A fully automatic enzyme-linked immunosorbent assay device and method thereof

Abstract

The invention discloses a fully automatic enzyme-linked immunosorbent spot detection device and a method thereof, belonging to the technical field of detection equipment. The detection equipment comprises a detection equipment body, wherein a fixed platform is fixedly connected between inner walls on both sides of the detection equipment body; and a cleaning mechanism, wherein the cleaning mechanism comprises a cleaning component, a drying component, a supporting component, four groups of rotating components, multiple groups of fixed components and multiple power components. The cleaning component, the drying component and the supporting component are all arranged on the fixed platform, a pipette head is fixed by adjusting a fixing clamp, and then the pipette head is adjusted to move to a suitable position, the pipette head is cleaned by a fixed motor and a transmission, and is dried and disinfected by a blower and a disinfection lamp, thereby improving work efficiency, and multiple fixing mechanisms are fixed to suitable positions by fixing an adjusting sliding plate to a fixing groove, and multiple test tube racks of various types can be fixed by a fixing plate, thereby increasing their practicality.

Description

Technical Field

[0001] The present invention belongs to the technical field of detection equipment, and in particular relates to a fully automatic enzyme-linked immunosorbent spot detection device and a method thereof. Background Art

[0002] The fully automatic ELISpot detection equipment is a device that can automatically perform ELISpot test analysis. It captures the spots formed on the PVDF membrane by cytokines secreted by cells, counts and analyzes the spots, and thus evaluates the functional status of cells. It is mainly used for immunological research at the cellular level.

[0003] In actual use, the existing automatic ELISA spot detection equipment is unable to clean the pipette tip, and the pipette tip needs to be cleaned additionally before reuse, which leads to poor working efficiency. At the same time, some existing fully automatic ELISA spot detection equipment, in actual use, is installed with multiple test tube racks of different specifications, and their positions cannot be fixed, resulting in poor practicality. Summary of the invention

[0004] The purpose of the present invention is to provide a fully automatic enzyme-linked immunosorbent spot detection device and method thereof, which fixes the pipette head by adjusting two fixing clamps, turns on the rotating motor to drive the moving box to move on the threaded rod through transmission, drives the pipette head to move to a suitable position on the installation tube, and pushes the cleaning box to move to a suitable position by installing an electric telescopic rod, so that the pipette head is located in the cleaning box, and then turns on the fixing motor, the fixing motor rotates, and the installation tube drives the pipette head to rotate through transmission to clean the pipette head, and restores the cleaning box to its original state by installing the electric telescopic rod, and blows air through the blower, and connects The pipette head is air-dried by the connecting pipe and the air-drying plate. The pipette head can be dried and disinfected by the disinfection lamp. The pipette head can be cleaned and replaced to improve work efficiency. The mounting plate and the sliding plate are inserted into the appropriate positions in the fixing groove, and then the mounting plate is pressed and the fixing plate is rotated to move the fixing bolts in the mounting plate and the threaded barrel. The fixing bolts squeeze the moving rod. When the fixing bolts make the moving rod push the sliding plate to move on the fixing rod until the sliding plate is fixed to the fixing groove, multiple fixing mechanisms can be fixed to appropriate positions. Multiple test tube racks of various types can be fixed by the fixing plate, thereby increasing its practicality.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A fully automatic enzyme-linked immunosorbent spot detection device comprises: a detection device body, wherein a fixed platform is fixedly connected between the inner walls on both sides of the detection device body; a cleaning mechanism, wherein the cleaning mechanism comprises a cleaning component, a drying component, a supporting component, four groups of rotating components, multiple groups of fixed components and multiple power components, wherein the cleaning component, the drying component and the supporting component are all arranged on the fixed platform, wherein each group of the rotating components and the power components are arranged on the supporting component, and wherein each group of the fixed components are arranged on the power component; and a fixing mechanism, wherein the fixing mechanism comprises multiple fixing grooves, a moving component and two groups of engaging components, wherein the multiple fixing grooves are all opened on the outer surface of the fixed platform, wherein the moving component is slidably arranged between the inner walls on both sides of the fixing groove, and wherein each group of the engaging components is arranged on the moving component.

[0007] Among them, the cleaning component includes a water tank, a transmission pump, a wastewater tank, a transmission pipe, a plurality of installed electric telescopic rods and four cleaning boxes, the plurality of installed electric telescopic rods are fixedly connected to the lower inner wall of the fixed platform, the bottom of each of the cleaning boxes is respectively fixedly connected to the extended ends of the plurality of installed electric telescopic rods, the two ends of the transmission pipe are respectively fixedly connected between the water tank and the four cleaning boxes, the tops of the water tank and the wastewater tank are both fixedly connected to the bottom of the fixed platform, the transmission pump is fixedly connected to the bottom of the fixed platform, and the input end of the transmission pump is connected to the four cleaning boxes through a water pipe, and the output end of the transmission pump is connected to the wastewater tank.

[0008] Among them, the drying component includes four blowers, multiple connecting pipes, multiple disinfection lamps and multiple air-drying plates. Each of the blowers is fixedly connected to the lower inner wall of the fixed platform, one end of the multiple connecting pipes is fixedly connected to the output ends of the four blowers respectively, the bottom of each air-drying plate is fixedly connected to the top of the connecting pipe, and the multiple disinfection lamps are fixedly connected to the outer surface of one side of the air-drying plate.

[0009] Wherein, the supporting components include two connecting plates, four installation boxes, multiple installation tubes and multiple fixed covers, the top of each connecting plate is fixedly connected to the inner wall of the fixed platform, the two ends of each installation box are fixedly connected between the two connecting plates, each installation tube rotates and passes through the bottom of the installation box, and the bottom of each fixed cover is fixedly connected to the top of the installation tube.

[0010] Among them, each group of rotating parts includes a fixed motor, two synchronous wheels, a transmission rod, four fixed gears, four mounting rods, four connecting gears and eight fixed bevel gears. The transmission rod is rotatably connected between the front and rear inner walls of the mounting box, each mounting rod is rotatably connected to the lower inner wall of the mounting box, each fixed gear is fixedly sleeved on the outer surface of the mounting rod, wherein four fixed bevel gears are respectively fixedly sleeved on the outer surfaces of four mounting rods, and another four fixed bevel gears are fixedly sleeved on the outer surface of the transmission rod, every two fixed bevel gears are meshed with each other, each connecting gear is fixedly sleeved on the outer surface of the mounting tube, and each connecting gear is meshed with the fixed gear, the fixed motor is fixedly connected to the lower inner wall of the mounting box, one of the synchronous wheels is fixedly sleeved on the output end of the fixed motor, and the other synchronous wheel is fixedly sleeved on the outer surface of the transmission rod, and the two synchronous wheels are mutually transmitted through a synchronous belt.

[0011] Among them, each group of the power components includes a rotating motor, a rotating gear, a linkage gear, two mounting gears, two threaded rods and two moving boxes. The rotating motor is fixedly connected to the inner surface wall on one side of the fixed cover, the rotating gear is fixedly sleeved on the output end of the rotating motor, the linkage gear is rotatably embedded in the inner surface wall on one side of the fixed cover, and the linkage gear and the rotating gear are meshed with each other, each of the threaded rods is rotatably connected between the upper and lower inner walls of the mounting tube, each of the mounting gears is fixedly sleeved on the outer surface of the threaded rod, the two mounting gears are meshed with the linkage gears, each of the moving boxes is slidably embedded between the inner walls on both sides of the mounting tube, and each moving box is threadedly sleeved on the outer surface of the threaded rod.

[0012] Among them, each group of the fixed components includes two fixed electric telescopic rods, a fixing clamp, two sliding boxes, two limit rods and four support rods, one end of each of the fixed electric telescopic rods is fixedly connected to the outer surface of one side of the mobile box, the outer surface of one side of the fixing clamp is fixedly connected to the output ends of the two fixed electric telescopic rods, each of the support rods is fixedly connected between the upper and lower inner walls of the mounting tube, each of the sliding boxes is slidably embedded between the inner walls on both sides of the mounting tube, each of the sliding boxes is slidably sleeved between the two support rods, each of the limit rods is fixedly connected between the inner walls on both sides of the sliding box, and both end surfaces of each of the fixing clamps are slidably sleeved on the outer surface of the limit rod.

[0013] Among them, the moving part includes a fixing plate, a mounting plate, a fixing bolt and a threaded barrel, the bottom of the fixing plate is fixedly connected to the top of the fixing bolt, the top of the threaded barrel is fixedly connected to the bottom of the mounting plate, and the bottom end thread of the fixing bolt passes through the mounting plate and the threaded barrel.

[0014] Among them, each group of the engaging parts includes a moving rod, a sliding plate, a fixed rod and a fixed spring, the fixed rod is fixedly connected between the inner walls of both sides of the mounting plate, the sliding plate is slidably sleeved on the outer surface of the fixed rod, the fixed spring is sleeved on the outer surface of the fixed rod, and the fixed spring is fixedly connected between the sliding plate and the mounting plate, one end of the moving rod is fixedly connected to the outer surface of one side of the sliding plate, and the other end of the moving rod slides through the outer surface of the threaded cylinder.

[0015] A fully automatic enzyme-linked immunosorbent assay method comprises the following steps:

[0016] Step 1: Fix the detection device: insert the mounting plate and the sliding plate into the fixing groove, then press the mounting plate and rotate the fixing plate to move the fixing bolts in the mounting plate and the threaded barrel. The fixing bolts move to squeeze the moving rods, so that the moving rods push the sliding plate to move on the fixing rods until the sliding plate is fixed to the fixing groove. Multiple fixing mechanisms are fixed in the fixing grooves, and various types of detectors can be fixed on the fixing table.

[0017] Step 2, fix the pipette head: since the detection equipment needs to replace a large number of pipette heads, place the pipette head to be cleaned in the installation tube, and then turn on the fixed electric telescopic rod, the fixed electric telescopic rod pushes the fixed clamp to move, and the two fixed clamps move on the two limit rods respectively, so that the two fixed clamps fix the pipette head;

[0018] Step 3, cleaning the pipette head: turn on the rotating motor, the rotating motor drives the rotating gear to rotate, the rotating gear and the linkage gear are meshed to make the linkage gear rotate, the linkage gear and the mounting gear are meshed to make the mounting gear and the threaded rod rotate, the moving box moves on the threaded rod, the sliding box moves on the support rod, until the fixed clamp drives the pipette head to move on the mounting tube, the cleaning box is pushed to move by installing the electric telescopic rod, so that the pipette head is located in the cleaning box, and then turn on the fixed motor, the fixed motor drives one of the synchronous wheels to rotate, through the synchronous belt transmission, the other synchronous wheel drives the transmission rod to rotate, through the meshing between the two fixed bevel gears, the mounting rod drives the fixed gear to rotate, through the meshing of the fixed gear and the connecting gear, the mounting tube drives the pipette head to rotate, the pipette head is cleaned, the water in the water storage tank enters the cleaning box through the transmission pipe through the transmission pump, and the waste water enters the waste water tank through the transmission pump;

[0019] Step 4. Dry the pipette head: restore the cleaning box to its original state by installing an electric telescopic rod, blow air with a blower, dry the pipette head through the connecting pipe and the air-drying plate, and dry and disinfect the pipette head through the disinfection lamp.

[0020] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0021] (1) In the present invention, the pipette head is fixed by adjusting two fixing clamps, and the rotating motor is turned on to drive the moving box to move on the threaded rod through transmission, driving the pipette head to move to a suitable position on the mounting tube. The cleaning box can be pushed to move to a suitable position by installing an electric telescopic rod, so that the pipette head is located in the cleaning box. Then, the fixed motor is turned on and rotated. Through transmission, the mounting tube drives the pipette head to rotate to clean the pipette head. The cleaning box is restored to its original state by installing an electric telescopic rod. Air can be blown by a blower, and the pipette head can be air-dried by a connecting pipe and an air-drying plate. The pipette head is dried and disinfected by a disinfection lamp. The pipette head can be cleaned and replaced, thereby improving work efficiency.

[0022] (2) In the present invention, by inserting the mounting plate and the sliding plate into the appropriate positions in the fixing groove, and then pressing the mounting plate and rotating the fixing plate, the fixing bolt moves in the mounting plate and the threaded tube, and the fixing bolt squeezes the moving rod. When the fixing bolt causes the moving rod to push the sliding plate to move on the fixing rod until the sliding plate is fixed to the fixing groove, multiple fixing mechanisms can be fixed to appropriate positions, and various types of detection equipment that need to be used can be fixed by the fixing plate, thereby increasing its practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a front perspective view of the present invention;

[0024] Figure 2 is a side perspective cross-sectional view of the present invention;

[0025] Figure 3 It is a front perspective view of the cleaning mechanism part of the present invention;

[0026] Figure 4 It is a frontal perspective cross-sectional view of a cleaning mechanism portion of the present invention;

[0027] Figure 5 A side perspective cross-sectional view of the cleaning mechanism of the present invention;

[0028] Figure 6 It is an enlarged view of part A of the present invention;

[0029] Figure 7 It is a front perspective cross-sectional view of a portion of the cleaning mechanism of the present invention;

[0030] Figure 8 A side perspective cross-sectional view of a portion of the cleaning mechanism of the present invention;

[0031] Fig. 9 It is a front perspective view of the fixing mechanism of the present invention;

[0032] Fig.10 It is a side perspective partial cross-sectional view of the fixing mechanism of the present invention.

[0033] Markings in the figure:

[0034] 1. Fixing table; 2. Testing equipment body; 3. Cleaning mechanism; 301. Water storage tank; 302. Transmission pump; 303. Wastewater tank; 304. Transmission pipe; 305. Cleaning box; 306. Install electric telescopic rod; 307. Connecting plate; 308. Installing box; 309. Blower; 310. Connecting pipe; 311. Air drying plate; 312. Disinfection lamp; 313. Installing cylinder; 314. Fixing cover; 315. Installing rod; 316. Transmission rod; 317. Fixing bevel gear; 318. Fixing motor; 319. Synchronous wheel; 320. Connecting gear; 321, rotating motor; 322, rotating gear; 323, installing gear; 324, threaded rod; 325, moving box; 326, fixing electric telescopic rod; 327, fixing clamp; 328, sliding box; 329, limiting rod; 330, supporting rod; 331, fixing gear; 332, linkage gear; 4, fixing mechanism; 401, fixing groove; 402, fixing plate; 403, installing plate; 404, threaded cylinder; 405, fixing spring; 406, fixing rod; 407, sliding plate; 408, moving rod; 409, fixing bolt. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0036] Reference Figure 1-Figure 10 :The present invention provides a technical solution:

[0037] A fully automatic enzyme-linked immunosorbent spot detection device comprises: a detection device body 2, wherein a fixed platform 1 is fixedly connected between the inner walls on both sides of the detection device body 2; a cleaning mechanism 3, wherein the cleaning mechanism 3 comprises a cleaning component, a drying component, a supporting component, four groups of rotating components, multiple groups of fixed components and multiple power components, wherein the cleaning component, the drying component and the supporting component are all arranged on the fixed platform 1, wherein each group of rotating components and the power components are arranged on the supporting component, and each group of fixed components are arranged on the power component; and a fixing mechanism 4, wherein the fixing mechanism 4 comprises multiple fixing grooves 401, a moving component and two groups of engaging components, wherein the multiple fixing grooves 401 are all opened on the outer surface of the fixed platform 1, wherein the moving component is slidably arranged between the inner walls on both sides of the fixing grooves 401, and wherein each group of engaging components is arranged on the moving component.

[0038] In this embodiment: the detection device body 2 is used to detect enzyme-linked immunosorbent spots, the cleaning mechanism 3 is used to clean the pipette tip, the cleaning component is used to clean the pipette tip, the drying component is used to dry the pipette tip, the supporting component is used to support the rotating component, four groups of rotating components are used to adjust the position of the fixed component, multiple groups of fixed components fix the pipette head, multiple power components are used to provide rotational force, the fixing mechanism 4 is used to fix the detection equipment, multiple fixing grooves 401 are used to fix the moving component, the moving component is used to adjust the position of the two groups of fixed components, and the fixed component is used to fix the detection equipment.

[0039] Specifically, the cleaning components include a water tank 301, a transmission pump 302, a wastewater tank 303, a transmission pipe 304, a plurality of installed electric telescopic rods 306 and four cleaning boxes 305. The plurality of installed electric telescopic rods 306 are fixedly connected to the lower inner wall of the fixed platform 1, and the bottom of each cleaning box 305 is respectively fixedly connected to the extended ends of the plurality of installed electric telescopic rods 306. The two ends of the transmission pipe 304 are respectively fixedly connected between the water tank 301 and the four cleaning boxes 305. The tops of the water tank 301 and the wastewater tank 303 are both fixedly connected to the bottom of the fixed platform 1. The transmission pump 302 is fixedly connected to the bottom of the fixed platform 1, and the input end of the transmission pump 302 is connected to the four cleaning boxes 305 through a water pipe, and the output end of the transmission pump 302 is connected to the wastewater tank 303.

[0040] In this embodiment: the water tank 301 is used to store water, the transmission pump 302 is used to transmit water, the wastewater tank 303 is used to collect wastewater, the transmission pipe 304 is used to transmit water, and multiple electric telescopic rods 306 are installed to adjust the position of the cleaning box 305. The four cleaning boxes 305 are used to store water and clean the pipette heads. The structure and principle of the transmission pump 302 and the electric telescopic rod 306 belong to the prior art and will not be introduced in detail here. The model can be selected according to actual usage.

[0041] Specifically, the drying component includes four blowers 309, multiple connecting pipes 310, multiple disinfection lamps 312 and multiple air-drying plates 311. Each blower 309 is fixedly connected to the lower inner wall of the fixed platform 1, one end of the multiple connecting pipes 310 is fixedly connected to the output ends of the four blowers 309, the bottom of each air-drying plate 311 is fixedly connected to the top of the connecting pipe 310, and the multiple disinfection lamps 312 are fixedly connected to the outer surface of one side of the air-drying plate 311.

[0042] In this embodiment: four blowers 309 are used for blowing air, multiple connecting pipes 310 are used for transmitting air, multiple disinfection lamps 312 are used for disinfection, and multiple air-drying plates 311 are provided for transmitting air. The structure and principle of the blowers 309 and the disinfection lamps 312 belong to the prior art and will not be introduced in detail here. The models can be selected according to actual usage.

[0043] Specifically, the supporting components include two connecting plates 307, four installation boxes 308, multiple installation cylinders 313 and multiple fixed covers 314. The top of each connecting plate 307 is fixedly connected to the inner wall of the fixed platform 1, both ends of each installation box 308 are fixedly connected between the two connecting plates 307, each installation cylinder 313 rotates and passes through the bottom of the installation box 308, and the bottom of each fixed cover 314 is fixedly connected to the top of the installation cylinder 313.

[0044] In this embodiment, two connecting plates 307 and four installation boxes 308 are used to support multiple installation cylinders 313 and multiple fixing covers 314 .

[0045] Specifically, each set of rotating parts includes a fixed motor 318, two synchronous wheels 319, a transmission rod 316, four fixed gears 331, four mounting rods 315, four connecting gears 320 and eight fixed bevel gears 317. The transmission rod 316 is rotatably connected between the front and rear inner walls of the mounting box 308. Each mounting rod 315 is rotatably connected to the lower inner wall of the mounting box 308. Each fixed gear 331 is fixedly sleeved on the outer surface of the mounting rod 315. The four fixed bevel gears 317 are respectively fixedly sleeved on the outer surfaces of the four mounting rods 315. The other four Each fixed bevel gear 317 is fixedly sleeved on the outer surface of the transmission rod 316, and every two fixed bevel gears 317 are meshed with each other. Each connecting gear 320 is fixedly sleeved on the outer surface of the mounting tube 313, and each connecting gear 320 is meshed with the fixed gear 331. The fixed motor 318 is fixedly connected to the lower inner wall of the mounting box 308, one of the synchronous wheels 319 is fixedly sleeved on the output end of the fixed motor 318, and the other synchronous wheel 319 is fixedly sleeved on the outer surface of the transmission rod 316. The two synchronous wheels 319 are mutually transmitted through a synchronous belt.

[0046] In this embodiment: the fixed motor 318 is used to provide rotational force, two synchronous wheels 319, a transmission rod 316, four fixed gears 331, four mounting rods 315, four connecting gears 320 and eight fixed bevel gears 317 are used for transmission, the mounting rod 315 is used to install the fixed gear 331, the structure and principle of the fixed motor 318 belong to the prior art and will not be introduced in detail here, and its model can be selected according to actual usage.

[0047] Specifically, each group of power components includes a rotating motor 321, a rotating gear 322, a linkage gear 332, two mounting gears 323, two threaded rods 324 and two moving boxes 325. The rotating motor 321 is fixedly connected to the inner wall of one side of the fixed cover 314, the rotating gear 322 is fixedly sleeved on the output end of the rotating motor 321, the linkage gear 332 is rotatably embedded in the inner wall of one side of the fixed cover 314, and the linkage gear 332 and the rotating gear 322 are meshed with each other, each threaded rod 324 is rotatably connected between the upper and lower inner walls of the mounting tube 313, each mounting gear 323 is fixedly sleeved on the outer surface of the threaded rod 324, the two mounting gears 323 are meshed with the linkage gear 332, each moving box 325 is slidably embedded between the inner walls on both sides of the mounting tube 313, and each moving box 325 is threadedly sleeved on the outer surface of the threaded rod 324.

[0048] In this embodiment: the rotating motor 321 is used to provide rotating force, the rotating gear 322, the linkage gear 332 and the two mounting gears 323 are used for transmission, the two threaded rods 324 are used to adjust the position of the moving box 325, and the two moving boxes 325 are used to install fixed components. The structure and principle of the rotating motor 321 belong to the prior art and will not be introduced in detail here. Its model can be selected according to actual usage.

[0049] Specifically, each group of fixed components includes two fixed electric telescopic rods 326, a fixed clamp 327, two sliding boxes 328, two limit rods 329 and four support rods 330. One end of each fixed electric telescopic rod 326 is fixedly connected to the outer surface of one side of the mobile box 325, and the outer surface of one side of the fixed clamp 327 is fixedly connected to the output ends of the two fixed electric telescopic rods 326. Each support rod 330 is fixedly connected between the upper and lower inner walls of the mounting tube 313. Each sliding box 328 is slidably embedded between the inner walls on both sides of the mounting tube 313. Each sliding box 328 is slidably sleeved between the two support rods 330. Each limit rod 329 is fixedly connected between the inner walls on both sides of the sliding box 328. Both end surfaces of each fixed clamp 327 are slidably sleeved on the outer surface of the limit rod 329.

[0050] In this embodiment: two fixed electric telescopic rods 326 are used to adjust the position of the fixed clamp 327, the fixed clamp 327 is used to fix the pipette head, two sliding boxes 328 and two limit rods 329 are used to support the fixed clamp 327, and four support rods 330 are used to support the sliding box 328. The structure and principle of the fixed electric telescopic rod 326 belong to the prior art and will not be introduced in detail here. The model can be selected according to actual usage.

[0051] Specifically, the moving parts include a fixed plate 402, a mounting plate 403, a fixing bolt 409 and a threaded tube 404. The bottom of the fixed plate 402 is fixedly connected to the top of the fixing bolt 409, the top of the threaded tube 404 is fixedly connected to the bottom of the mounting plate 403, and the bottom end thread of the fixing bolt 409 passes through the mounting plate 403 and the threaded tube 404.

[0052] In this embodiment: the fixing plate 402 is used to limit the test tube rack, the mounting plate 403 is used to support the fixing plate 402, and the fixing bolt 409 and the threaded barrel 404 cooperate to move the fixing bolt 409.

[0053] Specifically, each set of engaging parts includes a moving rod 408, a sliding plate 407, a fixed rod 406 and a fixed spring 405. The fixed rod 406 is fixedly connected between the inner walls on both sides of the mounting plate 403. The sliding plate 407 is slidably mounted on the outer surface of the fixed rod 406. The fixed spring 405 is mounted on the outer surface of the fixed rod 406, and the fixed spring 405 is fixedly connected between the sliding plate 407 and the mounting plate 403. One end of the moving rod 408 is fixedly connected to the outer surface of one side of the sliding plate 407, and the other end of the moving rod 408 slides through the outer surface of the threaded tube 404.

[0054] In this embodiment: the fixed rod 406 is used to support the sliding plate 407, the sliding plate 407 is used to fix the fixed plate 402 on the fixed platform 1, the moving rod 408 is used to slide in the threaded cylinder 404, the moving rod 408 can push the sliding plate 407 to move, and the fixed spring 405 restores the sliding plate 407 to its original state.

[0055] The following is a detailed description of a fully automatic ELISA spot detection method provided by an embodiment of the present invention, wherein the detection method comprises the following steps:

[0056] Step 1, fix the detection device: insert the mounting plate 403 and the sliding plate 407 into the fixing groove 401, then press the mounting plate 403, rotate the fixing plate 402, so that the fixing bolt 409 moves in the mounting plate 403 and the threaded cylinder 404, and the fixing bolt 409 moves to squeeze the moving rod 408, so that the moving rod 408 pushes the sliding plate 407 to move on the fixing rod 406 until the sliding plate 407 is fixed to the fixing groove 401, and multiple fixing mechanisms 4 are fixed in the fixing groove 401, so that various types of detectors can be fixed on the fixing platform 1;

[0057] Step 2, fixing the pipette head: since the detection equipment needs to replace a large number of pipette heads, the pipette head to be cleaned is placed in the installation tube 313, and then the fixed electric telescopic rod 326 is turned on, and the fixed electric telescopic rod 326 pushes the fixed clamp 327 to move, and the two fixed clamps 327 move on the two limit rods 329 respectively, so that the two fixed clamps 327 fix the pipette head;

[0058] Step 3: Clean the pipette head: Turn on the rotating motor 321, the rotating motor 321 drives the rotating gear 322 to rotate, the rotating gear 322 is meshed with the linkage gear 332, the linkage gear 332 is rotated, the linkage gear 332 is meshed with the installation gear 323, the installation gear 323 and the threaded rod 324 are rotated, the moving box 325 moves on the threaded rod 324, the sliding box 328 moves on the support rod 330, until the fixing clamp 327 drives the pipette head to move on the installation cylinder 313, the cleaning box 305 is pushed to move by installing the electric telescopic rod 306, so that the pipette head is located in the cleaning box 305 Then, the fixed motor 318 is turned on, and the fixed motor 318 drives one of the synchronous wheels 319 to rotate. Through the synchronous belt transmission, the other synchronous wheel 319 drives the transmission rod 316 to rotate. Through the meshing between the two fixed bevel gears 317, the installation rod 315 drives the fixed gear 331 to rotate. Through the meshing of the fixed gear 331 and the connecting gear 320, the installation cylinder 313 drives the pipette head to rotate, and the pipette head is cleaned. The water in the water storage tank 301 enters the cleaning tank 305 through the transmission pipe 304 through the transmission pump 302, and the waste water enters the waste water tank 303 through the transmission pump 302;

[0059] Step 4, drying the pipette tips: restore the cleaning box 305 to its original state by installing the electric telescopic rod 306, blow air through the blower 309, air-dry the pipette tips through the connecting pipe 310 and the air-drying plate 311, and dry and disinfect the pipette tips through the disinfection lamp 312.

[0060] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fully automatic ELISA spot detection device, characterized in that: include: A detection device body (2), wherein a fixing platform (1) is fixedly connected between inner walls on both sides of the detection device body (2); A cleaning mechanism (3), the cleaning mechanism (3) comprising a cleaning component, a drying component, a supporting component, four groups of rotating components, a plurality of groups of fixed components and a plurality of power components, the cleaning component, the drying component and the supporting component are all arranged on a fixed platform (1), each group of the rotating components and the power components are arranged on the supporting component, and each group of the fixed components are arranged on the power component; and a fixing mechanism (4), the fixing mechanism (4) comprising a plurality of fixed grooves (401), a moving component and two groups of engaging components, the plurality of fixed grooves (401) are all opened on the outer surface of the fixed platform (1), the moving component is slidably arranged between the inner walls on both sides of the fixed grooves (401), and each group of the engaging components is arranged on the moving component; The supporting component comprises two connecting plates (307), four installation boxes (308), a plurality of installation cylinders (313) and a plurality of fixed covers (314); the top of each connecting plate (307) is fixedly connected to the upper inner wall of the fixing platform (1); both ends of each installation box (308) are fixedly connected between the two connecting plates (307); each installation cylinder (313) is rotatably inserted through the bottom of the installation box (308); and the bottom of each fixed cover (314) is fixedly connected to the top of the installation cylinder (313); Each group of rotating components comprises a fixed motor (318), two synchronous wheels (319), a transmission rod (316), four fixed gears (331), four mounting rods (315), four connecting gears (320) and eight fixed bevel gears (317); the transmission rod (316) is rotatably connected between the front and rear inner walls of the mounting box (308); each mounting rod (315) is rotatably connected to the lower inner wall of the mounting box (308); each fixed gear (331) is fixedly sleeved on the outer surface of the mounting rod (315); four fixed bevel gears (317) are respectively fixedly sleeved on the outer surfaces of the four mounting rods (315); and the other four connecting gears (320) are fixedly sleeved on the outer surfaces of the four mounting rods (315). The fixed bevel gears (317) are fixedly sleeved on the outer surface of the transmission rod (316), and every two of the fixed bevel gears (317) are meshed with each other. Each of the connecting gears (320) is fixedly sleeved on the outer surface of the mounting tube (313), and each of the connecting gears (320) and the fixed gear (331) are meshed with each other. The fixed motor (318) is fixedly connected to the lower inner wall of the mounting box (308), one of the synchronous wheels (319) is fixedly sleeved on the output end of the fixed motor (318), and the other synchronous wheel (319) is fixedly sleeved on the outer surface of the transmission rod (316), and the two synchronous wheels (319) are mutually driven by a synchronous belt. Each group of the power components comprises a rotating motor (321), a rotating gear (322), a linkage gear (332), two mounting gears (323), two threaded rods (324) and two moving boxes (325); the rotating motor (321) is fixedly connected to an inner surface wall of one side of the fixed cover (314); the rotating gear (322) is fixedly sleeved on an output end of the rotating motor (321); the linkage gear (332) is rotatably embedded in an inner surface wall of one side of the fixed cover (314); and the linkage gear (332) The mounting tube (313) is provided with a plurality of movable boxes (325) which are meshed with the rotating gear (322); each of the threaded rods (324) is rotatably connected between the upper and lower inner walls of the mounting tube (313); each of the mounting gears (323) is fixedly sleeved on the outer surface of the threaded rod (324); the two mounting gears (323) are meshed with the linkage gear (332); each of the movable boxes (325) is slidably embedded between the inner walls on both sides of the mounting tube (313); and each of the movable boxes (325) is threadedly sleeved on the outer surface of the threaded rod (324).

2. A fully automatic ELISA spot detection device as claimed in claim 1, characterized in that: The cleaning component comprises a water storage tank (301), a transmission pump (302), a waste water tank (303), a transmission pipe (304), a plurality of electric telescopic rods (306) and four cleaning boxes (305). The plurality of electric telescopic rods (306) are fixedly connected to the lower inner wall of the fixed platform (1). The bottom of each cleaning box (305) is respectively fixedly connected to the extended end of the plurality of electric telescopic rods (306). Both ends of the transmission pipe (304) are respectively fixedly connected between the water storage tank (301) and the four cleaning boxes (305). The tops of the water storage tank (301) and the waste water tank (303) are both fixedly connected to the bottom of the fixed platform (1). The transmission pump (302) is fixedly connected to the bottom of the fixed platform (1). The input end of the transmission pump (302) is connected to the four cleaning boxes (305) through a water pipe, and the output end of the transmission pump (302) is connected to the waste water tank (303).

3. A fully automatic ELISA spot detection device as claimed in claim 2, characterized in that: The drying component includes four blowers (309), a plurality of connecting tubes (310), a plurality of disinfection lamps (312) and a plurality of air-drying plates (311), each of the blowers (309) is fixedly connected to the lower inner wall of the fixed platform (1), one end of the plurality of connecting tubes (310) is fixedly connected to the output ends of the four blowers (309), the bottom of each air-drying plate (311) is fixedly connected to the top of the connecting tube (310), and the plurality of disinfection lamps (312) are fixedly connected to the outer surface of one side of the air-drying plate (311).

4. A fully automatic ELISA spot detection device as claimed in claim 3, characterized in that: Each group of the fixed components comprises two fixed electric telescopic rods (326), a fixing clamp (327), two sliding boxes (328), two limiting rods (329) and four support rods (330); one end of each fixed electric telescopic rod (326) is fixedly connected to an outer surface of one side of the moving box (325); an outer surface of one side of the fixing clamp (327) is fixedly connected to the output ends of the two fixed electric telescopic rods (326); each support rod (330) is fixedly connected between the upper and lower inner walls of the mounting tube (313); each sliding box (328) is slidably embedded between the inner walls of both sides of the mounting tube (313); each sliding box (328) is slidably sleeved between the two support rods (330); each limiting rod (329) is fixedly connected between the inner walls of both sides of the sliding box (328); and both end surfaces of each fixing clamp (327) are slidably sleeved on the outer surface of the limiting rod (329).

5. A fully automatic ELISA spot detection device as claimed in claim 4, characterized in that: The movable component comprises a fixing plate (402), a mounting plate (403), a fixing bolt (409) and a threaded barrel (404); the bottom of the fixing plate (402) is fixedly connected to the top of the fixing bolt (409); the top of the threaded barrel (404) is fixedly connected to the bottom of the mounting plate (403); and the bottom thread of the fixing bolt (409) penetrates the mounting plate (403) and the threaded barrel (404).

6. A fully automatic ELISA spot detection device as claimed in claim 5, characterized in that: Each group of the engaging components comprises a moving rod (408), a sliding plate (407), a fixed rod (406) and a fixed spring (405); the fixed rod (406) is fixedly connected between the inner walls of both sides of the mounting plate (403); the sliding plate (407) is slidably sleeved on the outer surface of the fixed rod (406); the fixed spring (405) is sleeved on the outer surface of the fixed rod (406); and the fixed spring (405) is fixedly connected between the sliding plate (407) and the mounting plate (403); one end of the moving rod (408) is fixedly connected to the outer surface of one side of the sliding plate (407); and the other end of the moving rod (408) slides through the outer surface of the threaded tube (404).

7. A fully automatic ELISA spot detection method, applied to a fully automatic ELISA spot detection device as claimed in claim 6, characterized in that: The following steps are involved: S1. Fixing the detection device: inserting the mounting plate (403) and the sliding plate (407) into the fixing groove (401), then pressing the mounting plate (403), rotating the fixing plate (402), so that the fixing bolt (409) moves in the mounting plate (403) and the threaded cylinder (404), and the fixing bolt (409) moves to squeeze the moving rod (408), so that the moving rod (408) pushes the sliding plate (407) to move on the fixing rod (406), until the sliding plate (407) is fixed to the fixing groove (401), and fixing a plurality of fixing mechanisms (4) in the fixing groove (401), so that various types of detectors can be fixed on the fixing table (1); S2, fixing the pipette head: since the detection equipment needs to replace a large number of pipette heads, the pipette head to be cleaned is placed in the installation tube (313), and then the fixed electric telescopic rod (326) is turned on, and the fixed electric telescopic rod (326) pushes the fixed clamp (327) to move, and the two fixed clamps (327) move on the two limit rods (329) respectively, so that the two fixed clamps (327) fix the pipette head; S3, cleaning the pipette head: turning on the rotating motor (321), the rotating motor (321) drives the rotating gear (322) to rotate, the rotating gear (322) and the linkage gear (332) are meshed, the linkage gear (332) is rotated, the linkage gear (332) and the installation gear (323) are meshed, the installation gear (323) and the threaded rod (324) are rotated, the moving box (325) moves on the threaded rod (324), the sliding box (328) moves on the support rod (330), until the fixing clamp (327) drives the pipette head to move on the installation cylinder (313), the cleaning box (305) is pushed to move by installing the electric telescopic rod (306), so that the pipette head is located in the cleaning box (305). Then, the fixed motor (318) is turned on, and the fixed motor (318) drives one of the synchronous wheels (319) to rotate. The other synchronous wheel (319) drives the transmission rod (316) to rotate through the synchronous belt transmission. The two fixed bevel gears (317) are meshed, so that the installation rod (315) drives the fixed gear (331) to rotate. The fixed gear (331) and the connecting gear (320) are meshed, so that the installation cylinder (313) drives the pipette head to rotate, and the pipette head is cleaned. The water in the water storage tank (301) enters the cleaning tank (305) through the transmission pipe (304) through the transmission pump (302), and the waste water enters the waste water tank (303) through the transmission pump (302); S4, drying the pipette tip: the cleaning box (305) is restored to its original state by installing the electric telescopic rod (306), air is blown by the blower (309), the pipette tip is air-dried through the connecting pipe (310) and the air-drying plate (311), and the pipette tip is dried and disinfected by the disinfection lamp (312).

Images