Automatic chromatographic system with multiple columns connected in parallel

Abstract

The invention discloses a multi-column parallel automatic chromatographic system, which is provided with more than two chromatographic columns connected in parallel, and all the chromatographic columns share a mobile phase device, a detection device and other devices, and has the following advantages: (1) the chromatographic columns connected in parallel share a set of software control system, so that the simultaneous operation of the plurality of chromatographic columns is realized; the product analysis, separation and purification time is shortened, and the labor cost is reduced; (2) the chromatographic columns connected in parallel share a mobile phase device, a detector device, a fraction collector and other devices, and equipment is reduced; and (3) common parts are used, so that the plant area is reduced, the load-bearing requirement is reduced, and the load-bearing and area requirements of the plant are reduced, thereby reducing the construction cost.

Description

Technical Field

[0001] The invention relates to a chromatography system, in particular to an automated chromatography system with multiple columns connected in parallel. Background Art

[0002] Chromatographic system is the most effective method for analyzing, separating and purifying drugs at present, but it is limited by the amount of chromatographic sample loading, running time, etc., and the production capacity is limited, and it is only used in high value-added products. The single-cycle chromatographic system commonly used on the market currently adopts a single-column scheme, and performs equilibrium-loading-elution-washing column operation in a cycle. The chromatographic system cannot realize automatic continuous operation, and there is only one chromatographic column, and the purification efficiency is low, which cannot meet the increasing demand for drug quality and the need to reduce production costs. CN03146864.0 discloses another continuous chromatographic system, which adopts an array chromatographic column, and the solvent is evenly fed into each column. The feeding mode of the simulated annular chromatography is switched in sequence, periodically, and evenly to feed the chromatographic column. The eluted product is collected using a special product collector, and is respectively sent to each product storage container, and the sample is collected after detection. The chromatographic column system adopts a single mobile phase, and cannot purify multi-mobile phase samples, and the chromatographic column cannot be regenerated and reused. The sample collection is not equipped with a monitoring system, and there is a risk. Summary of the invention

[0003] In view of this, in order to overcome the shortcomings of the prior art, the present invention provides a multi-column parallel automated chromatography system, which is provided with more than two parallel chromatographic columns, all of which share other devices such as a mobile phase device and a detection device, and the chromatographic columns are synchronized in one system, which greatly reduces the time for product analysis, separation and purification. While improving output efficiency, it also reduces manpower, equipment and plant costs.

[0004] To achieve the above object, the present invention provides a solution: an automated chromatography system with multiple columns in parallel, comprising a sample tank 1, a mobile phase device 2, a multi-way solvent selection valve 3, a chromatography pump 4, a chromatography column device 5, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9 and a connecting pipeline; the mobile phase device comprises at least two mobile phase storage tanks arranged in parallel, the number of the chromatography pumps is greater than or equal to 2 and they are arranged in parallel, the chromatography column device comprises at least two identical chromatography columns arranged in parallel, the detector device comprises at least one detector, and the detector device is arranged in parallel with the waste liquid tank;

[0005] After the sample tank 1 and the mobile phase device 2 are connected in parallel, they are connected to the multi-way solvent selection valve 3 through a connecting pipe, and the multi-way solvent selection valve 3 is connected to the chromatographic pump through a connecting pipe; the chromatographic pump is connected to the chromatographic column device 5 through a connecting pipe, and the chromatographic column device 5 is connected to the post-column valve 6 through a connecting pipe, and the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through a connecting pipe, and the detector device 7 is connected to the fraction collector 8 through a connecting pipe.

[0006] When the system is running, the liquid in the sample tank 1 and the mobile phase device 2 flows to the chromatographic pump through the multi-way solvent selection valve 3; the liquid processed by the chromatographic column selects the detector device 7 or the waste liquid tank 9 through the post-column valve 6, and the liquid passing through the detector device 7 is collected by the fraction collector 8 connected in series with the detector device.

[0007] The present invention provides another solution: an automated chromatography system with multiple columns in parallel, comprising a sample tank 1, a mobile phase device 2, a chromatography pump 4, a chromatography column device 5, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, a pre-column valve 10 and connecting pipes;

[0008] The invention is characterized in that the mobile phase device comprises at least two mobile phase storage tanks arranged in parallel,

[0009] The number of chromatographic pumps is greater than or equal to 3,

[0010] The chromatographic column device comprises at least two identical chromatographic columns arranged in parallel,

[0011] The detector device comprises at least one detector,

[0012] The detector device is arranged in parallel with the waste liquid tank;

[0013] Among them, the sample tank 1 is connected to the chromatographic pump 4 through a connecting pipe to form a sample delivery passage, the mobile phase storage tanks arranged in parallel are respectively connected to the chromatographic pump 4 through connecting pipes, and then connected to form a mobile phase delivery passage through connecting pipes, the sample delivery passage and the mobile phase delivery passage are respectively connected to the pre-column valve 10 through connecting pipes, the pre-column valve 10 is connected to the chromatographic column device 5 through a connecting pipe, the chromatographic column device 5 is connected to the post-column valve 6 through a connecting pipe, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through a connecting pipe, and the detector device 7 is connected to the fraction collector 8 through a connecting pipe.

[0014] When the system is running, the liquid in the sample delivery path and the mobile phase delivery path is selected to flow to the chromatographic column device 5 through the pre-column valve 10; the liquid processed by the chromatographic column is selected to flow to the detector device 7 or the waste liquid tank 9 through the post-column valve 6, and the liquid passing through the detector device (7) is collected by the fraction collector 8 connected in series with the detector device.

[0015] Preferably, in the first embodiment, the chromatography system further comprises a pre-column valve 10, which is arranged before the chromatography pump or the chromatography column, and the liquid selectively flows through the pre-column valve to the chromatography pump to reach the corresponding chromatography column, or directly reaches the corresponding chromatography column.

[0016] Preferably, in both schemes, when the detector device includes two or more detectors, the detectors are identical and are arranged in parallel; when the elution collection time is long, multiple detectors can be started simultaneously to save system and software working time.

[0017] Preferably, the detectors of the two schemes are selected from ultraviolet detectors, conductivity detectors, diode array detectors, evaporative light scattering detectors, and mass spectrometry detectors.

[0018] Preferably, in the gradient mode in both schemes, the chromatographic pump is selected from one or both of a common pump and a high-precision pump, so as to reduce the cost while meeting the elution requirements and ensuring the stability of the peak time.

[0019] Preferably, in the gradient mode in the first solution, the automated chromatography system further comprises a proportional valve 11 , and the gradient elution can be controlled by the proportional valve 11 .

[0020] Preferably, in the second solution, in the gradient mode, the way of achieving gradient elution can be controlled by the chromatographic pump 4 .

[0021] The above technical solution described in the present invention has the following advantages compared with the prior art:

[0022] ① The parallel chromatographic columns share a software control system to enable multiple chromatographic columns to run simultaneously, reducing the time for product analysis, separation and purification.

[0023] Reduce labor costs;

[0024] ② The parallel chromatographic columns share the mobile phase device, detector device, fraction collector and other devices to reduce equipment;

[0025] ③ The use of shared components reduces the factory area and lowers the load-bearing requirements, thereby reducing the load-bearing and area requirements of the factory and thus reducing construction costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of the automated chromatographic column system without a pre-column valve in the dual-column isocratic mode described in Example 1;

[0027] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 2-3, third mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank.

[0028] Figure 2 This is a schematic diagram of the structure of the automated chromatographic column system with a pre-column valve in the dual-column isocratic mode described in Example 2;

[0029] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 2-3, third mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank, 10, pre-column valve.

[0030] Figure 3 This is a schematic diagram of the structure of the automated chromatographic column system in the dual-column gradient mode described in Example 3;

[0031] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-A3, A3 mobile phase storage tank, 2-B1, B1 mobile phase storage tank, 2-B2, B2 mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve 2, 3-3, third multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank, 10 pre-column valve, 11-1, first proportional valve, 11-2, second proportional valve.

[0032] Figure 4 This is a schematic diagram of the structure of the automated chromatographic column system without a pre-column valve in the three-column isocratic mode described in Example 4;

[0033] Wherein: 1. sample tank, 2-1. first mobile phase storage tank, 2-2. second mobile phase storage tank, 2-3. third mobile phase storage tank, 3-1. first multi-way solvent selection valve, 3-2. second multi-way solvent selection valve, 3-3. third multi-way solvent selection valve, 4-1. first chromatographic pump, 4-2. second chromatographic pump, 4-3. third chromatographic pump, 5-1. first chromatographic column, 5-2. second chromatographic column, 5-3. third chromatographic column, 6. post-column valve, 7. detector device, 8. fraction collector, 9. waste liquid tank.

[0034] Figure 5 This is a schematic diagram of the structure of the automated chromatographic column system in the three-column gradient mode described in Example 5;

[0035] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-B, B mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 3-3, third multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank, 10-1, first pre-column valve, 10-2, second pre-column valve, 10-3, third pre-column valve, 11-1, first proportional valve, 11-2, second proportional valve.

[0036] Figure 6 This is a schematic diagram of the structure of the automated chromatographic column system without a column front valve in the four-column isocratic mode described in Example 6;

[0037] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 2-3, third mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 3-3, third multi-way solvent selection valve, 3-4, fourth multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 4-4, fourth chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 5-4, fourth chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank. Figure 7 This is a schematic diagram of the structure of an automated chromatographic column system without a pre-column valve and containing two sets of detectors in the four-column isocratic mode described in Example 7;

[0038] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 2-3, third mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 3-3, third multi-way solvent selection valve, 3-4, fourth multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 4-4, fourth chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 5-4, fourth chromatographic column, 6, post-column valve, 7-1, first detector, 7-2, second detector, 8-1, first fraction collector, 8-2, second fraction collector, 9, waste liquid tank.

[0039] Figure 8This is a schematic structural diagram of an automated chromatographic column system without a pre-column valve in a dual-column isocratic mode with only two mobile phase storage tanks as described in Example 8;

[0040] Wherein: 1. sample tank, 2-1. first mobile phase storage tank, 2-2. second mobile phase storage tank, 3-1. first multi-way solvent selection valve, 3-2. second multi-way solvent selection valve, 4-1. first chromatographic pump, 4-2. second chromatographic pump, 5-1. first chromatographic column, 5-2. second chromatographic column, 6. post-column valve, 7. detector device, 8. fraction collector, 9. waste liquid tank.

[0041] Fig. 9 This is a schematic diagram of the structure of the automated chromatographic column system in the dual-column gradient mode in which the dual pumps realize the gradient as described in Example 9;

[0042] Wherein: 1, sample tank, 2-1, first mobile phase storage tank, 2-2, second mobile phase storage tank, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump, 5-1, first chromatographic column, 5-2, second chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank, 10, pre-column valve. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention, such as: replacing the type and quantity of valves to achieve the same effect is within the scope.

[0044] Example 1

[0045] like Figure 1 The automated chromatographic column system without a pre-column valve in the dual-column isocratic mode shown includes a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a third mobile phase storage tank 2-3, a first multi-way solvent selection valve 3-1, a second multi-way solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9 and connecting pipes.

[0046] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2, and the third mobile phase storage tank 2-3, and is connected to the first multi-way solvent selection valve 3-1 and the second multi-way solvent selection valve 3-2 in parallel through connecting pipes. The multi-way solvent selection valves are correspondingly connected to the first chromatographic pump 4-1 and the second chromatographic pump 4-2 through connecting pipes; the chromatographic pumps are correspondingly connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipes, the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0047] The first mobile phase tank 2-1 is used for elution, the second mobile phase tank 2-2 is used for column washing, and the third mobile phase tank 2-3 is used for balancing. According to the characteristics of the process, there are mainly the following steps: column balancing (10 minutes) - injection (40 minutes) - elution (40 minutes) - column washing (10 minutes); according to the running time of each process, the adjacent running program combination is configured, the chromatographic column balancing, injection and elution, column washing time is equivalent, and the switching program is divided into two sections, namely: column balancing - injection and elution - column washing.

[0048] The workflow of this column system includes the following steps:

[0049] Step 1, balancing the first chromatographic column 5-1: switching the first multi-way solvent selection valve 3-1 to be connected to the third mobile phase tank 2-3, switching the post-column valve 6 to be connected to the waste liquid tank 9, starting the first chromatographic pump 4-1, and balancing the first chromatographic column 5-1 according to the time program requirements;

[0050] Step 2, injecting the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the sample tank, and operate other valves and pumps in the same way as in the previous steps, and inject the first chromatographic column 5-1 according to the time program requirements;

[0051] Step 3, elution of the first chromatographic column 5-1 - column washing: elution: switch the first multi-way solvent selection valve 3-1 to the first mobile phase tank 2-1, switch the post-column valve 6 to the detector device 7. Elute the first chromatographic column 5-1, detect through the detector device 7, and collect the sample by the fraction collector 8; column washing: after the elution time is completed, switch the first multi-way solvent selection valve 3-1 to the second mobile phase tank 2-2, and keep the other unchanged, wash the first chromatographic column 5-1 until the time program ends;

[0052] Step 4, balancing the second chromatographic column 5-2 - injection: when running the above steps, start this step at the same time, start the second chromatographic pump 4-2, the state of the post-column valve 6 is that the second chromatographic column 5-2 is connected to the waste liquid tank 9, and the first chromatographic column 5-1 is connected to the detector device 7. Balancing the second chromatographic column 5-2: the second multi-way solvent selection valve 3-2 is switched to the third mobile phase tank 2-3, and the second chromatographic column 5-2 is balanced by the second chromatographic pump 4-2; injection: after the balancing time of the second chromatographic column 5-2 is completed, switch the second multi-way solvent selection valve 3-2 to the sample tank 1, and inject the second chromatographic column 5-2 until the time program ends;

[0053] Step 5, cycle: that is, while the first chromatographic column 5-1 is equilibrated and injected, the second chromatographic column 5-2 is eluted and washed, the post-column valve 6 is switched to connect the first chromatographic column 5-1 to the waste liquid tank 9, and the equilibration-injection program of the first chromatographic column 5-1 is run through the time program; at this time, the second chromatographic column 5-2 is connected to the detector device 7 through the post-column valve 6, and the detector device 7 is connected to the fraction collector 8, and the elution-washing program of the second chromatographic column 5-2 is run through the time program; the two chromatographic columns are subjected to equilibration-injection-elution-washing according to the methods of steps 3, 4, and 5.

[0054] Example 2

[0055] like Figure 2 The automated chromatographic column system with a pre-column valve in the dual-column isocratic mode shown includes a sample tank, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a third mobile phase storage tank 2-3, a first multi-way solvent selection valve 3-1, a second multi-way solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, a pre-column valve 10 and connecting pipes.

[0056] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2, and the third mobile phase storage tank 2-3, and is connected to the first multi-way solvent selection valve 3-1 and the second multi-way solvent selection valve 3-2 in parallel through a connecting pipe. The multi-way solvent selection valve is connected to the pre-column valve 10 through a connecting pipe, and the pre-column valve 10 is connected to the first chromatographic pump 4-1 and the second chromatographic pump 4-2 in parallel; the chromatographic pump is connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 respectively through a connecting pipe, the chromatographic column is connected to the post-column valve 6 through a connecting pipe, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through a connecting pipe, and the detector device 7 is connected to the fraction collector 8 through a connecting pipe.

[0057] The first mobile phase tank 2-1 is used for elution; the second mobile phase tank 2-2 is used for column washing; the third mobile phase tank 2-3 is used for balance. According to the characteristics of the process, there are mainly the following steps: column balance (10 minutes) - injection (15 minutes) - elution (40 minutes) - column washing (10 minutes); according to the running time of each process, the adjacent running program combination is configured, the column balance, injection, and column washing time are basically five minutes shorter than the elution time, and continuous operation is required. It is planned to add a zero flow rate program, and after adding this program to the column washing program, the program will run in the following sections: column balance (10 minutes) - injection (15 minutes) - elution (40 minutes) - column washing (10 minutes) - zero flow rate (5 minutes). The switching program is divided into two sections, namely: column washing - zero flow rate - column balance - injection and elution.

[0058] The workflow of the column system includes the following steps:

[0059] Step 1, balancing the first chromatographic column 5-1: the first multi-way solvent selection valve 3-1 is connected to the third mobile phase tank 2-3, the pre-column valve 10 is switched to connect the first multi-way solvent valve 3-1 to the first chromatographic pump 4-1 through the pre-column valve 10, the post-column valve 6 is switched to the waste liquid tank 9, the mobile phase of the third mobile phase tank 2-3 is used, the first chromatographic pump 4-1 is started, and the first chromatographic column 5-1 is balanced according to the time program requirements;

[0060] Step 2, injecting the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the sample tank 1, and operate other valves and pumps in the same way as in the previous steps, and inject the first chromatographic column 5-1 according to the time program requirements;

[0061] Step 3, elution of the first chromatographic column 5-1: After the injection time is completed, switch the pre-column valve 10 to connect the first multi-way solvent valve 3-1 to the second chromatographic column 5-2, and connect the second multi-way solvent valve 3-2 to the second chromatographic column 5-1. Switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector 7 through the post-column valve 6, and finally collect the sample through the fraction collector 8, and the second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6. Switch the second multi-way solvent valve 3-2 to the first mobile phase tank 2-1, and start the first chromatographic pump 4-1 to elute the first chromatographic column 5-1 according to the time program.

[0062] Step 4, second chromatographic column 5-2 column washing - zero flow rate - chromatographic column balance - injection: when running the above steps, start this step at the same time. Since the chromatographic column is running for the first time and the chromatographic column has completed column washing, this section of the program can be set to zero flow rate (i.e., do not start the pump), or the chromatographic column can be cleaned again. This embodiment adopts the column washing method again. At this time, keep the column front valve 10 and the column rear valve 6 unchanged; column washing: start the second chromatographic pump 4-2, and run the second chromatographic pump 4-2 according to the column washing program; zero flow rate: switch the first multi-way solvent selection valve 3-1 to the second mobile phase tank 2-2, set the pump not to start, and wait for five minutes; chromatographic column balance: switch the first multi-way solvent selection valve 3-1 to the third mobile phase tank 2-3 pipeline, start the first chromatographic pump 4-2, and run according to the time program of this step; injection: switch the first multi-way solvent selection valve 3-1 to the sample tank pipeline, start the second chromatographic pump 4-2, and run according to the time program of this step;

[0063] Step 5, cycle: that is, the first chromatographic column 5-1 washes the column-zero flow rate-chromatographic column balance-injection, the second chromatographic column 5-2 is eluted, and the pre-column valve 10 and the post-column valve 6 are switched at the same time, so that the first multi-way solvent valve 3-1 is connected to the first chromatographic pump 4-1 through the pre-column valve 10, and continues to be connected to the waste liquid tank 9 after passing through the first chromatographic column 5-1. The second multi-way solvent selection valve 3-2 is connected to the first chromatographic pump 4-2 through the pre-column valve 10, and continues to be connected to the detector device 7 after passing through the second chromatographic column 5-2, and finally collects the sample through the fraction collector 8. The first chromatographic column 5-1 washes the column-zero flow rate-chromatographic column balance-injection program is run through the time program; at the same time, the second chromatographic column 5-2 elution program is run through the time program, and the cycle is repeated.

[0064] Example 3

[0065] like Figure 3 The automated chromatographic column system in the dual-column gradient mode shown includes a sample tank 1, an A1 mobile phase tank 2-A1, an A2 mobile phase tank 2-A2, an A3 mobile phase tank 2-A3, a B1 mobile phase tank 2-B1, a B2 mobile phase tank 2-B2, a first multi-way solvent selection valve 3-1, a second multi-way solvent selection valve 3-2, a third multi-way solvent selection valve 3-3, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, a pre-column valve 10, a first proportional valve 11-1, a second proportional valve 11-2 and connecting pipes.

[0066] The sample tank 1 is connected in parallel with the A1 mobile phase tank 2-A1, the A2 mobile phase tank 2-A2, the A3 mobile phase tank 2-A3, the B1 mobile phase tank 2-B1, and the B2 mobile phase tank 2-B2. The sample tank 1 is connected with the A1 mobile phase tank 2-A1, the A2 mobile phase tank 2-A2, the A3 mobile phase tank 2-A3, the B1 mobile phase tank 2-B1, and the B2 mobile phase tank 2-B2 through a connecting pipe to the first multi-way solvent selection valve 3-1. The A1 mobile phase tank 2-A1, the A2 mobile phase tank 2-A2, and the A3 mobile phase tank 2-A3 are connected to the second multi-way solvent selection valve 3-2. The B1 mobile phase tank 2-B1 and the B2 mobile phase tank 2-B2 are connected to the first multi-way solvent selection valve 3-1 through a connecting pipe. It is connected to the third multi-way solvent selection valve 3-3, the first multi-way solvent selection valve 3-1 is connected to the first chromatographic pump 4-1, the second multi-way solvent selection valve 3-2 and the third multi-way solvent selection valve 3-3 are respectively connected to the first proportional valve 11-1 and the second proportional valve 11-2 in parallel, and then connected to the second chromatographic pump 4-2, the chromatographic pump is then connected to the pre-column valve 10 through a connecting pipe, the pre-column valve 10 is correspondingly connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through a connecting pipe, the chromatographic column is connected to the post-column valve 6 through a connecting pipe, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through a connecting pipe, and the detector device 7 is connected to the fraction collector 8 through a connecting pipe.

[0067] According to the characteristics of the process, there are mainly the following steps: column equilibration (A1 mobile phase, 10 minutes) - injection (injection, 40 minutes) - elution 1 (A2 / B2 mobile phase gradient operation, 20 minutes) - elution 2 (A3 / B2 mobile phase gradient operation 40 minutes) - column washing (B1 mobile phase, 10 minutes). According to the running time of each process, the adjacent running program combination is configured, and the time for column washing, column equilibration, injection and elution 1 and elution 2 is equivalent. The switching program is divided into two sections, namely: column washing-equilibration-injection and elution 1-elution 2.

[0068] The workflow of this column system includes the following steps:

[0069] Step 1, washing the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the B1 mobile phase storage tank 2-B1, wash the first chromatographic column 5-1 through the first chromatographic pump 4-1 and the pre-column valve 10, and at the same time switch the post-column valve 6 to the waste liquid tank 9, and start the first chromatographic pump 4-1 to wash the first chromatographic column 5-1 according to the time program requirements;

[0070] Step 2, balancing the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the A1 mobile phase storage tank 2-A1, and operate other valves and pumps in the same way as in the previous step, and balance the first chromatographic column 5-1 according to the time program requirements;

[0071] Step 3, injecting the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the injection tank 1, and operate other valves and pumps in the same way as in the previous steps, and inject the first chromatographic column 5-1 according to the time program requirements;

[0072] Step 4, elution of the first chromatographic column 5-1 (elution 1-elution 2): Elution 1: switch the pre-column valve 10 to connect the second chromatographic pump 4-2 to the first chromatographic column 5-1, and at the same time, the first chromatographic pump 4-1 is connected to the second chromatographic column 5-2. Switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7; at the same time, the second chromatographic column 5-2 is connected to the waste liquid tank 9. Switch the second multi-way solvent selection valve 3-2 to connect to the mobile phase storage tank 2-A2, switch the third multi-way solvent selection valve 3-3 to connect to the mobile phase storage tank 2-B2, and run the elution 1 program according to the time program; Elution 2: keep the pre-column valve 10 and the post-column valve 6 unchanged, switch the second multi-way solvent selection valve 3-2 to connect to the mobile phase storage tank 2-A3, switch the third multi-way solvent selection valve 3-3 to connect to the mobile phase storage tank 2-B2, run the elution 2 program according to the time program, and finally collect the sample through the fraction collector 8;

[0073] Step 5, washing the second chromatographic column 5-2 - balancing - injecting: keep the pre-column valve 10 and the post-column valve 6 the same as the previous step. Run the current step while running the above steps. Washing the column: switch the first multi-way solvent selection valve 3-1 to the B1 mobile phase tank 2-B1, and wash the second chromatographic column 5-2 according to the time program through the first chromatographic pump 4-1; balancing: switch the first multi-way solvent selection valve 3-1 to the A1 mobile phase tank 2-A1, and balance the second chromatographic column 5-2 according to the time program through the first chromatographic pump 4-1; injection: switch the first multi-way solvent selection valve 3-1 to the injection tank 1, and inject the second chromatographic column 5-2 according to the time program through the first chromatographic pump 4-1;

[0074] Step 6, cycle: After completing the above two steps, switch the pre-column valve 10 and the post-column valve 6 at the same time, and perform column washing, balancing, and injection on the first chromatographic column 5-1 according to the time program, and perform elution 1-elution 2 on the second chromatographic column 5-2. Cycle the two chromatographic columns in this way.

[0075] Example 4

[0076] like Figure 4The automated chromatographic column system without a pre-column valve in the three-column isocratic mode shown includes a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a third mobile phase storage tank 2-3, a first multi-way solvent selection valve 3-1, a second multi-way solvent selection valve 3-2, a third multi-way solvent selection valve 3-3, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a third chromatographic pump 4-3, a first chromatographic column 5-1, a second chromatographic column 5-2, a third chromatographic column 5-3, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9 and connecting pipes.

[0077] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2, and the third mobile phase storage tank 2-3, and is connected to the first multi-way solvent selection valve 3-1, the second multi-way solvent selection valve 3-2, and the third multi-way solvent selection valve 3-3 in parallel through connecting pipes. The multi-way solvent selection valves are correspondingly connected to the first chromatographic pump 4-1, the second chromatographic pump 4-2, and the third chromatographic pump 4-3 through connecting pipes; the chromatographic pumps are correspondingly connected to the first chromatographic column 5-1, the second chromatographic column 5-2, and the third chromatographic column 5-3 through connecting pipes, the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0078] According to the characteristics of the process, there are mainly the following steps: column balancing (15 minutes) - injection (40 minutes) - elution (40 minutes) - column washing (15 minutes); according to the running time of each process, configure adjacent running program combinations, column washing, column balancing, injection and elution are three equivalent time periods, adjust the column washing and column balancing time to 20 minutes, and divide the switching program into three sections, namely: column washing (20 minutes) - column balancing (20 minutes), injection (40 minutes), elution (40 minutes); 1. Sample tank, tank 2-1 is used for elution, tank 2-2 is used for column washing, and tank 2-3 is used for balancing.

[0079] The workflow of this column system includes the following steps:

[0080] Step 1, first chromatographic column 5-1 column washing-equilibration.

[0081] Washing and balancing the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the second mobile phase storage tank 2-2, switch the post-column valve 6 to the waste liquid tank 9, use the second mobile phase storage tank 2-2, start the first chromatographic pump 4-1, and wash the first chromatographic column 5-1 according to the time program requirements; Balancing the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the third mobile phase storage tank 2-3, keep switching the post-column valve 6 to the waste liquid tank 9, use the balanced mobile phase, start the first chromatographic pump 4-1, and balance the first chromatographic column 5-1 according to the time program requirements;

[0082] Step 2, injecting samples into the first chromatographic column 5-1 and washing and balancing the second chromatographic column 5-2.

[0083] Injection of the first chromatographic column 5-1: switch the first multi-way solvent selection valve 3-1 to the sample tank 1, and operate other valves and pumps in the same way as in the previous steps, and inject the first chromatographic column 5-1 according to the time program requirements; Column washing and column balancing of the second chromatographic column 5-2: when the first chromatographic column 5-1 is injected, start the second chromatographic pump 4-2, switch the second multi-way solvent selection valve 3-2, and perform column washing and column balancing of the second chromatographic column 5-2 according to the time program;

[0084] Step 3, eluting the first chromatographic column 5-1, injecting the sample into the second chromatographic column 5-2, and washing the third chromatographic column 5-3 - balancing the chromatographic column.

[0085] Elution of the first chromatographic column 5-1: switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7, switch the first multi-way solvent selection valve 3-1 to the first mobile phase storage tank 2-1, operate the first chromatographic pump 4-1, elute the first chromatographic column 5-1 according to the time program requirements, and finally collect the sample through the fraction collector 8; injection of the second chromatographic column 5-2: while executing the above steps, start the second chromatographic pump 4-2, switch the second multi-way solvent selection valve 3-2 to the injection tank 1, and inject the second chromatographic column 5-2 according to the time program requirements; washing the third chromatographic column 5-3 - balancing the chromatographic column: when eluting the first chromatographic column 5-1 and injecting the second chromatographic column 5-2, start the third chromatographic pump 4-3, switch the third multi-way solvent selection valve 3-3 and perform washing and balancing of the third chromatographic column 5-3 according to the time program;

[0086] Step 4, washing the first chromatographic column 5-1 - balancing the chromatographic column, eluting the second chromatographic column 5-2, and injecting the sample into the second chromatographic column 5-3.

[0087] Washing the first chromatographic column 5-1 - balancing the chromatographic column: when the elution time program of the first chromatographic column 5-1 is completed, switch the post-column valve 6 to connect the second chromatographic column 5-2 to the detector device 7, start the first chromatographic pump 4-1, and switch the first multi-way solvent selection valve 3-1 according to the time program to perform washing and balancing on the first chromatographic column 5-1; Elution of the second chromatographic column 5-2: while running the above steps, start the second chromatographic pump 4-2, switch the second multi-way solvent selection valve 3-2 to the mobile phase storage tank 2-1, elute the second chromatographic column 5-2 according to the time program requirements, and finally collect samples through the fraction collector 8; Injection of the third chromatographic column 5-3: while running the above steps, start the third chromatographic pump 4-3, switch the third multi-way solvent selection valve 3-3 to the sample tank 1, and inject the third chromatographic column 5-3 according to the time program requirements;

[0088] Step 5, injecting samples into the first chromatographic column 5-1, washing the second chromatographic column 5-2 - balancing the chromatographic column, and eluting the third chromatographic column 5-3.

[0089] Injection of the first chromatographic column 5-1: when the above step 4 is completed, the post-column valve 6 is switched to connect the third chromatographic column 5-3 to the detector device 7, the first chromatographic pump 4-1 is started, and the first multi-way solvent selection valve 3-1 is switched to inject the first chromatographic column 5-1 according to the time program; washing the second chromatographic column 5-2-chromatographic column balance: while running the above steps, the second chromatographic pump 4-2 is started, and the second multi-way solvent selection valve 3-2 is switched to the mobile phase storage tank 2-2 and the mobile phase storage tank 2-3 in sequence according to the time program requirements, and the second chromatographic column 5-2 is washed and balanced; elution of the third chromatographic column 5-3: while running the above steps, the third chromatographic pump 4-3 is started, and the third multi-way solvent selection valve 3-3 is switched to the mobile phase storage tank 2-1, and the third chromatographic column 5-3 is eluted according to the time program requirements, and finally the sample is collected through the fraction collector 8;

[0090] Step 6, cycle: According to the above three steps, cycle the three chromatographic columns through elution - column equilibration - injection - column washing.

[0091] Example 5

[0092] like Figure 5 The automated column system shown in three-column gradient mode includes

[0093] Wherein: 1, sample tank, 2-A1, A1 mobile phase storage tank, 2-A2, A2 mobile phase storage tank, 2-B, B mobile phase storage tank, 3-1, first multi-way solvent selection valve, 3-2, second multi-way solvent selection valve, 3-3, third multi-way solvent selection valve, 4-1, first chromatographic pump, 4-2, second chromatographic pump, 4-3, third chromatographic pump (high-precision pump), 5-1, first chromatographic column, 5-2, second chromatographic column, 5-3, third chromatographic column, 6, post-column valve, 7, detector device, 8, fraction collector, 9, waste liquid tank, 10-1, first pre-column valve, 10-2, second pre-column valve, 10-3, third pre-column valve, 11-1, first proportional valve, 11-2, second proportional valve and connecting pipes.

[0094] The sample tank 1 is connected in parallel with the A1 mobile phase storage tank 2-A1, the A2 mobile phase storage tank 2-A2, and the B1 mobile phase storage tank 2-B1, and then connected with the first multi-way solvent selection valve 3-1 and the second multi-way solvent selection valve 3-2 connected in parallel, and the first multi-way solvent selection valve 3-1 and the second multi-way solvent selection valve 3-2 are connected with the first chromatographic pump 4-1 and the second chromatographic pump 4-2 connected in parallel respectively; the A1 mobile phase storage tank 2-A1 and the A2 mobile phase storage tank 2-A2 are connected with the third multi-way solvent selection valve 3-3, and the third multi-way solvent selection valve 3-3 is connected with the first proportional valve 11-1, and the B The mobile phase storage tank 2-B1 is connected to the second proportional valve 11-2, and the proportional valve is connected to the third chromatographic pump 4-3; the chromatographic pump is then connected to the first pre-column valve 10-1, the second pre-column valve 10-2, and the third pre-column valve 10-3 connected in parallel through connecting pipes, the pre-column valve 10 is connected to the first chromatographic column 5-1, the second chromatographic column 5-2, and the third chromatographic column 5-3 through connecting pipes, the chromatographic column is connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0095] According to the characteristics of the process, there are mainly the following steps: chromatographic column equilibration (first mobile phase tank 2-A1, 15 minutes) - chromatographic injection (sample tank 1, 40 minutes) - elution (second mobile phase tank 2-A2 / third mobile phase tank 2-B gradient operation, 40 minutes) - column washing (third mobile phase tank 2-B, 15 minutes); according to the running time of each process, configure the adjacent running program combination, and the switching program is divided into three sections, namely: column washing - equilibration, injection, elution. In order to switch the three columns, adjust the running time of each section to be the same, as follows: column washing (20 minutes) - equilibration (20 minutes); injection (40 minutes); elution (40 minutes). Elution program: When the chromatographic column runs the elution program, use the third chromatographic pump 4-3 (high-precision pump) and connect the detector device through the post-column valve 6.

[0096] Step 1, first chromatographic column 5-1 washing column-equilibrium:

[0097] Switch the first multi-channel solvent selection valve 3-1 to the third mobile phase storage tank 2-B, start the first chromatographic pump 4-1, wash the first chromatographic column 5-1 according to the time program through the first column front valve 10-1, keep the first column front valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase storage tank 2-A1, and balance the chromatographic column according to the balance time program;

[0098] Step 2: Wash and balance the second chromatographic column 5-2 and inject the sample into the first chromatographic column 5-1

[0099] Switch the first multi-channel solvent selection valve 3-1 to the third mobile phase storage tank 2-B, start the first chromatographic pump 4-1, and wash the second chromatographic column 5-2 through the first column front valve 10-1 according to the time program. Keep the first column front valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase storage tank 2-A1, and balance the chromatographic column according to the balance time program;

[0100] While the above operation is being performed, the second multi-way solvent selection valve 3-2 is switched to the sample tank 1, the second chromatographic pump 4-2 is started, and the first chromatographic column 5-1 is injected according to the time program through the second pre-column valve 10-2;

[0101] Step 3: washing and balancing the third chromatographic column 5-3, injecting the sample into the second chromatographic column 5-2, and eluting the first chromatographic column 5-1

[0102] Switch the first multi-channel solvent selection valve 3-1 to the third mobile phase storage tank 2-B, start the first chromatographic pump 4-1, and wash the third chromatographic column 5-3 through the first column front valve 10-1 according to the time program. Keep the first column front valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase storage tank 2-A1, and balance the chromatographic column according to the balance time program;

[0103] While the above operation is being performed, the second multi-way solvent selection valve 3-2 is switched to the sample tank 1, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is injected according to the time program through the second pre-column valve 10-2;

[0104] While the above operations are being performed, the third multi-channel solvent selection valve 3-3 is switched to the second mobile phase storage tank 2-A2, the third chromatographic pump 4-3 is started, and the first chromatographic column 5-1 is eluted according to the time program through the third pre-column valve 10-3. At this time, the post-column valve 6 is switched to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0105] Step 4, injection into the third chromatographic column 5-3, elution into the second chromatographic column 5-2, washing and balancing the first chromatographic column 5-1; switching the second multi-way solvent selection valve 3-2 to the sample tank 1, starting the second chromatographic pump 4-2, and injecting into the third chromatographic column 5-3 through the second pre-column valve 10-2 according to the time program;

[0106] While the above operations are being performed, the third multi-channel solvent selection valve 3-3 is switched to the second mobile phase storage tank 2-A2, the third chromatographic pump 4-3 is started, and the second chromatographic column 5-2 is eluted according to the time program through the third pre-column valve 10-3. At this time, the post-column valve 6 is switched to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0107] While performing the above operations, switch the first multi-channel solvent selection valve 3-1 to the third mobile phase storage tank 2-B, start the first chromatographic pump 4-1, and wash the first chromatographic column 5-1 through the first column front valve 10-1 according to the time program. Keep the first column front valve 10-1 unchanged, switch the first multi-channel solvent selection valve 3-1 to the first mobile phase storage tank 2-A1, and balance the chromatographic column according to the balance time program;

[0108] Step 5, elution of the third chromatographic column 5-3, washing and balancing of the second chromatographic column 5-2, injection of the first chromatographic column 5-1, switching the third multi-channel solvent selection valve 3-3 to the second mobile phase storage tank 2-A2, starting the third chromatographic pump 4-3, eluting the third chromatographic column 5-3 according to the time program through the third pre-column valve 10-3, at this time, the post-column valve 6 is switched to the detector device 7, and finally the sample is collected through the fraction collector 8.

[0109] While the above operations are being performed, the first multi-channel solvent selection valve 3-1 is switched to the third mobile phase storage tank 2-B, the first chromatographic pump 4-1 is started, and the second chromatographic column 5-2 is washed according to the time program through the first column front valve 10-1. The first column front valve 10-1 is kept unchanged, the first multi-channel solvent selection valve 3-1 is switched to the first mobile phase storage tank 2-A1, and the chromatographic column is balanced according to the balance time program; while the above operations are being performed, the second multi-channel solvent selection valve 3-2 is switched to the sample tank 1, the second chromatographic pump 4-2 is started, and the first chromatographic column 5-1 is injected according to the time program through the second column front valve 10-2;

[0110] Cycle: Wash the three chromatographic columns in a cycle according to the above method - balance, load sample, and elute.

[0111] Example 6

[0112] like Figure 6 The automated column system shown in the four-column isocratic mode without a pre-column valve includes

[0113] Sample tank 1, first mobile phase storage tank 2-1, second mobile phase storage tank 2-2, third mobile phase storage tank 2-3, first multi-way solvent selection valve 3-1, second multi-way solvent selection valve 3-2, third multi-way solvent selection valve 3-3, fourth multi-way solvent selection valve 3-4, first chromatographic pump 4-1, second chromatographic pump 4-2, third chromatographic pump 4-3, fourth chromatographic pump 4-4, first chromatographic column 5-1, second chromatographic column 5-2, third chromatographic column 5-3, fourth chromatographic column 5-4, post-column valve 6, detector device 7, fraction collector 8, waste liquid tank 9 and connecting pipes.

[0114] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2, and the third mobile phase storage tank 2-3, and is connected to the first multi-way solvent selection valve 3-1, the second multi-way solvent selection valve 3-2, the third multi-way solvent selection valve 3-3, and the fourth multi-way solvent selection valve 3-4 in parallel through connecting pipes. The multi-way solvent selection valves are correspondingly connected to the first chromatographic pump 4-1, the second chromatographic pump 4-2, the third chromatographic pump 4-3, and the fourth chromatographic pump 4-4 through connecting pipes; the chromatographic pumps are correspondingly connected to the first chromatographic column 5-1, the second chromatographic column 5-2, the third chromatographic column 5-3, and the fourth chromatographic column 5-4 through connecting pipes, the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0115] According to the characteristics of the process, there are mainly the following steps: column balancing (25 minutes) - injection (30 minutes) - elution (30 minutes) - column washing (25 minutes). According to the running time of each process, the running time is adjusted and the switching program is divided into four sections, namely: column balancing (30 minutes), injection (30 minutes), elution (30 minutes), column washing (30 minutes);

[0116] When the chromatographic column elution program is running, the post-column valve 6 is switched to connect the chromatographic column to the detector device 7 , and the sample is collected through the fraction collector 8 .

[0117] Step 1: Equilibration of the first chromatographic column 5-1

[0118] Switch the first solvent selection valve 3-1 to connect to the third mobile phase storage tank 2-3, switch the post-column valve 6 to connect to the waste liquid tank 9, use the balanced mobile phase, start the first chromatographic pump 4-1, and balance the first chromatographic column 5-1 according to the time program requirements;

[0119] Step 2: Injection into the first chromatographic column 5-1 and balance the second chromatographic column 5-2

[0120] Switch the first solvent selection valve 3-1 to connect to the sample injection tank 1, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the waste liquid tank 9, start the first chromatographic pump 4-1, and inject the sample into the first chromatographic column 5-1 according to the time program requirements;

[0121] While the above operation is being performed, the second solvent selection valve 3-2 is switched to connect to the third mobile phase storage tank 2-3, a balanced mobile phase is used, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is balanced according to the time program requirements;

[0122] Step 3: elution from the first chromatographic column 5-1, injection from the second chromatographic column 5-2, and balance from the third chromatographic column 5-3

[0123] Switch the first solvent selection valve 3-1 to connect to the first mobile phase storage tank 2-1, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7, finally collect the sample through the fraction collector 8, use the elution mobile phase, start the first chromatographic pump 4-1, and elute the first chromatographic column 5-1 according to the time program requirements;

[0124] While the above operations are being performed, the second solvent selection valve 3-2 is switched to connect to the injection tank 1, the post-column valve 6 is switched to connect the second chromatographic column 5-2 to the waste liquid tank 9, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is injected according to the time program requirements;

[0125] While the above operation is being performed, the third solvent selection valve 3-3 is switched to connect to the third mobile phase storage tank 2-3, a balanced mobile phase is used, the third chromatographic pump 4-3 is started, and the third chromatographic column 5-3 is balanced according to the time program requirements;

[0126] Step 4, the first chromatographic column 5-1 is washed, the second chromatographic column 5-2 is eluted, the third chromatographic column 5-3 is injected, and the fourth chromatographic column 5-4 is balanced. The first multi-way solvent selection valve 3-1 is switched to connect with the second mobile phase storage tank 2-2, and the post-column valve 6 is switched to connect the first chromatographic column 5-1 with the waste liquid tank 9. The second mobile phase storage tank 2-2 is used, and the first chromatographic pump 4-1 is started to wash the first chromatographic column 5-1 according to the time program requirements;

[0127] While the above operations are being performed, the second multi-way solvent selection valve 3-2 is switched to connect to the first mobile phase tank 2-1, the post-column valve is switched so that the second chromatographic column 5-2 is connected to the detector device 7, and finally the fraction collector 8 is used to collect the sample, the elution mobile phase is used, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is eluted according to the time program requirements;

[0128] While the above operations are being performed, the third multi-way solvent selection valve 3-3 is switched to the injection tank 1, the post-column valve 6 is switched so that the third chromatographic column 5-3 is connected to the waste liquid tank 9, the third chromatographic pump 4-3 is started, and the third chromatographic column 5-3 is injected according to the time program requirements;

[0129] While the above operation is being performed, the fourth multi-way solvent selection valve 3-4 is switched to connect to the mobile phase storage tank 2-3, a balanced mobile phase is used, the fourth chromatographic pump 4-4 is started, and the fourth chromatographic column 5-4 is balanced according to the time program requirements;

[0130] Step 5, balance the first chromatographic column 5-1, wash the second chromatographic column 5-2, elute the third chromatographic column 5-3, and inject the fourth chromatographic column 5-4. Switch the first solvent selection valve 3-1 to connect with the mobile phase storage tank 2-3, switch the post-column valve 6 to connect the first chromatographic column 5-1 with the waste liquid tank 9, use the balanced mobile phase, start the first chromatographic pump 4-1, and balance the first chromatographic column 5-1 according to the time program requirements;

[0131] While the above operations are being performed, the second multi-way solvent selection valve 3-2 is switched to connect to the second mobile phase storage tank 2-2, the post-column valve 6 is switched to connect the second chromatographic column 5-2 to the waste liquid tank 9, the column washing mobile phase is used, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is washed according to the time program requirements;

[0132] While the above operations are being performed, the third multi-way solvent selection valve 3-3 is switched to connect to the first mobile phase tank 2-1, the post-column valve 6 is switched to connect the third chromatographic column 5-3 to the detector device 7, the fraction collector 8 is used to collect the sample, the elution mobile phase is used, the third chromatographic pump 4-3 is started, and the third chromatographic column 5-3 is eluted according to the time program requirements;

[0133] While the above operations are being performed, the fourth solvent selection valve 3-4 is switched to the sample tank 1 pipeline, the fourth chromatographic pump 4-4 is started, and the fourth chromatographic column 5-4 is injected according to the time program requirements;

[0134] Step 6, injecting into the first chromatographic column 5-1, balancing the second chromatographic column 5-2, washing the third chromatographic column 5-3, and eluting the fourth chromatographic column 5-4. Switch the first solvent selection valve 3-1 to connect with the sample tank 1, switch the post-column valve 6 to connect the first chromatographic column 5-1 with the waste liquid tank 9, start the first chromatographic pump 4-1, and inject into the first chromatographic column 5-1 according to the time program requirements;

[0135] While the above operations are being performed, the second solvent selection valve 3-2 is switched to connect to the mobile phase storage tank 2-3, the post-column valve 6 is switched to connect the second chromatographic column 5-2 to the waste liquid tank 9, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is balanced according to the time program requirements;

[0136] While the above operations are being performed, the third multi-way solvent selection valve 3-3 is switched to connect to the second mobile phase storage tank 2-2, the post-column valve 6 is switched to connect the third chromatographic column 5-3 to the waste liquid tank 9, the third chromatographic pump 4-3 is started, and the third chromatographic column 5-3 is washed according to the time program requirements;

[0137] While the above operations are being performed, the fourth solvent selection valve 3-4 is switched to connect to the first mobile phase tank 2-1, the post-column valve 6 is switched to connect the fourth chromatographic column 5-4 to the detector device 7, the fraction collector 8 is used to collect the sample, the fourth chromatographic pump 4-4 is started, and the fourth chromatographic column 5-4 is eluted according to the time program requirements;

[0138] Step 7, elution of the first chromatographic column 5-1, injection of the second chromatographic column 5-2, equilibration of the third chromatographic column 5-3, and washing of the fourth chromatographic column 5-4. Switch the first solvent selection valve 3-1 to connect with the first mobile phase tank 2-1, switch the post-column valve 6 to connect the first chromatographic pump 4-1 with the detector device 7, use the fraction collector 8 to collect samples, use the elution mobile phase, and start the first chromatographic pump 4-1. Elute the first chromatographic column 5-1 according to the time program requirements;

[0139] While the above operations are being performed, the second solvent selection valve 3-2 is switched to connect to the sample tank 1, the post-column valve 6 is switched to connect the second chromatographic column 5-2 to the waste liquid tank 9, the second chromatographic pump 4-2 is started, and the second chromatographic column 5-2 is injected according to the time program requirements;

[0140] While the above operations are being performed, the third solvent selection valve 3-3 is switched to connect to the mobile phase storage tank 2-3, the post-column valve 6 is switched to connect the third chromatographic column 5-3 to the waste liquid tank 9, the third chromatographic pump 4-3 is started, and the third chromatographic column 5-3 is balanced according to the time program requirements;

[0141] While the above operations are being performed, the fourth solvent selection valve 3-4 is switched to connect to the second mobile phase storage tank 2-2, the post-column valve 6 is switched to connect the fourth chromatographic column 5-4 to the waste liquid tank 9, the fourth chromatographic pump 4-4 is started, and the fourth chromatographic column 5-4 is washed according to the time program requirements;

[0142] Step 8, cycle: According to the above four steps, cycle the four chromatographic columns through column equilibration - injection - elution - column washing, and collect samples.

[0143] Example 7

[0144] like Figure 7 The automated column system shown in the four-column isocratic mode has no pre-column valve and contains two sets of detectors.

[0145] Sample tank 1, first mobile phase storage tank 2-1, second mobile phase storage tank 2-2, third mobile phase storage tank 2-3, first multi-way solvent selection valve 3-1, second multi-way solvent selection valve 3-2, third multi-way solvent selection valve 3-3, fourth multi-way solvent selection valve 3-4, first chromatographic pump 4-1, second chromatographic pump 4-2, third chromatographic pump 4-3, fourth chromatographic pump 4-4, first chromatographic column 5-1, second chromatographic column 5-2, third chromatographic column 5-3, fourth chromatographic column 5-4, post-column valve 6, first detector 7-1, second detector 7-8, first fraction collector 8-1, second fraction collector 8-2, waste liquid tank 9 and connecting pipes.

[0146] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1, the second mobile phase storage tank 2-2, and the third mobile phase storage tank 2-3, and is connected to the first multi-way solvent selection valve 3-1, the second multi-way solvent selection valve 3-2, the third multi-way solvent selection valve 3-3, and the fourth multi-way solvent selection valve 3-4 in parallel through connecting pipes. The multi-way solvent selection valves are correspondingly connected to the first chromatographic pump 4-1, the second chromatographic pump 4-2, the third chromatographic pump 4-3, and the fourth chromatographic pump 4-4 through connecting pipes; the chromatographic pumps are correspondingly connected to the first chromatographic column 5-1, the second chromatographic column 5-2, the third chromatographic column 5-3, and the fourth chromatographic column 5-4 through connecting pipes, the chromatographic column is connected to the post-column valve 6 through the connecting pipe, the post-column valve 6 is connected to the first detector 7-1, the second detector 7-2, and the waste liquid tank 9 in parallel through the connecting pipe, and the first detector 7-1 and the second detector 7-2 are respectively connected to the first fraction collector 8-1 and the second fraction collector 8-2 through connecting pipes.

[0147] According to the characteristics of the process, there are mainly the following steps: column balancing (third mobile phase tank 2-3, 10 minutes) - injection (sample tank 1, 40 minutes) - elution (first mobile phase tank 2-1, 60 minutes) - column washing (second mobile phase tank 2-2, 10 minutes). The elution time is relatively long, and two sets of detector devices and fraction collectors are configured. According to the running time of each process, the switching program is divided into two sections, namely: column washing (10 minutes) - column balancing (10 minutes) - injection (40 minutes) and elution (60 minutes);

[0148] Elution program: When the chromatographic column runs the elution program, the post-column valve 6 is connected to the detector device (7-1, 7-2) and the sample is collected through the fraction collector.

[0149] The workflow of this column system includes the following steps:

[0150] Step 1, washing the first chromatographic column 5-1 - balancing the chromatographic column - injection, washing the third chromatographic column 5-3 - balancing the chromatographic column - injection. Start the first chromatographic pump 4-1 to operate the first chromatographic column 5-1, switch the first multi-channel solvent selection valve 3-1 to the second mobile phase storage tank 2-2 for washing the column, the third mobile phase storage tank 2-3 for balancing the chromatographic column, and the sample tank 1 for injection according to the time program, and the first chromatographic column 5-1 passes through the post-column valve 6 to the waste liquid tank 9;

[0151] At the same time, the third chromatographic pump 4-3 is started to operate the first chromatographic column 5-3, and the third multi-channel solvent selection valve 3-3 is switched to the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column balance, and the sample tank 1 for sample injection according to the time program, and the third chromatographic column 5-3 is connected to the waste liquid tank 9 through the post-column valve 6;

[0152] Step 2, eluting the first chromatographic column 5-1, eluting the third chromatographic column 5-3, washing the second chromatographic column 5-2 - balancing the chromatographic column - injecting, washing the fourth chromatographic column 5-2 - balancing the chromatographic column - injecting,

[0153] Start the first chromatographic pump 4-1 to elute the first chromatographic column 5-1, switch the first multi-channel solvent selection valve 3-1 to connect to the first mobile phase storage tank 2-1 to elute according to the time program, connect the first chromatographic column 5-1 to the first detector device 7-1 through the post-column valve 6, and collect samples through the first fraction collector 8-1;

[0154] At the same time, the third chromatographic pump 4-3 is started to elute the third chromatographic column 5-3, the third multi-channel solvent selection valve 3-3 is switched to connect to the first mobile phase storage tank 2-1 to elute according to the time program, the third chromatographic column 5-3 is connected to the first detector device 7-2 through the post-column valve 6, and the sample is collected through the first fraction collector 8-2;

[0155] At the same time, the second chromatographic pump 4-2 is started to operate the second chromatographic column 5-2, and the second multi-channel solvent selection valve 3-2 is switched to connect the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6;

[0156] At the same time, the fourth chromatographic pump 4-4 is started to operate the fourth chromatographic column 5-4, and the fourth multi-channel solvent selection valve 3-4 is switched to connect the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the fourth chromatographic column 5-4 is connected to the waste liquid tank 9 through the post-column valve 6;

[0157] Step 3, first chromatographic column 5-1 washing column - chromatographic column balance - injection, third chromatographic column 5-3 washing column - chromatographic column balance - injection, second chromatographic column 5-2 elution, fourth chromatographic column 5-2 elution, start the first chromatographic pump 4-1, switch the first multi-channel solvent selection valve 3-1 to connect the second mobile phase storage tank 2-2 washing column, the third mobile phase storage tank 2-3 chromatographic column balance, sample tank 1 injection according to the time program, and the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6;

[0158] At the same time, the third chromatographic pump 4-3 is started, and the third multi-channel solvent selection valve 3-3 is switched to the second mobile phase storage tank 2-2 for column washing, the third mobile phase storage tank 2-3 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the third chromatographic column 5-3 is connected to the waste liquid tank 9 through the post-column valve 6;

[0159] At the same time, the second chromatographic pump 4-2 is started, the second multi-channel solvent selection valve 3-2 is switched to connect to the first mobile phase storage tank 2-1 for elution according to the time program, the second chromatographic column 5-2 is connected to the first detector device 7-1 through the post-column valve 6, and the sample is collected through the first fraction collector 8-1;

[0160] At the same time, the fourth chromatographic pump 4-4 is started, the fourth multi-channel solvent selection valve 3-4 is switched to connect to the first mobile phase storage tank 2-1 for elution according to the time program, the fourth chromatographic column 5-4 is connected to the second detector device 7-2 through the post-column valve 6, and the sample is collected through the second fraction collector 8-2;

[0161] Step 4, cycle: According to the above two steps, cycle the four chromatographic columns through column equilibration - injection - elution - column washing, and collect samples.

[0162] Example 8

[0163] like Figure 8 The automated chromatographic column system without a pre-column valve in a dual-column isocratic mode with only two mobile phase storage tanks comprises a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a first multi-way solvent selection valve 3-1, a second multi-way solvent selection valve 3-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9 and connecting pipes.

[0164] The sample tank 1 is connected in parallel with the first mobile phase storage tank 2-1 and the second mobile phase storage tank 2-2, and is connected to the first multi-way solvent selection valve 3-1 and the second multi-way solvent selection valve 3-2 in parallel through connecting pipes. The multi-way solvent selection valves are correspondingly connected to the first chromatographic pump 4-1 and the second chromatographic pump 4-2 through connecting pipes; the chromatographic pumps are correspondingly connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipes, the chromatographic columns are connected to the post-column valve 6 through connecting pipes, the post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes, and the detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0165] According to the characteristics of the process, there are mainly the following steps: column equilibration (first mobile phase tank 2-1, 10 minutes) - injection (sample tank 1, 20 minutes) - elution (second mobile phase tank 2-2, 40 minutes) - column washing (second mobile phase tank 2-2, 10 minutes). According to the running time of each process, the switching program is divided into two sections, namely: column washing (10 minutes) - column equilibration (10 minutes) - injection (20 minutes) and elution (40 minutes);

[0166] The workflow of this column system includes the following steps:

[0167] Step 1: First chromatographic column 5-1: Column washing - equilibration - injection

[0168] Start the first chromatographic pump 4-1 to operate the first chromatographic column 5-1, switch the first multi-channel solvent selection valve 3-1 to the second mobile phase storage tank 2-2 for column washing, the first mobile phase storage tank 2-1 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6;

[0169] Step 2, elution of the first chromatographic column 5-1, washing of the second chromatographic column 5-2 - equilibration - injection:

[0170] Start the first chromatographic pump 4-1, switch the first multi-channel solvent selection valve 3-1 to connect to the second mobile phase storage tank 2-2 to elute the chromatographic column according to the time program, connect the first chromatographic column 5-1 to the detector device 7 through the post-column valve 6, and collect samples through the fraction collector 8;

[0171] At the same time, the second chromatographic pump 4-2 is started, and the second multi-channel solvent selection valve 3-2 is switched to connect the second mobile phase storage tank 2-2 for column washing, the first mobile phase storage tank 2-1 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the second chromatographic column 5-2 is connected to the waste liquid tank 9 through the post-column valve 6;

[0172] Step 3: washing the first chromatographic column 5-1, balancing, injecting, and eluting the second chromatographic column 5-2

[0173] Start the first chromatographic pump 4-1, switch the first multi-channel solvent selection valve 3-1 to connect the second mobile phase storage tank 2-2 for column washing, the first mobile phase storage tank 2-1 for chromatographic column balancing, and the sample tank 1 for sample injection according to the time program, and the first chromatographic column 5-1 is connected to the waste liquid tank 9 through the post-column valve 6;

[0174] At the same time, the second chromatographic pump 4-2 is started, the second multi-channel solvent selection valve 3-2 is switched to connect to the second mobile phase storage tank 2-2 to elute the chromatographic column according to the time program, the second chromatographic column 5-2 is connected to the detector device 7 through the post-column valve 6, and the sample is collected through the fraction collector 8;

[0175] Step 4, cycle: According to the above two steps, cycle the two chromatographic columns through column equilibration - injection - elution - column washing, and collect samples.

[0176] Example 9

[0177] like Fig. 9The dual-pump automatic chromatographic column system in the dual-column gradient mode for realizing gradient comprises a sample tank 1, a first mobile phase storage tank 2-1, a second mobile phase storage tank 2-2, a first chromatographic pump 4-1, a second chromatographic pump 4-2, a third chromatographic pump 4-3, a first chromatographic column 5-1, a second chromatographic column 5-2, a post-column valve 6, a detector device 7, a fraction collector 8, a waste liquid tank 9, a pre-column valve 10 and connecting pipes.

[0178] The sample tank 1, the first mobile phase storage tank 2-1, and the second mobile phase storage tank 2-2 are connected in parallel. The sample tank 1 is connected to the first chromatographic pump 4-1 and then to the pre-column valve 10. The first mobile phase storage tank 2-1 and the second mobile phase storage tank 2-2 are respectively connected to the second chromatographic pump 4-2 and the third chromatographic pump 4-3. The second chromatographic pump 4-2 and the third chromatographic pump 4-3 are connected to the pre-column valve 10 through connecting pipes. The pre-column valve 10 is correspondingly connected to the first chromatographic column 5-1 and the second chromatographic column 5-2 through connecting pipes. The chromatographic column is connected to the post-column valve 6 through connecting pipes. The post-column valve 6 is connected to the parallel detector device 7 and the waste liquid tank 9 through connecting pipes. The detector device 7 is connected to the fraction collector 8 through connecting pipes.

[0179] According to the characteristics of the process, there are mainly the following steps: column balancing (10% first mobile phase tank 2-1 / 90% second mobile phase tank 2-2, 10 minutes) - injection (sample tank 1, 50 minutes) - elution (60% first mobile phase tank 2-1 / 40% second mobile phase tank 2-2, 30 minutes) - column washing (80% first mobile phase tank 2-1 / 20% second mobile phase tank 2-2, 10 minutes). According to the running time of each process, the switching program is divided into two sections, namely: injection (50 minutes) and elution (30 minutes) - column washing (10 minutes) - column balancing (10 minutes). Different proportions of mobile phase are achieved by running two pumps at different flow rates.

[0180] The workflow of this column system includes the following steps:

[0181] Step 1: Balance the first chromatographic column 5-1 and the second chromatographic column 5-2

[0182] Start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve 10 to connect to the first chromatographic column 5-1, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the waste liquid tank 9, operate the chromatographic pumps according to the time program and the mobile phase ratio, and balance the first chromatographic column 5-1 before injection;

[0183] After the above steps are completed, start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve 10 to connect the second chromatographic column 5-2, switch the post-column valve 6 to connect the second chromatographic column 5-2 with the waste liquid tank 9, operate the chromatographic pump according to the time program and the mobile phase ratio, and balance the second chromatographic column 5-2 before injection;

[0184] Step 2, injection of the first chromatographic column 5-1:

[0185] Start the first chromatographic pump 4-1, switch the pre-column valve 10 to connect to the first chromatographic column 5-1, switch the post-column valve 6 to connect the first chromatographic column 5-1 to the waste liquid tank 9, run the chromatographic pump according to the time program, and inject the sample into the first chromatographic column;

[0186] Step 3, elution - washing - balancing of the first chromatographic column 5-1, injection of the second chromatographic column 5-2:

[0187] Start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve 10 to connect the first chromatographic column 5-1, and at the same time, the first chromatographic pump is connected to the second chromatographic column 5-2. Switch the post-column valve 6 to connect the first chromatographic column 5-1 to the detector device 7, collect samples through the fraction collector 8, and at the same time, connect the second chromatographic column 5-2 to the waste liquid tank 9. Run the second chromatographic pump 4-2 and the third chromatographic pump 4-3 according to the time program and the proportion of the mobile phase, and perform elution-column washing-equilibrium on the first chromatographic column 5-1, and at the same time, run the first chromatographic pump to inject samples into the second chromatographic column 5-2.

[0188] Step 4, injecting into the first chromatographic column 5-1, eluting into the second chromatographic column 5-2 - washing the column - balancing:

[0189] Start the second chromatographic pump 4-2 and the third chromatographic pump 4-3, switch the pre-column valve to connect the two chromatographic pumps to the second chromatographic column 5-2, and at the same time connect the first chromatographic pump to the first chromatographic column 5-1. Switch the post-column valve 6 to connect the second chromatographic column 5-2 to the detector device 7, collect samples through the fraction collector 8, and at the same time connect the first chromatographic column 5-1 to the waste liquid tank 9. Run the second chromatographic pump 4-2 and the third chromatographic pump 4-3 according to the time program and the mobile phase ratio, and perform elution-column washing-equilibration on the second chromatographic column 5-2, and at the same time run the first chromatographic pump to inject samples into the first chromatographic column 5-1.

[0190] Step 5, cycle: According to the above two steps, cycle the two chromatographic columns through column equilibration - injection - elution - column washing, and collect samples.

Claims

1. An automated chromatography system with multiple columns in parallel, comprising a sample tank (1), a mobile phase device (2), a multi-way solvent selection valve (3), a chromatography pump (4), a chromatography column device (5), a post-column valve (6), a detector device (7), a fraction collector (8), a waste liquid tank (9) and connecting pipes; It is characterized in that The mobile phase device comprises at least two mobile phase storage tanks arranged in parallel, The number of chromatographic pumps is greater than or equal to 2 and they are connected in parallel. The chromatographic column device comprises at least two identical chromatographic columns arranged in parallel, The detector device comprises at least one detector, The detector device is arranged in parallel with the waste liquid tank (9); After the sample tank (1) and the mobile phase device (2) are connected in parallel, they are connected to a multi-way solvent selection valve (3) through a connecting pipe, and the multi-way solvent selection valve (3) is connected to a chromatographic pump (4) through a connecting pipe; the chromatographic pump (4) is connected to a chromatographic column device (5) through a connecting pipe, and the chromatographic column device (5) is connected to a post-column valve (6) through a connecting pipe, and the post-column valve (6) is connected to a detector device (7) and a waste liquid tank (9) connected in parallel through a connecting pipe, and the detector device (7) is connected to a fraction collector (8) through a connecting pipe.

2. An automated chromatography system with multiple columns in parallel, comprising a sample tank (1), a mobile phase device (2), a chromatography pump (4), a chromatography column device (5), a post-column valve (6), a detector device (7), a fraction collector (8), a waste liquid tank (9), a pre-column valve (10) and connecting pipes; It is characterized in that The mobile phase device comprises at least two mobile phase storage tanks arranged in parallel, The number of chromatographic pumps is greater than or equal to 3, The chromatographic column device comprises at least two identical chromatographic columns arranged in parallel, The detector device comprises at least one detector, The detector device is arranged in parallel with the waste liquid tank; The sample tank (1) is connected to the chromatographic pump (4) through a connecting pipe to form a sample delivery passage; the mobile phase storage tanks arranged in parallel are respectively connected to the chromatographic pump (4) through connecting pipes, and then connected to form a mobile phase delivery passage through connecting pipes; the sample delivery passage and the mobile phase delivery passage are respectively connected to the pre-column valve (10) through connecting pipes; the pre-column valve (10) is connected to the chromatographic column device (5) through a connecting pipe; the chromatographic column device (5) is connected to the post-column valve (6) through a connecting pipe; the post-column valve (6) is connected to the parallel detector device (7) and the waste liquid tank (9) through a connecting pipe; the detector device (7) is connected to the fraction collector (8) through a connecting pipe.

3. The automated chromatography system according to claim 1, It is characterized in that The invention also comprises a pre-column valve (10), wherein the pre-column valve is arranged before the chromatographic pump or the chromatographic column.

4. The automated chromatography system according to any one of claims 1 and 2, It is characterized in that When the detector device includes two or more detectors, the detectors are identical and are arranged in parallel.

5. The automated chromatography system according to any one of claims 1 and 2, It is characterized in that The chromatographic column is selected from a normal phase chromatographic column, a reverse phase chromatographic column, an ion exchange column, and a gel chromatographic column.

6. The automated chromatography system according to any one of claims 1 and 2, It is characterized in that The detector is selected from a UV detector, a conductivity detector, a diode array detector, an evaporative light scattering detector, and a mass spectrometer detector.

7. The automated chromatography system according to any one of claims 1 and 2, It is characterized in that In the gradient mode, the chromatographic pump is selected from one or both of a common pump and a high-precision pump.

8. The automated chromatography system according to claim 1, It is characterized in that In the gradient mode, the automated chromatography system further comprises a proportional valve (11).

9. The automated chromatography system according to claim 8, It is characterized in that In the gradient mode, the gradient elution can be controlled by a proportional valve (11).

10. The automated chromatography system according to claim 2, It is characterized in that In the gradient mode, the method of achieving gradient elution can be controlled by the chromatographic pump (4).

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