Anti-allergy soothing non-allergy surface layer material, preparation method thereof and sanitary towel prepared from anti-allergy soothing non-allergy surface layer material

Abstract

The invention provides an anti-allergy soothing non-allergy surface layer material, a preparation method thereof and a sanitary towel prepared from the anti-allergy soothing non-allergy surface layer material, and belongs to the technical field of sanitary towels. Nostoc commune and nostoc sphaeroids kutz are subjected to enzymolysis separation to prepare algae antibacterial peptide, the algae antibacterial peptide is connected with cordycepin, chelated with Cu ions and added into a chitosan solution together with probiotic microspheres and a purslane flavone extract in a stirring mode, temperature-sensitive hydrogel is prepared and evenly sprayed on cotton cloth, and the anti-allergy soothing non-sensitive surface layer material is prepared. The prepared anti-allergy soothing non-allergy surface layer material does not contain chemical allergens, the allergy risk is inhibited from the source, the whole production link is produced in a sterile and dust-free purified environment, no sensitization is achieved, the material is more skin-friendly, and meanwhile the material has multiple biological functions of resisting bacteria and inflammation, regulating and controlling ecological balance of private parts and the like, so that skin itch is relieved, skin is relieved, allergy is resisted, and the anti-allergy soothing non-allergy surface layer material has a good application prospect. The health-care product has the effects of protecting skin barrier, resisting inflammation and the like, also can reduce colpitis and the like, is good in skin friendliness, protects the private health of women in a menstrual period, and is more comfortable to use.

Description

Technical Field

[0001] The invention relates to the technical field of sanitary napkins, and in particular to an anti-allergic and soothing non-allergic surface layer material, a preparation method thereof, and the prepared sanitary napkin. Background Art

[0002] Sanitary napkins are sanitary products used by women during menstruation. The main materials are cotton, non-woven fabrics, pulp or polymers and polymer composite paper formed by the above materials. The side design is mainly used to prevent side leakage. From the inside to the outside, it is divided into three layers: surface layer, absorbent core, and bottom layer. When using, tear off the paper tape in the middle first, stick the adhesive side to the middle of the underwear first, and stick the two "ears" to the narrowest part of the crotch. Other common menstrual products include tampons, menstrual cups, panty liners, etc. With the development of science and technology and the gradual improvement of people's quality of life, the types of sanitary napkins are gradually enriched.

[0003] For example, in the patent with publication number CN116492145A, a sanitary napkin with antibacterial and deodorizing properties and its preparation process are disclosed. In the sanitary napkin, viscose fibers and bamboo fibers of different finenesses are used to design a double-layer composite spunlace non-woven fabric, so as to enable the liquid to move from the viscose fiber side to the bamboo fiber side, thereby achieving unidirectional moisture conduction, preventing backseepage, and having excellent antibacterial properties.

[0004] For example, the patent with announcement number CN115414185B discloses a highly dry sanitary napkin and its production process, which includes a surface layer, a menthol sustained-release agent layer, an absorbent core layer and a bottom film from top to bottom, wherein the surface layer is a unidirectional water-permeable spinning film, which is made of a polyurethane fiber film and a pure cotton non-woven fabric through an electrostatic spinning process, and the polyurethane fiber film is located on the pure cotton non-woven fabric. The sanitary napkin has strong water absorption, high air permeability, a dry surface, and is comfortable to touch the skin without irritation or dampness.

[0005] From the above examples, we can also find that the existing improvements on sanitary napkins are mainly focused on improving the water absorption capacity of sanitary napkins and keeping the surface dry. However, in actual use, it can be found that in addition to menstrual blood, there are also blood clots and endometrial tissue discharged during the physiological period. Therefore, even if the sanitary napkin has good water absorption performance, these blocky substances will still remain on the surface of the sanitary napkin, causing bacterial growth, causing skin itching, vaginitis and other problems. Some sanitary napkin materials are even prone to skin allergic reactions, causing skin itching and other problems. Summary of the invention

[0006] The purpose of the present invention is to propose an anti-allergic and soothing non-allergic surface material and a preparation method thereof and a sanitary napkin prepared therefrom, which has multiple biological functions such as antibacterial and anti-inflammatory, antioxidant, and immune regulation, thereby alleviating skin itching, soothing the skin, anti-allergic, protecting the skin barrier, and anti-inflammatory effects. It can also reduce the occurrence of vaginitis, has good skin-friendliness, protects the private health of women during menstruation, and is more comfortable to use.

[0007] The technical solution of the present invention is achieved in this way:

[0008] The invention provides a method for preparing an anti-allergic and soothing non-allergic surface layer material. The method comprises the following steps: enzymatically separating Auricularia auricula and Miscanthus puerariae to obtain an algae antimicrobial peptide, connecting the algae antimicrobial peptide with cordycepin, chelating Cu ions, adding the algae antimicrobial peptide with probiotic microspheres and a purslane flavonoid extract into a chitosan solution, stirring the solution, and obtaining a thermosensitive hydrogel. The thermosensitive hydrogel is evenly sprayed on cotton cloth to obtain the anti-allergic and soothing non-allergic surface layer material.

[0009] As a further improvement of the present invention, the following steps are included:

[0010] S1. Preparation of algae antimicrobial peptides: Wash the ground fungus and the kudzu rice, dry them, and crush them to obtain algae powder, add enzymes and cellulases, stir and hydrolyze them, add the seed liquid of Lactobacillus plantarum and Bifidobacterium lactis, ferment them with enzymes, filter, wash the solids, collect the bacterial liquid, and retain the solid residue; the filtrate is subjected to ammonium sulfate graded precipitation and freeze-dried to obtain algae antimicrobial peptides;

[0011] S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: add cordycepic acid to water, add NHS and EDC, activate the reaction, add algae antimicrobial peptide, stir the reaction, dialyze, and freeze-dry to obtain algae antimicrobial peptide-cordycepic acid cages;

[0012] S3. Preparation of Cu-chelated algae antimicrobial peptide-cordycepic acid cage: Dissolve copper salt in water, add algae antimicrobial peptide-cordycepic acid cage, stir to react, dialyze, freeze-dry, and obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage;

[0013] S4. Preparation of probiotic microspheres: adding sodium alginate and Tween to the bacterial solution, adding dropwise to the fish oil, emulsifying, adding dropwise calcium chloride solution, solidifying at room temperature, centrifuging, washing, and drying to obtain probiotic microspheres;

[0014] S5. Preparation of flavonoid extract from purslane: The purslane was washed, dried, chopped, added to water, added with sad enzyme and cellulase, stirred for enzymolysis, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin to obtain a flavonoid extract from purslane;

[0015] S6. Preparation of thermosensitive hydrogel: Dissolve chitosan in acid solution, add Cu-chelated algae antimicrobial peptide-cordycepic acid cage, probiotic microspheres and purslane flavonoid extract, stir and mix well to prepare thermosensitive hydrogel;

[0016] S7. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on cotton cloth to prepare the anti-allergic and soothing non-allergic surface material.

[0017] As a further improvement of the present invention, the mass ratio of the ground fungus and the kudzu vine in step S1 is 4-6:5-7, the mass ratio of the algae powder, the enzyme and the cellulase is 10:0.2-0.5:1-2, the temperature of the enzymolysis is 35-45 ° C, the time is 1-2h, the bacterial content of the plant lactobacillus and the lactobacillus bifidum seed liquid is 10 8 -10 9 cfu / mL, the enzyme-assisted fermentation culture conditions are 36-38°C, 100-200r / min, under anaerobic conditions, the enzyme-assisted fermentation culture is 24-36h, and the bacterial liquid contains 10 9 -10 10 cfu / mL.

[0018] As a further improvement of the present invention, the mass ratio of cordycepic acid, NHS, EDC and algae antimicrobial peptide in step S2 is 10:5-7:3-5:15-18, the temperature of the activation reaction is 0-4°C, the time is 20-30min, and the stirring reaction time is 12-15h.

[0019] As a further improvement of the present invention, the copper salt in step S3 is selected from at least one of copper chloride, copper sulfate, and copper nitrate, the mass ratio of the copper salt to the algae antimicrobial peptide-cordycepic acid cage is 0.5-1:10, and the stirring reaction time is 20-40 minutes.

[0020] As a further improvement of the present invention, the mass ratio of the bacterial solution, sodium alginate and Tween in step S4 is 100:12-15:1-2, and the Tween is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

[0021] As a further improvement of the present invention, the mass ratio of purslane, enzyme and cellulase in step S5 is 10:0.5-1:1-2, the temperature of the enzymatic hydrolysis is 35-45°C, the time is 1-2h, the macroporous resin is selected from at least one of D-101, AB-8, and DA-201, and the eluent used for purification is 45-65wt% ethanol solution.

[0022] As a further improvement of the present invention, the acid solution in step S6 is a 2-4wt% acetic acid or lactic acid solution, the mass ratio of the chitosan, the Cu-chelated algae antimicrobial peptide-cordycepic acid cage, the probiotic microspheres and the purslane flavonoid extract is 20-30:5-7:2-3:3-4; the spraying amount of the thermosensitive hydrogel on the cotton cloth in step S7 is 3-5mL / cm2 .

[0023] The present invention further protects an anti-allergic and soothing non-allergic surface material prepared by the above-mentioned preparation method.

[0024] The present invention further protects a sanitary napkin made of the above-mentioned anti-allergic and soothing non-allergic surface layer material.

[0025] The present invention has the following beneficial effects:

[0026] The present invention uses laccase and cellulase to perform preliminary enzymolysis on purslane. Laccase is a kind of oxidoreductase, which is composed of protein and Cu 2+ It is composed of 2,4-dimethoxy-2-nitropropene, 1,2-dimethoxy-1 ...

[0027] The fungus and radix puerariae are algae rich in protein, and they themselves contain algae protein with high antibacterial activity. At the same time, after fermentation, the protein can generate algae antimicrobial peptides with high antibacterial and antifungal activity under the fermentation action of plant lactobacillus and bifidobacterium lactis, and after coupling with cordycepic acid, a composite cage structure is formed, which greatly improves the antibacterial, anti-inflammatory and antioxidant effects of the product, and also has the effects of soothing the skin and anti-allergic. At the same time, the loading amount of Cu ions can be increased, and the loading of Cu ions improves the antibacterial performance of the product, and can be combined with microbial deoxyribonucleic acid (DNA) and sulfhydryl groups of metabolic enzymes in the electron transport chain of the organism to form a stable coordination complex, which can effectively kill pathogenic microorganisms and effectively prevent them from developing drug resistance. At the same time, the protein can also be used as a cross-linking agent to promote the cross-linking of chitosan, is in a solution state at room temperature, and forms a gel state at body temperature, so that the surface layer prepared by the invention has the characteristic of being skin-friendly.

[0028] Probiotic microspheres are also added to the thermosensitive hydrogel of the present invention, which, on the one hand, improve the survival rate of probiotics and improve their stability; on the other hand, the probiotic microspheres are broken during use, and the probiotics are released and enter the vagina, regulating the vaginal flora, reducing the occurrence of odor and vaginitis, etc., protecting the private health of women during menstruation, and being more beneficial to women's health.

[0029] The anti-allergic and soothing non-allergenic surface material prepared by the present invention does not contain chemical allergens, thus suppressing the risk of allergies at the source, and the entire production chain is produced in a sterile, dust-free and purified environment, is non-allergenic and more skin-friendly, and simultaneously has multiple biological functions such as antibacterial and anti-inflammatory, and regulation of the ecological balance of the private parts, thereby alleviating skin itching, soothing the skin, anti-allergic, protecting the skin barrier, and anti-inflammatory effects, and can also reduce the occurrence of vaginitis, etc. It has good skin-friendliness, protects the private health of women during the menstrual period, and is more comfortable to use. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Lactobacillus plantarum, 10 billion cfu / g, Bifidobacterium lactis, 10 billion cfu / g, were purchased from Shandong Zhongke Jiayi Bioengineering Technology Co., Ltd. Preparation of Lactobacillus plantarum and Bifidobacterium lactis seed solution: The strains were inoculated into Gao's medium, activated and cultured at 37°C, 100 r / min for 18-24 h, and a culture medium with a bacterial content of 10 8 -10 9 cfu / mL of bacterial seed solution.

[0032] NHS (N-hydroxysuccinimide) and EDC (1-ethyl-(3-dimethylaminopropyl)carbodiimide).

[0033] Cellulase, 10,000 U / g, was purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.

[0034] Example 1

[0035] This embodiment provides a method for preparing an anti-allergic and soothing non-allergic surface layer material, comprising the following steps:

[0036] S1. Preparation of algae antimicrobial peptides: 40 g of ground fungus and 50 g of kudzu vine were washed, dried, and crushed to obtain algae powder. 10 g of algae powder was added with 0.2 g of enzyme and 1 g of cellulase, stirred, heated to 35 ° C, and enzymolyzed for 1 h. The seed liquid of Lactobacillus plantarum and Bifidobacterium lactis was added, and the enzyme-assisted fermentation culture was carried out for 24 h at 36 ° C and 100 r / min under anaerobic conditions. The liquid was filtered, the solid was washed, and the bacterial liquid was collected. The bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was subjected to ammonium sulfate graded precipitation and freeze-dried to obtain the algae antimicrobial peptide;

[0037] S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: 10 g of cordycepic acid was added to 500 mL of water, 5 g of NHS and 3 g of EDC were added, activated at 0°C for 20 min, 15 g of algae antimicrobial peptide was added, stirred for 12 h, dialyzed, and freeze-dried to obtain algae antimicrobial peptide-cordycepic acid cages;

[0038] S3. Preparation of Cu-chelated algae antimicrobial peptide-cordycepic acid cage: 0.5 g of copper nitrate was dissolved in 200 mL of water, 10 g of algae antimicrobial peptide-cordycepic acid cage was added, stirred for 20 min, dialyzed, and freeze-dried to obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage;

[0039] S4. Preparation of probiotic nanospheres: 12 g of sodium alginate and 1 g of Tween-20 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 5000 r / min for 15 min, 20 mL of 2 wt% calcium chloride solution was added dropwise, solidified at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic nanospheres;

[0040] S5. Preparation of flavonoid extract from purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, 0.5 g of enzyme and 1 g of cellulase were added, stirred, heated to 35 ° C, enzymolysis for 1 h, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin AB-8, and the eluate of 45-65 wt% ethanol solution was collected and dried to obtain a flavonoid extract from purslane;

[0041] S6. Preparation of thermosensitive hydrogel: 20 g of chitosan was dissolved in 1000 mL of 2 wt% acetic acid solution, 5 g of Cu-chelated algae antimicrobial peptide-cordycepic acid cage, 2 g of nano-probiotic microspheres and 3 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to obtain a thermosensitive hydrogel;

[0042] S7. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on the cotton cloth at a spraying amount of 3 mL / cm 2 , dried to produce an anti-allergic and soothing non-allergenic surface material.

[0043] Example 2

[0044] This embodiment provides a method for preparing an anti-allergic and soothing non-allergic surface layer material, comprising the following steps:

[0045] S1. Preparation of algae antimicrobial peptides: 60 g of ground fungus and 70 g of kudzu vine were washed, dried, and crushed to obtain algae powder. 10 g of algae powder was added with 0.5 g of enzyme and 2 g of cellulase, stirred, heated to 45 ° C, and enzymolyzed for 2 h. The seed solution of Lactobacillus plantarum and Bifidobacterium lactis was added, and the enzyme-assisted fermentation culture was carried out for 36 h at 38 ° C and 200 r / min under anaerobic conditions. The solution was filtered, the solid was washed, and the bacterial solution was collected. The bacterial count was 109 -10 10 cfu / mL, the solid residue was retained; the filtrate was subjected to ammonium sulfate graded precipitation and freeze-dried to obtain the algae antimicrobial peptide;

[0046] S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: 10 g of cordycepic acid was added to 500 mL of water, 7 g of NHS and 5 g of EDC were added, and the reaction was activated at 4°C for 30 min, 18 g of algae antimicrobial peptide was added, and the reaction was stirred for 15 h, dialyzed, and freeze-dried to obtain algae antimicrobial peptide-cordycepic acid cages;

[0047] S3. Preparation of Cu-chelated algae antimicrobial peptide-cordycepic acid cage: 1 g of copper sulfate was dissolved in 200 mL of water, 10 g of algae antimicrobial peptide-cordycepic acid cage was added, stirred for 40 min, dialyzed, and freeze-dried to obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage;

[0048] S4. Preparation of probiotic nanospheres: 15 g of sodium alginate and 2 g of Tween-60 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 5000 r / min for 15 min, 20 mL of 2 wt% calcium chloride solution was added dropwise, solidified at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic nanospheres;

[0049] S5. Preparation of flavonoid extract from purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, 1 g of enzyme and 2 g of cellulase were added, stirred, heated to 45 ° C, enzymolysis for 2 h, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin AB-8, and the eluate of 65 wt% ethanol solution was collected and dried to obtain a flavonoid extract from purslane;

[0050] S6. Preparation of thermosensitive hydrogel: 30 g of chitosan was dissolved in 1000 mL of 4 wt% lactic acid solution, 7 g of Cu-chelated algae antimicrobial peptide-cordycepic acid cage, 3 g of nano-probiotic microspheres and 4 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to obtain a thermosensitive hydrogel;

[0051] S7. Preparation of anti-allergic and soothing non-allergic surface material: Spray the thermosensitive hydrogel evenly on the cotton cloth at a spraying amount of 5 mL / cm 2 , dried to produce an anti-allergic and soothing non-allergenic surface material.

[0052] Example 3

[0053] This embodiment provides a method for preparing an anti-allergic and soothing non-allergic surface layer material, comprising the following steps:

[0054] S1. Preparation of algae antimicrobial peptides: 50g of ground fungus and 60g of kudzu vine were washed, dried, and crushed to obtain algae powder. 10g of algae powder was added with 0.35g of enzyme and 1.5g of cellulase, stirred, heated to 40°C, and enzymolyzed for 1.5h. The seed liquid of Lactobacillus plantarum and Bifidobacterium lactis was added, and the culture was fermented with enzyme for 30h at 37°C and 150r / min under anaerobic conditions. The culture was filtered, the solid was washed, and the bacterial liquid was collected. The bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was subjected to ammonium sulfate graded precipitation and freeze-dried to obtain the algae antimicrobial peptide;

[0055] S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: 10 g of cordycepic acid was added to 500 mL of water, 6 g of NHS and 4 g of EDC were added, and the reaction was activated at 2°C for 25 min, 17 g of algae antimicrobial peptide was added, and the reaction was stirred for 13 h, dialyzed, and freeze-dried to obtain algae antimicrobial peptide-cordycepic acid cages;

[0056] S3. Preparation of Cu-chelated algae antimicrobial peptide-cordycepic acid cage: 0.6 g of copper chloride was dissolved in 200 mL of water, 10 g of algae antimicrobial peptide-cordycepic acid cage was added, stirred for 30 min, dialyzed, and freeze-dried to obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage;

[0057] S4. Preparation of probiotic nanospheres: 13 g of sodium alginate and 1.5 g of Tween-85 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 5000 r / min for 15 min, 20 mL of 2 wt% calcium chloride solution was added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic nanospheres;

[0058] S5. Preparation of flavonoid extract from purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, 0.7 g of enzyme and 1.5 g of cellulase were added, stirred, heated to 40 ° C, enzymolysis for 1.5 h, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin AB-8, and the eluate of 45-65 wt% ethanol solution was collected and dried to obtain a flavonoid extract from purslane;

[0059] S6. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt% acetic acid solution, 6 g of Cu-chelated algae antimicrobial peptide-cordycepic acid cage, 2.5 g of nano-probiotic microspheres and 3.5 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to obtain a thermosensitive hydrogel;

[0060] S7. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on cotton cloth at a spraying amount of 4 mL / cm 2 , dried to produce an anti-allergic and soothing non-allergenic surface material.

[0061] Comparative Example 1

[0062] Compared with Example 3, the difference is that step S2 is not performed.

[0063] The details are as follows:

[0064] S1. Preparation of algae antimicrobial peptides: 50g of ground fungus and 60g of kudzu vine were washed, dried, and crushed to obtain algae powder. 10g of algae powder was added with 0.35g of enzyme and 1.5g of cellulase, stirred, heated to 40°C, and enzymolyzed for 1.5h. The seed liquid of Lactobacillus plantarum and Bifidobacterium lactis was added, and the culture was fermented with enzyme for 30h at 37°C and 150r / min under anaerobic conditions. The culture was filtered, the solid was washed, and the bacterial liquid was collected. The bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was subjected to ammonium sulfate graded precipitation and freeze-dried to obtain the algae antimicrobial peptide;

[0065] S2. Preparation of Cu-chelated algae antimicrobial peptide: 0.6 g of copper chloride was dissolved in 200 mL of water, 10 g of algae antimicrobial peptide-cordycepic acid cage was added, the reaction was stirred for 30 min, dialyzed, and freeze-dried to obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage;

[0066] S3. Preparation of probiotic nanospheres: 13 g of sodium alginate and 1.5 g of Tween-85 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 5000 r / min for 15 min, 20 mL of 2 wt% calcium chloride solution was added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic nanospheres;

[0067] S4. Preparation of flavonoid extract from purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, 0.7 g of enzyme and 1.5 g of cellulase were added, stirred, heated to 40 ° C, enzymolysis for 1.5 h, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin AB-8, and the eluate of 45-65 wt% ethanol solution was collected and dried to obtain a flavonoid extract from purslane;

[0068] S5. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt% acetic acid solution, 6 g of Cu-chelated algae antimicrobial peptide, 2.5 g of nano-probiotic microspheres and 3.5 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to obtain a thermosensitive hydrogel;

[0069] S6. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on the cotton cloth at a spraying amount of 4 mL / cm 2 , dried to produce an anti-allergic and soothing non-allergenic surface material.

[0070] Comparative Example 2

[0071] Compared with Example 3, the difference is that in step S2, the algae antimicrobial peptide and cordycepic acid are simply mixed.

[0072] The details are as follows:

[0073] S2. Preparation of the mixture: 10 g of cordycepic acid and 17 g of algae antimicrobial peptide were mixed for 20 min to prepare a mixture.

[0074] Comparative Example 3

[0075] Compared with Example 3, the difference is that step S3 is not performed.

[0076] The details are as follows:

[0077] S1. Preparation of algae antimicrobial peptides: 50g of ground fungus and 60g of kudzu vine were washed, dried, and crushed to obtain algae powder. 10g of algae powder was added with 0.35g of enzyme and 1.5g of cellulase, stirred, heated to 40°C, and enzymolyzed for 1.5h. The seed liquid of Lactobacillus plantarum and Bifidobacterium lactis was added, and the culture was fermented with enzyme for 30h at 37°C and 150r / min under anaerobic conditions. The culture was filtered, the solid was washed, and the bacterial liquid was collected. The bacterial count was 10 9 -10 10 cfu / mL, the solid residue was retained; the filtrate was subjected to ammonium sulfate graded precipitation and freeze-dried to obtain the algae antimicrobial peptide;

[0078] S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: 10 g of cordycepic acid was added to 500 mL of water, 6 g of NHS and 4 g of EDC were added, and the reaction was activated at 2°C for 25 min, 17 g of algae antimicrobial peptide was added, and the reaction was stirred for 13 h, dialyzed, and freeze-dried to obtain algae antimicrobial peptide-cordycepic acid cages;

[0079] S3. Preparation of probiotic nanospheres: 13 g of sodium alginate and 1.5 g of Tween-85 were added to 100 g of bacterial solution, added dropwise to 500 mL of fish oil, emulsified at 5000 r / min for 15 min, 20 mL of 2 wt% calcium chloride solution was added dropwise, cured at room temperature for 30 min, centrifuged, washed, and dried to obtain probiotic nanospheres;

[0080] S4. Preparation of flavonoid extract from purslane: 10 g of purslane was washed, dried, chopped, added to 500 mL of water, 0.7 g of enzyme and 1.5 g of cellulase were added, stirred, heated to 40 ° C, enzymolysis for 1.5 h, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin AB-8, and the eluate of 45-65 wt% ethanol solution was collected and dried to obtain a flavonoid extract from purslane;

[0081] S5. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt% acetic acid solution, 6 g of algae antimicrobial peptide-cordycepic acid cage, 2.5 g of nano-probiotic microspheres and 3.5 g of purslane flavonoid extract were added, and stirred for 20 min to obtain a thermosensitive hydrogel;

[0082] S6. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on the cotton cloth at a spraying amount of 4 mL / cm 2 , dried to produce an anti-allergic and soothing non-allergenic surface material.

[0083] Comparative Example 4

[0084] Compared with Example 3, the difference is that the Cu-chelating algae antimicrobial peptide-cordycepic acid cage is not added in step S6.

[0085] The details are as follows:

[0086] S6. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt % acetic acid solution, 2.5 g of nano probiotic microspheres and 3.5 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to prepare a thermosensitive hydrogel.

[0087] Comparative Example 5

[0088] Compared with Example 3, the difference is that no nano probiotic microspheres are added in step S6.

[0089] The details are as follows:

[0090] S6. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt% acetic acid solution, 6 g of Cu-chelated algae antimicrobial peptide-cordycepic acid cage and 3.5 g of purslane flavonoid extract were added, and the mixture was stirred for 20 min to prepare thermosensitive hydrogel.

[0091] Comparative Example 6

[0092] Compared with Example 3, the difference is that the purslane flavonoids extract is not added in step S6.

[0093] The details are as follows:

[0094] S6. Preparation of thermosensitive hydrogel: 25 g of chitosan was dissolved in 1000 mL of 3 wt% acetic acid solution, 6 g of Cu-chelated algae antimicrobial peptide-cordycepic acid cage and 2.5 g of nano probiotic microspheres were added, and the mixture was stirred for 20 min to prepare thermosensitive hydrogel.

[0095] Test Example 1

[0096] The anti-allergic and soothing non-allergic surface materials prepared in Examples 1-3 and Comparative Examples 1-6 of the present invention were used to prepare sanitary napkins. 100 mL of physiological saline containing 50 mg / mL hemoglobin was absorbed and volunteers were asked to wear the sanitary napkins. After 2 hours, the volunteers were asked to evaluate the feeling when wearing the sanitary napkins and whether allergic symptoms occurred. The results are shown in Table 1.

[0097] Table 1

[0098] Group Physical evaluation Are you allergic? Example 1 Good skin-friendly feeling, breathable and comfortable no Example 2 Good skin-friendly feeling, breathable and comfortable no Example 3 Good skin-friendly feeling, breathable and comfortable no Comparative Example 1 Good skin-friendly feeling, breathable and comfortable no Comparative Example 2 Good skin-friendly feeling, breathable and comfortable no Comparative Example 3 Poor skin-friendliness, feels sticky no Comparative Example 4 Poor skin-friendliness, feels sticky no Comparative Example 5 Good skin-friendly feeling, breathable and comfortable no Comparative Example 6 Good skin-friendly feeling, breathable and comfortable no

[0099] It can be seen from the above table that the anti-allergic and soothing non-allergic surface materials prepared in Examples 1-3 of the present invention have good body feel, good skin-friendliness, and are breathable and comfortable.

[0100] After the odor judgment is completed, the number of colonies on the sanitary napkin is measured. The surface layer of the sanitary napkin is cut into pieces, the chip is taken out, and then dispersed in 100 mL of deionized water, soaked for 3 hours, filtered, and 1 mL of the sample is cultured at 37°C for 48 hours to determine the total number of colonies. The results are shown in Table 2.

[0101] Table 2

[0102] Group Escherichia coli (cfu / mL) Staphylococcus aureus (cfu / mL) Candida albicans (cfu / mL) Example 1 22 32 94 Example 2 24 35 95 Example 3 20 30 92 Comparative Example 1 73 84 221 Comparative Example 2 56 72 168 Comparative Example 3 95 124 346 Comparative Example 4 189 274 592 Comparative Example 5 102 213 396 Comparative Example 6 144 259 514

[0103] It can be seen from the above table that the anti-allergic and soothing non-allergic surface materials prepared in Examples 1-3 of the present invention have good antibacterial effects.

[0104] Test Example 2

[0105] RAW264.7 cells were added to DMEM high-glucose culture medium containing 10% fetal bovine serum and placed in an incubator (37°C, 5% CO 2 When the cell confluence reaches 80% to 90%, take the cell suspension and adjust the cell density to 7×10 4 1000 μL of cell suspension was added to each well of a 24-well plate and placed in an incubator (37°C, 5% CO 2 ) and cultured at a constant temperature for 24 h. When the cell plating rate in the well plate met the experimental requirements, the model group and the blank group were replaced with new complete culture medium, the positive control group was added with complete culture medium containing 100 μg / mL dexamethasone, and the experimental group was added with culture medium containing sample solution (1 cm 2The anti-allergic and soothing non-allergic surface material prepared in Example 1-3 or Comparative Example 1-6 was soaked in 50 mL of water for 2 hours, and the material was removed to obtain a sample solution), and the cells were pretreated for 2 hours. Except for the blank group, the model group and the test group were added with a complete culture medium containing lipopolysaccharide solution (5 μg / mL) and continued to be incubated at 37°C for 16 hours. The supernatant was collected and centrifuged, and the supernatant was taken for TNF-α content detection according to the instructions of the ELISA kit. Each group of experiments was repeated 3 times and the average value was taken. The TNF-α inhibition rate (%) was calculated. The results are shown in Table 3.

[0106] TNF-α inhibition rate (%) = average TNF-α content in the experimental group / average TNF-α content in the model group × 100%

[0107] Table 3

[0108]

[0109]

[0110] It can be seen from the above table that the anti-allergic and soothing non-allergic surface materials prepared in Examples 1-3 of the present invention have good anti-inflammatory effects.

[0111] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing an anti-allergic and soothing non-allergic surface material, characterized in that: The algae antimicrobial peptides were prepared by enzymatic separation of Auricularia auricula and Pueraria lobata, connected with cordycepin, chelated with Cu ions, mixed with probiotic microspheres and purslane flavonoids extract, and added into chitosan solution with stirring to prepare thermosensitive hydrogel, which was evenly sprayed on cotton cloth to prepare anti-allergic and soothing non-allergic surface material.

2. The preparation method according to claim 1, characterized in that: The following steps are involved: S1. Preparation of algae antimicrobial peptides: Wash the ground fungus and the kudzu rice, dry them, and crush them to obtain algae powder, add enzymes and cellulases, stir and hydrolyze them, add the seed liquid of Lactobacillus plantarum and Bifidobacterium lactis, ferment them with enzymes, filter, wash the solids, collect the bacterial liquid, and retain the solid residue; the filtrate is subjected to ammonium sulfate graded precipitation and freeze-dried to obtain algae antimicrobial peptides; S2. Preparation of algae antimicrobial peptide-cordycepic acid cages: add cordycepic acid to water, add NHS and EDC, activate the reaction, add algae antimicrobial peptide, stir the reaction, dialyze, and freeze-dry to obtain algae antimicrobial peptide-cordycepic acid cages; S3. Preparation of Cu-chelated algae antimicrobial peptide-cordycepic acid cage: Dissolve copper salt in water, add algae antimicrobial peptide-cordycepic acid cage, stir to react, dialyze, freeze-dry, and obtain Cu-chelated algae antimicrobial peptide-cordycepic acid cage; S4. Preparation of probiotic microspheres: adding sodium alginate and Tween to the bacterial solution, adding dropwise to the fish oil, emulsifying, adding dropwise calcium chloride solution, solidifying at room temperature, centrifuging, washing, and drying to obtain probiotic microspheres; S5. Preparation of flavonoid extract from purslane: The purslane was washed, dried, chopped, added to water, added with sad enzyme and cellulase, stirred for enzymolysis, filtered, and the filtrate was dried to obtain an extract, which was purified with a macroporous resin to obtain a flavonoid extract from purslane; S6. Preparation of thermosensitive hydrogel: Dissolve chitosan in acid solution, add Cu-chelated algae antimicrobial peptide-cordycepic acid cage, probiotic microspheres and purslane flavonoid extract, stir and mix well to prepare thermosensitive hydrogel; S7. Preparation of anti-allergic and soothing non-allergic surface material: spray the thermosensitive hydrogel evenly on cotton cloth to prepare the anti-allergic and soothing non-allergic surface material.

3. The preparation method according to claim 2, characterized in that: In step S1, the mass ratio of ground fungus and kudzu vine is 4-6:5-7, the mass ratio of algae powder, enzyme and cellulase is 10:0.2-0.5:1-2, the temperature of the enzymolysis is 35-45 ° C, the time is 1-2h, the bacterial content of the plant lactobacillus and bifidobacterium lactis seed solution is 10 8 -10 9 cfu / mL, the conditions of the enzyme-assisted fermentation culture are 36-38°C, 100-200r / min, and anaerobic conditions, and the enzyme-assisted fermentation culture is 24-36h, and the bacterial liquid has a bacterial content of 10 9 -10 10 cfu / mL.

4. The preparation method according to claim 2, characterized in that: In step S2, the mass ratio of cordycepic acid, NHS, EDC and algae antimicrobial peptide is 10:5-7:3-5:15-18, the temperature of the activation reaction is 0-4°C, the time is 20-30min, and the stirring reaction time is 12-15h.

5. The preparation method according to claim 2, characterized in that: In step S3, the copper salt is selected from at least one of copper chloride, copper sulfate and copper nitrate, the mass ratio of the copper salt to the algae antimicrobial peptide-cordycepic acid cage is 0.5-1:10, and the stirring reaction time is 20-40 minutes.

6. The preparation method according to claim 2, characterized in that: The mass ratio of the bacterial solution, sodium alginate and Tween in step S4 is 100:12-15:1-2, and the Tween is selected from at least one of Tween-20, Tween-40, Tween-60, Tween-80 and Tween-85.

7. The preparation method according to claim 2, characterized in that: In step S5, the mass ratio of purslane, enzyme and cellulase is 10:0.5-1:1-2, the temperature of the enzymolysis is 35-45°C, the time is 1-2h, the macroporous resin is selected from at least one of D-101, AB-8, and DA-201, and the eluent used for purification is 45-65wt% ethanol solution.

8. The preparation method according to claim 2, characterized in that: The acid solution in step S6 is 2-4wt% acetic acid or lactic acid solution, the mass ratio of chitosan, Cu-chelated algae antimicrobial peptide-cordycepic acid cage, probiotic microspheres and purslane flavonoid extract is 20-30:5-7:2-3:3-4; the spraying amount of the thermosensitive hydrogel on the cotton cloth in step S7 is 3-5mL / cm 2 .

9. An anti-allergic and soothing non-allergenic surface material prepared by the preparation method according to any one of claims 1 to 8.

10. A sanitary napkin made of the anti-allergic and soothing non-allergic surface material as claimed in claim 9.

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