Low-temperature freezing equipment for ovarian tissue preservation

Abstract

The invention relates to the technical field of ovarian tissue preservation, in particular to low-temperature freezing equipment for ovarian tissue preservation, the lower portion of a tissue slice is blocked and limited through a limiting block, the problem that the tissue slice is stacked together due to vibration or liquid impact force is avoided, and therefore interference on freezing preservation operation of the tissue slice is avoided; the invention discloses low-temperature freezing equipment for ovarian tissue preservation. The low-temperature freezing equipment comprises a sieve, a long needle and a cylinder, the sieve is fixedly connected with at least two long needles; a cylinder is arranged on the side of the sieve; the device further comprises a limiting block, a housing I and a decompression filler; the cylinder is fixedly connected with at least five groups of limiting blocks, and each group comprises at least four limiting blocks; the lengths of the limiting blocks are gradually increased from top to bottom; the cylinder is connected with a housing I; a plurality of liquid permeable holes are formed in the housing I; and the inner side of the housing I is provided with decompression filler.

Description

Technical Field

[0001] The present invention relates to the technical field of ovarian tissue preservation, and more specifically, to a low-temperature freezing device for ovarian tissue preservation. Background Art

[0002] The main functions of the ovaries include producing eggs and secreting female hormones, such as estrogen and progesterone, which play a vital role in maintaining female secondary sexual characteristics, regulating the menstrual cycle and the process of pregnancy;

[0003] Ovarian tissue preservation is relatively difficult, therefore, a Chinese patent with publication number "CN101314757B" discloses a needle-pierced tissue slice vitrification freezing carrier and a method for freezing ovarian tissue, wherein multiple ovarian tissue slices can be strung in a row on a steel needle at intervals, and then the ovarian tissue slices are immersed in liquid nitrogen for preservation;

[0004] However, the ovarian tissue slices are not stable after being inserted into the steel needles at intervals. When the ovarian tissue slices are vibrated or impacted by liquid, they will slide down, causing the ovarian tissue slices to stack together, which in turn makes it impossible for the liquid nitrogen to fully immerse each ovarian tissue slice, thus interfering with the freezing and preservation operation of the ovarian tissue slices. Summary of the invention

[0005] In order to overcome the disadvantage that when ovarian tissue slices are subjected to vibration or liquid impact, the ovarian tissue slices may be stacked together, thereby interfering with the cryopreservation operation of the ovarian tissue slices, the present invention provides a low-temperature freezing device for ovarian tissue preservation.

[0006] The technical solution is: a low-temperature freezing device for preserving ovarian tissue, including a sieve, a long needle and a cylinder; at least two long needles are fixedly connected to the sieve; a cylinder is arranged on the side of the sieve; it also includes a limit block, a cover shell and a decompression filler; at least five groups of limit blocks are fixedly connected to the cylinder, and each group of limit blocks is arranged with at least four; the length of the limit blocks is arranged in an increasing manner from top to bottom; the cylinder is connected to the cover shell; a plurality of liquid permeable holes are opened on the cover shell; and a decompression filler is arranged on the inner side of the cover shell.

[0007] As a further preferred embodiment, the sieve consists of an upper cylinder and a lower cone.

[0008] As a further preferred embodiment, the opening on the cylinder is trumpet-shaped.

[0009] As a further preferred embodiment, a transfer unit is also included; the transfer unit is connected to the sieve; the transfer unit includes a threaded column and a handle; the sieve is fixed with a threaded column; the threaded column is screwed with a handle, and the handle is in contact with the sieve.

[0010] As a further preferred embodiment, it also includes an auxiliary unit; the auxiliary unit is connected to the cylinder; the auxiliary unit includes a cover shell 2, a long rod and a block; the cylinder is connected to the cover shell 2 in a damping sliding manner, and the cover shell 1 is fixedly connected to the inner side of the cover shell 2; the cover shell 1 is connected to the long rod in a damping sliding manner; the long rod is fixedly connected to the block; the block is in contact with the cover shell 2; a cavity 1 is formed between the upper side of the cover shell 1 and the cover shell 2, and the cavity 1 is connected to the corresponding liquid-permeable hole; a cavity 2 is formed between the lower side of the cover shell and the cover shell 2, and the cavity 2 is connected to the corresponding liquid-permeable hole; a circular hole 1 is opened on the upper side of the cover shell 2, and the circular hole 1 is connected to the cavity 1; a circular hole 2 is opened on the lower side of the cover shell 2, and the circular hole 2 is connected to the cavity 2.

[0011] As a further preferred solution, it also includes a threaded cover; the threaded cover is fixedly connected to the handle; a thread is arranged on the lower part of the outer side of the cylinder; and the threaded cover matches the cylinder.

[0012] As a further preferred scheme, it also includes a reinforcement unit; the reinforcement unit is connected to the long rod; the reinforcement unit includes a spring, a linkage block and a threaded block; the spring is fixed to the long rod; the linkage block is fixed to the spring, and the linkage block is slidably connected to the long rod; at least two threaded blocks are fixed to the linkage block; another type of thread is provided in the middle of the threaded cover; the pitch of the thread on the outer side of the threaded cover is greater than the pitch of the thread in the middle of the threaded cover; the threaded block cooperates with the threaded cover.

[0013] As a further preferred solution, the edge of the upper side of the threaded column is chamfered.

[0014] As a further preferred solution, the inner edge of the lower side of the handle is chamfered.

[0015] As a further preferred solution, an anti-slip sleeve is provided on the outer side of the threaded cover.

[0016] The beneficial effects are:

[0017] 1. The limit block is used to block the bottom of the tissue slice to prevent the tissue slices from being stacked together due to vibration or liquid impact, thereby avoiding interference with the cryopreservation operation of the tissue slices;

[0018] Second, compared with directly immersing the tissue slices from top to bottom in liquid nitrogen, the friction between the cylinder and the cover shell 2 can make the liquid nitrogen slowly move upward to immerse the tissue slices, which greatly reduces the impact force on the tissue slices, is conducive to improving the stability of the tissue slices on the long needle, and further avoids interference with the freezing and preservation operation of the tissue slices. At the same time, the handle can be fixed to the lower part of the inner side of the cylinder through the threaded cover, and then the preservation operation is carried out, which is conducive to improving the space utilization rate of the liquid nitrogen tank;

[0019] 3. When manually tightening the threaded cover on the cylinder, the threaded cover will also drive the threaded block to move downward, thereby pressing the stopper against the cover 1 to prevent the stopper from sliding freely and blocking the circular hole 1, thereby avoiding interference with the liquid nitrogen exchange operation inside and outside the cylinder. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The schematic diagram of the structure of the low-temperature freezing device for ovarian tissue preservation of the present invention is shown;

[0021] Figure 2 A diagram showing the combined state of the low-temperature freezing device for ovarian tissue preservation of the present invention;

[0022] Figure 3 The schematic diagram of the structure of the transfer unit of the present invention is shown;

[0023] Figure 4 A schematic structural diagram of a limit block of the present invention is shown;

[0024] Figure 5 A schematic diagram of the structure of the auxiliary unit of the present invention is shown;

[0025] Figure 6 A schematic structural diagram of a reinforcement unit of the present invention is shown;

[0026] Figure 7 A front view showing the assembled state of the low-temperature freezing device for ovarian tissue preservation of the present invention;

[0027] Figure 8 The present invention is shown Figure 7 The enlarged view of point A in the middle;

[0028] Fig. 9 The present invention is shown Figure 7 Enlarged view of point B in the middle.

[0029] In the accompanying drawings: 1-sieve, 2-long needle, 3-cylinder, 4-limiting block, 5-cover shell one, 6-decompression filler, 7-threaded column, 8-handle, 9-threaded cover, 10-cover shell two, 11-long rod, 12-stopper, 13-spring, 14-linkage block, 15-threaded block, 16-tissue section, 91-liquid permeable hole, 92-cavity one, 93-cavity two, 94-round hole one, 95-round hole two. DETAILED DESCRIPTION

[0030] The present invention will be further described below in conjunction with the embodiments shown in the accompanying drawings.

[0031] Example 1

[0032] A cryogenic freezing device for preserving ovarian tissue, such as Figure 1-Figure 5 and Figure 7As shown, it includes a sieve 1, a long needle 2 and a cylinder 3; two long needles 2 are fixedly connected to the sieve 1, and the long needles 2 are set to be made of alloy material; a cylinder 3 is arranged on the side of the sieve 1; it also includes a limit block 4, a cover 5 and a pressure reducing filler 6; five groups of limit blocks 4 are fixedly connected to the cylinder 3, and each group of limit blocks 4 is provided with four; the lengths of the limit blocks 4 are arranged in an increasing manner from top to bottom; the cylinder 3 is connected to the cover 5; a plurality of liquid permeable holes 91 are opened on the cover 5; a pressure reducing filler 6 is arranged on the inner side of the cover 5.

[0033] The sieve 1 is composed of an upper cylindrical body and a lower conical body, so that the sieve 1 can be inserted into the cylinder 3 more easily.

[0034] The opening on the cylinder 3 is in a trumpet shape, so that the sieve 1 is easier to insert into the cylinder 3 .

[0035] It also includes a transfer unit; the transfer unit is connected to the sieve 1; the transfer unit includes a threaded column 7 and a handle 8; the threaded column 7 is fixed to the sieve 1, and the threaded column 7 is set to an alloy material; the handle 8 is screwed on the threaded column 7, and the handle 8 is in contact with the sieve 1. The handle 8 is manually grasped to transfer the sieve 1 and the parts thereon.

[0036] First, the pre-treated ovarian tissue is cut into five tissue slices 16 with decreasing diameters by manually using circular mold knives of different specifications, and then the five tissue slices 16 are threaded onto the long needle 2 in order from large to small. At this time, the five tissue slices 16 are in the following state: Figure 3 As shown, liquid nitrogen is then poured into the cylinder 3, the liquid nitrogen level is one centimeter higher than the uppermost limit block 4, and then the handle 8 is manually held to drive the handle 8 and the parts thereon to move to just above the cylinder 3, and then the handle 8 and the parts thereon are driven to move downward, so that the long needle 2 drives the tissue slice 16 thereon to immerse into the liquid nitrogen, and at the same time, the sieve 1 is inserted into the upper opening of the cylinder 3, the upper opening is blocked, and then the device is placed in a liquid nitrogen tank for storage. During the storage process, due to the temperature difference between the inside and outside of the cylinder 3, a pressure difference occurs between the inside and outside of the cylinder 3. At this time, the liquid nitrogen flows through the liquid permeable hole 91 on the cover 5 and the decompression filler 6, so that the pressure inside and outside of the cylinder 3 tends to be balanced; during this process, the tissue slice 16 and the limit block 4 are Counting, counting from top to bottom is the first to the fifth. Since the length of the limit block 4 is set to increase from top to bottom, and the diameter of the tissue slice 16 is set to decrease from top to bottom, the fifth tissue slice 16 can pass through the first to fourth groups of limit blocks 4 downward and be blocked by the fifth group of limit blocks 4, the fourth tissue slice 16 can pass through the first to third groups of limit blocks 4 downward and be blocked by the fourth group of limit blocks 4, the third tissue slice 16 can pass through the first and second groups of limit blocks 4 downward and be blocked by the third group of limit blocks 4, the second tissue slice 16 can pass through the first group of limit blocks 4 downward and be blocked by the second group of limit blocks 4, and the first tissue slice 16 is blocked by the first group of limit blocks 4. At this time, the states of the limit blocks 4 and the tissue slices 16 are as follows: Figure 7 As shown, the limit block 4 blocks and limits the bottom of the tissue slices 16 to avoid stacking that affects the storage operation. When in use, the limit block 4 blocks and limits the bottom of the tissue slices 16 to avoid the problem of the tissue slices 16 being stacked together due to vibration or liquid impact, thereby avoiding interference with the freezing storage operation of the tissue slices 16.

[0037] Example 2

[0038] On the basis of Example 1, Figure 5 , Figure 7 and Figure 8 As shown, it also includes an auxiliary unit; the cylinder 3 is connected to the auxiliary unit; the auxiliary unit includes a cover 2 10, a long rod 11 and a stopper 12; the cylinder 3 is connected to the cover 2 10 in a damping sliding manner, and the cover 1 5 is fixed to the inner side of the cover 2 10; the cover 1 5 is connected to the long rod 11 in a damping sliding manner; the stopper 12 is welded to the long rod 11; the stopper 12 is in contact with the cover 2 10; the upper side of the cover 1 5 and the cover 2 10 form a A cavity 92 is formed, and the cavity 92 is connected to the corresponding liquid-permeable hole 91; a cavity 93 is formed between the lower side of the cover shell 15 and the cover shell 2 10, and the cavity 93 is connected to the corresponding liquid-permeable hole 91; a circular hole 94 is formed on the upper side of the cover shell 2 10, and the circular hole 94 is connected to the cavity 92; a circular hole 95 is formed on the lower side of the cover shell 2 10, and the circular hole 95 is connected to the cavity 93, and the circular hole 94 is covered by the block 12.

[0039] It also includes a threaded cover 9; the threaded cover 9 is bolted to the handle 8; the lower outer portion of the cylinder 3 is provided with threads; the threaded cover 9 cooperates with the cylinder 3 to fix the handle 8 to the lower inner portion of the cylinder 3.

[0040] When the handle 8 and the parts thereon are manually driven to move downward, so that the long needle 2 drives the tissue slice 16 thereon to be immersed in the liquid nitrogen, the tissue slice 16 overcomes the resistance of the liquid nitrogen and moves downward. The resistance acts on the tissue slice 16, increasing the risk of the tissue slice 16 loosening on the long needle 2. Therefore, when liquid nitrogen is poured into the cylinder 3 manually, the liquid nitrogen is only filled to the lower half of the cylinder 3, and then the handle 8 and the parts thereon are manually driven to move downward, so that the long needle 2 drives all the tissue slices 16 thereon to move to the inner upper half of the cylinder 3, and then the long rod 11 is manually pushed upward, and the long rod 11 drives the stopper 12 to move upward, and the stopper 12 drives the cover shell 10 to move upward. Since a large damping force is formed between the cover shell 10 and the cylinder 3, the cover shell 10 moves slowly upward, and the cover shell 10 and the stopper 12 push the liquid nitrogen to move slowly upward, so that the liquid nitrogen slowly immerses the tissue slice 16 from bottom to top, greatly reducing the impact force of the liquid nitrogen on the tissue slice 16, which is beneficial to improving the penetration stability. When all tissue slices 16 are immersed in liquid nitrogen, the long rod 11 is manually driven to move downward. Since the friction between the long rod 11 and the cover 1 5 is much smaller than the friction between the cover 2 10 and the cylinder 3, the cover 2 10 and the parts thereon remain stationary, and the long rod 11 drives the stopper 12 to move downward away from the cover 2 10, so that the stopper 12 stops covering the circular hole 1 94. After the device is placed in the liquid nitrogen tank, the liquid nitrogen in the circular hole 1 94, the cavity 1 92, the liquid permeable hole 91, the decompression Liquid flows between the filler 6, the second cavity 93 and the second circular hole 95 to balance the pressure inside and outside the cylinder 3. When in use, compared with directly immersing the tissue slice 16 from top to bottom in the liquid nitrogen, the friction between the cylinder 3 and the second cover 10 can make the liquid nitrogen slowly move upward to immerse the tissue slice 16, which greatly reduces the impact force on the tissue slice 16, is beneficial to improve the stability of the tissue slice 16 transferred on the long needle 2, and further avoids interference with the freezing preservation operation of the tissue slice 16.

[0041] Before putting the device into the liquid nitrogen tank, manually unscrew the handle 8 from the threaded column 7, then rotate the handle 8 180 degrees, and then insert the handle 8 from bottom to top into the lower inner part of the cylinder 3, and tighten the threaded cover 9 on the lower outer part of the cylinder 3. At this time, the state is as follows: Figure 7 As shown, the device is then placed in a liquid nitrogen tank for storage. When in use, the handle 8 can be fixed to the lower inner part of the cylinder 3 through the threaded cover 9, and then the storage operation is performed, which is beneficial to improving the space utilization rate of the liquid nitrogen tank.

[0042] Example 3

[0043] On the basis of Example 2, Figure 6 , Figure 7 and Fig. 9As shown, it also includes a reinforcement unit; the reinforcement unit is connected to the long rod 11; the reinforcement unit includes a spring 13, a linkage block 14 and a threaded block 15; the long rod 11 is fixedly connected with the spring 13; the linkage block 14 is fixedly connected to the spring 13, and the linkage block 14 is slidably connected to the long rod 11; two threaded blocks 15 are welded on the linkage block 14; another kind of thread is provided in the middle of the threaded cover 9; the pitch of the outer thread of the threaded cover 9 is greater than the pitch of the middle thread of the threaded cover 9; the threaded block 15 cooperates with the threaded cover 9.

[0044] The upper side edge of the threaded column 7 is chamfered to make it easier for the handle 8 to be screwed into the outer side of the threaded column 7 .

[0045] The inner edge of the lower side of the handle 8 is chamfered to make it easier for the handle 8 to be screwed into the outer side of the threaded column 7.

[0046] An anti-skid sleeve is arranged on the outer side of the threaded cover 9, which is helpful to improve the friction force and prevent the slipping phenomenon.

[0047] In the process of manually tightening the threaded cover 9 on the outside of the cylinder 3, the thread in the middle of the threaded cover 9 meshes with the thread block 15, and the threaded cover 9 is manually driven to rotate so that the threaded cover 9 is gradually screwed upward into the outside of the cylinder 3. During this process, the thread in the middle of the threaded cover 9 will rotate upward on the thread block 15, and the distance the threaded cover 9 rises after one rotation on the cylinder 3 is recorded as a, and the distance the threaded cover 9 rises after one rotation on the thread block 15 is recorded as b. Since the pitch of the thread on the outside of the threaded cover 9 is greater than the pitch of the thread in the middle of the threaded cover 9, a is greater than b. Therefore, when the threaded cover 9 is rotated upward on the cylinder 3 and the thread block 15 at the same time, the threaded cover 9 will force the thread block 15 to move downward, so that the threaded cover 9 is relatively 3 moves upward, which is equal to the distance that the threaded cover 9 moves upward relative to the threaded block 15. The threaded block 15 drives the linkage block 14 to move downward, and the linkage block 14 stretches the spring 13. The elastic force of the spring 13 acts on the long rod 11, so that the long rod 11 presses the block 12 on the cover 5 to prevent the block 12 from sliding freely and blocking the round hole 94, thereby avoiding interference with the liquid nitrogen exchange operation inside and outside the cylinder 3. When in use, when the threaded cover 9 is manually tightened on the cylinder 3, the threaded cover 9 will also drive the threaded block 15 to move downward, thereby pressing the block 12 on the cover 5 to prevent the block 12 from sliding freely and blocking the round hole 94, thereby avoiding interference with the liquid nitrogen exchange operation inside and outside the cylinder 3.

[0048] The technical principles of the embodiments of the present invention are described above in conjunction with specific embodiments. These descriptions are only for explaining the principles of the embodiments of the present invention and cannot be interpreted in any way as limiting the protection scope of the embodiments of the present invention. Based on the explanations herein, those skilled in the art can think of other specific implementation methods of the embodiments of the present invention without creative work, and these methods will fall within the protection scope of the embodiments of the present invention.

Claims

1. A cryogenic freezing device for preserving ovarian tissue, comprising a sieve (1); at least two long needles (2) are fixedly connected to the sieve (1); a cylinder (3) is arranged on the side of the sieve (1); the characteristics are: At least five groups of limit blocks (4) are fixedly connected to the cylinder (3), and each group of limit blocks (4) is provided with at least four limit blocks; the lengths of the limit blocks (4) are arranged to increase from top to bottom; the cylinder (3) is connected to a cover shell (5); a plurality of liquid permeable holes (91) are provided on the cover shell (5); and a pressure reducing filler (6) is provided on the inner side of the cover shell (5).

2. The low-temperature freezing device for ovarian tissue preservation according to claim 1, characterized in that: The sieve (1) consists of an upper cylindrical body and a lower conical body.

3. The low-temperature freezing device for ovarian tissue preservation according to claim 1, characterized in that: The upper opening of the cylinder (3) is in a trumpet shape.

4. The low-temperature freezing device for ovarian tissue preservation according to claim 1, characterized in that: It also includes a transfer unit; the transfer unit is connected to the sieve (1); the transfer unit includes a threaded column (7); the threaded column (7) is fixed to the sieve (1); a handle (8) is screwed onto the threaded column (7), and the handle (8) is in contact with the sieve (1).

5. A low temperature freezing device for ovarian tissue preservation according to claim 4, characterized in that: The auxiliary unit is also included; the cylinder (3) is connected to the auxiliary unit; the auxiliary unit includes a second cover shell (10); the cylinder (3) is connected to the second cover shell (10) in a damping sliding manner, and the first cover shell (5) is fixedly connected to the inner side of the second cover shell (10); the first cover shell (5) is connected to a long rod (11) in a damping sliding manner; the long rod (11) is fixedly connected to a stopper (12); the stopper (12) is in contact with the second cover shell (10); a contact is formed between the upper side of the first cover shell (5) and the second cover shell (10); A cavity (92) is formed, and the cavity (92) is connected to the corresponding liquid permeable hole (91); a cavity (93) is formed between the lower side of the cover shell (5) and the cover shell (10), and the cavity (93) is connected to the corresponding liquid permeable hole (91); a circular hole (94) is formed on the upper side of the cover shell (10), and the circular hole (94) is connected to the cavity (92); a circular hole (95) is formed on the lower side of the cover shell (10), and the circular hole (95) is connected to the cavity (93).

6. A low-temperature freezing device for ovarian tissue preservation according to claim 5, characterized in that: It also includes a threaded cover (9); the threaded cover (9) is fixedly connected to the handle (8); a thread is arranged on the lower part of the outer side of the cylinder (3); and the threaded cover (9) is matched with the cylinder (3).

7. A low-temperature freezing device for ovarian tissue preservation according to claim 6, characterized in that: The invention also comprises a reinforcement unit; the reinforcement unit is connected to the long rod (11); the reinforcement unit comprises a spring (13); the spring (13) is fixedly connected to the long rod (11); a linkage block (14) is fixedly connected to the spring (13), and the linkage block (14) is slidably connected to the long rod (11); at least two thread blocks (15) are fixedly connected to the linkage block (14); another thread is arranged in the middle of the threaded cover (9); the pitch of the thread on the outer side of the threaded cover (9) is greater than the pitch of the thread in the middle of the threaded cover (9); and the thread block (15) matches the threaded cover (9).

8. The low-temperature freezing device for ovarian tissue preservation according to claim 7, characterized in that: The upper side edge of the threaded column (7) is provided with a chamfer.

9. The low-temperature freezing device for ovarian tissue preservation according to claim 7, characterized in that: The inner edge of the lower side of the handle (8) is chamfered.

10. A low temperature freezing device for ovarian tissue preservation according to any one of claims 6 to 9, characterized in that: An anti-slip sleeve is arranged on the outer side of the threaded cover (9).

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