Preparation method of mycelium composite material

Abstract

The invention relates to a preparation method of a mycelium composite material. The preparation method comprises the following steps: step 1, activating a strain; 2, preparing a culture medium, wherein the culture medium comprises a carbon source, a nitrogen source and mineral salt; step 3, inoculating the selected strain into a culture medium; 4, culturing under the conditions of proper temperature, relative humidity and ventilation to promote the growth of mycelia; and 5, separating and extracting the cultured mycelium. The invention provides a preparation method and application of a mycelium composite material. Microbial strains with excellent growth performance are selected, and an appropriate culture medium is prepared according to the growth requirements of the microbial strains. The strain is cultured under proper culture conditions, and high-quality mycelia are obtained. The obtained composite mycelium can be used for packaging food, medicine and other materials.

Description

Technical Field

[0001] The invention relates to the technical field of packaging materials, and in particular to a method for preparing a mycelium composite material. Background Art

[0002] As one of the five major packaging materials, plastic has been widely used in packaging due to its wide range of excellent physical and chemical properties such as light weight and high performance. It has replaced traditional packaging materials in many occasions and become the protagonist of packaging. Plastic packaging accounts for about 30% of the entire packaging, and packaging accounts for about 3% of the global annual total of nearly 100 million tons of plastic. As an important part of modern materials, it can be said that people cannot live without plastic for a day. Many products cannot be launched without plastic. However, plastic packaging belongs to the "short-life" application category of plastic. There are only two ways for plastic packaging after use: becoming plastic garbage thrown away at will, or becoming urban solid waste. Either way, it will harm the environment. In addition, due to the special environmental stability of plastic, it will not decompose or decompose very slowly in the environment for a long time, decades or even hundreds of years, so the harm to the environment is very long-lasting. With the increasing understanding of human beings on environmental pollution and ecological protection, the environmental pollution problem of packaging waste is also increasingly concerned.

[0003] Mycelium composite materials are widely used in the fields of construction, packaging, furniture, etc. due to their good plasticity and formability. However, under the existing technical conditions, the preparation method of mycelium composite materials has the following problems due to its own principle limitations: First, the mechanical strength of the product is low. Second, the preparation process is complicated and the preparation cost is high. Based on this, it is necessary to invent a new method for preparing mycelium composite materials to solve the problems of low mechanical strength, complicated preparation process and high preparation cost of the products prepared by the existing mycelium composite material preparation method. Summary of the invention

[0004] The purpose of the invention is to provide a method for preparing a mycelium composite material.

[0005] To achieve the above-mentioned and other related purposes, the present invention provides a technical solution: a method for preparing a mycelium composite material, comprising the following steps: Step 1: Activate the bacteria; Step 2: preparing a culture medium, which includes a carbon source, a nitrogen source, and mineral salts; Step 3: Inoculate the selected strain into the culture medium; Step 4: Cultivate under suitable temperature, relative humidity and ventilation conditions to promote the growth of mycelium; Step 5: Separate and extract the cultured mycelium.

[0006] The preferred technical solution is: the fungus species is Ganoderma lucidum or white rot fungus.

[0007] The preferred technical solution is: the carbon source includes glucose and lignocellulose; the nitrogen source includes yeast extract and protein hydrolyzate; and the mineral salt includes magnesium sulfate and potassium dihydrogen phosphate.

[0008] The preferred technical solution is: 45-55 parts by weight of corn straw, 35-45 parts by weight of bran, 5-10 parts by weight of soybean meal, 1-2 parts by weight of glucose, 2-5 parts by weight of casein, 1-2 parts by weight of magnesium sulfate, and 1-2 parts by weight of potassium dihydrogen phosphate; 50.5±0.5 parts by weight of water, and a pH value of 8.5±0.5.

[0009] The preferred technical solution is: in step 4, the temperature is 20-38°C, the relative humidity is 60-80%, and the ventilation ratio is 0.3-0.6V / V·min.

[0010] The preferred technical solution is: separation and extraction include: the method of mycelium includes centrifugation and filtration.

[0011] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art: The present invention provides a preparation method and application of a mycelium composite material. The present invention selects a microbial strain with excellent growth performance and configures a suitable culture medium according to its growth requirements. By culturing the strain under suitable culture conditions, high-quality mycelium is obtained. The obtained composite mycelium can be used for packaging of food, medicine and other materials. DETAILED DESCRIPTION

[0012] The following specific embodiments illustrate the implementation of the present invention, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in the embodiments. Unless otherwise specified, the experimental materials used in the following embodiments were purchased from conventional biochemical reagent stores.

[0013] Example 1: A method for preparing a mycelium composite material A method for preparing a mycelium composite material comprises the following steps: Step 1: Activate the bacteria; Step 2: preparing a culture medium, which includes a carbon source, a nitrogen source, and mineral salts; Step 3: Inoculate the selected strain into the culture medium; Step 4: Cultivate under suitable temperature, relative humidity and ventilation conditions to promote the growth of mycelium; Step 5: Separate and extract the cultured mycelium.

[0014] A preferred embodiment is that the fungus species is Ganoderma lucidum or white rot fungus.

[0015] A preferred embodiment is as follows: the carbon source includes glucose and lignocellulose; the nitrogen source includes yeast extract and protein hydrolyzate; and the mineral salt includes magnesium sulfate and potassium dihydrogen phosphate.

[0016] The preferred embodiment is: 45 parts by weight of corn straw, 35 parts by weight of bran, 5 parts by weight of soybean meal, 1 part by weight of glucose, 2 parts by weight of casein, 1 part by weight of magnesium sulfate, 1 part by weight of potassium dihydrogen phosphate; 50 parts by weight of water, and a pH value of 8.

[0017] A preferred implementation is: in step 4, the temperature is 20° C., the relative humidity is 60%, and the ventilation ratio is 0.3 V / V·min.

[0018] The preferred embodiment is that the separation and extraction of mycelium includes: the method of centrifugation and filtration.

[0019] When the mycelium grows to a certain extent, the mycelium can be separated from the culture medium by separation and extraction methods. Commonly used methods include centrifugation, dehydration, filtration, etc. The mycelium finally obtained can be used for subsequent production applications.

[0020] In actual operation, each step requires strict control of conditions to ensure the quality and yield of mycelium. In addition, different strains may have different requirements for culture conditions, so optimization and adjustment may be required for specific strains. In particular, the growth rate of the composite mycelium material needs to be controlled, which will affect its mechanical properties (including wet tensile strength, elongation at break, and tear strength).

[0021] Example 2: A method for preparing a mycelium composite material A method for preparing a mycelium composite material, characterized in that it comprises the following steps: Step 1: Activate the bacteria; Step 2: preparing a culture medium, which includes a carbon source, a nitrogen source, and mineral salts; Step 3: Inoculate the selected strain into the culture medium; Step 4: Cultivate under suitable temperature, relative humidity and ventilation conditions to promote the growth of mycelium; Step 5: Separate and extract the cultured mycelium.

[0022] A preferred embodiment is that the fungus species is white rot fungus.

[0023] A preferred embodiment is as follows: the carbon source includes glucose and lignocellulose; the nitrogen source includes yeast extract and protein hydrolyzate; and the mineral salt includes magnesium sulfate and potassium dihydrogen phosphate.

[0024] The preferred embodiment is: 55 parts by weight of corn straw, 5 parts by weight of bran, 10 parts by weight of soybean meal, 2 parts by weight of glucose, 5 parts by weight of casein, 2 parts by weight of magnesium sulfate, and 2 parts by weight of potassium dihydrogen phosphate; 50 parts by weight of water, and a pH value of 9.

[0025] A preferred implementation is: in step 4, the temperature is 38° C., the relative humidity is 80%, and the ventilation ratio is 0.6 V / V·min.

[0026] The preferred embodiment is that the separation and extraction of mycelium includes: the method of centrifugation and filtration.

[0027] The above description is only used to explain the preferred embodiments of the present invention, and is not intended to limit the present invention in any form. Therefore, any modifications or changes made to the present invention under the same inventive spirit should still be included in the scope of protection intended by the present invention.

Claims

1. A method for preparing a mycelium composite material, characterized in that: The following steps are involved: Step 1: Activate the bacteria; Step 2: preparing a culture medium, which includes a carbon source, a nitrogen source, and mineral salts; Step 3: Inoculate the selected strain into the culture medium; Step 4: Cultivate under suitable temperature, relative humidity and ventilation conditions to promote the growth of mycelium; Step 5: Separate and extract the cultured mycelium.

2. The method for preparing the mycelium composite material according to claim 1, characterized in that: The fungus species is ganoderma lucidum or white rot fungus.

3. The method for preparing the mycelium composite material according to claim 1, characterized in that: Carbon sources include glucose and lignocellulose; nitrogen sources include yeast extract and protein hydrolyzate; mineral salts include magnesium sulfate and potassium dihydrogen phosphate.

4. The method for preparing the mycelium composite material according to claim 3, characterized in that: The culture medium formula is: 45-55 parts by weight of corn straw, 35-45 parts by weight of bran, 5-10 parts by weight of soybean meal, 1-2 parts by weight of glucose, 2-5 parts by weight of casein, 1-2 parts by weight of magnesium sulfate, and 1-2 parts by weight of potassium dihydrogen phosphate; The amount of water is 50.5±0.5 parts by weight and the pH value is 8.5±0.

5.

5. The method for preparing the mycelium composite material according to claim 1, characterized in that: In step 4, the temperature is 20-38°C, the relative humidity is 60-80%, and the ventilation ratio is 0.3-0.6V / V·min.

6. The method for preparing the mycelium composite material according to claim 1, characterized in that: Separation and extraction methods include: mycelium centrifugation and filtration.