Growth-promoting stress-resistant seed coating agent containing pollen polysaccharide biostimulant and preparation method thereof

Abstract

The invention discloses a growth-promoting stress-resistant seed coating agent containing pollen polysaccharide biostimulant and a preparation method thereof, and relates to the technical field of seed coating agents. The invention provides a preparation method and a use method of a seed coating agent. The seed coating agent containing the pollen polysaccharide is prepared for the first time, the emergence rate of seeds using the seed coating agent containing the pollen polysaccharide is obviously increased after sowing, and grown plants have obvious lodging resistance and stress resistance.

Description

Technical Field

[0001] The priority information of the present invention is as follows: priority number is 202311259550.0, priority date is September 27, 2023, the name of the invention is a growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant and a preparation method thereof, and the disclosure of which is incorporated herein by reference. The present invention relates to the technical field of seed coating agents, and in particular to a growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant and a preparation method thereof. Background Art

[0002] In agricultural production, growth promoters are sometimes needed to promote plant growth, including promoting the growth of plant roots and promoting the growth of the above-ground stems and leaves of plants. At present, the most widely used safe growth promoter on the market is brassinolide (BR). Currently, there are two main sources of brassinolide: natural extraction and artificial synthesis.

[0003] During the extraction of natural brassinolide, a large amount of pollen polysaccharides will be produced. The patent document with application number 202010065033.X discloses pollen polysaccharide extract and its application in promoting plant growth. It is found that pollen polysaccharides, a byproduct produced during the extraction of natural brassinolide, have a good promoting effect on the growth of crops. Through foliar spraying or root irrigation, it has a good promoting effect on the roots, stems, leaves, etc. of crops within a certain concentration range. The components of pollen polysaccharides were tested and it was found that pollen polysaccharides contain multiple components such as sugars, proteins, amino acids and lipids.

[0004] At present, most of the research on pollen polysaccharides is in the fields of food and medicine, and in agriculture it is limited to promoting the growth of plant roots and the growth of the above-ground parts of plants such as stems and leaves, and there is no related application in seed dressing agents. Summary of the invention

[0005] The purpose of the present invention is to provide a growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant and a preparation method thereof, to develop a seed coating agent formula with pollen polysaccharide added, and to use the formula to prepare a seed coating agent that can promote seed germination and improve the stress and lodging resistance of plants during growth, thereby extending the application of pollen polysaccharides to a wider field.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant comprises the following components by weight:

[0008] Pollen polysaccharide extract 30-50%, film former 0.5-4%, antifreeze 3-5%, plant growth regulator 0.05-0.2%, fungicide 1-8%, insecticide 2-6%, colorant 0.01-0.2%, trace elements 1.1-2.4%, and the balance is water.

[0009] The extraction method of the pollen polysaccharide extract includes, but is not limited to, water extraction and alcohol precipitation, chitosan precipitation and other conventional methods.

[0010] The chitosan precipitation method comprises the following steps: mixing rapeseed pollen and water, heating and stirring to extract at least once, filtering, combining the filtrate, adding chitosan to the filtrate to obtain a clarified liquid, maintaining the clarified liquid at 60-80° C. for at least 1 hour, cooling and standing, separating the solid from the liquid, and obtaining the liquid as the pollen polysaccharide extract.

[0011] Preferably, the extraction method of the pollen polysaccharide extract is as follows: rapeseed pollen and water are mixed in a weight ratio of 1:2, heated to 65°C, stirred for 4 hours, and a stirring rate of 3500r / min, extracted twice, filtered, and the filtrate is combined, chitosan is added to the filtrate to obtain a clarified liquid, the clarified liquid is maintained at 70°C for 1 hour, cooled and allowed to stand, and then the solid-liquid separation is performed, and the obtained liquid is the pollen polysaccharide extract.

[0012] Furthermore, the film-forming agent is selected from any one or more of sodium alginate, chitosan, and sodium carboxymethyl cellulose.

[0013] Furthermore, the antifreeze agent is selected from one or more of ethylene glycol, propylene glycol and urea.

[0014] Furthermore, the plant growth regulator is selected from one or more of gibberellins and triacontanol.

[0015] Furthermore, the fungicide is selected from any one or more of carbendazim, difenoconazole and meconazole.

[0016] Furthermore, the insecticide is selected from any one or more of imidacloprid, acetamiprid and thiamethoxam.

[0017] Furthermore, the colorant is selected from one or more of the dye GRB and alkaline rose essence.

[0018] Furthermore, the trace element is selected from one or more of boron fertilizer, manganese fertilizer and zinc fertilizer;

[0019] Furthermore, the boron fertilizer is selected from one or more of boric acid, sodium tetraborate, and borax, the manganese fertilizer is selected from one or more of manganese sulfate, manganese chloride, and EDTA-Mn, and the zinc fertilizer is selected from one or more of zinc sulfate, zinc chloride, and EDTA-Zn.

[0020] The preparation method of the seed coating agent comprises the following steps:

[0021] 1) Weigh a certain amount of water in advance, add the pollen polysaccharide extract, stir evenly to make a solution;

[0022] 2) Raise the temperature to 70-80° C., weigh the film-forming agent according to the mass percentage of the above formula, add it to the above solution, stir while adding, until the film-forming agent is completely dissolved, to obtain a composite film-forming agent solution;

[0023] 3) Weigh antifreeze agent, plant growth regulator, fungicide, insecticide, colorant, and trace elements according to the mass percentage of the above formula composition, add them to the above composite film-forming agent solution, mix them evenly, and obtain the target seed coating agent. The method of using the seed coating agent is to coat seeds with the seed coating agent containing pollen polysaccharide, and the specific method is as follows:

[0024] 2-3 days before sowing, add the seed coating agent to the seeds, stir evenly, and dry them in a natural state;

[0025] In one aspect, the ratio of the seed coating agent to the seeds is 200-350 ml / 100 kg. In another aspect, the ratio of the seed coating agent to the seeds is 100 ml / 100 kg-400 ml / 100 kg.

[0026] The seed coating agent has the application of improving the growth-promoting and stress-resistance performance of plants under adverse environments, wherein the adverse environments include: one or more of saline-alkali, drought, and low temperature.

[0027] Dye GRB: Dark acid scarlet GR, used for dyeing wool and silk fabrics, paper and leather. It can also be used for coloring plastics, wood, spices, medicine, electroplated aluminum and cement. It can also be used to make ink. Its heavy metal salts can be used as pigments.

[0028] EDTA-Zn: Ethylenediaminetetraacetic acid sodium zinc, the molecular formula is EDTAZnNa 2 ;

[0029] EDTA-Mn: Ethylenediaminetetraacetic acid manganese sodium, the molecular formula is EDTA.MnNa 2 .

[0030] The beneficial effects of the present invention are:

[0031] 1. The present invention prepares a seed coating agent containing pollen polysaccharides for the first time. The germination rate of seeds coated with the seed coating agent containing pollen polysaccharides is significantly improved after sowing, and the grown plants have obvious lodging resistance, stress resistance and cold resistance.

[0032] 2. Compared with the seed coating agent with the same formula but without pollen polysaccharide, the seed coating agent containing pollen polysaccharide prepared by the present invention has improved adhesion, reduced volume of separated matter and increased stability after cold storage.

[0033] 3. The seed coating agent of the present invention is applied to saline-alkali land.

[0034] 4. The seed coating agent containing pollen polysaccharide in an amount of 100ml / 100kg to 400ml / 100kg of the present invention is used for seed treatment and germination of crops such as corn, wheat, cotton, and soybean. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 : Plant status of coated and uncoated corn seedlings after 4 days of drought stress. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is described clearly and completely below. Obviously, the embodiments described here are only a part of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the protection scope of the present invention.

[0037] Example 1

[0038] A method for preparing a growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant, the specific steps are as follows:

[0039] 1) Weigh 553.2 g of water in advance, add 300 g of pollen polysaccharide extract, stir evenly to make a solution;

[0040] 2) Raise the temperature to 70°C, weigh 15 g of chitosan and add it to the above solution, stirring until the chitosan is completely dissolved, to obtain a composite film-forming agent solution;

[0041] 3) Weigh 30 g of urea, 0.5 g of gibberellin, 50 g of polybrene, 40 g of acetamiprid, 0.3 g of dye GRB, 4 g of EDTA-Zn, 3 g of EDTA-Mn, and 4 g of boric acid, add them to the above composite film-forming agent solution, mix well, and obtain No. 1 seed coating agent.

[0042] The addition amount of each component in the seed coating agent formula was adjusted, and the temperature of the film-forming reaction was changed to obtain seed coating agents No. 2-14 containing pollen polysaccharides. The formula parameters (by weight percentage) and temperature parameters of seed coating agents No. 1-7 and No. 8-14 are shown in Table 1 and Table 2, respectively. The cold and hot storage stability and formulation appearance of seed coating agents No. 1-14 were judged, as shown in Table 3.

[0043] Table 1 Formula parameters and temperature parameters of seed coating agents No. 1 to No. 7

[0044]

[0045]

[0046] Table 2 Formula parameters and temperature parameters of seed coating agents No. 8-14

[0047]

[0048] Table 3 Preparation parameters of seed coating agents No. 1 to No. 14

[0049]

[0050]

[0051] By comparing seed coating agents No. 1 to No. 14, the preferred small test samples with a viscosity between 250-270mPa·s, uniformity ≥ 95%, detachment rate ≤ 0.6%, uniform appearance, and no stratification, precipitation, crystallization, etc. during hot and cold storage were used for effect verification. The small test formulas that met the conditions were No. 4, No. 10, No. 11, and No. 12, which were selected as the preferred seed coating formulas.

[0052] Meanwhile, it can be seen from Tables 1 to 3 that the pH value of the seed coating agent of the present invention does not exceed 6.8, and is preferably 6.3-6.8. At this pH value, it is ensured that the seed coating agent can be used on saline-alkali land.

[0053] Meanwhile, it can be seen from Tables 1 to 3 that the seed coating agent of the present invention can be stored at -18 to 54° C. For example, in the northwest region, especially in Qinghai, Gansu, Xinjiang and other regions, the climate conditions in the region are harsh, and a lot of land is saline-alkali land, which is not convenient for seed germination, and the seed coating agent of the present invention can be used in this region.

[0054] Example 2

[0055] Effects of using No. 4, No. 10, No. 11, and No. 12 seed coating agents on corn germination:

[0056] Treat with seed dressing agents No. 4, 10, 11 and 12 in Example 1, and the dosage of the seed dressing agent is 150ml / 100kg. Corn seeds (Zhengdan 958) are diluted according to the drug slurry and seed mass ratio of 1:50 (i.e., 100 kg of seeds plus 2L of water), and are fully stirred with the corn seeds until the drug solution is evenly distributed on the surface of the seeds, and then dried. Take 150ml / 100kg of Si Le Shi (common seed dressing agent, recommended dosage for corn) as the positive control group; seeds treated with clean water are used as the blank control group. The germination rate, plant height and root length of corn are investigated. Vermiculite and coconut bran after foaming were mixed evenly in a volume ratio of 3:1, and placed in a 20cm×10cm×10cm square culture box. 300g of culture matrix was added to each culture box, and 180g of water was added. The matrix was stirred evenly, and 20 holes of the same depth were evenly pressed using a sowing machine. The coated seeds were sown with tweezers, 20 seeds per pot, and 3 replicates were used for each treatment. Then, the soil was lightly covered, the lid was covered to keep moisture, and the culture was placed in the culture room for cultivation at a temperature of 26℃. The light cycle was 10h / 14h (day / night), and the evaporated water of each treatment was supplemented after weighing every day to keep the water supply consistent during the treatment.

[0057] Seven days after sowing, the germination rate, corn plant height and root length of each treatment were investigated.

[0058] Germination rate (%) = (total number of germinated seeds / total number of tested seeds) × 100; corn plant height (the distance from the base of the plant to the tip of the uppermost expanded leaf); root length (the distance from the base of the plant to the tip of the longest root underground).

[0059] The seeds treated with Celexa were used as the first control group, and the seeds treated with water were used as the second control group. After 2 weeks, the germination rate, plant height and root length of corn were investigated, and the results are shown in Table 4.

[0060] Table 4 Effects of different seed coating agents on corn germination

[0061]

[0062]

[0063] As shown in Table 4, the germination rate of corn seeds treated with pollen polysaccharide seed coating agents No. 4, No. 10 and No. 11 was higher than that treated with Seed Serase, among which the plant height and root length of corn treated with Seed Serase No. 4 were better than those of Seed Serase and blank control, showing the best effect.

[0064] Example 3

[0065] The invention discloses an application of seed coating agent containing pollen polysaccharide to corn germination by coating with different dosages.

[0066] In the preferred embodiment 1, the No. 4 seed coating agent is used as a treatment, and corn seeds are coated with different seed coating agent dosages of 50ml / 100kg, 100ml / 100kg, 150ml / 100kg, 200ml / 100kg, and 250ml / 100kg, and the No. 4 seed coating agent without pollen polysaccharide is used as a preparation control, and clear water is used as a blank control. Corn seeds (Zhengdan 958) are diluted with different dosages of the preparation agent according to the mass ratio of the slurry to the seeds of 1:50 (i.e., 100 kg of seeds plus 2L of water), and fully stirred with the corn seeds until the liquid is evenly distributed on the surface of the seeds, and then dried.

[0067] Vermiculite and coconut bran after foaming were mixed evenly in a volume ratio of 3:1, and placed in a 20cm×10cm×10cm square culture box. 300g of culture matrix was added to each culture box, and 180g of water was added. The matrix was stirred evenly, and 20 holes of the same depth were evenly pressed using a sowing machine. The coated seeds were sown with tweezers, 20 seeds per pot of soil, and 3 replicates were applied for each treatment. Then the soil was lightly covered, the lid was covered to keep moisture, and the culture was placed in the culture room for cultivation at a temperature of 26℃. The light cycle was 10h / 14h (day / night), and the evaporated water of each treatment was supplemented after weighing every day to keep the water supply consistent during the treatment period. The germination rate of each treatment was investigated after 10 days, as shown in Table 5.

[0068] Table 5 Effect of seed coating dosage on corn seed germination

[0069]

[0070] It can be seen from Table 5 that when the No. 4 seed coating agent is used at a drug-seed ratio of 200 ml / 100 kg and above, the seed germination rate is high, and there is no change in the seed germination rate when it exceeds 200 ml / kg. Under the same dosage, adding pollen polysaccharides can increase the germination rate of corn seeds. The optimal dosage for corn coating is 200 ml / kg.

[0071] Example 4

[0072] The No. 4 pollen polysaccharide seed coating agent in Example 1 was used as the treatment, and the dosage of the seed coating agent preparation was 200ml / 100kg. Corn seeds (Zhengdan 958) were diluted according to the drug slurry and seed mass ratio of 1:50 (that is, 100 kg of seeds plus 2L of water), and the corn seeds were fully stirred until the drug solution was evenly distributed on the surface of the seeds, and then dried. Uncoated seeds were used as the control, and the seeds were sown in the matrix (vermiculite: coconut bran volume ratio = 3:1), placed in the culture room for cultivation, and the temperature was set at 26°C. The photoperiod was 10h / 14h (day / night), and the water supply was kept consistent during the seedling period.

[0073] When the corn seeds grow to the two-leaf stage (about 14 days), select corn seedlings with consistent growth and irrigate with 18% (W / V) PEG6000 nutrient solution for drought stress. Each treatment has 12 seedlings as one replicate, and each treatment has 3 replicates. The changes of leaves are observed under drought stress. After 3 days of stress treatment, the dry weight, fresh weight and water content of leaves of seedlings in each treatment under PEG6000 stress treatment are measured.

[0074] The leaf moisture content (%) = (leaf fresh weight - leaf dry weight) / leaf fresh weight × 100.

[0075] The results are shown in Table 6.

[0076] Table 6 Effects of seed coating on drought resistance of corn seedlings

[0077] deal with Leaf water holding rate1 Leaf water retention 2 Leaf water retention 3 Average water holding capacity Coated seeds 91.36％ 90.15％ 91.79％ 91.09％a Uncoated seeds 73.47％ 77.52％ 74.61％ 75.20％b

[0078] As shown in Table 6, when the corn seeds coated with No. 4 pollen polysaccharide grow to the two-leaf stage, the water holding rate of a single leaf and the average water holding rate of the leaves are significantly higher than those of the uncoated corn seeds, indicating that the seed coating agent of the present invention can improve the drought resistance of crops.

[0079] Example 5

[0080] Effects of Seed Coating Agents on Soybean Germination under Alkaline Conditions

[0081] The No. 4 pollen polysaccharide seed coating agent in Example 1 was used as the treatment, and the preparation dosage was 200ml / 100kg, 250ml / 100kg, 300ml / kg, 350ml / kg, 400ml / kg. The soybean seeds were coated according to the drug slurry and seed mass ratio of 1:50. The seeds were fully stirred until the drug solution was evenly distributed on the seed surface and dried. The seeds treated with clean water were used as the blank control group. The No. 4 seed coating agent without pollen polysaccharide was used as the preparation control.

[0082] Use the saline-alkali soil collected from Xinjiang for sowing. The soluble salt in the soil is 960mg / kg, the EC value is 1600us / cm, and the pH is 9.23, which belongs to alkaline soil. Mix the alkaline soil, put it into the germination box, fill it with 800g of soil, and evenly and slowly add 200g of water. At this time, the alkaline soil is basically soaked. Use a seeder to evenly press out 20 holes of the same depth, and use tweezers to sow the coated seeds. There are 20 seeds per pot of soil, and 5 repetitions for each treatment. Then cover the soil lightly, cover the lid to keep it moist, and culture it in the culture room. The temperature is set at 26°C. During the germination process, pay attention to observe the soil water conditions at any time and replenish water in time. Each replenishment can be replenished to the weight at the time of sowing, that is, 1000g. Control the room temperature at 25°C, and observe the effect of the amount of seed coating agent on the germination of soybean seeds after 10 days. The results are shown in Table 7.

[0083] Table 7 Effect of seed coating agent on soybean germination under alkaline conditions

[0084]

[0085] As shown in Table 7, under alkaline soil conditions, the use of pollen polysaccharide seed coating agent to coat soybeans can increase the soybean germination rate, among which the pollen polysaccharide germination rate is the highest when the dosage is 350ml / 100kg.

[0086] Example 6

[0087] Effects of using pollen polysaccharide seed coating agent on corn germination under saline-alkali conditions

[0088] The No. 4 pollen polysaccharide seed coating agent in Example 1 was used as the treatment, the dosage of the preparation was 200 ml / 100 kg, and the corn seeds (Zhengdan 958) were coated according to the drug slurry and seed mass ratio of 1:50. The seeds were fully stirred until the drug solution was evenly distributed on the seed surface and dried. The No. 4 seed coating agent without pollen polysaccharide (supplemented with water) was used as the preparation control group; the seeds treated with clean water were used as the blank control group.

[0089] Use the saline-alkali soil collected from Xinjiang for sowing. The soluble salt in the soil is 960mg / kg, the EC value is 1600us / cm, and the pH is 9.23, which belongs to alkaline soil. Mix the alkaline soil, put it into the germination box, fill it with 800g of soil, and evenly and slowly add 200g of water. At this time, the alkaline soil is basically soaked. Use a seeder to evenly press out 20 holes of the same depth, and use tweezers to sow the coated seeds. There are 20 seeds per pot of soil, and 5 repetitions for each treatment. Then cover the soil lightly, cover the lid to keep it moist, and culture it in the culture room. The temperature is set at 26°C. During the germination process, pay attention to observe the soil water condition at any time and replenish water in time. Each replenishment can be replenished to the weight at the time of sowing, that is, 1000g (the amount of seeds is negligible). Control the room temperature at 26°C, and observe the effect of the amount of seed coating agent on the germination of corn seeds after 12 days. The results are shown in Table 8.

[0090] Table 8 Effect of seed coating agent on germination of corn under alkaline conditions

[0091] Coating treatment Germination rate (%) No. 4 pollen polysaccharide seed coating agent 66.67a Seed coating agent No. 4 (without pollen polysaccharide) 33.33b Shimizu 36.66b

[0092] It can be seen from Table 8 that under alkaline soil conditions, using 200ml / 100kg of seed coating agent containing pollen polysaccharides to coat corn can increase the germination rate of corn under alkaline conditions.

[0093] Example 7

[0094] Effects of Seed Coatings Containing Pollen Polysaccharides on Wheat Germination under Normal Conditions

[0095] The pollen polysaccharide seed coating agent No. 4 in Example 1 was used as a treatment, and wheat seeds were coated with different seed coating agent preparation dosages of 50ml / 100kg, 100ml / 100kg, 200ml / 100kg, and 300ml / 100kg. At the same time, the seed coating agent No. 4 without adding pollen polysaccharides was used for comparison, with a dosage of 100ml / 100kg. The wheat seeds (Jimai 22) were diluted with different dosages of the preparation according to a slurry to seed mass ratio of 1:50 (i.e., 100 kg of seeds plus 2L of water), and then fully stirred and mixed with the wheat seeds until the liquid was evenly distributed on the seed surface and dried.

[0096] Vermiculite and expanded coconut bran were mixed with water in a volume ratio of 3:1, and the same weight of the matrix was weighed and placed in a flower pot. Wheat seeds were sown into the matrix, and water was added during the culture period. The quality of wheat seedlings was investigated after 15 days of culture. The results are shown in Table 9.

[0097] Table 9 Effects of seed coating on wheat seedlings

[0098]

[0099]

[0100] As shown in Table 9, under the tested concentrations, the germination rate of 100ml / 100kg was the highest. All growth indicators of wheat treated with seed coating agent at the test dose of 100ml / 100kg were significantly better than those treated with clean water, and it had a significant promoting effect on the growth of wheat seedlings.

[0101] Example 8

[0102] Effects of seed coating containing pollen polysaccharides on wheat germination under drought conditions

[0103] The pollen polysaccharide seed coating agent No. 4 in Example 1 was used as the treatment, and the seed coating agent No. 4 without pollen polysaccharide extract (supplemented with water) was used as the control. The wheat seeds were coated with 100 ml of the drug for 100 kg of seeds. The wheat seeds (Jimai 22) were diluted with different amounts of the preparation according to the mass ratio of the drug slurry to the seeds of 1:50 (i.e., 100 kg of seeds plus 2 L of water), and then fully stirred and mixed with the wheat seeds until the drug solution was evenly distributed on the seed surface. After drying, the experimental treatment was carried out.

[0104] Vermiculite and expanded coconut bran were mixed evenly with 20% PEG6000 aqueous solution in a volume ratio of 3:1, and the same weight of the matrix was weighed and placed in a flower pot. Wheat seeds were sown into the matrix, and the quality of the wheat seedlings was investigated after 8 days of cultivation. The results are shown in Table 10 below.

[0105] Table 10 Effects of pollen polysaccharide seed coating on wheat germination and seedling growth under drought conditions

[0106] Coating treatment Root length (cm) Bud length (cm) Germination rate (%) No. 4 pollen polysaccharide seed coating agent (drought) 8.54b 4.21b 73.33％b Seed coating agent No. 4 (without pollen polysaccharide and drought) 6.54c 3.21c 52.33％c No coating (dry) 7.05bc 3.42bc 59.00％c No coating (no drought) 13.4a 12.8a 93.00％a

[0107] It can be seen from Table 10 that adding pollen polysaccharides to the seed coating agent can effectively alleviate the inhibitory effect of drought stress on wheat germination and growth, increase the germination rate, and promote wheat growth.

[0108] Example 9

[0109] Effects of seed coating agents containing pollen polysaccharides on cotton germination under saline-alkali stress

[0110] The pollen polysaccharide seed coating agent No. 4 in Example 1 was used as the treatment, and the seed coating agent No. 4 (supplemented with water) without pollen polysaccharide extract was used as the control. Cotton seeds were coated with 100 ml of the drug for 100 kg of seeds. The preparations with different dosages were diluted according to the mass ratio of the drug slurry to the seeds of 1:50 (i.e., 100 kg of seeds plus 2 L of water), and then fully stirred and mixed with the cotton seeds until the drug solution was evenly distributed on the seed surface. After drying, the test treatment was carried out.

[0111] Add vermiculite and expanded coconut husk in a volume ratio of 3:1 and add saline-alkali concentration (mmol / L) of NaCl:Na 2 SO 4 :NaHCO 3 :Na 2 CO 3 =9:1:9:1 saline-alkali aqueous solution was mixed evenly, the same weight of substrate was weighed and put into the flower pot, cotton seeds were sown into the substrate, and water was added quantitatively during the culture period. The quality of cotton seedlings was investigated after 8 days of culture. The results are shown in Table 11 below.

[0112] Table 11 Effects of pollen polysaccharide seed coating on cotton germination and seedling growth under saline-alkali conditions

[0113] Coating treatment Root length (cm) Bud length (cm) Germination rate (%) No. 4 pollen polysaccharide seed coating agent (saline-alkali) 5.86b 4.58b 63.00b Seed coating agent No. 4 (without pollen polysaccharide and saline-alkali) 4.33c 3.56c 32.00c No coating (salt and alkali) 4.78c 3.89bc 51.00bc No coating (no salt or alkali) 11.4a 10.3a 95.00a

[0114] It can be seen from Table 11 that adding pollen polysaccharides to the seed coating agent can effectively alleviate the inhibitory effect of saline-alkali stress on wheat germination and growth, increase the germination rate, and promote wheat growth.

[0115] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A growth-promoting and stress-resistant seed coating agent containing pollen polysaccharide biostimulant, characterized in that: The following components are included by weight: Pollen polysaccharide extract 30-50%, film former 0.5-4%, antifreeze 3-5%, plant growth regulator 0.05-0.2%, fungicide 1-8%, insecticide 2-6%, colorant 0.01-0.2%, trace elements 1.1-2.4%, and the balance is water.

2. The seed coating agent according to claim 1, characterized in that The film-forming agent is selected from any one or more of sodium alginate, chitosan and sodium carboxymethyl cellulose.

3. The seed coating agent according to claim 1, characterized in that The antifreeze agent is selected from one or more of ethylene glycol, propylene glycol and urea.

4. The seed coating agent according to claim 1, characterized in that The plant growth regulator is selected from one or more of gibberellins and triacontanol.

5. The seed coating agent according to claim 1, characterized in that The bactericide is selected from any one or more of carbendazim, difenoconazole and meconazole.

6. The seed coating agent according to claim 1, characterized in that The insecticide is selected from any one or more of imidacloprid, acetamiprid and thiamethoxam.

7. The seed coating agent according to claim 1, characterized in that The colorant is selected from one or more of dye GRB and alkaline rose essence.

8. The seed coating agent according to claim 1, characterized in that The trace elements are selected from one or more of boron fertilizer, manganese fertilizer and zinc fertilizer; Furthermore, the boron fertilizer is selected from one or more of boric acid, sodium tetraborate, and borax, the manganese fertilizer is selected from one or more of manganese sulfate, manganese chloride, and EDTA-Mn, and the zinc fertilizer is selected from one or more of zinc sulfate, zinc chloride, and EDTA-Zn.

9. The method for preparing the seed coating agent according to any one of claims 1 to 8, characterized in that: The following steps are involved: 1) Weigh a certain amount of water in advance, add the pollen polysaccharide extract, stir evenly to make a solution; 2) Raising the temperature to 70-80° C., weighing a film-forming agent according to the mass percentage of the above formula, adding it to the above solution, stirring while adding until the film-forming agent is completely dissolved, to obtain a composite film-forming agent solution; 3) Weigh antifreeze agent, plant growth regulator, fungicide, insecticide, colorant and trace elements according to the mass percentage of the above formula, add them into the above composite film-forming agent solution, mix well, and obtain the target seed coating agent.

10. The preparation method according to claim 9, characterized in that: The dosage ratio of the seed coating agent to seeds is 100ml / 100kg to 400ml / 100kg.

11. The preparation method according to claim 9, characterized in that: The dosage ratio of the seed coating agent to seeds is 200-350 ml / 100 kg.

12. The seed coating agent according to any one of claims 1 to 8, characterized in that: The seed coating agent has the application of improving the growth-promoting and stress-resistance performance of plants under adverse environments, wherein the adverse environments include: one or more of saline-alkali, drought, and low temperature.

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