Stem cell exosome high-activity freeze-drying formula and freeze-drying process thereof

By adding sodium alginate, sericin mixture, mannitol and buckwheat extract to the stem cell exosome lyophilized formula, and using prefreeze treatment and vacuum freeze-drying technology, the low exosome resuscitation rate in the existing technology was solved. Problem, the biological activity and stability of the lyophilized formula has been significantly improved and is suitable for a variety of medical applications.

CN119073301AInactive Publication Date: 2024-12-06JINGNENG SCI & TECH (BEIJING) PHARM TECH GRP CO LTD
0 Cites 0 Cited by

Patent Information

Application Number
CN202411187981.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-08-28
Publication Date
2024-12-06
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The exosome resuscitation rate of existing stem cell exosome lyophilized formulas is low, resulting in a decrease in biological activity and affecting tissue repair and therapeutic effects.

Method used

Using a high-active lyophilization formula for stem cell exosomes, including stem cell exosomes, sodium alginate, sericin mixture, mannitol, buckwheat extract and beta-mercaptoethanol, through specific lyophilization processes and treatment steps, including pre- Freeze treatment and vacuum freeze-drying to protect exosome structure and biological activity.

Benefits of technology

It significantly improves the resuscitation rate and biological stability of stem cell exosome lyophilized formula, maintains the high activity of exosomes, and is suitable for regenerative medicine, disease diagnosis and treatment and other fields.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a stem cell exosome high-activity freeze-drying formula and a freeze-drying process thereof, and relates to the technical field of biology, the stem cell exosome high-activity freeze-drying formula is prepared from the following components: 90-100 parts of stem cell exosome, 1-5 parts of sodium alginate, 3-6 parts of a sericin protein mixture, 5-15 parts of mannitol, 0.3-0.6 part of a tartary buckwheat extract, and beta-mercaptoethanol; the stem cell exosome freeze-drying formula prepared by the invention has higher activity, and can maintain higher recovery rate after hydration, so that the biological stability of the stem cell exosome freeze-drying formula is greatly improved. According to the method, moisture is directly sublimated from a solid state to a gaseous state under a vacuum condition, so that the damage to an exosome structure caused by ice crystal formation is avoided, the low-temperature and low-pressure environment can effectively reduce the oxidation and degradation reaction of the exosome, and the biological activity of the obtained stem cell exosome freeze-drying formula is kept.
Need to check novelty before this filing date? Find Prior Art