Rapid detection device based on photoinduced chemiluminescence

Abstract

The embodiment of the present invention discloses a rapid detection device based on photoinduced chemiluminescence, including: a frame module; a bin discharge module, which is arranged on the frame module and corresponds to the discharge port; a mixing module, which is arranged on the frame module; an incubation module, which is arranged on the frame module and arranged on one side of the mixing module; a detection module, which is arranged on the frame module and arranged on one side of the incubation module; and a gripper module, which is arranged on the frame module, and the moving range of the gripper module covers the bin discharge module, the mixing module, the incubation module and the detection module. Since the detection device of this scheme does not involve a liquid circuit structure, there is no need to perform liquid circuit structure maintenance and waste liquid treatment, making the operation and use convenient and simple. This scheme adopts a POCT portable automatic structure design, and the device is small in size and easy to transport and carry. The device operation does not require human intervention, saving time and effort.

Description

Technical Field

[0001] The invention relates to the technical field of immunoassay, and in particular to a rapid detection device based on photoinduced chemiluminescence. Background Art

[0002] Chemiluminescent immunoassay is an analytical method that combines chemiluminescent detection technology with the immune system to detect antigens or antibodies. Photoluminescent immunoassay uses homogeneous system chemiluminescent detection technology. One antibody involved in the immune reaction is coupled to a photosensitive bead; the other antibody is coupled to a luminescent bead, which contains dimethylthiophene derivatives and Eu chelates. It has both the specificity of the immune reaction and the high sensitivity of the chemiluminescent reaction, and has therefore been widely used in clinical practice.

[0003] The chemiluminescent immunoassay instrument is an automated device that controls the immune and biochemical reaction process outside the human body and detects and analyzes the reaction process and results.

[0004] The photochemiluminescence immunoassay analyzer is used in conjunction with the matching microfluidic reagent card. When in use, the various components of the reagents required in the experimental detection process are directly freeze-dried into microspheres and loaded into the corresponding positions of the reagent card. Through the movement of the gripper, barcode recognition, reagent card capping, incubation detection and other operations are realized, the reaction process is automatically controlled, and the reaction results are detected and analyzed.

[0005] However, existing instruments are usually bulky, occupy a lot of installation space, and have limited applicable sites. In addition, the installed liquid path structure requires maintenance and involves waste liquid treatment. The equipment operation is cumbersome, the production and transportation costs are high, and it is time-consuming and labor-intensive. Summary of the invention

[0006] In view of this, the patent of the present invention provides a rapid detection device based on photoinduced chemiluminescence, which is used to solve the problems of large space occupation, complex structure, cumbersome operation and maintenance, time-consuming and labor-intensive, and high cost in the prior art.

[0007] To achieve one, part or all of the above purposes or other purposes, the present invention provides a rapid detection device based on photoinduced chemiluminescence, which comprises:

[0008] Framework module;

[0009] An outbound module, which is arranged on the frame module and corresponds to the material outlet;

[0010] A mixing module, wherein the mixing module is arranged on the frame module;

[0011] An incubation module, wherein the incubation module is disposed on the frame module and arranged on one side of the mixing module;

[0012] a detection module, wherein the detection module is disposed on the frame module and arranged on one side of the incubation module; and

[0013] A gripper module is arranged on the frame module, and the moving range of the gripper module covers the warehouse exit module, the mixing module, the incubation module and the detection module.

[0014] In one embodiment, the detection module includes a detection base, a detection darkroom component and a detection PMT component, the detection darkroom component and the detection PMT component are respectively arranged on the detection base, and the detection PMT component is used to perform detection operations on the test kit in the detection darkroom component.

[0015] In one embodiment, the detection darkroom assembly includes a first photoelectric switch, a receiving light filter, a dichroic mirror, a detection cover, a guide column, a first rack, a rack bracket, a first photoelectric switch baffle, a first motor, a detection door motor seat, a first gear, an excitation light source, an excitation light filter, and an excitation light seat;

[0016] The first photoelectric switch, the receiving light filter and the dichroic mirror are respectively fixed on the detection base, the detection cover is reciprocatingly connected to the detection base through the guide column to open or close the reagent tank of the detection base, the first rack is arranged on the detection cover through the rack bracket, the first photoelectric switch baffle is arranged on the first rack, the first motor is arranged on the detection door motor seat and connected to the first gear, the excitation light source and the excitation light filter are fixed on the excitation light seat, and the excitation light seat is fixed to the detection base.

[0017] In one embodiment, the detection PMT assembly includes a darkroom upper plate, a darkroom shutter, a shutter crank, a rotary solenoid valve and a photon counter. The photon counter and the rotary solenoid valve are respectively fixed to the darkroom upper plate. The darkroom shutter is connected to the rotary solenoid valve via the shutter crank and a cylindrical pin. The photon counter is arranged corresponding to the receiving light filter.

[0018] In one embodiment, the incubation module includes a reaction tray, a heating film, a temperature sensor, an over-temperature protection switch, an incubation tray pillar and thermal insulation cotton. The reaction tray is provided with a plurality of grooves arranged in an array, and the grooves are used to install the test kit. The heating film is arranged on the reaction tray, the temperature sensor is connected to the reaction tray, the over-temperature protection switch is arranged on the reaction tray, the incubation tray pillar is arranged on the bottom surface of the reaction tray, and the thermal insulation cotton is sleeved on the outside of the reaction tray.

[0019] In one embodiment, the mixing module includes a mixing support, a mixing fixed seat, a vibration motor and a fixing buckle, the mixing support is connected to the mixing fixed seat, the mixing fixed seat is used to load the test kit, and the vibration motor is connected to the mixing fixed seat through the fixing buckle.

[0020] In one embodiment, the gripper module includes an X-axis drive module, a Y-axis drive module, a Z-axis drive module and a gripper, the X-axis drive module is connected to the Y-axis drive module, the Y-axis drive module is connected to the Z-axis drive module, and the gripper is arranged on the Z-axis drive module.

[0021] In one embodiment, the gripper includes an upper gripper cover and a lower gripper cover, the lower gripper cover is arranged on the bottom surface of the upper gripper cover, and the bottom surface of the lower gripper cover facing away from the upper gripper cover is provided with pins and columns spaced apart from each other.

[0022] In one embodiment, the bin exit module includes a guide rail fixing seat, a linear guide rail, a slider tray, a material cup tray, a material cup sensor bracket, a second rack, a sensor, a second photoelectric switch, a second photoelectric switch baffle, a motor seat, a second motor, a second gear and a photoelectric bracket;

[0023] The motor seat is arranged on the frame module and connected to the second motor, the second motor is connected to the second gear, the second photoelectric switch is arranged on the frame module through the photoelectric bracket, the guide rail fixing seat is arranged on the frame module, the linear guide rail is connected to the guide rail fixing seat and assembled with the slider tray, the material cup tray is fixed on the slider tray and used for loading the test kit, the material cup sensor bracket is fixed on the slider tray and used for fixing the sensor, the second rack is fixed on the slider tray and meshes with the second gear, and the second photoelectric switch baffle is fixed on the second rack and cooperates with the triggering of the second photoelectric switch.

[0024] In one of the embodiments, the frame module includes a base plate, an X-axis support arm bracket, a Y-axis movable arm bracket, a Y-axis movable arm crossbeam bracket, a waste bin, a rubber foot, and a power socket;

[0025] The X-axis support arm bracket is arranged on the bottom plate and used to support the X-axis drive module, the Y-axis movable arm bracket is arranged on the bottom plate and used to support the Y-axis movable arm crossbeam bracket, the waste bin is arranged on the bottom plate, four rubber feet arranged in a rectangular shape are fixed to the bottom of the bottom plate, and the power socket is arranged on the bottom plate and used to connect an external power supply;

[0026] A circuit installation board is also installed on the bottom plate, and a main control board, a motor drive board and a circuit adapter board are respectively arranged on the circuit installation board.

[0027] Implementing the embodiments of the present invention will have the following beneficial effects:

[0028] In the photoluminescence-based rapid detection device of the present scheme, the warehouse exit module, mixing module, incubation module, detection module and gripper module are integrated and installed in the frame module, and the layout of each functional module is compact, so that the entire detection device is small in size and light in weight, occupies a small installation space, and has a wider range of application scenarios. During the detection operation, each functional module is controlled by a preset program and can automatically cooperate. Specifically, the test kit containing the reagent and sample freeze-dried into microspheres is loaded into the mixing module, and the reagent and sample are fully mixed by the mixing module, and then the gripper module mixes the reagent The box is transferred to the incubation module, which heats the reagent kit and maintains it at the set temperature. Then the gripper module transfers the reagent kit with a suitable temperature from the incubation module to the detection module. The detection module detects the reagent kit and reads the test results. Finally, the gripper module transfers the tested reagent kit to the exit module, which sends the detection device out of the exit module. In this way, the entire automatic detection operation can be completed. Since the liquid circuit structure is not involved, there is no need for liquid circuit structure maintenance and waste liquid treatment, which makes the operation convenient and simple, the detection cost is low, and no human intervention is required, saving time and effort. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] in:

[0031] Figure 1 This is an assembly structure diagram of a rapid detection device based on photoinduced chemiluminescence according to an embodiment;

[0032] Figure 2 for Figure 1 Exploded structure diagram;

[0033] Figure 3 A schematic diagram of the structure of a detection module according to an embodiment;

[0034] Figure 4 for Figure 3 Exploded structural diagram of the darkroom components in the inspection;

[0035] Figure 5 for Figure 3 Exploded structure diagram of the PMT component detected in the test;

[0036] Figure 6 It is an assembly structure diagram of an incubation module of an embodiment;

[0037] Figure 7 for Figure 6 Exploded structure diagram;

[0038] Figure 8 This is an assembly structure diagram of a mixing module according to an embodiment;

[0039] Fig. 9 for Figure 8 Exploded structure diagram;

[0040] Fig.10 This is an assembly structure diagram of a gripper module according to an embodiment;

[0041] Fig.11 for Fig.10 Exploded structure diagram;

[0042] Fig.12 This is an assembly structure diagram of a warehouse exit module according to an embodiment;

[0043] Fig.13 for Fig.12 Exploded structure diagram;

[0044] Fig.14 It is an assembly structure diagram of a frame module of an embodiment;

[0045] Fig.15 for Fig.14 Exploded structure diagram.

[0046] Description of reference numerals:

[0047] 100. Rapid detection device based on photoinduced chemiluminescence; 10. Frame module; 11. Bottom plate; 12. X-axis support arm bracket; 13. Y-axis movable arm bracket; 14. Y-axis movable arm crossbeam bracket; 15. Waste bin; 16. Rubber foot; 17. Power socket; 18. Circuit mounting board; 18a. Main control board; 18b. Motor drive board; 18c. Circuit adapter board; 20. Outlet module; 21. Guide rail fixing seat; 22. Linear guide rail; 23. Slider tray; 2 4. Cup tray; 24a. Cup sensor bracket; 25. Second rack; 26. Sensor; 27. Second photoelectric switch; 28. Second photoelectric switch baffle; 28a. Motor seat; 28b. Second motor; 28c. Second gear; 28d. Photoelectric bracket; 30. Mixing module; 31. Mixing support; 32. Mixing fixed seat; 33. Vibration motor; 34. Fixed buckle; 40. Incubation module; 41. Reaction tray; 42. Heating film; 43. Temperature sensor; 4 4. Over-temperature protection switch; 45. Incubation tray support; 46. Insulation cotton; 50. Detection module; 51. Detection base; 52. Detection darkroom assembly; 521. First photoelectric switch; 522. Receiver filter; 523. Dichroic mirror; 524. Detection cover; 525. Guide column; 526. First rack; 527. First photoelectric switch baffle; 528. First motor; 529. Detection door motor seat; 529a. First gear; 529b. Excitation light source; 529 c. Excitation light filter; 529d. Excitation light seat; 53. Detection PMT assembly; 531. Darkroom upper plate; 532. Darkroom shutter; 533. Shutter crank; 534. Rotary solenoid valve; 535. Photon counter; 60. Gripper module; 61. X-axis drive module; 62. Y-axis drive module; 63. Z-axis drive module; 64. Gripper; 641. Gripper cover upper; 642. Gripper cover lower; 642a. Pin; 642b. Column; 200. Reagent box. DETAILED DESCRIPTION

[0048] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0049] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0050] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0051] like Figure 1 and Figure 2 As shown, a rapid detection device 100 based on photoinduced chemiluminescence according to an embodiment includes: a frame module 10, a bin exit module 20, a mixing module 30, an incubation module 40, a detection module 50 and a gripper module 60. The frame module 10 is formed as a cubic frame structure as a whole, and has an installation cavity inside, which can be used to accommodate the bin exit module 20, the mixing module 30, the incubation module 40, the detection module 50 and the gripper module 60.

[0052] Specifically in this embodiment, Figure 1 From the perspective of , the gripper module 60 is arranged on the rear side of the frame module 10, the incubation module 40 and the warehouse exit module 20 are arranged on the front side of the frame module 10, the detection module 50 and the mixing module 30 are arranged in the middle position of the frame module 10, and are located between the gripper module 60 and the incubation module 40 and the warehouse exit module 20.

[0053] The warehouse outlet module 20 is arranged on the frame module 10 and corresponds to the material outlet; the mixing module 30 is arranged on the frame module 10; the incubation module 40 is arranged on the frame module 10 and arranged on one side of the mixing module 30; the detection module 50 is arranged on the frame module 10 and arranged on one side of the incubation module 40; the gripper module 60 is arranged on the frame module 10, and the moving range of the gripper module 60 covers the warehouse outlet module 20, the mixing module 30, the incubation module 40 and the detection module 50.

[0054] The implementation of the embodiments of the present invention will have the following beneficial effects: in the photoinduced chemiluminescence-based rapid detection device 100 of the present solution, by integrating the warehouse module 20, the mixing module 30, the incubation module 40, the detection module 50 and the gripper module 60 in the frame module 10, the layout of each functional module is compact, so that the entire detection device is small in size and light in weight, occupies a small installation space, and has a wider range of application scenarios. During the detection operation, each functional module is controlled by a preset program and can automatically cooperate. Specifically, the test kit 200 containing the reagent and sample freeze-dried into microspheres is loaded into the mixing module 30, and the reagent and sample are fully mixed by the mixing module 30, and then the gripper module 60 transfers the test kit 200 to the incubation module 40, which heats the test kit 200 and maintains it at a set temperature. Then, the gripper module 60 transfers the test kit 200 with a suitable temperature from the incubation module 40 to the detection module 50. The detection module 50 detects the test kit 200 and reads the test result. Finally, the gripper module 60 transfers the test kit 200 that has completed the test to the exit module 20, which sends the test device out of the exit module 20. In this way, the entire automatic detection operation can be completed. Since the liquid circuit structure is not involved, there is no need to perform liquid circuit structure maintenance and waste liquid treatment, which makes the operation convenient and simple, the detection cost is low, and no human intervention is required, saving time and effort.

[0055] like Figures 3 to 5 As shown, in one embodiment, the detection module 50 includes a detection base 51, a detection darkroom assembly 52 and a detection PMT assembly 53. The detection darkroom assembly 52 and the detection PMT assembly 53 are respectively arranged on the detection base 51, and the detection PMT assembly 53 is used to perform a detection operation on the reagent kit 200 in the detection darkroom assembly 52. ​​The detection base 51 is used to assemble the detection darkroom assembly 52 and the detection PMT assembly 53 on the frame module 10. After the reagent kit 200 to be detected is placed in the detection darkroom assembly 52, the detection PMT assembly 53 can perform a detection operation on the reagent kit 200.

[0056] The detection method is specifically as follows: In one embodiment, the detection darkroom assembly 52 includes a first photoelectric switch 521, a receiving light filter 522, a dichroic mirror 523, a detection cover 524, a guide column 525, a first rack 526, a rack bracket, a first photoelectric switch baffle 527, a first motor 528, a detection door motor seat 529, a first gear 529a, an excitation light source 529b, an excitation light filter 529c, and an excitation light seat 529d.

[0057] The first photoelectric switch 521, the receiving light filter 522 and the dichroic mirror 523 are respectively fixed on the detection base 51, and the detection cover 524 is connected to the detection base 51 for reciprocating movement through the guide column 525 to open or close the reagent tank of the detection base 51. The first rack 526 is set on the detection cover 524 through the rack bracket, the first photoelectric switch baffle 527 is set on the first rack 526, the first motor 528 is set on the detection door motor seat 529 and connected to the first gear 529a, the excitation light source 529b and the excitation light filter 529c are fixed on the excitation light seat 529d, and the excitation light seat 529d is fixed to the detection base 51.

[0058] After the reagent box 200 is placed in the reagent tank, the first motor 528 drives the first gear 529a and the first rack 526 to move, thereby driving the detection cover 524 to close the reagent tank, so that the reagent tank forms a dark room, avoiding the ambient light of the detection room from entering the reagent tank and causing interference, affecting the detection accuracy; through the first photoelectric switch 521 and the first photoelectric switch baffle 527, the blocking trigger can indicate that the detection cover 524 has completely covered the notch of the reagent tank; then, the excitation light source 529b emits excitation light, which is then irradiated to the corresponding detection area of ​​the reagent box 200 through the dichroic mirror 523 to generate corresponding receiving light, and the receiving light is reflected by the dichroic mirror 523 and the excitation light filter 529c, and then reaches the detection PMT component 53 through the receiving light filter 522 for data acquisition.

[0059] like Figure 5 As shown, in addition, in one embodiment, the detection PMT assembly 53 includes a darkroom upper plate 531, a darkroom shutter 532, a shutter crank 533, a rotary solenoid valve 534 and a photon counter 535. The photon counter 535 and the rotary solenoid valve 534 are respectively fixed on the darkroom upper plate 531. The darkroom shutter 532 is connected to the rotary solenoid valve 534 through the shutter crank 533 and a cylindrical pin. The photon counter 535 is arranged corresponding to the receiving light filter 522. During detection, the movement of the rotary solenoid valve 534 can drive the darkroom shutter 532 to open and close, thereby realizing the opening or closing of the detection shutter; when the detection shutter is opened, the photon counter 535 can receive the excitation light transmitted from the receiving light filter 522, thereby realizing the collection of corresponding optical data.

[0060] It should be noted that PMT, the abbreviation of "photomultiplier tube", is a widely used photoelectric conversion device. Its function is to convert and amplify the scintillation light generated by the crystal after being irradiated by gamma rays into electrical signals in proportion, with an amplification factor of up to 10 6 -10 9, so even very weak light can be measured. It is mainly composed of a photocathode, an electron focusing system, a multi-stage dynode and an anode. The entire system is enclosed in a vacuum glass shell. The photocathode is made by spraying some photosensitive materials on the inner side of the tube end window. The function of the photocathode is to convert the photons emitted by the scintillator into low-energy photoelectrons through the photoelectric effect. The dynode is made by vaporizing the photosensitive material on the sickle. There are multiple multiplication stages on the dynode. Each multiplication stage is applied with a voltage that increases in sequence. The electrons emitted by the photocathode are focused and accelerated by the electron focusing system and reach the first dynode. More electrons are generated through the second electron emission, and then multiplied step by step to reach enough electrons. The requirement for the dynode is that the secondary emission coefficient should be large and the multiplied secondary electrons should be effectively sent to the next stage. The anode is usually made of nickel, molybdenum and other materials. Its function is to collect the multiplied electrons and form an electrical pulse signal through the output circuit. This signal is then recorded and analyzed by electronic instruments.

[0061] like Figure 6 and Figure 7 As shown, in one embodiment, the incubation module 40 includes a reaction disk 41, a heating film 42, a temperature sensor 43, an over-temperature protection switch 44, an incubation disk support 45 and thermal insulation cotton 46. The reaction disk 41 is provided with a plurality of grooves arranged in an array, the grooves are used to install the reagent kit 200, the heating film 42 is arranged on the reaction disk 41, the temperature sensor 43 is connected to the reaction disk 41, the over-temperature protection switch 44 is arranged on the reaction disk 41, the incubation disk support 45 is arranged on the bottom surface of the reaction disk 41, and the thermal insulation cotton 46 is sleeved on the outside of the reaction disk 41.

[0062] The reaction disk 41 is a plate structure, which is used to load the reagent kit 200 and heat the reagent kit 200 by heat conduction. Since a plurality of grooves arranged in an array are provided, the reaction disk 41 can accommodate a plurality of reagent kits 200 at the same time, thereby improving the heating efficiency and saving the waiting time for heating to the required temperature; the heating film 42 is used to generate heat to heat the reaction disk 41; the temperature sensor 43 is used to collect the real-time temperature of the reaction disk 41, which is convenient for temperature control; the over-temperature protection switch 44 is used to protect the temperature control reaction of the reaction disk 41 from excessive temperature caused by hardware failure; the incubation disk support 45 is used to fix the incubation module 40 to the instrument bottom plate 11; the heat preservation cotton 46 is used to maintain the temperature of the incubation module 40 and reduce the heat dissipation efficiency.

[0063] like Figure 8 and Fig. 9 As shown, in one embodiment, the mixing module 30 includes a mixing support 31, a mixing fixed seat 32, a vibration motor 33 and a fixing buckle 34, the mixing support 31 is connected to the mixing fixed seat 32, the mixing fixed seat 32 is used to load the reagent kit 200, and the vibration motor 33 is connected to the mixing fixed seat 32 via the fixing buckle 34.

[0064] During the detection, the reagent kit 200 is placed in the mixing holder 32, and then the vibration motor 33 is started. The vibration motor 33 applies vibration to the mixing holder 32, so that the reagents and samples in the reagent kit 200 can be mixed more fully and evenly, thereby helping to improve the accuracy of subsequent detection results.

[0065] like Fig.10 and Fig.11 As shown, in one embodiment, the gripper module 60 includes an X-axis driving module 61, a Y-axis driving module 62, a Z-axis driving module 63 and a gripper 64, the X-axis driving module 61 is connected to the Y-axis driving module 62, the Y-axis driving module 62 is connected to the Z-axis driving module 63, and the gripper 64 is arranged on the Z-axis driving module 63. During the detection operation, the X-axis driving module 61, the Y-axis driving module 62 and the Z-axis driving module 63 respectively output the moving force along the X-axis direction, the Y-axis direction and the Z-axis direction, so that the gripper 64 can be driven to move flexibly in any direction in the installation cavity of the frame module 10, so as to realize the flexible transfer of the reagent kit 200 between different functional modules, and ensure that the detection process is automatically and orderly completed.

[0066] Specifically, the X-axis drive module consists of an X-axis support arm, a linear guide and a stepper motor; the linear guide is fixed on the X-axis support arm to support the forward and backward movement of the Y-axis drive module; the photoelectric switch is fixed on the X-axis support arm and is used for hardware limit of the stepper motor; the synchronous wheel is fixed on the stepper motor; the idler wheel is fixed on the X-axis support arm through the X-axis idler wheel support shaft; the stepper motor drives the X-axis synchronous belt through the synchronous wheel to drive the idler wheel to move.

[0067] The Y-axis drive module consists of a Y-axis support arm, a linear guide, a Y-axis circuit board bracket, an X-axis transmission plate and a stepper motor; the linear guide is fixed on the Y-axis support arm to support the left and right movement of the Z-axis drive module; the photoelectric switch and the X-axis photoelectric baffle are fixed on the Y-axis support arm for hardware limit of the stepper motor; the Y-axis circuit board bracket is fixed on the Y-axis support arm for fixing the corresponding circuit board; the synchronous wheel is fixed on the stepper motor; the idler wheel is fixed on the Y-axis support arm through the Y-axis idler wheel support shaft; the stepper motor drives the Y-axis synchronous belt through the synchronous wheel to drive the idler wheel to move; the idler wheel is fixed on the Y-axis support arm through the Y-axis auxiliary wheel support shaft to assist in supporting the Y-axis drive module; the X-axis transmission plate is fixed on the Y-axis support arm and connected to the linear guide, and connected to the X-axis synchronous belt through the X-axis synchronous belt pressure plate. As the stepper motor of the X-axis drive module moves, it drives the Y-axis drive module to move forward and backward.

[0068] The Z-axis drive module consists of a Z-axis support arm, a linear guide, a Z-axis circuit board bracket, a Z-axis slider fixing plate and a lead screw stepper motor; the linear guide is fixed on the Z-axis support arm to support the gripper 64 to move up and down; the photoelectric switch is fixed on the Z-axis support arm through the Z-axis photoelectric bracket, and the Z-axis photoelectric baffle fixed on the Z-axis support arm is used for the hardware limit of the lead screw stepper motor; the Z-axis circuit board bracket is fixed on the Z-axis support arm to fix the corresponding circuit board; the lead screw stepper motor is fixed on the Z-axis support arm and fixed through the Z-axis slider The board connects the gripper 64 with the linear guide and the lead screw stepper motor to drive the gripper 64 to move up and down; the gripper cover claw 642 is connected to the Z-axis slider fixing plate through the gripper cover claw 641, which is used for the pressing of the reagent box 200 and the box picking and placing actions; the Z-axis support arm is connected to the linear guide, and is connected to the Y-axis synchronous belt through the Y-axis synchronous belt pressure plate. As the stepper motor of the Y-axis drive module moves, the Z-axis drive module is driven to move left and right; the Y-axis photoelectric baffle is fixed on the Y-axis synchronous belt pressure plate, which is used for the hardware limit of the Y-axis drive module.

[0069] like Fig.11 As shown, in an optional embodiment, the gripper 64 includes an upper gripper cover 641 and a lower gripper cover 642. The lower gripper cover 642 is arranged on the bottom surface of the upper gripper cover 641. The bottom surface of the lower gripper cover 642 facing away from the upper gripper cover 641 is provided with pins 642a and columns 642b spaced apart from each other. The upper gripper cover 641 is used to connect the lower gripper cover 642 with the Z-axis drive module assembly, so as to realize the overall rising or falling movement of the gripper 64. The box cover of the reagent box 200 is respectively provided with a first socket and a second socket. During the descent of the gripper 64, the pin 642a and the column 642b are respectively and synchronously inserted into the first socket and the second socket. On the one hand, the pressing action can be realized to press and fix the box cover to the box body, and the reagents and samples are encapsulated in the reagent box 200. On the other hand, with the help of the pin 642a and the column 642b tightly matched with the corresponding socket, the gripper 64 can grasp the reagent box 200 so as to transfer the reagent box 200.

[0070] It can be understood that when the reagent kit 200 is transferred to each functional module, the fastening force between the reagent kit 200 and the side wall of the groove of the reagent kit 200 is greater than the fastening force between the pin 642a and the column 642b and the socket, thereby ensuring that the gripper 64 and the reagent kit 200 are effectively separated.

[0071] like Fig.12 and Fig.13As shown, in one embodiment, the bin exit module 20 includes a guide rail fixing base 21, a linear guide rail 22, a slider tray 23, a material cup tray 24, a material cup sensor bracket 24a, a second rack 25, a sensor 26, a second photoelectric switch 27, a second photoelectric switch baffle 28, a motor base 28a, a second motor 28b, a second gear 28c and a photoelectric bracket 28d.

[0072] The motor seat 28a is arranged on the frame module 10 and connected to the second motor 28b, the second motor 28b is connected to the second gear 28c, the second photoelectric switch 27 is arranged on the frame module 10 through the photoelectric bracket 28d, the guide rail fixing seat 21 is arranged on the frame module 10, the linear guide 22 is connected to the guide rail fixing seat 21, and is assembled with the slider tray 23, the material cup tray 24 is fixed on the slider tray 23 and is used to load the reagent kit 200, the material cup sensor bracket 24a is fixed on the slider tray 23 and is used to fix the sensor 26, the sensor 26 is used to detect whether there is a reagent kit 200 in the material cup tray 24; the second rack 25 is fixed on the slider tray 23 and meshed with the second gear 28c, the second photoelectric switch baffle 28 is fixed on the second rack 25 and cooperates with the second photoelectric switch 27 to trigger.

[0073] After the test is completed, the test kit 200 is grabbed by the gripper 64 and transferred to the material cup tray 24. Then the second motor 28b drives the second gear 28c to drive the second rack 25 to move. During the movement, the linear guide 22 guides and limits the material cup tray 24 to ensure the movement accuracy and stability of the material cup tray 24. When the second photoelectric switch 27 is blocked by the second photoelectric switch 27 and triggered, a feedback signal is given to the second motor 28b to stop the second motor 28b from outputting power, and the material cup tray 24 also moves to the discharge port position accordingly, which is convenient for the test personnel to manually or the material taking robot to automatically take away the test kit 200.

[0074] like Fig.14 and Fig.15 As shown, in one embodiment, the frame module 10 includes a base plate 11, an X-axis support arm bracket 12, a Y-axis movable arm bracket 13, a Y-axis movable arm crossbeam bracket 14, a waste bin 15, a rubber foot 16, and a power socket 17; the X-axis support arm bracket 12 is arranged on the base plate 11 and is used to support the X-axis drive module, the Y-axis movable arm bracket 13 is arranged on the base plate 11 and is used to support the Y-axis movable arm crossbeam bracket 14, and the waste bin 15 is arranged on the base plate 11 for discarding the test kit 200 after the experiment is received; four rubber feet 16 arranged in a rectangular shape are fixed to the bottom of the base plate 11 for supporting the detection device to be stably placed at the use site, and the power socket 17 is arranged on the base plate 11 and is used to connect an external power supply for powering the entire detection device.

[0075] A circuit mounting board 18 is also mounted on the bottom plate 11, and a main control board 18a, a motor drive board 18b and a circuit adapter board 18c are respectively arranged on the circuit mounting board 18. Each functional board is mainly used for executing relevant control programs such as motor drive control, temperature control, and reading control inside the detection device.

[0076] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0077] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A rapid detection device based on photoinduced chemiluminescence, characterized in that: include: Framework module; An outbound module, which is arranged on the frame module and corresponds to the material outlet; A mixing module, wherein the mixing module is arranged on the frame module; An incubation module, wherein the incubation module is disposed on the frame module and arranged on one side of the mixing module; A detection module, wherein the detection module is disposed on the frame module and arranged on one side of the incubation module; A gripper module, wherein the gripper module is disposed on the frame module, and a moving range of the gripper module covers the exit module, the mixing module, the incubation module and the detection module; and The bin exit module includes a guide rail fixing seat, a linear guide rail, a slider tray, a material cup tray, a material cup sensor bracket, a second rack, a sensor, a second photoelectric switch, a second photoelectric switch baffle, a motor seat, a second motor, a second gear and a photoelectric bracket; The motor seat is arranged on the frame module and connected to the second motor, the second motor is connected to the second gear, the second photoelectric switch is arranged on the frame module through the photoelectric bracket, the guide rail fixing seat is arranged on the frame module, the linear guide rail is connected to the guide rail fixing seat and assembled with the slider tray, the material cup tray is fixed on the slider tray and used to load the test kit after the test is completed, the material cup sensor bracket is fixed on the slider tray and used to fix the sensor, the second rack is fixed on the slider tray and meshes with the second gear, and the second photoelectric switch baffle is fixed on the second rack and cooperates with the triggering of the second photoelectric switch.

2. The rapid detection device based on photoinduced chemiluminescence according to claim 1, characterized in that: The detection module includes a detection base, a detection darkroom component and a detection PMT component. The detection darkroom component and the detection PMT component are respectively arranged on the detection base, and the detection PMT component is used to perform detection operations on the reagent kit in the detection darkroom component.

3. The rapid detection device based on photoinduced chemiluminescence according to claim 2, characterized in that: The detection darkroom assembly includes a first photoelectric switch, a receiving light filter, a dichroic mirror, a detection cover, a guide column, a first rack, a rack bracket, a first photoelectric switch baffle, a first motor, a detection door motor seat, a first gear, an excitation light source, an excitation light filter, and an excitation light seat; The first photoelectric switch, the receiving light filter and the dichroic mirror are respectively fixed on the detection base, the detection cover is reciprocatingly connected to the detection base through the guide column to open or close the reagent tank of the detection base, the first rack is arranged on the detection cover through the rack bracket, the first photoelectric switch baffle is arranged on the first rack, the first motor is arranged on the detection door motor seat and connected to the first gear, the excitation light source and the excitation light filter are fixed on the excitation light seat, and the excitation light seat is fixed to the detection base.

4. The rapid detection device based on photoinduced chemiluminescence according to claim 3, characterized in that: The detection PMT component includes a darkroom upper plate, a darkroom shutter, a shutter crank, a rotary solenoid valve and a photon counter. The photon counter and the rotary solenoid valve are respectively fixed to the darkroom upper plate. The darkroom shutter is connected to the rotary solenoid valve through the shutter crank and a cylindrical pin. The photon counter is arranged corresponding to the receiving light filter.

5. The rapid detection device based on photoinduced chemiluminescence according to claim 1, characterized in that: The incubation module includes a reaction tray, a heating film, a temperature sensor, an over-temperature protection switch, an incubation tray support and thermal insulation cotton. The reaction tray is provided with a plurality of grooves arranged in an array, and the grooves are used to install the test kit. The heating film is arranged on the reaction tray, the temperature sensor is connected to the reaction tray, the over-temperature protection switch is arranged on the reaction tray, the incubation tray support is arranged on the bottom surface of the reaction tray, and the thermal insulation cotton is sleeved on the outside of the reaction tray.

6. The rapid detection device based on photoinduced chemiluminescence according to claim 1, characterized in that: The mixing module includes a mixing support, a mixing fixing seat, a vibration motor and a fixing buckle, the mixing support is connected to the mixing fixing seat, the mixing fixing seat is used to load the reagent kit, and the vibration motor is connected to the mixing fixing seat through the fixing buckle.

7. The rapid detection device based on photoinduced chemiluminescence according to claim 1, characterized in that: The gripper module includes an X-axis driving module, a Y-axis driving module, a Z-axis driving module and a gripper, the X-axis driving module is connected to the Y-axis driving module, the Y-axis driving module is connected to the Z-axis driving module, and the gripper is arranged on the Z-axis driving module.

8. The rapid detection device based on photoinduced chemiluminescence according to claim 7, characterized in that: The gripper comprises an upper gripper cover and a lower gripper cover. The lower gripper cover is arranged on the bottom surface of the upper gripper cover. The bottom surface of the lower gripper cover facing away from the upper gripper cover is provided with pins and columns spaced apart from each other.

9. The rapid detection device based on photoinduced chemiluminescence according to claim 1, characterized in that: The frame module includes a bottom plate, an X-axis support arm bracket, a Y-axis movable arm bracket, a Y-axis movable arm crossbeam bracket, a waste bin, a rubber foot, and a power socket; The X-axis support arm bracket is arranged on the base plate and is used to support the X-axis drive module, the Y-axis movable arm bracket is arranged on the base plate and is used to support the Y-axis movable arm crossbeam bracket, the waste bin is arranged on the base plate, and four rubber feet arranged in a rectangular shape are fixed to the bottom of the base plate, and the power socket is arranged on the base plate and is used to connect an external power supply.

10. The rapid detection device based on photoinduced chemiluminescence according to claim 9, characterized in that: A circuit installation board is also installed on the bottom plate, and a main control board, a motor drive board and a circuit adapter board are respectively arranged on the circuit installation board.

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