Detection equipment and detection method for pediatric Chinese magnoliavine fruit and cassia bark antipyretic granules

Abstract

The invention discloses detection equipment and a detection method for pediatric Chaigui antipyretic granules. The detection equipment comprises a test sample preparation device for preparing a test sample solution and a control sample preparation device for preparing a control solution, the test sample preparation device comprises a test sample box, a bracket arranged in the test sample box in a lifting manner, a filter screen movably arranged on the bracket, a sampling inspection mechanism for sampling inspection of solids and a dissolving mechanism for rapidly dissolving baicalin; the sampling inspection mechanism comprises a sampling inspection box arranged on the support, a reciprocating assembly used for achieving reciprocating overturning of the filter screen, a drying assembly used for rapidly drying solid particles and a weighing assembly used for weighing the solid particles. The sampling inspection box is provided with a sampling inspection opening, solid particles enter the sampling inspection opening in the overturning process of the filter screen, and the sampling inspection box is provided with a control assembly at the sampling inspection opening. The invention has the effects of good durability, low baseline noise, high sensitivity and good peak shape; other complex pretreatment is not needed in the detection process, and unnecessary interference can be avoided.

Description

Technical Field

[0001] The present application relates to the field of detection of Bupleurum chinense antipyretic granules for children, and in particular to a detection device and method for Bupleurum chinense antipyretic granules for children. Background Art

[0002] At present, the main ingredients of Xiaoer Chaigui Antipyretic Granules include Bupleurum, cinnamon twig, Pueraria lobata, duckweed, Scutellaria baicalensis, white peony root and cicada molt, which have the effects of diaphoresis, clearing the interior and relieving fever. They are mainly used for children with exogenous fever and have good effects. Because of their significant efficacy and small side effects, they are well received by medical workers and patients. The existing quality standard of Xiaoer Chaigui Antipyretic Granules is to use high performance liquid chromatography to detect the content of puerarin; however, the detection of baicalin content is neglected. Baicalin has the effects of purging fire and removing dampness, and its content is closely related to the effect of Xiaoer Chaigui Antipyretic Granules in treating children with exogenous fever.

[0003] In the related art, the Chinese patent with the publication number CN112326846A proposes a method for detecting the content of baicalin in Xiaoer Chaigui Tuire Granules by HPLC, including preparing the test solution and the reference solution respectively, taking 10 μl of each, injecting into the high performance liquid chromatograph, and calculating the baicalin content by the external standard method after detection. The chromatographic column model is Shimadzu C18, using octadecylsilane bonded silica gel as filler, the mobile phase A is methanol, the mobile phase B is 0.08-0.12% phosphoric acid aqueous solution, and eluting with A: B = 50: 50 (v / v) isocratic. The detection method provided by the above technical scheme has good durability, low baseline noise, high sensitivity; good peak shape, small tailing factor; no other complex pre-treatment is required in the detection process, which can avoid unnecessary interference; low column temperature, mild chromatographic conditions, and convenient operation; it has the advantages of wide applicable concentration range, fast detection speed, high sensitivity, high accuracy and good repeatability, and excellent detection effect.

[0004] With respect to the above-mentioned related technologies, the inventors believe that the following defects exist: when preparing the test samples and reference samples, especially in the process of filtering the test samples to obtain the filtrate, the filtered solids were not analyzed to determine whether there was baicalin that was not dissolved in the methanol in the solids. If baicalin exists in the solid particles, it will affect the detection of the baicalin content. Summary of the invention

[0005] In order to improve the problem that baicalin exists in the solid particles obtained after filtration and affects the detection results, the present application provides a detection device and a detection method for Chaigui antipyretic granules for children.

[0006] The present application provides a device and method for detecting Chaigui antipyretic granules for children, which adopts the following technical solutions: A detection device and method for Xiaoer Chaigui Antipyretic Granules, comprising a sample preparation device for preparing a sample solution and a control sample preparation device for preparing a control solution; The sample preparation device comprises a sample box, a support that is lifted and lowered in the sample box, a filter that moves on the support, a sampling mechanism for sampling solids, and a dissolving mechanism for quickly dissolving baicalin; The sampling inspection mechanism includes a sampling inspection box arranged on the bracket, a reciprocating component for realizing the reciprocating flipping of the filter, a drying component for quickly drying solid particles, and a weighing component for weighing solid particles; the sampling inspection box is provided with a sampling inspection port, and the solid particles enter the sampling inspection port during the flipping process of the filter, and the sampling inspection box is provided with a control component at the sampling inspection port.

[0007] Optionally, the drying component includes a heater, absorbent paper, a winding portion for pulling the absorbent paper, a power portion for realizing reciprocating rotation of the sampling box, and a detection portion for detecting whether the water is dried. The heater is arranged in the bottom wall of the sampling box close to the absorbent paper, the absorbent paper moves along the length direction of the sampling box, and the detection portion and the weighing assembly are respectively located at both ends of the sampling box.

[0008] Optionally, the power unit includes a power motor and two proximity switches, the axis of the rotational connection between the sampling box and the bracket is located on the vertical symmetry plane of the sampling box, the power motor is used to control the rotation of the sampling box, and the two proximity switches are used to control the rotation angle of the sampling box to be no more than 15°.

[0009] Optionally, the detection unit includes a methanol sensor, a fan, a solenoid valve and a gas check valve. The sampling inspection box is provided with an air hole, and an air box is provided at the position of the air hole. The air box is also provided with an air inlet hole, the solenoid valve is arranged in the air inlet hole, the fan is installed in the air box, the methanol sensor is arranged in the air box, the gas check valve is arranged at the air hole, and the heating element controls the operation of the solenoid valve.

[0010] Optionally, the weighing assembly includes a weighing cylinder, a gravity sensor and a weighing plate, the weighing cylinder and the dissolution mechanism are respectively located at two ends of the sampling box, the gravity sensor is installed at the bottom end of the weighing cylinder and is used to connect to the weighing plate, and the gravity sensor is electrically connected to a display screen.

[0011] Optionally, the dissolution mechanism includes a dissolution cage, a driving component for elastically setting the dissolution cage in the sampling box, and a shaking component for accelerating the dissolution of solid particles in the dissolution cage, the dissolution cage and the weighing cylinder are respectively located at two ends of the sampling box, the driving component includes a spring, a driving block, a first electromagnet and a driving member, the spring is used to connect the dissolution cage and the sampling box, the driving block is fixed to the side wall of the dissolution cage, the electromagnet is used to adsorb the driving block, and the driving member is used to control the reciprocating motion of the electromagnet.

[0012] Optionally, a temperature control tube is provided in the side wall of the dissolution cage, and a phase change chamber is provided in the sampling box near the heating element, the bottom end of the phase change chamber is open, a phase change tube is movably provided in the phase change chamber, a traction part for pulling the phase change tube is installed in the phase change chamber, the phase change tube is filled with phase change material, and the bottom end of the phase change chamber is aligned with the top end of the temperature control tube.

[0013] Optionally, the shaking assembly includes a pull rod, a pull rope and non-woven fabric, the area of ​​the non-woven fabric is larger than the cross-sectional area of ​​the dissolving cage, the pull rod is fixed at the center of the dissolving cage, one end of the pull rope is fixedly connected to the bottom end of the pull rod, and the non-woven fabric is arranged at the bottom end of the dissolving cage.

[0014] Optionally, the control sample preparation device includes a control box, a light source arranged at the bottom of the control box, a camera arranged on either side of the control box, a shading mechanism for shading other side walls of the control box, and a processing mechanism for dissolving suspended solid particles, the shading mechanism includes a light sensor, a plurality of shading plates, a plurality of shading ropes, a shading roller and a shading motor, a shading cavity is provided on the corresponding side wall of the control box, the light sensor is installed at the bottom of the shading cavity, the light sensor is electrically connected to the shading motor, and adjacent shading plates are connected by the shading ropes.

[0015] The present application provides a method for detecting Chaigui Antipyretic Granules for Children using the following technical solution: A method for detecting Xiaoer Chaigui Antipyretic Granules comprises the following steps: S1. Preparation of the test solution: weigh the Xiaoer Chaigui Tuire Granules and dissolve them in a methanol-water solution, filter them after ultrasonic treatment, and take the filtrate to obtain the test solution; and perform random inspection on the filtered solid particles to ensure that the solid particles do not contain baicalin; S2. Preparation of reference solution: accurately weigh baicalin reference substance, add it to methanol-water solution, stir well and make up the volume, and ensure that baicalin is completely dissolved in methanol by light detection, so as to obtain a reference solution of known concentration; S3. HPLC determination: Take 10 μl of the test solution and the reference solution respectively, inject them into the high performance liquid chromatograph, and calculate the baicalin content by the external standard method after detection; In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane bonded silica gel is used as filler; the mobile phase A is methanol, and the mobile phase B is 0.08-0.12% phosphoric acid aqueous solution, and the elution is isocratic with A:B=50:50 (v / v).

[0016] In summary, the present application includes at least one of the following beneficial technical effects: 1. When the bracket moves to above the liquid surface, the solid particles on the filter need to be moved into the sampling inspection box, and the end of the filter is in contact with the side wall of the sampling inspection box in the initial state. As the reciprocating member controls the filter to start rotating, the filter tilts toward the sampling inspection box. The filter rotation angle is set to be less than 10°, that is, the gap between the filter and the sampling inspection box can be ignored. Most of the solid particles can fall from the sampling inspection port into the sampling inspection box. At this time, the torsion spring is in a deformed state, and the reciprocating electromagnet is powered off. Under the action of the torsion spring elastic force, the filter returns to its initial state. Under the action of this action, the filter can cause the solid particles above it to flip, greatly accelerating the movement of the solid particles. The reciprocating electric push rod also returns to its initial position under the action of gravity, and controls the reciprocating electromagnet to continue to adsorb the filter. After repeating the last operation many times, the solid particles on the filter are moved into the sampling inspection box. 2. When solid particles enter the sampling box, the sampling box is controlled to swing back and forth and the solid particles are controlled to reciprocate on the absorbent paper. The absorbent paper absorbs methanol. After a period of adsorption, the heating element can be turned on, the absorbent paper is pulled into the winding chamber, and the winding rope is pulled to the bottom wall of the sampling box through the cooperation of two winding rollers. The heating element starts to heat the methanol on the solid particles and evaporate it. Since there may be risks in heating methanol, it is necessary to turn on the fan and the solenoid valve, and control the heating temperature of the heating element. After methanol evaporates, it is drawn into the air box by the fan. If methanol exists, the methanol sensor is controlled by the PLC controller and connected to a buzzer. When the methanol sensor detects methanol vapor, the buzzer is triggered to start working, and the heating element is stopped. It is also necessary to continue to absorb methanol from the solid particles until methanol is no longer present. 3. The camera starts to shoot the methanol solution in the control box. If there are small suspended particles in the control box, it proves that baicalin has been dissolved. If there are more suspended particles in the control box, it is necessary to continue to dissolve baicalin in the methanol solution. First, the filter element is controlled to move toward the position of the methanol liquid surface until the protective rod is plugged into the plug hole and the second electromagnet adsorbs the protective rod. At this time, the processing motor controls the rotation of the rotating rod, and the protective plate on the protective rod moves to the filter plate position. The filter element continues to be lifted, and methanol follows the suspended matter to a position away from the control box. At this time, the rotating rod is quickly controlled to rotate, and methanol will flow out from the filter plate position. However, due to the fast movement speed of the protective plate, the time for stirring and dissolving methanol and baicalin is sufficient. If suspended matter is still found, the above operation is repeated many times to ensure that baicalin can be fully dissolved in methanol and complete the preparation of the solution in the control box. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of a sample preparation device in Example 1 of the present application; Figure 2 It is a partial structural diagram of Example 1 of the present application; Figure 3 It is a schematic diagram of the sampling mechanism and the dissolution mechanism in Example 1 of the present application; Figure 4 yes Figure 3 The enlarged view of point A in the middle; Figure 5 It is a schematic diagram of a control sample preparation device in Example 1 of the present application; Figure 6 is a schematic diagram of the filter element in Example 1 of the present application; Figure 7 It is a schematic diagram of the protection rod and the protection plate in Example 1 of the present application.

[0018] Figure numerals: 1, sample box; 2, bracket; 3, filter screen; 4, random inspection box; 5, random inspection port; 6, control board; 7, reciprocating member; 8, reciprocating block; 9, reciprocating electromagnet; 10, heating member; 11, absorbent paper; 12, winding roller; 13, winding rope; 14, power motor; 15, methanol sensor; 16, fan; 17, solenoid valve; 18, gas one-way valve; 19, gas box; 20, air inlet; 21, air hole; 22, weighing cylinder; 23, gravity sensor; 24, weighing plate; 25, dissolving cage; 26, spring; 27, drive block; 28, first electromagnet; 29, driving member; 30, temperature control tube; 31, phase change chamber; 32, phase change tube; 33, traction rope; 34, traction roller; 35, pull rod; 36, pull rope; 37, non-woven fabric; 38, control box; 39, light source; 40, camera; 41, light sensor; 42, shading plate; 43, shading rope; 44, shading roller; 45, shading chamber; 46, filter element; 47, protective rod; 48, protective plate; 49, rotating rod; 50, second electromagnet; 51, processing motor; 52, filter plate; 53, solid plate. DETAILED DESCRIPTION

[0019] The following is combined with Figure 1-7 This application is described in further detail. Embodiment 1

[0020] The present application embodiment discloses a device for detecting Chaigui antipyretic granules for children. Figure 1-Figure 4A testing device for Xiaoer Chaigui Antipyretic Granules includes a sample preparation device for preparing a sample solution and a control sample preparation device for preparing a control solution; in the process of measuring by HPLC method, it is necessary to prepare a sample and a control, and the sample preparation device is mainly used to ensure that baicalin can be completely dissolved in methanol and will not remain in the solid particles, while the control sample preparation device is mainly used to ensure that the baicalin material can be quickly dissolved in the methanol solution; the sample preparation device includes a sample box 1, a support 2 that is lifted and arranged in the sample box 1, a filter 3 that is movable on the support 2, a sampling mechanism for sampling solids, and a dissolving mechanism for quickly dissolving baicalin; the lifting and lowering of the support 2 can be achieved by an electric push rod or a cylinder, the filter 3 is rotatably arranged on the support 2 and the filter 3 is movable with the support 2 The rotating shaft of the rotatable connection of the frame 2 is located on the symmetrical plane of the filter 3. The sampling mechanism is used to detect the solid particles filtered out by the filter 3. In this embodiment, multiple groups of tests are carried out simultaneously to ensure the accurate detection of the baicalin content. However, during the multiple groups of tests, it is not necessary to conduct a sampling inspection of the solid particles for each group of tests to determine whether baicalin is still present. Therefore, the sampling mechanism conducts a work on whether baicalin is still present in the solid particles produced in one of the groups; the dissolving mechanism dissolves the solid particles filtered out for the first time again. If the weight of the solid particles does not change after dissolution, it proves that there is no excess baicalin in the solid particles. If the weight of the solid particles changes after dissolution, it proves that there is excess baicalin in the solid particles, and the solid particles need to be dissolved multiple times and the baicalin content detection work needs to be re-performed.

[0021] The sampling mechanism includes a sampling box 4 arranged on the bracket 2, a reciprocating component for realizing the reciprocating flipping of the filter 3, a drying component for quickly drying solid particles and a weighing component for weighing solid particles; the sampling box 4 is fixed at either end perpendicular to the rotation axis of the filter 3, and the sampling box 4 is provided with a sampling port 5, and during the rotation of the filter 3, the solid particles enter the sampling box 4 from the sampling port 5, that is, the sampling port 5 is lower than the plane where the filter 3 is located, and the sampling box 4 is located at the lower end of the filter 3 during the rotation process. The sampling box 4 is provided with a control component at the sampling port 5 position, and the control component includes a control panel 6 and an electric push rod. The control panel 6 moves in the vertical direction, and the electric push rod is installed in the sampling box 4. In this embodiment, a protective box needs to be installed in the sampling box 4 to protect the electric push rod, and the control panel 6 is used to control the opening and closing of the sampling port 5.

[0022] The reciprocating assembly includes a reciprocating member 7, a reciprocating block 8, a reciprocating electromagnet 9 and a torsion spring. The shell of the reciprocating member 7 is connected to the bracket 2. The output end of the reciprocating member 7 is also hinged to the reciprocating block 8. The reciprocating electromagnet 9 is fixed on the reciprocating block 8 and is used to adsorb one end of the filter screen 3 away from the sampling box 4. The two ends of the torsion spring respectively fix the filter screen 3 and the bracket 2. When the filter screen 3 is in a horizontal state, the torsion spring is in its original state. When the bracket 2 moves above the liquid surface, the solid particles on the filter screen 3 need to be moved into the sampling box 4, and in the initial state, the end of the filter screen 3 is in contact with the side wall of the sampling box 4. Both ends of the filter screen 3 are set to be arc-shaped to facilitate the swing of the filter screen 3. As the reciprocating member 7 controls the filter screen 3 to start rotating, the filter screen 3 tilts toward the sampling box 4. In this embodiment Since there is a gap between the corresponding end of the filter 3 and the side wall of the sampling box 4 during the rotation of the filter 3, the rotation angle of the filter 3 is set to be less than 10°, that is, the gap between the filter 3 and the sampling box 4 can be ignored, and most of the solid particles can fall from the sampling port 5 into the sampling box 4. At this time, the torsion spring is in a deformed state, and the reciprocating electromagnet 9 is powered off. Under the action of the torsion spring elastic force, the filter 3 returns to its initial state, and under the action of this action, the filter 3 can make the solid particles above it flip, greatly accelerating the movement of the solid particles, and the reciprocating electric push rod also returns to the initial position under the action of gravity, and controls the reciprocating electromagnet 9 to continue to adsorb the filter 3. After repeating the last operation many times, the solid particles on the filter 3 are moved to the sampling box 4.

[0023] The drying assembly includes a heating element 10, absorbent paper 11, a winding portion for pulling the absorbent paper 11, a power portion for realizing the reciprocating rotation of the sampling inspection box 4, and a detection portion for detecting whether the water is dried. The power portion includes a power motor 14 and two proximity switches. The axis of the rotational connection between the sampling inspection box 4 and the bracket 2 is located on the vertical symmetry plane of the sampling inspection box 4. The power motor 14 is used to control the rotation of the sampling inspection box 4. The two proximity switches are used to control the rotation inside the sampling inspection box 4. The proximity switches are connected to the power motor 14 through a PLC controller. The two proximity switches correspond to the sampling inspection boxes 4 and 4, respectively. The two ends of the inspection box 4, that is, when the power motor 14 controls the inspection box 4 to rotate, when the inspection box 4 is located at the higher end and moves close to the proximity switch position, the rotation angle of the inspection box 4 is less than 10°. The purpose of setting 10° is mainly to prevent the solid particles from moving to the two ends. At this time, the solid particles only roll in the middle position of the inspection box 4 and will not roll to the two ends of the inspection box 4. Similarly, when the other end of the inspection box 4 rotates to the corresponding proximity switch, the proximity switch is also triggered, and the proximity switch controls the power motor 14 to rotate in another direction.

[0024] The heating element 10 is arranged on the bottom wall of the sampling inspection box 4 close to the absorbent paper 11. The width of the absorbent paper 11 is adapted to the width of the sampling inspection box 4. The absorbent paper 11 moves along the length direction of the sampling inspection box 4. The detection part and the weighing component are respectively located at the two ends of the sampling inspection box 4. Therefore, the present application ensures that the solid particles roll on the absorbent paper 11 through two proximity switches. The absorbent paper 11 is used to absorb methanol from the solid particles. Methanol can be absorbed by the absorbent paper 11 because the liquid properties of methanol and the water absorption of the absorbent paper 11 are combined, so that the methanol molecules can be absorbed by the fiber structure of the absorbent paper 11 and penetrate into the interior thereof; and the heating element 10 can be an electric heating wire, and the sampling inspection box 4 is provided with a A heating chamber, in which a heating element 10 is installed, a winding portion comprises two winding rollers 12, a plurality of winding ropes 13 and two winding motors. A winding chamber is provided at a position close to the heating chamber of the sampling box 4, in which the winding roller 12 rotates in the winding chamber. A plurality of winding ropes 13 and a plurality of absorbent papers 11 are arranged at intervals. The winding ropes 13 at both ends are respectively fixedly connected to the circumferential walls of the two winding rollers 12. The winding motor is used to control the rotation of the winding roller 12. The heating chamber is provided with two through-grooves for the winding papers to pass through. The through-holes match the absorbent papers 11. In this embodiment, an area of ​​the absorbent paper 11 is set larger than a size between the two through-grooves. Therefore, a portion of the absorbent paper 11 is located in the through-grooves, thereby greatly reducing the probability of methanol entering the through-grooves.

[0025] Reference Figure 1-Figure 4The detection unit includes a methanol sensor 15, a fan 16, a solenoid valve 17 and a gas one-way valve 18. The sampling inspection box 4 is provided with an air hole 21. The sampling inspection box 4 is equipped with an air box 19 at the position of the air hole 21. The fan 16 and the methanol sensor 15 are installed in the air box 19. The air box 19 is also provided with an air inlet 20. The solenoid valve 17 is installed in the air inlet 20. The fan 16 is installed in the air box 19. The methanol sensor 15 is arranged in the air box 19. The gas one-way valve 18 is arranged at the air hole 21. The heating element 10 controls the solenoid valve 17 to work. The heating element 10 and the solenoid valve 17 work through the PLC controller. When solid particles enter the sampling inspection box 4, the sampling inspection box 4 is controlled to swing back and forth and the solid particles are controlled to reciprocate on the absorbent paper 11. The absorbent paper 11 adsorbs methanol. After the adsorption work has been carried out for a period of time, the heating element 10 can be turned on, the absorbent paper 11 is pulled into the winding chamber, and the two winding rollers 12 cooperate with each other to pull the winding rope 13 to the bottom wall of the sampling box 4, and the heating element 10 begins to heat the methanol on the solid particles and evaporate it. Since there may be risks in heating methanol, it is necessary to turn on the fan 16 and the solenoid valve 17, and control the heating temperature of the heating element 10. After methanol evaporates, it is drawn into the air box 19 by the fan 16. If methanol exists, the methanol sensor 15 is controlled by the PLC controller and is connected to a buzzer. When the methanol sensor 15 detects methanol vapor, the buzzer is triggered to start working, and the heating element 10 stops working. It is also necessary to continue to absorb methanol from the solid particles until methanol is no longer present.

[0026] Reference Figure 1-Figure 4 The weighing assembly includes a weighing cylinder 22, a gravity sensor 23 and a weighing plate 24. After the methanol absorption work is completed, the solid particles need to be weighed. The weighing cylinder 22 and the dissolving mechanism are respectively located at the two ends of the sampling box 4. The gravity sensor 23 is installed at the bottom end of the weighing cylinder 22 and is used to connect the weighing plate 24. The gravity sensor 23 is electrically connected to a display screen, and the gravity sensor 23 and the display screen are controlled and connected through a PLC controller; the power motor 14 controls the sampling box 4 to rotate from a horizontal state to a vertical state and controls the weighing cylinder 22 to be located at the bottom. The weighing plate 24 is used to receive solid particles. At this time, the gravity sensor 23 displays the weight of the solid particles at this time through the display screen, and the staff reads and records it.

[0027] The dissolving mechanism includes a dissolving cage 25, a driving component for realizing elastic setting of the dissolving cage 25 in the sampling box 4, and a shaking component for accelerating the dissolution of solid particles in the dissolving cage 25. The dissolving cage 25 and the weighing cylinder 22 are respectively located at the two ends of the sampling box 4. The driving component includes a spring 26, a driving block 27, a first electromagnet 28 and a driving member 29. The dissolving cage 25 is fixedly connected with a slide rod. The inner wall of the sampling box 4 is provided with a slide hole for sliding the slide rod. The two ends of the spring 26 are respectively fixedly connected with one end of the slide rod and the inner wall of the end of the slide hole to realize the effect of elastic connection between the dissolving cage 25 and the sampling box 4. The driving block 27 is fixed on the side wall of the dissolving cage 25. The electromagnet is used to adsorb the driving block 27. The driving member 29 is used to control the reciprocating motion of the electromagnet. The driving member 29 can be an electric push rod or a cylinder, and in this embodiment, the driving member 29 is also A protective box is required to protect it; in the initial state, the electromagnet is adsorbed with the driving block 27, and the spring 26 is in a deformed state. When the solid particles enter the dissolution cage 25, the aperture on the dissolution cage 25 is smaller than the size of the solid particles, and the solid particles will only dissolve in the methanol solution and will not fall out of the dissolution cage 25. Before the first electromagnet 28 is powered off, it is necessary to control the position of the bracket 2 to ensure that the dissolution cage 25 can enter the methanol solution after the final release, and then the first electromagnet 28 can be powered off. Under the elastic force of the spring 26, the dissolution cage 25 is ejected into the methanol solution, and methanol begins to dissolve the solid particles again. Moreover, since the dissolution cage 25 is ejected into the methanol solution, the solid particles are also suspended in the methanol solution from the inner wall of the dissolution cage 25 under the impact of methanol, which greatly accelerates the dissolution work.

[0028] Reference Figure 1-Figure 4A temperature control tube 30 is arranged in the side wall of the dissolving cage 25, and a phase change chamber 31 is arranged in the sampling box 4 near the position of the heating element 10. The bottom end of the phase change chamber 31 is opened, and a phase change tube 32 is movably arranged in the phase change chamber 31. A traction part for pulling the phase change tube 32 is installed in the phase change chamber 31. The phase change tube 32 is filled with phase change material. The bottom end of the phase change chamber 31 is aligned with the top end of the temperature control tube 30. The length direction of the heating chamber is perpendicular to the movement direction of the winding rope 13, and the length direction of the phase change chamber 31 is parallel to the length direction of the heating chamber. The traction part includes a traction motor, a traction roller 34 and a traction rope 33. The traction motor is fixed at the top end of the phase change chamber 31, and the traction roller 34 rotates with the top end of the phase change chamber 31. The phase change tube 32 is connected, one end of the traction rope 33 is fixedly connected to the peripheral wall of the traction roller 34, and the phase change tube 32 is always in a vertical state during the whole process, and before the dissolution cage 25 is separated from the sampling box 4, the phase change tube 32 is controlled to gradually fall, and the phase change tube 32 moves into the temperature control tube 30, and it is necessary to ensure that the winding rope 13 is in a relaxed state to prevent the phase change tube 32 from falling from the temperature control tube 30. The phase change tube 32 begins to absorb the heat of the heating element 10 when it is in the phase change chamber 31. When the dissolution cage 25 enters into the methanol solution, the phase change tube 32 begins to release heat, and quickly heats the methanol solution in and near the dissolution cage 25. If baicalin exists in the solid particles, the dissolution rate of baicalin can be accelerated.

[0029] Reference Figure 1-Figure 4 The shaking assembly includes a pull rod 35, a pull rope 36 and a non-woven fabric 37. The area of ​​the non-woven fabric is larger than the cross-sectional area of ​​the dissolution cage 25. The pull rod 35 is fixed in the sampling box 4 and is located at the center of the dissolution cage 25. One end of the pull rope 36 is fixedly connected to the bottom end of the pull rod 35. The non-woven fabric 37 is arranged at the bottom end of the dissolution cage 25. The non-woven fabric 37 is in a naturally drooping state in the initial state. When the dissolution cage 25 impacts the methanol solution, most of the solid particles will float toward the methanol solution. In order to make all the solid particles float toward the methanol solution, as the dissolution cage 25 continues to move toward the methanol solution, the non-woven fabric 37 is quickly pulled upward by the pull rope 36. The non-woven fabric 37 has a shaking force. At this time, the solid particles are shaken from the non-woven fabric 37 into the methanol solution, further accelerating the speed at which the solid particles continue to dissolve in methanol. After repeating the above operation, the dissolution cage 25 is pulled out of the water, the sampling box 4 is rotated to a horizontal position, and after the methanol is adsorbed by absorbent paper 11, the dissolved solid particles are weighed by the gravity sensor 23. If the weight changes, baicalin exists in the solid particles; if the weight of the two weighings does not change, baicalin does not exist in the normal solid particles.

[0030] Reference Figure 5-Figure 7The control sample preparation device includes a control box 38, a light source 39 arranged at the bottom of the control box 38, a camera 40 arranged on either side of the control box 38, a shading mechanism for shading other side walls of the control box 38, and a processing mechanism for dissolving suspended solid particles. First, the shading mechanism shades the side walls of the control box 38, and the light source 39 is turned on. If there are suspended matter in the methanol solution, it proves that baicalin is not completely dissolved, and the processing mechanism is used to accelerate the dissolution of baicalin; the shading mechanism includes a light sensor 41, a plurality of shading plates 42, a plurality of shading ropes 43, a shading roller 44 and a shading motor. The corresponding side wall of the control box 38 is provided with a shading cavity 45, the light sensor 41 is provided with the bottom of the shading cavity 45 and is used to identify the light of the light source 39, and the material selection of the control box 38 It is made of transparent material, adjacent shading plates 42 are connected by shading ropes 43, and the light sensor 41 is electrically connected to the shading motor. The light sensor 41 and the shading motor are controlled and connected by a PLC controller. After the light sensor 41 recognizes the illumination of the light source 39, the shading motor starts to work quickly, and the shading motor starts to control the shading roller 44 to start rotating. The shading roller 44 starts to continuously lower the wound shading plate 42, and the shading plate 42 starts to block the side wall of the control box 38 to reduce the influence of the ambient light on the shooting of the camera 40. The camera 40 starts to shoot the methanol solution in the control box 38. If the suspended particles in the control box 38 are relatively small, it proves that the baicalin has been dissolved. If there are more suspended particles in the control box 38, it is necessary to continue the operation to dissolve the baicalin in the methanol solution.

[0031] Reference Figure 5-Figure 7The processing mechanism includes a filter element 46 that is lifted and arranged in the control box 38, a protective rod 47 that is rotatably arranged in the filter element 46, a plurality of protective plates 48 fixed on the outer peripheral wall of the protective rod 47, a rotating rod 49, a second electromagnet 50 and a processing motor 51. The lifting and lowering of the filter element 46 is realized by an electric push rod or a cylinder. The protective rod 47 rotates at the center position of the filter element 46. The processing motor 51 is installed on the top of the control box 38 and is used to control the rotation of the rotating rod 49. The rotating rod 49 is provided with a plug-in hole for plugging in the protective rod 47. In this embodiment, the protective rod 47 and the plug-in hole are The cross-sections are all set to be square, and the second electromagnet 50 is installed on the top inner wall of the plug hole. The second electromagnet 50 is used to adsorb the protective rod 47, that is, when the protective rod 47 is plugged into the plug hole, the protective rod 47 can be driven by the rotating rod 49 to rotate together. The protective plate 48 is arranged at equal intervals on the outer peripheral wall of the protective rod 47. The filter 46 includes a plurality of filter plates 52 and a plurality of solid plates 53. In this embodiment, the solid plates 53 and the filter plates 52 are set to three in number, so the number of the protective plates 48 is also set to three. The filter plate 52 is provided with a plurality of filter holes for filtering baicalin. Filtering, while no filter holes are set on the solid plate 53, the protective plate 48 corresponds to the solid plate 53, and the bottom of the protective plate 48 is close to the top of the solid plate 53, and the size of the protective plate 48 is larger than the size of the filter plate 52, so as to ensure that when the protective plate 48 moves to the position of the filter plate 52, the methanol moves with the filter element 46; when the light finds that there are more suspended matter in the control box 38, it is necessary to accelerate the dissolution of the suspended matter. In this embodiment, the filter element 46 is first controlled to move toward the position of the methanol liquid surface until the protective rod 47 is plugged into the plug hole and the second electromagnet 50 is used to drive the protective rod 47. Adsorption is carried out, at this time, the processing motor 51 is used to control the rotation of the rotating rod 49, and the protective plate 48 on the protective rod 47 moves to the filter plate 52 position, and the filter element 46 continues to be lifted. Methanol follows the suspended matter and moves to a position away from the control box 38. At this time, the rotating rod 49 is quickly controlled to rotate, and methanol will flow out from the filter plate 52 position. However, since the protective plate 48 moves quickly, the time for stirring and dissolving methanol and baicalin is sufficient. If suspended matter is still found, the above operation is repeated for multiple times to ensure that baicalin can be fully dissolved in methanol and complete the preparation of the solution in the control box 38.

[0032] The implementation principle of the pediatric Chaigui antipyretic particle detection device of the embodiment of the present application is as follows: when the bracket 2 moves above the liquid surface, the solid particles on the filter screen 3 need to be moved into the sampling inspection box 4, and in the initial state, the end of the filter screen 3 is in contact with the side wall of the sampling inspection box 4, and as the reciprocating member 7 controls the filter screen 3 to start rotating, the filter screen 3 is tilted toward the sampling inspection box 4, and the rotation angle of the filter screen 3 is set to be less than 10°, that is, the gap between the filter screen 3 and the sampling inspection box 4 can be ignored, and most of the solid particles can fall from the sampling inspection port 5 into the sampling inspection box 4, and at this time, the torsion spring is in a deformed state, and the reciprocating electromagnet 9 is powered off. Under the action of the torsion spring elastic force, the filter screen 3 returns to its initial state, and under the action of this action, the filter screen 3 can cause the solid particles above it to flip, greatly accelerating the movement of the solid particles, and the reciprocating electric push rod also returns to the initial position under the action of gravity, and controls the reciprocating electromagnet 9 to continue to adsorb the filter screen 3. After repeating the last operation many times, the solid particles on the filter screen 3 are moved to the sampling inspection box 4.

[0033] When solid particles enter the sampling box 4, the sampling box 4 is controlled to swing back and forth and the solid particles are controlled to reciprocate on the absorbent paper 11. The absorbent paper 11 absorbs methanol. After the adsorption work is carried out for a period of time, the heating element 10 can be turned on, the absorbent paper 11 is pulled into the winding chamber, and the winding rope 13 is pulled to the bottom wall of the sampling box 4 through the cooperation of the two winding rollers 12. The heating element 10 starts to heat the methanol on the solid particles and evaporate it. Since there may be risks in the heating of methanol, it is necessary to turn on the fan 16 and the solenoid valve 17, and control the heating temperature of the heating element 10. After methanol evaporates, it is drawn into the air box 19 by the fan 16. If methanol exists, the methanol sensor 15 is controlled by the PLC controller and is connected to a buzzer. When the methanol sensor 15 detects methanol vapor, the buzzer is triggered to start working, and the heating element 10 stops working. It is also necessary to continue to absorb methanol from the solid particles until methanol does not exist. In the initial state, the first electromagnet 28 is attracted to the driving block 27, and the spring 26 is in a deformed state. When the solid particles enter the dissolution cage 25, the phase change tube 32 begins to release heat, and the methanol solution in and near the dissolution cage 25 is quickly heated. If baicalin exists in the solid particles, the dissolution rate of baicalin can be accelerated. As the dissolution cage 25 continues to move toward the methanol solution, the non-woven fabric 37 is pulled The rope 36 is pulled upward quickly, and the non-woven fabric 37 has a shaking force. At this time, the solid particles are shaken from the non-woven fabric 37 into the methanol solution, further accelerating the speed at which the solid particles continue to dissolve in methanol; after repeating the above operation, the dissolution cage 25 is pulled out of the water, the sampling box 4 is rotated to a horizontal position, and after the methanol is adsorbed by absorbent paper 11, the dissolved solid particles are weighed by the gravity sensor 23. If the weight changes, baicalin exists in the solid particles; if the weight of the two weighings does not change, baicalin does not exist in the normal solid particles.

[0034] The camera 40 starts to shoot the methanol solution in the control box 38. If there are relatively small suspended particles in the control box 38, it proves that baicalin has been dissolved. If there are more suspended particles in the control box 38, it is necessary to continue to dissolve baicalin in the methanol solution. First, the filter element 46 is controlled to move toward the position of the methanol liquid surface until the protective rod 47 is plugged into the plug hole and the second electromagnet 50 adsorbs the protective rod 47. At this time, the processing motor 51 controls the rotating rod 49 to rotate, and the protective plate 48 on the protective rod 47 moves to the filter plate 52 position. The filter element 46 continues to be lifted, and methanol follows the suspended matter to a position away from the control box 38. At this time, the rotating rod 49 is quickly controlled to rotate, and methanol will flow out from the filter plate 52 position. However, since the protective plate 48 moves quickly, the time for stirring and dissolving methanol and baicalin is sufficient. If suspended matter is still found, the above operation is repeated for multiple times to ensure that baicalin can be fully dissolved in methanol and complete the preparation of the solution in the control box 38. Embodiment 2

[0035] The present application example discloses a method for detecting Xiaoer Chaigui Antipyretic Granules. Figure 1 A method for detecting Xiaoer Chaigui Antipyretic Granules comprises the following steps: S1. Preparation of the test solution: weigh the Xiaoer Chaigui Tuire Granules and dissolve them in a methanol-water solution, filter them after ultrasonic treatment, and take the filtrate to obtain the test solution; and perform random inspection on the filtered solid particles to ensure that the solid particles do not contain baicalin; by dissolving the filtered solid particles again, and comparing whether there is a weight change after the two dissolutions, it is determined whether the solid particles contain baicalin. If the random inspection process proves that the solid particles contain baicalin, it is necessary to prepare the test sample again to ensure the accuracy of the baicalin content detection; S2. Preparation of reference solution: accurately weigh the baicalin reference substance, add it to the methanol-water solution, stir it evenly and make up the volume, and ensure that the baicalin is completely dissolved in the methanol by light detection, so as to obtain a reference solution of known concentration; and if the baicalin is not fully dissolved, the suspended particles are taken out and stirred again to dissolve, which greatly improves the solubility and dissolution efficiency of the baicalin; S3. HPLC determination: Take 10 μl of the test solution and the reference solution respectively, inject them into the high performance liquid chromatograph, and calculate the baicalin content by the external standard method after detection; In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane bonded silica gel is used as filler; mobile phase A is methanol, and mobile phase B is 0.08-0.12% phosphoric acid aqueous solution, and isocratic elution is performed with A:B=50:50 (v / v). The use of the above-mentioned chromatographic column model can better achieve the separation of baicalin and other components in Xiaoer Chaigui Tuire Granules, with good peak shape and small tailing factor; this model of chromatographic column uses octadecylsilane bonded silica gel as filler, which can achieve excellence and durability, and obtain good chromatographic peak shape.

[0036] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A testing device for Chaigui antipyretic granules for children, Features: It includes a sample preparation device for preparing a test solution and a control sample preparation device for preparing a reference solution; The sample preparation device comprises a sample box (1), a support (2) which is lifted and arranged in the sample box (1), a filter (3) which is movable on the support (2), a sampling mechanism for sampling solids, and a dissolving mechanism for quickly dissolving baicalin; The sampling inspection mechanism comprises a sampling inspection box (4) arranged on the support (2), a reciprocating component for realizing the reciprocating flipping of the filter screen (3), a drying component for quickly drying solid particles, and a weighing component for weighing solid particles; the sampling inspection box (4) is provided with a sampling inspection port (5), and solid particles enter the sampling inspection port (5) during the flipping process of the filter screen (3); the sampling inspection box (4) is provided with a control component at the position of the sampling inspection port (5).

2. A detection device for Xiaoer Chaigui Antipyretic Granules according to claim 1, Features: The drying component comprises a heating element (10), absorbent paper (11), a winding portion for pulling the absorbent paper (11), a power portion for realizing reciprocating rotation of the sampling inspection box (4), and a detection portion for detecting whether the water is dried. The heating element (10) is arranged in the bottom wall of the sampling inspection box (4) close to the absorbent paper (11), the absorbent paper (11) moves along the length direction of the sampling inspection box (4), and the detection portion and the weighing component are respectively located at two ends of the sampling inspection box (4).

3. A detection device for Xiaoer Chaigui Antipyretic Granules according to claim 2, Features: The power unit comprises a power motor (14) and two proximity switches. The axis of the rotational connection between the sampling box (4) and the bracket (2) is located on the vertical symmetry plane of the sampling box (4). The power motor (14) is used to control the rotation of the sampling box (4). The two proximity switches are used to control the rotation angle of the sampling box (4) to be no greater than 15°.

4. A detection device for Xiaoer Chaigui Antipyretic Granules according to claim 3, Features: The detection unit comprises a methanol sensor (15), a fan (16), a solenoid valve (17) and a gas one-way valve (18); the sampling inspection box (4) is provided with an air hole (21); the sampling inspection box (4) is provided with an air box (19) at the position of the air hole (21); the air box (19) is also provided with an air inlet hole (21); the solenoid valve (17) is arranged in the air inlet hole (20); the fan (16) is installed in the air box (19); the methanol sensor (15) is arranged in the air box (19); the gas one-way valve (18) is arranged at the air hole (21); and the heating element (10) controls the operation of the solenoid valve (17).

5. A detection device for Xiaoer Chaigui Antipyretic Granules according to claim 4, Features: The weighing assembly comprises a weighing cylinder (22), a gravity sensor (23) and a weighing plate (24); the weighing cylinder (22) and the dissolving mechanism are respectively located at two ends of the sampling box (4); the gravity sensor (23) is installed at the bottom end of the weighing cylinder (22) and is used to connect to the weighing plate (24); the gravity sensor (23) is electrically connected to a display screen.

6. A device for detecting the Xiaoer Chaigui antipyretic granules according to claim 5, Features: The dissolution mechanism includes a dissolution cage (25), a driving component for realizing the elastic setting of the dissolution cage (25) in the sampling box (4), and a shaking component for accelerating the dissolution of solid particles in the dissolution cage (25). The dissolution cage (25) and the weighing cylinder (22) are respectively located at two ends of the sampling box (4). The driving component includes a spring (26), a driving block (27), a first electromagnet (28) and a driving member (29). The spring (26) is used to connect the dissolution cage (25) and the sampling box (4). The driving block (27) is fixed to the side wall of the dissolution cage (25). The electromagnet is used to adsorb the driving block (27). The driving member (29) is used to control the reciprocating motion of the electromagnet.

7. A detection device for Xiaoer Chaigui Antipyretic Granules according to claim 6, Features: A temperature control tube (30) is arranged in the side wall of the dissolution cage (25), and a phase change chamber (31) is arranged in the sampling box (4) at a position close to the heating element (10). The bottom end of the phase change chamber (31) is opened, and a phase change tube (32) is movably arranged in the phase change chamber (31). A traction part for pulling the phase change tube (32) is installed in the phase change chamber (31), and a phase change material is installed in the phase change tube (32). The bottom end of the phase change chamber (31) is aligned with the top end of the temperature control tube (30).

8. A device for detecting the Xiaoer Chaigui antipyretic granules according to claim 7, Features: The shaking assembly includes a pull rod (35), a pull rope (36) and a non-woven fabric (37), the area of ​​the non-woven fabric is larger than the cross-sectional area of ​​the dissolving cage (25), the pull rod (35) is fixed at the center of the dissolving cage (25), one end of the pull rope (36) is fixedly connected to the bottom end of the pull rod (35), and the non-woven fabric (37) is arranged at the bottom end of the dissolving cage (25).

9. A device for detecting the Xiaoer Chaigui antipyretic granules according to claim 8, Features: The control sample preparation device comprises a control box (38), a light source (39) arranged at the bottom of the control box (38), a camera (40) arranged on either side of the control box (38), a shading mechanism for shading other side walls of the control box (38), and a processing mechanism for dissolving suspended solid particles. The shading mechanism comprises a light sensor (41), a plurality of shading plates (42), a plurality of shading ropes (43), a shading roller (44) and a shading motor. A shading cavity (45) is arranged on the corresponding side wall of the control box (38). The light sensor (41) is installed at the bottom of the shading cavity (45). The light sensor (41) is electrically connected to the shading motor. Adjacent shading plates (42) are connected via the shading ropes (43).

10. A method for detecting Chaigui Antipyretic Granules for Children, based on the Chaigui Antipyretic Granules for Children detection device according to any one of claims 1 to 9, Features: The steps include: S1. Preparation of the test solution: weigh the Xiaoer Chaigui Tuire Granules and dissolve them in a methanol-water solution, filter them after ultrasonic treatment, and take the filtrate to obtain the test solution; and perform random inspection on the filtered solid particles to ensure that the solid particles do not contain baicalin; S2. Preparation of reference solution: accurately weigh baicalin reference substance, add it to methanol-water solution, stir well and make up the volume, and ensure that baicalin is completely dissolved in methanol by light detection, so as to obtain a reference solution of known concentration; S3. HPLC determination: Take 10 μl of the test solution and the reference solution respectively, inject them into the high performance liquid chromatograph, and calculate the baicalin content by the external standard method after detection; In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane bonded silica gel is used as filler; the mobile phase A is methanol, and the mobile phase B is 0.08-0.12% phosphoric acid aqueous solution, and the elution is isocratic with A:B=50:50 (v / v).

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