A sampling device and method for detecting salmonella on egg surface

Abstract

The present invention provides a sampling device and a detection method for detecting salmonella on egg surfaces, comprising a detection platform, a plurality of egg slots are arranged on the left and right side tables of the detection platform, the plurality of egg slots on both sides are located on the same straight line, the bottoms of the plurality of egg slots are provided with negative pressure suction holes, a rotating column is rotatably connected at the center of the detection platform, a rotating arm is fixedly connected to the top of the rotating column, a first lifting plate and a second lifting plate that can be lifted and lowered are respectively installed at the bottoms of the left and right sides of the rotating arm, the tops of the first lifting plate and the second lifting plate are rotatably connected to a plurality of telescopic shafts, and a driving mechanism that can synchronously drive the plurality of telescopic shafts to rotate is installed in the rotating arm. The device can simultaneously complete the sampling process of the surfaces of a plurality of eggs and the interiors of a plurality of eggs, and can also automatically complete the pathogen cultivation process during the detection process.

Description

Technical Field

[0001] The invention relates to the technical field of egg detection, and in particular to a sampling device and a detection method for salmonella on the surface of eggs. Background Art

[0002] Eggs contain a large amount of vitamins, minerals and proteins with high biological value. They are one of the foods commonly consumed by humans. However, since eggs and chicken manure are discharged from the same outlet, the surface of eggs is very likely to be contaminated with Salmonella, which poses a considerable threat to the safety of eggs. Therefore, when using eggs for food processing, it is necessary to conduct biochemical testing on the food samples to determine whether Salmonella is carried to ensure food safety.

[0003] The patent with publication number CN114354602B discloses a biochemical detection device for egg food production safety with early warning prompts, including a detection table and a placement rack, a placement rack is installed on the top of the detection table, three groups of sample observation racks are placed on the top of the detection table, a transparent cover is installed on the inner wall of the groove through an axle, a No. 1 liquid storage tank is provided on the bottom wall of the groove, a background light is installed inside the sample observation rack, a No. 2 liquid storage tank is provided on the top of the sample observation rack, an alarm light is installed on the top of the sample observation rack; a culture test tube is installed inside the limit hole. During the detection process of this device, cotton swabs are needed to be used to manually sample the surface of the eggs. For some eggs with clean surfaces, it is necessary to detect whether there is salmonella inside the eggs, and the eggs need to be broken and sampled. The sampling process is relatively troublesome, and the degree of automation of the culture process of pathogen detection is also low. Summary of the invention

[0004] In view of the above problems, the present invention provides a sampling device for detecting Salmonella on the surface of eggs, which can simultaneously complete the sampling process of multiple egg surfaces and multiple egg interiors, and can also automatically complete the pathogen cultivation process during the detection process.

[0005] To solve the above problems, the technical solution adopted by the present invention is:

[0006] A sampling device for detecting salmonella on egg surfaces comprises a detection platform, wherein a plurality of egg slots are arranged on the table tops on the left and right sides of the detection platform, the plurality of egg slots on the two sides are located on the same straight line, and negative pressure suction holes are arranged at the bottoms of the plurality of egg slots, a rotating column is rotatably connected at the center of the detection platform, a rotating arm is fixedly connected to the top of the rotating column, a first lifting plate and a second lifting plate which can be lifted and lowered are respectively installed at the bottoms of the left and right sides of the rotating arm, the tops of the first lifting plate and the second lifting plate are rotatably connected to a plurality of telescopic shafts, a driving mechanism which can synchronously drive the plurality of telescopic shafts to rotate is installed in the rotating arm, and a driving mechanism which is one-to-one with the plurality of telescopic shafts on the left side is installed at the bottom of the first lifting plate There are multiple turntables connected to the left side, and the multiple turntables correspond to the multiple egg slots on the left side one by one. A pair of cotton swabs are elastically inserted at the bottom of the multiple turntables. A plurality of drill rods that are connected to the multiple telescopic shafts on the right side are installed at the bottom of the second lifting plate. Expandable elastic sheets are embedded on both sides of the drill rods. Suction holes are provided on both sides of the drill bit of the drill rods. A plurality of pumping mechanisms connected to the suction holes of the corresponding drill rods are installed in the second lifting plate. Culture chambers are provided on the front and back sides of the detection platform. A liftable loading plate is installed in the culture chamber. Three groups of placement slots are provided on the loading plate. The number of the placement slots in each group is consistent with the number of multiple egg slots on one side. A cleaning slot is also radially provided on the table top of the detection platform.

[0007] Preferably, a negative pressure pump is installed on the side wall of the detection table, the suction end of the negative pressure pump is connected to a suction pipe extending into the detection table, the multiple negative pressure holes are connected to the suction pipe, and the notch of the egg trough is installed with a sealing gasket.

[0008] Preferably, first electric push rods are fixedly mounted on the tops of both sides of the rotating arm, and telescopic ends of the two first electric push rods are fixedly connected to the first lifting plate and the second lifting plate respectively.

[0009] Preferably, the driving mechanism includes a transmission cavity arranged in the rotating arm, a driving shaft is rotatably connected in the transmission cavity, the telescopic ends of the multiple telescopic shafts extend into the transmission cavity, the driving shaft and the extended ends of the multiple telescopic shafts are connected through a bevel gear pair, and a motor that can drive the driving shaft to rotate is installed at one end of the rotating arm.

[0010] Preferably, the bottom of both sides of the turntable are provided with a slide groove, a slider is elastically connected to the slide groove through a first spring, the bottom of the slider is provided with a socket for inserting the wooden handle end of the cotton swab, and a rubber sleeve that resists the wooden handle end is installed in the socket.

[0011] Preferably, a pair of receiving grooves and a pair of vertical grooves are provided on both sides of the drill rod, the lower end of the elastic sheet is fixedly connected to the inner bottom of the corresponding receiving groove, the upper end of the elastic sheet is fixedly connected to a stop block, the stop block is slidably connected in the corresponding receiving groove, a stop rod is elastically connected in the vertical groove through a second spring, the lower end of the stop rod extends into the corresponding side receiving groove and is fixedly connected to the stop block, the top end of the drill rod is sleeved with an electric coil electrically connected to the second spring, and a brush in contact with the electric coil and connected to an external controller is installed on the top of the second lifting plate.

[0012] Preferably, the pumping mechanism includes a plurality of negative pressure chambers arranged in the second lifting plate, the upper end of the drill rod passes through the negative pressure chamber and is fixedly connected to the corresponding telescopic shaft, a rotation seal is arranged at the connection between the drill rod and the negative pressure chamber, a vertically extending suction pipe connected to a pair of suction holes is provided in the drill rod, the upper end of the suction pipe is connected to a connecting hole connected to the negative pressure chamber, and a suction assembly is also installed in the second lifting plate.

[0013] Preferably, the suction assembly includes a plurality of pump fluid chambers arranged in the second lifting plate, a piston is provided in the pump fluid chamber, the pump fluid chamber is connected to the corresponding negative pressure chamber through a connecting pipe, a second electric push rod is fixedly installed at one end of the second lifting plate, the telescopic end of the second electric push rod is fixedly connected to a pull rod extending to the plurality of pump fluid chambers, and the plurality of pistons are fixed on the pull rod.

[0014] Preferably, a cylinder is installed at the bottom of the culture chamber, the telescopic end of the cylinder is fixedly connected to a bottom plate, the bottom plate is fixed to a carrier plate via a rubber pad, a vibration motor is installed on the bottom plate of the carrier plate, and a door is installed at the opening of the culture chamber.

[0015] The present invention also provides a method for detecting salmonella on the surface of eggs, comprising the following specific steps:

[0016] S1, selecting a plurality of eggs with dirty surfaces and placing them in a plurality of egg slots on the left, and selecting a plurality of eggs with clean surfaces and placing them in a plurality of egg slots on the right, and fixing them by negative pressure adsorption;

[0017] S2, start the first electric push rod on the left side to drive the first lifting plate to move downward, and the multiple pairs of cotton swabs are pressed against the two sides of the corresponding dirty eggs under the action of the first spring, and the multiple turntables are synchronously driven to rotate through the driving mechanism, thereby driving the multiple pairs of cotton swabs to rotate around the surface of the egg for multiple circles to complete the sampling of surface dirt;

[0018] S3, start the first electric push rod on the right side to drive the second lifting plate to move downward, and then drive the multiple drill rods to rotate through the driving mechanism, drill into multiple clean eggs from the top, and then energize the second spring through the controller to make it contract and squeeze the elastic sheet, so that it expands and stirs inside the egg, mixes the egg yolk and egg liquid, and then samples through the pump mechanism;

[0019] S4, pour the prepared enrichment solution into a culture dish and place it in a group of culture tanks, and the rotating arm can be lifted and lowered to insert the sampled cotton swab and the drill rod into the enrichment solution on the corresponding side, and the dirty sample on the cotton swab directly enters the enrichment solution, and the egg liquid in the drill rod is squeezed into the corresponding enrichment solution through the pump mechanism, and the carrier plate is lowered into the culture chamber for culture;

[0020] S5, pour the prepared selective enrichment solution into a culture dish and place it in a group of culture tanks, use the drill rod and pump mechanism to extract the enrichment solution in S4 and squeeze it into the selective enrichment solution for culture;

[0021] S6, pour the prepared culture medium into a culture dish and place it in a group of culture tanks, use the drill rod and pump mechanism to extract the selective bacterial enrichment solution in S5 and squeeze it onto the culture medium, the drill rod can be rotated to achieve the streaking function, and enter the culture room for culture;

[0022] S7. Take out the culture medium in S6, and observe the morphology and color of the bacteria in the culture medium under a microscope to determine whether Salmonella is present.

[0023] The beneficial effects of the present invention are:

[0024] 1. By installing multiple egg troughs, negative pressure holes and negative pressure pumps, the multiple egg troughs on the left can be used to place multiple eggs with dirty surfaces, and the multiple egg troughs on the right can be used to place multiple eggs with clean surfaces. The sealing gaskets can resist the outer periphery of the eggs, so that the egg trough is in a closed space. Start the negative pressure pump, and the air in the egg trough can be simultaneously sucked through the suction pipe and multiple negative pressure holes, thereby adsorbing the eggs, which is convenient for the subsequent surface and internal sampling process.

[0025] 2. By installing the driving mechanism, the telescopic shaft, the first lifting plate, multiple turntables and multiple pairs of cotton swabs, the first electric push rod on the left is started to drive the first lifting plate to move downward. Under the action of the first spring, the multiple pairs of cotton swabs are pressed against the two sides of the corresponding dirty eggs. The multiple turntables are synchronously driven to rotate through the driving mechanism and the telescopic shaft, thereby driving the multiple pairs of cotton swabs to rotate multiple circles around the surface of the egg to complete the sampling of surface dirt.

[0026] 3. By installing the driving mechanism, the telescopic shaft, the second lifting plate, the drill rod and the pumping mechanism, the first electric push rod on the right is started to drive the second lifting plate to move downward, and then the driving mechanism and the telescopic shaft are used to drive the multiple drill rods to rotate, and drill into multiple clean eggs from the top, and then the second spring is energized through the controller to contract and squeeze the elastic sheet, so that it expands and stirs inside the egg, and the egg yolk and egg liquid are mixed evenly, and then sampling is carried out through the pumping mechanism, and the second electric push rod is started, and the multiple pistons are driven to move by the pull rod to generate negative pressure in the negative pressure chamber, and then suction is carried out through the connecting hole, the liquid pipette and the liquid suction hole. The second electric push rod is reset to squeeze out the liquid, and then the suction and extrusion process of the egg liquid, the enrichment liquid and the selective enrichment liquid can be carried out.

[0027] 4. By installing a rotating arm, two culture chambers, a carrier plate and three groups of placement slots, the configured enrichment solution, selective enrichment solution and culture medium can be poured into multiple culture dishes respectively, and then placed in the corresponding multiple groups of culture slots respectively. The rotating arm can be lifted and lowered to insert the sampled cotton swab and drill rod into the enrichment solution on the corresponding side. The dirty sample on the cotton swab directly enters the enrichment solution, and the egg liquid in the drill rod is squeezed into the corresponding enrichment solution through the pump mechanism. After cultivation, the enrichment solution is extracted by the drill rod and the pump mechanism and squeezed into the selective enrichment solution for cultivation. The selective enrichment solution is extracted by the drill rod and the pump mechanism and squeezed onto the culture medium. The drill rod can realize the streaking function by rotating, and the culture chamber can realize constant temperature cultivation. After the enrichment solution and the selective enrichment solution are cultivated, the vibration motor can be started to drive the carrier plate to vibrate, and mixing can be achieved before taking the liquid.

[0028] 5. This device can realize rapid sampling and automated culture process, and use a microscope to observe the morphology and color of the bacteria in the culture medium to determine whether Salmonella is present, with a fast detection speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A perspective view of the present invention;

[0030] Figure 2 is a cross-sectional view of the present invention;

[0031] Figure 3 A top view of the detection platform proposed by the present invention;

[0032] Figure 4 for Figure 2 A schematic diagram of the structure enlargement at point A;

[0033] Figure 5 This is a schematic diagram of the expansion state of the elastic sheet proposed by the present invention;

[0034] Figure 6 A cross-sectional view of a turntable provided by the present invention;

[0035] Figure 7 A front view of the base plate proposed by the present invention;

[0036] Figure 8 This is a top cross-sectional schematic diagram of a second lifting rod plate proposed by the present invention;

[0037] Fig. 9 This is a schematic diagram of the sampling state proposed by the present invention.

[0038] In the figure: 1 testing table, 2 negative pressure pump, 3 suction pipe, 4 negative pressure hole, 5 egg tank, 6 sealing gasket, 7 rotating column, 8 motor, 9 first electric push rod, 10 rotating arm, 11 transmission cavity, 12 driving shaft, 13 bevel gear pair, 14 rubber pad, 15 telescopic shaft, 16 first lifting plate, 17 turntable, 18 cotton swab, 19 drill rod, 20 second electric push rod, 21 second lifting plate, 22 slide groove, 23 first spring, 24 slider, 25 rubber sleeve, 26 negative pressure cavity, 27 connecting hole, 28 suction pipe, 29 vertical groove, 30 second spring, 31 push rod, 32 push block, 33 storage groove, 34 elastic sheet, 35 suction hole, 36 brush, 37 room door, 38 cleaning tank, 39 culture room, 40 loading plate, 41 placement tank, 42 ​​piston, 43 pull rod, 44 pump liquid cavity, 45 cylinder, 46 bottom plate, 47 vibration motor. DETAILED DESCRIPTION

[0039] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0040] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0041] Reference Figure 1-9, a sampling device for detecting salmonella on egg surface, comprising a detection table 1, a plurality of egg slots 5 are arranged on the table tops on the left and right sides of the detection table 1, the plurality of egg slots 5 on both sides are located on the same straight line, the bottoms of the plurality of egg slots 5 are provided with negative pressure suction holes 4, a negative pressure pump 2 is installed on the side wall of the detection table 1, the suction end of the negative pressure pump 2 is connected with a suction pipe 3 extending into the detection table 1, the plurality of negative pressure holes 4 are connected with the suction pipe 3, the notches of the egg slots 5 are installed with sealing gaskets 6, the plurality of egg slots 5 on the left side can be used to place a plurality of eggs with dirty surfaces, the plurality of egg slots 5 on the right side are used to place a plurality of eggs with clean surfaces, the sealing gaskets 6 can resist the periphery of the eggs, so that the egg slots 5 are in a closed space, the negative pressure pump 2 is started, the air in the egg slots 5 can be synchronously sucked through the suction pipe 3 and the plurality of negative pressure holes 4, and then the eggs are adsorbed, which is convenient for the subsequent surface and internal sampling process.

[0042] A rotating column 7 is rotatably connected at the center of the detection platform 1, and a rotating arm 10 is fixedly connected to the top of the rotating column 7. A first lifting plate 16 and a second lifting plate 21 that can be lifted and lowered are respectively installed at the bottom of the left and right sides of the rotating arm 10. First electric push rods 9 are fixedly installed on the tops of both sides of the rotating arm 10. The telescopic ends of the two first electric push rods 9 are respectively fixedly connected to the first lifting plate 16 and the second lifting plate 21. The tops of the first lifting plate 16 and the second lifting plate 21 are rotatably connected to multiple telescopic shafts 15. A rotary shaft 15 that can synchronously drive the multiple telescopic shafts 15 to rotate is installed in the rotating arm 10. The driving mechanism includes a transmission chamber 11 arranged in the rotating arm 10, and a driving shaft 12 is rotatably connected in the transmission chamber 11. The telescopic ends of multiple telescopic shafts 15 all extend into the transmission chamber 11. The driving shaft 12 and the extended ends of the multiple telescopic shafts 15 are all connected through a bevel gear pair 13. A motor 8 that can drive the driving shaft 12 to rotate is installed at one end of the rotating arm 10. The starting motor 8 drives the driving shaft 12 to rotate, and the multiple telescopic shafts 15 are synchronously driven to rotate through multiple sets of bevel gear pairs 13, thereby synchronously driving the multiple turntables 17 and the multiple drill rods 19 to rotate.

[0043] A plurality of turntables 17 are installed at the bottom of the first lifting plate 16, which are connected one by one with the plurality of telescopic shafts 15 on the left side. The plurality of turntables 17 correspond one by one to the plurality of egg slots 5 on the left side. A pair of cotton swabs 18 are elastically inserted at the bottom of the plurality of turntables 17. A slide groove 22 is provided at the bottom of both sides of the turntable 17. A slider 24 is elastically connected in the slide groove 22 through a first spring 23. A socket for inserting the wooden handle end of the cotton swab 18 is provided at the bottom of the slider 24. A rubber sleeve 25 that contacts the wooden handle end is installed in the socket. The plurality of pairs of cotton swabs 18 contact the two sides of the corresponding dirty eggs under the action of the first spring 23. The replacement process can be realized by simply pulling out the wooden handle of the cotton swab and inserting a new cotton swab.

[0044] The bottom of the second lifting plate 21 is equipped with multiple drill rods 19 that are connected one by one with the multiple telescopic shafts 15 on the right side. Expandable elastic sheets 34 are embedded on both sides of the drill rods 19. A pair of receiving grooves 33 and a pair of vertical grooves 29 are provided on both sides of the drill rods 19. The lower end of the elastic sheet 34 is fixedly connected to the inner bottom of the corresponding receiving groove 33, and the upper end of the elastic sheet 34 is fixedly connected to a stop block 32. The stop block 32 is slidably connected in the corresponding receiving groove 33. A stop rod 31 is elastically connected in the vertical groove 29 through a second spring 30. The lower end of the stop rod 31 extends into the corresponding side receiving groove 33 and is fixedly connected to the stop block 32. The top end of the drill rod 19 is sleeved with an electric coil electrically connected to the second spring 30. A brush 36 that contacts the electric coil and is externally connected to the controller is installed on the top of the second lifting plate 21. The second spring 30 is energized by the controller to shrink and then squeeze the elastic sheet 34, so that it expands and stirs inside the egg to mix the yolk and egg liquid.

[0045] Liquid suction holes 35 are provided on both sides of the drill bit of the drill rod 19. A plurality of pumping mechanisms connected to the corresponding liquid suction holes 35 of the drill rod 19 are installed in the second lifting plate 21. The pumping mechanism includes a plurality of negative pressure chambers 26 arranged in the second lifting plate 21. The upper end of the drill rod 19 passes through the negative pressure chamber 26 and is fixedly connected to the corresponding telescopic shaft 15. A rotation seal is provided at the connection between the drill rod 19 and the negative pressure chamber 26. A liquid suction pipe 28 extending vertically and connected to a pair of liquid suction holes 35 is provided in the drill rod 19. The upper end of the liquid suction pipe 28 is connected to a connecting hole 27 connected to the negative pressure chamber 26. A suction assembly is also installed in the second lifting plate 21. The suction assembly ... suction pipe 28 arranged in the first There are multiple pump liquid chambers 44 in the second lifting plate 21, and pistons 42 are provided in the pump liquid chambers 44. The pump liquid chambers 44 are connected to the corresponding negative pressure chambers 26 through connecting pipes. A second electric push rod 20 is fixedly installed at one end of the second lifting plate 21. The telescopic end of the second electric push rod 20 is fixedly connected with a pull rod 43 extending to the multiple pump liquid chambers 44. Multiple pistons 42 are fixed on the pull rod 43. Start the second electric push rod 20, and the pull rod 43 drives the multiple pistons 42 to move, so that negative pressure is generated in the negative pressure chamber 26, and then suction is performed through the connecting hole 27, the suction tube 28 and the suction hole 35. The second electric push rod 20 is reset to squeeze out the liquid.

[0046] A culture chamber 39 is provided on both the front and rear sides of the detection platform 1. A liftable loading plate 40 is installed in the culture chamber 39. Three groups of placement grooves 41 are provided on the loading plate 40. The number of each group of placement grooves 41 is consistent with the number of multiple egg grooves 5 on one side. A cleaning groove 38 is also radially provided on the table top of the detection platform 1. After each pumping process, the drill rod 19 can be transferred to the cleaning groove 38 for cleaning. A cylinder 45 is installed at the bottom of the culture chamber 39. The telescopic end of the cylinder 45 is fixedly connected with a bottom plate 46. The bottom plate 46 is fixed to the loading plate 40 by a rubber pad 14. A vibration motor 47 is installed on the bottom plate of the loading plate 40. A chamber door 37 is installed at the chamber mouth of the culture chamber 39. After the culture of the enrichment solution and the selective enrichment solution, the vibration motor 47 can be started to drive the loading plate 40 to vibrate, so as to achieve mixing before taking the liquid.

[0047] The present invention also provides a method for detecting salmonella on the surface of eggs, comprising the following specific steps:

[0048] S1, selecting a plurality of eggs with dirty surfaces and placing them in a plurality of egg slots 5 on the left, and selecting a plurality of eggs with clean surfaces and placing them in a plurality of egg slots 5 on the right, and fixing them by negative pressure adsorption;

[0049] S2, start the first electric push rod 9 on the left side to drive the first lifting plate 16 to move downward, and the multiple pairs of cotton swabs 18 are pressed against the two sides of the corresponding dirty eggs under the action of the first spring 23, and the multiple turntables 17 are synchronously driven to rotate through the driving mechanism, thereby driving the multiple pairs of cotton swabs 18 to rotate around the surface of the egg for multiple circles to complete the sampling of surface dirt;

[0050] S3, start the first electric push rod 9 on the right side to drive the second lifting plate 21 to move downward, and then drive the multiple drill rods 19 to rotate through the driving mechanism, drill into multiple clean eggs from the top, and then energize the second spring 30 through the controller to make it contract and squeeze the elastic sheet 34, so that it expands and stirs inside the egg, mixes the egg yolk and egg liquid, and then samples are taken through the pumping mechanism;

[0051] S4, pour the prepared enrichment solution into a culture dish and place it in a group of culture tanks 41, the rotating arm 10 cooperates with the lifting to insert the sampled cotton swab 18 and the drill rod 19 into the enrichment solution on the corresponding side, the dirty sample on the cotton swab 18 directly enters the enrichment solution, the egg liquid in the drill rod 19 is squeezed into the corresponding enrichment solution through the pump mechanism, and the carrier plate 40 is lowered into the culture chamber 39 for culture;

[0052] S5, pour the prepared selective enrichment solution into a culture dish and place it in a group of culture tanks 41, use the drill rod 19 and the pump mechanism to extract the enrichment solution in S4 and squeeze it into the selective enrichment solution for culture;

[0053] S6, pour the prepared culture medium into a culture dish and place it in a set of culture tanks 41, use the drill rod 19 and the pump mechanism to extract the selective enrichment solution in S5 and squeeze it onto the culture medium, the drill rod 19 can rotate to achieve the streaking function, and enter the culture chamber 39 for culture;

[0054] S7. Take out the culture medium in S6, and observe the morphology and color of the bacteria in the culture medium under a microscope to determine whether Salmonella is present.

[0055] The multiple egg troughs 5 on the left side can be used to place multiple eggs with dirty surfaces, and the multiple egg troughs 5 on the right side are used to place multiple eggs with clean surfaces. The sealing gaskets 6 can resist the outer periphery of the eggs, so that the egg troughs 5 are in a closed space. The negative pressure pump 2 is started, and the air in the egg troughs 5 can be synchronously sucked through the suction pipe 3 and the multiple negative pressure holes 4, thereby adsorbing the eggs, which is convenient for the subsequent surface and internal sampling process.

[0056] The first electric push rod 9 on the left is started to drive the first lifting plate 16 to move downward. Under the action of the first spring 23, multiple pairs of cotton swabs 18 contact the two sides of the corresponding dirty eggs. Through the driving mechanism and the telescopic shaft 15, multiple turntables 17 are synchronously driven to rotate, thereby driving multiple pairs of cotton swabs 18 to rotate around the surface of the egg for multiple circles to complete the sampling of surface dirt. The first electric push rod 9 on the right is started to drive the second lifting plate 21 to move downward, and then the multiple drill rods 19 are driven to rotate through the driving mechanism and the telescopic shaft 15 to drill into multiple clean eggs from the top. Then, the second spring 30 is energized through the controller to contract and squeeze the elastic sheet 34, so that it expands and stirs inside the egg to mix the yolk and egg liquid. Then, sampling is carried out through the pump mechanism, and the second electric push rod 20 is started. The multiple pistons 42 are driven to move through the pull rod 43 to generate negative pressure in the negative pressure chamber 26, and then suction is carried out through the connecting hole 27, the suction tube 28 and the suction hole 35. The second electric push rod 20 is reset to squeeze out the liquid, and then the suction and extrusion process of the egg liquid, the enrichment liquid and the selective enrichment liquid can be carried out.

[0057] The configured enrichment solution, selective enrichment solution and culture medium are poured into multiple culture dishes respectively, and then placed in the corresponding multiple groups of placement grooves 41 respectively. The rotating arm 10 can be lifted and lowered to insert the sampled cotton swab 18 and the drill rod 19 into the enrichment solution on the corresponding side. The dirty sample on the cotton swab 18 directly enters the enrichment solution, and the egg liquid in the drill rod 19 is squeezed into the corresponding enrichment solution through the pump mechanism. After cultivation, the enrichment solution is extracted by the drill rod 19 and the pump mechanism and squeezed into the selective enrichment solution for cultivation. The selective enrichment solution is extracted by the drill rod 19 and the pump mechanism and squeezed onto the culture medium. The drill rod 19 can realize the streaking function by rotating, and the culture chamber 39 can realize constant temperature cultivation. After the enrichment solution and the selective enrichment solution are cultivated, the vibration motor 47 can be started to drive the carrier plate 40 to vibrate, so as to achieve mixing before taking the liquid.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sampling device for detecting salmonella on egg surface, comprising a detection platform (1), characterized in that: A plurality of egg troughs (5) are provided on the left and right side surfaces of the detection platform (1), and the plurality of egg troughs (5) on both sides are located on the same straight line. The bottoms of the plurality of egg troughs (5) are provided with negative pressure suction holes (4). A rotating column (7) is rotatably connected at the center of the detection platform (1), and a rotating arm (10) is fixedly connected to the top of the rotating column (7). A first lifting plate (16) and a second lifting plate (21) that can be lifted and lowered are respectively installed at the bottoms of the left and right sides of the rotating arm (10). The tops of the first lifting plate (16) and the second lifting plate (21) are rotatably connected to a plurality of telescopic shafts (15). A driving mechanism that can synchronously drive the plurality of telescopic shafts (15) to rotate is installed in the rotating arm (10). A plurality of turntables (17) that are connected one by one with the plurality of telescopic shafts (15) on the left side are installed at the bottom of the first lifting plate (16). The plurality of turntables (17) are connected to the plurality of telescopic shafts (15) on the left side. The egg slots (5) correspond one to one, and a pair of cotton swabs (18) are elastically inserted at the bottom of the plurality of turntables (17). The bottom of the second lifting plate (21) is equipped with a plurality of drill rods (19) that are connected one to one with the plurality of telescopic shafts (15) on the right side. Expandable elastic sheets (34) are embedded on both sides of the drill rods (19). Both sides of the drill bit of the drill rods (19) are provided with suction holes (35). The second lifting plate (21) is equipped with a plurality of pumping mechanisms connected to the suction holes (35) of the corresponding drill rods (19). The front and rear sides of the detection platform (1) are provided with culture chambers (39). A liftable loading plate (40) is installed in the culture chambers (39). Three groups of placement slots (41) are provided on the loading plate (40). The number of the placement slots (41) in each group is consistent with the number of the plurality of egg slots (5) on one side. A cleaning slot (38) is also radially provided on the surface of the detection platform (1).

2. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: A negative pressure pump (2) is installed on the side wall of the detection table (1); the suction end of the negative pressure pump (2) is connected to a suction pipe (3) extending into the detection table (1); a plurality of negative pressure holes (4) are connected to the suction pipe (3); and a sealing gasket (6) is installed at the notch of the egg trough (5).

3. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: First electric push rods (9) are fixedly mounted on the tops of both sides of the rotating arm (10), and the telescopic ends of the two first electric push rods (9) are fixedly connected to the first lifting plate (16) and the second lifting plate (21), respectively.

4. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: The driving mechanism comprises a transmission chamber (11) arranged in a rotating arm (10), a driving shaft (12) being rotatably connected in the transmission chamber (11), the telescopic ends of the plurality of telescopic shafts (15) all extending into the transmission chamber (11), the driving shaft (12) and the extended ends of the plurality of telescopic shafts (15) being transmission-connected via a bevel gear pair (13), and a motor (8) capable of driving the driving shaft (12) to rotate is mounted at one end of the rotating arm (10).

5. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: The bottom of both sides of the rotating disk (17) are provided with a slide groove (22), and a slider (24) is elastically connected to the slide groove (22) via a first spring (23). The bottom of the slider (24) is provided with a socket for inserting the wooden handle end of the cotton swab (18), and a rubber sleeve (25) is installed in the socket to resist the wooden handle end.

6. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: A pair of receiving grooves (33) and a pair of vertical grooves (29) are provided on both sides of the drill rod (19); the lower end of the elastic sheet (34) is fixedly connected to the inner bottom of the corresponding receiving groove (33); the upper end of the elastic sheet (34) is fixedly connected to a stop block (32); the stop block (32) is slidably connected in the corresponding receiving groove (33); a stop rod (31) is elastically connected in the vertical groove (29) via a second spring (30); the lower end of the stop rod (31) extends into the corresponding side receiving groove (33) and is fixedly connected to the stop block (32); the top end of the drill rod (19) is sleeved with an electric coil electrically connected to the second spring (30); and the top of the second lifting plate (21) is provided with a brush (36) in contact with the electric coil and connected to an external controller.

7. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: The pump mechanism comprises a plurality of negative pressure chambers (26) arranged in the second lifting plate (21); the upper end of the drill rod (19) passes through the negative pressure chamber (26) and is fixedly connected to the corresponding telescopic shaft (15); a rotation seal is arranged at the connection between the drill rod (19) and the negative pressure chamber (26); a liquid suction pipe (28) extending vertically and connected to a pair of liquid suction holes (35) is arranged in the drill rod (19); the upper end of the liquid suction pipe (28) is connected to a connecting hole (27) connected to the negative pressure chamber (26); and a suction assembly is also installed in the second lifting plate (21).

8. The egg surface Salmonella detection sampling device according to claim 7, characterized in that: The suction assembly includes a plurality of pump fluid chambers (44) arranged in a second lifting plate (21), a piston (42) being provided in the pump fluid chamber (44), the pump fluid chamber (44) being connected to the corresponding negative pressure chamber (26) via a connecting pipe, a second electric push rod (20) being fixedly mounted on one end of the second lifting plate (21), a telescopic end of the second electric push rod (20) being fixedly connected to a pull rod (43) extending to the plurality of pump fluid chambers (44), and the plurality of pistons (42) being fixed on the pull rod (43).

9. The egg surface Salmonella detection sampling device according to claim 1, characterized in that: A cylinder (45) is installed at the bottom of the culture chamber (39), and the telescopic end of the cylinder (45) is fixedly connected to a bottom plate (46). The bottom plate (46) is fixed to the carrier plate (40) via a rubber pad (14). A vibration motor (47) is installed on the bottom plate of the carrier plate (40). A door (37) is installed at the opening of the culture chamber (39).

10. A detection method based on the egg surface Salmonella detection sampling device according to any one of claims 1 to 9, characterized in that: The specific steps include: S1, selecting a plurality of eggs with dirty surfaces and placing them in a plurality of egg slots (5) on the left side, and selecting a plurality of eggs with clean surfaces and placing them in a plurality of egg slots (5) on the right side, and fixing them by negative pressure adsorption; S2, starting the first electric push rod (9) on the left side to drive the first lifting plate (16) to move downward, and the plurality of pairs of cotton swabs (18) are pressed against the two sides of the corresponding dirty eggs under the action of the first spring (23), and the plurality of turntables (17) are synchronously driven to rotate through the driving mechanism, thereby driving the plurality of pairs of cotton swabs (18) to rotate around the surface of the egg for multiple circles, thereby completing the sampling of the surface dirt; S3, starting the first electric push rod (9) on the right side to drive the second lifting plate (21) to move downward, and then driving the plurality of drill rods (19) to rotate through the driving mechanism, drilling into a plurality of clean eggs from the top, and then energizing the second spring (30) through the controller to cause it to contract and squeeze the elastic sheet (34), causing it to expand and stir inside the egg, so as to mix the egg yolk and the egg liquid, and then sampling through the pumping mechanism; S4, pour the prepared enrichment solution into a culture dish and place it in a set of culture tanks (41), the rotating arm (10) cooperates with the lifting to insert the sampled cotton swab (18) and the drill rod (19) into the enrichment solution on the corresponding side, the dirty sample on the cotton swab (18) directly enters the enrichment solution, the egg liquid in the drill rod (19) is squeezed into the corresponding enrichment solution through the pump mechanism, and the carrier plate (40) is lowered into the culture chamber (39) for culture; S5, pouring the prepared selective enrichment solution into a culture dish and placing it in a set of culture tanks (41), using a drill rod (19) and a pumping mechanism to extract the enrichment solution in S4 and squeeze it into the selective enrichment solution for culture; S6, pour the prepared culture medium into a culture dish and place it in a set of culture tanks (41), use the drill rod (19) and the pump mechanism to extract the selective bacterial enrichment liquid in S5 and squeeze it onto the culture medium, the drill rod (19) can be rotated to achieve a streaking function, and enter the culture chamber (39) for culture; S7. Take out the culture medium in S6, and observe the morphology and color of the bacteria in the culture medium under a microscope to determine whether Salmonella is present.

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