A solid-state fermentation liquid strain inoculation device

Abstract

The present application relates to the technical field of solid-state equipment fermentation, and in particular to a solid-state fermentation liquid strain inoculation device, comprising an inoculation shell, a heating ring is arranged inside the inoculation shell, an operation port is opened on the side wall of the inoculation shell, a protective door for closing the operation port is slidably installed on the side wall of the inoculation shell, and when the protective door is closed, it is sealed with the inoculation shell, an observation window is also arranged on the protective door, and a rotating column is rotatably installed through the middle of the inoculation shell; the present invention starts a driving motor and a feeding motor, passes a feeding spoon through the feeding hole to take out the cultivated strain, and then inoculates the taken strain into the inoculation bottle through the feeding hole, and then rotates the placement table to mix the strains in the inoculation bottle, so as to realize the automatic inoculation of solid-state fermentation liquid strains.

Description

Technical Field

[0001] The present application relates to the technical field of solid-state equipment fermentation, and in particular to a solid-state fermentation liquid strain inoculation device. Background Art

[0002] Solid-state fermentation refers to a process that uses insoluble solid substrates to cultivate microorganisms. It includes both deep fermentation in which the solid is suspended in a liquid and the process of cultivating microorganisms on wet solid materials with no (or almost no) free water. The inoculation method of transplanting microorganisms into liquid culture medium is called liquid inoculation method. Liquid inoculation method is often used in microbial physiological and biochemical determination and microbial fermentation experimental research. Liquid inoculation method can observe the different growth characteristics and biochemical characteristics of microorganisms for identification.

[0003] Among them, the solid-state fermentation liquid strain inoculation is usually carried out in an inoculation device, such as a solid-state fermentation liquid strain inoculation device with publication number CN111893030B. The above-mentioned prior art relates to the technical field of solid-state fermentation equipment; a feeding mechanism is provided on the upper side of the inner part of the box body, the upper end of the telescopic tube in the feeding mechanism is connected to the lower end of the discharge pipe, and a fixing mechanism is provided on the lower side of the feeding mechanism. The center of the lower surface of the placement plate in the fixing mechanism is fixedly connected to the rotating rod in the rotating mechanism, and the lower end of the rotating rod is screwed to the lower side wall of the box body through a bearing. The box body is a front open structure, and a door body is provided in the front side of the box body. Through holes are provided on the left and right sides of the middle side of the door body. Rubber gloves are provided in the through holes, and the outer periphery of the top of the rubber gloves is fixedly connected to the inner peripheral wall of the through hole; before the fermenter is cooled to a certain temperature, the fermenter is placed inside the present device, and after the cooling is completed, the microbial strains are added to the fermenter without transfer, and the fermenter is directly shaken to speed up the mixing speed, which is not easy to contact other bacteria, saving time and effort.

[0004] However, the above-mentioned prior art still has some defects when inoculating liquid strains for solid-state fermentation: 1. During the inoculation, the above-mentioned prior art requires the staff to wear rubber gloves in the through hole, remove the sealing plug on the fermentation tank with one hand, hold the feeding tube with the other hand, and place a finger on the control switch to allow the strains in the fermentation tank to be fed into the fermentation tank through the telescopic tube. During this inoculation process, the staff's operation is required throughout the process, and automatic inoculation is not possible, and there is still room for improvement.

[0005] 2. The above-mentioned prior art requires that the microbial strains be placed in a storage box before inoculation. During inoculation, the strains inside the storage box enter the fermentation tank through the telescopic tube. However, the inside of the storage box and the telescopic tube are prone to the growth of miscellaneous bacteria, and the high-temperature steam used for disinfection in the above-mentioned prior art cannot disinfect the discharge box and the inside of the telescopic tube, which may cause miscellaneous bacteria to contaminate the strains that need to be inoculated.

[0006] Based on this, and in accordance with the above-mentioned viewpoints, there is still room for improvement in the existing technology for inoculating liquid strains for solid-state fermentation. Summary of the invention

[0007] In order to solve the above technical problems, the present application provides a solid-state fermentation liquid strain inoculation device, which adopts the following technical solutions:

[0008] A solid-state fermentation liquid strain inoculation device comprises an inoculation shell, a heating ring is arranged inside the inoculation shell, an operation port is opened on the side wall of the inoculation shell, a protective door for closing the operation port is slidably installed on the side wall of the inoculation shell, and when the protective door is closed, it is sealed with the inoculation shell, and an observation window is also arranged on the protective door, a rotating column is rotatably installed through the middle of the inoculation shell, a driving motor is installed on the top of the inoculation shell, and the output shaft of the driving motor is connected to the rotating column, and a transmission device is arranged at the bottom of the inoculation shell.

[0009] Preferably, the transmission device includes a first gear installed at the lower end of the rotating column, a mounting ring is installed concentrically with the rotating column at the lower end of the inoculation shell, a transmission gear ring is arranged on the inner side of the lower end of the mounting ring, a rotating plate is rotatably installed at the upper end of the mounting ring, a plurality of evenly arranged transmission columns are circumferentially penetrated on the rotating plate, and the transmission columns are rotatably connected to the rotating plate, a second gear is arranged at the lower end of the transmission column, and the second gear is meshed with the first gear and the transmission gear ring at the same time.

[0010] Preferably, a clamping device is also provided at the upper end of the transmission column, and the clamping device includes a sliding groove opened in the middle part of the top end of the transmission column, a sliding column is slidably installed inside the sliding groove, a placing table is provided at the upper end of the sliding column, an inoculation bottle is placed on the placing table, the lower end of the sliding column is connected to the bottom end of the sliding groove by a connecting spring, a mounting plate is installed at the upper end of the transmission column, and the mounting plate is located above the rotating plate, two mounting holes are symmetrically opened on the mounting plate, clamping arms are hinged in the two mounting holes, and the upper ends of the two clamping arms are inclined toward the inner side of the mounting plate, the middle part of the clamping arm is hinged to the side wall of the sliding column by a connecting rod, and a clamping plate is provided at the upper end of the clamping arm, and the clamping plate is directly in contact with the inoculation bottle through the clamping pad.

[0011] Preferably, a feeding device is provided on the rotating column, and the feeding device includes a feeding arm installed on the upper end of the side wall of the rotating column, a feeding barrel is installed on the end of the feeding arm facing away from the rotating column, a sliding hole is opened on the side wall of the feeding barrel, a feeding column is provided inside the feeding barrel, a sliding block slidingly matched with the sliding hole is provided on the side wall of the feeding column, a rotating shaft is rotatably installed inside the feeding barrel, and the rotating shaft is connected to the feeding column by a thread, a feeding motor is installed on the top of the feeding barrel, and the output shaft of the feeding motor is connected to the upper end of the rotating shaft.

[0012] Preferably, a picking device is provided at the lower end of the feeding column, and the picking device includes a picking arm symmetrically hinged to the lower end of the feeding column, and a picking spoon is provided at the lower end of the picking arm, and the picking spoons are abutted against each other to form a hemispherical shape together, and a sealing gasket is provided at the contact position between the picking spoons, and the upper ends of the picking arms are connected by a reset spring, and a limiting slide groove is symmetrically provided at the lower end of the feeding column, and the limiting slide groove and the limiting slider on the limiting ring are slidably matched to make the limiting ring slidably connected to the lower end of the feeding column, and the limiting ring is in abutment with the upper end of the picking arm, and a fixing ring is also provided at the lower end of the feeding column, and the fixing ring and the limiting ring are connected by a downward pressure spring.

[0013] Preferably, a discharge device is provided on the side wall of the inoculation shell, and the discharge device includes a discharge port opened on the side wall of the inoculation shell, a discharge rack is installed on the inner wall of the inoculation shell, and the discharge rack is located at the lower end of the discharge port, a discharge frame is slidably installed on the upper end of the discharge rack, a bacterial culture bottle is placed inside the discharge frame, a sealing plate is provided on the end of the discharge frame away from the rotating column, the sealing plate seals the discharge port, and a sliding handle is installed on the outer side wall of the sealing plate.

[0014] Preferably, an adjusting element is provided on the material discharging rack, and the adjusting element includes adjusting plates installed on both sides of the material discharging rack, and the adjusting plates are provided with adjusting sliding holes, and the sliding cooperation between the adjusting sliding holes and the adjusting sliders on the connecting plates enables the connecting plates to be slidably connected to the two adjusting plates together, and the connecting plates are provided with material taking holes, and the side walls of the adjusting plates are provided with adjusting holes evenly distributed up and down, and the side walls of the connecting plates are provided with connecting holes, and connecting pins are provided in the connecting holes, and the connecting pins pass through the connecting holes and the adjusting holes in sequence.

[0015] Preferably, the adjusting element also includes an adjusting sleeve evenly arranged on the upper end of the rotating plate in the circumferential direction, and an adjusting column is slidably installed in the adjusting sleeve. A fixing pin is threadedly connected to the side wall of the adjusting sleeve, and the fixing pin is against the adjusting column at one end facing the inner side of the adjusting sleeve. An adjusting circular plate is commonly installed on the upper end of the adjusting column, and a plurality of feeding holes are opened on the adjusting circular plate, and the positions of the feeding holes correspond one-to-one to the positions of the inoculation bottles.

[0016] In summary, the present application includes at least one of the following beneficial technical effects:

[0017] 1. The present invention starts the driving motor and the feeding motor, passes the feeding spoon through the feeding hole to take out the cultivated strains, and then inoculates the taken strains into the inoculation bottle through the feeding hole, and then rotates the placement table to mix the strains in the inoculation bottle, so as to realize the automatic inoculation of solid-state fermentation liquid strains.

[0018] 2. In the process of inoculation of bacterial strains of the present invention, the bacterial strains only contact the material taking spoon. Therefore, in the process of inoculation of bacterial strains of the present device, the chance of the cultivated bacterial strains being infected by foreign bacteria is greatly reduced. In addition, the sealing arrangement of the protective door and the sealing plate and the discharge port prevents foreign bacteria from entering the inoculation shell during inoculation.

[0019] 3. The present invention places the inoculation bottle on the placing table, and the inoculation bottle uses its own gravity to move the sliding column downward, thereby making the upper ends of the clamping arms approach each other. At this time, the inoculation bottle is clamped and fixed by the clamping plate at the upper end of the clamping arm. When taking out the inoculation bottle, it only needs to be pulled upward to make the clamping arm detach from the clamping of the inoculation bottle. This method of fixing the inoculation bottle is simple and effective, and can be adapted to fix inoculation bottles of different specifications. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of the present invention.

[0021] Figure 2 It is a schematic structural diagram of the transmission device of the present invention.

[0022] Figure 3 It is a schematic structural diagram of the clamping device of the present invention.

[0023] Figure 4 It is a structural schematic diagram of the discharge device of the present invention.

[0024] Figure 5 It is a structural schematic diagram of the feeding device of the present invention.

[0025] Figure 6 It is a structural schematic diagram of the material taking device of the present invention.

[0026] Figure 7 It is a schematic diagram of the structure of the regulating element of the present invention.

[0027] Figure 8 It is a schematic diagram of the structure among the adjusting sleeve, adjusting column, fixing pin, adjusting circular plate and feeding hole of the present invention.

[0028] Explanation of reference numerals: 11, inoculation shell; 12, heating ring; 13, operation port; 14, protective door; 15, observation window; 16, rotating column; 17, driving motor; 2, transmission device; 21, first gear; 22, mounting ring; 23, transmission gear ring; 24, rotating plate; 25, transmission column; 26, second gear; 3, clamping device; 31, sliding groove; 32, sliding column; 33, placing table; 34, inoculation bottle; 35, connecting spring; 36, mounting plate; 37, mounting hole; 38, clamping arm; 39, connecting rod; 310, clamping plate; 311, clamping pad; 4, discharge device; 41, discharge port; 42, discharge rack; 43, discharge frame; 44, bacterial culture bottle; 45, sealing plate; 46. ​​Sliding handle; 5. Feeding device; 51. Feeding arm; 52. Feeding barrel; 53. Sliding hole; 54. Feeding column; 55. Sliding block; 56. Rotating shaft; 57. Feeding motor; 6. Picking device; 61. Picking arm; 62. Picking spoon; 63. Sealing pad; 64. Return spring; 65. Limiting slide groove; 66. Limiting ring; 67. Limiting slider; 68. Fixed ring; 69. Pressing spring; 7. Adjusting element; 71. Adjusting plate; 72. Adjusting slide hole; 73. Connecting plate; 74. Adjusting slider; 75. Picking hole; 76. Adjusting hole; 77. Connecting hole; 78. Connecting pin; 81. Adjusting sleeve; 82. Adjusting column; 83. Fixed pin; 84. Adjusting circular plate; 85. Feeding hole. DETAILED DESCRIPTION

[0029] The following is combined with Figures 1 to 8 This application is described in further detail.

[0030] The embodiment of the present application discloses a solid-state fermentation liquid strain inoculation device, which can automatically perform strain inoculation operations and is not easily contaminated by other bacteria.

[0031] refer to Figure 1 , a solid-state fermentation liquid strain inoculation device, comprising an inoculation shell 11, a heating ring 12 is arranged inside the inoculation shell 11, which is used to maintain the temperature inside the inoculation shell 11 to ensure the inoculation conditions and improve the inoculation effect, an operation port 13 is opened on the side wall of the inoculation shell 11, and a protective door 14 for closing the operation port 13 is also slidably installed on the side wall of the inoculation shell 11, and when the protective door 14 is closed, it is sealed with the inoculation shell 11 to ensure that the inside of the inoculation shell 11 is in a sterile environment, so that it is not easy to be interfered by other bacteria during inoculation, and an observation window 15 is also arranged on the protective door 14 for observing the inoculation situation inside the inoculation shell 11, a rotating column 16 is rotatably installed through the middle part of the inoculation shell 11, a driving motor 17 is installed on the top of the inoculation shell 11, and the output shaft of the driving motor 17 is connected to the rotating column 16, a transmission device 2 is arranged at the bottom of the inoculation shell 11, and the driving motor 17 drives the rotating column 16 to rotate, thereby assisting the inoculation of bacteria through the transmission device 2.

[0032] Before inoculation, after the inside of the inoculation shell 11 is sterilized and disinfected, the heating ring 12 is started to heat the inside of the inoculation shell 11 to a temperature suitable for bacterial inoculation. After the culture medium is placed inside the inoculation shell 11, the drive motor 17 is started. The drive motor 17 drives the rotating column 16 to rotate, thereby driving the transmission device 2 to inoculate the bacteria.

[0033] refer to Figure 2 , is a structural schematic diagram of the transmission device 2 of the present application, the transmission device 2 includes a first gear 21 installed at the lower end of the rotating column 16, the rotating column 16 rotates to rotate the first gear 21, the lower end of the inoculation shell 11 is concentrically installed with a mounting ring 22 and the rotating column 16, the inner side of the lower end of the mounting ring 22 is provided with a transmission gear ring 23, the upper end of the mounting ring 22 is rotatably installed with a rotating plate 24, a plurality of evenly arranged transmission columns 25 are circumferentially penetrated on the rotating plate 24, and the transmission columns 25 are rotatably connected with the rotating plate 24, a second gear 26 is provided at the lower end of the transmission column 25, and the second gear 26 is meshed with the first gear 21 and the transmission gear ring 23 at the same time, the first gear 21 is driven to rotate through the transmission gear ring 23 and the second gear 26 to rotate the rotating plate 24, and the transmission column 25 rotates at the same time.

[0034] The driving motor 17 drives the first gear 21 to rotate through the rotating column 16. The second gear 26 revolves around the first gear 21 while maintaining its own rotation under the meshing of the transmission ring gear 23 and the first gear 21, thereby rotating the rotating plate 24 and the transmission column 25, so that the bacteria in the inoculation bottle 34 can be mixed after the inoculation is completed.

[0035] refer to Figure 3, is a structural schematic diagram of the clamping device 3 of the present application, a clamping device 3 is also provided at the upper end of the transmission column 25, the clamping device 3 includes a sliding groove 31 opened in the middle of the top of the transmission column 25, a sliding column 32 is slidably installed inside the sliding groove 31, a placing table 33 is provided at the upper end of the sliding column 32, an inoculation bottle 34 is placed on the placing table 33, after the inoculation bottle 34 is placed on the placing table 33, the gravity of the inoculation bottle 34 causes the sliding column 32 to move downward, the lower end of the sliding column 32 is connected to the bottom end of the sliding groove 31 by a connecting spring 35, after the inoculation bottle 34 is picked up from the placing table 33, the sliding column 32 slides upward under the action of the connecting spring 35, and a mounting bracket 33 is installed at the upper end of the transmission column 25 The mounting plate 36 is located above the rotating plate 24. Two mounting holes 37 are symmetrically opened on the mounting plate 36. Clamping arms 38 are hinged in the two mounting holes 37, and the upper ends of the two clamping arms 38 are inclined toward the inner side of the mounting plate 36. The middle part of the clamping arm 38 is hinged to the side wall of the sliding column 32 through a connecting rod 39. When the sliding column 32 moves up and down, the upper ends of the clamping arms 38 approach or move away from each other under the action of the connecting rod 39. A clamping plate 310 is provided at the upper end of the clamping arm 38 for fixing the inoculation bottle 34. The clamping plate 310 is in direct contact with the inoculation bottle 34 through the clamping pad 311 to prevent the pressure of the clamping plate 310 from damaging the inoculation bottle 34.

[0036] Before inoculation, after the inoculation bottle 34 is placed on the placement table 33, the inoculation bottle 34 uses its own gravity to move the sliding column 32 downward, compressing the connecting spring 35. When the sliding column 32 moves downward, the clamping arm 38 rotates in the mounting hole 37 under the action of the connecting rod 39, thereby making the upper ends of the clamping arms 38 approach each other. At this time, the inoculation bottle 34 is clamped and fixed by the clamping plate 310 at the upper end of the clamping arm 38.

[0037] After the inoculation is completed, when taking out the inoculation bottle 34, first pick up the inoculation bottle 34, the sliding column 32 moves upward under the action of the connecting spring 35, and the clamping wall rotates in the mounting hole 37 under the action of the connecting rod 39, thereby making the upper ends of the clamping arms 38 move away from each other to loosen the inoculation bottle 34.

[0038] refer to Figure 4, is a structural schematic diagram of the discharge device 4 of the present application, a discharge device 4 for storing bacterial strains is provided on the side wall of the inoculation shell 11, the discharge device 4 includes a discharge port 41 opened on the side wall of the inoculation shell 11, a discharge rack 42 is installed on the inner wall of the inoculation shell 11, and the discharge rack 42 is located at the lower end of the discharge port 41, and a discharge frame 43 is slidably installed on the upper end of the discharge rack 42, a bacterial strain culture bottle 44 is placed inside the discharge frame 43, after culturing the bacterial strains in the bacterial strain culture bottle 44, the bacterial strain culture bottle 44 is placed in the discharge frame 43, and a sealing plate 45 is provided at one end of the discharge frame 43 away from the rotating column 16, the sealing plate 45 seals the discharge port 41 to prevent miscellaneous bacteria outside the inoculation shell 11 from entering the interior of the inoculation shell 11 through the discharge port 41, and a sliding handle 46 is installed on the outer wall of the sealing plate 45 to facilitate sliding the discharge frame 43.

[0039] When discharging, the discharging frame 43 is slid toward the outside of the inoculation shell 11 by sliding the sliding handle 46, and then the bacterial culture bottle 44 is placed in the discharging frame 43, and then the discharging frame 43 slides toward the inside of the inoculation shell 11. At this time, the sealing plate 45 seals the discharging port 41 to prevent foreign bacteria outside the inoculation shell 11 from entering the inside of the inoculation shell 11 through the discharging port 41.

[0040] refer to Figure 5 , is a schematic diagram of the structure of the feeding device 5 of the present application, a feeding device 5 for transferring the strains in the strain culture bottle 44 to the inoculation bottle 34 is arranged on the rotating column 16, the feeding device 5 comprises a feeding arm 51 installed at the upper end of the side wall of the rotating column 16, the rotating column 16 rotates to make the feeding arm 51 swing, a feeding barrel 52 is installed at the end of the feeding arm 51 away from the rotating column 16, a sliding hole 53 is opened on the side wall of the feeding barrel 52, a feeding column 54 is arranged inside the feeding barrel 52, a sliding block 55 slidably matched with the sliding hole 53 is arranged on the side wall of the feeding column 54, and the sliding hole 53 is slidably engaged with the feeding column 54. The cooperation with the sliding block 55 limits the rotation of the feeding column 54 in the feeding barrel 52, so that the feeding column 54 can only slide up and down in the feeding barrel 52. A rotating shaft 56 is rotatably installed inside the feeding barrel 52, and the rotating shaft 56 is connected to the feeding column 54 through a thread. When the rotating shaft 56 rotates, the feeding column 54 slides up and down in the feeding barrel 52 under the cooperation of the threads of the rotating shaft 56 and the feeding column 54. A feeding motor 57 is installed at the top of the feeding barrel 52, and the output shaft of the feeding motor 57 is connected to the upper end of the rotating shaft 56. The feeding motor 57 drives the rotating shaft 56 to rotate.

[0041] When picking up materials, the feeding motor 57 drives the rotating shaft 56 to rotate, and the feeding column 54 cooperates with the thread of the rotating shaft 56. At the same time, the feeding column 54 moves downward under the cooperation between the sliding hole 53 and the sliding block 55. After the material is picked up, the feeding motor 57 drives the rotating shaft 56 to rotate in the opposite direction. The feeding column 54 cooperates with the thread of the rotating shaft 56. At the same time, the feeding column 54 moves upward under the cooperation between the sliding hole 53 and the sliding block 55.

[0042] refer to Figure 6 , is a schematic diagram of the structure of the material taking device 6 of the present application. The lower end of the feeding column 54 is provided with a material taking device 6 for taking out the bacterial strain. The material taking device 6 includes a material taking arm 61 symmetrically hinged to the lower end of the feeding column 54. The lower end of the material taking arm 61 is provided with a material taking spoon 62, and the material taking spoon 62 abuts against each other to form a hemispherical shape, which is convenient for taking out the bacterial strain. The contact parts of the material taking spoon 62 are provided with a sealing gasket 63. When the material taking spoon 62 is closed to each other, the bacterial strain in the material taking spoon 62 is prevented from leaking out. The upper end of the material taking arm 61 is connected by a reset spring 64. When the material taking spoon 62 is put down, the material taking spoon 62 is When the strain is removed, the return spring 64 drives the material picking arm 61 to separate, and a limiting slide groove 65 is symmetrically provided at the lower end of the feeding column 54. The limiting slide groove 65 and the limiting slider 67 on the limiting ring 66 slide together to make the limiting ring 66 slidably connected to the lower end of the feeding column 54, and the limiting ring 66 is in conflict with the upper end of the material picking arm 61, and the limiting ring 66 closes the material picking arm 61. A fixing ring 68 is also provided at the lower end of the feeding column 54, and the fixing ring 68 is connected to the limiting ring 66 by a downward pressure spring 69. The limiting ring 66 keeps the two material picking spoons 62 in a closed state through the downward pressure spring 69.

[0043] When taking materials, the culture bottle 44 is located directly below the taking spoon 62, which is convenient for the taking spoon 62 to take materials, the feeding column 54 moves downward, and the limiting ring 66 moves upward relative to the feeding column 54, compressing the downward pressure spring 69. At the same time, the limiting ring 66 disengages from the limiting taking arm 61, and the taking arm 61 separates under the action of the return spring 64. The bacteria enters the taking spoon 62 from the gap between the two taking spoons 62, and then the limiting ring 66 moves downward under the action of the downward pressure spring 69, so that the two taking spoons 62 are in a closed state again. At this time, the taking spoon 62 takes out the bacteria.

[0044] refer to Figure 7 , is a schematic diagram of the structure of the regulating element 7 of the present application. The material discharging rack 42 is provided with the regulating element 7 for assisting the material taking spoon 62 to take out and put down the strains. The regulating element 7 includes regulating plates 71 installed on both sides of the material discharging rack 42. The regulating plates 71 are provided with regulating sliding holes 72. The sliding cooperation between the regulating sliding holes 72 and the regulating sliders 74 on the connecting plates 73 enables the connecting plates 73 to be slidably connected to the two regulating plates 71 together. The connecting plates 73 slide up and down in the regulating plates 71 to adjust the height of the connecting plates 73. A feeding hole 75 is provided on the plate 73, so that the feeding spoon 62 can pass through the feeding hole 75 to take materials, and the feeding hole 75 blocks the limiting ring 66, so that the limiting ring 66 moves upward. The side wall of the adjustment plate 71 is provided with adjustment holes 76 evenly distributed up and down. One of the adjustment holes 76 is selected to fix the connecting plate 73 at a suitable height. A connecting hole 77 is provided on the side wall of the connecting plate 73, and a connecting pin 78 is provided in the connecting hole 77. The connecting pin 78 passes through the connecting hole 77 and the adjustment hole 76 in sequence to fix the connecting plate 73.

[0045] The connecting pin 78 passes through the connecting hole 77 and the adjusting hole 76 in sequence to fix the connecting plate 73 at a suitable height. When the feeding column 54 moves downward, the material taking spoon 62 passes through the material taking hole 75. The material taking hole 75 blocks the limiting ring 66, causing the limiting ring 66 to move upward, thereby separating the two material taking spoons 62 so that the material taking spoons 62 can take the material. When the feeding column 54 is moved upward, the limiting ring 66 moves upward, and the two material taking spoons 62 merge. At this time, the material taking spoon 62 takes out the strain.

[0046] refer to Figure 8 The adjusting element 7 also includes an adjusting sleeve 81 uniformly arranged on the upper end of the rotating plate 24 in the circumferential direction, and an adjusting column 82 is slidably installed in the adjusting sleeve 81. A fixing pin 83 is threadedly connected to the side wall of the adjusting sleeve 81, and the fixing pin 83 faces the inner end of the adjusting sleeve 81 and abuts against the adjusting column 82. After sliding the adjusting column 82 to adjust the height of the adjusting column 82, the fixing pin 83 is rotated in sequence so that the fixing pin 83 abuts against the adjusting column 82 to fix the adjusting column 82. An adjusting circular plate 84 is commonly installed on the upper end of the adjusting column 82, and a plurality of feeding holes 85 are opened on the adjusting circular plate 84, and the positions of the feeding holes 85 correspond to the positions of the inoculation bottles 34 one by one, so that the feeding spoon 62 feeds through the feeding hole 85, and the feeding hole 85 blocks the limiting ring 66, so that the limiting ring 66 moves upward.

[0047] After sliding the adjustment column 82 according to the size of the inoculation bottle 34 to adjust the height of the adjustment column 82, rotate the fixing pin 83 in sequence so that the fixing pin 83 will abut against the adjustment column 82 to fix the adjustment column 82, thereby adjusting the height of the adjustment circular plate 84. When inoculation is performed after the material is taken, rotate the material taking arm 61 so that the inoculation bottle 34 to be inoculated is located directly below the material taking spoon 62, and then move the feeding column 54 downward, and the material taking spoon 62 passes through the feeding hole 85. The feeding hole 85 blocks the limiting ring 66, causing the limiting ring 66 to move upward, thereby separating the two material taking spoons 62, and the bacteria in the two material taking spoons 62 fall into the inoculation bottle 34 for inoculation.

[0048] The embodiments of this specific implementation method are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, all equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A solid-state fermentation liquid strain inoculation device, comprising an inoculation housing (11), characterized in that: A heating ring (12) is arranged inside the inoculation shell (11), an operation port (13) is opened on the side wall of the inoculation shell (11), a protective door (14) for closing the operation port (13) is slidably mounted on the side wall of the inoculation shell (11), and when the protective door (14) is closed, it is sealed with the inoculation shell (11), and an observation window (15) is also arranged on the protective door (14), a rotating column (16) is rotatably installed through the middle of the inoculation shell (11), a driving motor (17) is installed on the top of the inoculation shell (11), and the output shaft of the driving motor (17) is connected to the rotating column (16), and a transmission device (2) is arranged at the bottom of the inoculation shell (11); The transmission device (2) comprises a first gear (21) mounted on the lower end of the rotating column (16); a mounting ring (22) is mounted concentrically with the rotating column (16) at the lower end of the inoculation shell (11); a transmission gear ring (23) is arranged on the inner side of the lower end of the mounting ring (22); a rotating plate (24) is rotatably mounted on the upper end of the mounting ring (22); a plurality of transmission columns (25) are evenly arranged and circumferentially penetrate the rotating plate (24); and the transmission columns (25) are rotatably connected to the rotating plate (24); a second gear (26) is arranged at the lower end of the transmission column (25); and the second gear (26) is meshed with the first gear (21) and the transmission gear ring (23) at the same time; A clamping device (3) is also provided at the upper end of the transmission column (25), and the clamping device (3) includes a sliding groove (31) opened in the middle of the top end of the transmission column (25), a sliding column (32) is slidably installed inside the sliding groove (31), a placement table (33) is provided at the upper end of the sliding column (32), and an inoculation bottle (34) is placed on the placement table (33), and the lower end of the sliding column (32) is connected to the bottom end of the sliding groove (31) via a connecting spring (35); The clamping device (3) further comprises a mounting plate (36) mounted on the upper end of the transmission column (25), and the mounting plate (36) is located above the rotating plate (24), and two mounting holes (37) are symmetrically opened on the mounting plate (36), and clamping arms (38) are hinged in the two mounting holes (37), and the upper ends of the two clamping arms (38) are inclined toward the inner side of the mounting plate (36), and the middle part of the clamping arm (38) is hinged to the side wall of the sliding column (32) through a connecting rod (39), and a clamping plate (310) is arranged at the upper end of the clamping arm (38), and the clamping plate (310) is directly in contact with the inoculation bottle (34) through a clamping pad (311); A feeding device (5) is provided on the rotating column (16), and the feeding device (5) comprises a feeding arm (51) mounted on the upper end of the side wall of the rotating column (16); a feeding barrel (52) is mounted on the end of the feeding arm (51) away from the rotating column (16); a sliding hole (53) is provided on the side wall of the feeding barrel (52); a feeding column (54) is provided inside the feeding barrel (52); a sliding block (55) slidably matched with the sliding hole (53) is provided on the side wall of the feeding column (54); a rotating shaft (56) is rotatably mounted inside the feeding barrel (52), and the rotating shaft (56) is connected to the feeding column (54) by a thread; a feeding motor (57) is mounted on the top end of the feeding barrel (52), and the output shaft of the feeding motor (57) is connected to the upper end of the rotating shaft (56); A material taking device (6) is provided at the lower end of the feeding column (54), and the material taking device (6) comprises a material taking arm (61) symmetrically hinged to the lower end of the feeding column (54), and a material taking spoon (62) is provided at the lower end of each of the material taking arms (61), and the material taking spoons (62) abut against each other to form a hemispherical shape, and a sealing pad (63) is provided at the contacting portion of the material taking spoons (62), and the upper end of the material taking arm (61) is connected via a return spring (64); The material taking device (6) further comprises a limiting slide groove (65) symmetrically provided at the lower end of the feeding column (54); the limiting slide groove (65) and a limiting slider (67) on the limiting ring (66) are slidably matched so that the limiting ring (66) is slidably connected to the lower end of the feeding column (54), and the limiting ring (66) is in contact with the upper end of the material taking arm (61); a fixing ring (68) is further provided at the lower end of the feeding column (54), and the fixing ring (68) and the limiting ring (66) are connected via a downward pressure spring (69).

2. A solid-state fermentation liquid strain inoculation device according to claim 1, characterized in that: A discharge device (4) is arranged on the side wall of the inoculation shell (11), and the discharge device (4) includes a discharge port (41) opened on the side wall of the inoculation shell (11); a discharge rack (42) is installed on the inner side wall of the inoculation shell (11), and the discharge rack (42) is located at the lower end of the discharge port (41); a discharge frame (43) is slidably installed on the upper end of the discharge rack (42), and a bacterial culture bottle (44) is placed inside the discharge frame (43); a sealing plate (45) is arranged at one end of the discharge frame (43) away from the rotating column (16), and the sealing plate (45) seals the discharge port (41); and a sliding handle (46) is installed on the outer side wall of the sealing plate (45).

3. A solid-state fermentation liquid strain inoculation device according to claim 2, characterized in that: The material discharging rack (42) is provided with an adjusting element (7), and the adjusting element (7) comprises adjusting plates (71) mounted on both sides of the material discharging rack (42). The adjusting plates (71) are provided with adjusting sliding holes (72). The sliding cooperation between the adjusting sliding holes (72) and the adjusting sliding blocks (74) on the connecting plate (73) enables the connecting plate (73) to be slidably connected to the two adjusting plates (71). The connecting plate (73) is provided with a material taking hole (75). The side wall of the adjusting plate (71) is provided with adjusting holes (76) evenly distributed up and down. The side wall of the connecting plate (73) is provided with a connecting hole (77). A connecting pin (78) is provided in the connecting hole (77), and the connecting pin (78) passes through the connecting hole (77) and the adjusting hole (76) in sequence.

4. A solid-state fermentation liquid strain inoculation device according to claim 3, characterized in that: The adjusting element (7) further comprises an adjusting sleeve (81) uniformly arranged on the upper end of the rotating plate (24) in the circumferential direction, and an adjusting column (82) is slidably installed in the adjusting sleeve (81), and a fixing pin (83) is threadedly connected to the side wall of the adjusting sleeve (81), and the fixing pin (83) faces the inner end of the adjusting sleeve (81) and abuts against the adjusting column (82), and an adjusting circular plate (84) is installed on the upper end of the adjusting column (82), and a plurality of feeding holes (85) are opened on the adjusting circular plate (84), and the positions of the feeding holes (85) correspond one-to-one to the positions of the inoculation bottles (34).

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