Pesticide preparation and method for promoting yield increase and branching of medicago sativa

Abstract

The invention relates to the technical field of plant growth regulation, in particular to a pesticide preparation and method for promoting yield increase and branching of medicago sativa L. The pesticide preparation is prepared from, by weight, 1-4 parts of benayl aminopurine, 0.1 part of compound sodium nitrophenolate, 1-4 parts of brassinolide, 1-3 parts of triacontanol and 1-4 parts of uniconazole. When the medicago sativa grows to a seedling stage after being sown, the pesticide preparation is applied after the medicago sativa is cut on the ground, branching of the medicago sativa can be effectively promoted, the yield of the medicago sativa is increased, and the quality of the medicago sativa cannot be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of plant growth regulation, in particular to a pesticide preparation and a method for promoting the yield increase and branching of alfalfa. Background Art

[0002] Alfalfa is a high-quality and high-yield forage grass, known as the "king of forage grasses". It has a high protein content and is an important source of feed.

[0003] In recent years, with the improvement of people's living standards, the demand for meat and dairy products has increased, and the demand for high-quality forage has also increased. Research on alfalfa yield increase technology to meet domestic market demand and reduce the import of high-quality forage has important practical significance for the development of domestic animal husbandry. Therefore, how to increase alfalfa yield has always been a hot topic in forage research.

[0004] At present, the increase of alfalfa production is often promoted by applying plant growth regulators, improving fertilization methods and strengthening field management measures. However, the application of plant growth regulators has the problem of having to re-apply after each harvest, which increases the cost; improving fertilization methods and strengthening field management measures will increase the workload of daily management of alfalfa planting, requiring a lot of manpower and material resources to ensure the effect of increasing alfalfa production, and it is difficult to implement on a large scale in areas with large alfalfa planting areas. Summary of the invention

[0005] In order to solve the defects in the prior art, the present invention provides a pesticide preparation and method for promoting alfalfa yield increase and branching. The method of the present invention can effectively promote alfalfa branching and increase alfalfa yield without causing quality decline.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a pesticide preparation for promoting the increase of yield and branching of alfalfa. The ingredients of the pesticide preparation, measured by weight, include 1 to 4 parts of benzylaminopurine, 0.1 part of sodium nitrophenolate, 1 to 4 parts of brassinolide, 1 to 3 parts of triacontanol, and 1 to 4 parts of uniconazole.

[0008] Preferably, the ingredients of the pesticide preparation, measured in parts by weight, include 2 parts of benzylaminopurine, 0.1 parts of sodium nitrophenolate, 2 parts of brassinolide, 2 parts of triacontanol, and 2 parts of uniconazole.

[0009] In a second aspect, the present invention provides a method for promoting the yield and branching of alfalfa, comprising: applying the pesticide formulation after mowing the alfalfa evenly when it grows to the seedling stage after sowing.

[0010] Preferably, the pesticide formulation comprises 2.5×10 benzylaminopurine5 ~10 6 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 2.5×10 5 ~10 6 times solution, triacontanol 3.3×10 5 ~10 6 times liquid, uniconazole 2.5×10 5 ~10 6 times liquid.

[0011] Preferably, the pesticide formulation includes 5×10 benzylaminopurine 5 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 5×10 5 times solution, triacontanol 5×10 5 times liquid, 5×10 5 times liquid.

[0012] Preferably, the alfalfa is mowed flush with the ground at the seedling stage 20 days after sowing.

[0013] Preferably, the application amount of the pesticide preparation is 100 mL / plant.

[0014] Preferably, the application site of the pesticide preparation is the above-ground part of alfalfa seedlings.

[0015] Preferably, the branching position is a cutting cut.

[0016] Preferably, the alfalfa is harvested three times, with the first harvest being 50 days after sowing, the second harvest being 35 days after the first harvest, and the third harvest being 35 days after the second harvest.

[0017] Preferably, the stubble height after the three mowings is 5 cm.

[0018] The beneficial effects of the present invention are:

[0019] The pesticide preparation used in the invention is helpful to promote the branching of alfalfa, improve the yield of alfalfa, and will not cause the decrease of the crude protein content, crude fiber and relative feeding value of alfalfa.

[0020] In the method for promoting the increase of alfalfa yield and branching of the present invention, a combination of physical measures and the application of a regulator is adopted, and when the alfalfa grows to the seedling stage, the alfalfa is first mowed evenly and then a pesticide preparation is applied. In this process, the apical dominance of the alfalfa seedlings can be removed after mowing evenly during the alfalfa seedling stage, and the pesticide preparation is then used to promote the alfalfa to sprout more branches. The branches at this time can gradually grow into thick and strong branches as the alfalfa seedlings grow, thereby achieving the purpose of increasing the yield. After adopting the method of the present invention, the yield, crude protein, crude fiber and relative feeding value of alfalfa are measured for two consecutive years. The results show that the method can significantly affect the yield of alfalfa after promoting the branching of alfalfa, and the yield-increasing effect is good, and the crude protein and crude fiber content and relative feeding value are not significantly affected, and the quality will not be reduced.

[0021] The invention only processes alfalfa in the seedling stage during the sowing period to promote branch formation. The tillering ability of cells in the seedling stage is the strongest. As the branches of alfalfa grow more and more mature, the tillering ability is greatly weakened, and there is no need to repeatedly apply the agent after harvesting. The invention can greatly reduce the pressure of alfalfa field management, reduce labor and save costs, and has high economic value. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of setting up a test site for an embodiment of the present invention;

[0023] Figure 2 This is a picture of the test sample area before sowing in the embodiment of the present invention;

[0024] Figure 3 This is a schematic diagram of the hole planting in the test sample area of ​​the embodiment of the present invention;

[0025] Figure 4 This is a picture of the test sample area after sowing in the embodiment of the present invention;

[0026] Figure 5 This is a diagram of the branching of seedlings before mowing and after mowing and medication. DETAILED DESCRIPTION

[0027] In order to enable those skilled in the art to better understand the technical solution of the invention, the present invention is further described in detail below in conjunction with specific implementation methods.

[0028] Example

[0029] Experimental plot setting: The experiment was conducted on April 20, 2020 in the experimental field of Guoguang Garden Technology Co., Ltd. The average temperature of the greenhouse was 25°C and the humidity was 54%. Spring sowing of alfalfa Gannong No. 1 seeds was carried out, and 4 experimental plots were set up (see the setting method for details). Figure 1, respectively corresponding to Examples 1 to 4), each test plot is 10m×10m, each plot is 2m apart, the soil matrix is ​​loam, and the test plot before sowing is shown in the picture Figure 2 .

[0030] Hole sowing: After the land is leveled, hole sowing is carried out with a hole spacing of 50 cm, a total of 121 holes (see Figure 3 As shown in the figure, there are 484 sowing holes in 4 sample plots, each hole is 2cm deep, 5 seeds are placed in each sowing hole, and the soil is covered with 5mm. During the seed germination period, water is applied once a day at 9 am to ensure water supply. On May 5, 15 days after alfalfa sowing, one alfalfa plant is retained in each sowing hole. If there are too many alfalfa plants in the sowing hole, they are thinned out. If there are sowing holes that have not sprouted, they are transplanted to other sowing holes. Finally, 100 sowing holes are retained in each sample plot, and the sowing holes are numbered in a certain row and column. The pictures of the test sample plots after sowing are shown in the figure. Figure 4 .

[0031] Application of pesticide after cutting seedlings: The test plots of Examples 1 to 4 were sprayed after cutting them evenly. The spraying site was the above-ground part of the alfalfa seedlings, and the spraying amount was 100 mL / plant. The spraying treatment of the test plots of Examples 1 to 4 was as follows:

[0032] Embodiment 1:

[0033] Treatment period: On May 10, 20-day-old alfalfa seedlings were mowed flush with the ground and then sprayed;

[0034] Treatment agent: Mix 1g of benzylaminopurine, 0.1g of sodium nitrophenolate, 1g of brassinolide, 1g of triacontanol, and 1g of uniconazole, add to a beaker containing 100ml of water, then take 1ml and add to a sprayer containing 10L of water for spraying (10g of benzylaminopurine). 6 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 10 6 times liquid, triacontanol 10 6 Times liquid, uniconazole 10 6 times liquid).

[0035] Embodiment 2:

[0036] Treatment period: On May 10, 20-day-old alfalfa seedlings were mowed flush with the ground and then sprayed;

[0037] Treatment agent: Mix 2g of benzylaminopurine, 0.1g of sodium nitrophenolate, 2g of brassinolide, 2g of triacontanol, and 2g of uniconazole, add to a beaker containing 100ml of water, then take 1ml and add to a sprayer containing 10L of water for spraying (5×10 benzylaminopurine 5 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 5×105 times solution, triacontanol 5×10 5 times liquid, 5×10 5 times liquid).

[0038] Embodiment 3:

[0039] Treatment period: On May 10, 20-day-old alfalfa seedlings were mowed flush with the ground and then sprayed;

[0040] Treatment agent: Mix 4g of benzylaminopurine, 0.1g of sodium nitrophenolate, 4g of brassinolide, 3g of triacontanol, and 4g of uniconazole, add to a beaker containing 100ml of water, then take 1ml and add to a sprayer containing 10L of water for spraying (2.5×10 benzylaminopurine 5 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 2.5×10 5 times solution, triacontanol 3.3×10 5 times liquid, uniconazole 2.5×10 5 times liquid).

[0041] Embodiment 4:

[0042] Treatment period: On May 10, 20-day-old alfalfa seedlings were mowed flush with the ground and then sprayed;

[0043] Treatment agent: 10L clean water.

[0044] Comparative Example 1

[0045] According to the method of the embodiment, test sample areas with the same conditions were selected for hole sowing, and the agent of Example 2 was used for spraying, with the only difference being that the alfalfa seedlings grown for 20 days after sowing were not mowed flush with the ground, but were directly sprayed.

[0046] The branching conditions and various physiological indices of the alfalfa plants were recorded 0 days, 5 days, and 10 days after the pesticide treatment in the examples and comparative examples, as well as after 3 cuts were cut at the harvest time, see effect examples 1 to 4.

[0047] Effect Example 1 Determination of the number of branches of alfalfa

[0048] The number of branches at 0 days, 5 days and 10 days after the pesticide treatment of the embodiment and the comparative example was recorded, and the number of branches of 100 alfalfa plants after 3 cuts were cut evenly at the harvest period, and the branch position was the cutting cut. The three harvesting periods were specifically as follows: spring alfalfa was sown on April 20, the first cut was 50 days apart, and the harvest date was June 10; the second cut was 35 days apart, and the harvest date was July 15; the third cut was harvested at an interval of 35 days, and the date was August 20, and the stubble height of each cut was 5 cm. The measurement results are shown in Table 1.

[0049] Table 1. Results of determination of branch number of alfalfa

[0050]

[0051]

[0052] The results in Table 1 show that, during the sowing period, the method of spraying after mowing the seedlings flush with the ground significantly increased the number of branches of alfalfa. Example 1 had an average of 4.36 branches per plant at 0 days, 5.32 branches per plant after 5 days, an increase of 22.02%; 5.5 branches per plant after 10 days, an increase of 3.38% relative to 5 days. Example 2 had an average of 4.62 branches per plant at 0 days, 6.73 branches per plant after 5 days, an increase of 45.67%; 6.82 branches per plant after 10 days, an increase of 1.34% relative to 5 days. Example 3 had an average of 4.47 branches per plant at 0 days, 5.53 branches per plant after 5 days, an increase of 23.71%; 5.62 branches per plant after 10 days, an increase of 1.63% relative to 5 days. In Example 4, the average number of branches was 4.26 / plant at 0 day, 4.29 / plant after 5 days, an increase of 0.71%; and 4.33 / plant after 10 days, an increase of 0.93% compared with 5 days. The effect of Example 2 in promoting alfalfa branching was significantly higher than that of other treatments, and the effect of promoting branching was mainly concentrated in the first 5 days, after which only a few new branches grew, and the effect of promoting branching gradually weakened and disappeared.

[0053] Compared with the results of comparative example 1, the effect of using the medicine after mowing in Example 2 is significantly higher than that without mowing. The increase is 21.3% on day 0, 69.5% after 5 days of medication, and 65.1% after 10 days of medication. The effect of using the medicine after mowing is obvious.

[0054] Figure 5 The diagram shows the branching of the seedlings before mowing (left picture) and after mowing and applying the drug (right picture). The drug applied is the drug described in Example 2. It can be seen from the figure that the alfalfa seedlings before mowing have fewer branches and grow around, while the seedlings that grow again after mowing and applying the drug have more branches and grow obliquely upward.

[0055] Effect Example 2 Determination of Alfalfa Yield

[0056] The yield of alfalfa after three mowings at the harvest time was recorded (at this time one-third of the alfalfa was in bloom). The three harvesting periods were the same as in Example 1. The aboveground yield of each alfalfa crop was measured after mowing. All samples were taken from each sample plot, and the aboveground yield was measured and converted into kg / mu. The measurement was continued for two years, three times each year, and the average value was calculated. The results are shown in Table 2.

[0057] Table 2 Alfalfa yield per crop from 2020 to 2021

[0058]

[0059] As can be seen from Table 2, compared with the control group sprayed with clear water in Example 4, the alfalfa yield per crop of Examples 1 to 3 using the agent of the present invention is higher, and the alfalfa yield of Example 2 is significantly higher than that of Examples 1 and 3. In the data of alfalfa yield measured in 2020, in the first crop, the overall yield was not high, and the alfalfa yield of the pesticide treatment was significantly higher than that of the control treatment. Compared with Example 4, Example 1 significantly increased production by 29.54%, Example 2 significantly increased production by 65.24%, and Example 3 significantly increased production by 48.58%; in the second crop, Example 2 and Example 3 were significantly higher than other embodiments, compared with Example 4, Example 1 increased production by 13.88%, Example 2 significantly increased production by 43.07%, and Example 3 significantly increased production by 27.11%; in the third crop, compared with Example 4, Example 1 increased production by 8.85%, with a small increase, Example 2 significantly increased production by 42.13%, and Example 3 significantly increased production by 16.65%. In the data of alfalfa yield measured in 2021, when harvesting the first crop, compared with Example 4, Example 1 increased production by 21.60%, Example 2 increased production by 39.41%, and Example 3 increased production by 13.57%; when harvesting the second crop, compared with Example 4, Example 1 increased production by 25.87%, Example 2 increased production by 45.86%, and Example 3 increased production by 17.53%; when harvesting the third crop, compared with Example 4, Example 1 increased production by 25.47%, Example 2 increased production by 59.96%, and Example 3 increased production by 21.41%.

[0060] Compared with comparative example 1, the yield of alfalfa treated with medicine after mowing was significantly higher than that treated without mowing. In 2020, the yield of the first crop increased by 83.1%, the yield of the second crop increased by 57.7%, and the yield of the third crop increased by 55.1%. In 2021, the yield of the first crop increased by 58.3%, the yield of the second crop increased by 61.1%, and the yield of the third crop increased by 77.2%, with significant increases.

[0061] Effect Example 3 Determination of crude protein content of alfalfa

[0062] The crude protein content was determined by the Kjeldahl method. Ten alfalfa plants were randomly selected in each row for sampling (three mowings were performed during the harvest period, and the period of three mowings was the same as in Example 1). After sampling, they were placed in an oven at 105°C for 30 minutes to kill green, and then dried at 80°C to constant weight. After drying, it was ground into grass powder with a grinder, and 0.5g of grass sample was taken, placed in a digestion tube, and a catalyst was added. After concentrated sulfuric acid was placed in a Kjeldahl nitrogen analyzer for digestion, and after digestion was completed and cooled, it was titrated with hydrochloric acid.

[0063]

[0064] Where:

[0065] V2 : The volume of hydrochloric acid standard titration liquid consumed by the titration sample, in milliliters (ml);

[0066] V 1 : The volume of the standard hydrochloric acid solution consumed in the titration blank, in milliliters (ml);

[0067] c: concentration of hydrochloric acid standard titration solution, in moles per liter (mol / L);

[0068] m: sample mass, in grams (g);

[0069] V: total volume of sample digestion solution, in milliliters (ml);

[0070] V': volume of digester liquid for distillation, in milliliters (ml);

[0071] 14: Molar mass of nitrogen in grams per mole (g / mol);

[0072] 6.25: Average conversion factor of nitrogen to crude protein.

[0073] For each sample, two parallel samples were taken for measurement, and the arithmetic mean value was taken as the measurement result. See Table 3.

[0074] Table 3. Test results of crude protein content of alfalfa

[0075]

[0076] As can be seen from Table 3, there is no significant difference in the crude protein content of alfalfa in each embodiment and the comparative example, indicating that the method of applying pesticides after mowing the seedlings at the sowing stage in the present invention will not cause a reduction in the quality of alfalfa.

[0077] Effect Example 4 Alfalfa crude fiber and relative feeding value

[0078] Test samples: Samples were prepared from three crops of alfalfa plants cut evenly at the harvest time. The specific cutting periods of the three crops were the same as those in Effect Example 1.

[0079] Determination of crude fiber: Alfalfa crude fiber includes acid detergent fiber ADF and neutral detergent fiber NDF. The content of acid detergent fiber (NY / T 1459-2022) and neutral detergent fiber (GB / T 20806-2022) are determined by the filter bag method. The acid detergent fiber is weighed (accurate to 0.001g) after the filter bag is dried at 80℃ for 2h and cooled to room temperature in a dryer. Weigh 0.5g of sample (accurate to 0.001g) in the filter bag and seal the filter bag with a sealing machine. The sample volume does not exceed 1 / 2 of the filter bag capacity. Put the filter bag containing the sample into the digestion device, add acid detergent solution, and heat the sample solution to boiling within 5min. Keep the solution boiling and continue digestion for 1h. After digestion, wash and dry to measure the weight. The determination of neutral detergent fiber is similar to that of acid detergent fiber. The only difference is that neutral detergent solution is added for digestion.

[0080]

[0081] Where:

[0082] m 1 : Sample mass, in grams (g);

[0083] m 2 : Mass of filter bag for blank test, in grams (g);

[0084] m 3 : The mass of the blank test filter bag after digestion and drying, in grams (g);

[0085] m 4 : Sample measurement filter bag mass, in grams (g);

[0086] m 5 : The total mass of the sample and filter bag after digestion and drying, in grams (g).

[0087] Determination of relative feeding value: Relative feeding value is an important indicator of alfalfa value and the main factor affecting livestock feeding. The higher the relative feeding value of the forage, the higher the livestock feed on this type of forage, and the more nutrients they take in. Relative feeding value is related to the content of neutral detergent fiber and acid detergent fiber.

[0088] Dry matter intake (DMI): DMI = 120 / NDF;

[0089] Digestible dry matter (DDM): DDM = 88.9-0.779 × ADF;

[0090] Relative feeding value (RFV): RFV = DMI × DDM / 1.29.

[0091] The sampling and measurement results in 2020 and 2021 are shown in Tables 4 and 5.

[0092] Table 4. Alfalfa detergent fiber content and relative feeding value in 2020

[0093]

[0094] Table 5. Alfalfa detergent fiber content and relative feeding value in 2021

[0095]

[0096] The results in Table 4 show that there is no significant difference in the acid detergent fiber and neutral detergent fiber content and relative feeding value of alfalfa plants after three cuts at the harvest time in 2121. The results in Table 5 show that the acid detergent fiber and neutral detergent fiber content and relative feeding value of three cuts of alfalfa in 2121 are slightly different. The above shows that the treatment method of spraying the seedlings after cutting during the sowing period of the present invention will not reduce the relative feeding value of alfalfa. Among them, the acid detergent fiber and neutral detergent fiber content in Example 2 is lower than that in other embodiments, and the average relative feeding value is higher than that in other embodiments.

[0097] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A pesticide preparation for promoting the increase of alfalfa yield and branching, characterized in that: The ingredients of the pesticide preparation, measured by weight, include 1 to 4 parts of benzylaminopurine, 0.1 part of sodium nitrophenolate, 1 to 4 parts of brassinolide, 1 to 3 parts of triacontanol, and 1 to 4 parts of uniconazole.

2. The pesticide formulation according to claim 1, characterized in that The ingredients of the pesticide preparation are measured by weight and include 2 parts of benzylaminopurine, 0.1 parts of sodium nitrophenolate, 2 parts of brassinolide, 2 parts of triacontanol, and 2 parts of uniconazole.

3. A method for promoting alfalfa yield and branching, characterized in that: include: The pesticide preparation according to claim 1 or 2 is applied after alfalfa is mowed cleanly when it grows to the seedling stage after sowing.

4. The method according to claim 3, characterized in that The pesticide preparation includes benzylaminopurine 2.5×10 5 ~10 6 times solution, sodium nitrophenolate 10 7 times liquid, brassinolide 2.5×10 5 ~10 6 times solution, triacontanol 3.3×10 5 ~10 6 times liquid, uniconazole 2.5×10 5 ~10 6 times liquid.

5. The method according to claim 3 or 4, characterized in that: The application amount of the pesticide preparation is 100 mL / plant.

6. The method according to claim 3 or 4, characterized in that: The application site of the pesticide preparation is the above-ground part of alfalfa seedlings.

7. The method according to claim 3, characterized in that Alfalfa should be mowed flush with the ground at the seedling stage 20 days after sowing.

8. The method according to claim 3, characterized in that The branching position is the cutting cut.

9. The method according to claim 3, characterized in that: The alfalfa is harvested three times, the first harvest is carried out 50 days after sowing, the second harvest is carried out 35 days after the first harvest, and the third harvest is carried out 35 days after the second harvest.

10. The method according to claim 9, characterized in that The stubble height of the three mowings is 5 cm.

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