A Chinese herbal medicine extract for relaxing muscles and tendons and its preparation method

Abstract

The invention relates to the technical field of Chinese herbal medicine extraction, and specifically discloses a Chinese herbal medicine extract for relaxing muscles and activating collaterals and a preparation method thereof. The preparation method of the Chinese herbal medicine extract for relaxing muscles and activating collaterals comprises the following steps: step 1), evenly mixing fangfeng, sanqi, angelica, frankincense, chuanxiong, oriental rhizome, chuanxuduan, atractylodes, notopterygium, cinnamon bark, chuanxiong, ginger, bupleurum, saffron, parsley, rice wine lees water to obtain a mixture; step 2), adding composite bacteria to the mixture, fermenting, sterilizing, filtering, taking the filtrate, and obtaining a Chinese herbal medicine extract for relaxing muscles and activating collaterals; the composite bacteria is a compound of Bacillus megaterium, Clostridium cohnii, and Portuguese coryneformis. The invention has the advantages of making the analgesic effect take effect faster and more lasting.

Description

Technical Field

[0001] The invention relates to the field of Chinese herbal medicine extraction, in particular to a Chinese herbal medicine extract for relaxing muscles and tendons and activating blood circulation, and a preparation method thereof. Background Art

[0002] Osteoarthritis is a common bone disease in the elderly. It is a disease caused in part by damage, loss of cartilage structure and function, and dysregulation of pro-inflammatory and anti-inflammatory pathways. Osteoarthritis cannot be completely cured because cartilage tissue is extremely difficult to restore once it is destroyed. The goal of treatment is to relieve pain, improve joint mobility, strengthen joints, and minimize disease progression.

[0003] Usually, the method of treating osteoarthritis is mainly to apply products with relaxing meridians and activating collaterals on the skin surface, and relieve the discomfort caused by osteoarthritis through the anti-inflammatory, analgesic and other efficacy ingredients in the products.

[0004] Liquid-based products for relaxing the meridians and activating blood circulation are popular among consumers because of their ease of use. However, both oily and water-based products have the defect that the active ingredients are difficult to be absorbed by the skin. After application, the products are difficult to reach the affected area directly and will remain on the skin surface in large quantities, resulting in a slow and short-lasting analgesic effect. This makes it difficult to meet the needs of consumers. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to make the analgesic effect take effect faster and more lasting, the present application provides a Chinese herbal medicine extract for relaxing muscles and tendons and a preparation method thereof.

[0006] In the first aspect, the present application provides a method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, using the following technical solution:

[0007] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, comprising the following steps:

[0008] Step 1), mixing fangfeng, sanqi, angelica, frankincense, chuanxiong, oriental water plantain, chuanxiong, atractylodes, notopterygium, cinnamon bark, cyathula, ginger, bupleurum, saffron, parsley, and rice wine lees water to obtain a mixture;

[0009] Step 2), adding composite bacteria to the mixture, fermenting, sterilizing, filtering, and taking the filtrate to obtain a Chinese herbal medicine extract for relaxing muscles and tendons;

[0010] The composite bacteria is a combination of Bacillus megaterium, Clostridium cohnii and Portuguese coryneformis.

[0011] By adopting the above technical scheme, through the combination of siler, notoginseng, angelica, frankincense, chuanxiong, oriental water plantain, chuanxiong, atractylodes, notopterygium, cinnamon, cyathula, ginger, bupleurum, saffron, paridis yunnanensis, and rice wine lees water, the active ingredients in the Chinese herbal medicine extract for relaxing muscles and tendons can accelerate blood circulation, dredge meridians, treat joint inflammation, and relieve muscle soreness.

[0012] Through the fermentation of the composite bacteria composed of Bacillus megaterium, Clostridium cohnii and Portuguese corysporum, the active ingredients can be more easily absorbed by the skin, and the fermentation products of Bacillus megaterium, Clostridium cohnii and Portuguese corysporum can promote the skin to absorb the active ingredients, so that the Chinese herbal extract for relaxing muscles and activating collaterals can be quickly absorbed after being applied to the skin surface, and the active ingredients can reach the affected area directly to quickly relieve pain. Moreover, since the active ingredients are absorbed to the greatest extent and are not easy to volatilize, the pain relief effect can be maintained for a long time, thereby achieving a long-lasting analgesic effect. There is no need to frequently apply the Chinese herbal extract for relaxing muscles and activating collaterals, thus meeting the needs of consumers well.

[0013] Preferably, the mass ratio of the siler, notoginseng, angelica, frankincense, ligusticum chuanxiong, oriental water plantain, radix polygoni multiflori, atractylodes, notopterygium, cinnamon bark, cyathula capitata, ginger, bupleurum, saffron, parsley, and rice wine lees is 9-11:9-11:9-11:9-11:4-6:8-10:4-6:2-4:5-7:7-9:7-9:4-6:0.8-1.2:0.4-0.6:0.4-0.6:1000.

[0014] By adopting the above technical scheme and specifically selecting the mass ratios of siler, notoginseng, angelica, frankincense, ligusticum chuanxiong, oriental water plantain, radix polygoni multiflori, atractylodes, notopterygium, cinnamon bark, cyathula capitata, ginger, bupleurum, saffron, paridis yunnanensis, and rice wine lees water, the active ingredients can cooperate with each other better and the effect of relieving pain can be better.

[0015] Preferably, in the composite bacteria, the mass ratio of Bacillus megaterium, Clostridium cohnii and Corynespora portugalensis is 0.8-0.9:0.2-0.3:1.3-1.5.

[0016] By adopting the above technical scheme and specifically selecting the mass ratio of Bacillus megaterium, Clostridium cohnii and Portuguese yeast, the fermentation product can better promote the skin's absorption of active ingredients, better achieve the effect of rapid pain relief, and make the pain relief more lasting.

[0017] Preferably, in step 2), the mass ratio of the mixture to the composite bacteria is 1078.6-1103.4:2.3-2.7.

[0018] By adopting the above technical solution and specifically selecting the mass ratio of the mixture and the composite bacteria, the fermentation effect is better and the cost can be well controlled, thus having a higher economic value.

[0019] Preferably, in step 2), fermentation is carried out at 35-38°C.

[0020] By adopting the above technical solution and fermenting at 35-38°C, the activity of each bacteria is higher, the fermentation effect is better, and the product quality is better.

[0021] Preferably, in step 2), the fermentation time is 7-9 days.

[0022] By adopting the above technical solution and controlling the fermentation time to 7-9 days, the fermentation is sufficient, the fermentation product has a better effect in promoting the skin to absorb the active ingredients, and the product quality is better.

[0023] Preferably, the siler, notoginseng, angelica, frankincense, ligusticum chuanxiong, oriental water plantain, radix polygoni multiflori, atractylodes, notopterygium, cinnamon bark, cyathula, ginger, bupleurum, saffron, and paridis yunnanensis are all sun-dried Chinese medicinal materials.

[0024] By adopting the above technical solution, the active ingredients can accelerate blood circulation, dredge meridians, treat joint inflammation, and relieve muscle soreness with better effects.

[0025] In the second aspect, the present application provides a Chinese herbal extract for relaxing muscles and tendons, adopting the following technical scheme: a Chinese herbal extract for relaxing muscles and tendons, prepared by the above-mentioned preparation method of the Chinese herbal extract for relaxing muscles and tendons.

[0026] By adopting the above technical scheme, the prepared Chinese herbal medicine extract for relaxing muscles and tendons can quickly relieve pain, and the pain relief effect can be maintained for a long time without the need for frequent use, thus meeting the needs of consumers well.

[0027] In summary, this application has the following beneficial effects:

[0028] 1. Because the present application uses the combination of siler, notoginseng, angelica, frankincense, chuanxiong, oriental water plantain, chuanxuduan, atractylodes, notopterygium, cinnamon, cyathula, ginger, bupleurum, saffron, parsley, and rice wine lees water, the active ingredients in the Chinese herbal extract for relaxing muscles and tendons can accelerate blood circulation, dredge meridians, treat joint inflammation, and relieve muscle soreness.

[0029] 2. In the present application, it is preferred that the active ingredients can be more easily absorbed by the skin under the fermentation of a composite bacteria composed of Bacillus megaterium, Clostridium cohnii and Portuguese corysporum, and the fermentation products of Bacillus megaterium, Clostridium cohnii and Portuguese corysporum can promote the skin to absorb the active ingredients, so that the Chinese herbal extract for relaxing muscles and activating collaterals can be quickly absorbed after being applied to the skin surface, and the active ingredients can directly reach the affected area to quickly relieve pain. Moreover, since the active ingredients are absorbed to the greatest extent and are not easy to volatilize, the pain relief effect can be maintained for a long time, achieving a long-lasting analgesic effect, and there is no need to frequently apply the Chinese herbal extract for relaxing muscles and activating collaterals, which well meets the needs of consumers.

[0030] 3. In the present application, it is preferred to specifically select the mass ratio of Bacillus megaterium, Clostridium cohnii and Portuguese yeast so that the fermentation product can better promote the skin's absorption of active ingredients, better achieve the effect of rapid pain relief, and make the pain relief more lasting. DETAILED DESCRIPTION

[0031] The present application is further described in detail below with reference to the embodiments.

[0032] Example 1

[0033] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0034] Step 1), put 9g of Saposhnikovia divaricata, 9g of Panax notoginseng, 9g of Angelica sinensis, 9g of frankincense, 4g of Chuanxiong, 8g of Alismatis orientalis, 4g of Dipsacus asper, 2g of Atractylodes lancea, 5g of Notopterygium incisum, 7g of Cinnamomum cassia, 7g of Cyathula officinalis, 4g of Ginger, 0.8g of Bupleurum chinense, 0.4g of Saffron, 0.4g of Paris polyphylla, and 1000g of rice wine lees water into a stirring kettle, rotate at 120r / min, stir for 10min, mix evenly, and obtain a mixture.

[0035] Step 2), add 0.8g of Bacillus megaterium, 0.2g of Clostridium cohnii, and 1.3g of Portuguese Corysporum to the mixture, keep the temperature at 35°C, ferment for 7 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0036] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0037] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0038] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D50759.

[0039] Clostridium coli was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67361.

[0040] Portuguese yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-69758.

[0041] Example 2

[0042] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0043] Step 1), 10g of Saposhnikovia divaricata, 10g of Panax notoginseng, 10g of Angelica sinensis, 10g of frankincense, 5g of Chuanxiong, 9g of Alismatis orientalis, 5g of Dipsacus asper, 3g of Atractylodes macrocephala, 6g of Notopterygium incisum, 8g of Cinnamomum cassia, 8g of Cyathula officinalis, 5g of ginger, 1g of Bupleurum chinense, 0.5g of Saffron, 0.5g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the stirring is carried out for 10min, and the mixture is evenly mixed to obtain a mixture.

[0044] Step 2), add 0.85g of Bacillus megaterium, 0.25g of Clostridium cohnii, and 1.4g of Portuguese Corynosporum to the mixture, keep the temperature at 36°C, ferment for 8 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0045] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0046] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0047] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D50759.

[0048] Clostridium coli was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67361.

[0049] Portuguese yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-69758.

[0050] Example 3

[0051] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0052] Step 1), 11g of Saposhnikovia divaricata, 11g of Panax notoginseng, 11g of Angelica sinensis, 11g of frankincense, 6g of Chuanxiong, 10g of Alismatis orientalis, 6g of Dipsacus asper, 4g of Atractylodes macrocephala, 7g of Notopterygium incisum, 9g of Cinnamomum cassia, 9g of Cyathula officinalis, 6g of Ginger, 1.2g of Bupleurum chinense, 0.6g of Saffron, 0.6g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the mixture is stirred for 10min, and the mixture is evenly mixed to obtain a mixture.

[0053] Step 2), add 0.9g of Bacillus megaterium, 0.3g of Clostridium cohnii, and 1.5g of Portuguese Corynosporum to the mixture, keep the temperature at 38°C, ferment for 9 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0054] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0055] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0056] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D50759.

[0057] Clostridium coli was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67361.

[0058] Portuguese yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-69758.

[0059] Comparative Example 1

[0060] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0061] Step 1), 10g of Saposhnikovia divaricata, 10g of Panax notoginseng, 10g of Angelica sinensis, 10g of frankincense, 5g of Chuanxiong, 9g of Alismatis orientalis, 5g of Dipsacus asper, 3g of Atractylodes macrocephala, 6g of Notopterygium incisum, 8g of Cinnamomum cassia, 8g of Cyathula officinalis, 5g of ginger, 1g of Bupleurum chinense, 0.5g of Saffron, 0.5g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the stirring is carried out for 10min, and the mixture is evenly mixed to obtain a mixture.

[0062] Step 2), add 0.85g Bacillus subtilis, 0.25g Clostridium cohnii, and 1.4g Portuguese Corysporum to the mixture, keep a constant temperature of 36°C, ferment for 8 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0063] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0064] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0065] Bacillus subtilis was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-72808.

[0066] Clostridium coli was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67361.

[0067] Portuguese yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-69758.

[0068] Comparative Example 2

[0069] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0070] Step 1), 10g of Saposhnikovia divaricata, 10g of Panax notoginseng, 10g of Angelica sinensis, 10g of frankincense, 5g of Chuanxiong, 9g of Alismatis orientalis, 5g of Dipsacus asper, 3g of Atractylodes macrocephala, 6g of Notopterygium incisum, 8g of Cinnamomum cassia, 8g of Cyathula officinalis, 5g of ginger, 1g of Bupleurum chinense, 0.5g of Saffron, 0.5g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the stirring is carried out for 10min, and the mixture is evenly mixed to obtain a mixture.

[0071] Step 2), add 0.85g of Bacillus megaterium, 0.25g of Bacillus elongatus and 1.4g of Portuguese Corynosporum to the mixture, keep the temperature at 36°C, ferment for 8 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0072] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0073] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0074] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D50759.

[0075] Lysinibacillus elongatus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-62147.

[0076] Portuguese yeast was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-69758.

[0077] Comparative Example 3

[0078] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0079] Step 1), 10g of Saposhnikovia divaricata, 10g of Panax notoginseng, 10g of Angelica sinensis, 10g of frankincense, 5g of Chuanxiong, 9g of Alismatis orientalis, 5g of Dipsacus asper, 3g of Atractylodes macrocephala, 6g of Notopterygium incisum, 8g of Cinnamomum cassia, 8g of Cyathula officinalis, 5g of ginger, 1g of Bupleurum chinense, 0.5g of Saffron, 0.5g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the stirring is carried out for 10min, and the mixture is evenly mixed to obtain a mixture.

[0080] Step 2), add 0.85g of Bacillus megaterium, 0.25g of Clostridium cohnii, and 1.4g of Candida glabrata to the mixture, keep the temperature at 36°C, ferment for 8 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through a 200-mesh filter cloth, take the filtrate, and obtain a Chinese herbal medicine extract for relaxing muscles and tendons.

[0081] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0082] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0083] Bacillus megaterium was purchased from Shanghai Biotechnology Collection Center, strain number: SHBCC D50759.

[0084] Clostridium coli was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-67361.

[0085] Candida glabrata was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67394.

[0086] Comparative Example 4

[0087] A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that it comprises the following steps:

[0088] Step 1), 10g of Saposhnikovia divaricata, 10g of Panax notoginseng, 10g of Angelica sinensis, 10g of frankincense, 5g of Chuanxiong, 9g of Alismatis orientalis, 5g of Dipsacus asper, 3g of Atractylodes macrocephala, 6g of Notopterygium incisum, 8g of Cinnamomum cassia, 8g of Cyathula officinalis, 5g of ginger, 1g of Bupleurum chinense, 0.5g of Saffron, 0.5g of Paris polyphylla, and 1000g of rice wine lees water are put into a stirring kettle, the speed is 120r / min, the stirring is carried out for 10min, and the mixture is evenly mixed to obtain a mixture.

[0089] Step 2), add 0.85g Bacillus subtilis, 0.25g elongated lysine Bacillus, 1.4g glabrata Candida, keep the temperature at 36°C, ferment for 8 days, start stirring, rotate at 60r / min, inject ozone for sterilization, the ozone flow rate is 100ml / min, continue for 10min, then filter through 200 mesh filter cloth, take the filtrate, and obtain the Chinese herbal medicine extract for relaxing muscles and tendons.

[0090] Saposhnikovia divaricata, Panax notoginseng, Chinese angelica, frankincense, Chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula capitata, ginger, Bupleurum chinense, saffron and Paris polyphylla are all commercially available and are sun-dried Chinese medicinal materials.

[0091] The rice wine lees was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0092] Bacillus subtilis was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-72808.

[0093] Lysinibacillus elongatus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-62147.

[0094] Candida glabrata was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67394.

[0095] Experiment 1

[0096] 100 volunteers were recruited, aged 50-60 years old, all suffering from osteoarthritis and joint pain.

[0097] Volunteers used the Chinese herbal medicine extracts for relaxing muscles and tendons and activating collaterals of various embodiments and comparative examples to conduct efficacy tests.

[0098] Volunteers took 5 ml of Chinese herbal extract for relaxing muscles and tendons and applied it to the painful joints. After the application, the timing was started, and the time until the joints were completely pain-free was recorded, which was recorded as the analgesic time. The timing was started again when the joints were completely pain-free, and the time until the joints felt pain again was recorded, which was recorded as the effective duration of the analgesia.

[0099] 100 volunteers used the same Chinese herbal medicine extract for relaxing muscles and tendons or activating collaterals of the embodiment or comparative example at the same time, and the experimental data were averaged.

[0100] A 48 h interval was required between multiple experiments using different examples or comparative examples.

[0101] The specific experimental data of Experiment 1 are shown in Table 1.

[0102] Table 1

[0103] Pain relief time (min) Effective duration of analgesia (h) Example 1 38.16 24.88 Example 2 36.85 25.35 Example 3 35.97 25.96 Comparative Example 1 78.32 12.85 Comparative Example 2 85.64 13.64 Comparative Example 3 82.49 13.17 Comparative Example 4 91.63 12.19

[0104] According to the data in Table 1, the analgesic time of each embodiment is significantly shorter than that of each comparative example, and the effective analgesic duration of each embodiment is significantly longer than that of each comparative example. It can be seen that after fermentation with Bacillus megaterium, Clostridium cohnii and Portuguese spore yeast, the effective ingredients in siler, Panax notoginseng, Angelica sinensis, frankincense, Ligusticum chuanxiong, Alisma orientalis, Dipsacus asper, Atractylodes macrocephala, Notopterygium wilfordii, cinnamon bark, Cyathula officinalis, ginger, Bupleurum, saffron, Paris polyphylla and rice wine lees water can be well absorbed by the skin, and then quickly reach the affected area, so that the analgesic time is significantly shortened. Moreover, because the active ingredients directly reach the joints, the active ingredients are not easily metabolized out of the body and can stay in the affected area for a long time, achieving a lasting analgesic effect, which significantly improves the effective analgesic duration.

[0105] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that: The following steps are involved: Step 1), mix fangfeng, sanqi, angelica, frankincense, chuanxiong, oriental water, chuanxuduan, atractylodes, notopterygium, cinnamon bark, chuanxiong, ginger, bupleurum, saffron, paridis yunnanensis, and rice wine lees water to obtain a mixture; Step 2), adding composite bacteria to the mixture, fermenting, sterilizing, filtering, and taking the filtrate to obtain a Chinese herbal medicine extract for relaxing muscles and tendons; The composite bacteria is a combination of Bacillus megaterium, Clostridium cohnii and Portuguese coryneformis. The mass ratio of the siler, notoginseng, angelica, frankincense, chuanxiong, oriental water plantain, chuanxiong, atractylodes, notopterygium, cinnamon bark, cyathula, ginger, bupleurum, saffron, parsley, and rice wine lees is 9-11:9-11:9-11:9-11:4-6:8-10:4-6:2-4:5-7:7-9:7-9:4-6:0.8-1.2:0.4-0.6:0.4-0.6:1000; In the composite bacteria, the mass ratio of Bacillus megaterium, Clostridium cohnii and Corynespora portugalensis is 0.8-0.9:0.2-0.3:1.3-1.

5.

2. The method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons according to claim 1, characterized in that: In the step 2), the mass ratio of the mixture to the composite bacteria is 1078.6-1103.4:2.3-2.

7.

3. The method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons according to claim 1, characterized in that: In the step 2), fermentation is carried out at 35-38°C.

4. The method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons according to claim 3, characterized in that: In the step 2), the fermentation time is 7-9 days.

5. The method for preparing a Chinese herbal medicine extract for relaxing muscles and tendons according to claim 1, characterized in that: The siler, notoginseng, angelica, frankincense, ligusticum chuanxiong, oriental water plantain, radix polygoni multiflori, atractylodes, notopterygium, cinnamon bark, cyathula, ginger, bupleurum, saffron and paridis yunnanensis are all sun-dried Chinese medicinal materials.

6. A Chinese herbal medicine extract for relaxing muscles and tendons, characterized in that: The extract is prepared by the method for preparing the Chinese herbal medicine extract for relaxing muscles and tendons and activating collaterals as described in any one of claims 1 to 5.