Preparation method of radix aconiti carmichaeli processed product with anti-inflammatory effect

Abstract

The invention provides a preparation method of a radix aconiti carmichaeli processed product with an anti-inflammatory effect, and belongs to the technical field of traditional Chinese medicine decoction piece processing methods. The preparation method comprises the following steps: mixing auxiliary materials with distilled water, pulping, filtering, collecting filtrate, soaking raw radix aconiti lateralis praeparata slices in the filtrate, boiling the soaked radix aconiti lateralis praeparata slices in boiled rice water, and drying. The auxiliary materials comprise rice water, ginger and clam meal. The preparation method provided by the invention is simple, and effective components of the prepared radix aconiti carmichaeli processed product are greatly reserved, so that the prepared radix aconiti carmichaeli processed product has a relatively good anti-inflammatory effect.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicine preparation methods, and in particular to a method for preparing a prepared aconite root with anti-inflammatory effect. Background Art

[0002] The Chinese herbal medicine Fuzi is a processed product of the root of the Ranunculaceae plant Aconitum. It is used for yang deficiency, collapse, cold limbs and weak pulse, insufficient heart yang, chest pain, vomiting and diarrhea due to deficiency and cold, cold pain in the abdomen, kidney yang deficiency, impotence and cold uterus, edema due to yin cold, exogenous infection due to yang deficiency, and pain due to dampness and cold. Fuzi is pungent, sweet, hot, and toxic. It is very harmful to health if used without processing. Therefore, Chinese medicine generally uses processing methods to reduce its toxic side effects.

[0003] There are many processed products of Radix Aconiti Lateralis, but the Chinese Pharmacopoeia and most regions do not make a distinction in the efficacy and indications of all processed varieties of Radix Aconiti Lateralis, and all are recorded as returning yang to rescue adverse conditions, replenishing fire and assisting yang, dispelling cold and relieving pain, etc. In terms of clinical application, it is not clear what the characteristics of each processed product are, how the efficacy differences are, and how to select drugs according to the syndrome, which may affect the clinical rational use of processed products of Radix Aconiti Lateralis to a certain extent. Various processed products of Radix Aconiti Lateralis currently available on the market, such as commercially available Heishun Tablets, commercially available Baifu Tablets, commercially available Paofu Tablets, etc., all have the disadvantages of few effective ingredients and unobvious effects, indicating that these processed products of Radix Aconiti Lateralis have a large amount of chemical components lost during processing. Therefore, it is necessary to provide a new processing method of Radix Aconiti Lateralis. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a method for preparing a processed aconite with anti-inflammatory effect. The processed aconite obtained by the preparation method provided by the present invention has less loss of effective ingredients.

[0005] In order to achieve the above-mentioned purpose, the present invention provides the following technical scheme: a preparation method of a processed aconite with anti-inflammatory effect, comprising the steps of mixing auxiliary materials with distilled water, pulping and filtering, collecting the filtrate, soaking the raw aconite slices in the filtrate, putting the soaked aconite slices into boiling rice water, cooking and drying; the auxiliary materials are rice water, ginger and clam powder.

[0006] In some embodiments, the ginger is ginger.

[0007] In some embodiments, the mass ratio of the rice water, ginger, and clam powder is (1-2):(1-2):(1-2), preferably 1:1:1.

[0008] Preferably, the mass ratio of the auxiliary material to distilled water is 1:(5-7), preferably 1:5.

[0009] Preferably, the mass ratio of the raw aconite root to the filtrate is (13-15):1, preferably 15:1.

[0010] Preferably, the soaking time is 2-4 hours, preferably 2 hours.

[0011] Preferably, the cooking time is 4-7 hours, preferably 6 hours.

[0012] Preferably, the drying temperature is 35-45°C, preferably 39°C.

[0013] Beneficial technical effects: The present invention provides a method for preparing a processed aconite with anti-inflammatory effects. The method comprises mixing auxiliary materials with distilled water, beating and filtering, collecting the filtrate, soaking the raw aconite in the filtrate, and putting the soaked aconite into boiling rice water for cooking and drying; the auxiliary materials are rice water, ginger, and clam powder. The preparation method provided by the present invention is simple, and the effective ingredients of the processed aconite prepared are largely retained, and the total content of three monolipid alkaloids (MDAs) is high, close to 3.5 mg / g, far exceeding the pharmacopoeia standard and four commercially available aconite, and has a good anti-inflammatory effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a comparison chart of the contents of three kinds of DDAs and three kinds of MDAs in the processed aconite root product prepared in Example 1 and different processed aconite root products;

[0015] Figure 2 The figure is a comparison chart of 11 chemical components of the processed aconite root product prepared in Example 1 and different processed aconite root products;

[0016] Figure 3 This is a graph showing the effects of the processed aconite root product prepared in Example 1 and different processed aconite root products on the NO inhibition rate in RAW264.7 cells, *P<0.05, **P<0.01, ****P<0.0001 vs. model group, n=3. DETAILED DESCRIPTION

[0017] In order to better understand the present invention, the content of the present invention is further explained below in conjunction with the embodiments, but the content of the present invention is not limited to the following embodiments.

[0018] Example 1

[0019] Prepare rice water, ginger and clam powder into auxiliary materials in a mass ratio of 1:1:1, mix the auxiliary materials with distilled water in a mass ratio of 1:5, beat and filter, collect the filtrate, soak the raw aconite in the filtrate for 2 hours in a mass ratio of raw aconite to filtrate of 15:1, put the soaked aconite into boiling rice water, cook for 6 hours, and dry at 39°C.

[0020] Example 2

[0021] Prepare rice water, ginger and clam powder into auxiliary materials in a mass ratio of 1:2:1, mix the auxiliary materials with distilled water in a mass ratio of 1:7, pulp and filter, collect the filtrate, soak the raw aconite in the filtrate for 3 hours in a mass ratio of raw aconite to filtrate of 13:1, put the soaked aconite into boiling rice water, cook for 4 hours, and dry at 35°C.

[0022] Example 3

[0023] Prepare rice water, ginger and clam powder into auxiliary materials in a mass ratio of 2:2:1, mix the auxiliary materials with distilled water in a mass ratio of 1:6, pulp and filter, collect the filtrate, soak the raw aconite in the filtrate for 4 hours in a mass ratio of raw aconite to filtrate of 14:1, put the soaked aconite into boiling rice water, cook for 7 hours, and dry at 45°C.

[0024] Test Example 1

[0025] Determination of the content of active chemical components in processed products of Radix Aconiti Lateralis Preparata

[0026] The HPLC-DAD-ELSD method was used to establish the content determination method of three diester alkaloids and three monoester alkaloids. The chromatographic conditions are as follows:

[0027] The chromatographic column was a Phenomenex Gemini C18 column (4.6 mm × 150 mm, 5 μm); the mobile phase was 0.02% ammonium acetate (A)-acetonitrile (B) gradient elution. The flow rate was 1 mL / min, the column temperature was 30 °C, the injection volume was 20 μL, the detection wavelength was 240 nm; the carrier gas was N 2 , evaporation temperature is 50℃, carrier gas flow rate is 1.5L / min, and gain is 1.

[0028] The test solution was prepared as follows:

[0029] Accurately weigh 3 parts of raw aconite powder (embodiment 1) 2g (pass through No. 3 sieve), place in a 250mL conical flask, add 15mL of methanol, 15mL of 0.05% hydrochloric acid aqueous solution, seal and soak for 1h before ultrasonic treatment for 30min (power 1300W, frequency 40kHz), centrifuge at 10000r / min for 10min, take 20mL of supernatant in an eggplant-shaped flask, swirl to 0.5-1mL at 38°C under reduced pressure, make the volume to 5mL volumetric flask with 0.05% hydrochloric acid aqueous solution, and filter with 0.22μm microporous membrane.

[0030] Take 20 μL of the test sample and inject it into the chromatographic column for detection. The results are as follows: Figure 2 , 3 shown.

[0031] Depend on Figure 1It can be seen that the total amount of the three di-ester alkaloids (aconitine, meaconitine and aconitine, DDAs) in Example 1 is lower than the pharmacopoeia standard, indicating that its safety may be good. The total amount of the three mono-ester alkaloids (benzoylaconitine, benzoylmeaconitine, benzoylhypaconitine, MDAs) is relatively high, close to 3.5 mg / g, far exceeding the pharmacopoeia standard and the four commercially available aconite slices. In summary, based on the content standards of the six ester alkaloids specified in the pharmacopoeia (the content of the three DDAs is used to evaluate its safety, and the content of the three MDAs is used to evaluate its effectiveness), the prepared aconite root product prepared in Example 1 meets the safety standards and has better effectiveness. Figure 2 It can be seen that the total content of 11 chemical components of the processed aconite prepared in Example 1 is much higher than that of 4 commercially available aconite slices. In addition, the types of chemical components of commercially available Heishunpian, Baifupian and Paofupian are also very few, suggesting that the chemical component contents of these 3 aconite slices are lost in large quantities during processing.

[0032] The results of the content of active ingredients showed that the contents of the three DDAs in the processed aconite of Example 1 reached the standards of the Chinese Pharmacopoeia, and the contents of the three MDAs and the total amount of 11 active ingredients far exceeded those of the aconite slices prepared by the traditional processing method on the market.

[0033] Test Example 2

[0034] In vitro anti-inflammatory assay

[0035] The RAW264.7 cell inflammation model stimulated by LPS and IFN-γ was used, and celecoxib, a drug that can effectively relieve inflammation and relieve pain, was selected as a positive drug. The nitrate reductase method was used to detect the content of inflammatory mediator NO in the supernatant of RAW264.7 macrophages under inflammatory conditions, and the NO inhibition rate was calculated to evaluate the in vitro anti-inflammatory effects of different processed products of Aconite. Figure 3 As shown, at the same dose, the NO inhibition rate of the processed aconite of Example 1 is better than that of the commercially available aconite recorded in the pharmacopoeia, and has the highest NO inhibition rate, and is much higher than that of the positive drugs, indicating that the aconite prepared with ginger + rice water + clam powder may have a better anti-inflammatory effect; the NO inhibition rate of the commercially available light aconite is the lowest, indicating that its anti-inflammatory effect may be weaker.

[0036] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a processed aconite product with anti-inflammatory effect, characterized in that: The auxiliary materials are mixed with distilled water, then pulped and filtered, the filtrate is collected, the raw aconite root is soaked in the filtrate, and the soaked aconite root is put into boiling rice water for cooking and drying; the auxiliary materials are rice water, ginger and clam powder.

2. The preparation method according to claim 1, characterized in that: The ginger is fresh ginger.

3. The preparation method according to claim 1, characterized in that: The mass ratio of the rice water, ginger and clam powder is (1-2):(1-2):(1-2).

4. The preparation method according to claim 1, characterized in that: The mass ratio of the auxiliary material to distilled water is 1:(5-7).

5. The preparation method according to claim 1, characterized in that: The mass ratio of the raw aconite root to the filtrate is (13-15):

1.

6. The preparation method according to claim 1, characterized in that: The soaking time is 2-4 hours.

7. The preparation method according to claim 1, characterized in that: The cooking time is 4-7 hours.

8. The preparation method according to claim 1, characterized in that: The drying temperature is 35-45°C.

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