Lactobacillus fermentum strain culture device and culture method

Abstract

The invention relates to the field of strain culture, in particular to a fermentative milk strain culture device and culture method. Comprising a non-contact punching mechanism which is fixedly connected with the output end of the three-axis moving mechanism and comprises an air storage cylinder and an air gathering taper pipe, the air storage cylinder is arranged in a vertical state, and the air gathering taper pipe is detachably connected with the lower end of the air storage cylinder; the protection mechanism is connected with the air storage cylinder and comprises a partition bottom plate, a touch pressure sensor, a touch pressure rod, two sets of sterile films and two positioning arc covers, the two positioning arc covers are symmetrically arranged below the air storage cylinder, the partition bottom plate is fixedly connected with the lower ends of the two positioning arc covers, the touch pressure rod is arranged above the partition bottom plate, the touch pressure sensor is arranged above the touch pressure rod, and the sterile films are arranged on the two sets of sterile films. And the two groups of sterile films are adjustably arranged on the inner sides of the two positioning arc covers respectively.

Description

Technical Field

[0001] The invention relates to the field of bacterial strain cultivation, and in particular to a fermented lactobacillus bacterial strain cultivation device and a cultivation method. Background Art

[0002] When culturing the fermented lactic acid bacteria, the bacteria need to be mixed with the cooked materials and fermented. During the fermentation, a lot of hyphae will grow on the surface of the materials and gradually form agglomerated materials. Finally, the agglomerated materials are broken up and the broken up agglomerated materials can be further utilized. At the same time, the hyphae are kneaded off and collected to obtain the bacteria.

[0003] However, when the existing fermented lactic acid bacteria culture device is in use, the hyphae growing between the materials are the base hyphae, and the hyphae on the surface of the material will grow toward the upper space to form aerial hyphae, and such hyphae will gradually wither due to insufficient moisture and nutrients in the air, thereby affecting the harvest rate and quality of the culture. Although the current culture device uses holes in the material to allow external air to enter the agglomerated material, so as to guide the aerial hyphae on the surface of the material downward, to ensure that the hyphae can grow toward the inside of the material to form base hyphae, and to ensure the harvest rate and quality of the culture, in this process, the existing punching device mostly uses a mechanical structure to directly contact the material, so during the punching process, the mechanical structure will cross-mix the materials in different areas. If mold or other bacteria appear on the surface of the material in some areas at this time, all fermentation strains will be infected, thereby affecting the survival rate of the strains.

[0004] At the same time, because the mechanical structure squeezes the material when punching holes in the material and then inserts it into the agglomerated material again, it can guide all the aerial hyphae on the soybean surface. When the mechanical structure punches holes in the material, it will exert a certain squeezing force on the material. Since the texture and tightness of each part of the material may be different, the uneven squeezing force will cause the material to deform. This deformation may affect the air permeability and water retention of the material, and then affect the growth environment of the strain. In addition, excessive squeezing may also cause the nutritional structure inside the material to be destroyed, which is not conducive to the absorption and utilization of the strain. Summary of the invention

[0005] Based on this, it is necessary to provide a fermented lactic acid bacteria culture device and a culture method to address the existing technical problems.

[0006] In order to solve the problems of the prior art, the technical solution adopted by the present invention is: The fermented lactobacillus strain culture device comprises a culture box capable of placing a plurality of culture dishes and a three-axis moving mechanism arranged on the top of the culture box, and also comprises: The non-contact punching mechanism is fixedly connected to the output end of the three-axis moving mechanism, and includes an air storage cylinder and an air gathering cone tube. The air storage cylinder is arranged in a vertical state, and the air gathering cone tube is detachably connected to the lower end of the air storage cylinder; The protective mechanism is connected to the air storage cylinder and includes a partition bottom plate, a touch pressure sensor, a touch pressure rod, two groups of sterile membranes and two positioning arc covers. The two positioning arc covers are symmetrically arranged below the air storage cylinder, the partition bottom plate is fixedly connected to the lower ends of the two positioning arc covers, the touch pressure rod is arranged above the partition bottom plate, the touch pressure sensor is arranged above the touch pressure rod, and the two groups of sterile membranes are adjustably arranged on the inner sides of the two positioning arc covers.

[0007] Furthermore, the contactless punching mechanism also includes an active motor, a positioning plate and a positioning seat. The positioning seat is fixedly connected to the output end of the three-axis moving mechanism, the positioning plate is fixedly connected to the lower end of the positioning seat, the active motor is fixedly connected to one side of the positioning plate, a limited circular groove is formed on the side of the positioning plate away from the active motor, and a limited groove is formed on the side of the positioning seat away from the active motor.

[0008] Furthermore, the contactless punching mechanism also includes a reciprocating slider, a driving support rod, a pulling rocker and a limit pin. The reciprocating slider is slidably connected to the limit groove, one end of the pulling rocker is coaxially fixedly connected to the output end of the active motor, the limit pin is coaxially rotatably connected to the other end of the pulling rocker, one end of the limit pin is slidably connected to the limit circular groove, and the other end is rotatably connected to the driving support rod.

[0009] Furthermore, the contactless punching mechanism also includes a lifting base, a lifting roller, a positioning base, a piston, a positioning rod, a return spring and a trigger rod. The lifting base is fixedly connected to the lower end of the driving support rod, the lifting roller is rotatably connected to the lifting base, one end of the positioning base is fixedly connected to the air cylinder, and the other end is fixedly connected to the positioning plate, the positioning rod is coaxially slidably connected to the air cylinder, the piston is fixedly connected to the lower end of the positioning rod, the return spring is sleeved on the outside of the positioning rod, the upper end of the return spring is fixedly connected to the air cylinder, and the lower end is fixedly connected to the piston, one end of the trigger rod is fixedly connected to the upper end of the positioning rod, and the other end can be against the lifting roller during the rotation of the pulling rocker.

[0010] Furthermore, the contactless punching mechanism also includes a first one-way valve and a second one-way valve, the first one-way valve is fixedly connected to the side wall of the gas storage cylinder and communicated with the gas storage cylinder, and the second one-way valve is fixedly connected to the lower end of the gas gathering cone tube and communicated with the gas gathering cone tube.

[0011] Furthermore, the protection mechanism also includes a positioning top cover, a push spring, a positioning frame and a transfer bracket. The positioning top cover is coaxially sleeved on the outside of the gas storage cylinder and the lower end is slidably connected to the two positioning arc covers. The positioning frame is fixedly connected to the positioning top cover coaxially. The touch pressure sensor is fixedly connected to the lower end of one side of the positioning frame. The transfer bracket is fixedly connected to the upper ends of the two positioning arc covers. The push spring is sleeved on the outside of the positioning top cover. The upper end of the push spring is fixedly connected to the positioning top cover, and the lower end is fixedly connected to the upper end of the transfer bracket. The touch pressure rod is fixedly connected to the upper end of the transfer bracket.

[0012] Furthermore, the protection mechanism also includes a touch and pressure rack, a touch and pressure gear, an active bevel gear, a driven bevel gear and two driven gears. The touch and pressure rack is fixedly connected to the side of the positioning frame away from the touch and pressure sensor. The two driven gears are respectively rotatably connected to the side of the transfer bracket away from the touch and pressure rod. The driven bevel gear is coaxially fixed to a driven gear. The active bevel gear is meshed with the driven bevel gear. The touch and pressure gear is coaxially connected to the active bevel gear through a ratchet, and the touch and pressure rack can mesh with the touch and pressure gear.

[0013] Furthermore, the protection mechanism also includes two unwinding shafts and two winding shafts, the two winding shafts are respectively fixedly connected to the two driven gears coaxially, the upper ends of the two winding shafts are rotatably connected to the transfer bracket, and the lower ends are rotatably connected to the partition bottom plate, the two unwinding shafts are arranged on the side of the transfer bracket away from the two winding shafts, the upper ends of the two unwinding shafts are rotatably connected to the transfer bracket, and the lower ends are rotatably connected to the partition bottom plate, one end of the two groups of sterile films are respectively wrapped around the outside of the two unwinding shafts, and the other ends are respectively wrapped around the outside of the two winding shafts.

[0014] Furthermore, the protection mechanism also includes two protection plates, two positioning arc plates and a number of positioning rollers. The two positioning arc plates are respectively fixedly connected to the inner sides of the two positioning arc covers, and the number of positioning rollers are respectively rotatably connected to the sides close to the two positioning arc covers. The sterile film is slidably connected to the number of positioning rollers in turn, one end of the sterile film is slidably connected to the positioning arc plate, and the other end is slidably connected to the partition bottom plate. The two protection plates are respectively mounted on the outside of the winding shaft and the unwinding shaft, the upper end of the protection plate is fixedly connected to the transfer bracket, and the lower end is fixedly connected to the partition bottom plate.

[0015] The culture method of the fermented lactic acid bacteria culture device comprises the following culture steps: S1: When the device is in operation, the operator places several culture dishes carrying bacterial colonies on the incubator; S2: When it is necessary to punch holes on the bacterial colonies in the culture dish, the three-axis moving mechanism starts and drives the non-contact punching mechanism and the protection mechanism to move above the corresponding culture dish; S3: The non-contact punching mechanism then drives the gas storage cylinder to draw gas and eject the gas downward through the gas collecting cone tube, which collects the gas and punches holes on the bacterial colonies in the culture dish; S4: Punching can guide the aerial hyphae on the surface of the colony of the culture dish downward, ensuring that the hyphae can grow into the inside of the material to form intrabasic hyphae, thereby ensuring the harvest rate and quality of the culture.

[0016] Compared with the prior art, the present invention has the following beneficial effects: Firstly, the device realizes contactless punching of the culture dish through the gas storage cylinder and the gas collecting cone. During this process, the gas storage cylinder punches the upper end of the culture dish by sucking air, avoiding direct contact with the culture dish, improving the hygiene level during the punching process of the culture dish, and improving the quality of the culture dish; Second, the device isolates the culture dish during the punching process by separating the bottom plate, preventing the hyphae on the culture dish from flying around and affecting other culture dishes during the punching process, ensuring that each culture dish can maintain non-interference during the processing process, and reducing the probability of mixing between them; Thirdly, the device protects the culture dish through the sterile film to prevent the bacteria on the previous culture dish from falling onto the next culture dish when the next culture dish is punched, thereby improving the quality of the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a partial half-section diagram of the three-dimensional structure of the embodiment; Figure 2 It is a partial half-section diagram of the planar structure of the embodiment; Figure 3 Schematic diagram of the three-dimensional structure of the contactless punching mechanism and the protection mechanism in the embodiment; Figure 4 yes Figure 3 A magnified view of the structure at center; Figure 5 yes Figure 3 A magnified view of the structure at B in the middle; Figure 6 is a schematic diagram of the three-dimensional structure of the contactless punching mechanism and the protection mechanism in another angle in the embodiment; Figure 7 yes Figure 6 Schematic diagram of structural decomposition at C in the middle; Figure 8 is a half-section view of the three-dimensional structure of the protection mechanism in the embodiment; Fig. 9 It is a bottom view of the local structure of the protection mechanism in the embodiment.

[0018] The numbers in the figure are: 1. Incubator; 2. Culture dish; 3. Three-axis moving mechanism; 4. Contactless punching mechanism; 5. Active motor; 6. Positioning plate; 7. Limiting circular groove; 8. Positioning seat; 9. Limiting groove; 10. Reciprocating slider; 11. Driving support rod; 12. Pulling rocker; 13. Limiting pin; 14. Lifting base; 15. Lifting roller; 16. Positioning base; 17. Piston; 18. Positioning pull rod; 19. Reset spring; 20. Trigger pull rod; 21. Gas storage cylinder; 22. Gas gathering cone; 23. First unidirectional valve; 24, second one-way valve; 25, protective mechanism; 26, positioning top cover; 27, push spring; 28, positioning frame; 29, positioning arc cover; 30, transfer bracket; 31, partition bottom plate; 32, positioning arc plate; 33, positioning roller; 34, touch pressure sensor; 35, touch pressure rod; 36, unwinding shaft; 37, rewinding shaft; 38, protective plate; 39, sterile membrane; 40, touch pressure rack; 41, touch pressure gear; 42, ratchet; 43, active bevel gear; 44, driven bevel gear; 45, driven gear. DETAILED DESCRIPTION

[0019] In order to further understand the features, technical means, specific objectives and functions of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0020] refer to Figures 1 to 9 The fermented lactic acid bacteria culture device comprises a culture box 1 capable of placing a plurality of culture dishes 2 and a three-axis moving mechanism 3 arranged on the top of the culture box 1, and further comprises: The non-contact punching mechanism 4 is fixedly connected to the output end of the three-axis moving mechanism 3, and includes a gas storage cylinder 21 and a gas collecting cone 22 (refer to Figure 8 ), the gas storage cylinder 21 is arranged in a vertical state, and the gas collecting cone 22 is detachably connected to the lower end of the gas storage cylinder 21; The protection mechanism 25 is connected to the air storage cylinder 21, and includes a partition bottom plate 31, a touch pressure sensor 34, a touch pressure rod 35, two groups of sterile membranes 39 and two positioning arc covers 29. The two positioning arc covers 29 are symmetrically arranged below the air storage cylinder 21. The partition bottom plate 31 is fixedly connected to the lower ends of the two positioning arc covers 29, the touch pressure rod 35 is arranged above the partition bottom plate 31, the touch pressure sensor 34 is arranged above the touch pressure rod 35, and the two groups of sterile membranes 39 are respectively and arbitrarily arranged on the inner sides of the two positioning arc covers 29.

[0021] When the device is in operation, the culture dish 2 is placed on the incubator 1. When it is necessary to punch holes on the bacterial colonies of the culture dish 2, the three-axis moving mechanism 3 is started and drives the contactless punching mechanism 4 and the protective mechanism 25 to move above the corresponding culture dish 2. Then the three-axis moving mechanism 3 drives the contactless mechanism and the protective mechanism 25 to move downward until the partition bottom plate 31 contacts the bottom of the incubator 1. When the gas storage cylinder 21 moves toward the direction of the culture dish 2 until the touch pressure rod 35 contacts the touch pressure sensor 34, the touch pressure sensor 34 transmits this signal to the controller, and the controller controls the operation of the contactless punching mechanism 4. The contactless punching mechanism 4 will drive the gas storage cylinder 21 to exhaust gas and spray the gas downward through the gas gathering cone 22. The gas gathering cone 22 will gather the gas and punch holes on the bacterial colonies of the culture dish 2, so that the aerial hyphae on the upper surface of the culture dish 2 can be guided downward, ensuring that the hyphae can grow into the material to form intrabasic hyphae, thereby ensuring the harvest rate and quality of the culture of the strains.

[0022] During this process, the operator can change the size of the gas collecting cone 22 according to the type of culture medium on the culture dish 2, and then adjust the size of the aperture. At the same time, in order to prevent adjacent culture dishes 2 from affecting each other during the drilling process, the two positioning arc covers 29 will isolate the culture dishes 2 being drilled, and after the drilling is completed, the sterile membrane 39 set on the inner side of the positioning arc cover 29 will be replaced when the three-axis moving mechanism 3 is lifted upward to prevent individual bacteria species blown away by the air from adhering to the sterile membrane 39, and then when the next culture dish 2 is isolated, these bacteria species will affect the colonies on the culture dish 2.

[0023] In order to supplement the structure of the contactless punching mechanism 4, the following features are also specifically provided: The contactless punching mechanism 4 also includes an active motor 5, a positioning plate 6 and a positioning seat 8, and the positioning seat 8 is fixedly connected to the output end of the three-axis moving mechanism 3 (refer to Figure 3 ), the positioning plate 6 is fixedly connected to the lower end of the positioning seat 8, the active motor 5 is fixedly connected to one side of the positioning plate 6, a limited circular groove 7 is formed on the side of the positioning plate 6 away from the active motor 5, and a limited groove 9 is formed on the side of the positioning seat 8 away from the active motor 5. After the three-axis moving mechanism 3 is started, the three-axis moving mechanism 3 will drive the positioning plate 6 to move through the positioning seat 8 until it moves to the top of the corresponding culture dish 2.

[0024] In order to further supplement the structure of the contactless punching mechanism 4, the following features are also specifically provided: The non-contact punching mechanism 4 further includes a reciprocating slider 10, a driving support rod 11, a pulling rocker 12 and a limiting pin 13. The reciprocating slider 10 is slidably connected to the limiting groove 9 (refer to Figure 3), one end of the pulling rocker 12 is coaxially fixedly connected to the output end of the active motor 5, the limit pin 13 is coaxially rotatably connected to the other end of the pulling rocker 12, and one end of the limit pin 13 is slidably connected to the limit circular groove 7 (reference Figure 4 ), and the other end is rotatably connected to the driving support rod 11. After the active motor 5 is started, the active motor 5 will drive the limit pin 13 to move along the limit circular groove 7 by pulling the rocker 12. During this process, the limit pin 13 will drive the driving support rod 11 to move, and when the driving support rod 11 moves, it will be limited by the reciprocating slider 10 (refer to Figure 3 ), that is, at this time, the lower end of the driving support rod 11 will be displaced in the vertical direction, and the displacement trajectory is circular.

[0025] In order to realize that when the active motor 5 is started, the air storage cylinder 21 will spray downward through the air collecting cone 22, the following features are also specifically provided: The non-contact punching mechanism 4 also includes a lifting base 14, a lifting roller 15, a positioning base 16, a piston 17, a positioning rod 18, a return spring 19 and a trigger rod 20. The lifting base 14 is fixedly connected to the lower end of the driving support rod 11 (refer to Figure 5 ), the lifting roller 15 is rotatably connected to the lifting base 14, and one end of the positioning base 16 is fixedly connected to the air storage cylinder 21 (such as Figure 3 As shown, the positioning base 16 is in a "9" shape as a whole), the other end is fixedly connected to the positioning plate 6, the positioning rod 18 is coaxially slidably connected to the gas storage cylinder 21, and the piston 17 is fixedly connected to the lower end of the positioning rod 18 (reference Figure 8 ), the return spring 19 is sleeved on the outside of the positioning rod 18, the upper end of the return spring 19 is fixedly connected to the air storage cylinder 21, and the lower end is fixedly connected to the piston 17, one end of the trigger rod 20 is fixedly connected to the upper end of the positioning rod 18, and the other end can be against the pulling roller 15 during the rotation of the pulling rocker 12. When the driving support rod 11 moves, the driving support rod 11 will drive the lifting roller 15 to move through the lifting base 14. When the lifting roller 15 moves to contact with the trigger rod 20, the lifting roller 15 moves upward and moves upward through the trigger rod 20. At this time, the reset spring 19 will be compressed, and the corresponding air storage cylinder 21 will inhale air. However, because the moving trajectory of the lifting roller 15 is circular, the lifting roller 15 will eventually separate from the trigger rod 20. Then the reset spring 19 will push the piston 17 to move downward, and the air in the air storage cylinder 21 will be ejected downward through the gas collection cone 22. The ejected air will perform contactless punching on the colonies in the culture dish 2 at the upper end of the culture dish 2. In this process, because the reset spring 19 can also provide elastic buffering during the deformation process, this reduces the vibration of the air storage cylinder 21 when it ejects air downward, thereby ensuring that the aperture will not be offset when punching the colonies, and the colonies will not come into contact with the device during the vibration of the device.

[0026] In order to facilitate the air to be sucked into the air storage cylinder 21, the following features are also specifically provided: The non-contact punching mechanism 4 further includes a first one-way valve 23 and a second one-way valve 24. The first one-way valve 23 is fixedly connected to the side wall of the gas storage cylinder 21 and communicates with the gas storage cylinder 21 (refer to Figure 8 ), the second one-way valve 24 is fixedly connected to the lower end of the gas collecting cone 22 and communicates with the gas collecting cone 22. When the piston 17 moves upward, air will flow into the gas storage cylinder 21 from the first one-way valve 23, and when the piston 17 moves downward, air will be ejected from the gas collecting cone 22 from the second one-way valve 24, ensuring that the air can flow in the gas storage cylinder 21 in one direction.

[0027] In order to supplement the specific structure of the protection mechanism 25, the following features are also specifically provided: The protection mechanism 25 also includes a positioning top cover 26, a push spring 27, a positioning frame 28 and a transfer bracket 30. The positioning top cover 26 is coaxially sleeved on the outside of the gas storage cylinder 21 and the lower end is slidably connected with two positioning arc covers 29 (refer to Figure 8 ), the positioning frame 28 is coaxially connected to the positioning top cover 26, the touch pressure sensor 34 is connected to the lower end of one side of the positioning frame 28, the transfer bracket 30 is connected to the upper ends of the two positioning arc covers 29, the push spring 27 is sleeved on the outside of the positioning top cover 26, the upper end of the push spring 27 is connected to the positioning top cover 26, the lower end is connected to the upper end of the transfer bracket 30, and the touch pressure rod 35 is connected to the upper end of the transfer bracket 30. After the three-axis moving mechanism 3 moves to the top of the culture dish 2, as the three-axis moving mechanism 3 drives the partition bottom plate 31 to press against the side of the culture dish 2, the output end of the three-axis moving mechanism 3 continues to move downward, and the push spring 27 will be compressed. At this time, the positioning top cover 26 will be close to the two positioning arc covers 29 until the touch pressure rod 35 contacts the touch pressure sensor 34, and then the touch pressure sensor 34 transmits this signal to the controller, and the controller controls the active motor 5 to start.

[0028] In order to further supplement the specific structure of the protection mechanism 25, the following features are also specifically provided: The protection mechanism 25 further includes a contact pressure rack 40, a contact pressure gear 41, an active bevel gear 43, a driven bevel gear 44 and two driven gears 45. The contact pressure rack 40 is fixedly connected to a side of the positioning frame 28 away from the contact pressure sensor 34 (refer to Figure 8 ), the two driven gears 45 are respectively connected to the side of the transfer bracket 30 away from the touch pressure rod 35, and the driven bevel gear 44 is fixedly connected to the driven gear 45 coaxially (refer to Figure 7), the active bevel gear 43 meshes with the driven bevel gear 44, the contact pressure gear 41 is coaxially connected to the active bevel gear 43 through the ratchet 42, and the contact pressure rack 40 can mesh with the contact pressure gear 41. When the positioning top cover 26 and the two positioning arc covers 29 are close to each other, the contact pressure rack 40 will mesh with the contact pressure gear 41, but under the action of the ratchet 42, the rotation of the contact pressure gear 41 at this time will not drive the active bevel gear 43 to rotate, and when the drilling is completed and the positioning top cover 26 and the two positioning arc covers 29 are separated, the contact pressure rack 40 will mesh with the contact pressure gear 41 again. At this time, under the action of the ratchet 42, the contact pressure gear 41 will drive the driven bevel gear 44 to rotate through the active bevel gear 43, and the driven bevel gear 44 will drive the two driven gears 45 to rotate in sequence after rotating (refer to Figure 7 ).

[0029] In order to facilitate the unwinding and rewinding of the sterile film 39, the following features are also specifically provided: The protection mechanism 25 further includes two unwinding shafts 36 and two rewinding shafts 37. The two rewinding shafts 37 are respectively coaxially connected to the two driven gears 45 (refer to Figure 7 ), the upper ends of the two reeling shafts 37 are rotatably connected to the transfer bracket 30, and the lower ends are rotatably connected to the partition bottom plate 31. The two unwinding shafts 36 are arranged on the side of the transfer bracket 30 away from the two rewinding shafts 37. The upper ends of the two unwinding shafts 36 are rotatably connected to the transfer bracket 30, and the lower ends are rotatably connected to the partition bottom plate 31. One end of the two groups of sterile membranes 39 are respectively wound around the outside of the two unwinding shafts 36, and the other ends are respectively wound around the outside of the two rewinding shafts 37. When the two driven gears 45 rotate, the two driven gears 45 will drive the two reeling shafts 37 fixed thereto to rotate, and the two reeling shafts 37 will drive the sterile membrane 39 to move after rotating, so as to ensure that the sterile membrane 39 can be kept clean when the colonies on the culture dish 2 are punched.

[0030] In order to prevent the sterile membrane 39 from being able to move normally, the following features are also specifically provided: The protection mechanism 25 also includes two protection plates 38, two positioning arc plates 32 and a plurality of positioning rollers 33. The two positioning arc plates 32 are respectively fixedly connected to the inner sides of the two positioning arc covers 29 (refer to Figure 8 ), a plurality of positioning rollers 33 are rotatably connected to the sides of the two positioning arc covers 29 (reference Fig. 9), the sterile film 39 is slidably connected to a plurality of positioning rollers 33 in sequence, one end of the sterile film 39 is slidably connected to the positioning arc plate 32, and the other end is slidably connected to the partition bottom plate 31, and two protective plates 38 are respectively sleeved on the outside of the winding shaft 37 and the unwinding shaft 36, the upper end of the protective plate 38 is fixedly connected to the transfer bracket 30, and the lower end is fixedly connected to the partition bottom plate 31. When the sterile membrane 39 moves, the sterile membrane 39 will reduce the friction during the movement through the shafts of the positioning rollers 33, and the positioning arc plate 32 and the partition bottom plate 31 can limit the sterile membrane 39 (at the same time, the middle part of the partition bottom plate 31 is rotatably provided with a fine roller to guide the sterile membrane 39. When the sterile membrane 39 is not in contact with the culture dish 2, the sterile membrane 39 will remain in a tight state under the action of the fine roller, and when the sterile membrane 39 is in contact with the culture dish 2, the culture dish 2 will open the sterile membrane 39 to ensure that the sterile membrane 39 can be wrapped around the culture dish 2), ensuring that the sterile membrane 39 is tightly attached to the shafts of the positioning rollers 33. The protective plate 38 can ensure that the sterile membrane 39 will not be contaminated by the bacteria floating in the air in the incubator 1.

[0031] The culture method of the fermented lactic acid bacteria culture device comprises the following culture steps: S1: When the device is in operation, the operator places a number of culture dishes 2 carrying bacterial colonies on the incubator 1; S2: When it is necessary to punch a hole on the bacterial colony of the culture dish 2, the three-axis moving mechanism 3 is started and drives the non-contact punching mechanism 4 and the protection mechanism 25 to move above the corresponding culture dish 2; S3: Then the non-contact punching mechanism 4 drives the gas storage cylinder 21 to draw gas and spray the gas downward through the gas collecting cone 22. The gas collecting cone 22 collects the gas and punches holes on the bacterial colonies of the culture dish 2. S4: Punching can guide the aerial hyphae on the upper surface of the culture dish 2 downward, so as to ensure that the hyphae can grow into the inside of the material to form intrabasic hyphae, thereby ensuring the harvest rate and quality of the culture of the strains.

[0032] The working principle of the device is that when the device is in operation, the culture dish 2 is placed on the incubator 1. When it is necessary to punch holes on the colonies of the culture dish 2, the three-axis moving mechanism 3 will drive the positioning plate 6 to move through the positioning seat 8 until the gas storage cylinder 21 moves to the top of the corresponding culture dish 2, and then the three-axis moving mechanism 3 drives the device as a whole to move downward until the partition bottom plate 31 and the bottom of the incubator 1 are in contact. When the gas storage cylinder 21 moves toward the direction of the culture dish 2 until the touch pressure rod 35 contacts the touch pressure sensor 34, the touch pressure sensor 34 transmits this signal to the controller, and the controller controls the active motor 5 to operate. Then the gas storage cylinder 21 will draw air and spray the gas downward through the gas gathering cone 22. The gas gathering cone 22 will gather the gas and punch holes on the colonies of the culture dish 2, so that the aerial hyphae on the surface of the colonies of the culture dish 2 can be guided downward, ensuring that the hyphae can grow into the material to form intrabasic hyphae, thereby ensuring the harvest rate and quality of the culture of the strains.

[0033] During this process, the operator can change the size of the gas collecting cone 22 according to the type of culture medium on the culture dish 2, and then adjust the size of the aperture. At the same time, in order to prevent adjacent culture dishes 2 from affecting each other during the drilling process, the two positioning arc covers 29 will isolate the culture dishes 2 being drilled, and after the drilling is completed, the sterile membrane 39 set on the inner side of the positioning arc cover 29 will be replaced when the three-axis moving mechanism 3 is lifted upward to prevent individual bacteria species blown away by the air from adhering to the sterile membrane 39, and then when the next culture dish 2 is isolated, these bacteria species will affect the colonies in the culture dish 2.

[0034] The above embodiments only express one or several implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present invention. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the attached claims.

Claims

1. A fermented lactic acid bacteria culture device, comprising a culture box (1) capable of accommodating a plurality of culture dishes (2) and a three-axis moving mechanism (3) arranged on the top of the culture box (1), characterized in that: Also includes: The non-contact punching mechanism (4) is fixedly connected to the output end of the three-axis moving mechanism (3), and comprises an air storage cylinder (21) and an air gathering cone tube (22), wherein the air storage cylinder (21) is arranged in a vertical state, and the air gathering cone tube (22) is detachably connected to the lower end of the air storage cylinder (21); The protection mechanism (25) is connected to the gas storage cylinder (21), and comprises a partition bottom plate (31), a touch pressure sensor (34), a touch pressure rod (35), two groups of sterile membranes (39) and two positioning arc covers (29). The two positioning arc covers (29) are symmetrically arranged below the gas storage cylinder (21), the partition bottom plate (31) is fixedly connected to the lower ends of the two positioning arc covers (29), the touch pressure rod (35) is arranged above the partition bottom plate (31), the touch pressure sensor (34) is arranged above the touch pressure rod (35), and the two groups of sterile membranes (39) are respectively and arbitrarily arranged on the inner sides of the two positioning arc covers (29).

2. The fermented lactic acid bacteria culture device according to claim 1, characterized in that: The contactless punching mechanism (4) further comprises an active motor (5), a positioning plate (6) and a positioning seat (8); the positioning seat (8) is fixedly connected to the output end of the three-axis moving mechanism (3); the positioning plate (6) is fixedly connected to the lower end of the positioning seat (8); the active motor (5) is fixedly connected to one side of the positioning plate (6); a limited circular groove (7) is formed on the side of the positioning plate (6) away from the active motor (5); and a limited groove (9) is formed on the side of the positioning seat (8) away from the active motor (5).

3. The fermented lactic acid bacteria culture device according to claim 2, characterized in that: The contactless punching mechanism (4) further comprises a reciprocating slider (10), a driving support rod (11), a pulling rocker (12) and a limit pin (13); the reciprocating slider (10) is slidably connected to the limit groove (9); one end of the pulling rocker (12) is coaxially fixedly connected to the output end of the active motor (5); the limit pin (13) is coaxially rotatably connected to the other end of the pulling rocker (12); one end of the limit pin (13) is slidably connected to the limit circular groove (7); and the other end is rotatably connected to the driving support rod (11).

4. The fermented lactic acid bacteria culture device according to claim 3, characterized in that: The non-contact punching mechanism (4) further comprises a lifting base (14), a lifting roller (15), a positioning base (16), a piston (17), a positioning rod (18), a return spring (19) and a trigger rod (20); the lifting base (14) is fixedly connected to the lower end of the driving support rod (11); the lifting roller (15) is rotatably connected to the lifting base (14); one end of the positioning base (16) is fixedly connected to the air storage cylinder (21) and the other end is fixedly connected to the positioning plate (6); The pull rod (18) is slidably connected to the gas storage cylinder (21) coaxially, the piston (17) is fixedly connected to the lower end of the positioning pull rod (18), the return spring (19) is sleeved on the outside of the positioning pull rod (18), the upper end of the return spring (19) is fixedly connected to the gas storage cylinder (21), and the lower end is fixedly connected to the piston (17), one end of the trigger pull rod (20) is fixedly connected to the upper end of the positioning pull rod (18), and the other end can be abutted against the lifting roller (15) during the rotation of the pulling rocker (12).

5. The fermented lactic acid bacteria culture device according to claim 4, characterized in that: The non-contact punching mechanism (4) further comprises a first one-way valve (23) and a second one-way valve (24); the first one-way valve (23) is fixedly connected to the side wall of the gas storage cylinder (21) and is in communication with the gas storage cylinder (21); and the second one-way valve (24) is fixedly connected to the lower end of the gas gathering cone tube (22) and is in communication with the gas gathering cone tube (22).

6. The fermented lactic acid bacteria culture device according to claim 5, characterized in that: The protection mechanism (25) further comprises a positioning top cover (26), a push spring (27), a positioning frame (28) and a transfer bracket (30); the positioning top cover (26) is coaxially sleeved on the outside of the gas storage cylinder (21) and the lower end is slidably connected to the two positioning arc covers (29); the positioning frame (28) is coaxially fixedly connected to the positioning top cover (26); the touch pressure sensor (34) is fixedly connected to the lower end of one side of the positioning frame (28); the transfer bracket (30) is fixedly connected to the upper ends of the two positioning arc covers (29); the push spring (27) is sleeved on the outside of the positioning top cover (26); the upper end of the push spring (27) is fixedly connected to the positioning top cover (26); the lower end is fixedly connected to the upper end of the transfer bracket (30); and the touch pressure rod (35) is fixedly connected to the upper end of the transfer bracket (30).

7. The fermented lactic acid bacteria culture device according to claim 6, characterized in that: The protection mechanism (25) further comprises a touch pressure rack (40), a touch pressure gear (41), an active bevel gear (43), a driven bevel gear (44) and two driven gears (45); the touch pressure rack (40) is fixedly connected to a side of the positioning frame (28) away from the touch pressure sensor (34); the two driven gears (45) are respectively rotatably connected to a side of the transfer bracket (30) away from the touch pressure rod (35); the driven bevel gear (44) is coaxially fixedly connected to a driven gear (45); the active bevel gear (43) is meshed with the driven bevel gear (44); the touch pressure gear (41) is coaxially connected to the active bevel gear (43) through a ratchet (42); and the touch pressure rack (40) can mesh with the touch pressure gear (41).

8. The fermented lactic acid bacteria culture device according to claim 7, characterized in that: The protection mechanism (25) further comprises two unwinding shafts (36) and two reeling shafts (37), the two reeling shafts (37) being respectively coaxially fixedly connected to the two driven gears (45), the upper ends of the two reeling shafts (37) being rotatably connected to the transfer bracket (30), and the lower ends being rotatably connected to the partition bottom plate (31), the two unwinding shafts (36) being arranged on a side of the transfer bracket (30) away from the two reeling shafts (37), the upper ends of the two unwinding shafts (36) being rotatably connected to the transfer bracket (30), and the lower ends being rotatably connected to the partition bottom plate (31), and one end of the two groups of sterile membranes (39) being respectively wound around the outside of the two unwinding shafts (36), and the other ends being respectively wound around the outside of the two reeling shafts (37).

9. The fermented lactic acid bacteria culture device according to claim 8, characterized in that: The protection mechanism (25) further comprises two protection plates (38), two positioning arc plates (32) and a plurality of positioning rollers (33). The two positioning arc plates (32) are respectively fixedly connected to the inner sides of the two positioning arc covers (29). The plurality of positioning rollers (33) are respectively rotatably connected to the sides of the two positioning arc covers (29) that are close to each other. The sterile membrane (39) is slidably connected to the plurality of positioning rollers (33) in sequence. One end of the sterile membrane (39) is slidably connected to the positioning arc plates (32), and the other end is slidably connected to the partition bottom plate (31). The two protection plates (38) are respectively sleeved on the outside of the reeling shaft (37) and the unreeling shaft (36). The upper end of the protection plate (38) is fixedly connected to the transfer bracket (30), and the lower end is fixedly connected to the partition bottom plate (31).

10. A method for cultivating a fermented lactic acid bacteria culture device, comprising the fermented lactic acid bacteria culture device according to claim 1, characterized in that: The following cultivation steps are also included: S1: When the device is in operation, an operator places a plurality of culture dishes (2) carrying bacterial colonies on an incubator (1); S2: When it is necessary to punch a hole on the bacterial colony of the culture dish (2), the three-axis moving mechanism (3) is started and drives the non-contact punching mechanism (4) and the protection mechanism (25) to move to the top of the corresponding culture dish (2); S3: The non-contact punching mechanism (4) then drives the gas storage cylinder (21) to draw gas and eject the gas downward through the gas collecting cone (22). The gas collecting cone (22) collects the gas and punches holes on the bacterial colony of the culture dish (2). S4: Punching holes can guide the aerial hyphae on the surface of the colony of the culture dish (2) downward, so as to ensure that the hyphae can grow into the interior of the material to form intrabasic hyphae, thereby ensuring the harvest rate and quality of the culture of the strain.

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