Preparation process of probiotic powder for promoting digestion

Abstract

The present invention provides a preparation process of a probiotic powder for promoting digestion, the preparation process comprising the following steps: (1) preparing freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, and freeze-dried powder of Bifidobacterium bifidum; (2) mixing the freeze-dried powder, inulin, oligofructose, and lactitol to obtain a mixed powder; (3) adding the mixed powder to water, stirring clockwise to obtain a mixed solution; (4) concentrating the mixed solution into a soft material in a vacuum concentrator at 10-15°C, passing through a 20-40 mesh sieve to obtain wet granules; (5) adding the wet granules to a vacuum freeze dryer for vacuum freeze drying, freeze drying in the vacuum freeze dryer to obtain a dry concentrate; (6) grinding the dry concentrate to obtain a powder raw material to obtain a probiotic powder for promoting digestion. The probiotic powder of the present invention has a good digestion-promoting effect and a high degree of comfort when taken.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotics, and particularly relates to a preparation process of probiotic powder for promoting digestion. Background Art

[0002] Probiotics can help restore the balance of intestinal flora, promote digestive tract function, and enhance immunity. There are some probiotic products designed for the elderly on the market, such as Wei Le Shu, Life-Space Adult Probiotics, Nature's Way, etc. The live bacteria ingredients such as Bifidobacterium lactis and Lactobacillus acidophilus in these products can help improve digestion and absorption function. Although the probiotic powder products for the elderly on the market currently consider the issue of promoting digestion. However, due to the weak intestinal function and poor digestion and absorption ability of the elderly, there is still a problem of incomplete consideration of nutrient absorption and product comfort. Therefore, a probiotic powder that promotes digestion for the elderly is of great significance. Summary of the invention

[0003] The purpose of the invention is to provide a preparation process of probiotic powder for promoting digestion.

[0004] In order to achieve the above object, the present invention provides the following technical solutions:

[0005] A preparation process of a probiotic powder for promoting digestion, the preparation process comprising the following steps:

[0006] (1) The strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, and Bifidobacterium bifidum are respectively expanded and cultured in a conventional sterilized culture medium for 2 to 3 times to obtain probiotic liquids; the probiotic liquids are centrifuged and washed with physiological saline to obtain high-concentration liquids; the high-concentration liquids are protected by a freeze-drying protective agent to protect the activity of the cells to obtain probiotic cells; and the probiotic cells are respectively obtained by pre-freezing and freeze-drying to obtain freeze-dried powders of Lactobacillus acidophilus, freeze-dried powders of Bifidobacterium animalis, freeze-dried powders of Lactobacillus plantarum, freeze-dried powders of Lactobacillus casei, and freeze-dried powders of Bifidobacterium bifidum;

[0007] (2) mixing freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, freeze-dried powder of Bifidobacterium bifidum, inulin, oligofructose and lactitol to obtain a mixed powder;

[0008] (3) Add the mixed powder into water with a mass 2-3 times that of the mixed powder and stir clockwise to obtain a mixed solution;

[0009] (4) Concentrate the mixed solution into a soft material in a vacuum concentrator at 10-15°C, and pass it through a 20-40 mesh sieve to obtain wet granules;

[0010] (5) adding the wet granules into a vacuum freeze dryer for vacuum freeze drying, and freeze drying in the vacuum freeze dryer to obtain a dry concentrate;

[0011] (6) Grinding the dried concentrate to obtain a powdered raw material to prepare a digestion-promoting probiotic powder.

[0012] Most of the probiotic powders currently on the market are selected as main strains of Bifidobacterium lactis and Lactobacillus acidophilus, but in the actual research and development process, it is found that these two strains are slower in improving the intestinal function of the elderly, and the effect of long-term use is not ideal. The present invention attempts to add several bacteria on the basis of these two kinds of bacteria, realizes the lifting of efficacy, but the effect is not ideal. Through a large number of experiments, the present invention selects Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, Bifidobacterium bifidum as composite strains, and the probiotic powder prepared can be good for the elderly to promote digestion. These several bacteria are more suitable for the elderly's intestinal health after compounding, and the interaction between the strains can affect the overall effect of the probiotic product, and the compatibility of these several strain combinations is better, for improving the elderly's intestinal health, and the digestion effect is better.

[0013] Furthermore, the bacterial content of Lactobacillus acidophilus freeze-dried powder is 2×10 11 cfu / g-5.0×10 11 cfu / g; the bacterial content of animal Bifidobacterium freeze-dried powder is 4×10 11 cfu / g-7.0×10 11 cfu / g; the bacterial content in the freeze-dried powder of Lactobacillus plantarum is 0.2×10 11 cfu / g-0.4×10 11 cfu / g; the bacterial content of Lactobacillus casei freeze-dried powder is 0.7×10 11 cfu / g-1.0×10 11 cfu / g; the bacterial content of Bifidobacterium bifidum freeze-dried powder is 0.5×10 11 cfu / g-0.8×10 11 cfu / g.

[0014] The intestinal function of the elderly is generally weak, and discomfort may occur during the process of taking probiotic powder, such as abdominal distension, diarrhea, and even lower abdominal pain. Although this phenomenon does not last long, it will affect the consumer's experience. Adding strains of a specific concentration range to the probiotic thalline of the present invention can make the taking of probiotics more comfortable and the experience better. At the same time, the strains of a specific ratio can also improve the disease resistance of the elderly, and the number of colds is significantly reduced.

[0015] Further, 1-1.4 parts by mass of Lactobacillus acidophilus freeze-dried powder, 0.3-0.6 parts by mass of Bifidobacterium animalis freeze-dried powder, 2.3-2.6 parts by mass of Lactobacillus plantarum freeze-dried powder, 1.7-2.1 parts by mass of Lactobacillus casei freeze-dried powder, 0.7-1.1 parts by mass of Bifidobacterium bifidum freeze-dried powder, 5-9 parts by mass of inulin, 12-17 parts by mass of fructooligosaccharides, and 20-26 parts by mass of lactitol are mixed to obtain a mixed powder.

[0016] Furthermore, the total weight parts of freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, and freeze-dried powder of Bifidobacterium bifidum is ≥ 6.8 weight parts.

[0017] Furthermore, the ratio between the total mass of Lactobacillus acidophilus freeze-dried powder, Bifidobacterium animalis freeze-dried powder, Lactobacillus plantarum freeze-dried powder, Lactobacillus casei freeze-dried powder, and Bifidobacterium bifidum freeze-dried powder and the total mass of inulin and oligofructose lactitol is (6.8-7.1): (43-47).

[0018] The probiotics of the present invention and the specific prebiotics are carefully designed to ensure the activity of the probiotics and provide sufficient prebiotics to support their growth. And when the total mass of Lactobacillus acidophilus freeze-dried powder, animal Bifidobacterium freeze-dried powder, plant lactobacillus freeze-dried powder, casei freeze-dried powder, and Bifidobacterium bifidum freeze-dried powder is ≥6.8 mass parts, and the total mass of Lactobacillus acidophilus freeze-dried powder, animal Bifidobacterium freeze-dried powder, plant lactobacillus freeze-dried powder, casei freeze-dried powder, and Bifidobacterium bifidum freeze-dried powder is (6.8-7.1): (43-47) to the total mass of inulin and oligofructose lactitol, the probiotic powder of the present invention has a better effect. Analysis shows that the prebiotics of the present invention, such as inulin, oligofructose, etc., can be used as a food source for probiotics, play a synergistic role with probiotics, and promote the growth of beneficial bacteria in the intestine. The proliferation of these beneficial bacteria can change the intestinal microenvironment.

[0019] Furthermore, in step (3), the stirring speed is 80-90 r / min, and the stirring time is 40-45 min.

[0020] Furthermore, in step (5), the freeze-drying temperature in the vacuum freeze dryer is -20°C to -30°C, the vacuum degree is 5-10Pa, and the vacuum freezing time is 12-15h.

[0021] Furthermore, in step (6), a powder raw material having a particle size of less than 100-200 mesh is obtained.

[0022] Furthermore, 1.2 parts by mass of Lactobacillus acidophilus freeze-dried powder, 0.5 parts by mass of Bifidobacterium animalis freeze-dried powder, 2.5 parts by mass of Lactobacillus plantarum freeze-dried powder, 1.9 parts by mass of Lactobacillus casei freeze-dried powder, 0.9 parts by mass of Bifidobacterium bifidum freeze-dried powder, 7 parts by mass of inulin, 15 parts by mass of oligofructose, and 23 parts by mass of lactitol are mixed to obtain a mixed powder.

[0023] Furthermore, in step (1), the product is pre-frozen at 80°C for 24-28 hours, and freeze-dried at 80°C and 0.05-0.1 mBar.

[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0025] 1. The present invention selects Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, and Bifidobacterium bifidum as composite bacteria, and the prepared probiotic powder can promote digestion for the elderly and improve constipation.

[0026] 2. Adding bacteria of a specific concentration range to the probiotics of the present invention can make the taking of probiotics more comfortable and provide a better experience. At the same time, the bacteria of a specific ratio can also improve the disease resistance of the elderly, and the number of colds can be significantly reduced.

[0027] 3. The ratio of the probiotics and the specific prebiotics of the present invention is carefully designed, which not only ensures the activity of the probiotics but also provides sufficient prebiotics to support their growth, and at the same time has a significant effect in protecting the intestines and maintaining the survival of beneficial intestinal bacteria. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The raw materials used in the present invention are all commercially available products, specifically purchased from the following manufacturers:

[0030] Lactobacillus acidophilus, accession number: CCTCC AB 2010208. Purchased from China Center for Type Culture Collection.

[0031] Bifidobacterium animalis, accession number: SHBCC D24407AS 1.1852, was purchased from China Center for Type Culture Collection.

[0032] Lactobacillus plantarum, accession number: CCTCC AB 2010210. It was purchased from China Center for Type Culture Collection.

[0033] Lactobacillus casei, accession number: SHBCC D24737, was purchased from Shanghai Microbiological Collection Center.

[0034] Bifidobacterium bifidum, accession number: SHBCC D24408 ATCC 35914, was purchased from Shanghai Microbiological Collection Center.

[0035] Bifidobacterium lactis, accession number: SHBCC D24675, was purchased from Shanghai Microbiological Collection Center.

[0036] Lactobacillus bulgaricus, deposit number: CCTCC CB 20082295; purchased from China Center for Type Culture Collection.

[0037] Inulin, purchased from Lanzhou Waterless Biotechnology Co., Ltd., Jerusalem artichoke extract.

[0038] Fructooligosaccharide was purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0039] Lactitol, Shandong Aicai Biotechnology Co., Ltd.

[0040] The freeze-drying protective agent includes, by mass concentration, 120 g / L of trehalose, 40 g / L of maltodextrin and 32 g / L of glycerol, and the balance is deionized water.

[0041] Example 1

[0042] This embodiment provides a preparation process of a probiotic powder for promoting digestion, and the preparation process comprises the following steps:

[0043] (1) The strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, and Bifidobacterium bifidum were respectively expanded and cultured in a conventional sterilized culture medium for three times to obtain probiotic liquids; the probiotic liquids were centrifuged and washed with physiological saline to obtain high-concentration liquids; the high-concentration liquids were protected by freeze-drying protective agents to obtain probiotic cells; the cells were pre-frozen and pre-frozen at 80°C for 26 hours, and freeze-dried at 80°C and 0.07 mBar to obtain freeze-dried powders of Lactobacillus acidophilus, freeze-dried powders of Bifidobacterium animalis, freeze-dried powders of Lactobacillus plantarum, freeze-dried powders of Lactobacillus casei, and freeze-dried powders of Bifidobacterium bifidum; the content of bacteria in the freeze-dried powder of Lactobacillus acidophilus was 4.0×10 11 cfu / g; the bacterial content of animal Bifidobacterium freeze-dried powder is 6×10 11 cfu / g; the bacterial content in the freeze-dried powder of Lactobacillus plantarum is 0.3×10 11 cfu / g; the bacterial content of Lactobacillus casei freeze-dried powder is 0.9×10 11 cfu / g; the bacterial content of Bifidobacterium bifidum freeze-dried powder is 0.7×10 11cfu / g;

[0044] (2) 1.2 parts by weight of freeze-dried Lactobacillus acidophilus powder, 0.5 parts by weight of freeze-dried Bifidobacterium animalis powder, 2.5 parts by weight of freeze-dried Lactobacillus plantarum powder, 1.9 parts by weight of freeze-dried Lactobacillus casei powder, 0.9 parts by weight of freeze-dried Bifidobacterium bifidum powder, 7 parts by weight of inulin, 15 parts by weight of oligofructose, and 23 parts by weight of lactitol were mixed to obtain a mixed powder;

[0045] (3) Add the mixed powder into water with a mass twice that of the mixed powder, stir clockwise at a speed of 85 r / min for 42 min to obtain a mixed solution;

[0046] (4) Concentrate the mixed solution into a soft material in a vacuum concentrator at 12°C, and pass it through a 30-mesh sieve to obtain wet granules;

[0047] (5) adding the wet granules to a vacuum freeze dryer for vacuum freeze drying, wherein the freeze drying temperature in the vacuum freeze dryer is -25°C, the vacuum degree is 8 Pa, the vacuum freezing time is 13 h, and freeze drying in the vacuum freeze dryer to obtain a dry concentrate;

[0048] (6) Grinding the dried concentrate to obtain a powder material with a particle size of less than 100 meshes, thereby preparing a probiotic powder that promotes digestion.

[0049] Example 2

[0050] This embodiment provides a preparation process of a probiotic powder for promoting digestion, and the preparation process comprises the following steps:

[0051] (1) The strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, and Bifidobacterium bifidum were expanded and cultured twice in a conventional sterilized culture medium to obtain probiotic liquids; the probiotic liquids were centrifuged and washed with physiological saline to obtain high-concentration liquids; the high-concentration liquids were protected by freeze-drying protective agents to obtain probiotic cells; the content of Lactobacillus acidophilus freeze-dried powder was 5.0×10 11 cfu / g; the bacterial content of animal Bifidobacterium freeze-dried powder is 4×10 11 cfu / g; the bacterial content in the freeze-dried powder of Lactobacillus plantarum is 0.4×10 11 cfu / g; the bacterial content of Lactobacillus casei freeze-dried powder is 0.7×10 11 cfu / g; the bacterial content of Bifidobacterium bifidum freeze-dried powder is 0.8×10 11 cfu / g;

[0052] After pre-freezing and pre-freezing at 80°C for 24 hours, and freeze-drying at 80°C and 0.1 mBar, freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, and freeze-dried powder of Bifidobacterium bifidum were prepared respectively;

[0053] (2) 1.2 parts by weight of Lactobacillus acidophilus freeze-dried powder, 0.6 parts by weight of Bifidobacterium animalis freeze-dried powder, 2.3 parts by weight of Lactobacillus plantarum freeze-dried powder, 2.1 parts by weight of Lactobacillus casei freeze-dried powder, 0.7 parts by weight of Bifidobacterium bifidum freeze-dried powder, 6 parts by weight of inulin, 17 parts by weight of oligofructose, and 20 parts by weight of lactitol were mixed to obtain a mixed powder;

[0054] (3) Add the mixed powder into water with a mass three times that of the mixed powder, stir clockwise at a speed of 80 r / min for 45 min to obtain a mixed solution;

[0055] (4) Concentrate the mixed solution into a soft material in a vacuum concentrator at 15°C, and pass it through a 20-mesh sieve to obtain wet granules;

[0056] (5) adding the wet granules to a vacuum freeze dryer for vacuum freeze drying, wherein the freeze drying temperature in the vacuum freeze dryer is -20°C, the vacuum degree is 10 Pa, the vacuum freezing time is 12 h, and freeze drying in the vacuum freeze dryer to obtain a dry concentrate;

[0057] (6) Grinding the dried concentrate to obtain a powder raw material with a particle size of less than 200 meshes, thereby preparing a probiotic powder that promotes digestion.

[0058] Comparative Example 1

[0059] The difference between this comparative example and Example 1 is that Bifidobacterium bifidum is replaced by Bifidobacterium lactis.

[0060] Comparative Example 2

[0061] The difference between this comparative example and Example 1 is that Lactobacillus plantarum is replaced by Lactobacillus bulgaricus.

[0062] Comparative Example 3

[0063] The difference between this comparative example and Example 1 is that the bacterial content in the freeze-dried powder of Lactobacillus acidophilus is 0.3×10 11 cfu / g; the bacterial content of animal Bifidobacterium freeze-dried powder is 3×10 11 cfu / g; the bacterial content of Lactobacillus plantarum freeze-dried powder is 4.0×10 11 cfu / g; the bacterial content of Lactobacillus casei freeze-dried powder is 1.9×10 11 cfu / g; the bacterial content of Bifidobacterium bifidum freeze-dried powder is 2.7×10 11cfu / g.

[0064] Comparative Example 4

[0065] The difference between this comparative example and Example 1 is that 2.2 parts by mass of Lactobacillus acidophilus freeze-dried powder, 1.5 parts by mass of Bifidobacterium animalis freeze-dried powder, 2.5 parts by mass of Lactobacillus plantarum freeze-dried powder, 2.9 parts by mass of Lactobacillus casei freeze-dried powder, 1.9 parts by mass of Bifidobacterium bifidum freeze-dried powder, 6 parts by mass of inulin, 14 parts by mass of oligofructose, and 21 parts by mass of lactitol are mixed to obtain a mixed powder.

[0066] Comparative Example 5

[0067] The difference between this comparative example and Example 1 is that 1.2 parts by mass of Lactobacillus acidophilus freeze-dried powder, 0.5 parts by mass of Bifidobacterium animalis freeze-dried powder, 2.5 parts by mass of Lactobacillus plantarum freeze-dried powder, 1.9 parts by mass of Lactobacillus casei freeze-dried powder, 0.9 parts by mass of Bifidobacterium bifidum freeze-dried powder, 7 parts by mass of inulin, 15 parts by mass of oligofructose, and 23 parts by mass of lactitol are mixed to obtain a mixed powder.

[0068] Comparative Example 6

[0069] The difference between this comparative example and Example 1 is that 1.0 mass parts of Lactobacillus acidophilus freeze-dried powder, 0.5 mass parts of Bifidobacterium animalis freeze-dried powder, 2.3 mass parts of Lactobacillus plantarum freeze-dried powder, 1.9 mass parts of Lactobacillus casei freeze-dried powder, 0.9 mass parts of Bifidobacterium bifidum freeze-dried powder, 7.2 mass parts of inulin, 15.2 mass parts of oligofructose, and 23 mass parts of lactitol are mixed to obtain a mixed powder.

[0070] Performance Testing

[0071] 450 volunteers aged between 60 and 80 were recruited and randomly divided into 9 groups, with 50 people in each group. The physical conditions of the volunteers were recorded in detail before the experiment. All volunteers had no history of intestinal diseases at present or in the past, and did not use drugs that might affect digestive function. However, all volunteers had varying degrees of constipation and indigestion symptoms.

[0072] Each group of volunteers was provided with the probiotic powder of Examples 1-2 and Comparative Examples 1-6 and a control group (commercially available product: Beijing Tong Ren Tang probiotic powder), 1.5 g of probiotic powder was added to 50 mL of warm water for drinking twice a day, and the control group took 2 bags per day. After 3 months, the number of constipation after 3 months, the number of colds within 3 months and comfort were counted, and the results are shown in Table 1.

[0073] Table 1 Performance test results

[0074]

[0075] It can be seen from the above performance test results that the probiotic powders of Examples 1-2 have good digestion-promoting effects, especially the comprehensive performance of Example 1 is the most outstanding, which is mainly due to the synergistic compounding of the components.

[0076] The comparative examples, however, were significantly inferior to the embodiments in the corresponding performance tests because they did not adopt the necessary technical solutions. In comparative example 1, the species of bacteria were changed, and it can be seen that the digestion-promoting effect and comfort level decreased. In comparative example 2, the bacterial concentration compounding scheme was not used, and the results showed that the comfort level of taking was decreased. The addition amounts of probiotics and prebiotics in comparative examples 4-5 were different, and the effects on inhibiting constipation and reducing colds were different. The above experimental results further demonstrate the importance of the technical solutions defined in the present invention for its technical effects.

[0077] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a probiotic powder for promoting digestion, characterized in that: The following steps are involved: (1) The strains of Lactobacillus acidophilus, Bifidobacterium animalis, Lactobacillus plantarum, Lactobacillus casei, and Bifidobacterium bifidum are respectively expanded and cultured in a conventional sterilized culture medium for 2 to 3 times to obtain probiotic liquids; the probiotic liquids are centrifuged and washed with physiological saline to obtain high-concentration liquids; the high-concentration liquids are protected by a freeze-drying protective agent to protect the activity of the cells to obtain probiotic cells; and the probiotic cells are respectively obtained by pre-freezing and freeze-drying to obtain freeze-dried powders of Lactobacillus acidophilus, freeze-dried powders of Bifidobacterium animalis, freeze-dried powders of Lactobacillus plantarum, freeze-dried powders of Lactobacillus casei, and freeze-dried powders of Bifidobacterium bifidum; (2) mixing freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei, freeze-dried powder of Bifidobacterium bifidum, inulin, oligofructose and lactitol to obtain a mixed powder; (3) Add the mixed powder into water and stir clockwise to obtain a mixed solution; (4) Concentrate the mixed solution into a soft material in a vacuum concentrator at 10-15°C, and pass it through a 20-40 mesh sieve to obtain wet granules; (5) adding the wet granules into a vacuum freeze dryer for vacuum freeze drying, and freeze drying in the vacuum freeze dryer to obtain a dry concentrate; (6) grinding the dried concentrate to obtain a powdered raw material to prepare a digestion-promoting probiotic powder; Among them, the bacterial content of Lactobacillus acidophilus freeze-dried powder is 4.0×10 11 cfu / g-5.0×10 11 cfu / g; the bacterial content of animal Bifidobacterium freeze-dried powder is 4.0×10 11 cfu / g-6.0×10 11 cfu / g; the bacterial content in the freeze-dried powder of Lactobacillus plantarum is 0.3×10 11 cfu / g-0.4×10 11 cfu / g; the bacterial content of Lactobacillus casei freeze-dried powder is 0.7×10 11 cfu / g-0.9×10 11 cfu / g; the bacterial content of Bifidobacterium bifidum freeze-dried powder is 0.7×10 11 cfu / g-0.8×10 11 cfu / g; In step (2), 1.2 parts by weight of Lactobacillus acidophilus freeze-dried powder, 0.5-0.6 parts by weight of Bifidobacterium animalis freeze-dried powder, 2.3-2.5 parts by weight of Lactobacillus plantarum freeze-dried powder, 1.9-2.1 parts by weight of Lactobacillus casei freeze-dried powder, 0.7-0.9 parts by weight of Bifidobacterium bifidum freeze-dried powder, 6-7 parts by weight of inulin, 15-17 parts by weight of oligofructose, and 20-23 parts by weight of lactitol are mixed to obtain a mixed powder; The Lactobacillus acidophilus has a preservation number of CCTCC AB 2010208; the Bifidobacterium animalis has a preservation number of SHBCCD24407AS 1.1852; the Lactobacillus plantarum has a preservation number of CCTCC AB 2010210; the Lactobacillus casei has a preservation number of SHBCC D24737; and the Bifidobacterium bifidum has a preservation number of SHBCC D24408ATCC 35914.

2. The preparation method according to claim 1, characterized in that: The total weight parts of freeze-dried powder of Lactobacillus acidophilus, freeze-dried powder of Bifidobacterium animalis, freeze-dried powder of Lactobacillus plantarum, freeze-dried powder of Lactobacillus casei and freeze-dried powder of Bifidobacterium bifidum are ≥ 6.8 weight parts.

3. The preparation method according to claim 1, characterized in that: The ratio between the total mass of Lactobacillus acidophilus freeze-dried powder, Bifidobacterium animalis freeze-dried powder, Lactobacillus plantarum freeze-dried powder, Lactobacillus casei freeze-dried powder, and Bifidobacterium bifidum freeze-dried powder to the total mass of inulin and oligofructose lactitol (6.8-7.1): (43-47).

4. The preparation method according to claim 1, characterized in that: The stirring speed in step (3) is 80-90 r / min, and the stirring time is 40-45 min.

5. The preparation method according to claim 1, characterized in that: In the step (5), the freeze-drying temperature in the vacuum freeze dryer is -20°C to -30°C, the vacuum degree is 5-10Pa, and the vacuum freezing time is 12-15h.

6. The preparation method according to claim 1, characterized in that: In the step (6), a powder raw material with a particle size of less than 100-200 mesh is obtained.

7. The preparation method according to claim 1, characterized in that: 1.2 parts by mass of Lactobacillus acidophilus freeze-dried powder, 0.5 parts by mass of Bifidobacterium animalis freeze-dried powder, 2.5 parts by mass of Lactobacillus plantarum freeze-dried powder, 1.9 parts by mass of Lactobacillus casei freeze-dried powder, 0.9 parts by mass of Bifidobacterium bifidum freeze-dried powder, 7 parts by mass of inulin, 15 parts by mass of fructooligosaccharides, and 23 parts by mass of lactitol are mixed to obtain a mixed powder.

8. The preparation method according to claim 1, characterized in that: In the step (1), the product is pre-frozen at 80° C. for 24-28 hours and freeze-dried at 80° C. and 0.05-0.1 mBar.

9. A digestion-promoting probiotic powder prepared by the preparation method according to any one of claims 1 to 8.

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