Column oven capable of controlling temperature in two directions

Abstract

The invention provides a two-way temperature control column oven which comprises a first cavity defined by an inner container, a refrigerating and heating device and a door body and a second cavity defined by the inner container, the refrigerating and heating device and an oven body, a first fan and a first cooling fin are arranged in the first cavity, and a second fan and a second cooling fin are arranged in the second cavity. A first fan and a first cooling fin are arranged in the first cavity, a second fan and a second cooling fin are arranged in the second cavity, and therefore when the refrigerating and heating device conducts heating on one side of the first cavity, a cold load generated on one side of the second cavity can be discharged out of the second cavity through the second fan and the second cooling fin to prevent the cold load from being transmitted into the first cavity, and then the temperature in the first cavity is kept stable. When the refrigerating and heating device refrigerates at one side of the first cavity, heat generated at one side of the second cavity can be discharged out of the second cavity through the second fan and the second cooling fins to prevent the heat from being transferred into the first cavity, so that the temperature in the first cavity is kept stable, and the separation accuracy of the chromatographic column is improved.

Description

Technical Field

[0001] The present application relates to the technical field of analytical instruments and equipment, and in particular to a column temperature box capable of bidirectional temperature control. Background Art

[0002] The temperature of the liquid chromatography column has a great influence on the adsorption analysis results of the components therein. The temperature change and the temperature difference in the column oven will affect the accuracy of the separation results of the chromatographic column.

[0003] In a traditional column oven, due to unstable air circulation in the oven, the heat or cold load in the oven will be transferred to the reaction chamber of the chromatographic column, resulting in unstable temperature in the reaction chamber of the chromatographic column, which in turn causes low accuracy of the chromatographic column separation results. Summary of the invention

[0004] In order to solve the above technical problems, the present application provides a column temperature oven capable of bidirectional temperature control, comprising a box body and a door body, wherein the box body and the door body enclose a cavity; An inner tank and a cooling and heating device are arranged in the cavity, the inner tank is fixedly arranged on the box body, and the cooling and heating device is fixedly arranged on a side of the inner tank away from the door body; The inner tank, the refrigeration and heating device and the door body enclose a closed first cavity; the inner tank, the box body and the refrigeration and heating device enclose a second cavity; the chromatographic column is arranged in the first cavity; A first fan and a first heat sink are disposed in the first cavity to drive air to move in the first cavity; a second fan and a second heat sink are disposed in the second cavity to drive air to move in the second cavity.

[0005] In some embodiments of the present application, the inner tank includes an air guide shell, a fixed chromatographic column shell and an adapter; the adapter is fixed on the casing, the fixed chromatographic column shell is fixedly connected to a side of the adapter away from the door body, and the air guide shell is fixedly connected to a side of the fixed chromatographic column shell away from the door body.

[0006] In some embodiments of the present application, the air guide housing includes an inclined air guide surface, which can guide the movement of air in the main cavity and accelerate the circulation of air in the main cavity.

[0007] In some embodiments of the present application, a first groove and a second groove that are interconnected are provided on one side of the adapter close to the door body, the first groove and the second groove together form a convex groove facing the side of the fixed chromatography column housing, and a plurality of mounting holes for realizing a threaded connection with the fixed chromatography column housing are spaced apart in the first groove.

[0008] In some embodiments of the present application, a sealing member is disposed in the convex groove.

[0009] In some embodiments of the present application, the exterior of the inner tank is covered with a heat preservation plate to prevent the heat or cold load in the second cavity from being transferred to the first cavity.

[0010] In some embodiments of the present application, an upper partition and a lower partition are provided in the second cavity to divide the second cavity into an upper cavity, a middle cavity and a lower cavity.

[0011] In some embodiments of the present application, a third fan and a fourth fan are arranged in an upper and lower spaced relationship in the middle cavity on a side away from the inner tank.

[0012] In some embodiments of the present application, a first temperature sensor and a second temperature sensor are disposed in the inner tank, the first temperature sensor is disposed on a side of the first fan, and the second temperature sensor is disposed in the first heat sink.

[0013] In some embodiments of the present application, the outside of the insulation board is also covered with a cover plate, and fasteners are threadedly connected to the upper partition and the lower partition, and the ends of the fasteners abut against the cover plate to apply a clamping force to the inner tank in the direction of the door body to further seal the first cavity.

[0014] Compared with the prior art, the present invention has the following advantages and beneficial effects: the column temperature box with bidirectional temperature control of the present application includes a first cavity formed by an inner tank, a cooling and heating device and a door body, and a second cavity formed by an inner tank, a cooling and heating device and a box body. The first cavity is provided with a first fan and a first heat sink, and the second cavity is provided with a second fan and a second heat sink. In this way, when the cooling and heating device heats one side of the first cavity, the cold load generated on one side of the second cavity can be discharged out of the second cavity through the second fan and the second heat sink to prevent the cold load from being transferred to the first cavity, thereby keeping the temperature in the first cavity stable; when the cooling and heating device cools one side of the first cavity, the heat generated on one side of the second cavity can be discharged out of the second cavity through the second fan and the second heat sink to prevent the heat from being transferred to the first cavity, thereby keeping the temperature in the first cavity stable, thereby improving the separation accuracy of the chromatographic column.

[0015] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings constituting a part of this document are used to provide a further understanding of this document. The exemplary embodiments and descriptions of this document are used to explain this document and do not constitute an improper limitation on this document. In the accompanying drawings: Figure 1It is a structural schematic diagram of a column temperature box (with a hidden door body and part of the box body) with bidirectional temperature control provided by an exemplary embodiment of the present application; Figure 2 is a cross-sectional view of a column oven capable of bidirectional temperature control provided by an exemplary embodiment of the present application; Figure 3 yes Figure 2 The enlarged view in the middle dashed box; Figure 4 It is a schematic diagram of the structure of a column temperature oven capable of bidirectional temperature control provided by an exemplary embodiment of the present application.

[0017] In the figure: 10. Box body; 101. Upper partition; 102. Lower partition; 103. Third fan; 104. Fourth fan; 20. Door body; 201. First layer door body; 202. Second layer door body; 30. Inner tank; 301. Adapter; 3011. First groove; 3012. Second groove; 302. Fixed chromatographic column housing; 303. Air guide housing; 304. Insulation board; 40. Refrigeration and heating device; 50. First fan; 60. First heat sink; 70. Second fan; 80. Second heat sink; 100A. First cavity; 100B. Middle cavity; 100C. Upper cavity; 100D. Lower cavity. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application. It should be noted that, in the absence of conflict, the embodiments in the present application and the features in the embodiments can be combined with each other arbitrarily.

[0019] The temperature of the liquid chromatography column has a great influence on the adsorption analysis results of the components therein. The temperature change and the temperature difference in the column oven will affect the accuracy of the separation results of the chromatographic column.

[0020] In a traditional column oven, due to unstable air circulation in the oven, the heat or cold load in the oven will be transferred to the reaction chamber of the chromatographic column, resulting in unstable temperature in the reaction chamber of the chromatographic column, which in turn causes low accuracy of the chromatographic column separation results.

[0021] Based on this, an exemplary embodiment of the present application provides a column temperature box with bidirectional temperature control, including a first cavity formed by an inner tank, a cooling and heating device and a door body, and a second cavity formed by an inner tank, a cooling and heating device and a box body. A first fan and a first heat sink are arranged in the first cavity, and a second fan and a second heat sink are arranged in the second cavity. In this way, when the cooling and heating device heats one side of the first cavity, the cold load generated on one side of the second cavity can be discharged out of the second cavity through the second fan and the second heat sink to prevent the cold load from being transferred to the first cavity, thereby keeping the temperature in the first cavity stable; when the cooling and heating device cools one side of the first cavity, the heat generated on one side of the second cavity can be discharged out of the second cavity through the second fan and the second heat sink to prevent the heat from being transferred to the first cavity, thereby keeping the temperature in the first cavity stable, thereby improving the separation accuracy of the chromatographic column.

[0022] An exemplary embodiment of the present application provides a column oven capable of bidirectional temperature control, such as Figure 1 and 4 As shown, the column temperature box includes a box body 10 and a door body 20. The box body 10 and the door body 20 enclose a cavity, and the box body 10 is provided with air inlet and outlet holes. An inner liner 30 and a cooling and heating device 40 are provided in the cavity. The cooling and heating device 40 is preferably a Peltier cooling and heating plate. According to the reaction temperature requirements, the cooling and heating device 40 can perform cooling or heating to adjust the reaction temperature within the range of 5°C and 90°C. The inner tank 30 is fixedly arranged on the box body 10, and the cooling and heating device 40 is fixedly arranged on the side of the inner tank 30 away from the door body 20; the inner tank 30, the cooling and heating device 40 and the door body 20 enclose a closed first cavity 100A; the inner tank 30, the box body 10 and the cooling and heating device 40 enclose a second cavity; the chromatographic column is arranged in the first cavity 100A; the first fan 50 and the first heat sink 60 are arranged in the first cavity 100A to drive the air to move in the first cavity 100A; the second fan 70 and the second heat sink 80 are arranged in the second cavity to drive the air to move in the second cavity.

[0023] like Figure 2As shown, the black bold arrows in the figure indicate the air circulation paths in the first cavity 100A and the second cavity. Preferably, the first fan 50 and the first heat sink 60 are both arranged near the middle position in the vertical direction of the first cavity 100A, and the first fan 50 and the first heat sink 60 are arranged at intervals, and the first heat sink 60 has a plurality of evenly arranged heat dissipation grooves that penetrate vertically up and down. When the cooling and heating device 40 heats one side of the first cavity 100A, the heat is transferred to the first cavity 100A through the heat sink, and the first fan 50 blows air toward the first heat sink 60. The moving airflow will move in the upper and lower directions from the heat sink, so that two upper and lower circulating airflows are formed in the first cavity 100A, so as to make the heat distribution of the first cavity 100A more uniform. At the same time, one side of the cooling and heating device 40 located in the second cavity will be cooled, and the generated cold load will be transmitted to the second cavity through the second heat sink 80. The second heat sink 80 has a plurality of evenly arranged heat dissipation grooves that run vertically up and down. The second fan 70 blows air to the second heat sink 80, so that two upper and lower circulating airflows are formed in the second cavity, which can quickly discharge the cold load generated by the refrigeration to the outside of the second cavity, prevent the generated cold load from being transmitted to the first cavity 100A, and affect the temperature in the first cavity 100A, so as to prevent the temperature in the first cavity 100A from fluctuating and affecting the separation accuracy of the chromatographic column.

[0024] When the cooling and heating device 40 cools one side of the first cavity 100A, exemplarily, the reaction temperature is 5°C. At this time, the cold load generated by the cooling and heating device 40 is transferred to the first cavity 100A through the first heat sink 60, and an up and down circulating airflow is formed in the first cavity 100A through the first fan 50, so as to make the cold load distribution in the first cavity 100A more uniform. At this time, the cooling and heating device 40 is heating on the second cavity side. If the heat generated in the second cavity is not discharged in time, the heat will be transferred to the first cavity 100A, making the temperature in the first cavity 100A unstable. At the same time, due to the heat transfer, the temperature in the first cavity 100A may not be able to reach the preset 5°C. In this way, the control system will always control the cooling and heating device 40 to cool. At the same time, the cooling and heating device 40 is always heating in the second cavity, and the heat generated by the heating will be always transferred to the first cavity 100A. This heat transfer will always cause the temperature in the first cavity 100A to be unable to reach the preset temperature, causing the cooling and heating device 40 to work all the time, with high energy consumption, and the separation accuracy of the chromatographic column cannot be guaranteed. In the present application, the heat generated on one side of the second cavity can be discharged out of the second cavity through the second fan 70 and the second heat sink 80 to prevent the heat from being transferred to the first cavity 100A. This can not only keep the temperature in the first cavity 100A stable, but also quickly reduce the temperature of the first cavity 100A to a preset temperature and maintain the preset temperature unchanged, thereby improving the separation accuracy of the chromatographic column.

[0025] An upper partition 101 and a lower partition 102 are provided in the second cavity, dividing the second cavity into an upper cavity 100C, a middle cavity 100B and a lower cavity 100D. In order to accelerate the air in the second cavity to be discharged to the outside of the box body 10, a third fan 103 and a fourth fan 104 are arranged in an upper and lower interval on one side of the middle cavity 100B away from the inner liner 30. The third fan 103 and the fourth fan 104 exhaust air to the outside of the box body 10 at the same time to accelerate the air in the middle cavity 100B to be discharged to the outside.

[0026] like Figure 3 As shown, the inner tank 30 includes an air guide housing 303, a fixed chromatographic column housing 302 and an adapter 301; the adapter 301 is fixed to the box 10, the fixed chromatographic column housing 302 is fixedly connected to the side of the adapter 301 away from the door body 20, and the air guide housing 303 is fixedly connected to the side of the fixed chromatographic column housing 302 away from the door body 20. Figure 1As shown, the fixed chromatographic column housing 302 includes a mounting plate located in the middle of the first cavity 100A, and the mounting plate is provided with through holes in the shape of a long strip, an arc, etc., to facilitate the circulation of airflow in the first cavity 100A; at the same time, a chromatographic column clamp is also provided on the side of the mounting plate close to the door body 20, and the chromatographic column is fixed on the chromatographic column clamp; the first fan 50 is installed on the side of the mounting plate away from the door body 20. Preferably, a plurality of long strip and arc-shaped through holes are provided at the position where the first fan 50 is installed on the mounting plate, and these through holes can facilitate the circulating airflow to enter the first fan 50, so that the air circulation in the first cavity 100A is stable and the temperature distribution is more uniform.

[0027] The outer portion of the inner liner 30 is covered with a heat preservation board 304. Preferably, the top, side, bottom and the area near the second cavity of the inner liner 30 are all covered with a heat preservation board 304 to prevent the heat or cold load in the second cavity from being transferred to the first cavity 100A. The heat preservation board 304 is preferably made of EVA material.

[0028] In one embodiment, in order to facilitate the reverse circulation of air in the first cavity 100A and make the temperature in the first cavity 100A more uniform, the air guide housing 303 includes an inclined air guide surface, such as Figure 3 As shown in the figure, the setting of the upper and lower air guide surfaces makes the opening of the first cavity 100A gradually increase from the cooling and heating device 40 toward the door body 20. Therefore, the air guide surface can guide the air movement in the main cavity and accelerate the circulation of the air in the main cavity. Under the action of the first fan 50, the airflow emitted from the upper and lower ends of the first heat sink 60 turns at the air guide surface to flow toward the door body 20, so that circulating airflow is formed in the upper and lower parts of the first cavity 100A, which can make the temperature in the first cavity 100A more uniform.

[0029] like Figure 2 and 3As shown, one side of the adapter 301 is in contact with the housing 10 and the door 20, and the other side is in contact with the fixed chromatographic column housing 302. Preferably, the adapter 301 is made of ABS. On the basis of having a certain strength, the thermal conductivity of ABS is lower than that of metal materials. Therefore, it can prevent the formation of a cold bridge between the fixed chromatographic column housing 302, the housing, and the door 20, and avoid the loss of more heat or cold load. A first groove 3011 and a second groove 3012 are connected to each other on one side of the adapter 301 close to the door 20. The first groove 3011 and the second groove 3012 together form a convex groove facing one side of the fixed chromatographic column housing 302. A plurality of mounting holes for realizing threaded connection with the fixed chromatographic column housing 302 are spaced apart in the first groove 3011, and a sealing member is provided in the convex groove. In this way, the fastener is sealed in the groove by the seal, which can prevent the fastener from contacting the door body 20 and the box body 10 to form a cold bridge, reduce the transfer of heat or cold load, and maintain a stable temperature in the first cavity 100A. At the same time, the side of the seal that enters the door body 20 will slightly protrude from the outside of the groove. Therefore, when the door body 20 is closed, the door body 20 squeezes the seal, and a close contact is formed between the door body 20 and the seal, which improves the sealing between the first cavity 100A and the door body 20, and reduces the loss of heat or cold load in the first cavity 100A.

[0030] The upper partition 101 and the lower partition 102 arranged in the second cavity can prevent the temperature of the upper cavity 100C and the lower cavity 100D from being too low or too high, and avoid the excessively high or too low temperature from affecting the regulating valve arranged in the upper cavity 100C and the lower wall. The outer surface of the insulation board 304 is also covered with a cover plate, and fasteners are threadedly connected to the upper partition 101 and the lower partition 102, and the ends of the fasteners are in contact with the cover plate to apply a pressing force to the inner tank 30 in the direction of the door body 20, so as to further seal the first cavity 100A. The insulation board 304 has a certain elasticity, so by adjusting the length between the fastener and the cover plate, the pressing force of the fastener on the cover plate can be adjusted, so that the inner tank 30 and the door body 20 are further sealed.

[0031] In one embodiment, if Figure 3 As shown, the door body 20 is a double-layer door body 20, which includes a first-layer door body 201 and a second-layer door body 202. The first-layer door body 201 is sealed on the outside of the first cavity 100A, and the second-layer door body 202 is sealed on the outside of the first-layer door body 201. The second-layer door body 202 seals the upper cavity 100C and the lower cavity 100D at the same time. In this way, when it is necessary to operate the regulating valves arranged in the upper cavity 100C and the lower cavity 100D, it is only necessary to open the second-layer door body 202 without opening the first-layer door body 201, thereby ensuring the sealing of the first cavity 100A and avoiding heat transfer between the first cavity 100A and the outside world, thereby affecting the temperature inside the first cavity 100A.

[0032] In one embodiment, a first temperature sensor and a second temperature sensor are provided in the inner tank 30, the first temperature sensor is provided on one side of the first fan 50, and the second temperature sensor is provided in the first heat sink 60. The temperature of the heat sink and the temperature of the air in the first cavity 100A are detected at the same time, and the control system can accurately control the temperature in the first pump cavity according to the data information collected by the first temperature sensor and the second temperature sensor, thereby improving the separation accuracy of the chromatographic column.

[0033] In order to further improve the measurement accuracy of the first temperature sensor and the second temperature sensor, exemplarily, the first temperature sensor and the second temperature sensor both adopt Kelvin-free sensors, that is, the outer shells of the first temperature sensor and the second temperature sensor are removed. Because the outer shell can affect the detection accuracy of the sensor, the first temperature sensor and the second temperature sensor with the outer shell removed can measure more accurately; at the same time, in order to increase the heat conduction efficiency, copper foil is coated on the outside of the first temperature sensor and the second temperature sensor. Due to the high heat conduction efficiency of copper foil, the detection sensitivity of the first temperature sensor and the second temperature sensor can be improved, thereby further improving the temperature accuracy in the first cavity 100A.

[0034] The temperature adjustment range of the column oven of the present application is 5°C to 90°C, the temperature accuracy can reach 0.01, and the temperature stability can reach ±0.05°C; when set to 35°C, the heating time is 2min.

[0035] In this application, the terms "comprises", "comprising" or any other variations thereof are intended to cover non-exclusive inclusion, so that an article or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such article or device. In the absence of more restrictions, the elements defined by the sentence "comprising..." do not exclude the presence of other identical elements in the article or device comprising the elements.

[0036] Although the preferred embodiments of the present application have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications falling within the scope of the present application.

[0037] Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the intention of the present application also includes these modifications and variations.

Claims

1. A column oven with bidirectional temperature control, It is characterized in that It comprises a box body and a door body, wherein the box body and the door body enclose a cavity; An inner tank and a cooling and heating device are arranged in the cavity, the inner tank is fixedly arranged on the box body, and the cooling and heating device is fixedly arranged on a side of the inner tank away from the door body; The inner tank, the refrigeration and heating device and the door body form a closed first cavity; the inner tank, the box body and the refrigeration and heating device form a second cavity; the chromatographic column is arranged in the first cavity; A first fan and a first heat sink are disposed in the first cavity to drive air to move in the first cavity; a second fan and a second heat sink are disposed in the second cavity to drive air to move in the second cavity.

2. The column oven capable of bidirectional temperature control according to claim 1, It is characterized in that The inner tank includes an air guide shell, a fixed chromatographic column shell and an adapter; the adapter is fixed on the box body, the fixed chromatographic column shell is fixedly connected to the side of the adapter away from the door body, and the air guide shell is fixedly connected to the side of the fixed chromatographic column shell away from the door body.

3. The column oven capable of bidirectional temperature control according to claim 2, It is characterized in that The air guide housing includes an inclined air guide surface, which can guide the movement of air in the main cavity and accelerate the circulation of air in the main cavity.

4. The column oven capable of bidirectional temperature control according to claim 2, It is characterized in that A first groove and a second groove that are interconnected are provided on one side of the adapter close to the door body. The first groove and the second groove together form a convex groove facing the side of the fixed chromatographic column housing. A plurality of mounting holes for realizing a threaded connection with the fixed chromatographic column housing are spaced apart in the first groove.

5. The column oven capable of bidirectional temperature control according to claim 4, It is characterized in that A sealing member is arranged in the convex groove.

6. The column oven capable of bidirectional temperature control according to claim 1, It is characterized in that The outer portion of the inner container is covered with a heat preservation plate to prevent the heat or cold load in the second cavity from being transferred to the first cavity.

7. The column oven capable of bidirectional temperature control according to claim 2, It is characterized in that An upper partition and a lower partition are arranged in the second cavity, dividing the second cavity into an upper cavity, a middle cavity and a lower cavity.

8. The column oven capable of bidirectional temperature control according to claim 4, It is characterized in that A third fan and a fourth fan are arranged in an upper and lower spaced relationship in the middle cavity at one side away from the inner container.

9. The column oven capable of bidirectional temperature control according to claim 1, It is characterized in that A first temperature sensor and a second temperature sensor are arranged in the inner tank, the first temperature sensor is arranged on one side of the first fan, and the second temperature sensor is arranged in the first heat sink.

10. The column oven capable of bidirectional temperature control according to claim 6, It is characterized in that The outside of the insulation board is also covered with a cover plate, and fasteners are threadedly connected to the upper and lower partitions. The ends of the fasteners abut against the cover plate to apply a pressing force to the inner tank in the direction of the door body to further seal the first cavity.

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