Nucleic acid sample pretreatment device

Abstract

The invention provides a nucleic acid sample pretreatment device. The nucleic acid sample pretreatment device comprises a box body, and a sample cracking mechanism, a cover opening and closing mechanism, a nucleic acid extraction mechanism and a liquid relief mechanism which are arranged in the box body. Wherein the sample cracking mechanism comprises a sample frame and a cracking assembly; the sample rack is provided with a plurality of sample through holes for placing a plurality of sample tubes; the splitting assembly is provided with a splitting station and is used for performing cell nucleus splitting on a sample in the sample tube extending into the splitting station; the cover opening and closing mechanism comprises a first driving part and a cover opening part, and the first driving part drives the cover opening part to open a tube cover of the sample tube after the cell nucleus splitting procedure; the nucleic acid extraction mechanism is used for performing nucleic acid extraction operation on the split nucleic acid sample; the pipetting mechanism comprises a movable sampling gun, the sampling gun provided with a gun head moves to the uncovered sample tube to extract a sample and moves to the nucleic acid extraction mechanism to discharge the sample, full-closed automatic operation is achieved, the working efficiency is improved, personal errors are reduced, and the accuracy and reliability of experimental results are ensured.

Description

Technical Field

[0001] The present invention relates to the field of biochemical detection equipment, and in particular to a nucleic acid sample pre-treatment device. Background Art

[0002] Modern medical industry inspections require the completion of various inspection items. Before the sample enters the inspection equipment, it needs to go through a series of processing work, including loading, uncapping, transfer, etc. In the prior art, the functions of traditional nucleic acid sample pre-treatment devices are relatively single, and generally require multiple devices and multiple people to complete these pre-inspection tasks (hereinafter referred to as pre-treatment work). The work is cumbersome, the process is complicated, and the efficiency is low. The automation requirements for medical inspection work are becoming increasingly obvious, and various automatic inspection lines have also become an urgent need for the medical industry. The premise of inspection automation is the ability to perform efficient batch sample pre-processing work.

[0003] Therefore, how to provide a reliable and efficient nucleic acid sample pretreatment device is a technical problem that urgently needs to be solved in this field. Summary of the invention

[0004] The main technical problem solved by the present invention is to provide a reliable and efficient nucleic acid sample pretreatment device.

[0005] The present invention provides a nucleic acid sample pre-treatment device, comprising:

[0006] Box;

[0007] The sample lysis mechanism comprises a sample rack and a lysis assembly; the sample rack has a plurality of sample through holes for placing a plurality of sample tubes; the lysis assembly has a lysis station for performing cell nucleus lysis on the sample in the sample tube extending into the lysis station;

[0008] The cover opening and closing mechanism comprises a first driving member and a cover opening member. After the cell nucleus lysis process, the first driving member drives the cover opening member to open the tube cover of the sample tube;

[0009] A nucleic acid extraction mechanism, used for performing nucleic acid extraction operations on the lysed nucleic acid sample;

[0010] and a liquid transfer mechanism, including a movable sampling gun, wherein the sampling gun equipped with a gun head moves to the opened sample tube to extract the sample, and moves to the nucleic acid extraction mechanism to discharge the sample;

[0011] Among them, the sample lysis mechanism, the cover opening and closing mechanism, the nucleic acid extraction mechanism and the liquid transfer mechanism are all arranged in the box.

[0012] In an optional embodiment, the sample rack is arranged above the lysis assembly, and a plurality of sample through holes are projected into the lysis station; the sample lysis mechanism further comprises a positioning assembly mounted on the sample rack to fix a plurality of sample tubes;

[0013] The sample lysis mechanism also includes a first lifting component, which is transmission-connected to the sample rack or the lysis component so that the sample rack and the lysis component move closer to or farther from each other in the vertical direction, so that the sample tube can extend into the lysis station or be separated from the lysis station.

[0014] In an optional embodiment, the sample rack includes a mounting base and a sample plate, and the mounting base and the sample plate slide together in a first direction to form a drawer-type sample rack; the first direction is perpendicular to the vertical direction, the sample rack is fixedly installed in the box body through the mounting base, the positioning assembly is installed on the sample plate, the sample through hole is located on the sample plate, and the first lifting assembly is transmission-connected to the mounting base to drive the mounting base, the sample plate and the positioning assembly to rise and fall as a whole in the vertical direction.

[0015] In an optional embodiment, a first window is provided on the box body for the sample plate and the positioning assembly to be drawn out of the box body along a first direction, and the box body also has a first sealing plate for closing the first window.

[0016] In an optional embodiment, the nucleic acid extraction mechanism includes a slide rail, a consumables platform and a nucleic acid extraction component, the consumables platform is used to place consumables such as a reagent kit, and the nucleic acid extraction component is used to complete nucleic acid washing and elution in the reagent kit;

[0017] The slide rail is arranged on the box body, and the consumable platform and the slide rail slide together along the first direction to form a drawer-type platform; along the first direction, the consumable platform and the nucleic acid extraction component are arranged relatively to each other, and the nucleic acid extraction component is located on the push-back side of the consumable platform. The first direction is perpendicular to the vertical direction. The nucleic acid extraction component has an extraction component for contacting the nucleic acid sample, and the extraction component extends to the top of the consumable platform.

[0018] In an optional embodiment, the sample lysis mechanism and the nucleic acid extraction mechanism are arranged side by side along the second direction, the second direction is the width direction of the sample lysis mechanism and the width direction of the nucleic acid extraction mechanism, and the first direction, the second direction and the vertical direction are perpendicular to each other.

[0019] In an optional embodiment, the box body has a middle partition, which is Z-shaped and includes a first plate, a second plate and a third plate connecting the first plate and the second plate. The first plate and the second plate are spaced apart in the vertical direction, and the middle partition divides the box body into a first layer and a second layer which are distributed in sequence from bottom to top in the vertical direction; the sample lysis mechanism and the nucleic acid extraction mechanism are arranged on the first layer, the pipetting mechanism and the opening and closing cover mechanism are arranged on the second layer, the first plate is provided with a first connecting window for exposing the sample tube and a second connecting window for exposing the consumable platform, the consumable platform is located between the first plate and the box body, the nucleic acid extraction component is arranged between the second plate and the box body, and the third plate is provided with a third connecting window for allowing the extraction component to extend above the consumable platform and move.

[0020] In an optional embodiment, a second window is provided on the box body for the consumables platform to be pulled out of the box body, and the box body also has a second sealing plate for closing the second window.

[0021] In an optional embodiment, the nucleic acid sample pretreatment device also includes a moving arm, and the pipetting wall is arranged on the box body and is located on the second layer; the pipetting mechanism and the opening and closing cover mechanism are driven by the moving arm to move in a plane perpendicular to the vertical direction; the first driving member includes a rotating component that drives the clamp to rotate and a second lifting component that drives the clamp to rise and fall in the vertical direction; the pipetting mechanism also includes a third lifting component that drives the sampling gun to rise and fall in the vertical direction.

[0022] In an optional embodiment, a placement portion for placing multiple gun tips is provided in the box; the sampling gun has a connecting structure for installing the gun tip, the sampling gun and the gun tip are detachably connected via the connecting structure, and the sampling gun is moved to the placement portion to replace the gun tip.

[0023] According to the nucleic acid sample pre-processing device of the above embodiment, it includes a sample lysis mechanism, an opening and closing cover mechanism, a liquid transfer mechanism and a nucleic acid extraction mechanism, and has the functions of sample tube loading, lysis, mixing, sample tube opening and closing cover, liquid transfer and nucleic acid extraction, and can efficiently complete a series of pre-test processing work such as sample lysis, mixing, transfer, nucleic acid extraction, etc., and can be used as a pre-test processing device alone. And the sample lysis mechanism, the opening and closing cover mechanism, the nucleic acid extraction mechanism and the liquid transfer mechanism are all encapsulated in the box body, and only the sample tube needs to be manually placed in the sample rack, and then the box body is closed, and the subsequent processing work is fully closed and automatically operated, which improves work efficiency, reduces human errors, and ensures the accuracy and reliability of experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 A schematic diagram of the overall structure of a nucleic acid sample pretreatment device provided in some embodiments of the present invention.

[0025] Figure 2 for Figure 1 Schematic diagram of the explosion of the nucleic acid sample pretreatment device.

[0026] Figure 3 for Figure 1 Schematic diagram of the internal structure of the nucleic acid sample pretreatment device.

[0027] Figure 4 for Figure 1 Schematic diagram of the internal structure of the box of the nucleic acid sample pretreatment device.

[0028] Figure 5 for Figure 1 Schematic diagram of the structure of the nucleic acid sample pretreatment device after the box is omitted.

[0029] Figure 6A schematic diagram of the structure of a sample lysis mechanism in a nucleic acid sample pretreatment device provided in some embodiments of the present invention.

[0030] Figure 7 for Figure 6 Schematic diagram of the sample lysis mechanism from another perspective.

[0031] Figure 8 for Figure 6 Schematic diagram of the explosion of the sample cracking mechanism.

[0032] Fig. 9 for Figure 6 Schematic diagram of the connection between the positioning component of the sample lysis mechanism and the sample plate.

[0033] Fig.10 for Fig. 9 Schematic diagram of the explosion.

[0034] Fig.11 for Fig. 9 Front view of the positioning assembly in .

[0035] Fig.12 for Fig. 9 Further exploded diagram of the positioning component in

[0036] Fig.13 for Figure 6 Schematic diagram of a first lifting assembly of a sample lysis mechanism.

[0037] Fig.14 Schematic diagram of the overall structure of the liquid transfer mechanism, cover opening and closing mechanism and moving arm in the nucleic acid sample pretreatment device provided in some embodiments of the present invention.

[0038] Fig.15 for Fig.14 Schematic diagram of the structure of the pipetting mechanism and the opening and closing cover mechanism.

[0039] Fig.16 A schematic diagram of the structure of a nucleic acid extraction mechanism in a nucleic acid sample pretreatment device provided in some embodiments of the present invention

[0040] Fig.17 An exploded schematic diagram of a nucleic acid extraction mechanism and a limiting assembly in a nucleic acid sample pre-processing device provided in some embodiments of the present invention.

[0041] Fig.18 for Fig.17 Schematic diagram from another perspective.

[0042] Fig.19 It is a partial structural schematic diagram of the limiting component in the figure.

[0043] Fig. 20 for Fig.16Schematic diagram of the structure of the extraction component of the nucleic acid extraction mechanism.

[0044] Fig.21 for Fig. 20 Schematic diagram of the partial structure of the extraction component in .

[0045] Reference numerals: 100 - nucleic acid sample pretreatment device; 1 - sample tube; 2 - reagent box; 3 - eight-connecting tube; 4 - stock solution tube; 5 - reagent bottle;

[0046] 10-box; 11-first window; 12-second window; 13-first sealing plate; 14-second sealing plate; 15-middle partition; 151-first plate; 152-second plate; 153-third plate; 154-first connecting window; 155-second connecting window; 156-third connecting window; 16-first layer; 17-second layer; 18-first isolation plate;

[0047] 20-sample lysis mechanism; 21-sample rack; 211-sample through hole; 212-mounting seat; 213-sample plate; 214-loading plate; 215-base; 216-U-shaped seat; 22-lysis assembly; 221-lysis platform; 222-ultrasonic generator; 223-lysis chamber; 23-positioning assembly; 231-second driving member; 232-clamping member; 233-mounting frame; 234-mounting hole; 235-action part; 236-motor; 237-rotating wheel; 238-second slide rail; 239-sliding hole; 24-first lifting assembly; 241-support plate; 242-lifting fixed plate; 243-third driving member; 244-guide rail; 25-baffle;

[0048] 30-opening and closing cover mechanism; 31-first driving member; 311-rotating assembly; 312-second lifting assembly; 32-opening member;

[0049] 40-nucleic acid extraction mechanism; 41-first slide rail; 42-consumables platform; 421-placing part; 422-heating support; 43-nucleic acid extraction assembly; 431-extraction component; 432-magnetic rod group; 433-magnetic rod set; 434-translational drive assembly; 435-fourth lifting assembly; 436-fifth lifting assembly; 44-second isolation plate; 45-positioning member; 46-preload member; 47-stop block;

[0050] 50-liquid transfer mechanism; 51-sampling gun; 52-gun head; 53-third lifting assembly;

[0051] 60-movable arm; 70-limiting assembly; 71-base; 72-electromagnet, 73-ball lock; 731-male buckle; 732-female buckle, 74-induction switch. DETAILED DESCRIPTION

[0052] The present invention is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are for making the present application better understood. However, those skilled in the art can easily recognize that some features can be omitted in different situations, or can be replaced by other elements, materials, methods. In some cases, some operations related to the present application are not shown or described in the specification, this is to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations according to the description in the specification and the general technical knowledge in the art.

[0053] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a required sequence, unless otherwise specified that a certain sequence must be followed.

[0054] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0055] In the relevant technology, the pre-treatment of nucleic acid samples includes the processes of sample lysis, transfer and nucleic acid extraction. The downstream applications and tests after nucleic acid extraction can help us study the DNA or RNA in biological samples in more depth and reveal their important roles in biology, medicine and other fields. Common downstream applications and tests mainly include the following: 1. PCR amplification: amplify the target fragment from the extracted DNA or RNA for molecular diagnosis, molecular biology research, drug development, virus detection, etc. 2. Real-time fluorescence quantitative PCR: used for quantitative detection of DNA or RNA content, which can be used for genotype detection, differential expression analysis, cell number, viral load, genetically modified organism detection, etc. 3. Gene sequencing: sequence the extracted DNA or RNA for tumor genomics, newborn screening, precision medicine and genetic diagnosis, etc. 4. Protein research: use the extracted DNA or RNA to express and translate the synthesized protein for studying protein structure and function, etc.

[0056] In the prior art, it is generally necessary for multiple devices and multiple people to jointly complete the nucleic acid sample pre-treatment work before these tests, which has the problems of cumbersome work, complicated processes and low efficiency. In order to provide a highly automated, efficient and reliable nucleic acid sample pre-treatment device to meet the needs of medical testing work, the inventive concept of this application is to modularize and automate the various functional units of the nucleic acid sample pre-treatment device, and integrate each module in the box, so as to efficiently complete the sample loading, lysis, uncapping, sample transfer and nucleic acid extraction steps in the pre-treatment work, and can be used alone as a pre-test treatment device, which can improve work efficiency, reduce the time and labor intensity of manual operation, and ensure the accuracy and reliability of the test results.

[0057] In summary, the present invention is a fully automatic nucleic acid sample pretreatment device, which designs the internal layout and structure of the box body, and designs the distribution of the sample lysis mechanism, the cover opening and closing mechanism, the pipetting mechanism and the nucleic acid extraction mechanism in the box body, thereby achieving high integration and modularization, while reducing redundant motion components, and having a simple motion mode, which effectively reduces costs, and can efficiently complete a series of pre-inspection processing tasks such as sample lysis, mixing, transfer, nucleic acid extraction, etc., thereby realizing fully enclosed automatic operation of the processing process, and is easy to maintain, effectively reducing human errors, and ensuring the accuracy and reliability of the experimental results.

[0058] Please refer to Figure 1-Figure 5The present invention provides a nucleic acid sample pretreatment device 100, comprising a housing 10, a sample lysis mechanism 20, an opening and closing cover mechanism 30, a liquid transfer mechanism 50 and a nucleic acid extraction mechanism 40, wherein the sample lysis mechanism 20, the opening and closing cover mechanism 30, the nucleic acid extraction mechanism 40 and the liquid transfer mechanism 50 are all arranged in the housing 10, and only the sample tube 1 needs to be manually placed on the sample understanding mechanism, and then the housing 10 is closed, and the subsequent processing work is fully closed and automatically operated, which improves the work efficiency, reduces human errors, and ensures the accuracy and reliability of the experimental results. Specifically, the sample lysis mechanism 20 comprises a sample rack 21 and a lysis assembly 22; the sample rack 21 has a plurality of sample through holes 211 for placing a plurality of sample tubes 1; the lysis assembly 22 has a lysis station, and after the sample tube 1 is placed in the sample through hole 211, it can be inserted into the lysis station, and the lysis assembly 22 is used to perform cell nucleus lysis on the sample in the sample tube 1 inserted into the lysis station, and the sample rack 21 not only provides a loading place, but also provides a positioning place for the transfer of the sample. The cover opening and closing mechanism 30 includes a first driving member 31 and a cover opening member 32. After the cell nucleus lysis process, the first driving member 31 drives the cover opening member 32 to open the cover of the sample tube 1, so that the cover of the sample tube 1 remains closed during the lysis process to avoid leakage and contamination of the sample. In addition, after the lysis is completed, the cover of the sample tube 1 is opened by the cover opening and closing mechanism 30 so that the pipetting mechanism 50 can transfer the sample. The pipetting mechanism 50 includes a movable sampling gun 51, and the sampling gun 51 equipped with a gun head 52 moves to the sample tube 1 after the cover is opened to extract the sample, and moves to the nucleic acid extraction mechanism 40 to discharge the sample; the nucleic acid extraction mechanism 40 is used to perform nucleic acid extraction operations on the nucleic acid sample after lysis, and finally obtain the DNA or RNA of the sample in each sample tube 1 respectively.

[0059] According to the nucleic acid sample pre-treatment device 100 of the above embodiment, it includes a sample lysis mechanism 20, an opening and closing cover mechanism 30, a liquid transfer mechanism 50 and a nucleic acid extraction mechanism 40, and has the functions of loading, lysis, mixing, opening and closing the cover of the sample tube 1, liquid transfer and nucleic acid extraction, and can efficiently complete a series of pre-test processing work such as sample lysis, mixing, transfer, nucleic acid extraction, etc., and can be used as a pre-test processing device alone. And the sample lysis mechanism 20, the opening and closing cover mechanism 30, the nucleic acid extraction mechanism 40 and the liquid transfer mechanism 50 are all encapsulated in the box 10, and only the sample tube 1 needs to be manually placed on the sample rack 21, and then the box 10 is closed, and the subsequent processing work is fully closed and automatically operated, which improves work efficiency, reduces human errors, and ensures the accuracy and reliability of experimental results.

[0060] The function of the lysis component 22 is to lyse cells, release nucleic acids, and destroy the sample cell structure, so that the nucleic acids in the sample are free in the system. Common cell lysis methods include physical methods, chemical methods, and biological methods. Among them, the physical method refers to the rupture of cells by mechanical or physical action. Common methods include ultrasonic homogenization, high-pressure homogenization, glass grinding, etc.; the chemical method refers to the use of chemical reagents to destroy the cell membrane to achieve the effect of lysis, such as immersion method, freeze-thaw method, isotonic solution method, etc.; the biological method refers to the use of enzymatic hydrolysis of organisms such as microorganisms and enzymes for lysis. The lysis component 22 can be an automatic device for any of the above-mentioned feasible methods. This embodiment is not specifically limited, as long as it can cooperate with the sample rack 21 to achieve the effect of cell lysis.

[0061] In some embodiments, please refer to Figure 6-Figure 8 The lysis component 22 may include a lysis platform 221 and an ultrasonic generator 222. The lysis platform 221 has a lysis chamber 223 for accommodating a medium and a sample tube 1. The ultrasonic generator 222 is used to vibrate the medium in the lysis chamber 223, and utilizes the high-frequency vibration waves of ultrasonic waves in the medium to cause the sample liquid in the sample tube 1 to instantly undergo a large waveform change, thereby destroying the cell membrane, granulating the cells and releasing intracellular substances. At the same time, the vibration generated can also effectively mix the sample evenly, thereby making the various components in the sample more evenly distributed, reducing human errors, and helping to improve the accuracy of subsequent nucleic acid extraction.

[0062] In some embodiments, please refer to Figure 8 The sample rack 21 is arranged above the lysis assembly 22, and a plurality of sample through holes 211 are projected into the lysis station; the sample lysis mechanism 20 further comprises a first lifting assembly 24, which is transmission-connected to the sample rack 21 or the lysis assembly 22, so that the sample rack 21 and the lysis assembly 22 are moved closer to or farther away from each other in the vertical direction, so that the sample tube 1 can be extended into the lysis station or separated from the lysis station, so that there is enough operating space between the sample rack 21 and the lysis assembly 22 in the vertical direction without disassembly, so that the staff can maintain the sample rack 21 and the lysis assembly 22, or replace the liquid medium required by the lysis assembly 22, etc.

[0063] In some embodiments, please refer to Fig. 9 and Fig.10 The sample lysis mechanism 20 further includes a positioning assembly 23 installed on the sample rack 21 to fix a plurality of sample tubes 1, thereby ensuring that the positions of the sample tubes 1 are fixed during the lysis process.

[0064] Since the sample lysis mechanism 20 is encapsulated in the box 10, the box 10 needs to be opened when the sample tube 1 is loaded, and the sample tube 1 is placed in the sample through hole 211 of the sample rack 21. Considering the operability of loading, in some embodiments, the sample rack 21 may include a mounting seat 212 and a sample plate 213, please refer to Figure 8 , the mounting seat 212 and the sample plate 213 slide and cooperate in the first direction to form a drawer-type sample rack 21; the first direction is perpendicular to the vertical direction, the sample rack 21 is fixedly installed in the box 10 through the mounting seat 212, the positioning assembly 23 is installed on the sample plate 213, the sample through hole 211 is located on the sample plate 213, and the first lifting assembly 24 is connected to the mounting seat 212 to drive the mounting seat 212, the sample plate 213 and the positioning assembly 23 to rise and fall in the vertical direction as a whole. In other words, the sample rack 21 has the function of being movable in the first direction and the vertical direction. The setting of the drawer-type sample rack 21 allows the sample plate 213 to be pulled out of the box 10, which is convenient for manual loading. At the same time, the mounting seat 212 cooperates with the first lifting assembly 24, and by setting the sample lysis mechanism 20 to the initial state, the distance between the sample rack 21 and the lysis assembly 22 is larger. Therefore, after the sample tube 1 is placed on the sample plate 213, the sample plate 213 will not interfere with the lysis assembly 22 during the process of pushing the sample plate 213 back into place. After closing the box 10 and starting the entire equipment, closed and fully automatic processing can be carried out.

[0065] In some embodiments, in order to further optimize the nucleic acid sample pre-treatment device 100 and make the loading operation more convenient, please refer to Figure 2 The box body 10 may be provided with a first window 11 for the sample plate 213 and the positioning assembly 23 to be drawn out of the box body 10 along the first direction, and the box body 10 may also have a first sealing plate 13 for closing the first window 11. The first window 11 and the sample rack 21 are directly opposite in the first direction, and the sample plate 213 can be drawn out of the box body 10 after the first sealing plate 13 is removed, so that the sample through hole 211 is exposed outside the box body 10, so that the sample tube 1 can be loaded without completely opening the side door of the box body 10, reducing the exposure of the lysis assembly 22, the pipetting mechanism 50, and the opening and closing cover mechanism 30 during use, thereby effectively avoiding contamination and improving the accuracy of the subsequent nucleic acid extraction structure.

[0066] Since different object samples may use sample tubes 1 of different specifications, or different users may rotate sample tubes 1 of different models, in order to improve the versatility of the sample rack 21, in some embodiments, the sample rack 21 may also include a loading plate 214, please refer to Figure 8-Figure 10The sample plate 213 is detachably connected to the loading plate 214, the sample through hole 211 is arranged on the loading plate 214, and the sample plate 213 is provided with a through hole for exposing the sample plate 213 and the sample through hole 211. The sizes of the sample through holes 211 on different loading plates 214 can be matched with the sizes of different models of sample tubes 1, and different sample tubes 1 can be matched by replacing different loading plates 214.

[0067] In some embodiments, the installation and removal directions of the loading plate 214 and the positioning assembly 23 can be set to two opposite sides of the sample rack 21 in the vertical direction, so that the installation and removal of the loading plate 214 and the positioning assembly 23 do not affect each other.

[0068] When the drawer-type sample rack 21 is used, it is necessary to consider the positioning of the sample plate 213 after it is pushed back into place, so as to avoid movement of the sample plate 213 and the sample tube 1 during the lysis process. Specifically, any feasible positioning and limiting solution in the relevant technology can be adopted, for example, in some embodiments, a combination of an electromagnet 72 and an induction switch 74 can be used, and in other embodiments, a structure such as a ball lock 73 can be selected, which is not limited by the present invention.

[0069] In some embodiments, please refer to Figure 8 , the mounting seat 212 may include a base 215 and a U-shaped seat 216, the sample plate 213 is arranged on the opening side of the U-shaped seat 216, and is slidably matched with the two side walls of the U-shaped seat 216; the bottom surface of the U-shaped seat 216 near the base 215 has a through hole for the lysis assembly 22 to extend into. The first lifting assembly 24 includes a support plate 241, a lifting fixed plate 242 and a third driving member 243, the third driving member 243 is installed on the support plate 241, the support plate 241 is fixed on the base 215, the lifting fixed plate 242 is connected to the U-shaped seat 216, and the third driving member 243 drives the lifting fixed plate 242 to rise and fall. In order to improve the stability of lifting, in some embodiments, the first lifting assembly 24 also includes a guide rail 244, the support plate 241 and the lifting fixed plate 242 are slidably matched through the guide rail 244, and under the drive of the third driving member 243, the support plate 241 is lifted and lowered along the guide rail 244.

[0070] The third driving member 243 can be any feasible mechanism in the related art, such as an electric telescopic rod, a pneumatic telescopic rod, etc. In some embodiments, please refer to Fig.13 The third driving member 243 can be a transmission mechanism of an electric ball screw pair.

[0071] The present application does not specifically limit the specific composition and transmission method of the positioning component 23, and it can be any feasible solution in the relevant technology. For example, in some embodiments, the positioning component 23 can be a pressure plate used to press the tube cover of the sample tube 1 onto the sample plate 213, and the pressure plate is detachably fastened to the sample plate 213; in other embodiments, the positioning component 23 can also be a structural member for clamping the sample tube 1.

[0072] In some embodiments, please refer to Figure 10-12 The positioning assembly 23 may include a second driving member 231 and at least one pair of clamping members 232. The pair of clamping members 232 are arranged opposite to each other along the first direction. One end of the clamping member 232 is hinged and fixed, and the other end is transmission-connected to the output end of the second driving member 231. The second driving member 231 drives the pair of clamping members 232 to move toward and away from each other in the first direction to clamp or release the sample tube 1. By clamping the sample tube 1, the sample tube 1 can be positioned more stably.

[0073] In some embodiments, please refer to Fig.11 and Fig.12 The positioning assembly 23 also includes a mounting frame 233, and the second driving member 231 is rotatably connected to the mounting frame 233 and drives the mounting frame 233 to move in the second direction, and the second direction is perpendicular to the first direction. The mounting frame 233 is provided with a plurality of mounting holes 234 spaced apart along the first direction, and the mounting holes 234 are grouped in pairs, and a group of mounting holes 234 corresponds to a pair of clamping members 232. The second end of the clamping member 232 is installed in the mounting hole 234 to convert the displacement of the mounting frame 233 in the second direction into the displacement of the clamping member 232 in the first direction. In other words, the second end of the clamping member 232 is hingedly fixed to the sample plate 213, forming a structure with an adjustable opening at one end, so that the position of the first end can be correspondingly moved under the drive of the first driving member 31, thereby clamping or loosening the sample tube 1.

[0074] In some embodiments, in order to keep both ends of the mounting frame 233 moving synchronously and smoothly to avoid the negative effects caused by torque, please refer to Fig.12 There are two first driving members 31, and the two first driving members 31 are respectively connected to the two ends of the mounting frame 233 in the first direction.

[0075] In some embodiments, please refer to Fig.12There are two mounting frames 233, which are arranged opposite to each other in the first direction. The second driving member 231 is connected to the two mounting frames 233 in a transmission manner to drive the two mounting frames 233 to move toward and away from each other in the second direction. In the second direction, multiple pairs of clamping members 232 are arranged in two rows and are located between the two mounting frames 233. In the first direction, the clamping members 232 are opposite to each other and one end close to each other is hinged and fixed to the sample plate 213. The close ends of the two clamping members 232 located on the same side of the two pairs of clamping members 232 opposite to each other in the second direction are hinged together, and the opposite ends are respectively slidably matched with the mounting holes 234 of the two mounting frames 233, forming a structure with one end opening adjustable, and the synchronization of multiple pairs of clamping members 232 is achieved through the second driving member 231. The mounting frame 233 and the multiple pairs of clamping members 232 restrict each other, so that the operation of the positioning assembly 23 can be smoother and more stable, and the multiple pairs of clamping members 232 are concentratedly arranged between the two sample racks 21 to facilitate the subsequent operation of the cover opening and closing mechanism 30 and the pipetting mechanism 50.

[0076] Likewise, the specific selection of the second driving member 231 may be any feasible solution in the relevant technology, for example, it may be a ball screw pair, a cylinder, a hydraulic cylinder, an electrically driven telescopic rod, etc.

[0077] In some embodiments, please refer to Fig.11 and Fig.12 The second driving member 231 includes a motor 236, a rotating wheel 237 and a slide rail. The rotating wheel 237 is connected to the output end of the motor 236, and the rotating wheel 237 has a non-concentric sliding hole 239; the mounting frame 233 slides with the slide rail, and the mounting frame 233 has an acting part 235 that extends into the sliding hole 239 and cooperates with the rotating wheel 237 to form a cam-like transmission.

[0078] It should be noted that the second driving member 231 adopts a transmission mode of a motor 236, a rotating wheel 237 and a slide rail. If the number of the mounting bracket 233 is one, the number of the sliding hole 239 is correspondingly one. If the number of the mounting bracket 233 is two, in order to realize the two mounting brackets 233 moving toward each other and moving away from each other in the first direction, the number of the sliding holes 239 is also two. With the rotation of the motor 236 and the rotating wheel 237, the connection parts of the two mounting brackets 233 move along the sliding hole 239, so that the distance between the two connection parts increases or decreases.

[0079] In some embodiments, please refer to Fig.12 A bearing can be sleeved on the connecting portion of the mounting frame 233, so that the bearing can rotate and slide with the rotating wheel 237 and the sliding hole 239, thereby extending the service life.

[0080] In some embodiments, please refer to Fig. 9The sample rack 21 also includes a baffle 25 for shielding the clamping member 232 to improve the aesthetic performance and safety performance.

[0081] In some embodiments, please refer to Fig.16 The nucleic acid extraction mechanism 40 includes a slide rail, a consumable platform 42 and a nucleic acid extraction component 43. The consumable platform 42 is used to place consumables such as the reagent kit 2. The nucleic acid extraction component 43 is fixed in the box 10 and is used to complete nucleic acid washing and elution in the reagent kit 2. The slide rail is provided on the box 10, and the consumable platform 42 and the slide rail slide along the first direction to form a drawer-type platform. Along the first direction, the consumable platform 42 and the nucleic acid extraction component 43 are arranged relative to each other, and the nucleic acid extraction component 43 is located on the push-back side of the consumable platform 42. The first direction is perpendicular to the vertical direction. The nucleic acid extraction component 43 has an extraction component 431 for contacting the nucleic acid sample, and the extraction component 431 extends above the consumable platform 42. The setting of the drawer-type sample platform allows the sample platform to be pulled out of the box 10, which is convenient for manual replacement of consumables. After the box 10 is closed and the entire device is started, closed and fully automatic processing can be performed, which optimizes the replacement scheme of consumables and improves the operability of the entire device.

[0082] In some embodiments, in order to further optimize the nucleic acid sample pre-treatment device 100 and make the replacement of consumables more convenient, please refer to Figure 2 The box body 10 is provided with a second window 12 for the consumable platform 42 to be drawn out of the box body 10, and the box body 10 is also provided with a second sealing plate 14 for closing the second window 12. The second window 12 and the consumable platform 42 are directly opposite to each other in the first direction, and the consumable platform 42 can be drawn out of the box body 10 after the second sealing plate 14 is removed, so that the reagent kit 2 and other consumables can be loaded without completely opening the side door of the box body 10, reducing the exposure of the lysis component 22, the pipetting mechanism 50, and the opening and closing cover mechanism 30 during use, thereby effectively avoiding contamination, and thus improving the accuracy of the subsequent nucleic acid extraction structure.

[0083] In some embodiments, please refer to Figure 2 and Figure 3 The second cover plate 14 can be fixedly connected to the consumable platform 42, and then the second cover plate 14 and the box body 10 are positioned so that the second cover plate 14 and the box body 10 can be switched between a fixed state and a movable detachable state, for example, by a positioning pin, a rotating snap structure, etc.

[0084] In some embodiments, please refer to Fig.17In order to locate the positions of the consumable platform 42, the second sealing plate 14 and the box body 10, the nucleic acid sample pre-treatment device 100 may further include a limiting assembly 70. In some embodiments, the limiting assembly 70 may include a base 71, an electromagnet 72 and an induction switch 74. The base 71 and the consumable platform 42 are both provided with an electromagnet 72. After the consumable platform 42 is pushed back into place, the induction switch 74 is actuated, and the electromagnet 72 is energized, and the base 71 and the consumable platform 42 are adsorbed together by magnetic force. In some embodiments, the limiting assembly 70 may further include a base 71 and a ball lock 73. The ball lock 73 includes a male buckle 731 and a female buckle 732. After the consumable platform 42 is pushed back into place, the male buckle 731 and the female buckle 732 of the ball lock 73 are buckled and connected, so that the base 71 and the consumable platform 42 are connected and fixed. In some embodiments, the limit assembly 70 may also include a base 71, an electromagnet 72, an induction switch 74 and a ball lock 73. When in use, the drawer is pulled out, and consumables such as the gun tip 52 and the reagent kit 2 are placed in the corresponding area of ​​the consumable platform 42. After completion, the drawer is pushed back, and the consumable platform 42 is positioned and limited by the electromagnet 72 and the ball lock 73, and the induction switch 74 detects whether the drawer has been pushed back into place.

[0085] In some embodiments, please refer to Figure 2 and Figure 5 The sample lysis mechanism 20 and the nucleic acid extraction mechanism 40 are arranged side by side along the second direction, the second direction is the width direction of the sample lysis mechanism 20 and the width direction of the nucleic acid extraction mechanism 40, and the first direction, the second direction and the vertical direction are perpendicular to each other, that is, the first direction is the length direction of the sample lysis mechanism 20 and the length direction of the nucleic acid extraction mechanism 40. By placing the sample lysis mechanism 20 and the nucleic acid extraction mechanism 40 in the same layout orientation, the total size of the entire nucleic acid sample pretreatment device 100 in the second direction can be effectively controlled, the volume of the box body 10 can be reduced to a certain extent, and the layout of each mechanism inside the box body 10 can be made more compact, thereby shortening the travel path of the opening and closing cover mechanism 30 and the pipetting mechanism 50 to a certain extent.

[0086] In some embodiments, please refer to Figure 5 , Fig. 9 and Figure 17-Figure 18 The sample rack 21 and the consumables platform 42 are designed as pull-out structures at the same time, and are both pulled out along the first direction toward the same side of the box body 10. The user can quickly take out and put in the sample tube 1, replace the reagent kit 2 and other consumables, reducing the exposure of the lysis component 22, the opening and closing cover mechanism 30, the pipetting mechanism 50 and the extraction component.

[0087] In some embodiments, the extraction assembly includes a magnetic rod group 432, a magnetic rod set 433, a translation drive mechanism, a second lifting assembly 312 and a third lifting assembly 53. The magnetic rod group 432 and the magnetic rod set 433 together constitute the extraction component 431. The translation drive mechanism drives the magnetic rod group 432 and the magnetic rod set 433 to move back and forth synchronously in the second direction. The second lifting assembly 312 is connected to the magnetic rod group 432 to drive the magnetic rod group 432 to rise and fall. The third lifting assembly 53 is connected to the magnetic rod set 433 to drive the magnetic rod set 433 to rise and fall. The translation drive mechanism, the second lifting assembly 312 and the third lifting assembly 53 can all adopt any existing feasible solution, which is not specifically limited in this application. For example, a cylinder, a hydraulic cylinder, an electric telescopic rod or a synchronous pulley can be adopted, and for example, a combination of a motor 236, a ball screw pair and a guide rail 244 can be adopted.

[0088] It should be noted that, in addition to the reagent kit 2, the consumables required during use also include reagent bottles 5, stock solution tubes 4, multi-tubes (such as 8-tubes), etc., to assist in washing and eluting nucleic acids.

[0089] In some embodiments, please refer to Fig.17 and Fig.18 , the reagent box 2 has multiple reagent tubes. In order to place and fix the reagent box 2, a positioning member 45 and a pre-tightening member 46 are provided on the top surface of the consumable platform 42; the positioning member 45 has a concave positioning surface at one end away from the top surface of the consumable platform 42, and the positioning surface is used to contact the reagent tube and pre-position multiple reagent tubes; the pre-tightening member 46 is used to fix the reagent box 2 to the base 71. The tubular outer surface of at least part of the reagent tubes of the reagent box 2 is in contact with the positioning surface, and the positioning surface limits the movement of the reagent box 2 in all directions, and then the positions of the multiple reagent tubes of the reagent box 2 are positioned by the positioning surface. At the same time, the pre-tightening member 46 acts on the reagent box 2, plays a role in limiting the position of the reagent box 2, and fixes the reagent box 2 in the placement area of ​​the consumable platform 42. Under the joint action of the positioning member 45 and the pre-tightening member 46, the reagent box 2 is stably positioned on the consumable platform 42, ensuring that the reagent box 2 will not be easily taken out during the action of nucleic acid extraction. The structure is simple, easy to use, and the reagent box 2 can be stably positioned and fixed.

[0090] The "positioning surface is used to contact the reagent tubes and pre-position multiple reagent tubes" in the above description means that the positioning surface realizes the positioning of the reagent tube 2 by contacting the tubular bottom of the reagent tube 2. The positioning surface can be in positioning contact with one of the reagent tubes, or can be in positioning contact with two or more reagent tubes, as long as the final positioning surface can limit the displacement of the reagent tube 2 in the first direction and the second direction and position the reagent tube 2.

[0091] It should be noted that, in some embodiments, the positioning surface can be a real positioning surface formed at the end of the positioning member 45, and in other embodiments, the positioning surface can also be a virtual positioning surface defined by some structures. For example, in some embodiments, a virtual positioning surface can be defined by multiple protrusion structures arranged at the end of the positioning member 45, and each protrusion contacts different parts of the multiple reagent tubes of the reagent kit 2, thereby positioning and fixing the reagent kit 2 at multiple points.

[0092] In some embodiments, the positioning member 45 has a plurality of positioning surfaces, and the plurality of positioning surfaces are in one-to-one contact with a corresponding number of reagent tubes. That is, the number of positioning surfaces does not exceed the number of reagent tubes, and one positioning surface corresponds to the position of one reagent tube. By positioning a plurality of reagent tubes at the same time, the positioning accuracy of the positioning member 45 for the reagent box 2 can be effectively improved.

[0093] Since the bottom of a single reagent tube is hemispherical, the positioning surface can be designed to match the bottom of the hemispherical reagent tube and any solution that can position the spherical surface. For example, in some embodiments, the positioning surface can be a spherical arc surface that matches the outer surface of the reagent tube, and the depth of the depression of the spherical arc surface of the positioning surface can be less than or equal to the depth of the hemispherical bottom of the reagent tube, as long as it can meet a good positioning contact effect. Alternatively, in some embodiments, the positioning surface can also be an arc surface that matches the outer surface size of the reagent tube, and at least two of the generatrixes of the positioning surfaces are perpendicular to each other, and at least two of the generatrixes of the positioning surfaces are perpendicular to each other, then the positions of multiple reagent tubes in the plane formed by the first direction and the second direction can be determined, and multiple arc surfaces correspond to different reagent tubes respectively, thereby positioning the reagent kit 2 at multiple points.

[0094] In the nucleic acid extraction step, the reagent kit 2 needs to be heated. In some embodiments, a heating function can be integrated on the positioning member 45. For example, please refer to Fig.17 and Fig.18 In some embodiments, there are multiple positioning members 45, and the nucleic acid extraction mechanism 40 also includes a heating component, which is heat-transferably connected to at least one of the positioning members 45. Multiple positioning members 45 all have positioning surfaces that are in contact with the reagent tubes. The positioning members 45 not only play the role of positioning the reagent kit 2, but are also used to transfer the heat generated by the heating component to the reagent kit 2.

[0095] In some embodiments, a stopper 47 is also provided on the consumable platform 42 to define a placement space of a certain depth, so that the user can intuitively determine the placement position of the reagent box 2 through the stopper 47, so that the user can quickly align the positioning member 45 with the reagent tube of the reagent box 2. At least two pre-tightening members 46 are installed on the consumable platform 42 and / or the stopper 47 of the base 71, as long as they can act on the reagent box 2 to fix it. In some embodiments, the stopper 47 may include two U-shaped sub-stoppers 47, and the two U-shaped sub-stoppers 47 are detachably arranged on the bottom plate.

[0096] The present invention does not specifically limit the selection of the pre-tightening member 46. In some embodiments, the pre-tightening member 46 may be a manually adjustable structure, such as a screw for abutting the circumferential side of the reagent box 2. By manually turning the screw so that the screw abuts the reagent box 2, or making the screw not contact the reagent box 2, the reagent box 2 can be taken out and fixed. For example, in some other embodiments, the pre-tightening member 46 may be an elastic and retractable member. During the placement of the reagent box 2, the pre-tightening member 46 is abutted to compress the pre-tightening member 46, and the position of the reagent box 2 is limited by elastic force, effectively preventing the position of the reagent box 2 from changing. In addition, the pre-tightening member 46 can adapt to different reagent boxes 2 within a certain size range through adaptive expansion and contraction.

[0097] In some embodiments, please refer to Fig.17 and Fig.18 The preload member 46 is a ball screw, which can elastically contract along the first direction; there are four ball screws, which are arranged on the stopper 47 in pairs along the first direction.

[0098] In some embodiments, in order to make the distribution of the mechanisms in the box 10 more reasonable and compact, and further reduce the influence, interference and pollution between the mechanisms, please refer to Figure 4The box body 10 has a middle partition 15, which is Z-shaped and includes a first plate 151, a second plate 152, and a third plate 153 connecting the first plate 151 and the second plate 152. The first plate 151 and the second plate 152 are arranged at intervals in the vertical direction. The middle partition 15 divides the box body 10 into a first layer 16 and a second layer 17 which are arranged in sequence from bottom to top in the vertical direction; the sample lysis mechanism 20 and the nucleic acid extraction mechanism 40 are arranged on the first layer 16, the liquid transfer mechanism 50 and the cover opening and closing mechanism 30 are arranged on the second layer 17, and a first connecting window 154 exposing the sample tube 1 is opened on the first plate 151. and a second connecting window 155 exposing the consumable platform 42, the consumable platform 42 is located between the first plate 151 and the box body 10, the nucleic acid extraction component 43 is arranged between the second plate 152 and the box body 10, the third plate 153 is provided with a third connecting window 156, so that the extraction component 431 can extend into and move above the consumable platform 42, the opening and closing cover mechanism 30 contacts the sample tube 1 through the first connecting window 154, and the pipetting mechanism 50 absorbs the sample in the sample tube 1 through the first connecting window 154 and the second connecting window 155 and moves to the nucleic acid extraction mechanism 40 to discharge the sample into the reagent kit 2.

[0099] In some embodiments, in order to further reduce the mutual influence between the nucleic acid extraction mechanism 40 and the sample lysis mechanism 20 arranged on the first layer 16, please refer to Figure 3 and Figure 4 A first isolation plate 18 is also provided in the first layer 16. Along the second direction, the first isolation plate 18 is provided between the nucleic acid extraction mechanism 40 and the sample lysis mechanism 20. The middle partition 15 extends from the area corresponding to the extraction component to the first layer 16 to the box body 10 to form an extended partition to separate the consumable platform 42 and the extraction component. A hole for wiring can be opened on the extended partition according to actual needs, which is not limited in this application.

[0100] In some embodiments, please refer to Figure 4 and Fig.16 In order to further separate the consumable platform 42 and the extraction component, the extraction component also includes a second partition for the magnetic rod group 432 and the magnetic rod set 433 to pass through. The second partition and the middle partition 15 cooperate to close the third connecting window 156 without affecting the translation and lifting movements of the extraction component 431.

[0101] In some embodiments, the liquid transfer mechanism 50 and the cover opening and closing mechanism 30 can be driven to translate by corresponding drive mechanisms respectively. Fig.14 and Fig.15, the nucleic acid sample pre-treatment device 100 also includes a moving arm 60, and the pipetting wall is arranged on the box body 10 and is located on the second layer 17; the pipetting mechanism 50 and the cover opening and closing mechanism 30 are driven by the moving arm 60 to move in a plane perpendicular to the vertical direction, which can simplify the structure, omit some driving actuators, and can effectively control the volume of the second layer 17 and the box body 10, making the entire module more refined. Since the pipetting mechanism 50 and the cover opening and closing mechanism 30 both need to move in the vertical direction and need to avoid mutual interference, the first driving member 31 includes a rotating component 311 that drives the clamp to rotate and a second lifting component 312 that drives the clamp to rise and fall in the vertical direction; the pipetting mechanism 50 also includes a third lifting component 53 that drives the sampling gun 51 to rise and fall in the vertical direction, and the second lifting component 312 and the third lifting component 53 are driven separately, so that the vertical movements of the pipetting mechanism 50 and the cover opening and closing mechanism 30 can be independent of each other and do not interfere with each other. The sampling gun 51 can select an ADP pipette or any feasible structure or module device, which is not limited in this application.

[0102] In some embodiments, in order to reduce contamination between multiple samples and improve the efficiency of pipetting multiple sample tubes 1, a placement portion 421 for placing multiple gun tips 52 is provided in the box 10; the sampling gun 51 has a connecting structure for installing the gun tip 52, and the sampling gun 51 and the gun tip 52 are detachably connected through the connecting structure. The sampling gun 51 moves to the placement portion 421 to replace the gun tip 52. At this time, the pipetting mechanism 50 does not need to have the function of washing and rinsing. After the pipetting of a sample tube 1 is completed, it automatically reaches the placement portion 421 to replace the gun tip 52, which can further improve the accuracy and reliability of the test results.

[0103] In some embodiments, the gun tip 52 is used as a consumable, and the placement portion 421 can be optionally disposed on the consumable platform 42 so that various consumables can be placed centrally, which is also convenient for loading and unloading.

[0104] The following is a specific example to illustrate the usage of each mechanism of the nucleic acid sample pretreatment device 100.

[0105] 1. Experimental preparation:

[0106] ① Pull out the sample plate 213 through the first window 11, put the sample tubes 1 into the sample through holes 211 of the sample plate 213 one by one, and push the sample plate 213 back to the working position.

[0107] ② Pull out the consumables platform 42 through the second window 12, and put the prepared 50ul gun tip 52, 1000ul gun tip 52, reagent kit 2, eight-tube 3, reagent bottle 5 and stock solution tube 4 into the corresponding area of ​​the consumables drawer mechanism. Place the gun tip 52, reagent kit 2 and other consumables required in the corresponding positions, and push the consumables platform 42 back into place.

[0108] 2. Select the corresponding experimental program on the interface and start the experimental steps. The instrument begins the entire experimental process and all mechanisms are reset once before starting operation.

[0109] 3. Sample lysis: The positioning assembly 23 is actuated to clamp the sample tube 1, and then the first lifting assembly 24 drives the sample rack 21, the sample tube 1 and the positioning assembly 23 to move downward along the Z axis. When the sample tube 1 descends to a certain depth in the lysis chamber 223 of the lysis platform 221, it stops, and then the lysis assembly 22 performs ultrasonic lysis on the sample. After completion, the first lifting assembly 24 drives the sample rack 21, the sample tube 1 and the positioning assembly 23 to move upward along the Z axis and return to their original positions.

[0110] 4. Opening the cover of sample tube 1: the movable arm 60 drives the cover opening and closing mechanism 30 and the pipetting mechanism 50 to move as a whole to the top of the test tube rack, and then the cover opening member 32 (claw) of the second lifting assembly 312 descends to the position of sample tube 1, and the claw clamps the tube cover. While the rotating assembly 311 drives the claw to rotate, the second lifting assembly 312 moves upward as a whole, driving the claw to complete the opening of a sample tube 1.

[0111] 5. Sample transfer: After the cover is opened, the movable arm 60 drives the sampling gun 51 to move to the sample tube 1, and the third lifting assembly 53 drives the sampling gun 51 to move to the sample tube 1 to absorb the sample, and under the joint drive of the third lifting assembly 53 and the movable arm 60, the sampling gun 51 moves to the position of the reagent kit 2 to discharge the sample into the reagent tube corresponding to the reagent kit 2.

[0112] 6. Nucleic acid extraction: The magnetic rod is initially inserted into the magnetic rod sleeve and fixed in place. Under the joint action of the translation drive assembly 434, the fourth lifting assembly 435 and the fifth lifting assembly 436, the samples are transferred one by one in the reagent kit 2, and washed and eluted at each position to finally complete the nucleic acid extraction.

[0113] 7. System construction: The mobile arm 60 drives the pipetting mechanism 50 to dispense the reaction solution into 8 connected tubes in turn, and then the 8 groups of extracted nucleic acids are added into the 8 connected tubes respectively to complete the system construction.

[0114] The above specific examples are used to illustrate the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the art, according to the concept of the present invention, some simple deductions, modifications or substitutions can be made.

Claims

1. A nucleic acid sample pretreatment device, characterized in that: include: Box; The sample lysis mechanism comprises a sample rack and a lysis assembly; the sample rack has a plurality of sample through holes for placing a plurality of sample tubes; The lysis assembly has a lysis station for performing cell nucleus lysis on a sample in a sample tube extending into the lysis station; The cover opening and closing mechanism comprises a first driving member and a cover opening member, wherein after the cell nucleus lysis process, the first driving member drives the cover opening member to open the tube cover of the sample tube; A nucleic acid extraction mechanism, used for performing nucleic acid extraction operations on the lysed nucleic acid sample; and a liquid transfer mechanism, including a movable sampling gun, wherein the sampling gun equipped with a gun head moves to the opened sample tube to extract the sample, and moves to the nucleic acid extraction mechanism to discharge the sample; The sample lysis mechanism, the cover opening and closing mechanism, the nucleic acid extraction mechanism and the liquid transfer mechanism are all arranged in the box.

2. The nucleic acid sample pretreatment device according to claim 1, characterized in that: The sample rack is arranged above the lysis component, and the plurality of sample through holes are projected into the lysis station; the sample lysis mechanism further comprises a positioning component installed on the sample rack to fix the plurality of sample tubes; The sample lysis mechanism also includes a first lifting component, which is transmission-connected to the sample rack or the lysis component so that the sample rack and the lysis component are moved closer to or farther away from each other in the vertical direction, so that the sample tube can be extended into the lysis station or be separated from the lysis station.

3. The nucleic acid sample pretreatment device according to claim 2, characterized in that: The sample rack includes a mounting seat and a sample plate, and the mounting seat and the sample plate are slidably matched in a first direction to form a drawer-type sample rack; the first direction is perpendicular to the vertical direction, and the sample rack is fixedly installed in the box body through the mounting seat, the positioning assembly is installed on the sample plate, and the sample through hole is located on the sample plate, and the first lifting assembly is transmission-connected to the mounting seat to drive the mounting seat, the sample plate and the positioning assembly to rise and fall as a whole along the vertical direction.

4. The nucleic acid sample pretreatment device according to claim 3, characterized in that: The box body is provided with a first window for the sample plate and the positioning assembly to be drawn out of the box body along the first direction, and the box body also has a first sealing plate for closing the first window.

5. The nucleic acid sample pretreatment device according to any one of claims 1 to 4, characterized in that: The nucleic acid extraction mechanism comprises a slide rail, a consumables platform and a nucleic acid extraction component, wherein the consumables platform is used to place consumables such as a reagent kit, and the nucleic acid extraction component is used to complete nucleic acid washing and elution in the reagent kit; The slide rail is arranged on the box body, and the consumables platform and the slide rail slide together along a first direction to form a drawer-type platform; Along the first direction, the consumable platform and the nucleic acid extraction component are arranged opposite to each other, and the nucleic acid extraction component is located on the push-back side of the consumable platform. The first direction is perpendicular to the vertical direction. The nucleic acid extraction component has an extraction component for contacting the nucleic acid sample, and the extraction component extends to the top of the consumable platform.

6. The nucleic acid sample pretreatment device according to claim 5, characterized in that: The sample lysis mechanism and the nucleic acid extraction mechanism are arranged side by side along a second direction, the second direction is a width direction of the sample lysis mechanism and a width direction of the nucleic acid extraction mechanism, and the first direction, the second direction and the vertical direction are perpendicular to each other.

7. The nucleic acid sample pretreatment device according to claim 5, characterized in that: The box body has a middle partition, which is Z-shaped and includes a first plate, a second plate and a third plate connecting the first plate and the second plate. The first plate and the second plate are arranged at intervals in the vertical direction, and the middle partition divides the box body into a first layer and a second layer distributed in sequence from bottom to top in the vertical direction; the sample lysis mechanism and the nucleic acid extraction mechanism are arranged on the first layer, the pipetting mechanism and the opening and closing cover mechanism are arranged on the second layer, the first plate is provided with a first connecting window exposing the sample tube and a second connecting window exposing the consumable platform, the consumable platform is located between the first plate and the box body, the nucleic acid extraction component is arranged between the second plate and the box body, and the third plate is provided with a third connecting window for the extraction component to extend above the consumable platform and move.

8. The nucleic acid sample pretreatment device according to claim 5, characterized in that: The box body is provided with a second window for the consumables platform to be drawn out of the box body, and the box body also has a second sealing plate for closing the second window.

9. The nucleic acid sample pretreatment device according to claim 7, characterized in that: The nucleic acid sample pretreatment device also includes a moving arm, and the pipetting wall is arranged on the box body and is located on the second layer; the pipetting mechanism and the opening and closing cover mechanism are driven by the moving arm to move in a plane perpendicular to the vertical direction; the first driving member includes a rotating component that drives the clamp to rotate and a second lifting component that drives the clamp to rise and fall in the vertical direction; the pipetting mechanism also includes a third lifting component that drives the sampling gun to rise and fall in the vertical direction.

10. The nucleic acid sample pretreatment device according to any one of claims 1 to 4, characterized in that: The box body is provided with a placement portion for placing multiple gun heads; the sampling gun has a connection structure for installing the gun head, the sampling gun and the gun head are detachably connected through the connection structure, and the sampling gun is moved to the placement portion to replace the gun head.

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