A beneficial bacterial strain culture fermentation device and fermentation method

Abstract

The present invention relates to the technical field of strain cultivation, specifically to a beneficial strain cultivation fermentation device and a fermentation method, comprising a fermentation unit, an aeration unit and an injection unit, wherein the fermentation unit comprises a fermentation tank, and the outer wall of the fermentation tank is fixedly installed with three forward-rotating aeration pipes with equal angle distribution and three reverse-rotating aeration pipes with equal angle distribution, the forward-rotating aeration pipe and the reverse-rotating aeration pipe are staggered and distributed in sequence up and down, and the angle between the forward-rotating aeration pipe and the reverse-rotating aeration pipe is 60 degrees. While the fermentation tank is aerated by the aeration unit, the vortex direction of the solution inside the fermentation tank is continuously and alternately changed, and each time the vortex direction of the solution inside the fermentation tank changes, a fluid counter-attack effect is formed, which is conducive to the diffusion of air inside the solution, ensuring the consistency of aerobic respiration of yeast, and more conducive to the cultivation of strains.

Description

Technical Field

[0001] The present invention relates to the technical field of strain cultivation, in particular to a beneficial strain cultivation and fermentation device and a fermentation method. Background Art

[0002] Strain improvement is the manipulation and modification of the metabolic capacity of microbial strains to enhance their application in biotechnology. For centuries, people have been cultivating specific microorganisms with unique genetic characteristics to improve the commercial properties of microbial strains, such as using specialized strains in the preparation of fermented foods and beverages (such as yogurt, cheese, and wine). Starting in the 19th century, the emergence of modern industrial microbiology made the application of pure culture and strain development possible. With the advancement of biochemical engineering and biomolecular manipulation technology, we can develop microbial strains in a more targeted manner.

[0003] According to a Chinese patent application with the publication number CN114752469A, a nitrite-reducing bacteria fermentation device and a fermentation method thereof are disclosed. While the nitrite-reducing bacteria pile inside the fermentation tank body is stirred, the air on the upper part of the fermentation tank body is sent into the interior of the nitrite-reducing bacteria pile. While increasing the contact area between the air and the nitrite-reducing bacteria pile, the blockage phenomenon that occurs when the bacteria air injection pipe is supplying air to the interior of the nitrite-reducing bacteria pile is effectively reduced, thereby improving the efficiency of nitrite-reducing bacteria fermentation. After using the above-mentioned bacteria fermentation device, it was found that during the operation of the stirring and air-guiding mechanism in the above-mentioned device, the stirring blade is easily rotated, which can cause irreversible damage to the cell shell of the yeast, affect the activity of the yeast, and be unfavorable for the reproduction of the yeast, resulting in the fermentation speed of the bacteria being affected. For this reason, we propose a beneficial strain culture fermentation device and a fermentation method to solve the above-mentioned technical problems. Summary of the Invention

[0004] The present invention provides the following technical solutions: a beneficial bacterial strain culture and fermentation device, comprising a fermentation unit, an aeration unit and an injection unit;

[0005] A fermentation unit, comprising a fermentation tank, wherein the outer wall of the fermentation tank is fixedly mounted with three forward-rotating aeration pipes and three reverse-rotating aeration pipes distributed at equal angles, wherein the forward-rotating aeration pipes and the reverse-rotating aeration pipes are staggered in sequence up and down, and the angle between the forward-rotating aeration pipes and the reverse-rotating aeration pipes is 60 degrees, the forward-rotating aeration pipes and the reverse-rotating aeration pipes are distributed at equal intervals in the rotation direction, and the rotation directions of the forward-rotating aeration pipes and the reverse-rotating aeration pipes are opposite, and a plurality of injection holes distributed at equal angles are opened through the lower portion of the outer wall of the fermentation tank;

[0006] An aeration unit, the aeration unit comprising a plurality of aeration cylinder sleeves fixedly mounted on the periphery of the fermentation tank, an aeration branch pipe and an air suction branch pipe fixedly mounted on the upper portion of the outer wall of each aeration cylinder sleeve, a first one-way valve fixedly mounted on the end of the aeration branch pipe, a second one-way valve fixedly mounted on the end of the air suction branch pipe, a piston slidably mounted inside the aeration cylinder sleeve, a push rod fixedly mounted on the top of the piston, the push rod movably extending to the periphery of the top of the aeration cylinder sleeve, a spring being disposed between the piston and the top wall of the aeration cylinder sleeve, and a cylindrical cam being rotatably disposed on the periphery of the fermentation tank;

[0007] The injection unit comprises a hyperbolic tube fixedly mounted on the bottom of the aeration cylinder sleeve, an injection tube fixedly mounted on the bottom of the hyperbolic tube, and a ball plug slidably mounted on the inner wall of the injection tube.

[0008] As a preferred solution of the present invention, the aeration branch pipe and the suction branch pipe are symmetrically distributed about the center of the aeration cylinder sleeve, the number of the first one-way valves corresponds to the total number of the forward rotation aeration pipes and the reverse rotation aeration pipes, and the positions of the first one-way valves correspond one-to-one to the positions of the forward rotation aeration pipes and the reverse rotation aeration pipes, and the ends of the first one-way valves away from the aeration branch pipes are fixedly connected to the ends of the forward rotation aeration pipes and the reverse rotation aeration pipes at the corresponding positions through flanges.

[0009] As a preferred solution of the present invention, a U-shaped wheel frame is fixedly installed on the top of the push rod, a guide wheel is rotatably installed inside the U-shaped wheel frame, the top of the outer wall of the guide wheel abuts against the bottom of the cylindrical cam, a rotating sleeve is fixedly installed on the inner wall of the cylindrical cam, and a plurality of pillars distributed at equal angles are fixedly installed on the outer wall of the fermentation unit, a turntable is fixedly installed on the upper part of the outer walls of the plurality of pillars, and the rotating sleeve is rotatably installed on the periphery of the turntable.

[0010] As a preferred solution of the present invention, a driven internal gear ring is fixedly installed on the bottom of the rotating sleeve, an AC motor is fixedly installed on one side of the outer wall of one of the pillars, a driving gear is fixedly installed on the output shaft of the AC motor, and the driving gear is meshed with the driven internal gear ring.

[0011] As a preferred solution of the present invention, two connecting plates distributed up and down are fixedly mounted on the outer wall of the aeration cylinder sleeve, and the aeration cylinder sleeve is fixedly mounted on the outer wall of the fermentation tank through the connecting plates.

[0012] As a preferred solution of the present invention, an air hole is opened through the bottom of the aeration cylinder sleeve, the interior of the hyperbolic tube and the interior of the aeration cylinder sleeve are connected through the air hole, the cross-section of the hyperbolic tube is hyperbolic, the end of the injection tube away from the hyperbolic tube is fixedly connected to the periphery of the opening of the injection hole, and the outer wall of the ball plug is slidably connected to the inner wall of the injection tube.

[0013] As a preferred solution of the present invention, a pressure relief valve is fixedly installed on the top of the fermentation tank.

[0014] As a preferred solution of the present invention, a sealing cover is detachably mounted at the top inlet of the fermenter, a discharge pipe is fixedly mounted at the bottom of the fermenter, and a shut-off valve is fixedly mounted at the end of the discharge pipe.

[0015] As a preferred solution of the present invention, a sealing ring is provided between the penetration point of the push rod and the aeration cylinder sleeve.

[0016] A fermentation method of a beneficial bacterial strain culture fermentation device comprises the following steps:

[0017] S1. Adding yeast solution and bacterial seed solution into the fermentation tank through the top inlet of the fermentation tank, and installing a sealing cover on the top of the fermentation tank to seal the top inlet of the fermentation tank;

[0018] S2. Adjust the temperature of the cultivation room to the cultivation temperature of the adapted strain;

[0019] S3. The cylindrical cam rotates to push the push rod downward, which drives the piston to move downward along the inner wall of the aeration cylinder. The second one-way valve opens, and external air is sucked into the aeration cylinder through the suction branch pipe. The downward movement of the piston causes the spring to stretch. When the cylindrical cam rotates and loses the downward thrust on the push rod, the spring's rebound force pushes the piston and push rod upward, and the air sucked into the aeration cylinder is transported through the aeration branch pipe and the first one-way valve to the inside of the forward and reverse aeration pipes, thereby aerating the solution inside the fermenter.

[0020] S4. The piston moves upward, causing negative pressure to form inside the hyperbolic tube and the injection tube. The ball moves along the inner wall of the injection tube toward one end of the hyperbolic tube, sucking the solution inside the fermenter into the injection tube. The piston moves downward along the aeration cylinder sleeve, injecting the air inside the aeration cylinder sleeve into the injection tube through the air holes and the hyperbolic tube. The pressure inside the injection tube increases, pushing the ball to move along the inner wall of the injection tube toward the injection hole, and injecting the solution sucked into the injection tube into the fermenter through the injection hole, accelerating mixing.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. In the present invention, while aerating the interior of the fermenter through the aeration unit, the vortex direction of the solution inside the fermenter is also continuously changed alternately. Each time the vortex direction of the solution inside the fermenter changes, a fluid counteraction effect is formed, which is beneficial to the diffusion of air inside the solution, ensuring the consistency of aerobic respiration of yeast, and is more conducive to the cultivation of strains.

[0023] 2. In the present invention, during the work of the aeration unit, the solution inside the fermentation tank is sucked into the interior of the injection tube through the injection hole, and then the solution sucked into the interior of the injection tube is injected into the interior of the fermentation tank through the injection hole, causing the solution inside the fermentation tank to flow, which is beneficial to the uniform diffusion of yeast in the bacterial liquid, thereby bringing better fermentation effect.

[0024] 3. In the present invention, the internal volumes of the aeration cylinder sleeve and the injection tube are adapted to each other, that is, when the piston moves to the bottom dead center position, the ball plug is pushed to move along the inner wall of the injection tube to a position close to the injection hole, so that the ball plug will not enter the interior of the fermenter through the injection hole. By pushing the ball plug along the inner wall of the injection tube, the solution attached to the inner wall of the injection tube can be scraped off and injected into the interior of the fermenter together, avoiding solution residue on the inner wall of the injection tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structure of the present invention;

[0026] Figure 2 For the present invention Figure 1 Schematic diagram of the local structure;

[0027] Figure 3 Schematic diagram of the structure of the fermentation unit of the present invention;

[0028] Figure 4 Schematic diagram of the structure of the aeration unit in the present invention;

[0029] Figure 5 Schematic diagram of the cross-sectional structure of the aeration cylinder sleeve in the present invention;

[0030] Figure 6 Schematic diagram of the cross-sectional structure of the injection tube in the present invention;

[0031] Figure 7 Schematic diagram of the top cross-sectional structure of the fermentation tank of the present invention;

[0032] Figure 8 Schematic diagram of the expanded structure of the cylindrical cam in the present invention;

[0033] Figure 9 It is a front structural schematic diagram of the present invention.

[0034] In the figure: 100, fermentation unit; 101, fermentation tank; 102, forward rotation aeration pipe; 103, reverse rotation aeration pipe; 104, injection hole; 105, turntable; 106, support; 107, pressure relief valve; 108, discharge pipe; 109, shut-off valve; 1010, sealing cover; 200, aeration unit; 201, aeration cylinder sleeve; 202, aeration branch pipe; 203, first non-return valve; 204, suction branch pipe; 20 5. Second one-way valve; 206. Piston; 207. Push rod; 208. Spring; 209. U-shaped wheel frame; 2010. Guide wheel; 2011. Cylindrical cam; 2012. Driven internal gear ring; 2013. Rotating sleeve; 2014. AC motor; 2015. Driving gear; 2016. Connecting plate; 300. Injection unit; 301. Hyperbolic tube; 302. Air hole; 304. Injection tube; 305. Ball plunger. DETAILED DESCRIPTION

[0035] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] See also Figures 1-9 The technical solution provided by the present invention specifically includes the following embodiments:

[0037] Embodiment 1: A beneficial strain culture and fermentation device includes a fermentation unit 100, an aeration unit 200 and an injection unit 300. The fermentation unit 100 includes a fermentation tank 101. The outer wall of the fermentation tank 101 is fixedly mounted with three forward-rotating aeration pipes 102 distributed at equal angles and three reverse-rotating aeration pipes 103 distributed at equal angles. The forward-rotating aeration pipes 102 and the reverse-rotating aeration pipes 103 are staggered in sequence up and down, and the angle between the forward-rotating aeration pipes 102 and the reverse-rotating aeration pipes 103 is 60 degrees. The forward-rotating aeration pipes 102 and the reverse-rotating aeration pipes 103 are equidistantly distributed in the direction of rotation, and the rotation directions of the forward-rotating aeration pipes 102 and the reverse-rotating aeration pipes 103 are opposite. A plurality of injection holes 104 distributed at equal angles are opened through the lower part of the outer wall of the fermentation tank 101.

[0038] The aeration unit 200 includes a plurality of aeration cylinder sleeves 201 fixedly mounted on the periphery of the fermentation tank 101. Two upper and lower connecting plates 2016 are fixedly mounted on the outer wall of the aeration cylinder sleeve 201. The aeration cylinder sleeve 201 is fixedly mounted on the outer wall of the fermentation tank 101 through the connecting plates 2016. An aeration branch pipe 202 and an air suction branch pipe 204 are fixedly mounted on the upper part of the outer wall of the aeration cylinder sleeve 201. A first one-way valve 203 is fixedly mounted on the end of the aeration branch pipe 202, and a second one-way valve 205 is fixedly mounted on the end of the air suction branch pipe 204. A piston 206 is slidably mounted inside the aeration cylinder sleeve 201, and a push rod 207 is fixedly mounted on the top of the piston 206. The push rod 207 movably extends to the aeration cylinder sleeve 201. On the outer periphery of the top, a sealing ring is provided between the push rod 207 and the point where the aeration cylinder sleeve 201 passes through, a spring 208 is provided between the piston 206 and the top wall of the aeration cylinder sleeve 201, a cylindrical cam 2011 is provided for the rotation of the outer periphery of the fermentation tank 101, the aeration branch pipe 202 and the suction branch pipe 204 are symmetrically distributed about the center of the aeration cylinder sleeve 201, the number of the first one-way valves 203 corresponds to the total number of the forward rotation aeration pipes 102 and the reverse rotation aeration pipes 103, and the positions of the first one-way valves 203 correspond one-to-one to the positions of the forward rotation aeration pipes 102 and the reverse rotation aeration pipes 103, and the ends of the first one-way valves 203 away from the aeration branch pipes 202 are fixedly connected to the ends of the forward rotation aeration pipes 102 and the reverse rotation aeration pipes 103 at the corresponding positions through flanges;

[0039] A rotating sleeve 2013 is fixedly mounted on the inner wall of the cylindrical cam 2011. A plurality of equally angularly distributed pillars 106 are fixedly mounted on the outer wall of the fermentation unit 100. A turntable 105 is fixedly mounted on the upper portion of the outer walls of the plurality of pillars 106. The rotating sleeve 2013 is rotatably mounted on the periphery of the turntable 105. A driven internal gear ring 2012 is fixedly mounted on the bottom of the rotating sleeve 2013. An AC motor 2014 is fixedly mounted on one side of the outer wall of one of the pillars 106. A driving gear 215 is fixedly mounted on the output shaft of the AC motor 2014. The driving gear 215 meshes with the driven internal gear ring 2012.

[0040] Specifically, in this embodiment, the output shaft of the AC motor 2014 drives the driving gear 2015 to rotate. The driving gear 2015 rotates and drives the rotating sleeve 2013 and the cylindrical cam 2011 to rotate around the outer wall of the turntable 105 through the meshing action with the driven inner gear ring 2012. During the rotation of the cylindrical cam 2011, the three push rods 207 corresponding to the positions of the three forward-rotating aeration tubes 102 and the three push rods 207 corresponding to the positions of the three reverse-rotating aeration tubes 103 are pushed downward in turn. The push rods 207 are pushed downward to drive the piston 206 slides downward along the inner wall of the aeration cylinder sleeve 201, causing the spring 208 to stretch and store force. It should be noted that the number and position of the convex and concave portions at the bottom of the cylindrical cam 2011 in this device correspond one-to-one with the number and position of the push rods 207. When the three push rods 207 corresponding to the positions of the forward-rotating aeration pipes 102 are pushed downward, the cylindrical cam 2011 will not exert downward pressure on the remaining three push rods 207. Therefore, the remaining push rods 207 corresponding to the positions of the three reverse-rotating aeration pipes 103 will reset upward under the action of the rebound force of the spring 208, and as the cylindrical cam 2011 is pushed downward, the push rods 207 corresponding to the positions of the reverse-rotating aeration pipes 103 will reset upward under the action of the rebound force of the spring 208. The column cam 2011 continues to rotate, pushing the three push rods 207 corresponding to the positions of the three reverse-rotating aeration tubes 103 downward. Similarly, the three push rods 207 corresponding to the positions of the three forward-rotating aeration tubes 102 are reset upward under the action of the rebound force of the spring 208. That is to say, the push rods 207 corresponding to the positions of the forward-rotating aeration tubes 102 and the reverse-rotating aeration tubes 103 in this device are always moving in an up-and-down manner, further causing the pistons 206 connected to the push rods 207 to move together. When the pistons 206 corresponding to the positions of the forward-rotating aeration tubes 102 move downward, the pistons 206 connected to the push rods 207 are reset upward. , the outside air is sucked into the aeration cylinder liner 201 through the second one-way valve 205 and the suction branch pipe 204, and as the piston 206 returns to its original position, the piston 206 compresses the air sucked into the aeration cylinder liner 201, and the compressed air is transported to the inside of the positive rotation aeration pipe 102 through the aeration branch pipe 202 and the first one-way valve 203, and then transported to the inside of the fermentation tank 101 through the positive rotation aeration pipe 102, aerating the solution in the fermentation tank 101, and due to the blowing effect of the air, the solution in the fermentation tank 101 produces Figure 7The vortex effect in the direction of the arrow shown, when the cylindrical cam 2011 presses down the push rod 207 corresponding to the position of the reverse rotation aeration pipe 103, the push rod 207 drives the piston 206 connected thereto to move downward along the aeration cylinder sleeve 201, and the external air is sucked into the interior of the aeration cylinder sleeve 201 through the second one-way valve 205 and the suction branch pipe 204, and as the piston 206 returns upward, the piston 206 compresses the air sucked into the interior of the aeration cylinder sleeve 201, and the compressed air is transported to the interior of the reverse rotation aeration pipe 103 through the aeration branch pipe 202 and the first one-way valve 203, and then transported to the interior of the fermentation tank 101 through the reverse rotation aeration pipe 103, so as to aerate the solution inside the fermentation tank 101 again, and due to the blowing effect of the air, the solution inside the fermentation tank 101 produces Figure 7 The vortex effect in the opposite direction of the arrows shown, under the continuous rotation of the cylindrical cam 2011, aerates the interior of the fermenter 101 while also alternating the vortex direction of the solution inside the fermenter 101. Each time the vortex direction of the solution inside the fermenter 101 changes, a fluid counteraction effect is formed, which is beneficial to the diffusion of air inside the solution, ensuring the consistency of the aerobic respiration of the yeast, and is more conducive to the cultivation of the strain.

[0041] Embodiment 2: The injection unit 300 includes a hyperbolic tube 301 fixedly mounted on the bottom of the aeration cylinder sleeve 201, an injection tube 304 fixedly mounted on the bottom of the hyperbolic tube 301, a ball plug 305 slidably mounted on the inner wall of the injection tube 304, an air hole 302 is formed through the bottom of the aeration cylinder sleeve 201, the interior of the hyperbolic tube 301 and the interior of the aeration cylinder sleeve 201 are connected through the air hole 302, the cross section of the hyperbolic tube 301 is hyperbolic, one end of the injection tube 304 away from the hyperbolic tube 301 is fixedly connected to the periphery of the opening of the injection hole 104, and the outer wall of the ball plug 305 is slidably connected to the inner wall of the injection tube 304;

[0042] Specifically, in this embodiment, when the piston 206 is reset upward, the air inside the injection tube 304 and the hyperbolic tube 301 is sucked into the aeration cylinder sleeve 201, resulting in negative pressure inside the injection tube 304, and the ball plug 305 is adsorbed along the inner wall of the injection tube 304 toward one end of the aeration cylinder sleeve 201. The ball plug 305 moves along the inner wall of the injection tube 304 toward one end of the aeration cylinder sleeve 201, and the solution inside the fermentation tank 101 is sucked into the injection tube 304 through the injection hole 104. As the piston 206 moves downward, the air inside the aeration cylinder sleeve 201 is injected into the injection tube 304 again through the hyperbolic tube 301. Under the action of air pressure, the ball plug 305 is pushed toward the injection hole 104, and the solution sucked into the injection tube 304 is passed through the injection hole 104. 4 is injected into the interior of the fermentation tank 101, causing the solution inside the fermentation tank 101 to flow, which is conducive to the uniform diffusion of yeast in the bacterial liquid, thereby bringing about a better fermentation effect. It should be supplemented that the internal volumes of the aeration cylinder sleeve 201 and the injection tube 304 in the present device are adapted to each other. That is, when the piston 206 moves to the bottom dead center position, it pushes the ball plug 305 to move along the inner wall of the injection tube 304 to a position close to the injection hole 104, so that the ball plug 305 will not enter the interior of the fermentation tank 101 through the injection hole 104. Instead, by pushing the ball plug 305 to move along the inner wall of the injection tube 304, the solution attached to the inner wall of the injection tube 304 can be scraped off and injected into the interior of the fermentation tank 101 together, avoiding solution residue on the inner wall of the injection tube 304.

[0043] Embodiment 3: A U-shaped wheel frame 209 is fixedly installed on the top of the push rod 207, and a guide wheel 2010 is rotatably installed inside the U-shaped wheel frame 209, and the top of the outer wall of the guide wheel 2010 abuts against the bottom of the cylindrical cam 2011; by setting the U-shaped wheel frame 209, the guide wheel 2010 is rotatably installed on the top of the push rod 207, and through the rolling action between the guide wheel 2010 and the bottom of the cylindrical cam 2011, the convex surface of the bottom of the cylindrical cam 2011 does not directly rub against the top of the push rod 207, thereby ensuring the smoothness of the rotation of the cylindrical cam 2011, and at the same time avoiding the loss of the cylindrical cam 2011 and the push rod 207 due to friction, thereby improving the durability of the device.

[0044] A pressure relief valve 107 is fixedly installed on the top of the fermentation tank 101; as the interior of the fermentation tank 101 is continuously aerated, the air pressure inside the fermentation tank 101 will increase. When the air pressure value inside the fermentation tank 101 exceeds the pressure relief value of the pressure relief valve 107, the air is discharged to the outside through the pressure relief valve 107, so that the air pressure inside the fermentation tank 101 is maintained within a reasonable range, thereby preventing high pressure from affecting the fermentation cultivation of the strain.

[0045] A sealing cover 1010 is detachably mounted at the top inlet of the fermentation tank 101, a discharge pipe 108 is fixedly mounted at the bottom of the fermentation tank 101, and a shut-off valve 109 is fixedly mounted at the end of the discharge pipe 108; yeast liquid and bacterial liquid are added into the interior of the fermentation tank 101 through the top inlet of the fermentation tank 101, the sealing cover 1010 is mounted on the top of the fermentation tank 101, the top inlet of the fermentation tank 101 is sealed, and then fermentation is carried out. After the fermentation is completed, the shut-off valve 109 is opened to discharge the fermented bacterial liquid inside the fermentation tank 101 through the discharge pipe 108, thereby improving the convenience of use.

[0046] A fermentation method of a beneficial bacterial strain culture fermentation device comprises the following steps:

[0047] S1. Add the yeast solution and the bacterial seed solution into the fermentation tank 101 through the top inlet of the fermentation tank 101, and install the sealing cover 1010 on the top of the fermentation tank 101 to seal the top inlet of the fermentation tank 101;

[0048] S2. Adjust the temperature of the cultivation room to the cultivation temperature of the adapted strain;

[0049] S3. The cylindrical cam 2011 rotates to push the push rod 207 downward. The push rod 207 drives the piston 206 to move downward along the inner wall of the aeration cylinder liner 201. The second one-way valve 205 opens, and external air is sucked into the interior of the aeration cylinder liner 201 through the suction branch pipe 204. The downward movement of the piston 206 causes the spring 208 to be stretched. When the cylindrical cam 2011 rotates and loses the downward thrust on the push rod 207, the rebound force of the spring 208 pushes the piston 206 and the push rod 207 upward. The air sucked into the interior of the aeration cylinder liner 201 is transported through the aeration branch pipe 202 and the first one-way valve 203 to the interior of the forward-rotating aeration pipe 102 and the reverse-rotating aeration pipe 103, thereby aerating the solution inside the fermenter 101.

[0050] S4. The piston 206 moves upward, causing negative pressure to be generated inside the hyperbolic tube 301 and the injection tube 304. The ball plug 305 moves along the inner wall of the injection tube 304 toward one end of the hyperbolic tube 301, sucking the solution inside the fermentation tank 101 into the injection tube 304. The piston 206 moves downward along the aeration cylinder sleeve 201, injecting the air inside the aeration cylinder sleeve 201 into the injection tube 304 through the air hole 302 and the hyperbolic tube 301. The pressure inside the injection tube 304 increases, pushing the ball plug 305 to move along the inner wall of the injection tube 304 toward the injection hole 104, and injecting the solution sucked into the injection tube 304 into the fermentation tank 101 through the injection hole 104, thereby accelerating mixing.

[0051] In the present embodiment, a beneficial strain culture and fermentation device is operated by adding yeast solution and bacterial seed solution into the fermentation tank 101 through the top inlet of the fermentation tank 101. The top inlet of the fermentation tank 101 is sealed by a sealing cover 1010 installed on the top of the fermentation tank 101, and the temperature of the cultivation room is adjusted to a temperature suitable for cultivating the strain.

[0052] After the above process is completed, the AC motor 2014 is started, and the output shaft of the AC motor 2014 drives the driving gear 2015 to rotate. The driving gear 2015 rotates and drives the rotating sleeve 2013 and the cylindrical cam 2011 to rotate around the outer wall of the turntable 105 through the meshing action with the driven inner gear ring 2012. During the rotation of the cylindrical cam 2011, the three push rods 207 corresponding to the positions of the three forward-rotating aeration pipes 102 and the three push rods 207 corresponding to the positions of the three reverse-rotating aeration pipes 103 are pushed downward alternately. 7 is pushed downward, driving the piston 206 to slide downward along the inner wall of the aeration cylinder sleeve 201, causing the spring 208 to stretch and store force. It should be noted that the number and position of the convex and concave portions at the bottom of the cylindrical cam 2011 in this device correspond one-to-one with the number and position of the ejector rods 207. When the three ejector rods 207 corresponding to the positions of the forward-rotating aeration pipes 102 are pushed downward, the cylindrical cam 2011 will not exert downward pressure on the remaining three ejector rods 207. Therefore, the remaining ejector rods 207 corresponding to the positions of the three reverse-rotating aeration pipes 103 will move upward under the rebound force of the spring 208. The three push rods 207 corresponding to the positions of the three reverse-rotating aeration pipes 103 are pushed downwards as the cylindrical cam 2011 continues to rotate. Similarly, the three push rods 207 corresponding to the positions of the three forward-rotating aeration pipes 102 are reset upwards under the action of the rebound force of the spring 208. That is to say, the push rods 207 corresponding to the positions of the forward-rotating aeration pipe 102 and the reverse-rotating aeration pipe 103 in the device are always in an up-and-down movement, further causing the pistons 206 connected to the push rods 207 to move together. When the pistons 206 corresponding to the positions of the forward-rotating aeration pipe 102 are reset, the pistons 206 connected to the push rods 207 are reset. When the piston 206 moves downward, the external air is sucked into the aeration cylinder liner 201 through the second one-way valve 205 and the suction branch pipe 204. As the piston 206 returns upward, the piston 206 compresses the air sucked into the aeration cylinder liner 201, and the compressed air is transported to the inside of the positive rotation aeration pipe 102 through the aeration branch pipe 202 and the first one-way valve 203. Then, the air is transported to the inside of the fermentation tank 101 through the positive rotation aeration pipe 102, aerating the solution inside the fermentation tank 101. Due to the blowing effect of the air, the solution inside the fermentation tank 101 produces the following Figure 7The vortex effect in the direction of the arrow shown, when the cylindrical cam 2011 presses down the push rod 207 corresponding to the position of the reverse rotation aeration pipe 103, the push rod 207 drives the piston 206 connected thereto to move downward along the aeration cylinder sleeve 201, and the external air is sucked into the interior of the aeration cylinder sleeve 201 through the second one-way valve 205 and the suction branch pipe 204, and as the piston 206 returns upward, the piston 206 compresses the air sucked into the interior of the aeration cylinder sleeve 201, and the compressed air is transported to the interior of the reverse rotation aeration pipe 103 through the aeration branch pipe 202 and the first one-way valve 203, and then transported to the interior of the fermentation tank 101 through the reverse rotation aeration pipe 103, so as to aerate the solution inside the fermentation tank 101 again, and due to the blowing effect of the air, the solution inside the fermentation tank 101 produces Figure 7 The vortex effect in the opposite directions of the arrows shown, so that under the continuous rotation of the cylindrical cam 2011, while aerating the interior of the fermenter 101, also causes the vortex direction of the solution inside the fermenter 101 to continuously and alternately change. Each change in the vortex direction of the solution inside the fermenter 101 will form a fluid counteraction effect, which is conducive to the diffusion of air inside the solution, ensuring the consistency of aerobic respiration of the yeast, and is more conducive to the cultivation of the strain.

[0053] At the same time, when the piston 206 is reset upward, the air inside the injection tube 304 and the hyperbolic tube 301 is sucked into the aeration cylinder sleeve 201, resulting in negative pressure inside the injection tube 304, and the ball plug 305 is adsorbed along the inner wall of the injection tube 304 toward one end of the aeration cylinder sleeve 201. The ball plug 305 moves along the inner wall of the injection tube 304 toward one end of the aeration cylinder sleeve 201, and the solution inside the fermentation tank 101 is sucked into the injection tube 304 through the injection hole 104. As the piston 206 moves downward, the air inside the aeration cylinder sleeve 201 is injected into the injection tube 304 again through the hyperbolic tube 301. Under the action of air pressure, the ball plug 305 is pushed toward the injection hole 104, and the solution sucked into the injection tube 304 is injected into the fermentation tank 101 through the injection hole 104, causing the solution inside the fermentation tank 101 to flow, which is conducive to the uniform diffusion of yeast in the seed liquid, thereby bringing better fermentation effect.

[0054] While the embodiments of the present invention have been shown and described, it will be apparent to those skilled in the art that various changes, modifications, substitutions, and alterations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A beneficial bacterial strain culture and fermentation device, characterized in that: It includes a fermentation unit (100), an aeration unit (200) and an injection unit (300); A fermentation unit (100), the fermentation unit (100) comprising a fermentation tank (101), the outer wall of the fermentation tank (101) being fixedly mounted with three forward-rotating aeration pipes (102) distributed at equal angles and three reverse-rotating aeration pipes (103) distributed at equal angles, the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103) being staggered in sequence up and down, and the angle between the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103) being 60 degrees, the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103) being distributed at equal intervals in terms of rotation direction, and the rotation directions of the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103) being opposite, and a plurality of injection holes (104) distributed at equal angles are provided through the lower portion of the outer wall of the fermentation tank (101); An aeration unit (200), the aeration unit (200) comprising a plurality of aeration cylinder sleeves (201) fixedly mounted on the periphery of a fermentation tank (101), an aeration branch pipe (202) and an air suction branch pipe (204) fixedly mounted on the upper portion of the outer wall of each of the aeration cylinder sleeves (201), a first non-return valve (203) fixedly mounted on the end of the aeration branch pipe (202), a second non-return valve (205) fixedly mounted on the end of the air suction branch pipe (204), a piston (206) slidably mounted inside the aeration cylinder sleeve (201), a push rod (207) fixedly mounted on the top of the piston (206), the push rod (207) movably extending to the periphery of the top of the aeration cylinder sleeve (201), a spring (208) being arranged between the piston (206) and the top wall of the aeration cylinder sleeve (201), and a cylindrical cam (2011) being rotatably arranged on the periphery of the fermentation tank (101); An injection unit (300) comprises a hyperbolic tube (301) fixedly mounted on the bottom of an aeration cylinder sleeve (201), an injection tube (304) fixedly mounted on the bottom of the hyperbolic tube (301), and a ball plug (305) slidably mounted on the inner wall of the injection tube (304).

2. The beneficial strain culture and fermentation device according to claim 1, characterized in that: The aeration branch pipes (202) and the air suction branch pipes (204) are symmetrically distributed about the center of the aeration cylinder sleeve (201); the number of the first one-way valves (203) corresponds to the total number of the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103); the positions of the first one-way valves (203) correspond one-to-one to the positions of the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103); and the ends of the first one-way valves (203) away from the aeration branch pipes (202) are fixedly connected to the ends of the forward-rotating aeration pipes (102) and the reverse-rotating aeration pipes (103) at corresponding positions via flanges.

3. A beneficial strain culture and fermentation device according to claim 2, characterized in that: A U-shaped wheel frame (209) is fixedly mounted on the top of the push rod (207), a guide wheel (2010) is rotatably mounted inside the U-shaped wheel frame (209), the top of the outer wall of the guide wheel (2010) abuts against the bottom of the cylindrical cam (211), a rotating sleeve (213) is fixedly mounted on the inner wall of the cylindrical cam (2011), a plurality of pillars (106) distributed at equal angles are fixedly mounted on the outer wall of the fermentation unit (100), a turntable (105) is fixedly mounted on the upper parts of the outer walls of the plurality of pillars (106), and the rotating sleeve (2013) is rotatably mounted on the periphery of the turntable (105).

4. A beneficial strain culture and fermentation device according to claim 3, characterized in that: A driven internal gear ring (2012) is fixedly mounted on the bottom of the rotating sleeve (2013), an AC motor (2014) is fixedly mounted on one side of the outer wall of one of the pillars (106), a driving gear (2015) is fixedly mounted on the output shaft of the AC motor (2014), and the driving gear (2015) is meshed with the driven internal gear ring (2012).

5. A beneficial strain culture and fermentation device according to claim 4, characterized in that: Two connecting plates (2016) distributed up and down are fixedly mounted on the outer wall of the aeration cylinder sleeve (201), and the aeration cylinder sleeve (201) is fixedly mounted on the outer wall of the fermentation tank (101) via the connecting plates (2016).

6. A beneficial strain culture and fermentation device according to claim 5, characterized in that: An air hole (302) is formed through the bottom of the aeration cylinder sleeve (201); the interior of the hyperbolic tube (301) and the interior of the aeration cylinder sleeve (201) are connected through the air hole (302); the cross section of the hyperbolic tube (301) is in a hyperbolic shape; one end of the injection tube (304) away from the hyperbolic tube (301) is fixedly connected to the periphery of the opening of the injection hole (104); and the outer wall of the ball plug (305) is slidably connected to the inner wall of the injection tube (304).

7. A beneficial strain culture and fermentation device according to claim 6, characterized in that: A pressure relief valve (107) is fixedly installed on the top of the fermentation tank (101).

8. The beneficial strain culture and fermentation device according to claim 7, characterized in that: A sealing cover (1010) is detachably mounted at the top inlet of the fermentation tank (101), a discharge pipe (108) is fixedly mounted at the bottom of the fermentation tank (101), and a shut-off valve (109) is fixedly mounted at the end of the discharge pipe (108).

9. A beneficial strain culture and fermentation device according to claim 8, characterized in that: A sealing ring is provided between the penetration point of the push rod (207) and the aeration cylinder sleeve (201).

10. The fermentation method of the beneficial strain culture fermentation device according to claim 9, characterized in that: The usage steps include the following: S1, adding yeast liquid and bacterial seed liquid into the interior of the fermentation tank (101) through the top inlet of the fermentation tank (101), installing a sealing cover (1010) on the top of the fermentation tank (101), and sealing the top inlet of the fermentation tank (101); S2. Adjust the temperature of the cultivation room to the cultivation temperature of the adapted strain; S3, the cylindrical cam (2011) is rotated to push the push rod (207) downward, and the push rod (207) drives the piston (206) to move downward along the inner wall of the aeration cylinder sleeve (201), and the second one-way valve (205) is opened to suck external air into the interior of the aeration cylinder sleeve (201) through the suction branch pipe (204), and the piston (206) moves downward, causing the spring (208) to be stretched. When the cylindrical cam (2011) rotates and loses the downward thrust on the push rod (207), the rebound force of the spring (208) pushes the piston (206) and the push rod (207) upward, and the air sucked into the interior of the aeration cylinder sleeve (201) is transported to the interior of the forward-rotating aeration pipe (102) and the reverse-rotating aeration pipe (103) through the aeration branch pipe (202) and the first one-way valve (203), so as to aerate the solution inside the fermentation tank (101); S4. The piston (206) moves upward, causing negative pressure to be generated inside the hyperbolic tube (301) and the injection tube (304). The ball plug (305) moves along the inner wall of the injection tube (304) toward one end of the hyperbolic tube (301), sucking the solution inside the fermentation tank (101) into the injection tube (304). The piston (206) moves downward along the aeration cylinder sleeve (201), injecting the air inside the aeration cylinder sleeve (201) into the injection tube (304) through the air hole (302) and the hyperbolic tube (301). The pressure inside the injection tube (304) increases, pushing the ball plug (305) to move along the inner wall of the injection tube (304) toward the injection hole (104), injecting the solution sucked into the injection tube (304) into the fermentation tank (101) through the injection hole (104), thereby accelerating mixing.

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