A composite plant fermentation product and its preparation method, application and daily chemical product

Abstract

The present application belongs to the technical field of cosmetic production, and discloses a composite plant fermentation product, which is obtained by subjecting ganoderma lucidum, glycyrrhiza glabra, knotweed, peony and rhodiola rosea to enzymolysis and yeast fermentation and then filtering, and the mass ratio of ganoderma lucidum, glycyrrhiza glabra, knotweed, peony and rhodiola rosea is 1:1-4:1-7:1-9:1-60. The present application obtains a composite plant fermentation product by fermenting the above plants, and the fermentation product obtains a composite plant fermentation product with good anti-wrinkle, firming and soothing effects by fermenting and screening the functional ingredients in the above plants by yeast. In addition, the present application also discloses a preparation method of the composite plant fermentation product, the use of the composite plant fermentation product and a daily chemical product.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical product production, and in particular to a composite plant fermentation product, a preparation method thereof, an application thereof, and a daily chemical product. Background Art

[0002] Hyaluronic acid, elastin and free radicals are very important factors in skin health and beauty. Their effects on the skin are multifaceted, among which hyaluronic acid is an important component naturally present in human tissues, which plays a role in moisturizing and lubricating the skin. Hyaluronic acid can absorb moisture and maintain the moisture balance of the skin, keeping the skin soft, smooth and elastic. With age and the influence of the external environment, the hyaluronic acid content in the skin will gradually decrease, causing dry skin, sagging and wrinkles. Therefore, proper hyaluronic acid supplementation can effectively improve the problem of dry skin and dehydration, making the skin more youthful and elastic.

[0003] Elastin is a structural protein that is mainly found in the dermis of the skin. It has good elasticity and ductility, and can support and maintain the structure and firmness of the skin. With age and the influence of external factors, the elastin in the skin will gradually decrease, causing the skin to loosen, sag and wrinkle. Therefore, maintaining the elastin content of the skin is essential to maintain the firmness and youthfulness of the skin. Through appropriate skin care products and maintenance methods, it can help promote the synthesis and maintenance of elastin, thereby improving the problem of loose and sagging skin.

[0004] Free radicals are highly reactive oxidative molecules that can cause oxidative damage and aging to the skin. Free radicals mainly come from environmental pollution, ultraviolet radiation, bad living habits and other factors, which can damage the structure and function of skin cells, leading to skin aging, spots, wrinkles and other problems. Therefore, anti-oxidation is an important means to protect the skin from free radical damage. By consuming foods rich in antioxidants, such as fruits, vegetables and nuts, and using skin care products containing antioxidant ingredients, you can help neutralize free radicals and slow down the process of skin aging.

[0005] In summary, hyaluronic acid, elastin and free radicals play a very important role in skin health and beauty. Proper skin care methods and maintenance measures can help maintain the skin's moisture balance, elasticity and antioxidant capacity, so that the skin remains young, healthy and smooth. Therefore, we should pay attention to the impact of these factors on the skin and take effective measures to protect and improve the health of the skin.

[0006] Prior art 1: Chinese patent application 202010260346.0 discloses a cosmetic composition with anti-glycation and anti-aging effects and its application, which belongs to the technical field of functional compositions, including 0.5-4 parts of vitamin C, 0.5-4 parts of SOD yeast powder, 0.2-5 parts of Haematococcus pluvialis, 0.5-10 parts of thread-leaved broom, 0.5-8 parts of licorice extract, 0.1-6 parts of bayberry extract, 0.5-8 parts of longan shell extract, 0.1-8 parts of burdock polyphenols, 0.5-8 parts of cinnamon extract, 0.1-5 parts of ginseng extract, 0.5-8 parts of kudzu root extract, 1-10 parts of purslane extract, 2-40 parts of carbohydrates, 2-30 parts of proteins and 0.5-5 parts of lipids. This solution can improve skin aging caused by skin oxidation, ultraviolet damage, skin glycation, melanin deposition, etc. The invention can prevent aging and darkening of the skin, make the skin in good condition, achieve the effect of delaying aging and whitening, and the application also reasonably mixes various nutritional components of medicine and food such as vitamin C, yeast powder containing SOD, Haematococcus pluvialis, broom, licorice extract, bayberry extract, longan shell extract, burdock polyphenols, cinnamon extract, etc., so that they can synergize with each other, use yeast powder containing SOD as the first line of defense against free radicals, match Haematococcus pluvialis that can penetrate the cell membrane, and cooperate with broom to effectively remove free radicals, enhance the skin's antioxidant defense ability, and fundamentally prevent the accumulation of oxidative stress that causes skin damage and aging;

[0007] Further observation of the scheme shows that although the scheme discloses that Polygonum cuspidatum extract, Ganoderma lucidum extract, Rhodiola rosea extract, Paeonia lactiflora extract, and Glycyrrhiza glabra extract can be used, on the one hand, the above-mentioned plant extracts are not the main components of the scheme, and the common use of the above-mentioned plant extracts is not disclosed in the examples. Therefore, it is impossible to know what effects the Polygonum cuspidatum extract, Ganoderma lucidum extract, Rhodiola rosea extract, Paeonia lactiflora extract, and Glycyrrhiza glabra extract in the scheme can exert when used together. On the other hand, the scheme does not ferment the above-mentioned plant extracts, so the types and contents of the active ingredients in the prepared composition are also different.

[0008] Prior art 2: Chinese patent application 201910615975.8 discloses a composition for whitening, removing freckles, removing acne and improving skin and a preparation method thereof, wherein the composition is prepared by mixing component A, component B, component C, component D, component E, component F, component G and component H; the preparation method comprises the following steps: A, heating and stirring the components in component A to obtain liquid A; heating and stirring the components in component B to obtain liquid B; B, slowly adding liquid B to liquid A, homogenizing and emulsifying once, adding component C, homogenizing and emulsifying twice, adding component D, homogenizing and emulsifying three times to obtain an emulsified liquid, adding component E to the emulsified liquid after cooling, stirring evenly to obtain a mixed liquid; C, adding component F and component G to the mixed liquid, stirring evenly to obtain a repair cream for whitening, removing freckles, removing acne and improving skin; the repair cream prepared by this scheme has the effects of mildly removing acne, brightening skin tone, improving skin roughness, fading fine lines, and intensively repairing skin;

[0009] However, it should be noted that although the scheme includes the use of extracts such as Glycyrrhiza glabra, Paeonia lactiflora, and Polygonum cuspidatum, the scheme also does not use yeast to ferment the above extracts.

[0010] The problem that this program needs to solve is: How to develop a composite plant fermentation product with good anti-wrinkle, firming and soothing abilities. Summary of the invention

[0011] The purpose of the present application is to provide a composite plant fermentation product with good anti-wrinkle, firming and soothing abilities. The present application uses enzymatic hydrolysis and fermentation of multiple plants to increase the content of products with hyaluronidase inhibitory ability, elastase inhibitory ability, and DPPH free radical scavenging ability in the fermentation product, thereby obtaining a fermentation product with good anti-wrinkle, firming and soothing abilities. In addition, the present application also provides a preparation method, application and a daily chemical product of the above-mentioned composite plant fermentation product.

[0012] To achieve the above-mentioned purpose, the present application discloses a composite plant fermentation product, which is obtained by enzymatic hydrolysis and yeast fermentation of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea, and the mass ratio of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 1:1-4:1-7:1-9:1-60.

[0013] Among them, Lingzhi is rich in a variety of antioxidant substances, which can help neutralize free radicals, reduce oxidative damage, delay skin aging, and improve skin resistance. At the same time, Lingzhi has anti-inflammatory effects, which can reduce skin inflammation and allergic reactions, help repair damaged skin, and Lingzhi can increase the moisture content of the skin, improve dry skin problems, and make the skin softer and smoother. The active ingredients in Lingzhi can inhibit the production of melanin, reduce spots and dullness, and make the skin brighter and more even. Lingzhi also has antibacterial effects, which can effectively inhibit bacterial growth and reduce the occurrence of acne and inflammation.

[0014] Licorice root has a strong anti-inflammatory effect, can reduce skin inflammation and allergic reactions, and helps soothe sensitive skin. Licorice root is also rich in antioxidants, which can neutralize free radicals, reduce oxidative damage, and delay skin aging. Licorice root can also increase the moisture content of the skin, improve dry skin, and make the skin more moisturized and soft. Licorice root has a whitening effect, which can inhibit the production of melanin, reduce spots and dullness, and make the skin brighter and more even. Finally, licorice root has a soothing effect on the skin, which helps to reduce skin discomfort and redness, and makes the skin more comfortable and calm.

[0015] Polygonum cuspidatum has strong antioxidant properties, which can neutralize free radicals, slow down the skin aging process, reduce the appearance of fine lines and wrinkles, and make the skin younger and healthier. Polygonum cuspidatum has anti-inflammatory effects, which can reduce skin inflammation and allergic reactions, help soothe sensitive skin, reduce redness and itching, and inhibit the production of melanin, reduce spots and dullness, make the skin brighter and more even, and have a whitening effect. Polygonum cuspidatum also helps to increase the moisture content of the skin, improve dry skin, keep the skin well hydrated, softer and more moisturized. Finally, Polygonum cuspidatum has a certain antibacterial effect, which can help reduce bacterial growth and prevent acne and other skin problems.

[0016] Peony has a good anti-inflammatory effect, which can help reduce skin inflammation and allergic reactions, soothe sensitive skin, reduce redness and itching, and help soothe the skin, relieve skin discomfort and tightness, and make the skin more comfortable and calm. At the same time, peony has an astringent effect, which can help shrink pores, improve skin sagging and roughness, and make the skin more delicate and firm. Peony is also rich in a variety of antioxidants, which can neutralize free radicals, reduce oxidative damage, delay skin aging, and keep the skin young and healthy. Finally, peony can increase the moisture content of the skin, improve dry skin, and make the skin more moisturized and soft.

[0017] Rhodiola rosea is rich in natural antioxidants, which can neutralize free radicals, reduce oxidative damage, slow down the skin aging process, and make the skin younger and healthier. At the same time, Rhodiola rosea has a good anti-inflammatory effect, which can reduce skin inflammation and allergic reactions, help soothe sensitive skin, reduce redness and itching, and help repair damaged skin, promote skin cell regeneration, accelerate wound healing, and make the skin smoother and healthier. Rhodiola rosea can also increase the moisture content of the skin, improve dry skin, and keep the skin hydrated and soft. Finally, Rhodiola rosea has a certain antibacterial effect, which can help reduce bacterial growth and prevent acne and other skin problems.

[0018] Furthermore, the mass ratio of the ganoderma lucidum, licorice root, polygonum cuspidatum, peony root and rhodiola rosea is 1:2-4:3-7:3-9:5-30.

[0019] Furthermore, the yeast is selected from at least one of galactose yeast, sake yeast, and brewer's yeast.

[0020] Furthermore, the yeast at least comprises galactosaccharomyces galactosidase.

[0021] Furthermore, the yeast is a mixture of galactose yeast, sake yeast and brewing yeast, and the mass ratio of the galactose yeast, sake yeast and brewing yeast is 0.5-2:1:1.

[0022] In addition, the present application also discloses a preparation method for the above-mentioned composite plant fermentation product, which comprises mixing Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea in a mass ratio of 1:1-4:1-7:1-9:1-60, crushing, enzymolysis, yeast fermentation and then filtering to obtain the composite plant fermentation product.

[0023] Furthermore, the preparation method of the composite plant composition is specifically as follows: Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea in a mass ratio of 1:2-4:3-7:3-9:5-30 are mixed, crushed and enzymolyzed to obtain an enzymolysis solution, and then the enzymolysis solution is mixed with peptone, glucose, sodium dihydrogen phosphate and pure water to obtain a culture medium, and finally yeast is inoculated into the culture medium for fermentation, and after fermentation, the bacterial cells are crushed, and the supernatant is obtained by centrifugation to obtain a composite plant fermentation product;

[0024] During the enzymatic hydrolysis process, the total mass ratio of enzyme to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea was 1 to 3:100;

[0025] During the fermentation process, the total mass ratio of yeast to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 6-8:100.

[0026] Furthermore, the enzyme used in the enzymatic hydrolysis process is selected from at least one of neutral protease, cellulosic protease and papain.

[0027] In addition, the present application also discloses the use of the above-mentioned composite plant fermentation product in preparing daily chemical products.

[0028] In addition, the present application also discloses a daily chemical product containing 0.1 to 100 wt % of the composite plant fermentation product as described above, wherein the dosage form of the daily chemical product is a patch, an ointment, a liquid, a spray, a gel, an emulsion, or a cream.

[0029] The beneficial effects of the present application are as follows: the ganoderma lucidum in the present application is rich in a variety of antioxidant substances, which can help neutralize free radicals, reduce oxidative damage, delay skin aging, and improve skin resistance. At the same time, ganoderma lucidum has an anti-inflammatory effect, which can reduce skin inflammation and allergic reactions, and help repair damaged skin. Ganoderma lucidum can increase the moisture content of the skin, improve dry skin problems, and make the skin softer and smoother. The active ingredients in ganoderma lucidum can inhibit the production of melanin, reduce spots and dullness, and make the skin brighter and more even. Ganoderma lucidum also has an antibacterial effect, which can effectively inhibit the growth of bacteria and reduce the occurrence of acne and inflammation.

[0030] Licorice root has a strong anti-inflammatory effect, can reduce skin inflammation and allergic reactions, and helps soothe sensitive skin. Licorice root is also rich in antioxidants, which can neutralize free radicals, reduce oxidative damage, and delay skin aging. Licorice root can also increase the moisture content of the skin, improve dry skin, and make the skin more moisturized and soft. Licorice root has a whitening effect, which can inhibit the production of melanin, reduce spots and dullness, and make the skin brighter and more even. Finally, licorice root has a soothing effect on the skin, which helps to reduce skin discomfort and redness, and makes the skin more comfortable and calm.

[0031] Polygonum cuspidatum has strong antioxidant properties, which can neutralize free radicals, slow down the skin aging process, reduce the appearance of fine lines and wrinkles, and make the skin younger and healthier. Polygonum cuspidatum has anti-inflammatory effects, which can reduce skin inflammation and allergic reactions, help soothe sensitive skin, reduce redness and itching, and inhibit the production of melanin, reduce spots and dullness, make the skin brighter and more even, and have a whitening effect. Polygonum cuspidatum also helps to increase the moisture content of the skin, improve dry skin, keep the skin well hydrated, softer and more moisturized. Finally, Polygonum cuspidatum has a certain antibacterial effect, which can help reduce bacterial growth and prevent acne and other skin problems.

[0032] Peony has a good anti-inflammatory effect, which can help reduce skin inflammation and allergic reactions, soothe sensitive skin, reduce redness and itching, and help soothe the skin, relieve skin discomfort and tightness, and make the skin more comfortable and calm. At the same time, peony has an astringent effect, which can help shrink pores, improve skin sagging and roughness, and make the skin more delicate and firm. Peony is also rich in a variety of antioxidants, which can neutralize free radicals, reduce oxidative damage, delay skin aging, and keep the skin young and healthy. Finally, peony can increase the moisture content of the skin, improve dry skin, and make the skin more moisturized and soft.

[0033] Rhodiola rosea is rich in natural antioxidants, which can neutralize free radicals, reduce oxidative damage, slow down the skin aging process, and make the skin younger and healthier. At the same time, Rhodiola rosea has a good anti-inflammatory effect, which can reduce skin inflammation and allergic reactions, help soothe sensitive skin, reduce redness and itching, and help repair damaged skin, promote skin cell regeneration, accelerate wound healing, and make the skin smoother and healthier. Rhodiola rosea can also increase the moisture content of the skin, improve dry skin, and keep the skin hydrated and soft. Finally, Rhodiola rosea has a certain antibacterial effect, which can help reduce bacterial growth and prevent acne and other skin problems.

[0034] In addition, the present application obtains a composite plant composition by fermenting the above-mentioned plants. The composition has good anti-wrinkle, firming and soothing effects through the fermentation and screening of effective ingredients in the above-mentioned plants by yeast. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a schematic diagram of a chicken embryo before the composition of Example 1 is added dropwise;

[0036] Figure 2 This is a schematic diagram of a chicken embryo after the composition of Example 1 is added dropwise. DETAILED DESCRIPTION

[0037] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0038] Before presenting the embodiments and comparative examples, the raw materials involved in the embodiments and comparative examples are described as follows, as shown in Table 1:

[0039] Table 1

[0040]

[0041]

[0042] Example 1

[0043] A preparation method of a composite plant fermentation product comprises mixing and crushing ganoderma lucidum, licorice root, knotweed, peony root and rhodiola rosea in a mass ratio of 1:3:5:7:10, then adding pure water to boil and keeping warm for 1 hour, then performing enzymolysis after cooling to obtain an enzymolysis solution, then mixing the enzymolysis solution with peptone, glucose, sodium dihydrogen phosphate and pure water to obtain a culture medium, wherein the mass fraction of the enzymolysis solution is 80%, the mass fraction of peptone is 2%, the mass fraction of glucose is 2%, the mass fraction of sodium dihydrogen phosphate is 1.5%, and pure water is added to make the total mass fraction 100%;

[0044] Then, the yeast is inoculated into the culture medium for fermentation, and after fermentation, the bacteria are broken, and the supernatant is taken by centrifugation to obtain a composite plant fermentation product;

[0045] The enzyme used in the enzymatic hydrolysis process is neutral protease, and the yeast is galactosyl yeast;

[0046] The total mass ratio of the enzyme to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea was 2:100;

[0047] The total mass ratio of yeast to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 7:100.

[0048] Example 2

[0049] A preparation method of a composite plant fermentation product comprises mixing and crushing ganoderma lucidum, licorice root, knotweed, peony root and rhodiola rosea in a mass ratio of 1:2:7:9:30, then adding pure water to boil and keeping warm for 1 hour, then performing enzymolysis after cooling to obtain an enzymolysis solution, then mixing the enzymolysis solution with peptone, glucose, sodium dihydrogen phosphate and pure water to obtain a culture medium, wherein the mass fraction of the enzymolysis solution is 80%, the mass fraction of peptone is 2%, the mass fraction of glucose is 2%, the mass fraction of sodium dihydrogen phosphate is 1.5%, and pure water is added to make the total mass fraction 100%;

[0050] Then, the yeast is inoculated into the culture medium for fermentation, and after fermentation, the bacteria are broken, and the supernatant is taken by centrifugation to obtain a composite plant fermentation product;

[0051] The enzyme used in the enzymatic hydrolysis process is neutral protease, and the yeast is galactosyl yeast;

[0052] The total mass ratio of the enzyme to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea was 1:100;

[0053] The total mass ratio of yeast to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 8:100.

[0054] Example 3

[0055] A preparation method of a composite plant fermentation product comprises mixing and crushing ganoderma lucidum, licorice root, knotweed, peony root and rhodiola rosea in a mass ratio of 1:4:3:3:5, then adding pure water, boiling and keeping warm for 1 hour, cooling and performing enzymolysis to obtain an enzymolysis solution, then mixing the enzymolysis solution with peptone, glucose, sodium dihydrogen phosphate and pure water to obtain a culture medium, wherein the mass fraction of the enzymolysis solution is 80%, the mass fraction of peptone is 2%, the mass fraction of glucose is 2%, the mass fraction of sodium dihydrogen phosphate is 1.5%, and pure water is added to make the total mass fraction 100%;

[0056] Then, the yeast is inoculated into the culture medium for fermentation, and after fermentation, the bacteria are broken, and the supernatant is taken by centrifugation to obtain a composite plant fermentation product;

[0057] The enzyme used in the enzymatic hydrolysis process is neutral protease, and the yeast is galactosyl yeast;

[0058] The total mass ratio of the enzyme to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea was 3:100;

[0059] The total mass ratio of yeast to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 6:100.

[0060] Example 4

[0061] The method is basically the same as Example 1, except that the mass ratio of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 1:3:6:8:20.

[0062] Example 5

[0063] The method is basically the same as Example 1, except that the enzyme is a mixed enzyme of papain and cellulose protease, the mass ratio of papain to cellulose protease is 1:1, and the total amount of enzyme added remains unchanged.

[0064] Example 6

[0065] The method is basically the same as Example 1, except that the enzyme is a mixed enzyme of neutral protease, papain and cellulose protease, and the mass ratio of neutral protease, papain and cellulose protease is 1:1:1, and the total amount of enzyme added remains unchanged.

[0066] Example 7

[0067] The method is basically the same as Example 1, except that the yeast is sake yeast and the total amount of yeast added remains unchanged.

[0068] Example 8

[0069] The method is basically the same as Example 1, except that the yeast is a mixed yeast of galactose yeast and sake yeast, the mass ratio of galactose yeast to sake yeast is 1:1, and the total amount of yeast added remains unchanged.

[0070] Example 9

[0071] The same as Example 1, except that the yeast is

[0072] A mixed yeast of brewer's yeast and sake yeast, with the mass ratio of brewer's yeast to sake yeast being 1:1, and the total amount of yeast added remaining unchanged.

[0073] Example 10

[0074] The method is basically the same as Example 6, except that the yeast is a mixed yeast of galactose yeast, sake yeast and brewer's yeast, and the mass ratio of galactose yeast, sake yeast and brewer's yeast is 1:1:1, and the total amount of yeast added remains unchanged.

[0075] Embodiment 11

[0076] The method is basically the same as Example 1, except that the yeast is a mixed yeast of galactose yeast, sake yeast and brewer's yeast, and the mass ratio of galactose yeast, sake yeast and brewer's yeast is 1:1:1, and the total amount of yeast added remains unchanged.

[0077] Comparative Example 1

[0078] The method is basically the same as Example 1, except that when the total mass is the same, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea are replaced with Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea in a mass ratio of 3:5:7:10.

[0079] Comparative Example 2

[0080] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea in a mass ratio of 1:5:7:10 are used to replace Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea.

[0081] Comparative Example 3

[0082] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Paeonia lactiflora, and Rhodiola rosea are replaced by Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea in a mass ratio of 1:3:7:10.

[0083] Comparative Example 4

[0084] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, and Rhodiola rosea in a mass ratio of 1:3:5:10 are used to replace Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea.

[0085] Comparative Example 5

[0086] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, and Paeonia lactiflora in a mass ratio of 1:3:5:7 are used to replace Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea.

[0087] Comparative Example 6

[0088] The method is basically the same as Example 1, except that when the total mass is the same, angelica, licorice, knotweed, peony and rhodiola rosea in a mass ratio of 1:3:5:7:10 are used to replace ganoderma lucidum, licorice, knotweed, peony and rhodiola rosea.

[0089] Comparative Example 7

[0090] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Gentiana scabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea in a mass ratio of 1:3:5:7:10 are used to replace Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea.

[0091] Comparative Example 8

[0092] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Polygonatum sibiricum, Paeonia lactiflora, and Rhodiola rosea are replaced by Ganoderma lucidum, Glycyrrhiza glabra, Polygonatum cuspidatum, Paeonia lactiflora, and Rhodiola rosea in a mass ratio of 1:3:5:7:10.

[0093] Comparative Example 9

[0094] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Red ginseng and Rhodiola rosea in a mass ratio of 1:3:5:7:10 are used to replace Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea.

[0095] Comparative Example 10

[0096] The method is basically the same as Example 1, except that when the total mass is the same, Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Euryale ferox are replaced by Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea in a mass ratio of 1:3:5:7:10.

[0097] Performance Test:

[0098] 1. Irritation test

[0099] 1.1 Instruments and Equipment

[0100] Fully automatic incubator, stereo microscope, SPF chicken embryos;

[0101] 1.2 Reagents

[0102] Sodium chloride, sodium dodecyl sulfate (SDS);

[0103] 1.3 Incubation conditions

[0104] Room temperature is 20℃~25℃, relative humidity is 45%~70%, incubation temperature is 37.5℃±0.5℃, relative humidity is 55%~70%, turntable is 3 times / h~6 times / h, and it is not necessary to rotate when incubating 9-day-old chicken embryos.

[0105] 1.4 Test methods

[0106] (1) Test operation steps

[0107] In this test, 6 embryos were selected for each group, and the conditions of the chorioallantoic membrane were recorded with a camera, and a polytetrafluoroethylene resin ring was placed on the chorioallantoic membrane of the chicken embryo, and photographed and recorded. The composite plant fermentation products of the embodiments and comparative examples with a concentration of 100% were used as the test samples, and then the test samples were added to the polytetrafluoroethylene resin ring, the time of adding the samples was recorded, and the air chamber was covered with a moistened plastic wrap, and the chicken embryos were moved to a constant temperature and humidity chamber for culture, and the degree of change of each toxic effect was observed.

[0108] (2) Observation of results

[0109] Bleeding, coagulation, and vascular melting were observed and recorded, and scored according to their severity.

[0110] The scoring criteria for bleeding, coagulation, and vascular lysis are shown in Table 2:

[0111] Table 2

[0112]

[0113] Rating criteria: ES≤12: no / mild irritation; 12<ES<16: moderate irritation; ES≥16: strong irritation.

[0114] According to the test, the embodiments and comparative examples did not produce irritation. Figure 1-2 , is a comparison of the states of chicken embryos before and after the composition of Example 1 is added dropwise to chicken embryos. According to statistics, the ES value of the solution prepared by the composition of Example 1 is 2.33.

[0115] 2. Hyaluronidase inhibition rate test

[0116] 2.1 Instruments and Equipment

[0117] BSA224S analytical balance, L6s UV spectrophotometer

[0118] 2.2 Reagents

[0119] Hyaluronidase, BR, Sodium Hyaluronate, BR

[0120] 2.3 Test methods

[0121] (1) Treatment of control and test samples

[0122] Sample group: diluted with pure water to a sample concentration of 10%;

[0123] Negative control: pure water.

[0124] (2) Test operation steps

[0125] Set up a sample group and a negative control group. Each group needs to have 3 parallels. Add the corresponding reagent solution to each group, shake well, and leave it at room temperature for 30 minutes for color development. Use an ultraviolet spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0126] (3) The calculation formula is shown in Formula 1:

[0127]

[0128] Where: A—is the absorbance of the reaction solution without sample;

[0129] B—is the absorbance of the reaction solution without sample and enzyme;

[0130] C—is the absorbance of the reaction solution containing sample and enzyme;

[0131] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0132] (4) Data analysis

[0133] The statistical analysis software was SPSS, and the comparison between the hyaluronidase inhibition rate of the test sample and the negative control was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of a = 0.05. P ≥ 0.05 indicated that there was no significant difference between the two groups; P < 0.05 indicated that there was a significant difference between the two groups.

[0134] The test results of hyaluronidase inhibition rate of Examples 1-10 and Comparative Examples 1-10 are shown in Table 3:

[0135] Table 3

[0136]

[0137] Result analysis:

[0138] 1. It can be seen from Examples 1-4 that when the mass ratios of various plants (Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, Rhodiola rosea) are slightly changed, although the hyaluronic acid inhibition rates of Examples 1-4 fluctuate, the fluctuations are relatively small;

[0139] 2. It can be seen from Examples 1 and 5-6 that when a mixed enzyme of papain and cellulose protease is used to replace neutral protease, the hyaluronic acid inhibition rate of Example 5 decreases to a certain extent relative to Example 1, but the decline is not obvious. At the same time, it can be seen from Example 6 that when neutral protease, papain and cellulose protease are compounded, Example 6 increases by nearly 3% relative to Example 1. It can be seen that when neutral protease, papain and cellulose protease are compounded, the increase in the inhibition rate of hyaluronidase has a significant upward trend;

[0140] 3. It can be seen from Examples 1 and 7-10 that when galactose yeast is replaced with sake yeast or a mixture of brewer's yeast and sake yeast, the hyaluronidase inhibition rates of Examples 7 and 9 are reduced to varying degrees. We believe that the number of fermentation products that have an inhibitory effect on hyaluronidase produced by galactose yeast during the fermentation process is greater or the effect is more excellent;

[0141] At the same time, further observation of Examples 1 and 6 and 10 shows that when galactose yeast is replaced by a mixed yeast of galactose yeast, sake yeast and brewer's yeast and the plant is enzymatically hydrolyzed using a mixed enzyme of neutral protease, papain and cellulose protease, the hyaluronidase inhibition rate is not only improved relative to Example 1, but also nearly 2% relative to Example 6. It can be seen that when the three yeasts are used at the same time, the quantity or quality of the fermentation product with hyaluronidase inhibition ability obtained by fermentation also has a certain degree of improvement trend. And through the cases of using galactose yeast, brewer's yeast and sake yeast mixed yeast in Examples 1 and 9 respectively, it can be seen that the theoretical hyaluronidase inhibition rate of Example 10 using the three yeasts together should be within 46±1%, while the actual hyaluronidase inhibition rate of Example 10 reached 49.549%. It is believed that the reason for this phenomenon may be that, on the one hand, when the three yeasts are used simultaneously, the quantity or quality of the fermentation product with the hyaluronidase inhibition rate is improved, but since the performance of Examples 7-9 is not as good as that of Example 1, the above situation has a certain possibility, but it is more likely that when the mixed yeast and the mixed enzyme are used together, a synergistic effect is produced between the two, and the enzymatic hydrolysis of multiple enzymes increases the content of fermentation raw materials that are easy to be brewed by sake yeast and brewer's yeast in the enzymatic hydrolysis liquid, thereby increasing the overall fermentation capacity of the mixed yeast. It can be seen from Example 11 that when only the yeasts are mixed, the hyaluronidase inhibition ability of the fermentation product is still lower than that of Example 1, while the hyaluronidase inhibition ability of the fermentation product of Example 10 is higher than that of Example 6, which further proves that the hyaluronidase inhibition ability of the fermentation product can be effectively improved when the enzyme and yeast are used simultaneously.

[0142] 4. It can be seen from Example 1 and Comparative Examples 1-5 that when any plant component is missing from Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Peony, and Rhodiola rosea, the inhibition rate of hyaluronidase has a significant downward trend. It can be seen that any plant component from Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Peony, and Rhodiola rosea is indispensable in this solution;

[0143] 5. It can be seen from Example 1 and Comparative Examples 1-5 that when any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is replaced by other plants, the inhibition rate of hyaluronidase shows a significant downward trend. It can be seen that in this solution, any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is irreplaceable.

[0144] 3. Elastase inhibition test

[0145] 3.1 Instruments and Equipment

[0146] BSA224S analytical balance, RT-6100 ELISA analyzer;

[0147] 3.2 Reagents

[0148] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0149] 3.3 Test methods

[0150] (1) Treatment of control and test samples

[0151] Sample group: diluted with pure water to a sample concentration of 10%;

[0152] Negative control: pure water.

[0153] (2) Test operation steps

[0154] Set up a sample group and a negative control group, and set up 3 parallels for each group. Add the corresponding reagent solution into a 96-well plate, shake it gently, incubate it at 25°C for 15 minutes, place it in an enzyme reader, and measure the absorbance at 410nm.

[0155] (3) The calculation formula is shown in Formula 2:

[0156]

[0157] Where: A—is the absorbance of the reaction solution without sample;

[0158] B—is the absorbance of the reaction solution without sample and enzyme;

[0159] C—is the absorbance of the reaction solution containing sample and enzyme;

[0160] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0161] (4) Data analysis

[0162] The statistical analysis software was SPSS, and the comparison of the elastase inhibition rate between the test sample, positive control and negative control was performed by independent sample t test. The above statistical analysis was two-tailed test, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0163] The test results of elastase inhibition rate of Examples 1-11 and Comparative Examples 1-10 are shown in Table 4:

[0164] Table 4

[0165]

[0166]

[0167] Result analysis:

[0168] 1. It can be seen from Examples 1-4 that when the mass ratios of various plants (Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, Rhodiola rosea) are slightly changed, although the elastase inhibition rates of Examples 1-4 fluctuate, the fluctuations are relatively small;

[0169] 2. It can be seen from Examples 1 and 5-6 that when a mixed enzyme of papain and cellulose protease or a mixed enzyme of neutral protease, papain and cellulose protease is used to replace the neutral protease, the inhibition rate of the single protease in Examples 5 and 6 shows a significant downward trend relative to that in Example 1. It can be seen that in the application scenario of promoting the quality or quantity of products with elastase inhibition ability, the neutral protease has a more obvious advantage;

[0170] 3. It can be seen from Examples 1 and 7-10 that when galactose yeast is replaced by sake yeast, a mixed yeast of galactose yeast and sake yeast, or a mixed yeast of brewing yeast and sake yeast, the elastase inhibition rates of Examples 7-9 all show a decreasing trend to varying degrees. It can be seen that in this scheme, the product with elastase inhibition ability obtained by fermentation with galactose yeast alone is more advantageous in quality or quantity than the product with elastase inhibition ability obtained by the mixed yeast described in the schemes of Examples 7-9;

[0171] At the same time, when Example 10 was enzymolyzed using the same mixed enzyme as Example 6, its elastase inhibition rate showed a significant growth trend relative to Example 6. It can be seen that when the three yeasts were used in combination, it was more advantageous than using galactose yeast alone. We believe that although galactose yeast was more advantageous than sake yeast and brewer's yeast in the process of fermenting and producing products with elastase inhibition ability, when the three were compounded, sake yeast and brewer's yeast may reduce the fermentation difficulty of galactose yeast on the enzymatic hydrolyzate, such as galactose yeast using the fermentation intermediates of sake yeast and brewer's yeast to improve the quality or quantity of products with elastase inhibition ability, thereby enabling Example 10 to achieve a more excellent elastase inhibition rate relative to Example 6;

[0172] 4. It can be seen from Example 1 and Comparative Examples 1-5 that when any of the plant components of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea are missing, the inhibition rate of elastase has a significant downward trend. It can be seen that any of the plant components of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea are indispensable in this solution;

[0173] 5. It can be seen from Example 1 and Comparative Examples 1-5 that when any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is replaced by other plants, the inhibition rate of elastase shows a significant downward trend. It can be seen that in this solution, any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is irreplaceable.

[0174] 4.DPPH free radical scavenging rate test

[0175] 4.1 Instruments and Equipment

[0176] BSA224S analytical balance, L6s UV spectrophotometer

[0177] 4.2 Reagents

[0178] DPPH (1,1-diphenyl-2-picrylhydrazyl), 98%

[0179] 4.3 Test methods

[0180] (1) Treatment of control and test samples

[0181] Sample group: diluted with pure water to a sample concentration of 10%;

[0182] Negative control: pure water.

[0183] (2) Test operation steps

[0184] Set up a sample group and a negative control group. Set up 3 parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let it stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1cm

[0185] The absorbance was measured at 517 nm in a cuvette.

[0186] (3) The calculation formula is shown in Formula 3:

[0187]

[0188] Where: T—absorbance of sample tube, i.e. absorbance of solution after sample reacts with DPPH;

[0189] T0—sample background absorbance;

[0190] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution when no sample is added;

[0191] C0—Solvent background absorbance.

[0192] (4) Data analysis

[0193] The statistical analysis software was SPSS, and the comparison of DPPH free radical scavenging rates among the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0194] The DPPH free radical scavenging rate test results of Examples 1-10 and Comparative Examples 1-10 are shown in Table 5:

[0195] Table 5

[0196]

[0197]

[0198] Result analysis:

[0199] 1. It can be seen from Examples 1-4 that when the mass ratios of various plants (Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, Rhodiola rosea) are slightly changed, although the DPPH free radical scavenging rates of Examples 1-4 fluctuate, the fluctuation range is relatively small;

[0200] 2. It can be seen from Examples 1 and 5-6 that when the neutral protease is replaced by a mixed enzyme of papain and cellulase, the DPPH radical scavenging rate of Example 5 shows a certain downward trend relative to Example 1. It can be seen that in the production process of products with DPPH radical scavenging ability, the neutral protease plays a leading role. Further observation of Example 6 shows that when the neutral protease is replaced by a mixed enzyme of neutral protease, papain and cellulase, the DPPH radical scavenging rate of Example 6 relative to Example 1 has a certain upward trend. It can be seen that although the neutral protease plays a leading role in the production of products with DPPH radical scavenging ability, when it is used together with papain and cellulase, the DPPH radical scavenging ability of the composite plant fermentation product is further improved. We believe that when the three enzymes are used simultaneously, papain and cellulase promote the improvement of the enzymatic hydrolysis ability of the neutral protease on plants, thereby leading to the improvement of the DPPH radical scavenging ability of the composite plant fermentation product;

[0201] 3. It can be seen from Examples 1 and 7-9 that when galactose yeast is replaced by sake yeast, a mixed yeast of galactose yeast and sake yeast, and a mixed yeast of brewing yeast and sake yeast, the composite plant fermentation products obtained in Examples 7-9 all show different degrees of decline, which shows that galactose yeast plays a major role in the production of products with DPPH free radical scavenging ability;

[0202] At the same time, it can be seen from Example 10 that when a mixed yeast of galactose yeast, sake yeast and brewer's yeast is used instead of galactose yeast, the DPPH free radical scavenging rate of Example 10 shows a significant upward trend relative to that of Example 6.

[0203] 4. It can be seen from Example 1 and Comparative Examples 1-5 that when any plant component is missing from Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Peony, and Rhodiola rosea, the DPPH free radical scavenging rate has a significant downward trend. It can be seen that any plant component from Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Peony, and Rhodiola rosea is indispensable in this solution;

[0204] 5. It can be seen from Example 1 and Comparative Examples 1-5 that when any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is replaced by other plants, the DPPH free radical scavenging rate shows a significant downward trend. It can be seen that in this solution, any plant component of Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea is irreplaceable.

[0205] Application Examples

[0206] A composite plant fermentation product essence, the formula ratio of which is shown in Table 6.

[0207] Table 6

[0208]

Claims

1. A composite plant fermentation product, characterized in that: The ganoderma lucidum, glycyrrhiza glabra, knotweed, peony and rhodiola rosea are obtained by enzymolysis and yeast fermentation and then filtration, wherein the mass ratio of ganoderma lucidum, glycyrrhiza glabra, knotweed, peony and rhodiola rosea is 1:1-4:1-7:1-9:1-60; The enzyme used in the enzymolysis process is selected from at least one of neutral protease, cellulase and papain; The yeast used in the yeast fermentation process is selected from at least one of galactose yeast, sake yeast and brewer's yeast.

2. The composite plant fermentation product according to claim 1, characterized in that: The mass ratio of the ganoderma lucidum, licorice root, polygonum cuspidatum, peony root and rhodiola rosea is 1:2-4:3-7:3-9:5-30.

3. The composite plant fermentation product according to claim 1, characterized in that: The yeast contains at least Galactomyces galactosus.

4. The composite plant fermentation product according to claim 1, characterized in that: The yeast is a mixture of galactose yeast, sake yeast and brewing yeast, and the mass ratio of the galactose yeast, sake yeast and brewing yeast is 0.5-2:1:

1.

5. A method for preparing the composite plant fermentation product according to any one of claims 1 to 4, characterized in that: The composite plant fermentation product is obtained by mixing ganoderma lucidum, licorice root, knotweed, peony root and rhodiola rosea in a mass ratio of 1:1-4:1-7:1-9:1-60, crushing, enzymolysis, yeast fermentation and filtering.

6. The method for preparing the composite plant fermentation product according to claim 5, characterized in that: The preparation method of the composite plant composition is specifically as follows: Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea in a mass ratio of 1:2-4:3-7:3-9:5-30 are mixed, crushed and enzymolyzed to obtain an enzymolyzate, then the enzymolyzate is mixed with peptone, glucose, sodium dihydrogen phosphate and pure water to obtain a culture medium, finally yeast is inoculated into the culture medium for fermentation, the bacterial body is crushed after fermentation, and the supernatant is obtained by centrifugation to obtain a composite plant fermentation product; During the enzymatic hydrolysis process, the total mass ratio of enzyme to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora, and Rhodiola rosea was 1 to 3:100; During the fermentation process, the total mass ratio of yeast to Ganoderma lucidum, Glycyrrhiza glabra, Polygonum cuspidatum, Paeonia lactiflora and Rhodiola rosea is 6-8:

100.

7. Use of the composite plant fermentation product according to any one of claims 1 to 4 in preparing daily chemical products.

8. A daily chemical product, characterized in that: The daily chemical product contains 0.1 to 100 wt % of the composite plant fermentation product according to any one of claims 1 to 4, and the dosage form of the daily chemical product is a patch, an ointment, a liquid, a spray, a gel, an emulsion, or a cream.

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