A composition for resisting photoaging and skin aging

Abstract

The invention discloses a composition for resisting photoaging and skin aging, and belongs to the field of skin care products. The composition comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other auxiliary materials. The red ganoderma extract, sandalwood extract, madecassoside and peony root bark extract used in the invention have unexpected synergistic antioxidant capacity, anti-wrinkle and other effects, can resist light pollution from the inside of the skin, reduce free radical formation, inhibit inflammation and erythema formation, and resist skin photoaging and skin aging.

Description

Technical Field

[0001] The invention relates to the field of skin care products, and in particular to a composition for resisting photoaging and skin aging. Background Art

[0002] The aging process of the skin includes intrinsic aging and exogenous aging. Endogenous aging is caused by physiological factors such as cell aging, which is mainly triggered by oxidative stress of skin cells. Exogenous aging is the result of environmental factors. Long-term exposure to ultraviolet radiation is the main factor of exogenous skin aging. Therefore, exogenous aging is mainly a photoaging process. The significant characteristics of skin photoaging are mainly reflected in three aspects: sagging skin and wrinkles; impaired barrier function and dry and rough surface; pigmentation, spots, sun spots, etc.

[0003] Photoaging is an aging change in the skin due to long-term cumulative exposure to sunlight. Ultraviolet rays pass through the epidermis to the papillary dermis, causing sunburn cells to form in the epidermis. Ultraviolet rays can cause direct DNA damage by promoting the formation of cyclic pyrimidine dimers; they can also promote the formation of other photoproducts such as reactive oxygen species, matrix metalloproteinases, and inflammatory factors such as IL-6 that are associated with skin cancer and skin aging. Once the skin is exposed to ultraviolet radiation, multiple signaling pathways in skin cells that are responsible for promoting photoaging and skin cancer by regulating cell growth, proliferation, and survival are activated, leading to skin changes. In dermal cells such as fibroblasts, ultraviolet rays inhibit the synthesis of type I procollagen. At the same time, overexpression of matrix metalloproteinases leads to a decrease in collagen and elastin content, and degradation of structural proteins in the dermis that maintain skin elasticity.

[0004] At present, there are mainly the following methods for anti-photoaging:

[0005] Method 1: Based on the principle of physical sun protection, titanium dioxide, zinc oxide and other raw materials are used to reflect sunlight to achieve the effect of sun protection. The quality of this type of product depends on the processing quality of the reflective particles. Generally, ultraviolet rays can reach the skin through the gaps between the particles. However, this method has the following disadvantages: the product contains a large number of reflective particles, which can easily clog pores; it is generally thick and not suitable for sensitive skin and acne skin; makeup removal is required after use, which is inconvenient; it is generally only used when going out, and can only resist partial ultraviolet damage, and can only passively resist light pollution and photoaging.

[0006] Method 2: Use a combination of physical and chemical UV absorbers to resist light pollution by reflecting and absorbing harmful UV rays. Since chemical sunscreen molecules are absorbed by the skin, the process of absorbing UV rays occurs inside the skin and is metabolized and removed by the human body. However, this method has the following disadvantages: chemical sunscreens are irritating to the skin, the product has a high oil content, which can easily lead to acne formation, is not suitable for sensitive skin and acne-prone skin, requires makeup removal after use, which is inconvenient, and is generally only used when going out. It has strong limitations and can only passively resist light pollution and photoaging.

[0007] Method 3: Use blue light absorbers such as calendula extract, Sophora japonica extract, and hesperidin to prevent blue light in sunlight and LED blue light from reaching the skin and causing damage. However, this method has the following disadvantages: it is only suitable for the protection of LED blue light, and cannot resist the light damage caused by sunlight; it can only passively resist the damage caused by LED blue light, and cannot actively resist the light aging caused by LED blue light.

[0008] Therefore, it is still necessary to develop a composition that can actively resist photoaging by resisting light pollution, reducing free radical formation, repairing damaged cell DNA, inhibiting inflammation and erythema formation, etc. from within the skin. Summary of the invention

[0009] In view of the above problems, the present invention aims to provide a composition and a preparation method and application thereof.

[0010] In a first aspect, the present invention provides a composition.

[0011] A composition comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other auxiliary materials. The red ganoderma extract, sandalwood extract, madecassoside and peony root bark extract used in the present invention have unexpected synergistic antioxidant capacity, anti-wrinkle and other effects, can resist light pollution from the inside of the skin, reduce free radical formation, inhibit inflammation and erythema formation, and resist skin photoaging and skin aging.

[0012] In some embodiments, the other excipients include at least one of a solubilizer, a humectant, and a preservative.

[0013] In some embodiments, the solvent is water.

[0014] In some embodiments, the solubilizing agent is SOLUBILISANT LRI.

[0015] In some embodiments, the humectant includes at least one of glycerol, allantoin, trehalose, and betaine. In some preferred embodiments, the humectant is glycerol.

[0016] In some embodiments, the preservative includes at least one of p-hydroxyacetophenone, 1,3-butanediol, 1,2-pentanediol, and 1,2-hexanediol. In some preferred embodiments, the preservative is p-hydroxyacetophenone and 1,3-butanediol.

[0017] In some embodiments, the composition comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other excipients; the other excipients comprise a solubilizer, a humectant and a preservative; the solvent is water; the solubilizer is SOLUBILISANT LRI; the humectant comprises at least one of glycerin, allantoin, trehalose and betaine; the preservative comprises at least one of p-hydroxyacetophenone, 1,3-butylene glycol, 1,2-pentanediol and 1,2-hexanediol;

[0018] The peony root bark extract contains 0.2wt%-1.0wt% of peony root bark extract, 40wt%-60wt% of 1,3-butanediol, and the balance is water;

[0019] The red ganoderma extract is ganoderma spore oil;

[0020] The sandalwood extract contains 0.4wt%-1.0wt% of sandalwood extract, and the remainder is octyl dodecanol.

[0021] In some embodiments, the composition comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other excipients; the other excipients comprise a solubilizer, a humectant and a preservative; the solvent is water; the solubilizer is SOLUBILISANT LRI; the humectant comprises at least one of glycerin, allantoin, trehalose and betaine; the preservative comprises at least one of p-hydroxyacetophenone, 1,3-butylene glycol, 1,2-pentanediol and 1,2-hexanediol;

[0022] The peony root bark extract contains 0.2wt%-1.0wt% of peony root bark extract, 40wt%-60wt% of 1,3-butanediol, and the balance is water;

[0023] The red ganoderma extract is ganoderma spore oil;

[0024] The sandalwood extract contains 0.4wt%-1.0wt% of sandalwood extract, and the remainder is octyl dodecanol;

[0025] Calculated based on the total mass of the composition, the content of madecassoside is 0.10000wt%-0.30000wt%; the content of the peony root bark extract is 0.01000wt%-0.10000wt%; the content of the red ganoderma extract is 0.00010wt%-0.00100wt%; the content of the sandalwood extract is 0.00001wt%-0.00010wt%; the content of the SOLUBILISANT LRI is 0.10000wt%-1.00000wt%; the content of the humectant is 5.00000wt%-15.00000wt%; the content of the preservative is 1.20000wt%-5.50000wt%; and the balance is water.

[0026] In some embodiments, the peony root bark extract contains 0.2wt%-1.0wt% of peony root bark extract. In some embodiments, the peony root bark extract contains 0.2wt%-1.0wt% of peony root bark extract, 40wt%-60wt% of 1,3-butanediol, and the balance is water. In some embodiments, the peony root bark extract contains 0.2wt%-0.6wt% of peony root bark extract, 45wt%-55wt% of 1,3-butanediol, and the balance is water. In some embodiments, the peony root bark extract contains 0.4wt%-0.6wt% of peony root bark extract, 45wt%-55wt% of 1,3-butanediol, and the balance is water. In some embodiments, the peony root bark extract contains 0.4wt% of peony root bark extract, 50wt% of 1,3-butanediol and 49.6wt% of water. In some embodiments, the peony root bark extract is a product with the trade name of Gerbex Suffruticosa.

[0027] In some embodiments, the Ganoderma lucidum extract is Ganoderma lucidum spore oil.

[0028] In some embodiments, the sandalwood extract contains 0.4wt%-1.0wt% of sandalwood extract. In some embodiments, the sandalwood extract contains 0.4wt%-1.0wt% of sandalwood extract, and the balance is octyldodecanol. In some embodiments, the sandalwood extract contains 0.4wt%-0.8wt% of sandalwood extract, and the balance is octyldodecanol. In some embodiments, the sandalwood extract contains 0.4wt%-0.7wt% of sandalwood extract, and the balance is octyldodecanol. In some embodiments, the sandalwood extract contains 0.4wt%-0.6wt% of sandalwood extract, and the balance is octyldodecanol. In some embodiments, the sandalwood extract contains 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt% or 1.0wt% of sandalwood extract, and the balance is octyldodecanol. In some embodiments, the sandalwood extract contains 0.5 wt% of sandalwood extract, and the remainder is octyl dodecanol. In some embodiments, the sandalwood extract is a product with a trade name of santalwood biofunctional.

[0029] In some embodiments, the content of madecassoside is 0.10000wt%-0.30000wt% based on the total mass of the composition. In some embodiments, the content of madecassoside is 0.10000wt%, 0.15000wt%, 0.20000wt%, 0.25000wt% or 0.30000wt% based on the total mass of the composition. The active ingredient madecassoside of the present invention has such a content range that it is beneficial to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and is beneficial to obtain better anti-photoaging and anti-skin aging effects, and has unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0030] In some embodiments, the content of the peony root bark extract is 0.01000wt%-0.10000wt% based on the total mass of the composition. In some embodiments, the content of the peony root bark extract is 0.01000wt%, 0.02000wt%, 0.03000wt%, 0.04000wt%, 0.05000wt%, 0.06000wt%, 0.07000wt%, 0.08000wt%, 0.09000wt% or 0.10000wt% based on the total mass of the composition. The peony root bark extract of the active component of the present invention adopts this content range to better achieve excellent antioxidant ability, anti-wrinkle and other effects, and is conducive to obtaining better anti-photoaging and anti-skin aging effects, and has unexpected synergistic technical effects in terms of antioxidant ability, anti-wrinkle, anti-photoaging and anti-skin aging.

[0031] In some embodiments, the content of the red ganoderma extract is 0.00010wt%-0.00100wt% based on the total mass of the composition. In some embodiments, the content of the red ganoderma extract is 0.00010wt%, 0.00010wt%, 0.00020wt%, 0.00030wt%, 0.00040wt%, 0.00050wt%, 0.00060wt%, 0.00070wt%, 0.00080wt%, 0.00090wt% or 0.00100wt% based on the total mass of the composition. The red ganoderma extract of the active component of the present invention adopts this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and is conducive to obtaining better anti-photoaging and anti-skin aging effects, and has unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0032] In some embodiments, the content of the sandalwood extract is 0.00001wt%-0.00010wt% based on the total mass of the composition. In some embodiments, the content of the sandalwood extract is 0.00001wt%, 0.00002wt%, 0.00003wt%, 0.00004wt%, 0.00005wt%, 0.00006wt%, 0.00007wt%, 0.00008wt%, 0.00009wt% or 0.00010wt% based on the total mass of the composition. The sandalwood extract of the active component of the present invention adopts this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and is conducive to obtaining better anti-photoaging and anti-skin aging effects, and has unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0033] In some embodiments, the content of the SOLUBILISANT LRI is 0.10000wt%-1.00000wt% based on the total mass of the composition. In some embodiments, the content of the SOLUBILISANT LRI is 0.20000wt%-0.80000wt% based on the total mass of the composition. In some embodiments, the content of the SOLUBILISANT LRI is 0.30000wt%-0.70000wt% based on the total mass of the composition. In some embodiments, the content of the SOLUBILISANT LRI is 0.40000wt%-0.60000wt% based on the total mass of the composition. In some embodiments, the content of the SOLUBILISANT LRI is 0.50000wt% based on the total mass of the composition. In some embodiments, based on the total mass of the composition, the content of the SOLUBILISANT LRI is 0.10000wt%, 0.20000wt%, 0.30000wt%, 0.40000wt%, 0.50000wt%, 0.60000wt%, 0.70000wt%, 0.80000wt%, 0.90000wt% or 1.00000wt%.

[0034] In some embodiments, the content of the humectant is 5.00000wt%-15.00000wt% based on the total mass of the composition. In some embodiments, the content of the humectant is 8.00000wt%-12.00000wt% based on the total mass of the composition. In some embodiments, the content of the humectant is 9.00000wt%-11.00000wt% based on the total mass of the composition. In some embodiments, the content of the humectant is 10.00000wt% based on the total mass of the composition. In some embodiments, the content of the moisturizer is 5.00000wt%, 6.00000wt%, 7.00000wt%, 8.00000wt%, 9.00000wt%, 10.00000wt%, 11.00000wt%, 12.00000wt%, 13.00000wt%, 14.00000wt% or 15.00000wt%, calculated based on the total mass of the composition.

[0035] In some embodiments, the content of p-hydroxyacetophenone is 0.20000wt%-0.50000wt% based on the total mass of the composition. In some embodiments, the content of p-hydroxyacetophenone is 0.20000wt%-0.40000wt% based on the total mass of the composition. In some embodiments, the content of p-hydroxyacetophenone is 0.30000wt% based on the total mass of the composition. In some embodiments, the content of p-hydroxyacetophenone is 0.20000wt%, 0.30000wt%, 0.40000wt% or 0.50000wt% based on the total mass of the composition.

[0036] In some embodiments, the content of 1,3-butanediol in the preservative in the composition is 1.00000wt%-5.00000wt% based on the total mass of the composition. In some embodiments, the content of 1,3-butanediol in the preservative in the composition is 1.00000wt%-3.00000wt% based on the total mass of the composition. In some embodiments, the content of 1,3-butanediol in the preservative in the composition is 1.50000wt%-2.50000wt% based on the total mass of the composition. In some embodiments, the content of 1,3-butanediol in the preservative in the composition is 2.00000wt% based on the total mass of the composition. In some embodiments, the content of 1,3-butanediol in the preservative in the composition is 1.00000wt%, 2.00000wt%, 3.00000wt%, 4.00000wt% or 5.00000wt% based on the total mass of the composition.

[0037] In some embodiments, the other excipients include a solubilizer, a humectant and a preservative; the humectant is glycerol, and the preservative is p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of madecassoside is 0.10000wt%-0.30000wt%; the content of the peony root bark extract is 0.01000wt%-0.10000wt%; the content of the red ganoderma extract is 0.00010wt%-0.00100wt%; the content of the sandalwood extract is 0.00001wt%-0.00010wt%; the SOLUBILISANT The content of LRI is 0.10000wt%-1.00000wt% or 0.20000wt%-0.80000wt% or 0.30000wt%-0.70000wt% or 0.40000wt%-0.60000wt% or 0.50000wt%; the content of the humectant is 5.00000wt%-15.00000wt% or 8.00000wt%-12.00000wt% or 9.00000wt%-11.00000wt% or The content of p-hydroxyacetophenone is 0.20000wt%-0.50000wt% or 0.20000wt%-0.40000wt% or 0.30000wt%; the content of 1,3-butanediol in the preservative in the composition is 1.00000wt%-5.00000wt% or 1.00000wt%-3.00000wt% or 1.50000wt%-2.50000wt% or 2.00000wt%; the balance is water. The active components of the present invention (madecassoside, peony root bark extract, red ganoderma extract, and sandalwood extract) are respectively used in this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and to obtain better anti-photoaging and anti-skin aging effects, and have unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0038] In some embodiments, the other excipients include a solubilizer, a humectant and a preservative; the humectant is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxymadecassoside is 0.10000wt%; the content of the peony root bark extract is 0.01000wt%; the content of the red ganoderma extract is 0.00010wt%; the content of the sandalwood extract is 0.00001wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the humectant is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; and the balance is water. The active components of the present invention (madecassoside, peony root bark extract, red ganoderma extract, and sandalwood extract) are respectively used in this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and to obtain better anti-photoaging and anti-skin aging effects, and have unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0039] In some embodiments, the other excipients include a solubilizer, a humectant and a preservative; the humectant is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxymadecassoside is 0.20000wt%; the content of the peony root bark extract is 0.05000wt%; the content of the red ganoderma extract is 0.00050wt%; the content of the sandalwood extract is 0.00005wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the humectant is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; and the balance is water. The active components of the present invention (madecassoside, peony root bark extract, red ganoderma extract, and sandalwood extract) are respectively used in this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and to obtain better anti-photoaging and anti-skin aging effects, and have unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0040] In some embodiments, the other excipients include a solubilizer, a humectant and a preservative; the humectant is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxymadecassoside is 0.30000wt%; the content of the peony root bark extract is 0.10000wt%; the content of the red ganoderma extract is 0.00100wt%; the content of the sandalwood extract is 0.00010wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the humectant is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; and the balance is water. The active components of the present invention (madecassoside, peony root bark extract, red ganoderma extract, and sandalwood extract) are respectively used in this content range to better achieve excellent antioxidant capacity, anti-wrinkle and other effects, and to obtain better anti-photoaging and anti-skin aging effects, and have unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0041] In some embodiments, the composition is in a dosage form for external use.

[0042] In some embodiments, the composition is in the form of an aqueous solution, a spray or an aerosol.

[0043] In some embodiments, the composition is in the form of an external aqueous solution, an external spray, or an external aerosol.

[0044] In a second aspect, the present invention provides a method for preparing the composition of the first aspect.

[0045] A method for preparing the composition of the first aspect, wherein the other excipients include a solubilizer, a moisturizer and a preservative; the preservative includes p-hydroxyacetophenone and 1,3-butanediol; the preparation method comprises:

[0046] (1) mixing the red ganoderma extract, the sandalwood extract, the solubilizer and water to obtain a mixed solution A;

[0047] (2) mixing p-hydroxyacetophenone and 1,3-butanediol and heating them until dissolved, cooling to room temperature to obtain a mixed solution B, and mixing the mixed solution B with the mixed solution A to obtain a mixed solution C;

[0048] (3) Add madecassoside, peony root bark extract and moisturizer to the mixed solution C, mix and filter to obtain the composition.

[0049] In some embodiments, the heating in step (2) is heating to 60° C.-70° C. In some embodiments, the heating in step (2) is heating to 60° C., 61° C., 62° C., 63° C., 64° C., 65° C., 66° C., 67° C., 68° C., 69° C. or 70° C.

[0050] In some embodiments, the room temperature is 10° C.-30° C. In some embodiments, the room temperature is 10° C., 11° C., 12° C., 13° C., 14° C., 15° C., 16° C., 17° C., 18° C., 19° C., 20° C., 21° C., 22° C., 23° C., 24° C., 25° C., 26° C., 27° C., 28° C., 29° C., or 30° C.

[0051] In a third aspect, the present invention provides an application of the composition described in the first aspect or the composition prepared by the preparation method described in the second aspect.

[0052] A use of the composition of the first aspect or the composition prepared by the preparation method of the second aspect in preparing a skin care product.

[0053] In some embodiments, the skin care product is used to prevent skin photoaging or anti-skin aging.

[0054] In some embodiments, the skin care product is used for skin anti-oxidation.

[0055] In some embodiments, the skin care product is used to combat skin wrinkles.

[0056] Beneficial Effects

[0057] Compared with the prior art, a certain embodiment of the present invention has at least one of the following beneficial effects:

[0058] (1) The Ganoderma lucidum extract, sandalwood extract, madecassoside and peony root bark extract used in the present invention have unexpected synergistic antioxidant capacity, anti-wrinkle and other effects, which can resist light pollution from the inside of the skin, reduce free radical formation, inhibit inflammation and erythema formation, and resist skin photoaging and skin aging; in addition, the inventors found that deleting or replacing any one or two of the active ingredients in the Ganoderma lucidum extract, sandalwood extract, madecassoside and peony root bark extract with other similar Chinese medicine extract active ingredients cannot achieve the above-mentioned unexpected synergistic antioxidant capacity, anti-wrinkle and other effects, and the present invention is non-obvious.

[0059] (2) Compared with the content ranges of other active components, the weight ratio range or content range of the active components (Ganoderma lucidum extract, sandalwood extract, madecassoside and peony root bark extract) provided by the present invention is conducive to better achieving excellent antioxidant capacity, anti-wrinkle and other effects, and is conducive to obtaining better anti-photoaging and anti-skin aging effects, and has unexpected synergistic technical effects in terms of antioxidant capacity, anti-wrinkle, anti-photoaging and anti-skin aging.

[0060] (3) The composition of the present invention has simple ingredients, low content of each component, low cost, simple preparation method, low skin irritation and high safety.

[0061] Terminology

[0062] In the description of the present invention, “plurality” means two or more than two, unless otherwise clearly and specifically defined.

[0063] The term "room temperature" means ambient temperature, referring to a temperature between about 10°C and about 30°C, or between about 20°C and 30°C, or about 25°C.

[0064] The term "wt%" means percent by mass.

[0065] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0066] In the following, all numbers disclosed herein are approximate, regardless of whether the word "about" or "approximately" is used. The value of each number may vary by 1%, 2%, 5%, 7%, 8%, 10%, 15% or 20%. Whenever a number with a value of N is disclosed, any number with a value of N+ / -1%, N+ / -2%, N+ / -3%, N+ / -5%, N+ / -7%, N+ / -8%, N+ / -10%, N+ / -15% or N+ / -20% will be explicitly disclosed, where "+ / -" means plus or minus. DETAILED DESCRIPTION

[0067] In order to enable those skilled in the art to better understand the technical solution of the present invention, some non-limiting embodiments are further disclosed below to further describe the present invention in detail.

[0068] The reagents used in the present invention can be purchased from the market or prepared by the method described in the present invention.

[0069] The sources of some of the reagents used in the embodiments or comparative examples of the present invention are as follows:

[0070] SOLUBILISANT LRI: contains 54 wt% PPG-26-buteth-26, 36 wt% PEG-40 hydrogenated castor oil and 10 wt% water, purchased from Sensient Cosmetic Technologies.

[0071] Madecassoside (INCI name: MADECASSOSIDE): purchased from Guilin Laiyin Biotechnology Co., Ltd.

[0072] Peony root bark extract (trade name: Gerbex Suffruticosa): contains 0.4 wt % of peony root bark extract (the INCI name of peony root bark extract is PAEONIA SUFFRUTICOSA ROOT BARK EXTRACT), 50 wt % of 1,3-butanediol and 49.6 wt % of water, purchased from Shanghai Jiakai Biotechnology Co., Ltd.

[0073] Ganoderma lucidum extract: Ganoderma lucidum spore oil (reishi spore oil, purchased from Shanghai Ergothioneine Biotechnology Co., Ltd.; Triterpene (triterpenoid) ≥ 30%), INCI name GANODERMA LUCIDUM (MUSHROOM) EXTRACT.

[0074] Sandalwood extract: the trade name is santalwood biofunctional, the manufacturer is Ashland LLC, containing 0.5 wt% of sandalwood extract and 99.5 wt% of octyldodecanol.

[0075] Example 1-Example 3: Preparation of Composition

[0076] Recipe: See Table 1.

[0077] Table 1: Composition formula

[0078]

[0079] Preparation method:

[0080] (1) mixing the red ganoderma extract, the sandalwood extract, the solubilizer and water to obtain a mixed solution A;

[0081] (2) mixing p-hydroxyacetophenone and 1,3-butanediol and heating (at 60°C-70°C, specifically 65°C) until dissolved, cooling to room temperature (10°C-30°C) to obtain a mixed solution B, and mixing the mixed solution B with the mixed solution A to obtain a mixed solution C;

[0082] (3) Add madecassoside, peony root bark extract and moisturizer to the mixed solution C, mix and filter to obtain the composition.

[0083] Comparative Example 1-Comparative Example 4: Single component missing test

[0084] Formulation: A single component deficiency test was conducted based on the formulation of Example 2. The specific formulation is shown in Table 2.

[0085] Table 2: Composition formula

[0086]

[0087] Preparation method: refer to the preparation method of Example 2. In addition, if a certain component is not contained in the formula, the component is not added during the preparation process.

[0088] Comparative Examples 5-8: Investigation of Single Active Components

[0089] Formulation: A single active ingredient was investigated based on the formulation in Example 2. The specific formulation is shown in Table 3.

[0090] Table 3: Composition formula

[0091]

[0092]

[0093] Preparation method: refer to the preparation method of Example 2. In addition, if a certain component is not contained in the formula, the component is not added during the preparation process.

[0094] Comparative Examples 9-14: Two-component missing test

[0095] Formulation: A two-component deficiency test was conducted based on the formulation of Example 2. The specific formulation is shown in Table 4.

[0096] Table 4: Composition formula

[0097]

[0098]

[0099] Preparation method: refer to the preparation method of Example 2. In addition, if a certain component is not contained in the formula, the component is not added during the preparation process.

[0100] Comparative Examples 15-18: Single component incremental investigation formula: A single component incremental investigation test was carried out based on the formula of Example 2. The specific formula is shown in Table 5.

[0101] Table 5: Composition formula

[0102]

[0103] Preparation method: Prepare according to the preparation method of Example 2.

[0104] Comparative Examples 19-21: Compositions with different ratios

[0105] Recipe: See Table 6.

[0106] Table 6: Composition formulas in different ratio ranges

[0107]

[0108]

[0109] Preparation method: Prepare according to the preparation method of Example 2.

[0110] Test Example 1: Antioxidant Capacity Test

[0111] (1) DPPH free radical scavenging ability

[0112] Experimental principle: The ability of the compositions of Examples 1-3 and Comparative Examples 1-21 to scavenge free radicals was determined using the 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) method. DPPH is a stable nitrogen-centered free radical, whose ethanol solution is purple and has a maximum absorption value at 515nm. When an antioxidant is added to the DPPH solution, its lone pair of electrons is paired, and the absorption disappears or weakens, causing the solution color to become lighter, yellow or light yellow, and the absorbance at 515nm becomes smaller, and the degree of change is linearly related to the degree of free radical scavenging. The activity of the antioxidant is reflected by measuring the concentration (EC50) and time (TEC50) of the antioxidant required when half of the DPPH free radicals remain.

[0113] Experimental methods:

[0114] Preparation of DPPH solution: Weigh DPPH and dilute it to 250 mL brown volumetric flask with anhydrous ethanol to prepare 2×10 - 4 mol / L DPPH solution was stored in a refrigerator away from light for future use.

[0115] Specific determination process: Take 4.0mL 2×10 -4 1.0 mL of 95% ethanol and 4.0 mL of 95% ethanol were mixed and the reaction was stable. The absorbance at 515 nm was measured with 95% ethanol as the reference and recorded as A0.

[0116] The sample solution (the composition prepared in Examples 1-3 and Comparative Examples 1-21) was mixed and reacted until it was stable. The absorbance at 515 nm was measured with 95% ethanol as a reference and recorded as Ax. 4.0 mL of 2×10 -4 mol / L DPPH solution and 1.0 mL of the test sample (the composition prepared in Examples 1-3 and Comparative Examples 1-21), after the reaction is stable, the absorbance is measured at 515 nm with 95% ethanol as a reference, and recorded as Ay; the DPPH clearance rate is calculated according to the following calculation formula: DPPH clearance rate calculation (100%) = [1-(Ay-Ax) / A0] × 100%. The results are shown in Table 7.

[0117] (2) ABTS free radical scavenging ability

[0118] Experimental principle: 2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) is a water-soluble free radical initiator as a color developer. ABTS is oxidized by active oxygen to generate a stable blue-green cationic free radical ABTS· + , add the substance to be tested, if the substance contains antioxidant components, the substance will react with ABTS· + The reaction occurs and the reaction system fades, and then ABTS· + The absorbance change was detected at the maximum absorption wavelength (734 nm) and compared with the control standard system containing water-soluble vitamin E (trolox), and the total antioxidant capacity of the tested substance was calculated.

[0119] ABTS + Solution preparation: Prepare ABTS into a 2mmol / L solution with distilled water, take 50mL of 2mmol / L ABTS + The solution was mixed evenly with 200 mL of K2S2O8 aqueous solution (70 mmol / L) and placed in the dark for 8 h to obtain ABTS· + Solution, set aside.

[0120] Specific determination process: ABTS· +The solution was diluted to an absorbance of 1.0; 1 mL of the composition of Example 1-3 and Comparative Example 1-21 was taken respectively, and 1.9 mL of the diluted ABTS· + The solution was mixed and allowed to stand for 10 minutes, and the absorbance at 734 nm was measured by a spectrophotometer. The scavenging rate of ABTS free radicals was calculated according to the following formula: S = (A0-A) / A0×100%, where A0 is ABTS· + The absorbance of the solution, A is ABTS· + The absorbance of the solution after the solution and the sample to be tested are mixed. The results are shown in Table 7.

[0121] (3) Hydroxyl radical (HO·) scavenging ability

[0122] Experimental principle: HO free radical reaction activity is high and unstable, using H2O2 and Fe 2+ The mixture produces HO·, and the addition of salicylic acid improves its stability and produces a colored substance with a maximum absorption value at 515nm. The antioxidant activity of antioxidants is tested by their ability to scavenge HO· free radicals.

[0123] Experimental method: Use distilled water to prepare 8.8mmol / L H2O2 solution, 9.0mmol / L ferrous sulfate solution and 9.0mmol / L salicylic acid solution respectively; add 1mL 8.8mmol / L H2O2 solution, 1mL 9.0mmol / L ferrous sulfate solution and 1mL 9.0mmol / L salicylic acid solution into a colorimetric tube, and then add 2mL of the sample to be tested (Example 1-3 and Comparative Example 1-21), and shake well; take distilled water as a reference, and measure the absorbance of each colorimetric tube at a wavelength of 515nm using a spectrophotometer.

[0124] The HO· free radical scavenging rate was calculated according to the following formula: SA (%) = (A1-A2) / A1×100%, where A1 is the absorbance without adding the sample to be tested, and A2 is the absorbance with adding the sample to be tested. The results are shown in Table 7.

[0125] Table 7: Antioxidant test results of the compositions of Examples 1-3 and Comparative Examples 1-21

[0126]

[0127]

[0128] Analysis of results: By comparing Examples 1-3 with Comparative Examples 1-21, it can be seen that the composition provided by the present invention has a good anti-light aging effect. Within the content range of each active ingredient provided by the present invention, the active ingredients have an unexpected synergistic technical effect.

[0129] (1) By comparing Example 3 with Example 1-Example 2, it can be seen that within the range, as the amount of the composition increases, the anti-photoaging effect increases accordingly.

[0130] (2) The results of Comparative Examples 1 to 14 and Example 2 are evaluated according to the calculation formula of Jin Zhengjun's method or other methods for evaluating synergistic enhancement. It can be seen that the activities of the active ingredients of the composition in DPPH free radical scavenging ability, ABTS free radical scavenging ability, and hydroxyl free radical scavenging ability have excellent synergistic effects.

[0131] (3) Comparison of Comparative Examples 15 to 19 with Example 2 shows that the anti-photoaging efficacy of the examples with a single increase in any active ingredient of the composition decreased, and the unexpected synergistic technical effect could not be achieved. A possible explanation is that a single increase in a certain component destroyed the balance of the amount or concentration required for the synergistic anti-photoaging reaction of each active ingredient through different pathways.

[0132] (4) Comparison of Examples 1 to 3 with Comparative Examples 19 to 21 shows that the content range of each active ingredient provided by this patent is the optimal ratio of the active ingredients in the composition, and compared with other content ranges of the active ingredients, the content range of each active ingredient provided by this patent has an unexpected synergistic technical effect between the active ingredients.

[0133] The above results show that the compositions provided in Examples 1 to 3 have good antioxidant function, can effectively remove free radicals, and can play an anti-skin aging role.

[0134] Test Example 2: Skin elasticity and skin wrinkle test

[0135] 120 healthy female subjects aged between 40 and 60 were selected (divided into 24 groups, 5 people in each group), with visible wrinkles or fine lines at the corners of the eyes. The female subjects fixed half of their faces every day, and used the composition of Example 1-Example 3 or Comparative Example 1-Comparative Example 21 once in the morning and evening, and applied it continuously for 8 weeks. No other brand of cosmetics were used during the test. The test was conducted once at 0, 2, 4, 6 and 8 weeks respectively. Before each test, the subjects were required to clean their faces with clean water, wipe the facial moisture with sterile paper towels, sit and rest for at least half an hour in an environment with a temperature of about 25°C and a relative humidity of about 50%, and then measured by professionals. The skin at the tail of the left and right eyes was measured using a skin elasticity tester (German Courage+Khazaka Company, model: MPA580). The skin elasticity parameter R5 (R5 = Ur / Ue, the ratio of the elastic part of the skin during the recovery process to the elastic part of the negative pressure application process in the first cycle of the skin test, the closer the value is to 1, the better the skin elasticity) and the firming parameter F4 (the smaller the value, the firmer the skin) are used as evaluation indicators to evaluate the effect of anti-wrinkle cosmetics on the improvement of the elasticity of the skin around the eyes. The skin at the left and right eye ends was measured three times each, and the average value was calculated. The experimental results are shown in Table 8.

[0136] The skin of the left and right eye tails of the subjects (subjects in the skin elasticity test process) was photographed by a facial image analyzer (Courage+Khazaka, Germany, model: Visioface) (operated according to the instrument manual), and the wrinkle visualization coefficient reduction rate (%) was calculated to evaluate the improvement of eye wrinkles before and after using anti-wrinkle cosmetics. The experimental results are shown in Table 9.

[0137] Table 8: Skin elasticity test results of the compositions of Examples 1-3 and Comparative Examples 1-21

[0138]

[0139]

[0140] Table 9: Skin wrinkle improvement results of the compositions of Examples 1-3 and Comparative Examples 1-21

[0141]

[0142]

[0143] Analysis of results: By comparing Examples 1-3 with Comparative Examples 1-21, it can be seen that the composition provided by the present invention has a good anti-photoaging effect, and the active ingredients of the composition provided by the present invention have a synergistic effect; the anti-photoaging effect of the examples with a single increase in any active ingredient of the composition decreases, and a possible explanation is that a single increase in a certain active ingredient destroys the balance of the amount or concentration required for the synergistic anti-photoaging reaction of each active ingredient through different pathways; the combination of hydroxymadecassoside, peony root bark extract, red ganoderma extract and sandalwood extract in the composition produces an unexpectedly excellent anti-photoaging effect, and the four plant extracts are indispensable. The obtained composition has multiple beauty and skin care effects, has a synergistic anti-wrinkle effect on skin wrinkles, and makes the skin elastic and shiny.

[0144] Test Example 3: Zebrafish Anti-wrinkle Efficacy Test

[0145] Zebrafish anti-wrinkle efficacy test was conducted on Examples 1-3. Wild-type AB strain zebrafish was used as the test system, and the relative expression levels of three type I collagen genes (collala, collalb and colla2 encoding α1(I), α2(I) and α3(I) chains, respectively) present in zebrafish were detected to indicate whether the sample had anti-wrinkle efficacy.

[0146] Experimental method: Zebrafish 4 days after fertilization were randomly selected in a 6-well plate, with 30 fish in each well; water-soluble samples were given, and a normal control group was set up at the same time, with a capacity of 3 mL per well. Three biological replicates were performed; incubation was carried out at 28°C in the dark for 24 hours; total RNA of zebrafish in each experimental group was extracted, cDNA was synthesized, and the gene expression of β-actin and the target gene was detected by q-PCR; β-actin was used as an internal reference for gene expression to calculate the relative RNA expression of the target gene. (Relative RNA expression = 2ΔΔC(t), where ΔΔC(t) = ΔC(t) normal control group - ΔC(t) sample group, ΔC(t) = C(t) target gene - C(t) β-actin). The results are shown in Table 10.

[0147] Table 10: Zebrafish anti-wrinkle efficacy test results of the compositions of Examples 1-3

[0148]

[0149] Result analysis: Examples 1-3 have excellent anti-aging effects.

[0150] Test Example 4: Skin irritation safety test

[0151] Experimental method: Refer to the human skin patch test in the "2015 Cosmetic Safety Technical Specifications". The method of skin occlusion patch test is: select 30 people aged 18-60 years old, and select an area not exceeding 50mm2 , a qualified spot test device with a depth of about 1mm, take 0.020mL of the composition prepared in the above example and put it into the small chamber of the spot tester, the control well is a blank control (no substance is placed), the spot tester with the composition is applied to the curved side of the forearm of the subject with a low-allergenic tape, and the palm is gently pressed to make it evenly applied to the skin, and it lasts for 24 hours. Observe the skin reaction according to the standards of Table 11 30 minutes (after the indentation disappears), 24 hours and 48 hours after removing the spot tester containing the composition, and record the observation results. The patch test results of the composition are shown in Table 12.

[0152] Table 11: Skin occlusion patch test skin reaction grading standards:

[0153]

[0154] Table 12: Human safety test results of the compositions of Examples 1-3

[0155]

[0156]

[0157] The experimental results show that the compositions prepared in Examples 1-3 passed the human patch safety test.

[0158] The method of the present invention has been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein within the content, spirit and scope of the present invention to implement and apply the technology of the present invention. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters. It is particularly important to point out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention.

Claims

1. A composition, characterized in that The invention comprises madecassoside, peony root bark extract, red ganoderma extract, sandalwood extract, solvent and other auxiliary materials; the other auxiliary materials include solubilizer, moisturizer and preservative; the solvent is water; the solubilizer is SOLUBILISANT LRI; the moisturizer is glycerin; the preservative includes p-hydroxyacetophenone and 1,3-butylene glycol; The peony root bark extract contains 0.2wt%-1.0wt% of peony root bark extract, 40wt%-60wt% of 1,3-butanediol, and the balance is water; The red ganoderma extract is ganoderma spore oil; The sandalwood extract contains 0.4wt%-1.0wt% of sandalwood extract, and the remainder is octyl dodecanol; Calculated based on the total mass of the composition, the content of the hydroxy-madecassoside is 0.10000wt%-0.30000wt%; the content of the peony root bark extract is 0.01000wt%-0.10000wt%; the content of the red ganoderma extract is 0.00010wt%-0.00100wt%; the content of the sandalwood extract is 0.00001wt%-0.00010wt%; the content of the SOLUBILISANT LRI is 0.10000wt%-1.00000wt%; the content of the humectant is 5.00000wt%-15.00000wt%; the content of the hydroxyacetophenone is 0.20000 wt%-0.50000wt%, the content of 1,3-butanediol in the preservative in the composition is 1.00000wt%-5.00000wt%; the balance is water.

2. The composition according to claim 1, characterized in that The peony root bark extract contains 0.4 wt% of peony root bark extract, 50 wt% of 1,3-butanediol and 49.6 wt% of water; The sandalwood extract contains 0.5wt% of sandalwood extract, and the remainder is octyl dodecanol.

3. The composition according to any one of claims 1 to 2, characterized in that The other auxiliary materials include a solubilizer, a moisturizer and a preservative; the moisturizer is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxy-madecassoside is 0.10000wt%; the content of the peony root bark extract is 0.01000wt%; the content of the red ganoderma extract is 0.00010wt%; the content of the sandalwood extract is 0.00001wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the moisturizer is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; the balance is water; or The other auxiliary materials include a solubilizer, a moisturizer and a preservative; the moisturizer is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxy-madecassoside is 0.20000wt%; the content of the peony root bark extract is 0.05000wt%; the content of the red ganoderma extract is 0.00050wt%; the content of the sandalwood extract is 0.00005wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the moisturizer is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; the balance is water; or The other auxiliary materials include a solubilizer, a humectant and a preservative; the humectant is glycerol, and the preservatives are p-hydroxyacetophenone and 1,3-butylene glycol; calculated based on the total mass of the composition, the content of hydroxymadecassoside is 0.30000wt%; the content of the peony root bark extract is 0.10000wt%; the content of the red ganoderma extract is 0.00100wt%; the content of the sandalwood extract is 0.00010wt%; the content of SOLUBILISANT LRI is 0.50000wt%; the content of the humectant is 10.00000wt%; the content of p-hydroxyacetophenone is 0.30000wt%; the content of 1,3-butylene glycol in the preservative in the composition is 2.00000wt%; and the balance is water.

4. The composition according to claim 1, characterized in that The dosage form of the composition is an external dosage form.

5. The composition according to claim 1, characterized in that The composition is in the form of an aqueous solution, a spray or an aerosol.

6. The composition according to claim 1, characterized in that The composition is in the form of an external aqueous solution, an external spray or an external aerosol.

7. A method for preparing the composition according to any one of claims 1 to 6, characterized in that: The other auxiliary materials include solubilizers, moisturizers and preservatives; the preservatives include p-hydroxyacetophenone and 1,3-butanediol; the preparation method includes: (1) mixing the red ganoderma extract, the sandalwood extract, the solubilizer and water to obtain a mixed solution A; (2) mixing p-hydroxyacetophenone and 1,3-butanediol and heating them until dissolved, cooling to room temperature to obtain a mixed solution B, and mixing the mixed solution B with the mixed solution A to obtain a mixed solution C; (3) Adding madecassoside, peony root bark extract and moisturizer to the mixed solution C, mixing and filtering to obtain the composition.

8. Use of the composition according to any one of claims 1 to 6 or the composition prepared by the preparation method according to claim 7 in the preparation of skin care products.

9. The use according to claim 8, characterized in that: The skin care product is used for preventing skin photoaging or resisting skin aging.

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