Green pesticide for preventing and treating plant bacterial diseases

Abstract

The invention discloses a green pesticide for preventing and treating plant bacterial diseases, which can adhere to the surfaces of various plant leaves and has a protein film with an inhibiting effect on plant bacteria, protein, polyphenol substances and a protein modifier are mixed and stirred uniformly, the mixed solution is sprayed on the plant leaves, and the plant bacterial diseases can be prevented and treated. The unfolded compound of protein and polyphenol is rapidly assembled into an oligomer and forms a compact protein film on plant leaves under the action of surface tension and hydrophobicity, so that the protein film is tightly adhered to the surfaces of the plant leaves, and the current situation that pesticides are difficult to deposit and adhere to the plant leaves at present is greatly improved. And the protein film has an inhibition effect on citrus canker pathogen and cucumber bacterial angular leaf spot pathogen and has good biocompatibility, so that the defects of high toxicity and difficult degradation of pesticides in the current market are changed to a certain extent.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural chemicals, and in particular relates to a green pesticide for preventing and controlling plant bacterial diseases. Background Art

[0002] Bacterial diseases of plants refer to diseases caused by bacterial infection. Bacterial diseases of plants have a wide range of effects and can occur on various vegetables, fruit trees and plants. Severe bacterial diseases can cause deformities, tissue necrosis and rot in various crops. Once these diseases occur, they will seriously hinder the normal growth of various crops and seriously affect the product yields of various crops in my country. For example, when citrus canker is severe, it will cause large-scale leaf and fruit drop in fruit trees, and even destroy the orchard. In addition, bacterial angular leaf spot can cause the death of seedlings or rot of fruits, seriously affecting the yield of cucumbers, causing the cucumber yield to be halved, and in severe cases, it may even lead to a total loss of yield.

[0003] At present, the agents commonly used to prevent and treat bacterial diseases are mainly copper preparations, such as copper hydroxide (inorganic copper) and quinoline copper (organic copper). However, these copper preparations also have many disadvantages in the process of use. Copper ions have good stability and are not easily degraded under natural conditions. The accumulation over the years causes excessive copper in the soil, which can poison the root system of crops and seriously affect crop growth. In addition, the fluidity of copper preparations is poor, and the efficacy deteriorates in humid or rainy weather. They need to be reused. The long-term use of these copper preparations causes plants to develop different degrees of resistance. Therefore, some green pesticides with the advantages of high biological activity, degradability and low residue have emerged. Under this trend, plant extracts have received widespread attention as an important pesticide resource. Its natural, environmentally friendly and green characteristics make it one of the hot spots in current pesticide research and application. So far, nearly 20 plant extracts have been registered, and the pesticide preparations extended from them are as many as nearly 100. Plant extracts can be used to prevent and treat a variety of diseases and insect pests.

[0004] In addition, there are some limitations in the use of pesticides. For example, traditional pesticide droplets are easy to splash on the target leaf surface, and it is difficult to effectively deposit on the leaf surface or form a stable interaction with the leaf surface. These problems have led to a large amount of pesticide waste and reduced the efficacy of pesticides. The current solution is to add polymers or surfactants to pesticides to increase the utilization rate of pesticides, but these substances and their degradation products will cause secondary damage to environmental safety and human health, which does not meet the requirements of sustainable development.

[0005] CN 111802385 A discloses a protein product that can fix a target object and can be removed on demand, which includes protein, protein modifier, and target fixable object. The product uses a protein film generated by protein phase transition to fix the target fixable object on the target surface, thereby improving the utilization rate of the target fixable object and reducing the waste of resources and environmental pollution caused by the loss of the target fixable object. In addition, as the protein film is decomposed, the target fixable object will also be effectively removed. Although the product can use a starch-like system as an adjuvant to improve the utilization rate of pesticides, its function is relatively single and needs to be used in combination with traditional pesticides, which will still cause certain damage to environmental safety and human health. Summary of the invention

[0006] In view of the problems existing in the existing agents for preventing and treating plant bacterial diseases, the present invention provides a green pesticide for preventing and treating plant bacterial diseases which can stably adhere to the surfaces of various plant leaves and has good biocompatibility.

[0007] The green pesticide for preventing and controlling plant bacterial diseases provided by the present invention is liquid or solid; when the green pesticide is liquid, it is composed of protein, polyphenols, protein modifier, pH regulator and distilled water, and the mass ratio of each component in the green pesticide is: 10-600 parts of protein, 10-600 parts of polyphenols, 10-600 parts of protein modifier and 3000-180000 parts of distilled water, and the pH regulator is used to adjust the pH of the green pesticide to 7-11; when the green pesticide is solid, it is composed of protein, polyphenols, protein modifier and pH regulator, and the mass ratio of each component in the green pesticide is: 10-600 parts of protein, 10-600 parts of polyphenols, 10-600 parts of protein modifier and 0.1-6 parts of pH regulator.

[0008] Furthermore, when the green pesticide is in liquid form, the mass ratio of the components is preferably: 100 to 300 parts of protein, 100 parts of polyphenols, 100 to 300 parts of protein modifier, and 30,000 to 90,000 parts of distilled water, and the pH adjuster is used to adjust the pH of the green pesticide to 7 to 11.

[0009] Furthermore, when the green pesticide is solid, the weight ratio of each component is preferably: 100-300 parts of protein, 100 parts of polyphenols, 100-300 parts of protein modifier, and 1-3 parts of pH adjuster.

[0010] Furthermore, the above-mentioned protein is selected from one or more of lysozyme containing disulfide bonds, bovine serum albumin, human serum albumin, lactoferrin, whey albumin, insulin, α-lactalbumin, fibrinogen, β-lactoglobulin, ribonuclease A, cytochrome c, α-amylase, horseradish peroxidase, pepsin, myoglobin albumin, collagen, keratin, hemoglobin, DNA polymerase, casein, soy protein, pea protein isolate, zein, gliadin, gluten, oat protein, potato protein, hemp kernel protein, walnut protein, rapeseed protein, hazelnut protein, quinoa protein, sunflower seed protein, pumpkin seed protein, black sesame protein, mushroom protein, red algae protein, legumin, barley protein, wheat protein, glutenin, kidney bean protein, casein, collagen, catalase, etc.

[0011] Furthermore, the above-mentioned polyphenols are selected from any one or more of gallic acid, caffeic acid, coumaric acid, ferulic acid, protocatechuic acid, cinnamic acid, catechin, epicatechin, gallocatechin, epigallocatechin, quercetin, kaempferol, isorhamnetin, apigenin, luteolin, resveratrol, anthocyanidins, theaflavins, thearubigins, genistein, daidzein, cafestol, kahweol, rutin, hesperidin, gallotannin, ellagitannins, proanthocyanidins, baicalin, etc.

[0012] Furthermore, the above-mentioned protein modifier is a strong reducing agent or a strong oxidizing agent; the strong reducing agent is selected from any one or more of dithiothreitol, β-mercaptoethanol, tris(2-carboxyethyl)phosphine hydrochloride, cysteine, reduced glutathione, ascorbic acid, oxalic acid, amino acids, citric acid, glycyrrhetinic acid, etc., and the strong oxidizing agent is any one or more of ozone, hydrogen peroxide, urea, etc.

[0013] Furthermore, the above-mentioned plants are one or more crops selected from lotus leaves, spinach, lettuce, celery, green vegetables, leeks, rapeseed, cabbage, eggplant, cucumber, citrus, melon, watermelon, loofah, cantaloupe, tomato, pepper, cabbage, potato, corn, wheat and rice.

[0014] Furthermore, when the green pesticide of the present invention is liquid, the pH adjuster is NaOH or hydrochloric acid; when the green pesticide of the present invention is solid, the pH adjuster is any one or more of NaOH, sodium carbonate, sodium bicarbonate, potassium bicarbonate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, potassium benzoate, sodium citrate, etc.

[0015] Furthermore, when the green pesticide of the present invention is in liquid form, it must be prepared and used immediately. When used, the protein modifier, protein, and polyphenols are added to water, mixed evenly, and the pH is adjusted to 7 to 11 with a pH adjuster, and then sprayed onto the surface of plants to form a protein film on the surface of the plants.

[0016] Furthermore, when the green pesticide of the present invention is solid, the green pesticide is added into water at a ratio of 5 to 10 g / L and mixed evenly, and then sprayed onto the surface of the plant to form a protein film on the surface of the plant.

[0017] The green pesticide of the present invention can prevent and control various bacterial diseases, for example: rice bacterial leaf blight, rice bacterial stripe, rice base rot, rice bacterial brown stripe, rice bacterial brown spot, corn bacterial stem rot, corn bacterial leaf spot, wheat bacterial stripe, wheat bacterial leaf blight, potato bacterial wilt, potato ring rot, potato soft rot, potato black shank, soybean bacterial spot, peanut bacterial wilt, peanut bacterial leaf spot, tomato bacterial wilt, tomato canker, tomato bacterial spot, cucumber bacterial angular spot, cucumber bacterial leaf blight, pepper bacterial wilt, pepper bacterial leaf spot, eggplant bacterial wilt, cabbage soft rot, carrot soft rot, citrus canker, peach bacterial perforation, apple bacterial leaf blight, grape bacterial angular spot, strawberry bacterial wilt and the like.

[0018] The beneficial effects of the present invention are as follows:

[0019] 1. The present invention provides a simple, rapid and mild protein interface self-assembly process to construct a protein nanofilm on the plant surface. Because a large number of active groups are exposed on the protein surface after phase transition, these active groups can interact with the plant surface through coordination bonds and hydrogen bonds, thereby achieving stable adhesion on the plant surface. In addition, proteins with a large number of active groups can interact with polyphenols, so that the protein-polyphenol composite film exhibits strong adhesion and has a good preventive and therapeutic effect on plant bacterial diseases.

[0020] 2. The present invention utilizes natural substances such as proteins and polyphenols to prevent and treat plant bacterial diseases, which has the advantages of high efficiency, non-toxicity, low environmental pollution, and low use cost, and can effectively reduce the use of substances such as copper preparations and antibiotics in the field.

[0021] 3. The present invention uses the excellent adhesion properties of the protein phase transition system and adds gallic acid, a natural active ingredient with strong antibacterial properties, to the phase system to prepare a protein and polyphenol composite film to prevent and control bacterial diseases in agriculture. The composite film preparation method is gentle and controllable. The present invention can control the thickness of the protein film by regulating the concentration of the protein modifier, protein and polyphenols, the reaction time, the number of spraying times and other conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Thickness statistics of protein films prepared at different reaction times in Example 1.

[0023] Figure 2The adhesion of the protein film prepared by reacting for 180 min in Example 1 on the surface of the silicon substrate was measured using a nano scratch tester.

[0024] Figure 3 is the contact angle of the protein film in Example 2 on the surfaces of various plant leaves.

[0025] Figure 4 This is a comparison of the protein film coverage area on the lotus leaf surface before and after rain erosion in Example 2.

[0026] Figure 5 It is the inhibition rate of the pesticides with different concentrations of gallic acid on cucumber bacterial angular leaf spot in Example 3.

[0027] Figure 6 It is the inhibition rate of the pesticides with different concentrations of gallic acid on citrus canker bacteria in Example 3.

[0028] Figure 7 This is the control effect on bacterial angular leaf spot of cucumber in Experiment 1.

[0029] Figure 8 is the cell viability of each sample in Experiment 2.

[0030] Fig. 9 is the mortality rate of earthworms in each sample in Test 3.

[0031] Fig.10 This is a graph showing the weight changes of earthworms of each sample in Experiment 3. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention is not limited to these embodiments.

[0033] Example 1

[0034] At room temperature, 10 mL of 10 mg / mL lysozyme aqueous solution, 10 mL of 10 g / mL gallic acid aqueous solution and 10 mL of 50 mM tris(2-carboxyethyl)phosphine aqueous solution were mixed, and the pH was adjusted to 7 using NaOH. A silicon wafer with a length and width of 1 cm was incubated upside down on the mixed solution for different periods of time, and then washed with deionized water and dried to obtain a silicon substrate loaded with a protein film. Figure 1 It can be seen that within the reaction time of 180 min, the thickness of the protein film increased continuously with the extension of the reaction time, and then remained unchanged. Figure 2 It is proved that the adhesion force of the protein film prepared by reaction for 180 minutes can reach 1840.50μN.

[0035] Example 2

[0036] At room temperature, 10 mL of 10 mg / mL lysozyme aqueous solution, 10 mL of 10 g / mL gallic acid aqueous solution and 10 mL of 50 mM tri(2-carboxyethyl)phosphine aqueous solution were mixed, and the pH was adjusted to 7 using NaOH. The mixed solution was sprayed on the surface of washed plant leaves using a spray gun and incubated for 3 hours. It was then washed with deionized water and dried to obtain plant leaves loaded with protein films.

[0037] pass Figure 3 The change in contact angle proves that the protein film can adhere to the surfaces of lotus leaves, citrus, cucumber, eggplant, Chinese cabbage, rapeseed, spinach and lettuce, proving the universality of the protein film. Figure 4 It can be seen that after the lotus leaf surface is washed by rainwater, the coverage rate of the protein film decreases less and can still reach more than 20%, indicating that the protein film has good resistance to rainwater erosion.

[0038] Example 3

[0039] At room temperature, 10 mL of 10 mg / mL lysozyme aqueous solution, 10 mL of different concentrations of gallic acid aqueous solution (gallic acid concentrations are 0.5, 1, 2, 3, 4, 5 mg / mL) and 10 mL of 50 mM tris(2-carboxyethyl)phosphine aqueous solution were mixed, and the pH was adjusted to 7 with NaOH to obtain pesticides with different concentrations of gallic acid. Accurately measure 1 mL of pesticide and mix it with 9 mL of nutrient agar medium cooled to 50 ° C and sterilized, mix well and pour into a sterilized culture dish, and after cooling, it is a drug-containing culture medium plate. The cucumber bacterial angular spot pathogen and citrus canker pathogen were diluted to different concentrations, and then inoculated on the drug-containing culture medium plate, and their growth inhibition rate was calculated after incubation for 48 hours. The concentrations of gallic acid calculated here are all final concentrations. For example, after mixing at a volume ratio of 1:9, the concentration of gallic acid becomes 0.1 times of the original concentration, which are 16.7, 33.3, 66.7, 100, 133.3 and 166.7 μg / mL, respectively. Meanwhile, a control experiment was performed without adding gallic acid.

[0040]

[0041] Depend on Figure 5 and Figure 6 It can be seen that this product has a good antibacterial effect, and with the increase of gallic acid concentration, its inhibition rate against citrus canker bacteria and cucumber bacterial angular spot bacteria gradually increases.

[0042] In order to prove the beneficial effects of the present invention, the inventors further conducted the following experiments:

[0043] Experiment 1: Control of bacterial angular leaf spot of cucumber in actual farmland.

[0044] The copper quinoline suspension with a mass concentration of 33.5% (preparation amount 1.8 mL, liquid volume 1349 mL, wherein the preparation amount is the added pesticide, and the liquid volume is the total volume after the pesticide is added), the copper hydroxide water dispersible granules with a mass concentration of 46% (preparation amount 0.9 mL, liquid volume 1349 mL), the phase transition lysozyme solution (10 mg / mL lysozyme aqueous solution, 2 mg / mL pH = 7 gallic acid aqueous solution, and water are mixed in a volume ratio of 1:1:1), 667 μg / mL gallic acid aqueous solution and the experimental sample (at room temperature, 10 mL 10 mg / mL lysozyme aqueous solution, 10 mL 2 mg / mL gallic acid aqueous solution and 10 mL 50mM tri(2-carboxyethyl)phosphine aqueous solution was mixed and the pH was adjusted to 7 with NaOH) and sprayed on the cucumber test field. Disease level survey: five random sampling points were surveyed in each plot, and all leaves of three plants were surveyed at each point. Each leaf was graded and recorded according to the percentage of lesions in the leaf area. The grading standard is: Grade 0: no lesions; Grade 1: the lesion area accounts for less than 5% of the entire leaf area; Grade 3: the lesion area accounts for less than 6% to 10% of the entire leaf area; Grade 5: the lesion area accounts for less than 11% to 25% of the entire leaf area; Grade 7: the lesion area accounts for less than 26% to 50% of the entire leaf area; Grade 9: the lesion area accounts for more than 50% of the entire leaf area. The disease index and control effect were calculated according to the following formula.

[0045]

[0046] Wherein, CK0 is the disease index of the blank control area before application of pesticides; CK1 is the disease index of the blank control area after application of pesticides; PT0 is the disease index of the drug-treated area before application of pesticides; PT1 is the disease index of the drug-treated area after application of pesticides.

[0047] Depend on Figure 7 It can be seen that the experimental samples have a better inhibitory effect on cucumber bacterial angular leaf spot than phase-shift lysozyme and gallic acid alone, and their control effect is also better than commercial pesticides 33.5% quinoline copper and 46% copper hydroxide.

[0048] Experiment 2: Cell biocompatibility of protein films

[0049] At room temperature, 10 mL of 10 mg / mL lysozyme aqueous solution, 10 mL of 2 g / mL gallic acid aqueous solution, and 10 mL of 50 mM tri(2-carboxyethyl)phosphine aqueous solution were mixed in equal volumes, and the pH was adjusted to 7 using NaOH. 1 mL of the mixed solution was dropped onto a glass slide with a length of 6 cm and a width of 1 cm and incubated for 3 hours to prepare a protein film of equal area. Human embryonic lung fibroblasts (IMR90) were used to test the biocompatibility of the protein film. All operations were completed in a sterile clean bench. First, the protein film was placed under ultraviolet light for 30 minutes to achieve the purpose of sterilization. And according to the 6 cm 2 The protein film was completely immersed in 0.9% physiological saline for 24 hours at a ratio of 1:1 / 10 / mL to obtain an extract of the protein film. The extract was diluted stepwise to 1 / 2, and after culturing for 24 hours, its absorbance was measured at a wavelength of 450 nm using an ELISA reader.

[0050] Depend on Figure 8 It can be seen that compared with the blank group, the cell survival rate is not much different, so its biocompatibility is better.

[0051] Experiment 3: Biocompatibility of protein films

[0052] Ten Eisenia fetida earthworms were placed in earthworm culture boxes with soil at the bottom and air holes at the top (the weight of Eisenia fetida earthworms was 0.3-0.6 g, and the soil in each culture box was about 500 g). 1 mL of pure water and 1 mL of experimental sample (mixed 10 mL of 10 mg / mL lysozyme aqueous solution, 10 mL of 2 mg / mL gallic acid aqueous solution and 10 mL of 50 mM tris(2-carboxyethyl)phosphine aqueous solution at room temperature, and the pH was adjusted to 7 with NaOH) were added to each culture box in turn. The culture was carried out for 14 days at a temperature of 20°C, a humidity of 80%, and a light of 600 lx. The soil was poured out from the bottle at 7 days, 10 days, and 14 days, and the number of dead earthworms (touch the tail of the earthworm with a needle tip, if the earthworm does not respond, it is dead) and the weight change at 0 days, 7 days, 10 days, and 14 days were counted. The survival rate formula is as follows, where N is the number of remaining earthworms, M is the total number of earthworms, and three parallel samples are made for each group of experiments.

[0053]

[0054] Depend on Fig. 9 It can be seen that the survival rate of earthworms is not much different from that of the blank group. Fig.10 It can be seen that compared with the blank control group, the average weight of earthworms did not change much with the number of days, which can prove that its biocompatibility is good.

Claims

1. A green pesticide for preventing and controlling plant bacterial diseases, characterized in that: The green pesticide is liquid or solid; When the green pesticide is liquid, it is composed of protein, polyphenols, protein modifier, pH regulator, and distilled water, and the mass ratio of each component in the green pesticide is: 10 to 600 parts of protein, 10 to 600 parts of polyphenols, 10 to 600 parts of protein modifier, and 3,000 to 180,000 parts of distilled water, and the pH regulator is used to adjust the pH of the green pesticide to 7 to 11; When the green pesticide is solid, it is composed of protein, polyphenols, protein modifiers, and pH regulators, and the mass ratio of each component in the green pesticide is: 10 to 600 parts of protein, 10 to 600 parts of polyphenols, 10 to 600 parts of protein modifiers, and 0.1 to 6 parts of pH regulators.

2. The green pesticide for preventing and controlling plant bacterial diseases according to claim 1, characterized in that: When the green pesticide is liquid, the mass proportions of the components are: 100-300 parts of protein, 100 parts of polyphenols, 100-300 parts of protein modifier, 30,000-90,000 parts of distilled water, and the pH regulator is used to adjust the pH of the green pesticide to 7-11.

3. The green pesticide for preventing and controlling plant bacterial diseases according to claim 1, characterized in that: When the green pesticide is solid, the mass proportions of the components are: 100-300 parts of protein, 100 parts of polyphenols, 100-300 parts of protein modifier, and 1-3 parts of pH regulator.

4. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: The protein is selected from any one or more of lysozyme, bovine serum albumin, human serum albumin, lactoferrin, whey albumin, insulin, α-lactalbumin, fibrinogen, β-lactoglobulin, ribonuclease A, cytochrome c, α-amylase, horseradish peroxidase, pepsin, myoglobin albumin, collagen, keratin, hemoglobin, DNA polymerase, casein, soy protein, pea protein isolate, zein, gliadin, gluten, oat protein, potato protein, hemp seed protein, walnut protein, rapeseed protein, hazelnut protein, quinoa protein, sunflower seed protein, pumpkin seed protein, black sesame protein, mushroom protein, red algae protein, legumin, barley protein, wheat protein, glutenin, kidney bean protein, casein, collagen, and catalase.

5. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: The polyphenols are selected from any one or more of gallic acid, caffeic acid, coumaric acid, ferulic acid, protocatechuic acid, cinnamic acid, catechin, epicatechin, gallocatechin, epigallocatechin, quercetin, kaempferol, isorhamnetin, apigenin, luteolin, resveratrol, anthocyanidins, theaflavins, thearubigins, genistein, daidzein, cafestol, kahweol, rutin, hesperidin, gallotannins, ellagitannins, proanthocyanidins, and baicalin.

6. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: The protein modifier is a strong reducing agent or a strong oxidizing agent; the strong reducing agent is any one or more of dithiothreitol, β-mercaptoethanol, tris(2-carboxyethyl)phosphine hydrochloride, cysteine, reduced glutathione, ascorbic acid, oxalic acid, amino acids, citric acid, and glycyrrhetinic acid; the strong oxidizing agent is any one or more of ozone, hydrogen peroxide, and urea.

7. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: When the green pesticide is liquid, the pH adjuster is NaOH or hydrochloric acid; when the green pesticide is solid, the pH adjuster is any one or more of NaOH, sodium carbonate, sodium bicarbonate, potassium bicarbonate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, potassium benzoate, and sodium citrate.

8. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: The plant is any one or more of lotus leaf, spinach, lettuce, celery, green vegetables, leek, rape, cabbage, eggplant, cucumber, citrus, melon, watermelon, loofah, cantaloupe, tomato, pepper, cabbage, potato, corn, wheat and rice.

9. The green pesticide for preventing and controlling plant bacterial diseases according to any one of claims 1 to 3, characterized in that: The bacterial diseases are any one or more of rice bacterial leaf blight, rice bacterial streak, rice basal rot, rice bacterial brown streak, rice bacterial brown spot, corn bacterial stem rot, corn bacterial leaf spot, wheat bacterial streak, wheat bacterial leaf blight, potato bacterial wilt, potato ring rot, potato soft rot, potato black shank, soybean bacterial leaf spot, peanut bacterial wilt, peanut bacterial leaf spot, tomato bacterial wilt, tomato canker, tomato bacterial leaf spot, cucumber bacterial angular spot, cucumber bacterial leaf blight, pepper bacterial wilt, pepper bacterial leaf spot, eggplant bacterial wilt, cabbage soft rot, carrot soft rot, citrus canker, peach bacterial punchhole, apple bacterial leaf blight, grape bacterial angular spot, strawberry bacterial wilt.

10. The green pesticide for preventing and controlling plant bacterial diseases according to claim 1 or 2, characterized in that: When the green pesticide is in liquid form, it must be prepared and used immediately. When used, the protein modifier, protein, and polyphenols are added to water, mixed evenly, and the pH is adjusted to 7 to 11 with a pH adjuster, and then sprayed onto the plant surface to form a protein film on the plant surface; When the green pesticide is solid, the green pesticide is added into water at a ratio of 5 to 10 g / L and mixed evenly, and then sprayed onto the surface of the plant to form a protein film on the surface of the plant.

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