0003] At present, the main forms of probiotics on the market are powders and tablets. The equipment cost of powder probiotics is high, the process is complicated, and it needs to be brewed with water when taken. Water with too high a temperature will reduce the number of viable probiotics. In addition, soaking in water for a long time will also cause the probiotics to decay. The tablets need to be dried at high temperature during processing and are very susceptible to moisture, which will reduce the number of viable probiotics. In addition, due to the limitation of dosage form, tablets are only suitable for children over three years old and adults, which is not conducive to the intake of probiotics by infants and young children.

[0004] Oil is one of the six nutrients needed by the human body and an important energy source. In addition, oil can help digest and absorb fat-soluble substances, such as fat-soluble vitamins A, D, E, and K, which can only be well absorbed in the presence of oil, otherwise it will lead to absorption disorders.

[0005] More than 99% of the oil is fat, and fat contains different types of fatty acids, which can be divided into saturated fatty acids and unsaturated fatty acids according to their structure. Saturated fatty acids are fatty acids containing saturated bonds, which are mostly found in animal fats, but plant fats such as coconut oil, cottonseed oil and palm oil are also rich in saturated fatty acids. Studies have shown that saturated fatty acids are the main cause of increased blood cholesterol, triglycerides, and low-density lipoprotein cholesterol (LDL-C), which in turn causes arterial lumen stenosis, the formation of atherosclerosis, and thus increases the risk of coronary heart disease. Unsaturated fatty acids are a type of fatty acid that constitutes body fat and are essential fatty acids for the human body. Unsaturated fatty acids are divided into monounsaturated fatty acids and polyunsaturated fatty acids, and polyunsaturated fatty acids are divided into linoleic acid, linolenic acid, arachidonic acid, etc. The human body cannot synthesize linoleic acid and linolenic acid, and must be supplemented from the diet. Unsaturated fatty acids can regulate blood lipids, clear blood clots, enhance immunity, maintain the retina and improve vision, nourish the brain, and improve arthritis symptoms and relieve pain. However, due to the poor stability of polyunsaturated fatty acids, lipid peroxidation reactions are prone to occur.

[0006] With the development of formulation technology, probiotic drops have emerged. Probiotic drops are a kind of drop product formed by dispersing probiotics in oil solvents, which can solve the problem of difficult feeding of infants and young children to a certain extent. However, related probiotic drops have the problem of bacterial powder depositing at the bottom of the package, especially during the shelf life, the bacterial powder will settle to the bottom of the package and stick to the bottom of the bottle, making it difficult to remix when using.

[0007] The patent application with publication number CN105707897A discloses a probiotic lipid suspension drop and a preparation method thereof. In order to solve the problems of poor suspension stability and precipitation and agglomeration of the probiotic drops, the prepared probiotic lipid suspension drops contain 68.4-72.6 ml of vegetable oil, 28.9 ml-30.7 ml of medium-chain acid glycerides, 0.0-1.2 g of sucrose monoglyceride, 0-0.5 g of vitamin E (50% content) and probiotic powder per 100 mL of the probiotic lipid suspension drops; the vegetable oil is selected from one or more of soybean oil, sesame oil, peanut oil, olive oil, sunflower seed oil, coconut oil and palm oil. The probiotic lipid suspension drops prepared by this patent have poor flavor and taste, and the stability test time is short; in addition, the sunflower oil process mostly uses the leaching method to extract, and there are trace amounts of organic solvents remaining, and long-term use is not conducive to the healthy growth of infants and young children; at the same time, sunflower oil, olive oil, soybean oil and other oils containing more unsaturated fatty acids are themselves easily oxidized, which is not conducive to maintaining the activity of probiotics.

[0008] Therefore, preventing bacterial powder deposition in probiotic drops is an urgent problem to be solved. Summary of the invention

[0009] Based on this, it is necessary to provide an oil-based composition and its application, a probiotic drops and its preparation method, so as to improve the stability of the probiotic drops and improve the sedimentation problem of the probiotic powder.

[0010] The first aspect of the present invention provides an oil-based composition comprising medium-chain triglycerides, DHA algae oil and walnut oil; the mass ratio of the medium-chain triglycerides, the walnut oil and the DHA algae oil is (3-4):(3-4):(1-3), optionally (3-3.8):(3-3.8):(1-2.4), and more optionally 3:3:(1-2).

[0011] In some embodiments, the mass ratio of the medium chain triglycerides, the walnut oil and the DHA algae oil is 3:3:1.

[0012] The second aspect of the present invention provides a use of the oil-based composition of the first aspect of the present invention in the preparation of food, medicine or health care product.

[0013] The third aspect of the present invention provides a use of the oil-based composition of the first aspect of the present invention in the preparation of probiotic drops.

[0014] The fourth aspect of the present invention provides a probiotic drop, comprising:

[0015] An oil-based composition, comprising medium-chain triglycerides, DHA algae oil and walnut oil; the mass ratio of the medium-chain triglycerides, the walnut oil and the DHA algae oil is (3-4):(3-4):(1-3); and

[0016] probiotics dispersed in the oil-based composition;

[0017] Optionally, the mass ratio of the medium-chain triglycerides, the walnut oil and the DHA algae oil is (3-3.8):(3-3.8):(1-2.4); more optionally, it is 3:3:(1-2).

[0018] In some embodiments, the mass of the oil-based composition accounts for 90%-99% of the total mass of the oil-based composition and the probiotics; the mass of the probiotics accounts for 1%-10% of the total mass of the oil-based composition and the probiotics.

[0019] In some embodiments, the mass ratio of the medium chain triglycerides, the walnut oil and the DHA algae oil is 3:3:1.

[0020] In some embodiments, the mass ratio of the medium-chain triglycerides, the walnut oil and the DHA algae oil is 3:3:1; the mass of the probiotics accounts for 4.1% of the total mass of the oil-based composition and the probiotics, and the mass of the oil-based composition accounts for 95.9% of the total mass of the oil-based composition and the probiotics.

[0021] In some embodiments, the probiotic drops have at least one of the following characteristics:

[0022] (1) The probiotics include one or more of bifidobacteria, lactobacilli and streptococci.

[0023] (2) The viable count of the probiotics is 7.5-60 billion CFU / g.

[0024] The fifth aspect of the present invention provides a method for preparing the probiotic drops of the third aspect of the present invention, comprising the following steps:

[0025] Mixing the DHA algae oil, the walnut oil and a portion of the medium-chain triglycerides to prepare an oil-based composition;

[0026] The probiotics are mixed and dispersed with the remaining medium-chain triglycerides and then mixed with the oil-based composition to prepare the probiotic drops.

[0027] The above-mentioned oil-based composition and its application, probiotic drops and preparation method thereof, wherein the oil-based composition comprises medium-chain triglycerides, walnut oil and DHA algae oil in a mass ratio of (3-4): (3-4): (1-3); by using three kinds of oils in a mass ratio within the above range to form the oil-based composition, the oil-based composition has good oil stability, can reduce or even avoid the sedimentation of probiotics therein, and can increase the dispersion speed of probiotics therein; at the same time, under room temperature storage conditions, the oil-based composition can effectively reduce the reduction of probiotic activity. In addition, the oil-based composition can also improve the taste.

[0028] The probiotic drops include an oil-based composition and probiotics dispersed in the oil-based composition. The probiotics have good stability and are not prone to sedimentation, and the dispersion speed of the probiotics in the oil-based composition is improved. In addition, the activity of the probiotics does not decrease significantly under room temperature storage conditions, thereby extending the shelf life. DETAILED DESCRIPTION

[0029] For ease of understanding of the present invention, the present invention will be described more fully below with reference to relevant embodiments. Preferred embodiments of the present invention are provided below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0031] In the present invention, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0032] In the present invention, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0033] Only some numerical ranges are specifically disclosed herein. However, any lower limit can be combined with any upper limit to form an unspecified range; and any lower limit can be combined with other lower limits to form an unspecified range, and likewise any upper limit can be combined with any other upper limit to form an unspecified range. In addition, each separately disclosed point or single value can itself be combined as a lower limit or upper limit with any other point or single value or with other lower limits or upper limits to form an unspecified range.

[0034] The temperature parameters in the present invention, if not specifically limited, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the precision range controlled by the instrument.

[0035] In the description of the invention, “plurality” means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0036] If there is no special description, all embodiments and optional embodiments of the present invention can be combined with each other to form a new technical solution. If there is no special description, all technical features and optional technical features of the present invention can be combined with each other to form a new technical solution.

[0037] If not specifically stated, all steps of the present invention may be performed sequentially or randomly, but are preferably performed sequentially.

[0038] In the related art, probiotic drops are mostly made of oils containing more unsaturated fatty acids, and probiotic drops have the problem of bacterial powder depositing at the bottom of the package. Especially during the shelf life, the bacterial powder will settle to the bottom of the package and stick to the bottom of the bottle, making it difficult to remix during use, and the dispersion is poor. At the same time, since unsaturated oils have the characteristic of being easily oxidized, the shelf life of commercially available probiotic drops is short, mostly 12-18 months. In addition, there is also the problem of rapid decline of probiotic live bacteria in commercially available probiotic preparations.

[0039] Based on the above problems, the first aspect of the present invention provides an oil-based composition, which includes medium-chain triglycerides, DHA algae oil and walnut oil; the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is (3-4):(3-4):(1-3), which can be (3-3.8):(3-3.8):(1-2.4), and can be more preferably 3:3:(1-2).

[0040] Medium-chain triglycerides, also known as medium-chain fatty acid triglycerides, are triglycerides composed of saturated medium-chain fatty acids with a carbon chain length of 6-12. They are a rare type of fat with a very small molecular structure. After entering the body, they can bypass the complex metabolic process and directly enter the liver for metabolism, quickly generating energy without being converted into stored fat. In addition, medium-chain triglycerides have good tolerability and safety and can be consumed with confidence. Since medium-chain triglycerides are stable in nature, when medium-chain triglycerides are used as one of the components of an oil-based composition, the stability of the oil-based composition can be improved.

[0041] DHA algae oil is pure plant DHA, commonly known as brain gold, which is a major element for the growth and maintenance of nervous system cells and an important component of the brain and retina. Its content in the human cerebral cortex is as high as 20%, and its content in the retina of the eye is as high as 50%. It is crucial for the intelligence and vision development of fetuses and infants. When DHA algae oil is used as one of the components of the oil-based composition, the dispersibility of the oil-based composition can be improved.

[0042] Walnut oil is a vegetable oil squeezed from walnut kernels. Its main fat components are linoleic acid glyceride, linolenic acid and oleic acid glyceride, which are essential fatty acids for the human body. Among common vegetable oils, walnut oil has the highest content of omega-3 (α-linolenic acid), which is beneficial for supplementing the linolenic acid required for the development of infant brain and vision. In addition, walnut oil is also rich in vitamins A, B, E, selenium, squalene, flavonoids and polyphenols, which are beneficial for enhancing human immunity and beautifying the skin. Walnut oil has a light taste and is suitable for direct oral consumption. When walnut oil is used as one of the components of the oil-based composition, the nutrition of the oil-based composition can be enriched and it is suitable for infants and young children. At the same time, walnut oil has no odor, is antioxidant, and has strong stability, which can improve the stability of the oil-based composition.

[0043] The mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is (3-4): (3-4): (1-3); for example, it can be (3-3.9): (3-3.9): (1-2.8), (3-3.8): (3-3.8): (1-2.6), (3-3.7): (3-3.7): (1-2.4), (3-3.6): (3-3.6): (1-2.2), (3-3.5): (3-3.5): (1-2), (3-3.4): (3-3.4): (1-1.8), (3-3.3): (3-3.3): (1-1.6), (3-3.2): (3-3. 2):(1-1.4) or (3-3.2):(3-3.2):(1-1.2, etc., are not specifically limited. Optionally, the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is (3-3.8):(3-3.8):(1-2.4). Further optionally, the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:(1-2). When the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:(1-2), when the probiotic drops are prepared together with probiotics, the oil-based composition is more stable and more conducive to reducing the decrease in probiotic activity. At the same time, the oil-based composition has no special aroma and taste and has strong palatability.

[0044] Understandably, by adopting three kinds of oils and fats with a mass ratio within the above range to form an oil-based composition, the oil-based composition has good oil stability, can reduce or even avoid the sedimentation of probiotics therein, and can increase the dispersion speed of probiotics therein; at the same time, under normal temperature storage conditions, the oil-based composition can effectively reduce the reduction of probiotic activity. In addition, the oil-based composition can also improve the mouthfeel. The viscosity, compatibility and stability of medium-chain triglycerides, walnut oil and DHA algae oil after mixing are good, and the antioxidant property is strong, the temperature adaptability range is wide, and it is not easy to solidify, not easy to stratify, and not easy to acidify corruption. In some embodiments, the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:1.8.

[0045] In some embodiments, the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:1; when they are used together with probiotics to prepare probiotic drops, the suspension effect is optimal and the probiotics can be prevented from depositing.

[0046] The second aspect of the present invention provides a use of the oil-based composition of the first aspect of the present invention in the preparation of food, medicine or health care product.

[0047] The third aspect of the present invention also provides an application of the oil-based composition of the first aspect of the present invention in preparing probiotic drops. Probiotics are dispersed in the oil-based composition to prepare probiotic drops, which can effectively reduce or avoid the deposition of probiotics, and the dispersion speed of probiotics in the oil-based composition is increased; in addition, the decrease in the activity of probiotics can be effectively reduced under normal temperature storage conditions.

[0048] The fourth aspect of the present invention also provides a probiotic drop, comprising: an oil-based composition and probiotics dispersed therein; the oil-based composition comprises medium-chain triglycerides, DHA algae oil and walnut oil; the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is (3-4):(3-4):(1-3); it can be optionally (3-3.8):(3-3.8):(1-2.4), and more optionally 3:3:(1-2).

[0049] The mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is (3-4): (3-4): (1-3); for example, it can be (3-3.9): (3-3.9): (1-2.8), (3-3.8): (3-3.8): (1-2.6), (3-3.7): (3-3.7): (1-2.4), (3-3.6): (3-3.6): (1-2.2), (3-3.5): (3-3.5): (1-2), (3-3.4): (3-3. 4):(1-1.8), (3-3.3):(3-3.3):(1-1.6), (3-3.2):(3-3.2):(1-1.4) or (3-3.2):(3-3.2):(1-1.2, etc., are not specifically limited. Optionally, the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is (3-3.8):(3-3.8):(1-2.4). Further optionally, the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is 3:3:(1-2).

[0050] Since medium-chain triglycerides are stable in nature, when medium-chain triglycerides are used to prepare probiotic drops, the stability of the oil-based composition can be improved.

[0051] When DHA algae oil is used to prepare probiotic drops, the dispersibility of the oil-based composition can be improved.

[0052] When walnut oil is used to prepare probiotic drops, it can enrich the nutrition of the oil-based composition and is suitable for use by infants and young children; at the same time, walnut oil has no odor, is antioxidant, and has strong stability, and can improve the stability of the oil-based composition.

[0053] Understandably, the probiotics dispersed in the oil-based composition have good stability and are not prone to sedimentation, and the dispersion speed of the probiotics in the oil-based composition is improved. In addition, the activity of the probiotics does not decrease significantly under room temperature storage conditions, extending the shelf life.

[0054] As a possible implementation, the mass percentage of the oil-based composition to the total mass percentage of the oil-based composition and the probiotics is 90%-99%; the mass percentage of the probiotics to the total mass percentage of the oil-based composition and the probiotics is 1%-10%. In the total mass formed by the probiotics and the oil-based composition, when the mass percentages of the probiotics and the oil-based composition are respectively within the above ranges, the solubility of the probiotics can be improved.

[0055] In some possible embodiments, the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is (3-3.8):(3-3.8):(1-2.4).

[0056] In some embodiments, the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is 3:3:1.8.

[0057] In some embodiments, the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is 3: 3: 1. In the probiotic drops, when the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil is 3: 3: 1, the suspension effect of probiotics in the oil-based composition is better, further reducing or avoiding the deposition of probiotics.

[0058] In some possible embodiments, the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:1; the mass of the probiotics accounts for 4.1% of the total mass of the oil-based composition and the probiotics, and the mass of the oil-based composition accounts for 95.9% of the total mass of the oil-based composition and the probiotics; the probiotics prepared in this way have the best probiotic dispersion effect, are not prone to sedimentation, and have the best effect on maintaining the activity of the probiotics, and have the longest shelf life under room temperature storage conditions; in addition, the taste is better.

[0059] In some embodiments, the probiotics include one or more of Bifidobacterium, Lactobacillus, and Streptococcus.

[0060] In some embodiments, the viable count of the probiotics is 7.5-60 billion CFU / g; for example, it can be but is not limited to 7.5 billion CFU / g, 10 billion CFU / g, 20 billion CFU / g, 30 billion CFU / g, 40 billion CFU / g, 50 billion CFU / g, 60 billion CFU / g or a range between any two of the above values.

[0061] The fifth aspect of the present invention provides a method for preparing the probiotic drops of the fourth aspect, comprising the following steps: mixing DHA algae oil, walnut oil and part of medium-chain triglycerides to prepare an oil-based composition; mixing and dispersing the probiotics with the remaining medium-chain triglycerides and then mixing them with the oil-based composition to prepare the probiotic drops.

[0062] The preparation method has simple process and can reduce production cost.

[0063] In some embodiments, the portion of medium chain triglycerides accounts for 70%-80% of the total amount of medium chain triglycerides; for example, it can be but not limited to 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80% or a range between any two of the above values. Using a small amount of medium chain triglycerides to dissolve probiotics is beneficial to improving the dispersion effect of probiotics in the oil-based composition.

[0064] The technical solution of the present invention is described in detail below in conjunction with specific embodiments.

[0065] 1. Preparation of probiotic drops

[0066] Example 1

[0067] Weigh the prescribed amount of medium chain triglycerides (hereinafter referred to as MCT), DHA algae oil and walnut oil respectively, mix 80% of the prescribed amount of MCT, the prescribed amount of DHA algae oil and the prescribed amount of walnut oil, mix them evenly by stirring, filter with a filter membrane, and place at room temperature;

[0068] Weigh the three-strain probiotic mixed powder in the prescribed amount, dissolve and mix 20% of the prescribed amount of MCT, and mix with the above mixture to prepare probiotic drops; wherein the components and percentages of the three-strain probiotic mixed powder are: Bifidobacterium lactis HN019 50%, Bifidobacterium lactis Bi-07 35% and Lactobacillus fermentum CECT5716 15%;

[0069] Adjust the equipment, control the net weight at an average of 10.0 mL per bottle, and the filling volume difference of each bottle is ±6.5%. Bottling is carried out, and the filling volume difference and appearance of the probiotic drops are checked every 30 minutes.

[0070] Embodiment 2-10

[0071] The preparation methods of the probiotic drops in Examples 2-10 are basically similar to the preparation method of the probiotic drops in Example 1, and the main differences are: at least one of the components of the oil-based composition and the amounts of each component, the usage ratio of each component, the mass proportion of the oil-based mixture in the probiotic drops, and the mass proportion of the probiotics in the probiotic drops is different. See Table 1 for details.

[0072] Comparative Example 1

[0073] 1. Prescription composition (reference CN201910330367.2)

[0074] 200 parts of walnut oil, 0.02 parts of isomaltooligosaccharide, 0.1 parts of medium-chain triglycerides, 10 parts of probiotic powder

[0075] 2. Preparation process

[0076] Weigh the above components according to the prescription amount, put walnut oil and isomaltooligosaccharide in a liquid preparation tank, cool the liquid preparation tank to below 10°C with low-temperature cooling water, and record the liquid in the liquid preparation tank as A1;

[0077] The weighed mixed powder of three probiotic strains and medium-chain triglycerides were added to A1 at a speed of 40 g / s, and deoxygenated and stirred at a negative pressure of 0.01 MPa, and the deoxygenated stirring speed was 200 rpm to obtain a full-oil anaerobic embedded suspension flocculent light yellow liquid; wherein the components and percentages of the mixed powder of three probiotic strains are: Bifidobacterium lactis HN019 50%, Bifidobacterium lactis Bi-07 35% and Lactobacillus fermentum CECT5716 15%;

[0078] Adjust the equipment, control the net weight at an average of 10.0 mL per bottle, and the filling volume difference of each bottle is ±6.5%. Bottling is carried out, and the filling volume difference and appearance of the probiotic drops are checked every 30 minutes.

[0079] Comparative Example 2 (reference CN202110212157.0)

[0080] 1. Prescription composition

[0081] 300 parts of olive oil, 628 parts of medium-chain triglycerides, 12 parts of probiotic powder, 0.4 parts of tea polyphenols

[0082] 2. Preparation process

[0083] Weigh olive oil and medium-chain triglycerides according to the prescription amount, mix them evenly, filter them through a precision filter, fill them into bottles, and sterilize them in an ultraviolet sterilizer;

[0084] Medium chain triglycerides and tea polyphenols are premixed first, and then mixed with olive oil and probiotics;

[0085] Adjust the equipment, control the net weight at an average of 10.0 mL per bottle, and the filling volume difference of each bottle is ±6.5%. Bottling is carried out, and the filling volume difference and appearance of the probiotic drops are checked every 30 minutes.

[0086] Comparative Example 3 (reference CN202110394014.0)

[0087] 1. Prescription composition

[0088] 1 part mono- and di-glycerol fatty acid esters, 89 parts medium-chain triglycerides, 10 parts probiotic powder

[0089] 2. Preparation process

[0090] Weigh mono- and di-glycerol fatty acid esters and medium-chain triglycerides according to the prescription amount and mix them evenly, then heat to 60°C until the mono- and di-glycerol fatty acid esters are completely melted. Then cool to 45°C, homogenize the mixture of mono- and di-glycerol fatty acid esters and medium-chain triglycerides at a homogenization pressure of 150Pa for 2 times to obtain a probiotic carrier;

[0091] Pour the probiotic carrier into a tank with a stirring paddle, put the probiotic powder into the tank and start stirring at a stirring speed of 80 rpm for 30 minutes to evenly mix the probiotic powder and the carrier; wherein the components and percentages of the three probiotic mixed powders are: Bifidobacterium lactis HN019 50%, Bifidobacterium lactis Bi-07 35% and Lactobacillus fermentum CECT571615%;

[0092] Fill into sterilized brown bottles, control the net weight at an average of 10.0 ml per bottle, and the filling volume difference of each bottle is ± 6.5%. Check the filling volume difference and appearance of the probiotic drops every 30 minutes.

[0093] Comparative Examples 4-6

[0094] The preparation methods of the probiotic drops in Comparative Examples 4-6 are basically similar to the preparation method of the probiotic drops in Example 1, and the main differences are: at least one of the components of the oil-based composition and the amounts of the components, the usage ratios of the components, the mass proportion of the oil-based mixture in the probiotic drops, and the mass proportion of the probiotics in the probiotic drops is different. See Table 1 for details.

[0095] The compositions of the probiotic drops in the above-mentioned embodiments and comparative examples are shown in Table 1.

[0096] Table 1

[0097]

[0098]

[0099] It should be noted that in Table 1, n1 is the mass ratio of medium chain triglycerides, walnut oil and DHA algae oil, w1 is the mass ratio of probiotics in the probiotic drops, and w2 is the mass ratio of the oil-based composition in the probiotic drops. The following tables are all the same.

[0100] The probiotic drops in Examples 1-10 and Comparative Examples 1-6 were tested for lactic acid bacteria content, appearance, total lactic acid bacteria count and peroxide value after accelerated growth (37° C., 75%) for 3 months. The results are shown in Table 2.

[0101] Table 2

[0102]

[0103] It should be noted that the theoretical probiotic results (*) are calculated based on the nominal values ​​of the supplier’s products. However, since probiotics are sensitive to environmental conditions such as temperature, humidity, and pH, in the actual supply process, the actual probiotic test values ​​of the products provided by the supplier are greater than the nominal values ​​to avoid failing to reach the nominal values ​​due to probiotic death. Therefore, the test values ​​of the final product after filling will also be greater than the nominal values.

[0104] From the results in Table 2, it can be seen that by adjusting the components of the oil-based composition, the mass ratio between the components, and the mass proportion of the oil-based composition and the probiotics in the probiotic drops, probiotic drops with good stability of the oil-based composition, significantly improved suspension and sedimentation of the probiotics, long-term preservation of the activity of the probiotics and suitable taste can be prepared.

[0105] Moreover, when the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:(1-2), the oil-based composition has better stability, better dispersibility of probiotics, and is not easy to deposit; and it is more conducive to maintaining the activity level of probiotics, and at the same time, the aroma and taste of the drops can be significantly improved, the drops have no special aroma and taste, and the palatability is improved. When the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:1, and the mass proportion of probiotics is 4.1%, and the mass proportion of the oil-based composition is 95.9%, the prepared probiotic drops have the best dispersion effect, the longest shelf life under room temperature storage conditions, and the best taste.

[0106] Examples 11-12

[0107] 1. The probiotic powder in Examples 11-12 is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716, and the prescription composition is shown in Table 3.

[0108] Table 3

[0109]

[0110] 2. The preparation method is the same as that in Example 1; it should be noted that, in the production process of the above-mentioned probiotic drops, the same lactic acid bacteria powder with different contents is replaced under the same production environment, the same mixing equipment, and the same filling machine.

[0111] The probiotic drops in Example 1 and Examples 11-12 were investigated for lactic acid bacteria content after filling, stability of lactic acid bacteria content after opening for 30 days, and stability of lactic acid bacteria content after accelerated use for 3 months. The results are shown in Table 4.

[0112] Table 4

[0113] Detection indicators Example 1 Embodiment 11 Example 12 Probiotic test results after canning, 100 million CFU / g 88 176 345 Total number of probiotics after opening (37℃) for 30 days, 100 million CFU / g 77 154 300 Accelerated (37℃, 75%) probiotic count after 3 months, 100 million CFU / g 71 143 278

[0114] From the results in Table 4, it can be seen that when the oil-based composition composed of medium-chain triglycerides, walnut oil and DHA algae oil is used to prepare probiotic drops together with different added amounts of probiotic powder, the prepared probiotic drops all have good probiotic activity stability, that is, they all have a good probiotic activity maintenance effect.

[0115] Examples 13-15

[0116] 1. Prescription composition

[0117] The probiotic powder used in Examples 13-15 is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716, and the formula is shown in Table 5.

[0118] Table 5

[0119]

[0120] 2. The preparation process is the same as that in Example 1.

[0121] Comparative Example 7

[0122] 1. Prescription composition

[0123] The probiotic powder used in Comparative Example 7 is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716, and the prescription is shown in Table 6.

[0124] Table 6

[0125]

[0126] The probiotic drops in Examples 13-15 and Comparative Example 7 were tested for appearance and taste, and the specific method was as follows:

[0127] After the probiotic drops were left to stand for 1 month, based on the experimental design method in statistics and considering the representativeness of consumers on the product side, 10 males and 10 females, aged from 25 to 40 years old, were recruited. Each person scored each oil sample. Appearance scoring criteria: 0-3 points: the probiotic powder clumps and sinks to the bottom, and cannot be dispersed by shaking vigorously; 4-7 points: the probiotic powder sinks to the bottom, and can be mixed by shaking it slightly; 8-10 points: the probiotic powder is evenly dispersed / the probiotic powder sinks slightly to the bottom, and it is restored to uniformity by shaking it slightly. Taste scoring criteria: 0-3 points, with the inherent smell and taste of a certain oil, with peculiar smell; 4-7 points, with the inherent smell and taste of a certain oil, without peculiar smell; 8-10 points, no smell, good taste and good. The test results are shown in Table 7.

[0128] Table 7

[0129]

[0130] From the comparison of the results of Examples 13-15 and Comparative Example 7, it can be seen that the addition of DNA algae oil can effectively improve the dispersibility of the probiotic powder and prevent the probiotic powder from sinking to the bottom and sticking. From the comparison of the results of Examples 13-15, it can be seen that when the mass ratios of medium-chain triglycerides, walnut oil and DHA algae oil are different, the taste difference of the drops is not obvious; when the mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil is 3:3:1, the suspension effect of probiotics is the best.

[0131] Example 16

[0132] 1. Prescription composition

[0133] 300 parts of medium chain triglycerides, 300 parts of walnut oil, 100 parts of DHA algae oil, and 30 parts of bacterial powder; wherein the bacterial powder is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07, and 15% of Lactobacillus fermentum CECT5716.

[0134] 2. The preparation process is the same as that in Example 1.

[0135] Comparative Example 8

[0136] 1. Prescription composition

[0137] 300 parts of medium chain triglycerides, 300 parts of sunflower oil, 100 parts of DHA algae oil, and 30 parts of bacterial powder; wherein the bacterial powder is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716.

[0138] 2. The preparation process is the same as that in Example 1.

[0139] Comparative Example 9

[0140] 1. Prescription composition

[0141] 300 parts of walnut oil, 300 parts of sunflower seed oil, 100 parts of DHA algae oil and 30 parts of bacterial powder; wherein the bacterial powder is 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716.

[0142] 2. The preparation process is the same as that in Example 1.

[0143] Since the temperature of the transport container can reach above 45°C during the transportation of probiotic drops, a 3-month accelerated inspection at 45°C and a long-term 24-month follow-up inspection under normal temperature shelf conditions were set up.

[0144] The probiotic drops prepared in Example 16 and Comparative Examples 8-9 were placed in an incubator at 45°C and RH75% and in a normal temperature container, and samples were taken for inspection every month. Since the shelf life of most competing products on the market is 12-18 months, the results of 18-month and 24-month samples at room temperature were compared and analyzed. On the basis of ensuring that there is no obvious change in the appearance of the product, a combination of [peroxide value] less than [10μg / kg] and [acid value] less than [1.5mg KOH / g] is preferred. The inspection results are shown in Table 8.

[0145] Table 8

[0146]

[0147] From the comparison of the results of Example 16 and Comparative Examples 8-9 in the table, it can be seen that the peroxide value of the oil-based composition without medium-chain triglycerides exceeds the quality requirement of less than 10 μg / kg for the peroxide value of oils and fats in the national standard; while the combination of medium-chain triglycerides and sunflower oil just meets the national standard at the 18th month, but does not meet the requirements of the national standard at the 24th month. The combination of medium-chain triglycerides, walnut oil and algae oil DHA can still guarantee the daily dosage on the product label at the 24th month; therefore, the combination of medium-chain triglycerides, walnut oil and algae oil DHA as an oil-based composition is used to prepare probiotic drops, which prolongs the shelf life of the drops.

[0148] Example 17 and Comparative Examples 10-18

[0149] 1. Prescription composition

[0150] The prescription compositions of Example 17 and Comparative Examples 10-18 are shown in Table 9. It should be noted that in Example 17 and Comparative Examples 10-18, 20 billion CFU / g of probiotic powder (*) was added, and the probiotic powder used was 50% of Bifidobacterium lactis HN019, 35% of Bifidobacterium lactis Bi-07 and 15% of Lactobacillus fermentum CECT5716.

[0151] 2. The preparation process is the same as that in Example 1.

[0152] Table 9

[0153] serial number Oil-based compositions Probiotics Embodiment 17 MCT, walnut oil and algae oil DHA algae oil in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 10 MCT, sunflower oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 11 MCT, coconut oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 12 MCT, tea oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 13 Walnut oil, sunflower seed oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 14 Walnut oil, coconut oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 15 Walnut oil, tea oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 16 Sunflower oil, coconut oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 17 Sunflower oil, tea oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder Comparative Example 18 Coconut oil, tea oil and algae oil DHA in a mass ratio of 3:3:1 20 billion CFU / g probiotic powder

[0154] The probiotic drops in Example 17 and Comparative Examples 10-18 were subjected to weight sampling test and total viable bacteria test of the drop samples. It should be noted that 20 billion CFU / g of probiotic powder (*) is the nominal value of the supplier's product, but since probiotics are sensitive to environmental conditions such as temperature, humidity, and pH, in the actual supply process, the actual test values ​​of the probiotics in the products provided by the supplier are all greater than the nominal values ​​to avoid failing to reach the nominal values ​​due to the death of probiotics, so the test values ​​of the final product after filling will also be greater than the nominal values.

[0155] Weight sampling test: Take 6 drops of liquid as a group and test the repeatability of the weight of the dripped liquid. It is preferred to select a combination of 6 drops with a total weight of 260 mg and a drip error of ±30 mg. The test results are shown in Tables 10-1 to 10-4; the relative standard deviation results are shown in Table 11.

[0156] Detection of total number of viable bacteria in dripping samples: Screen the embodiments or comparative examples that pass the weight test, collect the samples of dripping samples, weigh them, and detect the total number of probiotics in proportion according to the detection method of GB4789.35. Count the total number of probiotics dripped from each group of drops to reflect the dispersibility of the oil-based composition for probiotics. The combination with a detection error floating range of ±10% is preferred. The results are shown in Tables 12-1 to 12-3; the mean and error floating range results of each group are shown in Table 13.

[0157] Table 10-1

[0158]

[0159] Table 10-2

[0160]

[0161] Table 10-3

[0162]

[0163] Table 10-4

[0164]

[0165] Table 11

[0166]

[0167] It should be noted that in Comparative Examples 11, 16 and 18, turbidity and caking appeared at the bottom of the probiotic drops at the end of the test. The reason may be that the drop weight test was carried out at room temperature in winter, which was low (14°C). The oil-based composition containing coconut oil partially solidified, resulting in caking at the bottom.

[0168] Table 12-1

[0169]

[0170] Table 12-2

[0171]

[0172] Table 12-3

[0173]

[0174] Table 13

[0175]

[0176] As can be seen from the results in the above tables, coconut oil has a high freezing point and is prone to solidification during daily use, and repeated freeze-thaw processes easily destroy the stability of probiotic activity, so adding coconut oil as an oil-based composition is not conducive to preparing drops with stable probiotic activity. Tea oil contains a large amount of tea polyphenols, which have a bactericidal effect. In probiotic drops, it will have a greater impact on the activity of probiotics. After the drops are placed for one month, the total number of lactic acid bacteria has a large loss, and the quality of the probiotics in the later period of the shelf life of the drops cannot be guaranteed.

[0177] 2. Performance test of supplementary edible oil

[0178] 1. Fatty acid composition

[0179] Commonly used oils and fats: MCT oil (medium chain triglycerides), coconut oil, olive oil, palm oil, sesame oil, corn oil, cottonseed oil, sunflower oil, peanut oil, soybean oil, tea oil, rapeseed oil, walnut oil, lard. The specific results of the fatty acid composition of oils and fats are shown in Table 14.

[0180] Table 14

[0181] grease Saturated fatty acids Monounsaturated fatty acids Linoleic acid α-linolenic acid Medium Chain Triglycerides 51.5 36.7 8.9 2.9 Coconut Oil 92 0 6 2 olive oil 15.5 71.2 12.3 1 Palm Oil 43.4 44.4 12.1 0 Sesame oil 14.1 39.4 45.6 0.8 Corn Oil 14.5 27.7 56.4 0.6 Cottonseed oil 24.3 27 44.3 0.4 Sunflower Oil 14 19.3 63.2 4.5 Peanut Oil 18.5 40.8 37.9 0.4 Soybean Oil 15.9 24.7 51.7 6.7 tea oil 10 78.8 10 1.1 Rapeseed Oil 13.2 58.8 16.3 8.4 Walnut Oil 8 22 56 14 lard 43.2 47.9 8.9 0

[0182] If too much saturated fatty acids are consumed in the diet, the cholesterol content in the blood will increase, which is significantly correlated with the incidence and mortality of hyperlipidemia and coronary heart disease; in addition, the saturated fatty acids in MCT oil and coconut oil are mainly composed of medium-chain fatty acid triglycerides, which are easily absorbed in the body, metabolized quickly and are not converted into fat storage, and can remain stable at both high and low temperatures; therefore, MCT oil (medium-chain triglycerides), coconut oil, olive oil, sesame oil, sunflower oil, peanut oil, soybean oil, tea oil, rapeseed oil, and walnut oil can be selected as types of oils for infants and young children, and can be used as backup ingredients for preparing probiotic drops.

[0183] 2. Taste test

[0184] The taste scores of MCT (medium chain triglycerides), walnut oil, sunflower oil, coconut oil, olive oil, peanut oil, sesame oil, corn oil, soybean oil, tea oil and rapeseed oil were investigated. The specific method is as follows:

[0185] The oil monomers were weighed according to the test amount, and the oil monomers were scored and tested for the "smell and taste" index. The taste test plan was designed based on the Design of Experiment (DOE) method in statistics. Considering the representativeness of consumers on the product side, 10 males and 10 females, aged from 25 to 40 years old, were parents with children. Each person scored each oil monomer sample. 0-3 points, with a certain oil inherent smell and taste, with peculiar smell; 4-7 points, with a certain oil inherent smell and taste, without peculiar smell; 8-10 points, no smell, good taste and good. The test results are shown in Table 15 below.

[0186] Table 15

[0187]

[0188] From the results in Table 15, it can be seen that MCT oil, walnut oil and sunflower seed oil have no specific smell and taste, are highly palatable and have the highest acceptance; coconut oil, olive oil, corn oil, rapeseed oil and tea oil are highly palatable, but have a heavier oil taste.

[0189] 3. Accelerated investigation experiment of oil and fat mixture

[0190] The experimental design DOE screening method investigated and analyzed the appearance, peroxide value and acid value stability of the composition of medium-chain triglycerides, walnut oil, sunflower oil, coconut oil, olive oil, corn oil, rapeseed oil and tea oil mixed in pairs in a ratio of 1:1 in the accelerated investigation; the specific method is as follows: weigh the above 8 oil bases according to the formula amount and mix them in equal parts; divide the above mixture into sterilized and dried glass bottles, place them at 37°C and 75% humidity for 3 months, and take samples every month for testing. The above mixture was sampled every month for appearance monitoring, and the results are shown in Table 16. The above mixture was sampled every month, and the peroxide value of the oil was detected according to the first method of GB5009.227-2016. The combination with a [peroxide value] less than [10μg / kg] was preferred, and the results are shown in Table 17. The above mixture was sampled every month and the acid value of the oil was measured according to GB5009.229-1016. The combination with an acid value less than 1.5 mg KOH / g was preferred. The results are shown in Table 18.

[0191] Table 16

[0192]

[0193]

[0194] Table 17

[0195]

[0196] Table 18

[0197]

[0198] From the results in Tables 16-18, it can be seen that the peroxide value test results of olive oil, corn oil and rapeseed oil do not meet the quality and safety requirements specified in the national standards and are not suitable as components of the oil-based composition of probiotic drops.

[0199] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0200] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A probiotic drop, characterized in that: include: An oil-based composition, the oil-based composition consisting of medium-chain triglycerides, walnut oil and DHA algae oil in a mass ratio of 3:3:(1-2); and Probiotics are dispersed in the oil-based composition; the probiotics are selected from one or more of bifidobacteria, lactobacilli and streptococci; the mass of the oil-based composition accounts for 90%-99% of the total mass of the oil-based composition and the probiotics; the mass of the probiotics accounts for 1%-10% of the total mass of the oil-based composition and the probiotics.

2. The probiotic drops according to claim 1, characterized in that The mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil in the oil-based composition is 3:3:

1.

3. The probiotic drops according to claim 1, characterized in that The mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil in the oil-based composition is 3:3:1.

5.

4. The probiotic drops according to claim 1, characterized in that The mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil in the oil-based composition is 3:3:1.

8.

5. The probiotic drops according to claim 1, characterized in that The mass ratio of medium chain triglycerides, walnut oil and DHA algae oil in the oil-based composition is 3:3:

2.

6. The probiotic drops according to claim 1, characterized in that The mass ratio of medium-chain triglycerides, walnut oil and DHA algae oil in the oil-based composition is 3:3:1; the mass of the probiotics accounts for 4.1% of the total mass of the oil-based composition and the probiotics, and the mass of the oil-based composition accounts for 95.9% of the total mass of the oil-based composition and the probiotics.

7. The probiotic drops according to any one of claims 1 to 6, characterized in that The viable bacterial count of the probiotics is 7.5-60 billion CFU / g.

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