Tooth-cleaning collagen chew for dogs and preparation method of tooth-cleaning collagen chew

Abstract

The invention relates to a tooth cleaning collagen chew for dogs and a preparation method of the tooth cleaning collagen chew, and belongs to the technical field of pet tooth cleaning. The tooth cleaning collagen chew for dogs is prepared from the following components in parts by weight: 38 to 42 parts of cow leather, 38 to 42 parts of gelatin, 14 to 16 parts of rice, 6 to 8 parts of glycerol, 0.4 to 0.6 part of ascophyllum nodosum, 0.1 to 0.5 part of citric acid, 0.2 to 0.9 part of sodium hexametaphosphate and 0.005 to 0.05 part of tea polyphenol. By reasonably selecting and matching the raw materials and improving the production process, the tooth cleaning agent has an excellent tooth cleaning effect, can effectively remove and control dental plaque and dental calculus, is moderate in hardness and high in biting resistance, is easy to digest, and has important market economic value.

Description

Technical Field

[0001] The present application relates to a tooth-cleaning collagen chew for dogs and a preparation method thereof, belonging to the technical field of pet tooth cleaning. Background Art

[0002] With the improvement of national production level, the number of pet owners has increased rapidly, and the pet industry has ushered in rapid development. The role of dogs and cats in people's lives has also changed from working animals to companion animals, providing emotional value to people. With the change of the functional status of pets in the family, pet owners are also more concerned about the health of their pets. Among them, oral problems are common clinical problems for dogs and cats, and many oral problems are closely related to the accumulation of dental plaque and tartar.

[0003] Dental plaque is a biofilm attached to the surface of teeth and composed of various microorganisms and their products, saliva and food residues, while dental calculus is mineralized dental plaque. In addition, oral odor affects the interaction between people and pets. Microbial fermentation in dental plaque is the main cause of oral halitosis, and dental plaque is also the main cause of periodontal disease. Therefore, to maintain the oral health of pets, it is necessary to control the production of dental plaque and dental calculus, and brushing teeth is the best way to remove dental plaque and prevent oral diseases, but it is difficult for pet owners to insist on brushing their pets' teeth every day to keep their mouths clean.

[0004] For dogs and cats who cannot accept or brush their teeth, using tooth-cleaning snacks is a good choice. Currently, the tooth-cleaning snacks for dogs on the market mostly use mechanical friction to remove and control dental plaque and tartar, such as cowhide chew sticks and chewing gums. However, the above traditional cowhide chew sticks and chewing gums are dry and hard to digest, and pet dogs are prone to vomiting, indigestion and other symptoms after eating.

[0005] Chinese patent 202310741444.X discloses a pet tooth-cleaning chewing gum and a preparation method thereof, in which tooth-cleaning tablets and soft puffed tablets are combined to make a soft tooth-cleaning product, and a gum base is added to the soft puffed tablets to give it a chewing taste rather than being bitten off immediately. In combination with the addition of tooth-cleaning ingredients such as green tea and mint extract, the product has obvious tooth-cleaning ability. However, the hardness of the product is too soft, resulting in poor bite resistance, and dogs prefer to chew slightly harder objects. In addition, the hardness is too soft and there is a possibility of sticking to the teeth. In addition, its tooth-cleaning ability needs to be further improved.

[0006] Chinese Patent No. 201711131193.4 discloses an injection-molded tooth-cleaning chew food for pets and its preparation method. The sandwich layer is in the shape of ribs or bones, and is rolled or wrapped with a meat layer on the outside. The unique appearance attracts the pet's attention and increases the fun. The product has good toughness and chewability, strong bite resistance, and is suitable for pets to grind their teeth. The product disclosed in this patent is still a traditional tooth-cleaning product, mainly as a pet food, with the effect of mechanical friction to clean teeth. Although this type of product is more edible, the tooth-cleaning effect is poor.

[0007] Therefore, it is very necessary to develop a dog tooth cleaning product that has good antibacterial effect, moderate hardness, strong bite resistance, and is easy to digest and absorb. Summary of the invention

[0008] In order to solve the above problems, a dog tooth-cleaning collagen chew and a preparation method thereof are provided. In the present application, by reasonably selecting raw materials and improving the production process, the chew not only has excellent tooth-cleaning effect, can effectively remove and control dental plaque and tartar, but also has moderate hardness, strong bite resistance, and is easy to digest.

[0009] According to one aspect of the present application, a tooth-cleaning collagen chew for dogs is provided, which includes, by weight: 38 to 42 parts of cowhide, 38 to 42 parts of gelatin, 14 to 16 parts of rice, 6 to 8 parts of glycerol, 0.4 to 0.6 parts of Ascophyllum nodosum, 0.1 to 0.5 parts of citric acid, 0.2 to 0.9 parts of sodium hexametaphosphate, and 0.005 to 0.05 parts of tea polyphenols.

[0010] Optionally, the cowhide is crushed and ground into cowhide powder that can pass through a 10-mesh sieve. After sieving, the cowhide powder has no obvious particles and agglomerates, and subsequent rehydration processing is more uniform and delicate, which facilitates material mixing.

[0011] Optionally, the moisture content of the gelatin is not higher than 14%. If the moisture content is too high, the gelatin is easily oxidized and absorbs moisture to form lumps. The gelatin protein content on a dry basis is not less than 95%. The gelatin raw material used is of high purity and excellent quality, which can ensure a high protein content in the product.

[0012] Optionally, the moisture content of the dog tooth-cleaning collagen chew product is 25-30%. Within this moisture content range, the product has a relatively moderate hardness, and has the advantages of high toughness and good elasticity, which can achieve a good tooth-cleaning effect. If the moisture content is too high, the product is easy to deteriorate, the shelf life is short, and the hardness is too low and the toughness is poor. If the moisture content is too low, the product has a large hardness and poor elasticity. Regardless of whether the moisture content is too high or too low, the texture of the product cannot achieve a good chewing effect. Therefore, the moisture content of the product is controlled to be 25-30%. Within this moisture content range, the product has a moderate hardness, strong bite resistance, is easy to digest, and has a good tooth-cleaning effect.

[0013] According to another aspect of the present application, a method for preparing the above-mentioned tooth-cleaning collagen chew for dogs is provided, the preparation method comprising:

[0014] 1) Pretreatment of cowhide and gelatin: Crush, grind and filter the cowhide to obtain cowhide powder for later use; soak gelatin in water and heat until completely dissolved for later use;

[0015] 2) Mixing the raw materials under vacuum conditions: weigh the raw materials and use a dough mixer to pre-mix them, and transfer the pre-mixed materials to a vacuum tumbling machine for tumbling under vacuum conditions;

[0016] 3) Baking after extrusion: The mixed materials are extruded by a screw extruder and cut into suitable product sizes, and then baked in an oven to control the moisture content of the product to be no higher than 30%;

[0017] 4) Sterilization treatment: sterilize the baked product to obtain the tooth-cleaning collagen chew for dogs.

[0018] Optionally, the cowhide powder obtained by filtering in step 1) can pass through a 10-mesh sieve.

[0019] Optionally, in the gelatin soaking operation in step 1), 80-90 wt% of water is added to the gelatin raw material, and the gelatin particles become transparent light yellow to amber. At this time, the gelatin rehydration degree is uniform and thorough. If the water content is too high, it will affect the subsequent processing and molding of the product. If the water content is too low, the uneven gelatin rehydration will affect the uniformity of subsequent raw material mixing.

[0020] Optionally, in step 2), the dough is kneaded for 20 to 40 minutes at a stirring rod speed of 40 to 80 rpm in the dough mixer, and the dough mixer premixes the raw materials so that they are evenly mixed for subsequent processing; and / or,

[0021] The mixture was tumbled in a vacuum tumbling machine at a vacuum degree of 0.01 to 0.06 MPa and room temperature at a speed of 25 to 45 rpm for 20 to 40 minutes.

[0022] In the scheme of the present application, step 2) mixes the raw materials under vacuum conditions, so that the physical structure of the product is bulky, which is conducive to the absorption of auxiliary materials. In addition, the product will not oxidize and deteriorate under vacuum conditions. The use of a vacuum tumbling machine has good rolling performance, which significantly improves the quality and effect of the product. If the vacuum degree is insufficient, the physical structure of the prepared product is poorly bulky, which is not conducive to the absorption of auxiliary materials and affects the quality of the product. If the tumbling time is too short, the auxiliary materials are not completely absorbed, which also affects the quality of the product. The above-mentioned preparation scheme of the present application is compatible with a wide range of equipment, saves time and cost, and can also effectively improve product quality.

[0023] Optionally, the screw extruder in step 3) is a single screw extruder with zoned heating, and the molding temperature is 70-120° C. Within this temperature range, the raw material melts into a semi-fluid for easy extrusion molding. If the temperature is too high, the raw material is completely melted and cannot be molded. If the temperature is too low, the raw material is solid and the discharge is uneven, and the molding effect is poor.

[0024] Optionally, the temperature is controlled not to exceed 70°C during the oven baking process. If the temperature is too high, the effective ingredients in the Ascophyllum nodosum will be easily inactivated, and the product will easily melt and deform. If the temperature is too low, a longer baking time is required, and the product will easily deteriorate.

[0025] Optionally, in step 4), the product is sterilized by irradiation at a dose of 5 to 10 kGy. Compared with traditional technologies such as thermal sterilization and chemical preservation, irradiation technology can effectively kill various pathogenic microorganisms in food without changing the original color, aroma, and shape of the product, and has the advantages of small nutritional loss and no drug residue, which can improve the quality of pet food.

[0026] If the irradiation dose is too small, a good sterilization effect cannot be achieved, and if the irradiation dose is too large, it will affect the sensory state of the product such as color and flavor, and affect palatability. In the present application scheme, the use of 5-10kGy irradiation can significantly kill the microorganisms in the product, and has little effect on the main component of the product, water, nutrients, mineral amino acid content, ash content, and sensory quality, and the color and flavor of the product will not be significantly affected, and the dog's acceptability is good.

[0027] The beneficial effects of this application include but are not limited to:

[0028] 1. According to the dog tooth-cleaning collagen chew and its preparation method of the present application, the provided dog tooth-cleaning collagen chew is easy to digest, has good toughness and chewing resistance, has moderate hardness and strong bite resistance, and can remove and control dental plaque and tartar through physical and mechanical friction.

[0029] 2. According to the dog tooth-cleaning collagen chew and its preparation method of the present application, with Ascophyllum nodosum and tea polyphenols, citric acid is also added to synergistically inhibit the growth of bacteria, and sodium hexametaphosphate is added to chelate calcium in saliva, thereby effectively reducing the mineralization of dental plaque to form tartar.

[0030] 3. According to the dog tooth-cleaning collagen chew and its preparation method of the present application, the present invention solves the problem that traditional cowhide molar sticks and tooth-cleaning bones are hard and difficult to digest by rationally selecting raw materials and improving production processes. It can remove and control dental plaque and tartar through multiple ways and has important market economic value.

[0031] 4. According to the dog tooth-cleaning collagen chew and its preparation method of the present application, the production process of the production process is simple to operate, the facilities and equipment occupy a small area, and it can save labor costs while improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0033] Figure 1 This is a sensory comparison diagram of dog teeth before and after the test involved in this application (A: dog teeth before the test; B: dog teeth after the test);

[0034] Figure 2 This is a graph showing the change in the total number of bacterial colonies in the oral cavity of a dog before and after chewing the dog's dental cleaning collagen chew involved in the present application (A: graph showing the total number of bacterial colonies in the oral cavity of a dog before chewing the product; B: graph showing the total number of bacterial colonies in the oral cavity of a dog after chewing the product);

[0035] Figure 3 This is a product picture of the dog tooth-cleaning collagen chew involved in Example 1 of the present application. DETAILED DESCRIPTION

[0036] The present application is described in detail below in conjunction with examples, but the present application is not limited to these examples. Unless otherwise specified, the raw materials and catalysts in the examples of the present application are purchased through commercial channels.

[0037] The present application scheme is described below through specific embodiments.

[0038] Example 1

[0039] A tooth-cleaning collagen chew for dogs comprises the following raw materials in parts by mass: 42 parts of cowhide, 38 parts of coarse gelatin particles (extracted from cowhide and cow bones), 14 parts of rice, 6 parts of glycerol, 0.4 parts of Ascophyllum nodosum, 0.1 parts of citric acid, 0.2 parts of sodium hexametaphosphate, and 0.005 parts of tea polyphenols.

[0040] The preparation method comprises the following steps:

[0041] 1) Acceptance, processing and pretreatment of raw materials

[0042] 1.1) Raw material acceptance: In the sensory test, the cowhide pieces should be natural white or milky white with uniform color, fragrant and odorless, and free of impurities such as hair; in the sensory test, the coarse gelatin particles should be light yellow to yellow, translucent, slightly shiny particles or flakes, and the aqueous solution should be odorless after being dissolved in a 70℃ water bath, and the moisture content of the particles should be ≤14%, and the protein content should be ≥95% (on a dry basis);

[0043] 1.2) Raw material processing and pretreatment: The cowhide pieces that have passed the inspection in the previous step are crushed into cowhide particles with a diameter of 15 to 20 mm by a granulator, and then ground into cowhide powder that can pass through a 10-mesh sieve by a grinder; 80% of the total weight of the raw materials are added to the gelatin coarse particles that have passed the inspection in the previous step to fully soak and pre-dissolve, and heated in an electric heating sandwich pot until completely dissolved for use;

[0044] 2) Mix the raw materials under vacuum conditions

[0045] The pretreated raw materials were weighed according to the mass percentage, and the raw materials were pre-mixed using a fully automatic dough mixer. The dough was kneaded for 40 minutes at a stirring rod speed of 40 rpm. The pre-mixed materials were then transferred to a vacuum tumbling machine and tumbled for 40 minutes at a vacuum degree of 0.01 MPa, a temperature of 25°C, and a tumbling speed of 25 r / min.

[0046] 3) Extrusion molding

[0047] The materials mixed evenly in the previous step are heated in different zones by a single screw extruder. The temperatures are 100°C in zone I, 120°C in zone II, 120°C in zone III, 95°C in zone IV, and 70°C in zone V. The feeding speed is 40HZ and the screw speed is 45HZ for extrusion molding. When the discharge is uniform and the product surface is smooth and delicate, the conveyor belt is opened to receive the material. The cutting speed of the conveyor belt at the discharge port is adjusted according to the required product size to control the product length at 10.3-10.7cm.

[0048] 4) Product baking

[0049] The products obtained in the previous step are preliminarily selected, and the products with uniform length and no foreign matter, impurities or defects on the surface are evenly placed on the mesh screen, and baked in a hot air circulation oven at 65°C for 8 hours to control the moisture content of the products to 25-30%;

[0050] 5) Irradiation sterilization

[0051] Use a dose of 5 to 10 kGy to sterilize the product by irradiation;

[0052] 6) Product quality testing and packaging

[0053] The product after irradiation sterilization in the previous step is subjected to quality inspection. The microorganism Salmonella in the product shall not be detected, and there shall be no foreign matter such as metal, hair, plastic film, etc. The product color and length shall be uniform, the moisture content shall be 25-30%, and the weight of a single piece shall be 10.5-11g. After passing the quality inspection, the product shall be packaged. Figure 3 shown.

[0054] Example 2

[0055] A tooth-cleaning collagen chew for dogs comprises the following raw materials in parts by mass: 38 parts of cowhide, 42 parts of coarse gelatin particles (extracted from cowhide and cow bones), 16 parts of rice, 8 parts of glycerol, 0.6 parts of Ascophyllum nodosum, 0.5 parts of citric acid, 0.9 parts of sodium hexametaphosphate, and 0.05 parts of tea polyphenols.

[0056] The preparation method comprises the following steps:

[0057] 1) Acceptance, processing and pretreatment of raw materials

[0058] 1.1) Raw material acceptance: In the sensory test, the cowhide pieces should be natural white or milky white with uniform color, fragrant and odorless, and free of impurities such as hair; in the sensory test, the coarse gelatin particles should be light yellow to yellow, translucent, slightly shiny particles or flakes, and the aqueous solution should be odorless after being dissolved in a 70℃ water bath, and the moisture content of the particles should be ≤14%, and the protein content should be ≥95% (on a dry basis);

[0059] 1.2) Raw material processing and pretreatment: The cowhide pieces that have passed the inspection in the previous step are crushed into cowhide particles with a diameter of 15 to 20 mm by a pellet machine, and then ground into cowhide powder that can pass through a 10-mesh sieve by a grinder; 90% of the total weight of the raw materials is added to the gelatin coarse particles that have passed the inspection in the previous step to fully soak and pre-dissolve, and heated in an electric heating sandwich pot until completely dissolved for use;

[0060] 2) Mix the raw materials under vacuum conditions

[0061] The pretreated raw materials were weighed according to the mass percentage, and the raw materials were pre-mixed using a fully automatic dough mixer. The dough was kneaded for 20 minutes at a stirring rod speed of 80 rpm. The pre-mixed materials were then transferred to a vacuum tumbling machine and tumbled for 20 minutes at a vacuum degree of 0.06 MPa, a temperature of 25°C, and a tumbling speed of 45 r / min.

[0062] 3) Extrusion molding

[0063] The materials mixed evenly in the previous step are heated in different zones by a single screw extruder. The temperatures are 100°C in zone I, 120°C in zone II, 120°C in zone III, 95°C in zone IV, and 70°C in zone V. The feeding speed is 30HZ and the screw speed is 20HZ for extrusion molding. When the discharge is uniform and the product surface is smooth and delicate, the conveyor belt is opened to receive the material. The cutting speed of the conveyor belt at the discharge port is adjusted according to the required product size to control the product length at 10.3-10.7cm.

[0064] 4) Product baking

[0065] The products obtained in the previous step are preliminarily selected, and the products with uniform length and no foreign matter, impurities or defects on the surface are evenly placed on the mesh screen, and baked in a hot air circulation oven at 65°C for 6 hours to control the moisture content of the products to 25-30%;

[0066] 5) Irradiation sterilization

[0067] Use a dose of 5 to 10 kGy to sterilize the product by irradiation;

[0068] 6) Product quality testing and packaging

[0069] Carry out quality inspection on the products sterilized by irradiation in the previous step. No microorganism Salmonella shall be detected in the products, and there shall be no foreign matter such as metal, hair, plastic film, etc. The color and length of the products shall be uniform, the moisture content shall be 25-30%, and the weight of a single piece shall be 10.5-11g. After passing the quality inspection, the products shall be packaged.

[0070] Comparative Example 1

[0071] This comparative example is substantially the same as Example 1, except that citric acid is replaced by an equal amount of lactic acid.

[0072] Comparative Example 2

[0073] This comparative example is substantially the same as Example 1, except that citric acid is not contained.

[0074] Comparative Example 3

[0075] This comparative example is substantially the same as Example 1, except that it does not contain sodium hexametaphosphate.

[0076] Comparative Example 4

[0077] This comparative example is basically the same as Example 1, except that tea polyphenols are replaced by an equal amount of ordinary green tea extract, and Ascophyllum nodosum is replaced by an equal amount of kelp powder.

[0078] Test Example 1 Hardness, elasticity and toughness test

[0079] Texture is a property of food in terms of taste, which is determined by the mutual influence of the physical structure and components of the food, and is divided into aspects such as hardness, elasticity and toughness. Hardness refers to the degree of resistance shown by food when subjected to external force. Measuring hardness can help evaluate the chewiness and taste of food. Elasticity refers to the ability of food to recover after being subjected to external force. Food with good elasticity can return to its original shape after being subjected to force. Toughness refers to the ability of food to stretch and deform without breaking when subjected to external force. Food with higher toughness can stretch and deform after being subjected to force, while food with low toughness will easily break.

[0080] The hardness, elasticity and toughness of the commercially available chewing gum and the collagen chews for dogs in Examples 1 and 2 were tested using a texture analyzer. The results are shown in Table 1 below.

[0081] Table 1 Hardness, elasticity and toughness test results

[0082] sample Hardness(N) elasticity(%) Toughness(N.mm) Commercially available chewing gum teether 81.20±14.00 15.20±6.20 / Example 1 52.44±5.71 86.02±1.20 360.24±4.08 Example 2 50.28±5.67 88.11±1.08 349.77±3.95

[0083] According to the results in Table 1, the dog tooth-cleaning collagen chews in the embodiments have the advantages of moderate hardness, high toughness and good elasticity compared with commercially available chewing gum and molar sticks. In particular, the dog tooth-cleaning collagen chews of the present application have moderate hardness and strong bite resistance, provide sufficient mechanical friction and have good tooth-cleaning effect, and can also meet the dog's gnawing and chewing needs.

[0084] The gelatin content in the prior art tooth-cleaning chewing products is low. For example, the main component of patent CN 116762906 A is starch, and the amount of gelatin added is small. Patent CN 107927407 B also has a low gelatin content. In the present application, a large amount of gelatin is used in combination with cowhide in a suitable ratio, which can adjust the hardness, toughness and elasticity of the final product. The gelatin provides elasticity under suitable conditions, and the cowhide provides appropriate hardness and toughness, so that the product of the present application has the advantages of moderate hardness, high toughness and good elasticity. In addition, the present application uses rice, while the prior art uses corn starch, flour, etc. The rice in the present application is not ground during processing but added in the form of particles, which can also improve the hardness and toughness of the product and have a good granular feel, and can better meet the biting and chewing needs of dogs. At the same time, it can achieve a better tooth-cleaning effect with active ingredients.

[0085] If the proportion of gelatin in the total amount of raw materials in the formula is too high, the elasticity of the product will increase slightly, but the hardness and toughness will decrease. If the proportion of gelatin is too low, the elasticity of the product will be insufficient. In addition, if the proportion of cowhide in the total amount of raw materials in the formula is too high, the hardness of the product will increase significantly, but the toughness and elasticity will decrease. If the proportion of cowhide is too low, the hardness and toughness will be insufficient. If the proportion of gelatin and cowhide in the product is not appropriate, a good chewing effect cannot be achieved, and it is impossible to ensure that the product has moderate elasticity while having good toughness and appropriate hardness, and the physical friction and cleaning effect on the tooth surface is poor.

[0086] Test Example 2: Plaque Index and Calculus Index of Test Dogs

[0087] Dogs of similar age, breed, and health status were selected. The plaque index (Plaque Index) and calculus index (Calcuil Index) of the test dogs were recorded and evaluated before the start of the experiment. Then, the dogs were continuously fed 30 g / d of the tooth-cleaning collagen chew obtained in Example 1. After feeding for 14 days, the plaque index and calculus index of the test dogs were observed and recorded again. The results are shown in Table 4 below, wherein Table 2 and Table 3 are the plaque index scoring standards and the calculus index scoring standards, respectively.

[0088] Table 2 Scoring criteria for dental plaque index

[0089] score standard PI0 No visible plaques PI1 Small, scattered patches of plaque covering less than 1 / 3 of the buccal tooth surface PI2 Plaque covers 1 / 3 to 2 / 3 of the buccal tooth surface PI3 Plaque covers more than 1 / 3 to 2 / 3 of the buccal tooth surface

[0090] Table 3 Scoring criteria for dental calculus index

[0091]

[0092] Table 4 Effect of dental plaque index and calculus index on dogs tested in Example 1

[0093]

[0094]

[0095] The comparison of dog tooth sensory perception before and after the product test of Example 1 is shown in the figure below: Figure 1 As shown, it can be seen intuitively that after using the tooth-cleaning collagen chew for dogs in Example 1 for 14 days, the dental plaque adhesion on the surface of the dog's teeth is significantly reduced. Further, combined with the results in Table 4, it can be seen that after the test dogs used the tooth-cleaning collagen chew for dogs of the present application, the dental plaque index and the dental calculus index were significantly improved, and the present application has a good effect of cleaning teeth and improving the oral environment of dogs.

[0096] The results of the dogs tested with the products in Comparative Examples 3 and 4 are shown in Tables 5 and 6 below, respectively.

[0097] Table 5 Effect of dental plaque index and calculus index on dogs in comparative example 3

[0098]

[0099] Table 6 Effect of dental plaque index and calculus index on dogs in comparative example 4

[0100]

[0101]

[0102] According to the results in Table 5 and Table 6, the teeth cleaning effects of Comparative Examples 3 and 4 on dogs are inferior to those of the present application.

[0103] Test Example 3 Total number of oral bacteria before and after chewing

[0104] Dogs of similar age, breed, and health status were selected, and samples were collected from the tongue and gums of the dogs using a sterilized oral swab before and after chewing the dog tooth-cleaning collagen chew in Example 1. After collection, the swab was quickly placed in a test tube containing a stabilizing solution, which was sealed and sent to the laboratory for testing the total number of oral bacterial colonies. The results are shown in Table 7 below.

[0105] Table 7 Effect of total oral bacterial counts on dogs before and after chewing in Example 1

[0106]

[0107] Changes in the total number of oral bacteria in dogs before and after chewing collagen chews for dogs Figure 2 As shown, it can be seen intuitively that the total number of oral bacteria in the oral cavity is significantly reduced after using the tooth-cleaning collagen chew for dogs in Example 1. Further, combined with the results in Table 7, it can be seen that the tooth-cleaning collagen chew provided by the present application has a good antibacterial effect and can improve the oral health of dogs.

[0108] Test Example 4 Pepsin digestibility determination

[0109] Compare the pepsin digestibility of canine dental collagen chews and commercial chew sticks. GB / T17811-2008 standard is applicable to the determination of pepsin digestibility of all animal protein feeds, so the test operation was carried out according to the test method specified in GB / T 17811-2008, and the test results are shown in Table 8 below.

[0110] Table 8 Pepsin digestibility of samples

[0111] sample Pepsin digestibility % Example 1 Collagen chew for dog teeth cleaning 97.20％ Commercially available chewing gum teether 57.52％

[0112] According to the results in Table 8, the tooth-cleaning collagen chews provided by the present application are easier to digest and can significantly reduce the incidence of vomiting and indigestion in dogs after consumption.

[0113] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A tooth-cleaning collagen chew for dogs, characterized in that: The ingredients include, by weight: 38-42 parts of cowhide, 38-42 parts of gelatin, 14-16 parts of rice, 6-8 parts of glycerol, 0.4-0.6 parts of Ascophyllum nodosum, 0.1-0.5 parts of citric acid, 0.2-0.9 parts of sodium hexametaphosphate, and 0.005-0.05 parts of tea polyphenols.

2. The tooth-cleaning collagen chew for dogs according to claim 1, characterized in that: The cowhide is crushed and ground to produce cowhide powder that can pass through a 10-mesh sieve.

3. The tooth-cleaning collagen chew for dogs according to claim 1, characterized in that: The moisture content of the gelatin is not higher than 14%, and the protein content on a dry basis is not lower than 95%.

4. The tooth-cleaning collagen chew for dogs according to claim 1, characterized in that: The moisture content of the dog tooth-cleaning collagen chew product is 25-30%.

5. The method for preparing the tooth-cleaning collagen chew for dogs according to any one of claims 1 to 4, characterized in that: The preparation method comprises: 1) Pretreatment of cowhide and gelatin: Crush, grind and filter the cowhide to obtain cowhide powder for later use; soak gelatin in water and heat until completely dissolved for later use; 2) Mixing the raw materials under vacuum conditions: weigh the raw materials and use a dough mixer to pre-mix them, and transfer the pre-mixed materials to a vacuum tumbling machine for tumbling under vacuum conditions; 3) Baking after extrusion: The mixed materials are extruded by a screw extruder and cut into suitable product sizes, and then baked in an oven to control the moisture content of the product to be no higher than 30%; 4) Sterilization treatment: sterilize the baked product to obtain the tooth-cleaning collagen chew for dogs.

6. The method for preparing the tooth-cleaning collagen chew for dogs according to claim 5, characterized in that: The cowhide powder obtained by filtering in the step 1) can pass through a 10-mesh sieve.

7. The method for preparing the tooth-cleaning collagen chew for dogs according to claim 5, characterized in that: In the step 1), 80-90 wt % of water is added to the gelatin raw material during the gelatin soaking process.

8. The method for preparing the tooth-cleaning collagen chew for dogs according to claim 5, characterized in that: In the step 2), the dough is kneaded for 20 to 40 minutes at a stirring rod speed of 40 to 80 rpm in the dough mixer; and / or, The mixture was tumbled in a vacuum tumbling machine at a vacuum degree of 0.01 to 0.06 MPa and room temperature at a speed of 25 to 45 rpm for 20 to 40 minutes.

9. The method for preparing the tooth-cleaning collagen chew for dogs according to claim 5, characterized in that: In step 3), the screw extruder is a single screw extruder with zoned heating, and the molding temperature is 70-120° C.; and / or, The temperature was controlled not to exceed 70°C during the baking process.

10. The method for preparing the tooth-cleaning collagen chew for dogs according to claim 5, characterized in that: In the step 4), the product is irradiated and sterilized with a dose of 5 to 10 kGy.

Images